AFF3 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014117469.1	XP_013972944.1	AF4/FMR2 family member 3 isoform X2
XM_014117468.2	XP_013972943.1	AF4/FMR2 family member 3 isoform X1
XM_014117468.1	XP_013972943.1	AF4/FMR2 family member 3 isoform X1
XM_005626015.2	XP_005626072.1	AF4/FMR2 family member 3 isoform X4
XM_022424585.1	XP_022280293.1	AF4/FMR2 family member 3 isoform X5
XM_022424586.1	XP_022280294.1	AF4/FMR2 family member 3 isoform X6
XM_005626014.2	XP_005626071.1	AF4/FMR2 family member 3 isoform X3
XM_014117469.2	XP_013972944.1	AF4/FMR2 family member 3 isoform X2
XM_005626014.3	XP_005626071.1	AF4/FMR2 family member 3 isoform X3
XM_005626015.3	XP_005626072.1	AF4/FMR2 family member 3 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe13654	XM_005626014.3 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13655	XM_005626015.3 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe291563	XM_022424585.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe291564	XM_022424586.1 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe38981	XM_014117468.2 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe38982	XM_014117469.2 Latest version!	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OCe38981	XM_014117468.1	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe38982	XM_014117469.1	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13654	XM_005626014.2	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe13655	XM_005626015.2	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

AFF3 ( XM_005626014.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626071.1 Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe13654
Clone ID Related Accession (Same CDS sequence)	XM_005626014.2 , XM_005626014.3
Accession Version	XM_005626014.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACTGCTCTCC ACGCTCTGTC TGACAGCACC 
GTGGTCCAGC AGGCCAACTG CAGAGCCTCT GTGCCTTCCA GCAAGGGCAG CAGCAGTGGC 
AGTAGCAGCA GCGGCAGCAG CTCCTCCAGT GACTCAGAGA GCAGCTCTGG ATCTGACTCG 
GAGACCGAGA GCAGTTCCAG CGAGAGTGAG GGCAGCAAGC CTCCCCACTA CTCCAGCCCC 
GAGGCTGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGACA AATGGCTAAA CAAAGTTAAT 
CCCCACAAGC CGCCTATTCT GATCCAAAAT GAGAGCCACG GGCCAGAGAG CAATCAATAC 
TACGCTCCTG CGAAAGAGGA AGTGCAGGAC TGTGCGAAGC TGGCCGACGT TTGCCAAGCC 
AGCCTGCGAG ACAAGGACAT CAAGAGCGCC TGCAAGGAGG AGCAGAGGCC AAGGACTGCG 
AACAAGGCCC CGGGGAGCAA GGGAGTGAAG CAGAAGTCGC CGCCCGCCGC CGTGGCCGTG 
GCCGTGAGCG CAGCCGCCCA GCCGCCCGCC GCGCCCGGTG CGCCCGCCGC CGAGAGCACA 
CCTGCGCCTG CCCGGAGGGC CGCGGGCAAG AAGCCCACCC GGCGCACCGA CAGGACCGCC 
GCCGGCGACG CCACCAACTG CCACCGGCCC GAGGAGCCCG CGGCTGCCGA CGCGCTGGGG 
GCGAGCGTGG TGGGCCCCCC GGAGCCCGCC AAGACCAGGC CCTGCGGCAA CAGCAGAACG 
AGCCACCGCA AGGAGCTGCG CTCCTCGGTG ACCTGCGAGA AGCGCCGCAC GCGGGGGCTG 
AGCAGGATCG TCCCCAAATC CAAGGAGTTC ATAGACACAG AATCCTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCCGAGCAA GAGGAGTACC CTCTGTCCAA AGCGCAGGCC 
GTGGCCGCCG CCGCCTCCTC GGCGAGTGAC CAGAGGCTGA AGGAGGCTGC GGGCCCCATC 
AGCAGTGGCG GCGGCCCCCG GGCCCCCGTA GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACGCTCGTCC CTTTTGGCCG CAACGAACTT 
CTCTCCCCCT TGAAGGACAG CGATGAGGTC AGGTCTCTCT GGGTCAAAAT TGACCTGACC 
CTGCTGTCCA GGATCCCAGA ACACCTGCCC CAGGAGCCCG GGGTCTTGAA TGCCCCTGCG 
GCCAAGGACG CCGAGAGCAC GCAGCCAAGC CATCCGTCTG ACGCTCCTGC CGAAAAGGCT 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAATGAAG ATGATTACAG GGAGGTCAAG 
AAGGCCCAGG GAGAGAAAGA GAGCTCTTCG CGACTGACCA CCTCTGCCAA TAATACTTTA 
TCTGCAAACC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCACCCAT CTCACCCCTC TCTGATGCAT CTAAACACAA ATACTCCAGC 
GAGGACTTAA CTTCTAGCAG ATCAAATGGC AACTTCACTT CTGCTTCCTC CAACAAAAAG 
CATAAGGCTG AGAGCCAGCT GCAGTCTCAT GCTGGAGACC TCACGAAAGC TGCTCACAAC 
AATTCCGAAA ACGTTCTCCA CAAGCCACGG CCACAAACAG AGCCATGGTC TCCAGGTTCC 
AACGGCCACA GGGATTGCAA GAGGCAGAAA CTTGTCTTCG ATGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAACGGATG AAGCATAAAG CAGATGCAAT GGTGGAAAAG 
TTTGGAAAGG CTTTGAACTA TGCTGAAGCA GCTTTGTCAT TTATTGAGTG TGGAAATGCA 
ATGGAACAAG GCCCAATGGA ATCCAAATCT CCTTATACAA TGTATTCGGA AACAGTAGAG 
CTCATCAGGT ATGCTATGAG ACTAAAAACT CATTCAGGCC CCAATGCCAC ACCAGAGGAC 
AAACAACTGG CTGCATTATG TTACCGCTGC CTGGCTCTCC TGTACTGGCG GATGTTTCGA 
CTCAAAAGGG ACCATGCTGT GAAGTATTCA AAAGCACTTA TTGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGAGCCAGTG GAAAGAGCAC TGGAACCCCA 
TCCCCTATGT CTCCCAACCC CTCCCCAACC AGCTCCGTGG GATCTCAGGG GAGCCTCTCC 
AACGCCAGCG CCCTGTCCCC TTCAACCATC GTCAGCATCC CACAGCGCAT CCACCAGATG 
GCAGCCAATC ACGTCAGCAT CACCAACAGC ATTCTCCACA GCTATGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCAATG ACCTGGATTT GCTCATGGGG 
CCTGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGTCTGCAC 
TGGCTGCGGA ACAGCACCCA CCTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626071.1
CDS	216..3965
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_005626014.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626014.2

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACTGCTCTCC ACGCTCTGTC TGACAGCACC 
GTGGTCCAGC AGGCCAACTG CAGAGCCTCT GTGCCTTCCA GCAAGGGCAG CAGCAGTGGC 
AGTAGCAGCA GCGGCAGCAG CTCCTCCAGT GACTCAGAGA GCAGCTCTGG ATCTGACTCG 
GAGACCGAGA GCAGTTCCAG CGAGAGTGAG GGCAGCAAGC CTCCCCACTA CTCCAGCCCC 
GAGGCTGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGACA AATGGCTAAA CAAAGTTAAT 
CCCCACAAGC CGCCTATTCT GATCCAAAAT GAGAGCCACG GGCCAGAGAG CAATCAATAC 
TACGCTCCTG CGAAAGAGGA AGTGCAGGAC TGTGCGAAGC TGGCCGACGT TTGCCAAGCC 
AGCCTGCGAG ACAAGGACAT CAAGAGCGCC TGCAAGGAGG AGCAGAGGCC AAGGACTGCG 
AACAAGGCCC CGGGGAGCAA GGGAGTGAAG CAGAAGTCGC CGCCCGCCGC CGTGGCCGTG 
GCCGTGAGCG CAGCCGCCCA GCCGCCCGCC GCGCCCGGTG CGCCCGCCGC CGAGAGCACA 
CCTGCGCCTG CCCGGAGGGC CGCGGGCAAG AAGCCCACCC GGCGCACCGA CAGGACCGCC 
GCCGGCGACG CCACCAACTG CCACCGGCCC GAGGAGCCCG CGGCTGCCGA CGCGCTGGGG 
GCGAGCGTGG TGGGCCCCCC GGAGCCCGCC AAGACCAGGC CCTGCGGCAA CAGCAGAACG 
AGCCACCGCA AGGAGCTGCG CTCCTCGGTG ACCTGCGAGA AGCGCCGCAC GCGGGGGCTG 
AGCAGGATCG TCCCCAAATC CAAGGAGTTC ATAGACACAG AATCCTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCCGAGCAA GAGGAGTACC CTCTGTCCAA AGCGCAGGCC 
GTGGCCGCCG CCGCCTCCTC GGCGAGTGAC CAGAGGCTGA AGGAGGCTGC GGGCCCCATC 
AGCAGTGGCG GCGGCCCCCG GGCCCCCGTA GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACGCTCGTCC CTTTTGGCCG CAACGAACTT 
CTCTCCCCCT TGAAGGACAG CGATGAGGTC AGGTCTCTCT GGGTCAAAAT TGACCTGACC 
CTGCTGTCCA GGATCCCAGA ACACCTGCCC CAGGAGCCCG GGGTCTTGAA TGCCCCTGCG 
GCCAAGGACG CCGAGAGCAC GCAGCCAAGC CATCCGTCTG ACGCTCCTGC CGAAAAGGCT 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAATGAAG ATGATTACAG GGAGGTCAAG 
AAGGCCCAGG GAGAGAAAGA GAGCTCTTCG CGACTGACCA CCTCTGCCAA TAATACTTTA 
TCTGCAAACC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCACCCAT CTCACCCCTC TCTGATGCAT CTAAACACAA ATACTCCAGC 
GAGGACTTAA CTTCTAGCAG ATCAAATGGC AACTTCACTT CTGCTTCCTC CAACAAAAAG 
CATAAGGCTG AGAGCCAGCT GCAGTCTCAT GCTGGAGACC TCACGAAAGC TGCTCACAAC 
AATTCCGAAA ACGTTCTCCA CAAGCCACGG CCACAAACAG AGCCATGGTC TCCAGGTTCC 
AACGGCCACA GGGATTGCAA GAGGCAGAAA CTTGTCTTCG ATGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAACGGATG AAGCATAAAG CAGATGCAAT GGTGGAAAAG 
TTTGGAAAGG CTTTGAACTA TGCTGAAGCA GCTTTGTCAT TTATTGAGTG TGGAAATGCA 
ATGGAACAAG GCCCAATGGA ATCCAAATCT CCTTATACAA TGTATTCGGA AACAGTAGAG 
CTCATCAGGT ATGCTATGAG ACTAAAAACT CATTCAGGCC CCAATGCCAC ACCAGAGGAC 
AAACAACTGG CTGCATTATG TTACCGCTGC CTGGCTCTCC TGTACTGGCG GATGTTTCGA 
CTCAAAAGGG ACCATGCTGT GAAGTATTCA AAAGCACTTA TTGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGAGCCAGTG GAAAGAGCAC TGGAACCCCA 
TCCCCTATGT CTCCCAACCC CTCCCCAACC AGCTCCGTGG GATCTCAGGG GAGCCTCTCC 
AACGCCAGCG CCCTGTCCCC TTCAACCATC GTCAGCATCC CACAGCGCAT CCACCAGATG 
GCAGCCAATC ACGTCAGCAT CACCAACAGC ATTCTCCACA GCTATGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCAATG ACCTGGATTT GCTCATGGGG 
CCTGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGTCTGCAC 
TGGCTGCGGA ACAGCACCCA CCTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_005626014.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626071.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe13654
Clone ID Related Accession (Same CDS sequence)	XM_005626014.2 , XM_005626014.3
Accession Version	XM_005626014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACTGCTCTCC ACGCTCTGTC TGACAGCACC 
GTGGTCCAGC AGGCCAACTG CAGAGCCTCT GTGCCTTCCA GCAAGGGCAG CAGCAGTGGC 
AGTAGCAGCA GCGGCAGCAG CTCCTCCAGT GACTCAGAGA GCAGCTCTGG ATCTGACTCG 
GAGACCGAGA GCAGTTCCAG CGAGAGTGAG GGCAGCAAGC CTCCCCACTA CTCCAGCCCC 
GAGGCTGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGACA AATGGCTAAA CAAAGTTAAT 
CCCCACAAGC CGCCTATTCT GATCCAAAAT GAGAGCCACG GGCCAGAGAG CAATCAATAC 
TACGCTCCTG CGAAAGAGGA AGTGCAGGAC TGTGCGAAGC TGGCCGACGT TTGCCAAGCC 
AGCCTGCGAG ACAAGGACAT CAAGAGCGCC TGCAAGGAGG AGCAGAGGCC AAGGACTGCG 
AACAAGGCCC CGGGGAGCAA GGGAGTGAAG CAGAAGTCGC CGCCCGCCGC CGTGGCCGTG 
GCCGTGAGCG CAGCCGCCCA GCCGCCCGCC GCGCCCGGTG CGCCCGCCGC CGAGAGCACA 
CCTGCGCCTG CCCGGAGGGC CGCGGGCAAG AAGCCCACCC GGCGCACCGA CAGGACCGCC 
GCCGGCGACG CCACCAACTG CCACCGGCCC GAGGAGCCCG CGGCTGCCGA CGCGCTGGGG 
GCGAGCGTGG TGGGCCCCCC GGAGCCCGCC AAGACCAGGC CCTGCGGCAA CAGCAGAACG 
AGCCACCGCA AGGAGCTGCG CTCCTCGGTG ACCTGCGAGA AGCGCCGCAC GCGGGGGCTG 
AGCAGGATCG TCCCCAAATC CAAGGAGTTC ATAGACACAG AATCCTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCCGAGCAA GAGGAGTACC CTCTGTCCAA AGCGCAGGCC 
GTGGCCGCCG CCGCCTCCTC GGCGAGTGAC CAGAGGCTGA AGGAGGCTGC GGGCCCCATC 
AGCAGTGGCG GCGGCCCCCG GGCCCCCGTA GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACGCTCGTCC CTTTTGGCCG CAACGAACTT 
CTCTCCCCCT TGAAGGACAG CGATGAGGTC AGGTCTCTCT GGGTCAAAAT TGACCTGACC 
CTGCTGTCCA GGATCCCAGA ACACCTGCCC CAGGAGCCCG GGGTCTTGAA TGCCCCTGCG 
GCCAAGGACG CCGAGAGCAC GCAGCCAAGC CATCCGTCTG ACGCTCCTGC CGAAAAGGCT 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAATGAAG ATGATTACAG GGAGGTCAAG 
AAGGCCCAGG GAGAGAAAGA GAGCTCTTCG CGACTGACCA CCTCTGCCAA TAATACTTTA 
TCTGCAAACC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCACCCAT CTCACCCCTC TCTGATGCAT CTAAACACAA ATACTCCAGC 
GAGGACTTAA CTTCTAGCAG ATCAAATGGC AACTTCACTT CTGCTTCCTC CAACAAAAAG 
CATAAGGCTG AGAGCCAGCT GCAGTCTCAT GCTGGAGACC TCACGAAAGC TGCTCACAAC 
AATTCCGAAA ACGTTCTCCA CAAGCCACGG CCACAAACAG AGCCATGGTC TCCAGGTTCC 
AACGGCCACA GGGATTGCAA GAGGCAGAAA CTTGTCTTCG ATGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAACGGATG AAGCATAAAG CAGATGCAAT GGTGGAAAAG 
TTTGGAAAGG CTTTGAACTA TGCTGAAGCA GCTTTGTCAT TTATTGAGTG TGGAAATGCA 
ATGGAACAAG GCCCAATGGA ATCCAAATCT CCTTATACAA TGTATTCGGA AACAGTAGAG 
CTCATCAGGT ATGCTATGAG ACTAAAAACT CATTCAGGCC CCAATGCCAC ACCAGAGGAC 
AAACAACTGG CTGCATTATG TTACCGCTGC CTGGCTCTCC TGTACTGGCG GATGTTTCGA 
CTCAAAAGGG ACCATGCTGT GAAGTATTCA AAAGCACTTA TTGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGAGCCAGTG GAAAGAGCAC TGGAACCCCA 
TCCCCTATGT CTCCCAACCC CTCCCCAACC AGCTCCGTGG GATCTCAGGG GAGCCTCTCC 
AACGCCAGCG CCCTGTCCCC TTCAACCATC GTCAGCATCC CACAGCGCAT CCACCAGATG 
GCAGCCAATC ACGTCAGCAT CACCAACAGC ATTCTCCACA GCTATGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCAATG ACCTGGATTT GCTCATGGGG 
CCTGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGTCTGCAC 
TGGCTGCGGA ACAGCACCCA CCTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626071.1
CDS	210..3959
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_005626014.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626014.3

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACTGCTCTCC ACGCTCTGTC TGACAGCACC 
GTGGTCCAGC AGGCCAACTG CAGAGCCTCT GTGCCTTCCA GCAAGGGCAG CAGCAGTGGC 
AGTAGCAGCA GCGGCAGCAG CTCCTCCAGT GACTCAGAGA GCAGCTCTGG ATCTGACTCG 
GAGACCGAGA GCAGTTCCAG CGAGAGTGAG GGCAGCAAGC CTCCCCACTA CTCCAGCCCC 
GAGGCTGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGACA AATGGCTAAA CAAAGTTAAT 
CCCCACAAGC CGCCTATTCT GATCCAAAAT GAGAGCCACG GGCCAGAGAG CAATCAATAC 
TACGCTCCTG CGAAAGAGGA AGTGCAGGAC TGTGCGAAGC TGGCCGACGT TTGCCAAGCC 
AGCCTGCGAG ACAAGGACAT CAAGAGCGCC TGCAAGGAGG AGCAGAGGCC AAGGACTGCG 
AACAAGGCCC CGGGGAGCAA GGGAGTGAAG CAGAAGTCGC CGCCCGCCGC CGTGGCCGTG 
GCCGTGAGCG CAGCCGCCCA GCCGCCCGCC GCGCCCGGTG CGCCCGCCGC CGAGAGCACA 
CCTGCGCCTG CCCGGAGGGC CGCGGGCAAG AAGCCCACCC GGCGCACCGA CAGGACCGCC 
GCCGGCGACG CCACCAACTG CCACCGGCCC GAGGAGCCCG CGGCTGCCGA CGCGCTGGGG 
GCGAGCGTGG TGGGCCCCCC GGAGCCCGCC AAGACCAGGC CCTGCGGCAA CAGCAGAACG 
AGCCACCGCA AGGAGCTGCG CTCCTCGGTG ACCTGCGAGA AGCGCCGCAC GCGGGGGCTG 
AGCAGGATCG TCCCCAAATC CAAGGAGTTC ATAGACACAG AATCCTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCCGAGCAA GAGGAGTACC CTCTGTCCAA AGCGCAGGCC 
GTGGCCGCCG CCGCCTCCTC GGCGAGTGAC CAGAGGCTGA AGGAGGCTGC GGGCCCCATC 
AGCAGTGGCG GCGGCCCCCG GGCCCCCGTA GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACGCTCGTCC CTTTTGGCCG CAACGAACTT 
CTCTCCCCCT TGAAGGACAG CGATGAGGTC AGGTCTCTCT GGGTCAAAAT TGACCTGACC 
CTGCTGTCCA GGATCCCAGA ACACCTGCCC CAGGAGCCCG GGGTCTTGAA TGCCCCTGCG 
GCCAAGGACG CCGAGAGCAC GCAGCCAAGC CATCCGTCTG ACGCTCCTGC CGAAAAGGCT 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAATGAAG ATGATTACAG GGAGGTCAAG 
AAGGCCCAGG GAGAGAAAGA GAGCTCTTCG CGACTGACCA CCTCTGCCAA TAATACTTTA 
TCTGCAAACC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCACCCAT CTCACCCCTC TCTGATGCAT CTAAACACAA ATACTCCAGC 
GAGGACTTAA CTTCTAGCAG ATCAAATGGC AACTTCACTT CTGCTTCCTC CAACAAAAAG 
CATAAGGCTG AGAGCCAGCT GCAGTCTCAT GCTGGAGACC TCACGAAAGC TGCTCACAAC 
AATTCCGAAA ACGTTCTCCA CAAGCCACGG CCACAAACAG AGCCATGGTC TCCAGGTTCC 
AACGGCCACA GGGATTGCAA GAGGCAGAAA CTTGTCTTCG ATGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAACGGATG AAGCATAAAG CAGATGCAAT GGTGGAAAAG 
TTTGGAAAGG CTTTGAACTA TGCTGAAGCA GCTTTGTCAT TTATTGAGTG TGGAAATGCA 
ATGGAACAAG GCCCAATGGA ATCCAAATCT CCTTATACAA TGTATTCGGA AACAGTAGAG 
CTCATCAGGT ATGCTATGAG ACTAAAAACT CATTCAGGCC CCAATGCCAC ACCAGAGGAC 
AAACAACTGG CTGCATTATG TTACCGCTGC CTGGCTCTCC TGTACTGGCG GATGTTTCGA 
CTCAAAAGGG ACCATGCTGT GAAGTATTCA AAAGCACTTA TTGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGAGCCAGTG GAAAGAGCAC TGGAACCCCA 
TCCCCTATGT CTCCCAACCC CTCCCCAACC AGCTCCGTGG GATCTCAGGG GAGCCTCTCC 
AACGCCAGCG CCCTGTCCCC TTCAACCATC GTCAGCATCC CACAGCGCAT CCACCAGATG 
GCAGCCAATC ACGTCAGCAT CACCAACAGC ATTCTCCACA GCTATGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCAATG ACCTGGATTT GCTCATGGGG 
CCTGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGTCTGCAC 
TGGCTGCGGA ACAGCACCCA CCTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_005626015.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626072.1 Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X4, mRNA.

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CloneID	OCe13655
Clone ID Related Accession (Same CDS sequence)	XM_005626015.2 , XM_005626015.3
Accession Version	XM_005626015.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGGCAGC CCAGAGAACT GCTCTCCACG CTCTGTCTGA CAGCACCGTG 
GTCCAGCAGG CCAACTGCAG AGCCTCTGTG CCTTCCAGCA AGGGCAGCAG CAGTGGCAGT 
AGCAGCAGCG GCAGCAGCTC CTCCAGTGAC TCAGAGAGCA GCTCTGGATC TGACTCGGAG 
ACCGAGAGCA GTTCCAGCGA GAGTGAGGGC AGCAAGCCTC CCCACTACTC CAGCCCCGAG 
GCTGAACCAG CGTCCTCTAA CAAGTGGCAG CTGGACAAAT GGCTAAACAA AGTTAATCCC 
CACAAGCCGC CTATTCTGAT CCAAAATGAG AGCCACGGGC CAGAGAGCAA TCAATACTAC 
GCTCCTGCGA AAGAGGAAGT GCAGGACTGT GCGAAGCTGG CCGACGTTTG CCAAGCCAGC 
CTGCGAGACA AGGACATCAA GAGCGCCTGC AAGGAGGAGC AGAGGCCAAG GACTGCGAAC 
AAGGCCCCGG GGAGCAAGGG AGTGAAGCAG AAGTCGCCGC CCGCCGCCGT GGCCGTGGCC 
GTGAGCGCAG CCGCCCAGCC GCCCGCCGCG CCCGGTGCGC CCGCCGCCGA GAGCACACCT 
GCGCCTGCCC GGAGGGCCGC GGGCAAGAAG CCCACCCGGC GCACCGACAG GACCGCCGCC 
GGCGACGCCA CCAACTGCCA CCGGCCCGAG GAGCCCGCGG CTGCCGACGC GCTGGGGGCG 
AGCGTGGTGG GCCCCCCGGA GCCCGCCAAG ACCAGGCCCT GCGGCAACAG CAGAACGAGC 
CACCGCAAGG AGCTGCGCTC CTCGGTGACC TGCGAGAAGC GCCGCACGCG GGGGCTGAGC 
AGGATCGTCC CCAAATCCAA GGAGTTCATA GACACAGAAT CCTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC CGAGCAAGAG GAGTACCCTC TGTCCAAAGC GCAGGCCGTG 
GCCGCCGCCG CCTCCTCGGC GAGTGACCAG AGGCTGAAGG AGGCTGCGGG CCCCATCAGC 
AGTGGCGGCG GCCCCCGGGC CCCCGTAGGC TCCATCAACG CCAGGACCAC CAGCGACATT 
GCCAAGGAGC TGGAGGAGCA GTTCTACACG CTCGTCCCTT TTGGCCGCAA CGAACTTCTC 
TCCCCCTTGA AGGACAGCGA TGAGGTCAGG TCTCTCTGGG TCAAAATTGA CCTGACCCTG 
CTGTCCAGGA TCCCAGAACA CCTGCCCCAG GAGCCCGGGG TCTTGAATGC CCCTGCGGCC 
AAGGACGCCG AGAGCACGCA GCCAAGCCAT CCGTCTGACG CTCCTGCCGA AAAGGCTTTG 
CCAAAATCCA AGAGGAAACG CAAGTGTGAC AATGAAGATG ATTACAGGGA GGTCAAGAAG 
GCCCAGGGAG AGAAAGAGAG CTCTTCGCGA CTGACCACCT CTGCCAATAA TACTTTATCT 
GCAAACCATT GCAGCATGAA CATCAACAGC TTGGCAATAC CAATAAATAA AAATGAAAAA 
GTGCTCCGGT CACCCATCTC ACCCCTCTCT GATGCATCTA AACACAAATA CTCCAGCGAG 
GACTTAACTT CTAGCAGATC AAATGGCAAC TTCACTTCTG CTTCCTCCAA CAAAAAGCAT 
AAGGCTGAGA GCCAGCTGCA GTCTCATGCT GGAGACCTCA CGAAAGCTGC TCACAACAAT 
TCCGAAAACG TTCTCCACAA GCCACGGCCA CAAACAGAGC CATGGTCTCC AGGTTCCAAC 
GGCCACAGGG ATTGCAAGAG GCAGAAACTT GTCTTCGATG ATATGCCACG CAGTGCAGAT 
TATTTTATGC AAGAAGCTAA ACGGATGAAG CATAAAGCAG ATGCAATGGT GGAAAAGTTT 
GGAAAGGCTT TGAACTATGC TGAAGCAGCT TTGTCATTTA TTGAGTGTGG AAATGCAATG 
GAACAAGGCC CAATGGAATC CAAATCTCCT TATACAATGT ATTCGGAAAC AGTAGAGCTC 
ATCAGGTATG CTATGAGACT AAAAACTCAT TCAGGCCCCA ATGCCACACC AGAGGACAAA 
CAACTGGCTG CATTATGTTA CCGCTGCCTG GCTCTCCTGT ACTGGCGGAT GTTTCGACTC 
AAAAGGGACC ATGCTGTGAA GTATTCAAAA GCACTTATTG ACTATTTCAA GAATTCATCT 
AAAGCTGCCC AAGCCCCATC TCCATGGGGA GCCAGTGGAA AGAGCACTGG AACCCCATCC 
CCTATGTCTC CCAACCCCTC CCCAACCAGC TCCGTGGGAT CTCAGGGGAG CCTCTCCAAC 
GCCAGCGCCC TGTCCCCTTC AACCATCGTC AGCATCCCAC AGCGCATCCA CCAGATGGCA 
GCCAATCACG TCAGCATCAC CAACAGCATT CTCCACAGCT ATGACTACTG GGAGATGGCA 
GACAACCTGG CCAAGGAAAA CAGAGAATTC TTCAATGACC TGGATTTGCT CATGGGGCCT 
GTCACCCTGC ACAGCAGCAT GGAGCACCTG GTCCAGTACT CCCAGCAGGG TCTGCACTGG 
CTGCGGAACA GCACCCACCT GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626072.1
CDS	216..3962
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_005626015.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626015.2

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGGCAGC CCAGAGAACT GCTCTCCACG CTCTGTCTGA CAGCACCGTG 
GTCCAGCAGG CCAACTGCAG AGCCTCTGTG CCTTCCAGCA AGGGCAGCAG CAGTGGCAGT 
AGCAGCAGCG GCAGCAGCTC CTCCAGTGAC TCAGAGAGCA GCTCTGGATC TGACTCGGAG 
ACCGAGAGCA GTTCCAGCGA GAGTGAGGGC AGCAAGCCTC CCCACTACTC CAGCCCCGAG 
GCTGAACCAG CGTCCTCTAA CAAGTGGCAG CTGGACAAAT GGCTAAACAA AGTTAATCCC 
CACAAGCCGC CTATTCTGAT CCAAAATGAG AGCCACGGGC CAGAGAGCAA TCAATACTAC 
GCTCCTGCGA AAGAGGAAGT GCAGGACTGT GCGAAGCTGG CCGACGTTTG CCAAGCCAGC 
CTGCGAGACA AGGACATCAA GAGCGCCTGC AAGGAGGAGC AGAGGCCAAG GACTGCGAAC 
AAGGCCCCGG GGAGCAAGGG AGTGAAGCAG AAGTCGCCGC CCGCCGCCGT GGCCGTGGCC 
GTGAGCGCAG CCGCCCAGCC GCCCGCCGCG CCCGGTGCGC CCGCCGCCGA GAGCACACCT 
GCGCCTGCCC GGAGGGCCGC GGGCAAGAAG CCCACCCGGC GCACCGACAG GACCGCCGCC 
GGCGACGCCA CCAACTGCCA CCGGCCCGAG GAGCCCGCGG CTGCCGACGC GCTGGGGGCG 
AGCGTGGTGG GCCCCCCGGA GCCCGCCAAG ACCAGGCCCT GCGGCAACAG CAGAACGAGC 
CACCGCAAGG AGCTGCGCTC CTCGGTGACC TGCGAGAAGC GCCGCACGCG GGGGCTGAGC 
AGGATCGTCC CCAAATCCAA GGAGTTCATA GACACAGAAT CCTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC CGAGCAAGAG GAGTACCCTC TGTCCAAAGC GCAGGCCGTG 
GCCGCCGCCG CCTCCTCGGC GAGTGACCAG AGGCTGAAGG AGGCTGCGGG CCCCATCAGC 
AGTGGCGGCG GCCCCCGGGC CCCCGTAGGC TCCATCAACG CCAGGACCAC CAGCGACATT 
GCCAAGGAGC TGGAGGAGCA GTTCTACACG CTCGTCCCTT TTGGCCGCAA CGAACTTCTC 
TCCCCCTTGA AGGACAGCGA TGAGGTCAGG TCTCTCTGGG TCAAAATTGA CCTGACCCTG 
CTGTCCAGGA TCCCAGAACA CCTGCCCCAG GAGCCCGGGG TCTTGAATGC CCCTGCGGCC 
AAGGACGCCG AGAGCACGCA GCCAAGCCAT CCGTCTGACG CTCCTGCCGA AAAGGCTTTG 
CCAAAATCCA AGAGGAAACG CAAGTGTGAC AATGAAGATG ATTACAGGGA GGTCAAGAAG 
GCCCAGGGAG AGAAAGAGAG CTCTTCGCGA CTGACCACCT CTGCCAATAA TACTTTATCT 
GCAAACCATT GCAGCATGAA CATCAACAGC TTGGCAATAC CAATAAATAA AAATGAAAAA 
GTGCTCCGGT CACCCATCTC ACCCCTCTCT GATGCATCTA AACACAAATA CTCCAGCGAG 
GACTTAACTT CTAGCAGATC AAATGGCAAC TTCACTTCTG CTTCCTCCAA CAAAAAGCAT 
AAGGCTGAGA GCCAGCTGCA GTCTCATGCT GGAGACCTCA CGAAAGCTGC TCACAACAAT 
TCCGAAAACG TTCTCCACAA GCCACGGCCA CAAACAGAGC CATGGTCTCC AGGTTCCAAC 
GGCCACAGGG ATTGCAAGAG GCAGAAACTT GTCTTCGATG ATATGCCACG CAGTGCAGAT 
TATTTTATGC AAGAAGCTAA ACGGATGAAG CATAAAGCAG ATGCAATGGT GGAAAAGTTT 
GGAAAGGCTT TGAACTATGC TGAAGCAGCT TTGTCATTTA TTGAGTGTGG AAATGCAATG 
GAACAAGGCC CAATGGAATC CAAATCTCCT TATACAATGT ATTCGGAAAC AGTAGAGCTC 
ATCAGGTATG CTATGAGACT AAAAACTCAT TCAGGCCCCA ATGCCACACC AGAGGACAAA 
CAACTGGCTG CATTATGTTA CCGCTGCCTG GCTCTCCTGT ACTGGCGGAT GTTTCGACTC 
AAAAGGGACC ATGCTGTGAA GTATTCAAAA GCACTTATTG ACTATTTCAA GAATTCATCT 
AAAGCTGCCC AAGCCCCATC TCCATGGGGA GCCAGTGGAA AGAGCACTGG AACCCCATCC 
CCTATGTCTC CCAACCCCTC CCCAACCAGC TCCGTGGGAT CTCAGGGGAG CCTCTCCAAC 
GCCAGCGCCC TGTCCCCTTC AACCATCGTC AGCATCCCAC AGCGCATCCA CCAGATGGCA 
GCCAATCACG TCAGCATCAC CAACAGCATT CTCCACAGCT ATGACTACTG GGAGATGGCA 
GACAACCTGG CCAAGGAAAA CAGAGAATTC TTCAATGACC TGGATTTGCT CATGGGGCCT 
GTCACCCTGC ACAGCAGCAT GGAGCACCTG GTCCAGTACT CCCAGCAGGG TCTGCACTGG 
CTGCGGAACA GCACCCACCT GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_005626015.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626072.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe13655
Clone ID Related Accession (Same CDS sequence)	XM_005626015.2 , XM_005626015.3
Accession Version	XM_005626015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626015.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGGCAGC CCAGAGAACT GCTCTCCACG CTCTGTCTGA CAGCACCGTG 
GTCCAGCAGG CCAACTGCAG AGCCTCTGTG CCTTCCAGCA AGGGCAGCAG CAGTGGCAGT 
AGCAGCAGCG GCAGCAGCTC CTCCAGTGAC TCAGAGAGCA GCTCTGGATC TGACTCGGAG 
ACCGAGAGCA GTTCCAGCGA GAGTGAGGGC AGCAAGCCTC CCCACTACTC CAGCCCCGAG 
GCTGAACCAG CGTCCTCTAA CAAGTGGCAG CTGGACAAAT GGCTAAACAA AGTTAATCCC 
CACAAGCCGC CTATTCTGAT CCAAAATGAG AGCCACGGGC CAGAGAGCAA TCAATACTAC 
GCTCCTGCGA AAGAGGAAGT GCAGGACTGT GCGAAGCTGG CCGACGTTTG CCAAGCCAGC 
CTGCGAGACA AGGACATCAA GAGCGCCTGC AAGGAGGAGC AGAGGCCAAG GACTGCGAAC 
AAGGCCCCGG GGAGCAAGGG AGTGAAGCAG AAGTCGCCGC CCGCCGCCGT GGCCGTGGCC 
GTGAGCGCAG CCGCCCAGCC GCCCGCCGCG CCCGGTGCGC CCGCCGCCGA GAGCACACCT 
GCGCCTGCCC GGAGGGCCGC GGGCAAGAAG CCCACCCGGC GCACCGACAG GACCGCCGCC 
GGCGACGCCA CCAACTGCCA CCGGCCCGAG GAGCCCGCGG CTGCCGACGC GCTGGGGGCG 
AGCGTGGTGG GCCCCCCGGA GCCCGCCAAG ACCAGGCCCT GCGGCAACAG CAGAACGAGC 
CACCGCAAGG AGCTGCGCTC CTCGGTGACC TGCGAGAAGC GCCGCACGCG GGGGCTGAGC 
AGGATCGTCC CCAAATCCAA GGAGTTCATA GACACAGAAT CCTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC CGAGCAAGAG GAGTACCCTC TGTCCAAAGC GCAGGCCGTG 
GCCGCCGCCG CCTCCTCGGC GAGTGACCAG AGGCTGAAGG AGGCTGCGGG CCCCATCAGC 
AGTGGCGGCG GCCCCCGGGC CCCCGTAGGC TCCATCAACG CCAGGACCAC CAGCGACATT 
GCCAAGGAGC TGGAGGAGCA GTTCTACACG CTCGTCCCTT TTGGCCGCAA CGAACTTCTC 
TCCCCCTTGA AGGACAGCGA TGAGGTCAGG TCTCTCTGGG TCAAAATTGA CCTGACCCTG 
CTGTCCAGGA TCCCAGAACA CCTGCCCCAG GAGCCCGGGG TCTTGAATGC CCCTGCGGCC 
AAGGACGCCG AGAGCACGCA GCCAAGCCAT CCGTCTGACG CTCCTGCCGA AAAGGCTTTG 
CCAAAATCCA AGAGGAAACG CAAGTGTGAC AATGAAGATG ATTACAGGGA GGTCAAGAAG 
GCCCAGGGAG AGAAAGAGAG CTCTTCGCGA CTGACCACCT CTGCCAATAA TACTTTATCT 
GCAAACCATT GCAGCATGAA CATCAACAGC TTGGCAATAC CAATAAATAA AAATGAAAAA 
GTGCTCCGGT CACCCATCTC ACCCCTCTCT GATGCATCTA AACACAAATA CTCCAGCGAG 
GACTTAACTT CTAGCAGATC AAATGGCAAC TTCACTTCTG CTTCCTCCAA CAAAAAGCAT 
AAGGCTGAGA GCCAGCTGCA GTCTCATGCT GGAGACCTCA CGAAAGCTGC TCACAACAAT 
TCCGAAAACG TTCTCCACAA GCCACGGCCA CAAACAGAGC CATGGTCTCC AGGTTCCAAC 
GGCCACAGGG ATTGCAAGAG GCAGAAACTT GTCTTCGATG ATATGCCACG CAGTGCAGAT 
TATTTTATGC AAGAAGCTAA ACGGATGAAG CATAAAGCAG ATGCAATGGT GGAAAAGTTT 
GGAAAGGCTT TGAACTATGC TGAAGCAGCT TTGTCATTTA TTGAGTGTGG AAATGCAATG 
GAACAAGGCC CAATGGAATC CAAATCTCCT TATACAATGT ATTCGGAAAC AGTAGAGCTC 
ATCAGGTATG CTATGAGACT AAAAACTCAT TCAGGCCCCA ATGCCACACC AGAGGACAAA 
CAACTGGCTG CATTATGTTA CCGCTGCCTG GCTCTCCTGT ACTGGCGGAT GTTTCGACTC 
AAAAGGGACC ATGCTGTGAA GTATTCAAAA GCACTTATTG ACTATTTCAA GAATTCATCT 
AAAGCTGCCC AAGCCCCATC TCCATGGGGA GCCAGTGGAA AGAGCACTGG AACCCCATCC 
CCTATGTCTC CCAACCCCTC CCCAACCAGC TCCGTGGGAT CTCAGGGGAG CCTCTCCAAC 
GCCAGCGCCC TGTCCCCTTC AACCATCGTC AGCATCCCAC AGCGCATCCA CCAGATGGCA 
GCCAATCACG TCAGCATCAC CAACAGCATT CTCCACAGCT ATGACTACTG GGAGATGGCA 
GACAACCTGG CCAAGGAAAA CAGAGAATTC TTCAATGACC TGGATTTGCT CATGGGGCCT 
GTCACCCTGC ACAGCAGCAT GGAGCACCTG GTCCAGTACT CCCAGCAGGG TCTGCACTGG 
CTGCGGAACA GCACCCACCT GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626072.1
CDS	210..3956
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_005626015.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626015.3

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCAATGTTA GAAGATGACC TTAAGCTAAG TAGTGATGAA 
GAGGAGAATG AACAGGCAGC CCAGAGAACT GCTCTCCACG CTCTGTCTGA CAGCACCGTG 
GTCCAGCAGG CCAACTGCAG AGCCTCTGTG CCTTCCAGCA AGGGCAGCAG CAGTGGCAGT 
AGCAGCAGCG GCAGCAGCTC CTCCAGTGAC TCAGAGAGCA GCTCTGGATC TGACTCGGAG 
ACCGAGAGCA GTTCCAGCGA GAGTGAGGGC AGCAAGCCTC CCCACTACTC CAGCCCCGAG 
GCTGAACCAG CGTCCTCTAA CAAGTGGCAG CTGGACAAAT GGCTAAACAA AGTTAATCCC 
CACAAGCCGC CTATTCTGAT CCAAAATGAG AGCCACGGGC CAGAGAGCAA TCAATACTAC 
GCTCCTGCGA AAGAGGAAGT GCAGGACTGT GCGAAGCTGG CCGACGTTTG CCAAGCCAGC 
CTGCGAGACA AGGACATCAA GAGCGCCTGC AAGGAGGAGC AGAGGCCAAG GACTGCGAAC 
AAGGCCCCGG GGAGCAAGGG AGTGAAGCAG AAGTCGCCGC CCGCCGCCGT GGCCGTGGCC 
GTGAGCGCAG CCGCCCAGCC GCCCGCCGCG CCCGGTGCGC CCGCCGCCGA GAGCACACCT 
GCGCCTGCCC GGAGGGCCGC GGGCAAGAAG CCCACCCGGC GCACCGACAG GACCGCCGCC 
GGCGACGCCA CCAACTGCCA CCGGCCCGAG GAGCCCGCGG CTGCCGACGC GCTGGGGGCG 
AGCGTGGTGG GCCCCCCGGA GCCCGCCAAG ACCAGGCCCT GCGGCAACAG CAGAACGAGC 
CACCGCAAGG AGCTGCGCTC CTCGGTGACC TGCGAGAAGC GCCGCACGCG GGGGCTGAGC 
AGGATCGTCC CCAAATCCAA GGAGTTCATA GACACAGAAT CCTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC CGAGCAAGAG GAGTACCCTC TGTCCAAAGC GCAGGCCGTG 
GCCGCCGCCG CCTCCTCGGC GAGTGACCAG AGGCTGAAGG AGGCTGCGGG CCCCATCAGC 
AGTGGCGGCG GCCCCCGGGC CCCCGTAGGC TCCATCAACG CCAGGACCAC CAGCGACATT 
GCCAAGGAGC TGGAGGAGCA GTTCTACACG CTCGTCCCTT TTGGCCGCAA CGAACTTCTC 
TCCCCCTTGA AGGACAGCGA TGAGGTCAGG TCTCTCTGGG TCAAAATTGA CCTGACCCTG 
CTGTCCAGGA TCCCAGAACA CCTGCCCCAG GAGCCCGGGG TCTTGAATGC CCCTGCGGCC 
AAGGACGCCG AGAGCACGCA GCCAAGCCAT CCGTCTGACG CTCCTGCCGA AAAGGCTTTG 
CCAAAATCCA AGAGGAAACG CAAGTGTGAC AATGAAGATG ATTACAGGGA GGTCAAGAAG 
GCCCAGGGAG AGAAAGAGAG CTCTTCGCGA CTGACCACCT CTGCCAATAA TACTTTATCT 
GCAAACCATT GCAGCATGAA CATCAACAGC TTGGCAATAC CAATAAATAA AAATGAAAAA 
GTGCTCCGGT CACCCATCTC ACCCCTCTCT GATGCATCTA AACACAAATA CTCCAGCGAG 
GACTTAACTT CTAGCAGATC AAATGGCAAC TTCACTTCTG CTTCCTCCAA CAAAAAGCAT 
AAGGCTGAGA GCCAGCTGCA GTCTCATGCT GGAGACCTCA CGAAAGCTGC TCACAACAAT 
TCCGAAAACG TTCTCCACAA GCCACGGCCA CAAACAGAGC CATGGTCTCC AGGTTCCAAC 
GGCCACAGGG ATTGCAAGAG GCAGAAACTT GTCTTCGATG ATATGCCACG CAGTGCAGAT 
TATTTTATGC AAGAAGCTAA ACGGATGAAG CATAAAGCAG ATGCAATGGT GGAAAAGTTT 
GGAAAGGCTT TGAACTATGC TGAAGCAGCT TTGTCATTTA TTGAGTGTGG AAATGCAATG 
GAACAAGGCC CAATGGAATC CAAATCTCCT TATACAATGT ATTCGGAAAC AGTAGAGCTC 
ATCAGGTATG CTATGAGACT AAAAACTCAT TCAGGCCCCA ATGCCACACC AGAGGACAAA 
CAACTGGCTG CATTATGTTA CCGCTGCCTG GCTCTCCTGT ACTGGCGGAT GTTTCGACTC 
AAAAGGGACC ATGCTGTGAA GTATTCAAAA GCACTTATTG ACTATTTCAA GAATTCATCT 
AAAGCTGCCC AAGCCCCATC TCCATGGGGA GCCAGTGGAA AGAGCACTGG AACCCCATCC 
CCTATGTCTC CCAACCCCTC CCCAACCAGC TCCGTGGGAT CTCAGGGGAG CCTCTCCAAC 
GCCAGCGCCC TGTCCCCTTC AACCATCGTC AGCATCCCAC AGCGCATCCA CCAGATGGCA 
GCCAATCACG TCAGCATCAC CAACAGCATT CTCCACAGCT ATGACTACTG GGAGATGGCA 
GACAACCTGG CCAAGGAAAA CAGAGAATTC TTCAATGACC TGGATTTGCT CATGGGGCCT 
GTCACCCTGC ACAGCAGCAT GGAGCACCTG GTCCAGTACT CCCAGCAGGG TCTGCACTGG 
CTGCGGAACA GCACCCACCT GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_022424585.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280293.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe291563
Clone ID Related Accession (Same CDS sequence)	XM_022424585.1
Accession Version	XM_022424585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG CGTCTATGAA 
CCAGATAGAA ATGTGTTACG AAGGAAGGAA CGAGAGAGAA GAAATCAGGA AACACAGCAG 
GATGATGGCA CATTTAATTC GAGTTACTCC CTCTTCAGTG AACCTTATAA GACTAACAAG 
GGGGATGAGC TCTCCAACCG GATCCAGAAT ACTTTAGGCA ATTACGATGA AATGAAAGAC 
TTTTTAACTG ATCGATCTAA TCAGAGTCAT CTTGTTGGCG TTCCCAAACC TGGGGTTCCT 
CAGGCTCCCG TGAACAAGAT CGATGAACAT TTTGCTGCAG ATTCAAGAGC CCAGGCCCAG 
CCCTCATCTG TCTGTAGCAC TGCTTCTTCC ACACCAGCAG CTGTCCCCGT GCAGCAGAGT 
AAGAGAGGCA CCATGGGCTG GCAGAAGGCC GGGCATCCGC CATCAGATGG CCAACAGAGA 
GCAACACAAC AAGGCTCTCT CAGGACCTTG CTTGGAGATG GTGTTGGCAG ACAGCAGCCA 
CGAGCCAAAC AAGTGTGCAA TGTAGAGGTG GGTCTTCAGA CCCAGGAAAG GCCACCTGCT 
ATGGCAGCCA AGCATAGCAG CAGCGGACAC TGTGTTCAGA ACTTTCCTCC ATCCCTGGCT 
TCAAAACCCA GCCTGGTCCA GCAGAAACCA ACTGCATATG TGCGGCCGAT GGATGGCCAA 
GATCAAGCTC CCGACGAGTC TCCAAAGCTT AAGTCGTCCA CAGAAACGGG TGTGCACTGC 
ACCTCATACA GGGGAGTCCC GGCCACCAAG CCAGAGTCTG CCAGAGCCAA GGCCAAACTT 
TCCAAGTTAA GCATCCCCAA ACAAGGAGAG GAGAGTAGAT CTGGAGACAC AAACAGCTGT 
GTTGAAGAAA TAATTCGGGA GATGACCTGG CTCCCACCAC TTTCTGCTAT TCCAGCACCT 
GGAAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGTTTCCTCC 
GGACACAATA ATCCAAAGAA AGGTGATGCT GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AATACATCCT CTCTGGAATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACAGAGA 
GGAATGTTAG AAGATGACCT TAAGCTAAGT AGTGATGAAG AGGAGAATGA ACAGCAGGCA 
GCCCAGAGAA CTGCTCTCCA CGCTCTGTCT GACAGCACCG TGGTCCAGCA GGCCAACTGC 
AGAGCCTCTG TGCCTTCCAG CAAGGGCAGC AGCAGTGGCA GTAGCAGCAG CGGCAGCAGC 
TCCTCCAGTG ACTCAGAGAG CAGCTCTGGA TCTGACTCGG AGACCGAGAG CAGTTCCAGC 
GAGAGTGAGG GCAGCAAGCC TCCCCACTAC TCCAGCCCCG AGGCTGAACC AGCGTCCTCT 
AACAAGTGGC AGCTGGACAA ATGGCTAAAC AAAGTTAATC CCCACAAGCC GCCTATTCTG 
ATCCAAAATG AGAGCCACGG GCCAGAGAGC AATCAATACT ACGCTCCTGC GAAAGAGGAA 
GTGCAGGACT GTGCGAAGCT GGCCGACGTT TGCCAAGCCA GCCTGCGAGA CAAGGACATC 
AAGAGCGCCT GCAAGGAGGA GCAGAGGCCA AGGACTGCGA ACAAGGCCCC GGGGAGCAAG 
GGAGTGAAGC AGAAGTCGCC GCCCGCCGCC GTGGCCGTGG CCGTGAGCGC AGCCGCCCAG 
CCGCCCGCCG CGCCCGGTGC GCCCGCCGCC GAGAGCACAC CTGCGCCTGC CCGGAGGGCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAC AGGACCGCCG CCGGCGACGC CACCAACTGC 
CACCGGCCCG AGGAGCCCGC GGCTGCCGAC GCGCTGGGGG CGAGCGTGGT GGGCCCCCCG 
GAGCCCGCCA AGACCAGGCC CTGCGGCAAC AGCAGAACGA GCCACCGCAA GGAGCTGCGC 
TCCTCGGTGA CCTGCGAGAA GCGCCGCACG CGGGGGCTGA GCAGGATCGT CCCCAAATCC 
AAGGAGTTCA TAGACACAGA ATCCTCCTCT TCCTCCTCCT CCTCGGACTC GGACCTGGAG 
TCCGAGCAAG AGGAGTACCC TCTGTCCAAA GCGCAGGCCG TGGCCGCCGC CGCCTCCTCG 
GCGAGTGACC AGAGGCTGAA GGAGGCTGCG GGCCCCATCA GCAGTGGCGG CGGCCCCCGG 
GCCCCCGTAG GCTCCATCAA CGCCAGGACC ACCAGCGACA TTGCCAAGGA GCTGGAGGAG 
CAGTTCTACA CGCTCGTCCC TTTTGGCCGC AACGAACTTC TCTCCCCCTT GAAGGACAGC 
GATGAGGTCA GGTCTCTCTG GGTCAAAATT GACCTGACCC TGCTGTCCAG GATCCCAGAA 
CACCTGCCCC AGGAGCCCGG GGTCTTGAAT GCCCCTGCGG CCAAGGACGC CGAGAGCACG 
CAGCCAAGCC ATCCGTCTGA CGCTCCTGCC GAAAAGGCTT TGCCAAAATC CAAGAGGAAA 
CGCAAGTGTG ACAATGAAGA TGATTACAGG GAGGTCAAGA AGGCCCAGGG AGAGAAAGAG 
AGCTCTTCGC GACTGACCAC CTCTGCCAAT AATACTTTAT CTGCAAACCA TTGCAGCATG 
AACATCAACA GCTTGGCAAT ACCAATAAAT AAAAATGAAA AAGTGCTCCG GTCACCCATC 
TCACCCCTCT CTGATGCATC TAAACACAAA TACTCCAGCG AGGACTTAAC TTCTAGCAGA 
TCAAATGGCA ACTTCACTTC TGCTTCCTCC AACAAAAAGC ATAAGGCTGA GAGCCAGCTG 
CAGTCTCATG CTGGAGACCT CACGAAAGCT GCTCACAACA ATTCCGAAAA CGTTCTCCAC 
AAGCCACGGC CACAAACAGA GCCATGGTCT CCAGGTTCCA ACGGCCACAG GGATTGCAAG 
AGGCAGAAAC TTGTCTTCGA TGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAACGGATGA AGCATAAAGC AGATGCAATG GTGGAAAAGT TTGGAAAGGC TTTGAACTAT 
GCTGAAGCAG CTTTGTCATT TATTGAGTGT GGAAATGCAA TGGAACAAGG CCCAATGGAA 
TCCAAATCTC CTTATACAAT GTATTCGGAA ACAGTAGAGC TCATCAGGTA TGCTATGAGA 
CTAAAAACTC ATTCAGGCCC CAATGCCACA CCAGAGGACA AACAACTGGC TGCATTATGT 
TACCGCTGCC TGGCTCTCCT GTACTGGCGG ATGTTTCGAC TCAAAAGGGA CCATGCTGTG 
AAGTATTCAA AAGCACTTAT TGACTATTTC AAGAATTCAT CTAAAGCTGC CCAAGCCCCA 
TCTCCATGGG GAGCCAGTGG AAAGAGCACT GGAACCCCAT CCCCTATGTC TCCCAACCCC 
TCCCCAACCA GCTCCGTGGG ATCTCAGGGG AGCCTCTCCA ACGCCAGCGC CCTGTCCCCT 
TCAACCATCG TCAGCATCCC ACAGCGCATC CACCAGATGG CAGCCAATCA CGTCAGCATC 
ACCAACAGCA TTCTCCACAG CTATGACTAC TGGGAGATGG CAGACAACCT GGCCAAGGAA 
AACAGAGAAT TCTTCAATGA CCTGGATTTG CTCATGGGGC CTGTCACCCT GCACAGCAGC 
ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGTCTGCACT GGCTGCGGAA CAGCACCCAC 
CTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280293.1
CDS	211..3939
Translation	MNICWNEHINCTLFCLCVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_022424585.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424585.1

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG CGTCTATGAA 
CCAGATAGAA ATGTGTTACG AAGGAAGGAA CGAGAGAGAA GAAATCAGGA AACACAGCAG 
GATGATGGCA CATTTAATTC GAGTTACTCC CTCTTCAGTG AACCTTATAA GACTAACAAG 
GGGGATGAGC TCTCCAACCG GATCCAGAAT ACTTTAGGCA ATTACGATGA AATGAAAGAC 
TTTTTAACTG ATCGATCTAA TCAGAGTCAT CTTGTTGGCG TTCCCAAACC TGGGGTTCCT 
CAGGCTCCCG TGAACAAGAT CGATGAACAT TTTGCTGCAG ATTCAAGAGC CCAGGCCCAG 
CCCTCATCTG TCTGTAGCAC TGCTTCTTCC ACACCAGCAG CTGTCCCCGT GCAGCAGAGT 
AAGAGAGGCA CCATGGGCTG GCAGAAGGCC GGGCATCCGC CATCAGATGG CCAACAGAGA 
GCAACACAAC AAGGCTCTCT CAGGACCTTG CTTGGAGATG GTGTTGGCAG ACAGCAGCCA 
CGAGCCAAAC AAGTGTGCAA TGTAGAGGTG GGTCTTCAGA CCCAGGAAAG GCCACCTGCT 
ATGGCAGCCA AGCATAGCAG CAGCGGACAC TGTGTTCAGA ACTTTCCTCC ATCCCTGGCT 
TCAAAACCCA GCCTGGTCCA GCAGAAACCA ACTGCATATG TGCGGCCGAT GGATGGCCAA 
GATCAAGCTC CCGACGAGTC TCCAAAGCTT AAGTCGTCCA CAGAAACGGG TGTGCACTGC 
ACCTCATACA GGGGAGTCCC GGCCACCAAG CCAGAGTCTG CCAGAGCCAA GGCCAAACTT 
TCCAAGTTAA GCATCCCCAA ACAAGGAGAG GAGAGTAGAT CTGGAGACAC AAACAGCTGT 
GTTGAAGAAA TAATTCGGGA GATGACCTGG CTCCCACCAC TTTCTGCTAT TCCAGCACCT 
GGAAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGTTTCCTCC 
GGACACAATA ATCCAAAGAA AGGTGATGCT GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AATACATCCT CTCTGGAATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACAGAGA 
GGAATGTTAG AAGATGACCT TAAGCTAAGT AGTGATGAAG AGGAGAATGA ACAGCAGGCA 
GCCCAGAGAA CTGCTCTCCA CGCTCTGTCT GACAGCACCG TGGTCCAGCA GGCCAACTGC 
AGAGCCTCTG TGCCTTCCAG CAAGGGCAGC AGCAGTGGCA GTAGCAGCAG CGGCAGCAGC 
TCCTCCAGTG ACTCAGAGAG CAGCTCTGGA TCTGACTCGG AGACCGAGAG CAGTTCCAGC 
GAGAGTGAGG GCAGCAAGCC TCCCCACTAC TCCAGCCCCG AGGCTGAACC AGCGTCCTCT 
AACAAGTGGC AGCTGGACAA ATGGCTAAAC AAAGTTAATC CCCACAAGCC GCCTATTCTG 
ATCCAAAATG AGAGCCACGG GCCAGAGAGC AATCAATACT ACGCTCCTGC GAAAGAGGAA 
GTGCAGGACT GTGCGAAGCT GGCCGACGTT TGCCAAGCCA GCCTGCGAGA CAAGGACATC 
AAGAGCGCCT GCAAGGAGGA GCAGAGGCCA AGGACTGCGA ACAAGGCCCC GGGGAGCAAG 
GGAGTGAAGC AGAAGTCGCC GCCCGCCGCC GTGGCCGTGG CCGTGAGCGC AGCCGCCCAG 
CCGCCCGCCG CGCCCGGTGC GCCCGCCGCC GAGAGCACAC CTGCGCCTGC CCGGAGGGCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAC AGGACCGCCG CCGGCGACGC CACCAACTGC 
CACCGGCCCG AGGAGCCCGC GGCTGCCGAC GCGCTGGGGG CGAGCGTGGT GGGCCCCCCG 
GAGCCCGCCA AGACCAGGCC CTGCGGCAAC AGCAGAACGA GCCACCGCAA GGAGCTGCGC 
TCCTCGGTGA CCTGCGAGAA GCGCCGCACG CGGGGGCTGA GCAGGATCGT CCCCAAATCC 
AAGGAGTTCA TAGACACAGA ATCCTCCTCT TCCTCCTCCT CCTCGGACTC GGACCTGGAG 
TCCGAGCAAG AGGAGTACCC TCTGTCCAAA GCGCAGGCCG TGGCCGCCGC CGCCTCCTCG 
GCGAGTGACC AGAGGCTGAA GGAGGCTGCG GGCCCCATCA GCAGTGGCGG CGGCCCCCGG 
GCCCCCGTAG GCTCCATCAA CGCCAGGACC ACCAGCGACA TTGCCAAGGA GCTGGAGGAG 
CAGTTCTACA CGCTCGTCCC TTTTGGCCGC AACGAACTTC TCTCCCCCTT GAAGGACAGC 
GATGAGGTCA GGTCTCTCTG GGTCAAAATT GACCTGACCC TGCTGTCCAG GATCCCAGAA 
CACCTGCCCC AGGAGCCCGG GGTCTTGAAT GCCCCTGCGG CCAAGGACGC CGAGAGCACG 
CAGCCAAGCC ATCCGTCTGA CGCTCCTGCC GAAAAGGCTT TGCCAAAATC CAAGAGGAAA 
CGCAAGTGTG ACAATGAAGA TGATTACAGG GAGGTCAAGA AGGCCCAGGG AGAGAAAGAG 
AGCTCTTCGC GACTGACCAC CTCTGCCAAT AATACTTTAT CTGCAAACCA TTGCAGCATG 
AACATCAACA GCTTGGCAAT ACCAATAAAT AAAAATGAAA AAGTGCTCCG GTCACCCATC 
TCACCCCTCT CTGATGCATC TAAACACAAA TACTCCAGCG AGGACTTAAC TTCTAGCAGA 
TCAAATGGCA ACTTCACTTC TGCTTCCTCC AACAAAAAGC ATAAGGCTGA GAGCCAGCTG 
CAGTCTCATG CTGGAGACCT CACGAAAGCT GCTCACAACA ATTCCGAAAA CGTTCTCCAC 
AAGCCACGGC CACAAACAGA GCCATGGTCT CCAGGTTCCA ACGGCCACAG GGATTGCAAG 
AGGCAGAAAC TTGTCTTCGA TGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAACGGATGA AGCATAAAGC AGATGCAATG GTGGAAAAGT TTGGAAAGGC TTTGAACTAT 
GCTGAAGCAG CTTTGTCATT TATTGAGTGT GGAAATGCAA TGGAACAAGG CCCAATGGAA 
TCCAAATCTC CTTATACAAT GTATTCGGAA ACAGTAGAGC TCATCAGGTA TGCTATGAGA 
CTAAAAACTC ATTCAGGCCC CAATGCCACA CCAGAGGACA AACAACTGGC TGCATTATGT 
TACCGCTGCC TGGCTCTCCT GTACTGGCGG ATGTTTCGAC TCAAAAGGGA CCATGCTGTG 
AAGTATTCAA AAGCACTTAT TGACTATTTC AAGAATTCAT CTAAAGCTGC CCAAGCCCCA 
TCTCCATGGG GAGCCAGTGG AAAGAGCACT GGAACCCCAT CCCCTATGTC TCCCAACCCC 
TCCCCAACCA GCTCCGTGGG ATCTCAGGGG AGCCTCTCCA ACGCCAGCGC CCTGTCCCCT 
TCAACCATCG TCAGCATCCC ACAGCGCATC CACCAGATGG CAGCCAATCA CGTCAGCATC 
ACCAACAGCA TTCTCCACAG CTATGACTAC TGGGAGATGG CAGACAACCT GGCCAAGGAA 
AACAGAGAAT TCTTCAATGA CCTGGATTTG CTCATGGGGC CTGTCACCCT GCACAGCAGC 
ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGTCTGCACT GGCTGCGGAA CAGCACCCAC 
CTGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_022424586.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280294.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.

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CloneID	OCe291564
Clone ID Related Accession (Same CDS sequence)	XM_022424586.1
Accession Version	XM_022424586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGACT TTTTAACTGA TCGATCTAAT CAGAGTCATC TTGTTGGCGT TCCCAAACCT 
GGGGTTCCTC AGGCTCCCGT GAACAAGATC GATGAACATT TTGCTGCAGA TTCAAGAGCC 
CAGGCCCAGC CCTCATCTGT CTGTAGCACT GCTTCTTCCA CACCAGCAGC TGTCCCCGTG 
CAGCAGAGTA AGAGAGGCAC CATGGGCTGG CAGAAGGCCG GGCATCCGCC ATCAGATGGC 
CAACAGAGAG CAACACAACA AGGCTCTCTC AGGACCTTGC TTGGAGATGG TGTTGGCAGA 
CAGCAGCCAC GAGCCAAACA AGTGTGCAAT GTAGAGGTGG GTCTTCAGAC CCAGGAAAGG 
CCACCTGCTA TGGCAGCCAA GCATAGCAGC AGCGGACACT GTGTTCAGAA CTTTCCTCCA 
TCCCTGGCTT CAAAACCCAG CCTGGTCCAG CAGAAACCAA CTGCATATGT GCGGCCGATG 
GATGGCCAAG ATCAAGCTCC CGACGAGTCT CCAAAGCTTA AGTCGTCCAC AGAAACGGGT 
GTGCACTGCA CCTCATACAG GGGAGTCCCG GCCACCAAGC CAGAGTCTGC CAGAGCCAAG 
GCCAAACTTT CCAAGTTAAG CATCCCCAAA CAAGGAGAGG AGAGTAGATC TGGAGACACA 
AACAGCTGTG TTGAAGAAAT AATTCGGGAG ATGACCTGGC TCCCACCACT TTCTGCTATT 
CCAGCACCTG GAAAAGTGGA ACCAAGCAAA TTTCCATTTC CAAATAAGGA CTCTCAGCTT 
GTTTCCTCCG GACACAATAA TCCAAAGAAA GGTGATGCTG AGCCAGAGAG TCCAGACAAT 
GGCACATCGA ATACATCCTC TCTGGAATGG TTATCCCCAT TTACAGATAA GGGAAATGAT 
GCACAGAGAG GAATGTTAGA AGATGACCTT AAGCTAAGTA GTGATGAAGA GGAGAATGAA 
CAGCAGGCAG CCCAGAGAAC TGCTCTCCAC GCTCTGTCTG ACAGCACCGT GGTCCAGCAG 
GCCAACTGCA GAGCCTCTGT GCCTTCCAGC AAGGGCAGCA GCAGTGGCAG TAGCAGCAGC 
GGCAGCAGCT CCTCCAGTGA CTCAGAGAGC AGCTCTGGAT CTGACTCGGA GACCGAGAGC 
AGTTCCAGCG AGAGTGAGGG CAGCAAGCCT CCCCACTACT CCAGCCCCGA GGCTGAACCA 
GCGTCCTCTA ACAAGTGGCA GCTGGACAAA TGGCTAAACA AAGTTAATCC CCACAAGCCG 
CCTATTCTGA TCCAAAATGA GAGCCACGGG CCAGAGAGCA ATCAATACTA CGCTCCTGCG 
AAAGAGGAAG TGCAGGACTG TGCGAAGCTG GCCGACGTTT GCCAAGCCAG CCTGCGAGAC 
AAGGACATCA AGAGCGCCTG CAAGGAGGAG CAGAGGCCAA GGACTGCGAA CAAGGCCCCG 
GGGAGCAAGG GAGTGAAGCA GAAGTCGCCG CCCGCCGCCG TGGCCGTGGC CGTGAGCGCA 
GCCGCCCAGC CGCCCGCCGC GCCCGGTGCG CCCGCCGCCG AGAGCACACC TGCGCCTGCC 
CGGAGGGCCG CGGGCAAGAA GCCCACCCGG CGCACCGACA GGACCGCCGC CGGCGACGCC 
ACCAACTGCC ACCGGCCCGA GGAGCCCGCG GCTGCCGACG CGCTGGGGGC GAGCGTGGTG 
GGCCCCCCGG AGCCCGCCAA GACCAGGCCC TGCGGCAACA GCAGAACGAG CCACCGCAAG 
GAGCTGCGCT CCTCGGTGAC CTGCGAGAAG CGCCGCACGC GGGGGCTGAG CAGGATCGTC 
CCCAAATCCA AGGAGTTCAT AGACACAGAA TCCTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CCGAGCAAGA GGAGTACCCT CTGTCCAAAG CGCAGGCCGT GGCCGCCGCC 
GCCTCCTCGG CGAGTGACCA GAGGCTGAAG GAGGCTGCGG GCCCCATCAG CAGTGGCGGC 
GGCCCCCGGG CCCCCGTAGG CTCCATCAAC GCCAGGACCA CCAGCGACAT TGCCAAGGAG 
CTGGAGGAGC AGTTCTACAC GCTCGTCCCT TTTGGCCGCA ACGAACTTCT CTCCCCCTTG 
AAGGACAGCG ATGAGGTCAG GTCTCTCTGG GTCAAAATTG ACCTGACCCT GCTGTCCAGG 
ATCCCAGAAC ACCTGCCCCA GGAGCCCGGG GTCTTGAATG CCCCTGCGGC CAAGGACGCC 
GAGAGCACGC AGCCAAGCCA TCCGTCTGAC GCTCCTGCCG AAAAGGCTTT GCCAAAATCC 
AAGAGGAAAC GCAAGTGTGA CAATGAAGAT GATTACAGGG AGGTCAAGAA GGCCCAGGGA 
GAGAAAGAGA GCTCTTCGCG ACTGACCACC TCTGCCAATA ATACTTTATC TGCAAACCAT 
TGCAGCATGA ACATCAACAG CTTGGCAATA CCAATAAATA AAAATGAAAA AGTGCTCCGG 
TCACCCATCT CACCCCTCTC TGATGCATCT AAACACAAAT ACTCCAGCGA GGACTTAACT 
TCTAGCAGAT CAAATGGCAA CTTCACTTCT GCTTCCTCCA ACAAAAAGCA TAAGGCTGAG 
AGCCAGCTGC AGTCTCATGC TGGAGACCTC ACGAAAGCTG CTCACAACAA TTCCGAAAAC 
GTTCTCCACA AGCCACGGCC ACAAACAGAG CCATGGTCTC CAGGTTCCAA CGGCCACAGG 
GATTGCAAGA GGCAGAAACT TGTCTTCGAT GATATGCCAC GCAGTGCAGA TTATTTTATG 
CAAGAAGCTA AACGGATGAA GCATAAAGCA GATGCAATGG TGGAAAAGTT TGGAAAGGCT 
TTGAACTATG CTGAAGCAGC TTTGTCATTT ATTGAGTGTG GAAATGCAAT GGAACAAGGC 
CCAATGGAAT CCAAATCTCC TTATACAATG TATTCGGAAA CAGTAGAGCT CATCAGGTAT 
GCTATGAGAC TAAAAACTCA TTCAGGCCCC AATGCCACAC CAGAGGACAA ACAACTGGCT 
GCATTATGTT ACCGCTGCCT GGCTCTCCTG TACTGGCGGA TGTTTCGACT CAAAAGGGAC 
CATGCTGTGA AGTATTCAAA AGCACTTATT GACTATTTCA AGAATTCATC TAAAGCTGCC 
CAAGCCCCAT CTCCATGGGG AGCCAGTGGA AAGAGCACTG GAACCCCATC CCCTATGTCT 
CCCAACCCCT CCCCAACCAG CTCCGTGGGA TCTCAGGGGA GCCTCTCCAA CGCCAGCGCC 
CTGTCCCCTT CAACCATCGT CAGCATCCCA CAGCGCATCC ACCAGATGGC AGCCAATCAC 
GTCAGCATCA CCAACAGCAT TCTCCACAGC TATGACTACT GGGAGATGGC AGACAACCTG 
GCCAAGGAAA ACAGAGAATT CTTCAATGAC CTGGATTTGC TCATGGGGCC TGTCACCCTG 
CACAGCAGCA TGGAGCACCT GGTCCAGTAC TCCCAGCAGG GTCTGCACTG GCTGCGGAAC 
AGCACCCACC TGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280294.1
CDS	358..3855
Translation	MKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_022424586.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424586.1

ORF Insert Sequence:

ATGAAAGACT TTTTAACTGA TCGATCTAAT CAGAGTCATC TTGTTGGCGT TCCCAAACCT 
GGGGTTCCTC AGGCTCCCGT GAACAAGATC GATGAACATT TTGCTGCAGA TTCAAGAGCC 
CAGGCCCAGC CCTCATCTGT CTGTAGCACT GCTTCTTCCA CACCAGCAGC TGTCCCCGTG 
CAGCAGAGTA AGAGAGGCAC CATGGGCTGG CAGAAGGCCG GGCATCCGCC ATCAGATGGC 
CAACAGAGAG CAACACAACA AGGCTCTCTC AGGACCTTGC TTGGAGATGG TGTTGGCAGA 
CAGCAGCCAC GAGCCAAACA AGTGTGCAAT GTAGAGGTGG GTCTTCAGAC CCAGGAAAGG 
CCACCTGCTA TGGCAGCCAA GCATAGCAGC AGCGGACACT GTGTTCAGAA CTTTCCTCCA 
TCCCTGGCTT CAAAACCCAG CCTGGTCCAG CAGAAACCAA CTGCATATGT GCGGCCGATG 
GATGGCCAAG ATCAAGCTCC CGACGAGTCT CCAAAGCTTA AGTCGTCCAC AGAAACGGGT 
GTGCACTGCA CCTCATACAG GGGAGTCCCG GCCACCAAGC CAGAGTCTGC CAGAGCCAAG 
GCCAAACTTT CCAAGTTAAG CATCCCCAAA CAAGGAGAGG AGAGTAGATC TGGAGACACA 
AACAGCTGTG TTGAAGAAAT AATTCGGGAG ATGACCTGGC TCCCACCACT TTCTGCTATT 
CCAGCACCTG GAAAAGTGGA ACCAAGCAAA TTTCCATTTC CAAATAAGGA CTCTCAGCTT 
GTTTCCTCCG GACACAATAA TCCAAAGAAA GGTGATGCTG AGCCAGAGAG TCCAGACAAT 
GGCACATCGA ATACATCCTC TCTGGAATGG TTATCCCCAT TTACAGATAA GGGAAATGAT 
GCACAGAGAG GAATGTTAGA AGATGACCTT AAGCTAAGTA GTGATGAAGA GGAGAATGAA 
CAGCAGGCAG CCCAGAGAAC TGCTCTCCAC GCTCTGTCTG ACAGCACCGT GGTCCAGCAG 
GCCAACTGCA GAGCCTCTGT GCCTTCCAGC AAGGGCAGCA GCAGTGGCAG TAGCAGCAGC 
GGCAGCAGCT CCTCCAGTGA CTCAGAGAGC AGCTCTGGAT CTGACTCGGA GACCGAGAGC 
AGTTCCAGCG AGAGTGAGGG CAGCAAGCCT CCCCACTACT CCAGCCCCGA GGCTGAACCA 
GCGTCCTCTA ACAAGTGGCA GCTGGACAAA TGGCTAAACA AAGTTAATCC CCACAAGCCG 
CCTATTCTGA TCCAAAATGA GAGCCACGGG CCAGAGAGCA ATCAATACTA CGCTCCTGCG 
AAAGAGGAAG TGCAGGACTG TGCGAAGCTG GCCGACGTTT GCCAAGCCAG CCTGCGAGAC 
AAGGACATCA AGAGCGCCTG CAAGGAGGAG CAGAGGCCAA GGACTGCGAA CAAGGCCCCG 
GGGAGCAAGG GAGTGAAGCA GAAGTCGCCG CCCGCCGCCG TGGCCGTGGC CGTGAGCGCA 
GCCGCCCAGC CGCCCGCCGC GCCCGGTGCG CCCGCCGCCG AGAGCACACC TGCGCCTGCC 
CGGAGGGCCG CGGGCAAGAA GCCCACCCGG CGCACCGACA GGACCGCCGC CGGCGACGCC 
ACCAACTGCC ACCGGCCCGA GGAGCCCGCG GCTGCCGACG CGCTGGGGGC GAGCGTGGTG 
GGCCCCCCGG AGCCCGCCAA GACCAGGCCC TGCGGCAACA GCAGAACGAG CCACCGCAAG 
GAGCTGCGCT CCTCGGTGAC CTGCGAGAAG CGCCGCACGC GGGGGCTGAG CAGGATCGTC 
CCCAAATCCA AGGAGTTCAT AGACACAGAA TCCTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CCGAGCAAGA GGAGTACCCT CTGTCCAAAG CGCAGGCCGT GGCCGCCGCC 
GCCTCCTCGG CGAGTGACCA GAGGCTGAAG GAGGCTGCGG GCCCCATCAG CAGTGGCGGC 
GGCCCCCGGG CCCCCGTAGG CTCCATCAAC GCCAGGACCA CCAGCGACAT TGCCAAGGAG 
CTGGAGGAGC AGTTCTACAC GCTCGTCCCT TTTGGCCGCA ACGAACTTCT CTCCCCCTTG 
AAGGACAGCG ATGAGGTCAG GTCTCTCTGG GTCAAAATTG ACCTGACCCT GCTGTCCAGG 
ATCCCAGAAC ACCTGCCCCA GGAGCCCGGG GTCTTGAATG CCCCTGCGGC CAAGGACGCC 
GAGAGCACGC AGCCAAGCCA TCCGTCTGAC GCTCCTGCCG AAAAGGCTTT GCCAAAATCC 
AAGAGGAAAC GCAAGTGTGA CAATGAAGAT GATTACAGGG AGGTCAAGAA GGCCCAGGGA 
GAGAAAGAGA GCTCTTCGCG ACTGACCACC TCTGCCAATA ATACTTTATC TGCAAACCAT 
TGCAGCATGA ACATCAACAG CTTGGCAATA CCAATAAATA AAAATGAAAA AGTGCTCCGG 
TCACCCATCT CACCCCTCTC TGATGCATCT AAACACAAAT ACTCCAGCGA GGACTTAACT 
TCTAGCAGAT CAAATGGCAA CTTCACTTCT GCTTCCTCCA ACAAAAAGCA TAAGGCTGAG 
AGCCAGCTGC AGTCTCATGC TGGAGACCTC ACGAAAGCTG CTCACAACAA TTCCGAAAAC 
GTTCTCCACA AGCCACGGCC ACAAACAGAG CCATGGTCTC CAGGTTCCAA CGGCCACAGG 
GATTGCAAGA GGCAGAAACT TGTCTTCGAT GATATGCCAC GCAGTGCAGA TTATTTTATG 
CAAGAAGCTA AACGGATGAA GCATAAAGCA GATGCAATGG TGGAAAAGTT TGGAAAGGCT 
TTGAACTATG CTGAAGCAGC TTTGTCATTT ATTGAGTGTG GAAATGCAAT GGAACAAGGC 
CCAATGGAAT CCAAATCTCC TTATACAATG TATTCGGAAA CAGTAGAGCT CATCAGGTAT 
GCTATGAGAC TAAAAACTCA TTCAGGCCCC AATGCCACAC CAGAGGACAA ACAACTGGCT 
GCATTATGTT ACCGCTGCCT GGCTCTCCTG TACTGGCGGA TGTTTCGACT CAAAAGGGAC 
CATGCTGTGA AGTATTCAAA AGCACTTATT GACTATTTCA AGAATTCATC TAAAGCTGCC 
CAAGCCCCAT CTCCATGGGG AGCCAGTGGA AAGAGCACTG GAACCCCATC CCCTATGTCT 
CCCAACCCCT CCCCAACCAG CTCCGTGGGA TCTCAGGGGA GCCTCTCCAA CGCCAGCGCC 
CTGTCCCCTT CAACCATCGT CAGCATCCCA CAGCGCATCC ACCAGATGGC AGCCAATCAC 
GTCAGCATCA CCAACAGCAT TCTCCACAGC TATGACTACT GGGAGATGGC AGACAACCTG 
GCCAAGGAAA ACAGAGAATT CTTCAATGAC CTGGATTTGC TCATGGGGCC TGTCACCCTG 
CACAGCAGCA TGGAGCACCT GGTCCAGTAC TCCCAGCAGG GTCTGCACTG GCTGCGGAAC 
AGCACCCACC TGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_014117468.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972943.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

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CloneID	OCe38981
Clone ID Related Accession (Same CDS sequence)	XM_014117468.2 , XM_014117468.1
Accession Version	XM_014117468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGC AGGCAGCCCA GAGAACTGCT CTCCACGCTC TGTCTGACAG CACCGTGGTC 
CAGCAGGCCA ACTGCAGAGC CTCTGTGCCT TCCAGCAAGG GCAGCAGCAG TGGCAGTAGC 
AGCAGCGGCA GCAGCTCCTC CAGTGACTCA GAGAGCAGCT CTGGATCTGA CTCGGAGACC 
GAGAGCAGTT CCAGCGAGAG TGAGGGCAGC AAGCCTCCCC ACTACTCCAG CCCCGAGGCT 
GAACCAGCGT CCTCTAACAA GTGGCAGCTG GACAAATGGC TAAACAAAGT TAATCCCCAC 
AAGCCGCCTA TTCTGATCCA AAATGAGAGC CACGGGCCAG AGAGCAATCA ATACTACGCT 
CCTGCGAAAG AGGAAGTGCA GGACTGTGCG AAGCTGGCCG ACGTTTGCCA AGCCAGCCTG 
CGAGACAAGG ACATCAAGAG CGCCTGCAAG GAGGAGCAGA GGCCAAGGAC TGCGAACAAG 
GCCCCGGGGA GCAAGGGAGT GAAGCAGAAG TCGCCGCCCG CCGCCGTGGC CGTGGCCGTG 
AGCGCAGCCG CCCAGCCGCC CGCCGCGCCC GGTGCGCCCG CCGCCGAGAG CACACCTGCG 
CCTGCCCGGA GGGCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGACAGGAC CGCCGCCGGC 
GACGCCACCA ACTGCCACCG GCCCGAGGAG CCCGCGGCTG CCGACGCGCT GGGGGCGAGC 
GTGGTGGGCC CCCCGGAGCC CGCCAAGACC AGGCCCTGCG GCAACAGCAG AACGAGCCAC 
CGCAAGGAGC TGCGCTCCTC GGTGACCTGC GAGAAGCGCC GCACGCGGGG GCTGAGCAGG 
ATCGTCCCCA AATCCAAGGA GTTCATAGAC ACAGAATCCT CCTCTTCCTC CTCCTCCTCG 
GACTCGGACC TGGAGTCCGA GCAAGAGGAG TACCCTCTGT CCAAAGCGCA GGCCGTGGCC 
GCCGCCGCCT CCTCGGCGAG TGACCAGAGG CTGAAGGAGG CTGCGGGCCC CATCAGCAGT 
GGCGGCGGCC CCCGGGCCCC CGTAGGCTCC ATCAACGCCA GGACCACCAG CGACATTGCC 
AAGGAGCTGG AGGAGCAGTT CTACACGCTC GTCCCTTTTG GCCGCAACGA ACTTCTCTCC 
CCCTTGAAGG ACAGCGATGA GGTCAGGTCT CTCTGGGTCA AAATTGACCT GACCCTGCTG 
TCCAGGATCC CAGAACACCT GCCCCAGGAG CCCGGGGTCT TGAATGCCCC TGCGGCCAAG 
GACGCCGAGA GCACGCAGCC AAGCCATCCG TCTGACGCTC CTGCCGAAAA GGCTTTGCCA 
AAATCCAAGA GGAAACGCAA GTGTGACAAT GAAGATGATT ACAGGGAGGT CAAGAAGGCC 
CAGGGAGAGA AAGAGAGCTC TTCGCGACTG ACCACCTCTG CCAATAATAC TTTATCTGCA 
AACCATTGCA GCATGAACAT CAACAGCTTG GCAATACCAA TAAATAAAAA TGAAAAAGTG 
CTCCGGTCAC CCATCTCACC CCTCTCTGAT GCATCTAAAC ACAAATACTC CAGCGAGGAC 
TTAACTTCTA GCAGATCAAA TGGCAACTTC ACTTCTGCTT CCTCCAACAA AAAGCATAAG 
GCTGAGAGCC AGCTGCAGTC TCATGCTGGA GACCTCACGA AAGCTGCTCA CAACAATTCC 
GAAAACGTTC TCCACAAGCC ACGGCCACAA ACAGAGCCAT GGTCTCCAGG TTCCAACGGC 
CACAGGGATT GCAAGAGGCA GAAACTTGTC TTCGATGATA TGCCACGCAG TGCAGATTAT 
TTTATGCAAG AAGCTAAACG GATGAAGCAT AAAGCAGATG CAATGGTGGA AAAGTTTGGA 
AAGGCTTTGA ACTATGCTGA AGCAGCTTTG TCATTTATTG AGTGTGGAAA TGCAATGGAA 
CAAGGCCCAA TGGAATCCAA ATCTCCTTAT ACAATGTATT CGGAAACAGT AGAGCTCATC 
AGGTATGCTA TGAGACTAAA AACTCATTCA GGCCCCAATG CCACACCAGA GGACAAACAA 
CTGGCTGCAT TATGTTACCG CTGCCTGGCT CTCCTGTACT GGCGGATGTT TCGACTCAAA 
AGGGACCATG CTGTGAAGTA TTCAAAAGCA CTTATTGACT ATTTCAAGAA TTCATCTAAA 
GCTGCCCAAG CCCCATCTCC ATGGGGAGCC AGTGGAAAGA GCACTGGAAC CCCATCCCCT 
ATGTCTCCCA ACCCCTCCCC AACCAGCTCC GTGGGATCTC AGGGGAGCCT CTCCAACGCC 
AGCGCCCTGT CCCCTTCAAC CATCGTCAGC ATCCCACAGC GCATCCACCA GATGGCAGCC 
AATCACGTCA GCATCACCAA CAGCATTCTC CACAGCTATG ACTACTGGGA GATGGCAGAC 
AACCTGGCCA AGGAAAACAG AGAATTCTTC AATGACCTGG ATTTGCTCAT GGGGCCTGTC 
ACCCTGCACA GCAGCATGGA GCACCTGGTC CAGTACTCCC AGCAGGGTCT GCACTGGCTG 
CGGAACAGCA CCCACCTGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972943.1
CDS	211..4014
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_014117468.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117468.2

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGC AGGCAGCCCA GAGAACTGCT CTCCACGCTC TGTCTGACAG CACCGTGGTC 
CAGCAGGCCA ACTGCAGAGC CTCTGTGCCT TCCAGCAAGG GCAGCAGCAG TGGCAGTAGC 
AGCAGCGGCA GCAGCTCCTC CAGTGACTCA GAGAGCAGCT CTGGATCTGA CTCGGAGACC 
GAGAGCAGTT CCAGCGAGAG TGAGGGCAGC AAGCCTCCCC ACTACTCCAG CCCCGAGGCT 
GAACCAGCGT CCTCTAACAA GTGGCAGCTG GACAAATGGC TAAACAAAGT TAATCCCCAC 
AAGCCGCCTA TTCTGATCCA AAATGAGAGC CACGGGCCAG AGAGCAATCA ATACTACGCT 
CCTGCGAAAG AGGAAGTGCA GGACTGTGCG AAGCTGGCCG ACGTTTGCCA AGCCAGCCTG 
CGAGACAAGG ACATCAAGAG CGCCTGCAAG GAGGAGCAGA GGCCAAGGAC TGCGAACAAG 
GCCCCGGGGA GCAAGGGAGT GAAGCAGAAG TCGCCGCCCG CCGCCGTGGC CGTGGCCGTG 
AGCGCAGCCG CCCAGCCGCC CGCCGCGCCC GGTGCGCCCG CCGCCGAGAG CACACCTGCG 
CCTGCCCGGA GGGCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGACAGGAC CGCCGCCGGC 
GACGCCACCA ACTGCCACCG GCCCGAGGAG CCCGCGGCTG CCGACGCGCT GGGGGCGAGC 
GTGGTGGGCC CCCCGGAGCC CGCCAAGACC AGGCCCTGCG GCAACAGCAG AACGAGCCAC 
CGCAAGGAGC TGCGCTCCTC GGTGACCTGC GAGAAGCGCC GCACGCGGGG GCTGAGCAGG 
ATCGTCCCCA AATCCAAGGA GTTCATAGAC ACAGAATCCT CCTCTTCCTC CTCCTCCTCG 
GACTCGGACC TGGAGTCCGA GCAAGAGGAG TACCCTCTGT CCAAAGCGCA GGCCGTGGCC 
GCCGCCGCCT CCTCGGCGAG TGACCAGAGG CTGAAGGAGG CTGCGGGCCC CATCAGCAGT 
GGCGGCGGCC CCCGGGCCCC CGTAGGCTCC ATCAACGCCA GGACCACCAG CGACATTGCC 
AAGGAGCTGG AGGAGCAGTT CTACACGCTC GTCCCTTTTG GCCGCAACGA ACTTCTCTCC 
CCCTTGAAGG ACAGCGATGA GGTCAGGTCT CTCTGGGTCA AAATTGACCT GACCCTGCTG 
TCCAGGATCC CAGAACACCT GCCCCAGGAG CCCGGGGTCT TGAATGCCCC TGCGGCCAAG 
GACGCCGAGA GCACGCAGCC AAGCCATCCG TCTGACGCTC CTGCCGAAAA GGCTTTGCCA 
AAATCCAAGA GGAAACGCAA GTGTGACAAT GAAGATGATT ACAGGGAGGT CAAGAAGGCC 
CAGGGAGAGA AAGAGAGCTC TTCGCGACTG ACCACCTCTG CCAATAATAC TTTATCTGCA 
AACCATTGCA GCATGAACAT CAACAGCTTG GCAATACCAA TAAATAAAAA TGAAAAAGTG 
CTCCGGTCAC CCATCTCACC CCTCTCTGAT GCATCTAAAC ACAAATACTC CAGCGAGGAC 
TTAACTTCTA GCAGATCAAA TGGCAACTTC ACTTCTGCTT CCTCCAACAA AAAGCATAAG 
GCTGAGAGCC AGCTGCAGTC TCATGCTGGA GACCTCACGA AAGCTGCTCA CAACAATTCC 
GAAAACGTTC TCCACAAGCC ACGGCCACAA ACAGAGCCAT GGTCTCCAGG TTCCAACGGC 
CACAGGGATT GCAAGAGGCA GAAACTTGTC TTCGATGATA TGCCACGCAG TGCAGATTAT 
TTTATGCAAG AAGCTAAACG GATGAAGCAT AAAGCAGATG CAATGGTGGA AAAGTTTGGA 
AAGGCTTTGA ACTATGCTGA AGCAGCTTTG TCATTTATTG AGTGTGGAAA TGCAATGGAA 
CAAGGCCCAA TGGAATCCAA ATCTCCTTAT ACAATGTATT CGGAAACAGT AGAGCTCATC 
AGGTATGCTA TGAGACTAAA AACTCATTCA GGCCCCAATG CCACACCAGA GGACAAACAA 
CTGGCTGCAT TATGTTACCG CTGCCTGGCT CTCCTGTACT GGCGGATGTT TCGACTCAAA 
AGGGACCATG CTGTGAAGTA TTCAAAAGCA CTTATTGACT ATTTCAAGAA TTCATCTAAA 
GCTGCCCAAG CCCCATCTCC ATGGGGAGCC AGTGGAAAGA GCACTGGAAC CCCATCCCCT 
ATGTCTCCCA ACCCCTCCCC AACCAGCTCC GTGGGATCTC AGGGGAGCCT CTCCAACGCC 
AGCGCCCTGT CCCCTTCAAC CATCGTCAGC ATCCCACAGC GCATCCACCA GATGGCAGCC 
AATCACGTCA GCATCACCAA CAGCATTCTC CACAGCTATG ACTACTGGGA GATGGCAGAC 
AACCTGGCCA AGGAAAACAG AGAATTCTTC AATGACCTGG ATTTGCTCAT GGGGCCTGTC 
ACCCTGCACA GCAGCATGGA GCACCTGGTC CAGTACTCCC AGCAGGGTCT GCACTGGCTG 
CGGAACAGCA CCCACCTGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_014117468.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972943.1 Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X1, mRNA.

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CloneID	OCe38981
Clone ID Related Accession (Same CDS sequence)	XM_014117468.2 , XM_014117468.1
Accession Version	XM_014117468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGC AGGCAGCCCA GAGAACTGCT CTCCACGCTC TGTCTGACAG CACCGTGGTC 
CAGCAGGCCA ACTGCAGAGC CTCTGTGCCT TCCAGCAAGG GCAGCAGCAG TGGCAGTAGC 
AGCAGCGGCA GCAGCTCCTC CAGTGACTCA GAGAGCAGCT CTGGATCTGA CTCGGAGACC 
GAGAGCAGTT CCAGCGAGAG TGAGGGCAGC AAGCCTCCCC ACTACTCCAG CCCCGAGGCT 
GAACCAGCGT CCTCTAACAA GTGGCAGCTG GACAAATGGC TAAACAAAGT TAATCCCCAC 
AAGCCGCCTA TTCTGATCCA AAATGAGAGC CACGGGCCAG AGAGCAATCA ATACTACGCT 
CCTGCGAAAG AGGAAGTGCA GGACTGTGCG AAGCTGGCCG ACGTTTGCCA AGCCAGCCTG 
CGAGACAAGG ACATCAAGAG CGCCTGCAAG GAGGAGCAGA GGCCAAGGAC TGCGAACAAG 
GCCCCGGGGA GCAAGGGAGT GAAGCAGAAG TCGCCGCCCG CCGCCGTGGC CGTGGCCGTG 
AGCGCAGCCG CCCAGCCGCC CGCCGCGCCC GGTGCGCCCG CCGCCGAGAG CACACCTGCG 
CCTGCCCGGA GGGCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGACAGGAC CGCCGCCGGC 
GACGCCACCA ACTGCCACCG GCCCGAGGAG CCCGCGGCTG CCGACGCGCT GGGGGCGAGC 
GTGGTGGGCC CCCCGGAGCC CGCCAAGACC AGGCCCTGCG GCAACAGCAG AACGAGCCAC 
CGCAAGGAGC TGCGCTCCTC GGTGACCTGC GAGAAGCGCC GCACGCGGGG GCTGAGCAGG 
ATCGTCCCCA AATCCAAGGA GTTCATAGAC ACAGAATCCT CCTCTTCCTC CTCCTCCTCG 
GACTCGGACC TGGAGTCCGA GCAAGAGGAG TACCCTCTGT CCAAAGCGCA GGCCGTGGCC 
GCCGCCGCCT CCTCGGCGAG TGACCAGAGG CTGAAGGAGG CTGCGGGCCC CATCAGCAGT 
GGCGGCGGCC CCCGGGCCCC CGTAGGCTCC ATCAACGCCA GGACCACCAG CGACATTGCC 
AAGGAGCTGG AGGAGCAGTT CTACACGCTC GTCCCTTTTG GCCGCAACGA ACTTCTCTCC 
CCCTTGAAGG ACAGCGATGA GGTCAGGTCT CTCTGGGTCA AAATTGACCT GACCCTGCTG 
TCCAGGATCC CAGAACACCT GCCCCAGGAG CCCGGGGTCT TGAATGCCCC TGCGGCCAAG 
GACGCCGAGA GCACGCAGCC AAGCCATCCG TCTGACGCTC CTGCCGAAAA GGCTTTGCCA 
AAATCCAAGA GGAAACGCAA GTGTGACAAT GAAGATGATT ACAGGGAGGT CAAGAAGGCC 
CAGGGAGAGA AAGAGAGCTC TTCGCGACTG ACCACCTCTG CCAATAATAC TTTATCTGCA 
AACCATTGCA GCATGAACAT CAACAGCTTG GCAATACCAA TAAATAAAAA TGAAAAAGTG 
CTCCGGTCAC CCATCTCACC CCTCTCTGAT GCATCTAAAC ACAAATACTC CAGCGAGGAC 
TTAACTTCTA GCAGATCAAA TGGCAACTTC ACTTCTGCTT CCTCCAACAA AAAGCATAAG 
GCTGAGAGCC AGCTGCAGTC TCATGCTGGA GACCTCACGA AAGCTGCTCA CAACAATTCC 
GAAAACGTTC TCCACAAGCC ACGGCCACAA ACAGAGCCAT GGTCTCCAGG TTCCAACGGC 
CACAGGGATT GCAAGAGGCA GAAACTTGTC TTCGATGATA TGCCACGCAG TGCAGATTAT 
TTTATGCAAG AAGCTAAACG GATGAAGCAT AAAGCAGATG CAATGGTGGA AAAGTTTGGA 
AAGGCTTTGA ACTATGCTGA AGCAGCTTTG TCATTTATTG AGTGTGGAAA TGCAATGGAA 
CAAGGCCCAA TGGAATCCAA ATCTCCTTAT ACAATGTATT CGGAAACAGT AGAGCTCATC 
AGGTATGCTA TGAGACTAAA AACTCATTCA GGCCCCAATG CCACACCAGA GGACAAACAA 
CTGGCTGCAT TATGTTACCG CTGCCTGGCT CTCCTGTACT GGCGGATGTT TCGACTCAAA 
AGGGACCATG CTGTGAAGTA TTCAAAAGCA CTTATTGACT ATTTCAAGAA TTCATCTAAA 
GCTGCCCAAG CCCCATCTCC ATGGGGAGCC AGTGGAAAGA GCACTGGAAC CCCATCCCCT 
ATGTCTCCCA ACCCCTCCCC AACCAGCTCC GTGGGATCTC AGGGGAGCCT CTCCAACGCC 
AGCGCCCTGT CCCCTTCAAC CATCGTCAGC ATCCCACAGC GCATCCACCA GATGGCAGCC 
AATCACGTCA GCATCACCAA CAGCATTCTC CACAGCTATG ACTACTGGGA GATGGCAGAC 
AACCTGGCCA AGGAAAACAG AGAATTCTTC AATGACCTGG ATTTGCTCAT GGGGCCTGTC 
ACCCTGCACA GCAGCATGGA GCACCTGGTC CAGTACTCCC AGCAGGGTCT GCACTGGCTG 
CGGAACAGCA CCCACCTGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972943.1
CDS	217..4020
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_014117468.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117468.1

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGC AGGCAGCCCA GAGAACTGCT CTCCACGCTC TGTCTGACAG CACCGTGGTC 
CAGCAGGCCA ACTGCAGAGC CTCTGTGCCT TCCAGCAAGG GCAGCAGCAG TGGCAGTAGC 
AGCAGCGGCA GCAGCTCCTC CAGTGACTCA GAGAGCAGCT CTGGATCTGA CTCGGAGACC 
GAGAGCAGTT CCAGCGAGAG TGAGGGCAGC AAGCCTCCCC ACTACTCCAG CCCCGAGGCT 
GAACCAGCGT CCTCTAACAA GTGGCAGCTG GACAAATGGC TAAACAAAGT TAATCCCCAC 
AAGCCGCCTA TTCTGATCCA AAATGAGAGC CACGGGCCAG AGAGCAATCA ATACTACGCT 
CCTGCGAAAG AGGAAGTGCA GGACTGTGCG AAGCTGGCCG ACGTTTGCCA AGCCAGCCTG 
CGAGACAAGG ACATCAAGAG CGCCTGCAAG GAGGAGCAGA GGCCAAGGAC TGCGAACAAG 
GCCCCGGGGA GCAAGGGAGT GAAGCAGAAG TCGCCGCCCG CCGCCGTGGC CGTGGCCGTG 
AGCGCAGCCG CCCAGCCGCC CGCCGCGCCC GGTGCGCCCG CCGCCGAGAG CACACCTGCG 
CCTGCCCGGA GGGCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGACAGGAC CGCCGCCGGC 
GACGCCACCA ACTGCCACCG GCCCGAGGAG CCCGCGGCTG CCGACGCGCT GGGGGCGAGC 
GTGGTGGGCC CCCCGGAGCC CGCCAAGACC AGGCCCTGCG GCAACAGCAG AACGAGCCAC 
CGCAAGGAGC TGCGCTCCTC GGTGACCTGC GAGAAGCGCC GCACGCGGGG GCTGAGCAGG 
ATCGTCCCCA AATCCAAGGA GTTCATAGAC ACAGAATCCT CCTCTTCCTC CTCCTCCTCG 
GACTCGGACC TGGAGTCCGA GCAAGAGGAG TACCCTCTGT CCAAAGCGCA GGCCGTGGCC 
GCCGCCGCCT CCTCGGCGAG TGACCAGAGG CTGAAGGAGG CTGCGGGCCC CATCAGCAGT 
GGCGGCGGCC CCCGGGCCCC CGTAGGCTCC ATCAACGCCA GGACCACCAG CGACATTGCC 
AAGGAGCTGG AGGAGCAGTT CTACACGCTC GTCCCTTTTG GCCGCAACGA ACTTCTCTCC 
CCCTTGAAGG ACAGCGATGA GGTCAGGTCT CTCTGGGTCA AAATTGACCT GACCCTGCTG 
TCCAGGATCC CAGAACACCT GCCCCAGGAG CCCGGGGTCT TGAATGCCCC TGCGGCCAAG 
GACGCCGAGA GCACGCAGCC AAGCCATCCG TCTGACGCTC CTGCCGAAAA GGCTTTGCCA 
AAATCCAAGA GGAAACGCAA GTGTGACAAT GAAGATGATT ACAGGGAGGT CAAGAAGGCC 
CAGGGAGAGA AAGAGAGCTC TTCGCGACTG ACCACCTCTG CCAATAATAC TTTATCTGCA 
AACCATTGCA GCATGAACAT CAACAGCTTG GCAATACCAA TAAATAAAAA TGAAAAAGTG 
CTCCGGTCAC CCATCTCACC CCTCTCTGAT GCATCTAAAC ACAAATACTC CAGCGAGGAC 
TTAACTTCTA GCAGATCAAA TGGCAACTTC ACTTCTGCTT CCTCCAACAA AAAGCATAAG 
GCTGAGAGCC AGCTGCAGTC TCATGCTGGA GACCTCACGA AAGCTGCTCA CAACAATTCC 
GAAAACGTTC TCCACAAGCC ACGGCCACAA ACAGAGCCAT GGTCTCCAGG TTCCAACGGC 
CACAGGGATT GCAAGAGGCA GAAACTTGTC TTCGATGATA TGCCACGCAG TGCAGATTAT 
TTTATGCAAG AAGCTAAACG GATGAAGCAT AAAGCAGATG CAATGGTGGA AAAGTTTGGA 
AAGGCTTTGA ACTATGCTGA AGCAGCTTTG TCATTTATTG AGTGTGGAAA TGCAATGGAA 
CAAGGCCCAA TGGAATCCAA ATCTCCTTAT ACAATGTATT CGGAAACAGT AGAGCTCATC 
AGGTATGCTA TGAGACTAAA AACTCATTCA GGCCCCAATG CCACACCAGA GGACAAACAA 
CTGGCTGCAT TATGTTACCG CTGCCTGGCT CTCCTGTACT GGCGGATGTT TCGACTCAAA 
AGGGACCATG CTGTGAAGTA TTCAAAAGCA CTTATTGACT ATTTCAAGAA TTCATCTAAA 
GCTGCCCAAG CCCCATCTCC ATGGGGAGCC AGTGGAAAGA GCACTGGAAC CCCATCCCCT 
ATGTCTCCCA ACCCCTCCCC AACCAGCTCC GTGGGATCTC AGGGGAGCCT CTCCAACGCC 
AGCGCCCTGT CCCCTTCAAC CATCGTCAGC ATCCCACAGC GCATCCACCA GATGGCAGCC 
AATCACGTCA GCATCACCAA CAGCATTCTC CACAGCTATG ACTACTGGGA GATGGCAGAC 
AACCTGGCCA AGGAAAACAG AGAATTCTTC AATGACCTGG ATTTGCTCAT GGGGCCTGTC 
ACCCTGCACA GCAGCATGGA GCACCTGGTC CAGTACTCCC AGCAGGGTCT GCACTGGCTG 
CGGAACAGCA CCCACCTGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_014117469.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972944.1 Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X2, mRNA.

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CloneID	OCe38982
Clone ID Related Accession (Same CDS sequence)	XM_014117469.1 , XM_014117469.2
Accession Version	XM_014117469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	AF4/FMR2 family member 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACTGCTCTC CACGCTCTGT CTGACAGCAC CGTGGTCCAG 
CAGGCCAACT GCAGAGCCTC TGTGCCTTCC AGCAAGGGCA GCAGCAGTGG CAGTAGCAGC 
AGCGGCAGCA GCTCCTCCAG TGACTCAGAG AGCAGCTCTG GATCTGACTC GGAGACCGAG 
AGCAGTTCCA GCGAGAGTGA GGGCAGCAAG CCTCCCCACT ACTCCAGCCC CGAGGCTGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAC AAATGGCTAA ACAAAGTTAA TCCCCACAAG 
CCGCCTATTC TGATCCAAAA TGAGAGCCAC GGGCCAGAGA GCAATCAATA CTACGCTCCT 
GCGAAAGAGG AAGTGCAGGA CTGTGCGAAG CTGGCCGACG TTTGCCAAGC CAGCCTGCGA 
GACAAGGACA TCAAGAGCGC CTGCAAGGAG GAGCAGAGGC CAAGGACTGC GAACAAGGCC 
CCGGGGAGCA AGGGAGTGAA GCAGAAGTCG CCGCCCGCCG CCGTGGCCGT GGCCGTGAGC 
GCAGCCGCCC AGCCGCCCGC CGCGCCCGGT GCGCCCGCCG CCGAGAGCAC ACCTGCGCCT 
GCCCGGAGGG CCGCGGGCAA GAAGCCCACC CGGCGCACCG ACAGGACCGC CGCCGGCGAC 
GCCACCAACT GCCACCGGCC CGAGGAGCCC GCGGCTGCCG ACGCGCTGGG GGCGAGCGTG 
GTGGGCCCCC CGGAGCCCGC CAAGACCAGG CCCTGCGGCA ACAGCAGAAC GAGCCACCGC 
AAGGAGCTGC GCTCCTCGGT GACCTGCGAG AAGCGCCGCA CGCGGGGGCT GAGCAGGATC 
GTCCCCAAAT CCAAGGAGTT CATAGACACA GAATCCTCCT CTTCCTCCTC CTCCTCGGAC 
TCGGACCTGG AGTCCGAGCA AGAGGAGTAC CCTCTGTCCA AAGCGCAGGC CGTGGCCGCC 
GCCGCCTCCT CGGCGAGTGA CCAGAGGCTG AAGGAGGCTG CGGGCCCCAT CAGCAGTGGC 
GGCGGCCCCC GGGCCCCCGT AGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACGCTCGTC CCTTTTGGCC GCAACGAACT TCTCTCCCCC 
TTGAAGGACA GCGATGAGGT CAGGTCTCTC TGGGTCAAAA TTGACCTGAC CCTGCTGTCC 
AGGATCCCAG AACACCTGCC CCAGGAGCCC GGGGTCTTGA ATGCCCCTGC GGCCAAGGAC 
GCCGAGAGCA CGCAGCCAAG CCATCCGTCT GACGCTCCTG CCGAAAAGGC TTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAATGAA GATGATTACA GGGAGGTCAA GAAGGCCCAG 
GGAGAGAAAG AGAGCTCTTC GCGACTGACC ACCTCTGCCA ATAATACTTT ATCTGCAAAC 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCACCCA TCTCACCCCT CTCTGATGCA TCTAAACACA AATACTCCAG CGAGGACTTA 
ACTTCTAGCA GATCAAATGG CAACTTCACT TCTGCTTCCT CCAACAAAAA GCATAAGGCT 
GAGAGCCAGC TGCAGTCTCA TGCTGGAGAC CTCACGAAAG CTGCTCACAA CAATTCCGAA 
AACGTTCTCC ACAAGCCACG GCCACAAACA GAGCCATGGT CTCCAGGTTC CAACGGCCAC 
AGGGATTGCA AGAGGCAGAA ACTTGTCTTC GATGATATGC CACGCAGTGC AGATTATTTT 
ATGCAAGAAG CTAAACGGAT GAAGCATAAA GCAGATGCAA TGGTGGAAAA GTTTGGAAAG 
GCTTTGAACT ATGCTGAAGC AGCTTTGTCA TTTATTGAGT GTGGAAATGC AATGGAACAA 
GGCCCAATGG AATCCAAATC TCCTTATACA ATGTATTCGG AAACAGTAGA GCTCATCAGG 
TATGCTATGA GACTAAAAAC TCATTCAGGC CCCAATGCCA CACCAGAGGA CAAACAACTG 
GCTGCATTAT GTTACCGCTG CCTGGCTCTC CTGTACTGGC GGATGTTTCG ACTCAAAAGG 
GACCATGCTG TGAAGTATTC AAAAGCACTT ATTGACTATT TCAAGAATTC ATCTAAAGCT 
GCCCAAGCCC CATCTCCATG GGGAGCCAGT GGAAAGAGCA CTGGAACCCC ATCCCCTATG 
TCTCCCAACC CCTCCCCAAC CAGCTCCGTG GGATCTCAGG GGAGCCTCTC CAACGCCAGC 
GCCCTGTCCC CTTCAACCAT CGTCAGCATC CCACAGCGCA TCCACCAGAT GGCAGCCAAT 
CACGTCAGCA TCACCAACAG CATTCTCCAC AGCTATGACT ACTGGGAGAT GGCAGACAAC 
CTGGCCAAGG AAAACAGAGA ATTCTTCAAT GACCTGGATT TGCTCATGGG GCCTGTCACC 
CTGCACAGCA GCATGGAGCA CCTGGTCCAG TACTCCCAGC AGGGTCTGCA CTGGCTGCGG 
AACAGCACCC ACCTGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972944.1
CDS	217..4017
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_014117469.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris AF4/FMR2 family, member 3 (AFF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117469.1

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACTGCTCTC CACGCTCTGT CTGACAGCAC CGTGGTCCAG 
CAGGCCAACT GCAGAGCCTC TGTGCCTTCC AGCAAGGGCA GCAGCAGTGG CAGTAGCAGC 
AGCGGCAGCA GCTCCTCCAG TGACTCAGAG AGCAGCTCTG GATCTGACTC GGAGACCGAG 
AGCAGTTCCA GCGAGAGTGA GGGCAGCAAG CCTCCCCACT ACTCCAGCCC CGAGGCTGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAC AAATGGCTAA ACAAAGTTAA TCCCCACAAG 
CCGCCTATTC TGATCCAAAA TGAGAGCCAC GGGCCAGAGA GCAATCAATA CTACGCTCCT 
GCGAAAGAGG AAGTGCAGGA CTGTGCGAAG CTGGCCGACG TTTGCCAAGC CAGCCTGCGA 
GACAAGGACA TCAAGAGCGC CTGCAAGGAG GAGCAGAGGC CAAGGACTGC GAACAAGGCC 
CCGGGGAGCA AGGGAGTGAA GCAGAAGTCG CCGCCCGCCG CCGTGGCCGT GGCCGTGAGC 
GCAGCCGCCC AGCCGCCCGC CGCGCCCGGT GCGCCCGCCG CCGAGAGCAC ACCTGCGCCT 
GCCCGGAGGG CCGCGGGCAA GAAGCCCACC CGGCGCACCG ACAGGACCGC CGCCGGCGAC 
GCCACCAACT GCCACCGGCC CGAGGAGCCC GCGGCTGCCG ACGCGCTGGG GGCGAGCGTG 
GTGGGCCCCC CGGAGCCCGC CAAGACCAGG CCCTGCGGCA ACAGCAGAAC GAGCCACCGC 
AAGGAGCTGC GCTCCTCGGT GACCTGCGAG AAGCGCCGCA CGCGGGGGCT GAGCAGGATC 
GTCCCCAAAT CCAAGGAGTT CATAGACACA GAATCCTCCT CTTCCTCCTC CTCCTCGGAC 
TCGGACCTGG AGTCCGAGCA AGAGGAGTAC CCTCTGTCCA AAGCGCAGGC CGTGGCCGCC 
GCCGCCTCCT CGGCGAGTGA CCAGAGGCTG AAGGAGGCTG CGGGCCCCAT CAGCAGTGGC 
GGCGGCCCCC GGGCCCCCGT AGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACGCTCGTC CCTTTTGGCC GCAACGAACT TCTCTCCCCC 
TTGAAGGACA GCGATGAGGT CAGGTCTCTC TGGGTCAAAA TTGACCTGAC CCTGCTGTCC 
AGGATCCCAG AACACCTGCC CCAGGAGCCC GGGGTCTTGA ATGCCCCTGC GGCCAAGGAC 
GCCGAGAGCA CGCAGCCAAG CCATCCGTCT GACGCTCCTG CCGAAAAGGC TTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAATGAA GATGATTACA GGGAGGTCAA GAAGGCCCAG 
GGAGAGAAAG AGAGCTCTTC GCGACTGACC ACCTCTGCCA ATAATACTTT ATCTGCAAAC 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCACCCA TCTCACCCCT CTCTGATGCA TCTAAACACA AATACTCCAG CGAGGACTTA 
ACTTCTAGCA GATCAAATGG CAACTTCACT TCTGCTTCCT CCAACAAAAA GCATAAGGCT 
GAGAGCCAGC TGCAGTCTCA TGCTGGAGAC CTCACGAAAG CTGCTCACAA CAATTCCGAA 
AACGTTCTCC ACAAGCCACG GCCACAAACA GAGCCATGGT CTCCAGGTTC CAACGGCCAC 
AGGGATTGCA AGAGGCAGAA ACTTGTCTTC GATGATATGC CACGCAGTGC AGATTATTTT 
ATGCAAGAAG CTAAACGGAT GAAGCATAAA GCAGATGCAA TGGTGGAAAA GTTTGGAAAG 
GCTTTGAACT ATGCTGAAGC AGCTTTGTCA TTTATTGAGT GTGGAAATGC AATGGAACAA 
GGCCCAATGG AATCCAAATC TCCTTATACA ATGTATTCGG AAACAGTAGA GCTCATCAGG 
TATGCTATGA GACTAAAAAC TCATTCAGGC CCCAATGCCA CACCAGAGGA CAAACAACTG 
GCTGCATTAT GTTACCGCTG CCTGGCTCTC CTGTACTGGC GGATGTTTCG ACTCAAAAGG 
GACCATGCTG TGAAGTATTC AAAAGCACTT ATTGACTATT TCAAGAATTC ATCTAAAGCT 
GCCCAAGCCC CATCTCCATG GGGAGCCAGT GGAAAGAGCA CTGGAACCCC ATCCCCTATG 
TCTCCCAACC CCTCCCCAAC CAGCTCCGTG GGATCTCAGG GGAGCCTCTC CAACGCCAGC 
GCCCTGTCCC CTTCAACCAT CGTCAGCATC CCACAGCGCA TCCACCAGAT GGCAGCCAAT 
CACGTCAGCA TCACCAACAG CATTCTCCAC AGCTATGACT ACTGGGAGAT GGCAGACAAC 
CTGGCCAAGG AAAACAGAGA ATTCTTCAAT GACCTGGATT TGCTCATGGG GCCTGTCACC 
CTGCACAGCA GCATGGAGCA CCTGGTCCAG TACTCCCAGC AGGGTCTGCA CTGGCTGCGG 
AACAGCACCC ACCTGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_014117469.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972944.1 Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

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CloneID	OCe38982
Clone ID Related Accession (Same CDS sequence)	XM_014117469.1 , XM_014117469.2
Accession Version	XM_014117469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	AF4/FMR2 family member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACTGCTCTC CACGCTCTGT CTGACAGCAC CGTGGTCCAG 
CAGGCCAACT GCAGAGCCTC TGTGCCTTCC AGCAAGGGCA GCAGCAGTGG CAGTAGCAGC 
AGCGGCAGCA GCTCCTCCAG TGACTCAGAG AGCAGCTCTG GATCTGACTC GGAGACCGAG 
AGCAGTTCCA GCGAGAGTGA GGGCAGCAAG CCTCCCCACT ACTCCAGCCC CGAGGCTGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAC AAATGGCTAA ACAAAGTTAA TCCCCACAAG 
CCGCCTATTC TGATCCAAAA TGAGAGCCAC GGGCCAGAGA GCAATCAATA CTACGCTCCT 
GCGAAAGAGG AAGTGCAGGA CTGTGCGAAG CTGGCCGACG TTTGCCAAGC CAGCCTGCGA 
GACAAGGACA TCAAGAGCGC CTGCAAGGAG GAGCAGAGGC CAAGGACTGC GAACAAGGCC 
CCGGGGAGCA AGGGAGTGAA GCAGAAGTCG CCGCCCGCCG CCGTGGCCGT GGCCGTGAGC 
GCAGCCGCCC AGCCGCCCGC CGCGCCCGGT GCGCCCGCCG CCGAGAGCAC ACCTGCGCCT 
GCCCGGAGGG CCGCGGGCAA GAAGCCCACC CGGCGCACCG ACAGGACCGC CGCCGGCGAC 
GCCACCAACT GCCACCGGCC CGAGGAGCCC GCGGCTGCCG ACGCGCTGGG GGCGAGCGTG 
GTGGGCCCCC CGGAGCCCGC CAAGACCAGG CCCTGCGGCA ACAGCAGAAC GAGCCACCGC 
AAGGAGCTGC GCTCCTCGGT GACCTGCGAG AAGCGCCGCA CGCGGGGGCT GAGCAGGATC 
GTCCCCAAAT CCAAGGAGTT CATAGACACA GAATCCTCCT CTTCCTCCTC CTCCTCGGAC 
TCGGACCTGG AGTCCGAGCA AGAGGAGTAC CCTCTGTCCA AAGCGCAGGC CGTGGCCGCC 
GCCGCCTCCT CGGCGAGTGA CCAGAGGCTG AAGGAGGCTG CGGGCCCCAT CAGCAGTGGC 
GGCGGCCCCC GGGCCCCCGT AGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACGCTCGTC CCTTTTGGCC GCAACGAACT TCTCTCCCCC 
TTGAAGGACA GCGATGAGGT CAGGTCTCTC TGGGTCAAAA TTGACCTGAC CCTGCTGTCC 
AGGATCCCAG AACACCTGCC CCAGGAGCCC GGGGTCTTGA ATGCCCCTGC GGCCAAGGAC 
GCCGAGAGCA CGCAGCCAAG CCATCCGTCT GACGCTCCTG CCGAAAAGGC TTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAATGAA GATGATTACA GGGAGGTCAA GAAGGCCCAG 
GGAGAGAAAG AGAGCTCTTC GCGACTGACC ACCTCTGCCA ATAATACTTT ATCTGCAAAC 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCACCCA TCTCACCCCT CTCTGATGCA TCTAAACACA AATACTCCAG CGAGGACTTA 
ACTTCTAGCA GATCAAATGG CAACTTCACT TCTGCTTCCT CCAACAAAAA GCATAAGGCT 
GAGAGCCAGC TGCAGTCTCA TGCTGGAGAC CTCACGAAAG CTGCTCACAA CAATTCCGAA 
AACGTTCTCC ACAAGCCACG GCCACAAACA GAGCCATGGT CTCCAGGTTC CAACGGCCAC 
AGGGATTGCA AGAGGCAGAA ACTTGTCTTC GATGATATGC CACGCAGTGC AGATTATTTT 
ATGCAAGAAG CTAAACGGAT GAAGCATAAA GCAGATGCAA TGGTGGAAAA GTTTGGAAAG 
GCTTTGAACT ATGCTGAAGC AGCTTTGTCA TTTATTGAGT GTGGAAATGC AATGGAACAA 
GGCCCAATGG AATCCAAATC TCCTTATACA ATGTATTCGG AAACAGTAGA GCTCATCAGG 
TATGCTATGA GACTAAAAAC TCATTCAGGC CCCAATGCCA CACCAGAGGA CAAACAACTG 
GCTGCATTAT GTTACCGCTG CCTGGCTCTC CTGTACTGGC GGATGTTTCG ACTCAAAAGG 
GACCATGCTG TGAAGTATTC AAAAGCACTT ATTGACTATT TCAAGAATTC ATCTAAAGCT 
GCCCAAGCCC CATCTCCATG GGGAGCCAGT GGAAAGAGCA CTGGAACCCC ATCCCCTATG 
TCTCCCAACC CCTCCCCAAC CAGCTCCGTG GGATCTCAGG GGAGCCTCTC CAACGCCAGC 
GCCCTGTCCC CTTCAACCAT CGTCAGCATC CCACAGCGCA TCCACCAGAT GGCAGCCAAT 
CACGTCAGCA TCACCAACAG CATTCTCCAC AGCTATGACT ACTGGGAGAT GGCAGACAAC 
CTGGCCAAGG AAAACAGAGA ATTCTTCAAT GACCTGGATT TGCTCATGGG GCCTGTCACC 
CTGCACAGCA GCATGGAGCA CCTGGTCCAG TACTCCCAGC AGGGTCTGCA CTGGCTGCGG 
AACAGCACCC ACCTGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972944.1
CDS	211..4011
Translation	MNICWNEHINCTLFCLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVNKIDEHFAADSRAQAQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSTETGVHCTSYRGVPATKPESARAKAKLSKLSIPKQGEESRSGDTNSCVEEIIREMTWLPPLSAIPAPGKVEPSKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSSLEWLSPFTDKGNDAQRGMLEDDLKLSSDEEENEQAAQRTALHALSDSTVVQQANCRASVPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPESNQYYAPAKEEVQDCAKLADVCQASLRDKDIKSACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAQPPAAPGAPAAESTPAPARRAAGKKPTRRTDRTAAGDATNCHRPEEPAAADALGASVVGPPEPAKTRPCGNSRTSHRKELRSSVTCEKRRTRGLSRIVPKSKEFIDTESSSSSSSSDSDLESEQEEYPLSKAQAVAAAASSASDQRLKEAAGPISSGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLNAPAAKDAESTQPSHPSDAPAEKALPKSKRKRKCDNEDDYREVKKAQGEKESSSRLTTSANNTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKYSSEDLTSSRSNGNFTSASSNKKHKAESQLQSHAGDLTKAAHNNSENVLHKPRPQTEPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTSSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSTHLS

Target ORF information:

RefSeq Version	XM_014117469.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117469.2

ORF Insert Sequence:

ATGAACATCT GCTGGAACGA GCATATTAAC TGCACTCTGT TCTGCCTCTG GGGTGAAGAC 
ATCTTAAACC AGAGGAATGA CTCTCTAGTT GTGGAATTTC AGTCTTCCGC CAGCAGATGC 
AGGAGCGTCT ATGAACCAGA TAGAAATGTG TTACGAAGGA AGGAACGAGA GAGAAGAAAT 
CAGGAAACAC AGCAGGATGA TGGCACATTT AATTCGAGTT ACTCCCTCTT CAGTGAACCT 
TATAAGACTA ACAAGGGGGA TGAGCTCTCC AACCGGATCC AGAATACTTT AGGCAATTAC 
GATGAAATGA AAGACTTTTT AACTGATCGA TCTAATCAGA GTCATCTTGT TGGCGTTCCC 
AAACCTGGGG TTCCTCAGGC TCCCGTGAAC AAGATCGATG AACATTTTGC TGCAGATTCA 
AGAGCCCAGG CCCAGCCCTC ATCTGTCTGT AGCACTGCTT CTTCCACACC AGCAGCTGTC 
CCCGTGCAGC AGAGTAAGAG AGGCACCATG GGCTGGCAGA AGGCCGGGCA TCCGCCATCA 
GATGGCCAAC AGAGAGCAAC ACAACAAGGC TCTCTCAGGA CCTTGCTTGG AGATGGTGTT 
GGCAGACAGC AGCCACGAGC CAAACAAGTG TGCAATGTAG AGGTGGGTCT TCAGACCCAG 
GAAAGGCCAC CTGCTATGGC AGCCAAGCAT AGCAGCAGCG GACACTGTGT TCAGAACTTT 
CCTCCATCCC TGGCTTCAAA ACCCAGCCTG GTCCAGCAGA AACCAACTGC ATATGTGCGG 
CCGATGGATG GCCAAGATCA AGCTCCCGAC GAGTCTCCAA AGCTTAAGTC GTCCACAGAA 
ACGGGTGTGC ACTGCACCTC ATACAGGGGA GTCCCGGCCA CCAAGCCAGA GTCTGCCAGA 
GCCAAGGCCA AACTTTCCAA GTTAAGCATC CCCAAACAAG GAGAGGAGAG TAGATCTGGA 
GACACAAACA GCTGTGTTGA AGAAATAATT CGGGAGATGA CCTGGCTCCC ACCACTTTCT 
GCTATTCCAG CACCTGGAAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGTTT CCTCCGGACA CAATAATCCA AAGAAAGGTG ATGCTGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAATAC ATCCTCTCTG GAATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC AGAGAGGAAT GTTAGAAGAT GACCTTAAGC TAAGTAGTGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACTGCTCTC CACGCTCTGT CTGACAGCAC CGTGGTCCAG 
CAGGCCAACT GCAGAGCCTC TGTGCCTTCC AGCAAGGGCA GCAGCAGTGG CAGTAGCAGC 
AGCGGCAGCA GCTCCTCCAG TGACTCAGAG AGCAGCTCTG GATCTGACTC GGAGACCGAG 
AGCAGTTCCA GCGAGAGTGA GGGCAGCAAG CCTCCCCACT ACTCCAGCCC CGAGGCTGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAC AAATGGCTAA ACAAAGTTAA TCCCCACAAG 
CCGCCTATTC TGATCCAAAA TGAGAGCCAC GGGCCAGAGA GCAATCAATA CTACGCTCCT 
GCGAAAGAGG AAGTGCAGGA CTGTGCGAAG CTGGCCGACG TTTGCCAAGC CAGCCTGCGA 
GACAAGGACA TCAAGAGCGC CTGCAAGGAG GAGCAGAGGC CAAGGACTGC GAACAAGGCC 
CCGGGGAGCA AGGGAGTGAA GCAGAAGTCG CCGCCCGCCG CCGTGGCCGT GGCCGTGAGC 
GCAGCCGCCC AGCCGCCCGC CGCGCCCGGT GCGCCCGCCG CCGAGAGCAC ACCTGCGCCT 
GCCCGGAGGG CCGCGGGCAA GAAGCCCACC CGGCGCACCG ACAGGACCGC CGCCGGCGAC 
GCCACCAACT GCCACCGGCC CGAGGAGCCC GCGGCTGCCG ACGCGCTGGG GGCGAGCGTG 
GTGGGCCCCC CGGAGCCCGC CAAGACCAGG CCCTGCGGCA ACAGCAGAAC GAGCCACCGC 
AAGGAGCTGC GCTCCTCGGT GACCTGCGAG AAGCGCCGCA CGCGGGGGCT GAGCAGGATC 
GTCCCCAAAT CCAAGGAGTT CATAGACACA GAATCCTCCT CTTCCTCCTC CTCCTCGGAC 
TCGGACCTGG AGTCCGAGCA AGAGGAGTAC CCTCTGTCCA AAGCGCAGGC CGTGGCCGCC 
GCCGCCTCCT CGGCGAGTGA CCAGAGGCTG AAGGAGGCTG CGGGCCCCAT CAGCAGTGGC 
GGCGGCCCCC GGGCCCCCGT AGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACGCTCGTC CCTTTTGGCC GCAACGAACT TCTCTCCCCC 
TTGAAGGACA GCGATGAGGT CAGGTCTCTC TGGGTCAAAA TTGACCTGAC CCTGCTGTCC 
AGGATCCCAG AACACCTGCC CCAGGAGCCC GGGGTCTTGA ATGCCCCTGC GGCCAAGGAC 
GCCGAGAGCA CGCAGCCAAG CCATCCGTCT GACGCTCCTG CCGAAAAGGC TTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAATGAA GATGATTACA GGGAGGTCAA GAAGGCCCAG 
GGAGAGAAAG AGAGCTCTTC GCGACTGACC ACCTCTGCCA ATAATACTTT ATCTGCAAAC 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCACCCA TCTCACCCCT CTCTGATGCA TCTAAACACA AATACTCCAG CGAGGACTTA 
ACTTCTAGCA GATCAAATGG CAACTTCACT TCTGCTTCCT CCAACAAAAA GCATAAGGCT 
GAGAGCCAGC TGCAGTCTCA TGCTGGAGAC CTCACGAAAG CTGCTCACAA CAATTCCGAA 
AACGTTCTCC ACAAGCCACG GCCACAAACA GAGCCATGGT CTCCAGGTTC CAACGGCCAC 
AGGGATTGCA AGAGGCAGAA ACTTGTCTTC GATGATATGC CACGCAGTGC AGATTATTTT 
ATGCAAGAAG CTAAACGGAT GAAGCATAAA GCAGATGCAA TGGTGGAAAA GTTTGGAAAG 
GCTTTGAACT ATGCTGAAGC AGCTTTGTCA TTTATTGAGT GTGGAAATGC AATGGAACAA 
GGCCCAATGG AATCCAAATC TCCTTATACA ATGTATTCGG AAACAGTAGA GCTCATCAGG 
TATGCTATGA GACTAAAAAC TCATTCAGGC CCCAATGCCA CACCAGAGGA CAAACAACTG 
GCTGCATTAT GTTACCGCTG CCTGGCTCTC CTGTACTGGC GGATGTTTCG ACTCAAAAGG 
GACCATGCTG TGAAGTATTC AAAAGCACTT ATTGACTATT TCAAGAATTC ATCTAAAGCT 
GCCCAAGCCC CATCTCCATG GGGAGCCAGT GGAAAGAGCA CTGGAACCCC ATCCCCTATG 
TCTCCCAACC CCTCCCCAAC CAGCTCCGTG GGATCTCAGG GGAGCCTCTC CAACGCCAGC 
GCCCTGTCCC CTTCAACCAT CGTCAGCATC CCACAGCGCA TCCACCAGAT GGCAGCCAAT 
CACGTCAGCA TCACCAACAG CATTCTCCAC AGCTATGACT ACTGGGAGAT GGCAGACAAC 
CTGGCCAAGG AAAACAGAGA ATTCTTCAAT GACCTGGATT TGCTCATGGG GCCTGTCACC 
CTGCACAGCA GCATGGAGCA CCTGGTCCAG TACTCCCAGC AGGGTCTGCA CTGGCTGCGG 
AACAGCACCC ACCTGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFF3
Entrez Gene ID	611584
Full Name	AF4/FMR2 family member 3
General protein information	Preferred Names AF4/FMR2 family member 3 Names AF4/FMR2 family member 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	AFF3	NP_001020279.1
	LOC714408	XP_002808094.1
Canis lupus familiaris (dog)	AFF3	XP_005626071.1
Mus musculus (house mouse)	Aff3	NP_034808.2
Xenopus tropicalis (tropical clawed frog)	aff3	XP_002937914.2
Bos taurus (cattle)	AFF3	XP_002691193.1
Pan troglodytes (chimpanzee)	AFF3	XP_001160964.2
Rattus norvegicus (Norway rat)	Aff3	NP_001178816.1
Gallus gallus (chicken)	AFF3	XP_004938558.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFF3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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ORF Insert Sequence:

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