LOC101463274 cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101463274
Entrez Gene ID	101463274
Full Name	E3 ubiquitin ligase Rnf157
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012305000.2	XP_012160390.1	E3 ubiquitin ligase Rnf157

Pathways from BioSystems
Gene Ontology

The following LOC101463274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101463274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe175223	XM_012305000.2 Latest version!	Ceratitis capitata E3 ubiquitin ligase Rnf157 (LOC101463274), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101463274 ( XM_012305000.2 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_012160390.1 Ceratitis capitata E3 ubiquitin ligase Rnf157 (LOC101463274), mRNA.

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CloneID	OCe175223
Clone ID Related Accession (Same CDS sequence)	XM_012305000.2
Accession Version	XM_012305000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	E3 ubiquitin ligase Rnf157
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376232.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 21, 2017 this sequence version replaced XM_012305000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAT TTACCAGTCG TCAGAATGGC GCAGTTGAGG AGGCAGATCA GCGTAATTCA 
ACTCATGCCT ACAAATATCC ACCGCGTTCA GGTAACTTCT TCGGCACCCA CTTTATTATG 
GGCGGAGAGC GCTTCGATAC ACCCCAGCCC GAGTCCTATT TGTTTGGTGA AAATGCTGAC 
CTGAATTTTC TTGGTAGTCG GCCTACAGCA TTTCCTTACC CTCCCCCACA AGCAAGTGAG 
CCAACAAAAA CATTGAAAAG TCTGGTAAAC ATACGAAAAG AATCTGTGCG TTTTCTACGA 
GTTTCGTCTG AAAAGCAAGA AATAGTGACT GCTAATACTA CAAATCTTGA TGGCATCGAT 
GGTAATGTAC TTACCAGTGA AATAGGTGGT AGCGGAGATG GTGCTTTGCA ATATGGTTCA 
TTTTCATATA ATATCGAATT CACATTCGAC TCCGATGCAA AGTGCGCTAT CACTATATAT 
TATTTCTGCA CTGAGGAAGT TAGTCCAAGT GGAGTAACAC TTGTACCACG AGATGGGTTA 
ACTTCCGAAA CTTATCACTA TGGCAAAGGC ATAAATCAAT TTTTCTCGCA GTCAAGCCAT 
GTGTTTAATC CGCAATTAAT TCCTGAAGAT GATTTGGTCT ACAACCCGAC AAAAGAACAG 
TATCCGGTCG CTATACATTG TGTAGTCGAA GAAGGCAACG AAGATTGTCG ACAATCGCAT 
ACAACTATTT GTGTTATTGA TCATCATCCA GAAACCAATA GCTACGTTTT GCGGGCCCTG 
AAACAGAAAA TATTTGTTGA TGGTCTGTGT TATCTGTTGC AAGAAATATA TGGCATCGAA 
AATAAAGCTG TGAACAAGAG CTCCATCGAT GAGGATATTG ATGATCACGG CAGCGAATGT 
GTTATTTGTA TGAGTGAAAC TAGAGACACT CTTATATTAC CTTGTCGACA TCTGTGCCTA 
TGCAACTCAT GTGCAGATTC TCTGCGTTAT CAGGCAAATA ATTGTCCCAT TTGCCGAGCA 
CCTTTCCGTG CACTTCTCCA AATACGCGCT GTCCAAAAAG GAGTCAGTTC TCATGCTTTA 
CTCCAAAACA CGCCTACGTC GGCTCCAGGA GAAACAGCAC CTTGTGATCA ACATATACCA 
CCAGGTTATA TACCAGTATC ACTCATAGAA GCACTCAATG GACCGCCACA ATTTGTTTGT 
AGACCTCGAA CGAATGATGG TGACATGATT GACGGTGTAC CTCATACCGC TGTATCCGTC 
GCAGATAATC ATAAAGCAAC ATCTCCATTA AAACCAAACC AACGTCGAAC TAAGATAAAA 
AAAAATGCCT CGACTTCAAC AAATACCAGT GAAATAGGAA CAATCACAGC GACTAGCAAT 
GCTAATAATC AAGCGCTCTC TGTAGTGGAA ATAACAAACG AAATTAAGGT AAACACTTCA 
GCTGGCGAAG GATTAGGTTC TACAGCGACA CAAAAGAAAT CTCTTGCTAC GGAGAAAACA 
GAAACACCCC ATAATAATGC CAACCATACG CAAGATAAAT TGCCAATTGA AAGCGAACGT 
TCTAGTTTAA AGATGCCAAA AATCAAACGC ACTTCCAATA CAAATAGTGC AAACTCATCA 
TCATCACAAA ATGATCTGGA CGACGACGAT AGTGAAAATG AAAAATTATC ACCCCTTCTG 
TCCCCTGGTA CTAAGCCGAT AAAAGCAACA AATGCTGCGC GGAGTAAAGA AGCAGTTGTT 
GTTACCAATG ACAGTGATAA CGATGTAGTT GATAGCTTAG AGATGAAAGC TGTAGTTTCA 
ATGAAAAAGA GCACATCCGG CAGTAATGCC GAGTCTGTAG CTGGTGGCAA TGCAGACGAC 
TCAGATTATT ACACACCAGA AGACACGCAG AACTCGATAT TGAGTCCATT ATGTCCCGAT 
CGAGAAAGAG AACGCTCAAA GAGCGGCGAC AAGTTAGGTA GTTTGAAAAG TAACGTTGGC 
AGTTTTAAAG GCGCCAATAA CTTAGCTAAA AAGAATCTTC ATAAGAAATC TACGTCGGTG 
GGAAATATAA TTGGGCCGGG AAGTAGTGCA TCAGTAAACT GTGGAATAGC TGGCAACATA 
GTTGGTGATA TTAAAACGAA CGTGAGCTCT CTGCCAGACA TGTTAGATAA CCCAATAAAC 
GGCAATACGG CATCTACCCG CAGTTCTAGT GACAGCTATT CATCTAGTTC GTCCACCAAA 
CAGCTACTAA GTTCCAATCC GGCTAATGTG ACAGCTAATA TCATAGTTGA TGACAAGGTT 
GCTCTTCAAA ATGCAGTAAA TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012160390.1
CDS	232..2538
Translation	MGAFTSRQNGAVEEADQRNSTHAYKYPPRSGNFFGTHFIMGGERFDTPQPESYLFGENADLNFLGSRPTAFPYPPPQASEPTKTLKSLVNIRKESVRFLRVSSEKQEIVTANTTNLDGIDGNVLTSEIGGSGDGALQYGSFSYNIEFTFDSDAKCAITIYYFCTEEVSPSGVTLVPRDGLTSETYHYGKGINQFFSQSSHVFNPQLIPEDDLVYNPTKEQYPVAIHCVVEEGNEDCRQSHTTICVIDHHPETNSYVLRALKQKIFVDGLCYLLQEIYGIENKAVNKSSIDEDIDDHGSECVICMSETRDTLILPCRHLCLCNSCADSLRYQANNCPICRAPFRALLQIRAVQKGVSSHALLQNTPTSAPGETAPCDQHIPPGYIPVSLIEALNGPPQFVCRPRTNDGDMIDGVPHTAVSVADNHKATSPLKPNQRRTKIKKNASTSTNTSEIGTITATSNANNQALSVVEITNEIKVNTSAGEGLGSTATQKKSLATEKTETPHNNANHTQDKLPIESERSSLKMPKIKRTSNTNSANSSSSQNDLDDDDSENEKLSPLLSPGTKPIKATNAARSKEAVVVTNDSDNDVVDSLEMKAVVSMKKSTSGSNAESVAGGNADDSDYYTPEDTQNSILSPLCPDRERERSKSGDKLGSLKSNVGSFKGANNLAKKNLHKKSTSVGNIIGPGSSASVNCGIAGNIVGDIKTNVSSLPDMLDNPINGNTASTRSSSDSYSSSSSTKQLLSSNPANVTANIIVDDKVALQNAVNV

Target ORF information:

RefSeq Version	XM_012305000.2
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata E3 ubiquitin ligase Rnf157 (LOC101463274), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012305000.2

ORF Insert Sequence:

ATGGGTGCAT TTACCAGTCG TCAGAATGGC GCAGTTGAGG AGGCAGATCA GCGTAATTCA 
ACTCATGCCT ACAAATATCC ACCGCGTTCA GGTAACTTCT TCGGCACCCA CTTTATTATG 
GGCGGAGAGC GCTTCGATAC ACCCCAGCCC GAGTCCTATT TGTTTGGTGA AAATGCTGAC 
CTGAATTTTC TTGGTAGTCG GCCTACAGCA TTTCCTTACC CTCCCCCACA AGCAAGTGAG 
CCAACAAAAA CATTGAAAAG TCTGGTAAAC ATACGAAAAG AATCTGTGCG TTTTCTACGA 
GTTTCGTCTG AAAAGCAAGA AATAGTGACT GCTAATACTA CAAATCTTGA TGGCATCGAT 
GGTAATGTAC TTACCAGTGA AATAGGTGGT AGCGGAGATG GTGCTTTGCA ATATGGTTCA 
TTTTCATATA ATATCGAATT CACATTCGAC TCCGATGCAA AGTGCGCTAT CACTATATAT 
TATTTCTGCA CTGAGGAAGT TAGTCCAAGT GGAGTAACAC TTGTACCACG AGATGGGTTA 
ACTTCCGAAA CTTATCACTA TGGCAAAGGC ATAAATCAAT TTTTCTCGCA GTCAAGCCAT 
GTGTTTAATC CGCAATTAAT TCCTGAAGAT GATTTGGTCT ACAACCCGAC AAAAGAACAG 
TATCCGGTCG CTATACATTG TGTAGTCGAA GAAGGCAACG AAGATTGTCG ACAATCGCAT 
ACAACTATTT GTGTTATTGA TCATCATCCA GAAACCAATA GCTACGTTTT GCGGGCCCTG 
AAACAGAAAA TATTTGTTGA TGGTCTGTGT TATCTGTTGC AAGAAATATA TGGCATCGAA 
AATAAAGCTG TGAACAAGAG CTCCATCGAT GAGGATATTG ATGATCACGG CAGCGAATGT 
GTTATTTGTA TGAGTGAAAC TAGAGACACT CTTATATTAC CTTGTCGACA TCTGTGCCTA 
TGCAACTCAT GTGCAGATTC TCTGCGTTAT CAGGCAAATA ATTGTCCCAT TTGCCGAGCA 
CCTTTCCGTG CACTTCTCCA AATACGCGCT GTCCAAAAAG GAGTCAGTTC TCATGCTTTA 
CTCCAAAACA CGCCTACGTC GGCTCCAGGA GAAACAGCAC CTTGTGATCA ACATATACCA 
CCAGGTTATA TACCAGTATC ACTCATAGAA GCACTCAATG GACCGCCACA ATTTGTTTGT 
AGACCTCGAA CGAATGATGG TGACATGATT GACGGTGTAC CTCATACCGC TGTATCCGTC 
GCAGATAATC ATAAAGCAAC ATCTCCATTA AAACCAAACC AACGTCGAAC TAAGATAAAA 
AAAAATGCCT CGACTTCAAC AAATACCAGT GAAATAGGAA CAATCACAGC GACTAGCAAT 
GCTAATAATC AAGCGCTCTC TGTAGTGGAA ATAACAAACG AAATTAAGGT AAACACTTCA 
GCTGGCGAAG GATTAGGTTC TACAGCGACA CAAAAGAAAT CTCTTGCTAC GGAGAAAACA 
GAAACACCCC ATAATAATGC CAACCATACG CAAGATAAAT TGCCAATTGA AAGCGAACGT 
TCTAGTTTAA AGATGCCAAA AATCAAACGC ACTTCCAATA CAAATAGTGC AAACTCATCA 
TCATCACAAA ATGATCTGGA CGACGACGAT AGTGAAAATG AAAAATTATC ACCCCTTCTG 
TCCCCTGGTA CTAAGCCGAT AAAAGCAACA AATGCTGCGC GGAGTAAAGA AGCAGTTGTT 
GTTACCAATG ACAGTGATAA CGATGTAGTT GATAGCTTAG AGATGAAAGC TGTAGTTTCA 
ATGAAAAAGA GCACATCCGG CAGTAATGCC GAGTCTGTAG CTGGTGGCAA TGCAGACGAC 
TCAGATTATT ACACACCAGA AGACACGCAG AACTCGATAT TGAGTCCATT ATGTCCCGAT 
CGAGAAAGAG AACGCTCAAA GAGCGGCGAC AAGTTAGGTA GTTTGAAAAG TAACGTTGGC 
AGTTTTAAAG GCGCCAATAA CTTAGCTAAA AAGAATCTTC ATAAGAAATC TACGTCGGTG 
GGAAATATAA TTGGGCCGGG AAGTAGTGCA TCAGTAAACT GTGGAATAGC TGGCAACATA 
GTTGGTGATA TTAAAACGAA CGTGAGCTCT CTGCCAGACA TGTTAGATAA CCCAATAAAC 
GGCAATACGG CATCTACCCG CAGTTCTAGT GACAGCTATT CATCTAGTTC GTCCACCAAA 
CAGCTACTAA GTTCCAATCC GGCTAATGTG ACAGCTAATA TCATAGTTGA TGACAAGGTT 
GCTCTTCAAA ATGCAGTAAA TGTTTAA