LOC101462148 cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101462148
Entrez Gene ID	101462148
Full Name	cysteine protease ATG4B
Synonyms	Atg4b
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004521851.3	XP_004521908.1	cysteine protease ATG4B isoform X2
XM_004521850.3	XP_004521907.1	cysteine protease ATG4B isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101462148 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101462148 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe178216	XM_004521850.3 Latest version!	Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe178217	XM_004521851.3 Latest version!	Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101462148 ( XM_004521850.3 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_004521907.1 Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe178216
Clone ID Related Accession (Same CDS sequence)	XM_004521850.3
Accession Version	XM_004521850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	cysteine protease ATG4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 21, 2017 this sequence version replaced XM_004521850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTG TATTCGAAGC ATACTTGGGC CCTGATGGAA TGCTCGCTGG AGCTGTGGCT 
GGTGCAGTTG GGGAACCAGA TGACATACCA AAAGTAAACA CCGATGTTTG GGTGTTGGGC 
AAACGTTATA ATGCCATACA AGAATTGGAA TTAATACGCC GCGACATACA ATCACGCCTC 
TGGTGCACTT ATCGTCGCGG TTTCGTGCCC TTGGGGGCAC CACAATTGAC TACCGACAAA 
GGTTGGGGCT GTATGCTGCG TTGTGGTCAA ATGGTCTTGG CACAAGCTCT GATAGATCTG 
CATTTGGGAC GTGAATGGTT TTGGACACCG GAGATACGTG ATGGGACATA TTTAAAAATA 
GTTAACCGTT TTGAGGATTC ACGCAAAAGT TACTTCTCAA TACACCAGAT CGCTTTGATG 
GGTGATTCGG AAGATAAAAA AGTAGGCCAA TGGTTCGGGC CAAACACTGT GGCGCAGGTG 
TTGAAGAAAC TTGTACGCTA CGACGACTGG TGCTCGTTAG TTATACATGT AGCCATGGAT 
AACACAATAG TGACTGATGA TATATATTCA CTATGCCTTG AAAACCCAAG TAATGATGAT 
ACTTGGAAAC CACTCCTACT CATAATTCCA CTGCGTTTAG GTCTCAGTGA TATAAATCCC 
ATTTATATAC CAGCACTGAA AATCTGTTTT GAGCTCGCTG GCAGTTGTGG CATGATTGGT 
GGTCGTCCAA ATCAAGCACT ATACTTTGTG GGTTATGTAG ATGATGAAGT TTTATATCTC 
GATCCACATA CCACGCAGCG TTCAGGTTCG GTGGGGCAAA AAACGTCACA GGATGAGGTG 
GATTTTGATG CAACGTTTCA TCAAAGGTAC GCAGGGCGCA TAGGTTTTCA ACAGATGGAT 
CCATCAATGG CGATGTGCTT CCTCTGCAAG TCACGCTTGA GTTTCGAAGT CTTACTTGAT 
AATCTGCGCA CACAAGTTTT GCCCGCCTGC AAACAACCAC TGTTCGAAAT TACAAAATCC 
CGCTCAGCGG AATGGGTTTC AACATCGTCA GGTATTGAGT CAAATTTTAC TACACTTAAC 
ACAGCGGGTT TGGTAAATTA TAATGCCGCA GCAACTACAA CGTCAACAAC AGCGTCGAAC 
GCCGCTTCCA GTGATGCCAC AAATAGTAAC ACGCCGCGTA CAACTATAGC AAAAACAATA 
GCTACGTCTG CACGTGCTAC TGGCGCAACA GCAGGAGCGG TGGCGGCCAT AGAAATTTAT 
AAGAATCGTT TTAATCGAAC TATAAGTTCT TGTGATTCTG AAGATTTTGA AAATGTTAAT 
CACTTACATA CAAGCAGCTT GACGCGCAAT GGTTGCGATG CTGATTCAGA GGCCGACTCA 
GATGATGATT TTGAAATAAT TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004521907.1
CDS	562..1968
Translation	MDSVFEAYLGPDGMLAGAVAGAVGEPDDIPKVNTDVWVLGKRYNAIQELELIRRDIQSRLWCTYRRGFVPLGAPQLTTDKGWGCMLRCGQMVLAQALIDLHLGREWFWTPEIRDGTYLKIVNRFEDSRKSYFSIHQIALMGDSEDKKVGQWFGPNTVAQVLKKLVRYDDWCSLVIHVAMDNTIVTDDIYSLCLENPSNDDTWKPLLLIIPLRLGLSDINPIYIPALKICFELAGSCGMIGGRPNQALYFVGYVDDEVLYLDPHTTQRSGSVGQKTSQDEVDFDATFHQRYAGRIGFQQMDPSMAMCFLCKSRLSFEVLLDNLRTQVLPACKQPLFEITKSRSAEWVSTSSGIESNFTTLNTAGLVNYNAAATTTSTTASNAASSDATNSNTPRTTIAKTIATSARATGATAGAVAAIEIYKNRFNRTISSCDSEDFENVNHLHTSSLTRNGCDADSEADSDDDFEIIS

Target ORF information:

RefSeq Version	XM_004521850.3
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004521850.3

ORF Insert Sequence:

ATGGATAGTG TATTCGAAGC ATACTTGGGC CCTGATGGAA TGCTCGCTGG AGCTGTGGCT 
GGTGCAGTTG GGGAACCAGA TGACATACCA AAAGTAAACA CCGATGTTTG GGTGTTGGGC 
AAACGTTATA ATGCCATACA AGAATTGGAA TTAATACGCC GCGACATACA ATCACGCCTC 
TGGTGCACTT ATCGTCGCGG TTTCGTGCCC TTGGGGGCAC CACAATTGAC TACCGACAAA 
GGTTGGGGCT GTATGCTGCG TTGTGGTCAA ATGGTCTTGG CACAAGCTCT GATAGATCTG 
CATTTGGGAC GTGAATGGTT TTGGACACCG GAGATACGTG ATGGGACATA TTTAAAAATA 
GTTAACCGTT TTGAGGATTC ACGCAAAAGT TACTTCTCAA TACACCAGAT CGCTTTGATG 
GGTGATTCGG AAGATAAAAA AGTAGGCCAA TGGTTCGGGC CAAACACTGT GGCGCAGGTG 
TTGAAGAAAC TTGTACGCTA CGACGACTGG TGCTCGTTAG TTATACATGT AGCCATGGAT 
AACACAATAG TGACTGATGA TATATATTCA CTATGCCTTG AAAACCCAAG TAATGATGAT 
ACTTGGAAAC CACTCCTACT CATAATTCCA CTGCGTTTAG GTCTCAGTGA TATAAATCCC 
ATTTATATAC CAGCACTGAA AATCTGTTTT GAGCTCGCTG GCAGTTGTGG CATGATTGGT 
GGTCGTCCAA ATCAAGCACT ATACTTTGTG GGTTATGTAG ATGATGAAGT TTTATATCTC 
GATCCACATA CCACGCAGCG TTCAGGTTCG GTGGGGCAAA AAACGTCACA GGATGAGGTG 
GATTTTGATG CAACGTTTCA TCAAAGGTAC GCAGGGCGCA TAGGTTTTCA ACAGATGGAT 
CCATCAATGG CGATGTGCTT CCTCTGCAAG TCACGCTTGA GTTTCGAAGT CTTACTTGAT 
AATCTGCGCA CACAAGTTTT GCCCGCCTGC AAACAACCAC TGTTCGAAAT TACAAAATCC 
CGCTCAGCGG AATGGGTTTC AACATCGTCA GGTATTGAGT CAAATTTTAC TACACTTAAC 
ACAGCGGGTT TGGTAAATTA TAATGCCGCA GCAACTACAA CGTCAACAAC AGCGTCGAAC 
GCCGCTTCCA GTGATGCCAC AAATAGTAAC ACGCCGCGTA CAACTATAGC AAAAACAATA 
GCTACGTCTG CACGTGCTAC TGGCGCAACA GCAGGAGCGG TGGCGGCCAT AGAAATTTAT 
AAGAATCGTT TTAATCGAAC TATAAGTTCT TGTGATTCTG AAGATTTTGA AAATGTTAAT 
CACTTACATA CAAGCAGCTT GACGCGCAAT GGTTGCGATG CTGATTCAGA GGCCGACTCA 
GATGATGATT TTGAAATAAT TTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101462148 ( XM_004521851.3 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_004521908.1 Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe178217
Clone ID Related Accession (Same CDS sequence)	XM_004521851.3
Accession Version	XM_004521851.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	cysteine protease ATG4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376448.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 21, 2017 this sequence version replaced XM_004521851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGTG TATTCGAAGC ATACTTGGGC CCTGATGGAA TGCTCGCTGG AGCTGTGGCT 
GGTGCAGTTG GGGAACCAGA TGACATACCA AAAGTAAACA CCGATGTTTG GGTGTTGGGC 
AAACGTTATA ATGCCATACA AGAATTGGAA TTAATACGCC GCGACATACA ATCACGCCTC 
TGGTGCACTT ATCGTCGCGG TTTCGTGCCC TTGGGGGCAC CACAATTGAC TACCGACAAA 
GGTTGGGGCT GTATGCTGCG TTGTGGTCAA ATGGTCTTGG CACAAGCTCT GATAGATCTG 
CATTTGGGAC GTGAATGGTT TTGGACACCG GAGATACGTG ATGGGACATA TTTAAAAATA 
GTTAACCGTT TTGAGGATTC ACGCAAAAGT TACTTCTCAA TACACCAGAT CGCTTTGATG 
GGTGATTCGG AAGATAAAAA AGTAGGCCAA TGGTTCGGGC CAAACACTGT GGCGCAGGTG 
TTGAAGAAAC TTGTACGCTA CGACGACTGG TGCTCGTTAG TTATACATGT AGCCATGGAT 
AACACAATAG TGACTGATGA TATATATTCA CTATGCCTTG AAAACCCAAG TAATGATGAT 
ACTTGGAAAC CACTCCTACT CATAATTCCA CTGCGTTTAG GTCTCAGTGA TATAAATCCC 
ATTTATATAC CAGCACTGAA AATCTGTTTT GAGCTCGCTG GCAGTTGTGG CATGATTGGT 
GGTCGTCCAA ATCAAGCACT ATACTTTGTG GGTTATGTAG ATGATGAAGT TTTATATCTC 
GATCCACATA CCACGCAGCG TTCAGGTTCG GTGGGGCAAA AAACGTCACA GGATGAGGTG 
GATTTTGATG CAACGTTTCA TCAAAGGTAC GCAGGGCGCA TAGGTTTTCA ACAGATGGAT 
CCATCAATGG CGATGTGCTT CCTCTGCAAG TCACGCTTGA GTTTCGAAGT CTTACTTGAT 
AATCTGCGCA CACAAGTTTT GCCCGCCTGC AAACAACCAC TGTTCGAAAT TACAAAATCC 
CGCTCAGCGG AATGGGTTTC AACATCGTCA GATTTTGAAA ATGTTAATCA CTTACATACA 
AGCAGCTTGA CGCGCAATGG TTGCGATGCT GATTCAGAGG CCGACTCAGA TGATGATTTT 
GAAATAATTT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004521908.1
CDS	561..1715
Translation	MDSVFEAYLGPDGMLAGAVAGAVGEPDDIPKVNTDVWVLGKRYNAIQELELIRRDIQSRLWCTYRRGFVPLGAPQLTTDKGWGCMLRCGQMVLAQALIDLHLGREWFWTPEIRDGTYLKIVNRFEDSRKSYFSIHQIALMGDSEDKKVGQWFGPNTVAQVLKKLVRYDDWCSLVIHVAMDNTIVTDDIYSLCLENPSNDDTWKPLLLIIPLRLGLSDINPIYIPALKICFELAGSCGMIGGRPNQALYFVGYVDDEVLYLDPHTTQRSGSVGQKTSQDEVDFDATFHQRYAGRIGFQQMDPSMAMCFLCKSRLSFEVLLDNLRTQVLPACKQPLFEITKSRSAEWVSTSSDFENVNHLHTSSLTRNGCDADSEADSDDDFEIIS

Target ORF information:

RefSeq Version	XM_004521851.3
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata cysteine protease ATG4B (LOC101462148), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004521851.3

ORF Insert Sequence:

ATGGATAGTG TATTCGAAGC ATACTTGGGC CCTGATGGAA TGCTCGCTGG AGCTGTGGCT 
GGTGCAGTTG GGGAACCAGA TGACATACCA AAAGTAAACA CCGATGTTTG GGTGTTGGGC 
AAACGTTATA ATGCCATACA AGAATTGGAA TTAATACGCC GCGACATACA ATCACGCCTC 
TGGTGCACTT ATCGTCGCGG TTTCGTGCCC TTGGGGGCAC CACAATTGAC TACCGACAAA 
GGTTGGGGCT GTATGCTGCG TTGTGGTCAA ATGGTCTTGG CACAAGCTCT GATAGATCTG 
CATTTGGGAC GTGAATGGTT TTGGACACCG GAGATACGTG ATGGGACATA TTTAAAAATA 
GTTAACCGTT TTGAGGATTC ACGCAAAAGT TACTTCTCAA TACACCAGAT CGCTTTGATG 
GGTGATTCGG AAGATAAAAA AGTAGGCCAA TGGTTCGGGC CAAACACTGT GGCGCAGGTG 
TTGAAGAAAC TTGTACGCTA CGACGACTGG TGCTCGTTAG TTATACATGT AGCCATGGAT 
AACACAATAG TGACTGATGA TATATATTCA CTATGCCTTG AAAACCCAAG TAATGATGAT 
ACTTGGAAAC CACTCCTACT CATAATTCCA CTGCGTTTAG GTCTCAGTGA TATAAATCCC 
ATTTATATAC CAGCACTGAA AATCTGTTTT GAGCTCGCTG GCAGTTGTGG CATGATTGGT 
GGTCGTCCAA ATCAAGCACT ATACTTTGTG GGTTATGTAG ATGATGAAGT TTTATATCTC 
GATCCACATA CCACGCAGCG TTCAGGTTCG GTGGGGCAAA AAACGTCACA GGATGAGGTG 
GATTTTGATG CAACGTTTCA TCAAAGGTAC GCAGGGCGCA TAGGTTTTCA ACAGATGGAT 
CCATCAATGG CGATGTGCTT CCTCTGCAAG TCACGCTTGA GTTTCGAAGT CTTACTTGAT 
AATCTGCGCA CACAAGTTTT GCCCGCCTGC AAACAACCAC TGTTCGAAAT TACAAAATCC 
CGCTCAGCGG AATGGGTTTC AACATCGTCA GATTTTGAAA ATGTTAATCA CTTACATACA 
AGCAGCTTGA CGCGCAATGG TTGCGATGCT GATTCAGAGG CCGACTCAGA TGATGATTTT 
GAAATAATTT CATAG