LOC101454443 cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101454443
Entrez Gene ID	101454443
Full Name	cysteine--tRNA ligase, mitochondrial
Synonyms	SYCM
Gene Type	protein-coding
Organism	Ceratitis capitata(Mediterranean fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004522886.3	XP_004522943.1	cysteine--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC101454443 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101454443 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe179228	XM_004522886.3 Latest version!	Ceratitis capitata cysteine--tRNA ligase, mitochondrial (LOC101454443), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101454443 ( XM_004522886.3 ) cDNA ORF clone, Ceratitis capitata(Mediterranean fruit fly) -> XP_004522943.1 Ceratitis capitata cysteine--tRNA ligase, mitochondrial (LOC101454443), mRNA.

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CloneID	OCe179228
Clone ID Related Accession (Same CDS sequence)	XM_004522886.3
Accession Version	XM_004522886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Ceratitis capitata(Mediterranean fruit fly)
Product	cysteine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 21, 2017 this sequence version replaced XM_004522886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratitis capitata Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCT CCAGTTACTT GAAGCGGTTC AGTTTCCTCT CACTACAAAG TAGATACCTT 
TGTAGTCAAC ACCAATGGAA ACAACCGAGC GAAGGGAGAG ATACAGGCAT ACGGATTTAT 
AATTGTGTTG CTCGAAAGCA AGTACCCCTA ATACTTTCCA ACGATGCATA TGCAACTTGG 
TATACATGTG GGCCCACTGT TTATGACTTC ACACATATTG GACACATCAG TTGCTATGTT 
AAATTAGACA TTATACAACG TATTTTGCGC CACCATTTCA ATGTGAATTT AGTAACGGCT 
ATGAATATAA CTGATATAGA TGATAAAATT ATACAGAAAA GTATAGAAAG TGGTAAACCG 
TGGCATGAGT TATCTCGTTT CTACGAAAAC GAATTCTGGT CGGTTCTTAA GCAACTAAAT 
GTGCAAGAGC CAAATATAAA AGTACGCGTC ACTGAGAATA TACCACGAAT TGTCGAATTT 
ATCACGCGCA TCATAGGAAA AGGTAGTGCT TACAAAGGTA ACGATGGCAG TGTATACTTT 
GCCGTCAATA CATGCAGAAA CTATGGTAAA CTGCAAAAGA CAAATATAGA TGAAGACCCG 
GAAAAGCCAA TACTTGGTGT AGAAGGCAAA ACGGCTCCCG CGGATTTTGC TCTATGGAAG 
GCACAAAAAT GTGAAAATGA ACCAGCTTGG CCATCACCTT GGGGCCCAGG ACGTCCAGGT 
TGGCATATTG AATGTAGCAC TTTGGCCAGC ATGATATTTG GACAACAACT GGACTTTCAT 
GCCGGAGGCT TAGACTTACG TTTTCCTCAT CACGAAAACG AAGAAGCCCA AAGTTGTGCA 
TACCATGGTT CAGAGCAATG GGTGAACTAT TGGCTGCATA GTGGGCAGTT GCATGTTAAA 
GGACAATCAG TAAAAATGTC GAAATCTCTG AAAAATACCA TATCTGTGGG TGCAATGTTG 
GAACGTTATA CTGCGGATGA ATTTAGATTA GCTTGTGCAT TATCTAATTA TCGCAATGCT 
ATGGACTACA GTGACACGTT AATGGAAACA TCAAGAAATA CATTGAAAAG ACTTAAATCT 
TTTAAAGCCG ATTGCAATGC TTATTTGAGT GGTAAAAAGC AGGGTGCACA GGTGGAGGCA 
AGTGAGATGC TTGGTGACCT CGAGCGAGCG AAAAATAATA TTGATGCCTG CTATAAAGAT 
GATTTTGATA CATCGTGTAG CATTGGTGTA CTACTTGAAC AGGCAAACGC AGTAAGTCGT 
CAAATTAATG CTGGTACTGC GGAAAACGAA AAGTTATACA CTAGCAGTTG CTTGGATGCC 
GTGGCGGCGG TGCAAAATTT TGTGCAACAA CAACTGCAAA TTTTGGGTTT CAGTCTTGTA 
GAAGAATCTA GAGCACATGT GATGGGTGCA GCAACTGAAT TCGACTTGGA AGGTTTGGTA 
GAGGATTTAT TACAATCAAG GCGCCTAATA CGAGAACGTG CCGTAGCGGG GAAAAATAAG 
GAACTATTTC AAATATGTGA CGAGCTTCGT AACTGCTTAC GGCAACACGG TATTGATATA 
CAAGATCACA ATAAGGGTAC TTCATGGCAA TTTAGTGGAA ATAAACAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004522943.1
CDS	174..1784
Translation	MSRSSYLKRFSFLSLQSRYLCSQHQWKQPSEGRDTGIRIYNCVARKQVPLILSNDAYATWYTCGPTVYDFTHIGHISCYVKLDIIQRILRHHFNVNLVTAMNITDIDDKIIQKSIESGKPWHELSRFYENEFWSVLKQLNVQEPNIKVRVTENIPRIVEFITRIIGKGSAYKGNDGSVYFAVNTCRNYGKLQKTNIDEDPEKPILGVEGKTAPADFALWKAQKCENEPAWPSPWGPGRPGWHIECSTLASMIFGQQLDFHAGGLDLRFPHHENEEAQSCAYHGSEQWVNYWLHSGQLHVKGQSVKMSKSLKNTISVGAMLERYTADEFRLACALSNYRNAMDYSDTLMETSRNTLKRLKSFKADCNAYLSGKKQGAQVEASEMLGDLERAKNNIDACYKDDFDTSCSIGVLLEQANAVSRQINAGTAENEKLYTSSCLDAVAAVQNFVQQQLQILGFSLVEESRAHVMGAATEFDLEGLVEDLLQSRRLIRERAVAGKNKELFQICDELRNCLRQHGIDIQDHNKGTSWQFSGNKQ

Target ORF information:

RefSeq Version	XM_004522886.3
Organism	Ceratitis capitata(Mediterranean fruit fly)
Definition	Ceratitis capitata cysteine--tRNA ligase, mitochondrial (LOC101454443), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004522886.3

ORF Insert Sequence:

ATGTCTCGCT CCAGTTACTT GAAGCGGTTC AGTTTCCTCT CACTACAAAG TAGATACCTT 
TGTAGTCAAC ACCAATGGAA ACAACCGAGC GAAGGGAGAG ATACAGGCAT ACGGATTTAT 
AATTGTGTTG CTCGAAAGCA AGTACCCCTA ATACTTTCCA ACGATGCATA TGCAACTTGG 
TATACATGTG GGCCCACTGT TTATGACTTC ACACATATTG GACACATCAG TTGCTATGTT 
AAATTAGACA TTATACAACG TATTTTGCGC CACCATTTCA ATGTGAATTT AGTAACGGCT 
ATGAATATAA CTGATATAGA TGATAAAATT ATACAGAAAA GTATAGAAAG TGGTAAACCG 
TGGCATGAGT TATCTCGTTT CTACGAAAAC GAATTCTGGT CGGTTCTTAA GCAACTAAAT 
GTGCAAGAGC CAAATATAAA AGTACGCGTC ACTGAGAATA TACCACGAAT TGTCGAATTT 
ATCACGCGCA TCATAGGAAA AGGTAGTGCT TACAAAGGTA ACGATGGCAG TGTATACTTT 
GCCGTCAATA CATGCAGAAA CTATGGTAAA CTGCAAAAGA CAAATATAGA TGAAGACCCG 
GAAAAGCCAA TACTTGGTGT AGAAGGCAAA ACGGCTCCCG CGGATTTTGC TCTATGGAAG 
GCACAAAAAT GTGAAAATGA ACCAGCTTGG CCATCACCTT GGGGCCCAGG ACGTCCAGGT 
TGGCATATTG AATGTAGCAC TTTGGCCAGC ATGATATTTG GACAACAACT GGACTTTCAT 
GCCGGAGGCT TAGACTTACG TTTTCCTCAT CACGAAAACG AAGAAGCCCA AAGTTGTGCA 
TACCATGGTT CAGAGCAATG GGTGAACTAT TGGCTGCATA GTGGGCAGTT GCATGTTAAA 
GGACAATCAG TAAAAATGTC GAAATCTCTG AAAAATACCA TATCTGTGGG TGCAATGTTG 
GAACGTTATA CTGCGGATGA ATTTAGATTA GCTTGTGCAT TATCTAATTA TCGCAATGCT 
ATGGACTACA GTGACACGTT AATGGAAACA TCAAGAAATA CATTGAAAAG ACTTAAATCT 
TTTAAAGCCG ATTGCAATGC TTATTTGAGT GGTAAAAAGC AGGGTGCACA GGTGGAGGCA 
AGTGAGATGC TTGGTGACCT CGAGCGAGCG AAAAATAATA TTGATGCCTG CTATAAAGAT 
GATTTTGATA CATCGTGTAG CATTGGTGTA CTACTTGAAC AGGCAAACGC AGTAAGTCGT 
CAAATTAATG CTGGTACTGC GGAAAACGAA AAGTTATACA CTAGCAGTTG CTTGGATGCC 
GTGGCGGCGG TGCAAAATTT TGTGCAACAA CAACTGCAAA TTTTGGGTTT CAGTCTTGTA 
GAAGAATCTA GAGCACATGT GATGGGTGCA GCAACTGAAT TCGACTTGGA AGGTTTGGTA 
GAGGATTTAT TACAATCAAG GCGCCTAATA CGAGAACGTG CCGTAGCGGG GAAAAATAAG 
GAACTATTTC AAATATGTGA CGAGCTTCGT AACTGCTTAC GGCAACACGG TATTGATATA 
CAAGATCACA ATAAGGGTAC TTCATGGCAA TTTAGTGGAA ATAAACAATA A