LOC100176229 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100176229
Entrez Gene ID	100176229
Full Name	acylamino-acid-releasing enzyme-like
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002121135.4	XP_002121171.1	acylamino-acid-releasing enzyme-like
XM_026837989.1	XP_026693790.1	acylamino-acid-releasing enzyme-like

Pathways from BioSystems
Gene Ontology

The following LOC100176229 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100176229 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi152274	XM_026837989.1 Latest version!	Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCi09674	XM_002121135.4 Latest version!	Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100176229 ( XM_026837989.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026693790.1 Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.

Price & Availability↑

CloneID	OCi152274
Clone ID Related Accession (Same CDS sequence)	XM_026837989.1
Accession Version	XM_026837989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	acylamino-acid-releasing enzyme-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004190367.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGTCACATG ACATCGTTGA CGGGAAAATT CAAAGCACAT CGTCGGTTCT TGTTGATGAG 
GTTAAAAACG ACAACTTTAG TTTCAATGGG ATGTTTATGG AGTCCATACC ATCTGACTGT 
TGGGGTAAAA ATGAATTAAT ATTCACAACA AATCATCGGA GTAATATTTC TATGTTTGCA 
TTCAATGTAA ACAATGGTGA TGTTCGTTGC TTAGCAACCG ACGGTGCATG GCGCGTCCAC 
AAAGTAGTTG ATGACATCAT AATAGCATCA TGCTCAACGC CTAACACCCC ATCTAGTGTG 
AAAGTTGGAT TGTTGTCCGA TGAAATTAAA TGGACGGACA TTGAAACCTC AACTATCATG 
AAGGATATTT CATGGGAGGT CATCCAACAC ACACCTAATC ATAAGAATAA TGATTTCCCC 
GGGTTAACTT TTGAATCCAT TCTCCTCAAA CCCACCCACA CCCCAATAAA AGGGTTGGTG 
GTTAACCCAC ATGGGGGTCC ACATGGTTGT TACCCAACAT CCTTTGATTT ACAATCGGCT 
GCACTTTGTA AGCTTGGCTT CGCTGTTTCA AGAATCAATT ACCGCGGCTC CACCGGATTC 
GGACAAAACT CGATATTTTC GCTTCCTGGA AATATTTCGA AGCAAGATGT TTTCGATGTC 
CAGCAGGTGG CAGAGTTTGT GTCGGAACGC TTAAATACAG GAGTGCGGTT TATTACGGGG 
GGGTCACATG GTGGGTTTCT TACCCTTCAA CTCATTGGAC AATTTCCTGA TTATTATTCT 
GCGGCTGCTG CTCGTAACCC GGTCACAAAC ATTGCTTCCA TAGTTGGCGT GTCCGATATT 
AGAGATTGGG CTTTTTGTGT TGCTGGGTGT AAGTTTACTT ACGATGCTTG CGTAACATCA 
GATATGTACA ATACAATGTT GACGGTATCG CCCATCATGC ATGTTGATGA TGTAAAAACC 
CCAGTGATGA TCATGTTAGG TGAAGCTGAC TTAAGAGTCC CAATCTCTCA ATCTCATGAA 
TACGCTCGCA TGTTGCGTGC CAGAGGTAAA ACTGTTCGCT TGTTTCAATA TAAAGACAAC 
AATCACCCGA TATCTAATGT GAAAGATGAA GCTGACTGTT TTGTGAACAT TCTCTCATGG 
TTCAACAATT ATTGTAAAAG TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026693790.1
CDS	1..1167
Translation	MSHDIVDGKIQSTSSVLVDEVKNDNFSFNGMFMESIPSDCWGKNELIFTTNHRSNISMFAFNVNNGDVRCLATDGAWRVHKVVDDIIIASCSTPNTPSSVKVGLLSDEIKWTDIETSTIMKDISWEVIQHTPNHKNNDFPGLTFESILLKPTHTPIKGLVVNPHGGPHGCYPTSFDLQSAALCKLGFAVSRINYRGSTGFGQNSIFSLPGNISKQDVFDVQQVAEFVSERLNTGVRFITGGSHGGFLTLQLIGQFPDYYSAAAARNPVTNIASIVGVSDIRDWAFCVAGCKFTYDACVTSDMYNTMLTVSPIMHVDDVKTPVMIMLGEADLRVPISQSHEYARMLRARGKTVRLFQYKDNNHPISNVKDEADCFVNILSWFNNYCKSE

Target ORF information:

RefSeq Version	XM_026837989.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026837989.1

ORF Insert Sequence:

ATGTCACATG ACATCGTTGA CGGGAAAATT CAAAGCACAT CGTCGGTTCT TGTTGATGAG 
GTTAAAAACG ACAACTTTAG TTTCAATGGG ATGTTTATGG AGTCCATACC ATCTGACTGT 
TGGGGTAAAA ATGAATTAAT ATTCACAACA AATCATCGGA GTAATATTTC TATGTTTGCA 
TTCAATGTAA ACAATGGTGA TGTTCGTTGC TTAGCAACCG ACGGTGCATG GCGCGTCCAC 
AAAGTAGTTG ATGACATCAT AATAGCATCA TGCTCAACGC CTAACACCCC ATCTAGTGTG 
AAAGTTGGAT TGTTGTCCGA TGAAATTAAA TGGACGGACA TTGAAACCTC AACTATCATG 
AAGGATATTT CATGGGAGGT CATCCAACAC ACACCTAATC ATAAGAATAA TGATTTCCCC 
GGGTTAACTT TTGAATCCAT TCTCCTCAAA CCCACCCACA CCCCAATAAA AGGGTTGGTG 
GTTAACCCAC ATGGGGGTCC ACATGGTTGT TACCCAACAT CCTTTGATTT ACAATCGGCT 
GCACTTTGTA AGCTTGGCTT CGCTGTTTCA AGAATCAATT ACCGCGGCTC CACCGGATTC 
GGACAAAACT CGATATTTTC GCTTCCTGGA AATATTTCGA AGCAAGATGT TTTCGATGTC 
CAGCAGGTGG CAGAGTTTGT GTCGGAACGC TTAAATACAG GAGTGCGGTT TATTACGGGG 
GGGTCACATG GTGGGTTTCT TACCCTTCAA CTCATTGGAC AATTTCCTGA TTATTATTCT 
GCGGCTGCTG CTCGTAACCC GGTCACAAAC ATTGCTTCCA TAGTTGGCGT GTCCGATATT 
AGAGATTGGG CTTTTTGTGT TGCTGGGTGT AAGTTTACTT ACGATGCTTG CGTAACATCA 
GATATGTACA ATACAATGTT GACGGTATCG CCCATCATGC ATGTTGATGA TGTAAAAACC 
CCAGTGATGA TCATGTTAGG TGAAGCTGAC TTAAGAGTCC CAATCTCTCA ATCTCATGAA 
TACGCTCGCA TGTTGCGTGC CAGAGGTAAA ACTGTTCGCT TGTTTCAATA TAAAGACAAC 
AATCACCCGA TATCTAATGT GAAAGATGAA GCTGACTGTT TTGTGAACAT TCTCTCATGG 
TTCAACAATT ATTGTAAAAG TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100176229 ( XM_002121135.4 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002121171.1 Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.

Price & Availability↑

CloneID	OCi09674
Clone ID Related Accession (Same CDS sequence)	XM_002121135.4
Accession Version	XM_002121135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	acylamino-acid-releasing enzyme-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004190367.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_002121135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTA TGGAAGACAA ATGTGTTTCT GTTTACCGCG AATTAAGTTT ATTACCGTCA 
GTACATTCGG TTGAAGTTGT TAAAAACAAT GGTGCGTTTC ATATTGATTG TGTTTGGGAG 
CATAACGATT TGGAAAGATT CGCTAAAATA AAATATCGGA AAAGTGGTTT ATTTAAAAAA 
TCGGGTGCTG GGTTCGAGGT GTATATGAGT CCTCTGTGTT CTGAGGTGAT AGGTGAACAA 
CTAATCTCAT TGTCTAAATG TGGTTTAAAA CGGGCGGTGC TACGTGAAGC CACGGTGGAT 
GGAAAAAGTC GGCAATATCT AGAAATCTGG GAAAATTCAT TAAAAGTTGC CACTGTTGAT 
TTTGTAGCAG AAAATAAACA TGGATTAGTT TATTCGAACG CGGTGTTTGG GTGTTTGGAA 
TGGTCACGTG ATGGTAGCAA AGTTCTTTAT ATTGCTGAGG AAAAAGTTAA TAAGTCTTGT 
TCCTATTTTA AAAAGGCTGC AAAAGATGAG ACGCCAGGTC TTGATGGTTT GCATTGTGAA 
GATTGGGGCG AGCAGCTGGT TGGGTGTTGT AAGACGTCAT TATATATCTA TGATGTGAAA 
AGATGCTGTA TATTATCTCT ATGTGATTTG TTTCCCCAAA ATATATCAAT CGGGAAAGGC 
CTTTGGAGCG ATGATGGTAA AAACATTATT GTCCTTGGAT GGAATGGATC GCCATGGAAG 
ATTGGTTTGA TTTACTGCAA GAACCGACCA AGTGCGTTGT ATCAACTTAA TCTTGACAAT 
AAAGAAGTTG TTCAAGTAAC CGACGGCGAC TACTGTGTGT ATTCGCCCAT CGTTAACCCG 
GCCGGGACAA AGTTACTTTA TTTACAAAGT CAACCATTTG GACCTCACCG ACAATGTGGG 
AAGTTAATGT CACATGACAT CGTTGACGGG AAAATTCAAA GCACATCGTC GGTTCTTGTT 
GATGAGGTTA AAAACGACAA CTTTAGTTTC AATGGGATGT TTATGGAGTC CATACCATCT 
GACTGTTGGG GTAAAAATGA ATTAATATTC ACAACAAATC ATCGGAGTAA TATTTCTATG 
TTTGCATTCA ATGTAAACAA TGGTGATGTT CGTTGCTTAG CAACCGACGG TGCATGGCGC 
GTCCACAAAG TAGTTGATGA CATCATAATA GCATCATGCT CAACGCCTAA CACCCCATCT 
AGTGTGAAAG TTGGATTGTT GTCCGATGAA ATTAAATGGA CGGACATTGA AACCTCAACT 
ATCATGAAGG ATATTTCATG GGAGGTCATC CAACACACAC CTAATCATAA GAATAATGAT 
TTCCCCGGGT TAACTTTTGA ATCCATTCTC CTCAAACCCA CCCACACCCC AATAAAAGGG 
TTGGTGGTTA ACCCACATGG GGGTCCACAT GGTTGTTACC CAACATCCTT TGATTTACAA 
TCGGCTGCAC TTTGTAAGCT TGGCTTCGCT GTTTCAAGAA TCAATTACCG CGGCTCCACC 
GGATTCGGAC AAAACTCGAT ATTTTCGCTT CCTGGAAATA TTTCGAAGCA AGATGTTTTC 
GATGTCCAGC AGGTGGCAGA GTTTGTGTCG GAACGCTTAA ATACAGGAGT GCGGTTTATT 
ACGGGGGGGT CACATGGTGG GTTTCTTACC CTTCAACTCA TTGGACAATT TCCTGATTAT 
TATTCTGCGG CTGCTGCTCG TAACCCGGTC ACAAACATTG CTTCCATAGT TGGCGTGTCC 
GATATTAGAG ATTGGGCTTT TTGTGTTGCT GGGTGTAAGT TTACTTACGA TGCTTGCGTA 
ACATCAGATA TGTACAATAC AATGTTGACG GTATCGCCCA TCATGCATGT TGATGATGTA 
AAAACCCCAG TGATGATCAT GTTAGGTGAA GCTGACTTAA GAGTCCCAAT CTCTCAATCT 
CATGAATACG CTCGCATGTT GCGTGCCAGA GGTAAAACTG TTCGCTTGTT TCAATATAAA 
GACAACAATC ACCCGATATC TAATGTGAAA GATGAAGCTG ACTGTTTTGT GAACATTCTC 
TCATGGTTCA ACAATTATTG TAAAAGTGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002121171.1
CDS	1065..3137
Translation	MATMEDKCVSVYRELSLLPSVHSVEVVKNNGAFHIDCVWEHNDLERFAKIKYRKSGLFKKSGAGFEVYMSPLCSEVIGEQLISLSKCGLKRAVLREATVDGKSRQYLEIWENSLKVATVDFVAENKHGLVYSNAVFGCLEWSRDGSKVLYIAEEKVNKSCSYFKKAAKDETPGLDGLHCEDWGEQLVGCCKTSLYIYDVKRCCILSLCDLFPQNISIGKGLWSDDGKNIIVLGWNGSPWKIGLIYCKNRPSALYQLNLDNKEVVQVTDGDYCVYSPIVNPAGTKLLYLQSQPFGPHRQCGKLMSHDIVDGKIQSTSSVLVDEVKNDNFSFNGMFMESIPSDCWGKNELIFTTNHRSNISMFAFNVNNGDVRCLATDGAWRVHKVVDDIIIASCSTPNTPSSVKVGLLSDEIKWTDIETSTIMKDISWEVIQHTPNHKNNDFPGLTFESILLKPTHTPIKGLVVNPHGGPHGCYPTSFDLQSAALCKLGFAVSRINYRGSTGFGQNSIFSLPGNISKQDVFDVQQVAEFVSERLNTGVRFITGGSHGGFLTLQLIGQFPDYYSAAAARNPVTNIASIVGVSDIRDWAFCVAGCKFTYDACVTSDMYNTMLTVSPIMHVDDVKTPVMIMLGEADLRVPISQSHEYARMLRARGKTVRLFQYKDNNHPISNVKDEADCFVNILSWFNNYCKSE

Target ORF information:

RefSeq Version	XM_002121135.4
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis acylamino-acid-releasing enzyme-like (LOC100176229), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002121135.4

ORF Insert Sequence:

ATGGCAACTA TGGAAGACAA ATGTGTTTCT GTTTACCGCG AATTAAGTTT ATTACCGTCA 
GTACATTCGG TTGAAGTTGT TAAAAACAAT GGTGCGTTTC ATATTGATTG TGTTTGGGAG 
CATAACGATT TGGAAAGATT CGCTAAAATA AAATATCGGA AAAGTGGTTT ATTTAAAAAA 
TCGGGTGCTG GGTTCGAGGT GTATATGAGT CCTCTGTGTT CTGAGGTGAT AGGTGAACAA 
CTAATCTCAT TGTCTAAATG TGGTTTAAAA CGGGCGGTGC TACGTGAAGC CACGGTGGAT 
GGAAAAAGTC GGCAATATCT AGAAATCTGG GAAAATTCAT TAAAAGTTGC CACTGTTGAT 
TTTGTAGCAG AAAATAAACA TGGATTAGTT TATTCGAACG CGGTGTTTGG GTGTTTGGAA 
TGGTCACGTG ATGGTAGCAA AGTTCTTTAT ATTGCTGAGG AAAAAGTTAA TAAGTCTTGT 
TCCTATTTTA AAAAGGCTGC AAAAGATGAG ACGCCAGGTC TTGATGGTTT GCATTGTGAA 
GATTGGGGCG AGCAGCTGGT TGGGTGTTGT AAGACGTCAT TATATATCTA TGATGTGAAA 
AGATGCTGTA TATTATCTCT ATGTGATTTG TTTCCCCAAA ATATATCAAT CGGGAAAGGC 
CTTTGGAGCG ATGATGGTAA AAACATTATT GTCCTTGGAT GGAATGGATC GCCATGGAAG 
ATTGGTTTGA TTTACTGCAA GAACCGACCA AGTGCGTTGT ATCAACTTAA TCTTGACAAT 
AAAGAAGTTG TTCAAGTAAC CGACGGCGAC TACTGTGTGT ATTCGCCCAT CGTTAACCCG 
GCCGGGACAA AGTTACTTTA TTTACAAAGT CAACCATTTG GACCTCACCG ACAATGTGGG 
AAGTTAATGT CACATGACAT CGTTGACGGG AAAATTCAAA GCACATCGTC GGTTCTTGTT 
GATGAGGTTA AAAACGACAA CTTTAGTTTC AATGGGATGT TTATGGAGTC CATACCATCT 
GACTGTTGGG GTAAAAATGA ATTAATATTC ACAACAAATC ATCGGAGTAA TATTTCTATG 
TTTGCATTCA ATGTAAACAA TGGTGATGTT CGTTGCTTAG CAACCGACGG TGCATGGCGC 
GTCCACAAAG TAGTTGATGA CATCATAATA GCATCATGCT CAACGCCTAA CACCCCATCT 
AGTGTGAAAG TTGGATTGTT GTCCGATGAA ATTAAATGGA CGGACATTGA AACCTCAACT 
ATCATGAAGG ATATTTCATG GGAGGTCATC CAACACACAC CTAATCATAA GAATAATGAT 
TTCCCCGGGT TAACTTTTGA ATCCATTCTC CTCAAACCCA CCCACACCCC AATAAAAGGG 
TTGGTGGTTA ACCCACATGG GGGTCCACAT GGTTGTTACC CAACATCCTT TGATTTACAA 
TCGGCTGCAC TTTGTAAGCT TGGCTTCGCT GTTTCAAGAA TCAATTACCG CGGCTCCACC 
GGATTCGGAC AAAACTCGAT ATTTTCGCTT CCTGGAAATA TTTCGAAGCA AGATGTTTTC 
GATGTCCAGC AGGTGGCAGA GTTTGTGTCG GAACGCTTAA ATACAGGAGT GCGGTTTATT 
ACGGGGGGGT CACATGGTGG GTTTCTTACC CTTCAACTCA TTGGACAATT TCCTGATTAT 
TATTCTGCGG CTGCTGCTCG TAACCCGGTC ACAAACATTG CTTCCATAGT TGGCGTGTCC 
GATATTAGAG ATTGGGCTTT TTGTGTTGCT GGGTGTAAGT TTACTTACGA TGCTTGCGTA 
ACATCAGATA TGTACAATAC AATGTTGACG GTATCGCCCA TCATGCATGT TGATGATGTA 
AAAACCCCAG TGATGATCAT GTTAGGTGAA GCTGACTTAA GAGTCCCAAT CTCTCAATCT 
CATGAATACG CTCGCATGTT GCGTGCCAGA GGTAAAACTG TTCGCTTGTT TCAATATAAA 
GACAACAATC ACCCGATATC TAATGTGAAA GATGAAGCTG ACTGTTTTGT GAACATTCTC 
TCATGGTTCA ACAATTATTG TAAAAGTGAA TAA