AFF3 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_002700703.5	XP_002700749.2	AF4/FMR2 family member 3 isoform X2
XM_015465297.1	XP_015320783.1	AF4/FMR2 family member 3 isoform X3
XM_002691147.5	XP_002691193.2	AF4/FMR2 family member 3 isoform X2
XM_024999313.1	XP_024855081.1	AF4/FMR2 family member 3 isoform X2
XM_024999314.1	XP_024855082.1	AF4/FMR2 family member 3 isoform X3
XM_024999316.1	XP_024855084.1	AF4/FMR2 family member 3 isoform X5
XM_024999317.1	XP_024855085.1	AF4/FMR2 family member 3 isoform X6
XM_024999318.1	XP_024855086.1	AF4/FMR2 family member 3 isoform X7
XM_002691147.6	XP_002691193.3	AF4/FMR2 family member 3 isoform X8
XM_024999319.1	XP_024855087.1	AF4/FMR2 family member 3 isoform X9
XM_010809809.2	XP_010808111.1	AF4/FMR2 family member 3 isoform X1
XM_010809810.2	XP_010808112.1	AF4/FMR2 family member 3 isoform X3
XM_015465296.1	XP_015320782.1	AF4/FMR2 family member 3 isoform X1
XM_024999312.1	XP_024855080.1	AF4/FMR2 family member 3 isoform X1
XM_024999315.1	XP_024855083.1	AF4/FMR2 family member 3 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220733	XM_024999312.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220734	XM_024999313.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220735	XM_024999314.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220736	XM_024999315.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220737	XM_024999316.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220738	XM_024999317.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220739	XM_024999318.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220740	XM_002691147.6 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220741	XM_024999319.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa58183	XM_002700703.5 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa58183	XM_002691147.5 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa58184	XM_015465296.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa58184	XM_010809809.2 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa58185	XM_010809810.2 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa58185	XM_015465297.1 Latest version!	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

AFF3 ( XM_024999312.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855080.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220733
Clone ID Related Accession (Same CDS sequence)	XM_024999312.1
Accession Version	XM_024999312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGC AGGCAGCCCA GAGAACGGCC 
CTCCATGCTC TGTCTGACAG TGCCGGACTC CAGCCGGCCG ACTGCAGGGC CTCCGCACCC 
TCGAGCAAGG GCAGCAGCAG CGGCAGCAGC AGCAGTGGGA GCAGCTCCTC CAGCGACTCG 
GAGAGCAGCT CGGGGTCCGA CTCGGAGACA GAGAGCAGTT CCAGCGAGAG CGAGGGCAGC 
AAGCCTCCCC ACTACTCCAG TCCCGAGGCG GAACCAGCGT CCTCTAACAA GTGGCAGCTG 
GATAAATGGC TAAACAAAGT GAATCACCAC AAGCCCCCTG TCCTGATCCA AAATGAAAGC 
CACCACGGGC CGGAGAGCAG TCAGTACTAC ACCCCAGTGA AGGAGGACAC GCAGGACTGC 
GGGAAGCTCC CCGACGTCTG CCCGCCCGGC CTGAGAGACA AGGACATCAA GGGCGCCTGC 
AAGGAGGAGC AGAGGCCCAG GACCGCGAAC AAGGCCCCCG GGAGCAAAGG GGTGAAGCAG 
AAGTCCCCGC CCGCGGCCGT GGCCGTGGCC GTGACCGCCG CCGCCGCCCC GCCGCCCGCG 
GTGCCTGGTG CGCCCGCCGA GAGCGCGCCC GCACCTGCCC GGAGGTCCGC GGGCAAGAAG 
CCCACCCGGC GCACCGAGAG GACCTCGGCC GGCGACAGCG GCCACCGGCC CGAGGAGCCC 
GTAGCTGCCG AGGCGCTGGG GGCGAGCACG GGGGTTCCCC CGGAGCCCCC CAAGCCCCGG 
CCTTGCGGCA ACAGCAGGAC CAACCACCGC AAGGAGCTGC GCTCGGCCGT GACCTGCGAG 
AAGCGCCGCA CTCGGGGGCT GAGCCGGATC GTCCCCAAGT CCAAGGAGTT CATCGAGACG 
GAGTCGTCCT CTTCCTCCTC CTCCTCGGAC TCGGACCTGG AGTCGGAGCA GGAGGACTAC 
CCTCTGTCCA AGGCCGTGGC CGCCGCCGCC GCCGCTGCCG CTGCCTCCGG GAACGACCCA 
AGGCTGAAGG AGGCTCCCAG CAGCATCAGC GGCGGCGGCC CCCGCGCTCC CGTGGGCTCC 
ATCAACGCCA GGACCACCAG CGACATTGCC AAGGAGCTGG AGGAGCAGTT CTACACACTG 
GTCCCCTTCG GCCGCAACGA GCTTCTCTCC CCGTTGAAGG ACAGTGACGA GGTCCGGTCA 
CTCTGGGTCA AAATTGACTT GACACTTCTG TCCAGGATCC CGGAACACCT GCCCCAGGAG 
CCGGGGGTCT TGAGTGCTCC GGCAGCCAAG GACTCTGAGA GCGCGCCCCC GAGCCACGCA 
TCGGAGGCGC CCGTGGAAAA GACCTTGCCA AAATCCAAGA GGAAACGCAA GTGTGACAAC 
GAAGACGATT ACAGGGAGAT CAAGAAGGCC CCGGGAGAGA GGGAGAGCGC CTCACGACTG 
AGCGCCTCTG CCAACAGTAC TTTGTCTGCA AATCATTGCA GCATGAACAT CAACAGCTTG 
GCAATACCAA TAAATAAAAA TGAAAAAGTG CTCCGGTCGC CCATCTCACC CCTCTCCGAC 
GCATCTAAAC ACAAATTCTC CGGCGAGGAC TTGACTTCCT CCAGCAGATC CAACAGCAAC 
GGTCTGTTCA CCTCCACCTC CTCCAGCAAA AAGCATAAGG CAGAGAGCCA GCTGCAGAGC 
CACGCCGCGG ACCTCACGAA AGCGGCTCAC AACAATTCCG AAAACATTCT CCACAAGTCA 
CGGCCGCAGA CAGAGCCCTG GTCTCCAGGC TCCAACGGCC ACCGGGACTG CAAGAGGCAG 
AAACTCATCT TCGACGATAT GCCACGCAGT GCAGATTATT TTATGCAAGA AGCTAAGCGA 
ATGAAGCATA AAGCAGATGC GATGGTGGAA AAGTTTGGAA AGGCTCTGAA CTATGCAGAA 
GCAGCCTTGT CATTCATTGA GTGTGGAAAT GCCATGGAAC AGGGCCCCAT GGAGTCCAAA 
TCTCCTTACA CCATGTATTC AGAGACAGTT GAGCTCATCA GGTATGCCAT GAGACTAAAA 
ACCCACTCAG GTCCCAATGC CACACCAGAG GATAAACAGC TGGCTGCATT ATGTTACCGA 
TGCCTGGCTC TCCTGTACTG GAGGATGTTT CGACTCAAAA GGGATCACGC TGTAAAGTAT 
TCAAAAGCAC TTATCGACTA TTTCAAGGAG CACTGGGACC CCATCCCCCA TGTCTCCCAA 
TCCGTCCCCC ACCGGCTCCG TGGGCTCTCA GGGGAGCCTG TCCAACAGCA ACGCCCTGTC 
CCCATCCACC ATCGTCAGCA TCCCGCAGCG CATCCACCAG ATGGCGGCCA ATCACGTGAG 
CATCACCAAC AGCATTCTCC ACAGCTACGA CTACTGGGAG ATGGCAGACA ACCTGGCCAA 
GGAAAACAGA GAATTCTTCC ACGACCTGGA CCTGCTCATG GGGCCGGTCA CCCTGCACAG 
CAGCATGGAG CACCTGGTCC AGTACTCCCA GCAGGGCCTG CACTGGCTGC GAAACAGCGC 
CCACCTGGCC TAGACACCTG CCCCCGGCTG CGGCGTGCAC GCATCTCCAC GGATGGCCCA 
GCGTTTCGGG ACACTGTGCT CCCCACGTTC CCGGCCACAG CATTTCTCGA AAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855080.1
CDS	19..4278
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999312.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999312.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGC AGGCAGCCCA GAGAACGGCC 
CTCCATGCTC TGTCTGACAG TGCCGGACTC CAGCCGGCCG ACTGCAGGGC CTCCGCACCC 
TCGAGCAAGG GCAGCAGCAG CGGCAGCAGC AGCAGTGGGA GCAGCTCCTC CAGCGACTCG 
GAGAGCAGCT CGGGGTCCGA CTCGGAGACA GAGAGCAGTT CCAGCGAGAG CGAGGGCAGC 
AAGCCTCCCC ACTACTCCAG TCCCGAGGCG GAACCAGCGT CCTCTAACAA GTGGCAGCTG 
GATAAATGGC TAAACAAAGT GAATCACCAC AAGCCCCCTG TCCTGATCCA AAATGAAAGC 
CACCACGGGC CGGAGAGCAG TCAGTACTAC ACCCCAGTGA AGGAGGACAC GCAGGACTGC 
GGGAAGCTCC CCGACGTCTG CCCGCCCGGC CTGAGAGACA AGGACATCAA GGGCGCCTGC 
AAGGAGGAGC AGAGGCCCAG GACCGCGAAC AAGGCCCCCG GGAGCAAAGG GGTGAAGCAG 
AAGTCCCCGC CCGCGGCCGT GGCCGTGGCC GTGACCGCCG CCGCCGCCCC GCCGCCCGCG 
GTGCCTGGTG CGCCCGCCGA GAGCGCGCCC GCACCTGCCC GGAGGTCCGC GGGCAAGAAG 
CCCACCCGGC GCACCGAGAG GACCTCGGCC GGCGACAGCG GCCACCGGCC CGAGGAGCCC 
GTAGCTGCCG AGGCGCTGGG GGCGAGCACG GGGGTTCCCC CGGAGCCCCC CAAGCCCCGG 
CCTTGCGGCA ACAGCAGGAC CAACCACCGC AAGGAGCTGC GCTCGGCCGT GACCTGCGAG 
AAGCGCCGCA CTCGGGGGCT GAGCCGGATC GTCCCCAAGT CCAAGGAGTT CATCGAGACG 
GAGTCGTCCT CTTCCTCCTC CTCCTCGGAC TCGGACCTGG AGTCGGAGCA GGAGGACTAC 
CCTCTGTCCA AGGCCGTGGC CGCCGCCGCC GCCGCTGCCG CTGCCTCCGG GAACGACCCA 
AGGCTGAAGG AGGCTCCCAG CAGCATCAGC GGCGGCGGCC CCCGCGCTCC CGTGGGCTCC 
ATCAACGCCA GGACCACCAG CGACATTGCC AAGGAGCTGG AGGAGCAGTT CTACACACTG 
GTCCCCTTCG GCCGCAACGA GCTTCTCTCC CCGTTGAAGG ACAGTGACGA GGTCCGGTCA 
CTCTGGGTCA AAATTGACTT GACACTTCTG TCCAGGATCC CGGAACACCT GCCCCAGGAG 
CCGGGGGTCT TGAGTGCTCC GGCAGCCAAG GACTCTGAGA GCGCGCCCCC GAGCCACGCA 
TCGGAGGCGC CCGTGGAAAA GACCTTGCCA AAATCCAAGA GGAAACGCAA GTGTGACAAC 
GAAGACGATT ACAGGGAGAT CAAGAAGGCC CCGGGAGAGA GGGAGAGCGC CTCACGACTG 
AGCGCCTCTG CCAACAGTAC TTTGTCTGCA AATCATTGCA GCATGAACAT CAACAGCTTG 
GCAATACCAA TAAATAAAAA TGAAAAAGTG CTCCGGTCGC CCATCTCACC CCTCTCCGAC 
GCATCTAAAC ACAAATTCTC CGGCGAGGAC TTGACTTCCT CCAGCAGATC CAACAGCAAC 
GGTCTGTTCA CCTCCACCTC CTCCAGCAAA AAGCATAAGG CAGAGAGCCA GCTGCAGAGC 
CACGCCGCGG ACCTCACGAA AGCGGCTCAC AACAATTCCG AAAACATTCT CCACAAGTCA 
CGGCCGCAGA CAGAGCCCTG GTCTCCAGGC TCCAACGGCC ACCGGGACTG CAAGAGGCAG 
AAACTCATCT TCGACGATAT GCCACGCAGT GCAGATTATT TTATGCAAGA AGCTAAGCGA 
ATGAAGCATA AAGCAGATGC GATGGTGGAA AAGTTTGGAA AGGCTCTGAA CTATGCAGAA 
GCAGCCTTGT CATTCATTGA GTGTGGAAAT GCCATGGAAC AGGGCCCCAT GGAGTCCAAA 
TCTCCTTACA CCATGTATTC AGAGACAGTT GAGCTCATCA GGTATGCCAT GAGACTAAAA 
ACCCACTCAG GTCCCAATGC CACACCAGAG GATAAACAGC TGGCTGCATT ATGTTACCGA 
TGCCTGGCTC TCCTGTACTG GAGGATGTTT CGACTCAAAA GGGATCACGC TGTAAAGTAT 
TCAAAAGCAC TTATCGACTA TTTCAAGGAG CACTGGGACC CCATCCCCCA TGTCTCCCAA 
TCCGTCCCCC ACCGGCTCCG TGGGCTCTCA GGGGAGCCTG TCCAACAGCA ACGCCCTGTC 
CCCATCCACC ATCGTCAGCA TCCCGCAGCG CATCCACCAG ATGGCGGCCA ATCACGTGAG 
CATCACCAAC AGCATTCTCC ACAGCTACGA CTACTGGGAG ATGGCAGACA ACCTGGCCAA 
GGAAAACAGA GAATTCTTCC ACGACCTGGA CCTGCTCATG GGGCCGGTCA CCCTGCACAG 
CAGCATGGAG CACCTGGTCC AGTACTCCCA GCAGGGCCTG CACTGGCTGC GAAACAGCGC 
CCACCTGGCC TAGACACCTG CCCCCGGCTG CGGCGTGCAC GCATCTCCAC GGATGGCCCA 
GCGTTTCGGG ACACTGTGCT CCCCACGTTC CCGGCCACAG CATTTCTCGA AAGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999313.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855081.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220734
Clone ID Related Accession (Same CDS sequence)	XM_024999313.1
Accession Version	XM_024999313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGG CAGCCCAGAG AACGGCCCTC 
CATGCTCTGT CTGACAGTGC CGGACTCCAG CCGGCCGACT GCAGGGCCTC CGCACCCTCG 
AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC AGTGGGAGCA GCTCCTCCAG CGACTCGGAG 
AGCAGCTCGG GGTCCGACTC GGAGACAGAG AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG 
CCTCCCCACT ACTCCAGTCC CGAGGCGGAA CCAGCGTCCT CTAACAAGTG GCAGCTGGAT 
AAATGGCTAA ACAAAGTGAA TCACCACAAG CCCCCTGTCC TGATCCAAAA TGAAAGCCAC 
CACGGGCCGG AGAGCAGTCA GTACTACACC CCAGTGAAGG AGGACACGCA GGACTGCGGG 
AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG AGAGACAAGG ACATCAAGGG CGCCTGCAAG 
GAGGAGCAGA GGCCCAGGAC CGCGAACAAG GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG 
TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG 
CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC 
ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC GACAGCGGCC ACCGGCCCGA GGAGCCCGTA 
GCTGCCGAGG CGCTGGGGGC GAGCACGGGG GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT 
TGCGGCAACA GCAGGACCAA CCACCGCAAG GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG 
CGCCGCACTC GGGGGCTGAG CCGGATCGTC CCCAAGTCCA AGGAGTTCAT CGAGACGGAG 
TCGTCCTCTT CCTCCTCCTC CTCGGACTCG GACCTGGAGT CGGAGCAGGA GGACTACCCT 
CTGTCCAAGG CCGTGGCCGC CGCCGCCGCC GCTGCCGCTG CCTCCGGGAA CGACCCAAGG 
CTGAAGGAGG CTCCCAGCAG CATCAGCGGC GGCGGCCCCC GCGCTCCCGT GGGCTCCATC 
AACGCCAGGA CCACCAGCGA CATTGCCAAG GAGCTGGAGG AGCAGTTCTA CACACTGGTC 
CCCTTCGGCC GCAACGAGCT TCTCTCCCCG TTGAAGGACA GTGACGAGGT CCGGTCACTC 
TGGGTCAAAA TTGACTTGAC ACTTCTGTCC AGGATCCCGG AACACCTGCC CCAGGAGCCG 
GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC TCTGAGAGCG CGCCCCCGAG CCACGCATCG 
GAGGCGCCCG TGGAAAAGAC CTTGCCAAAA TCCAAGAGGA AACGCAAGTG TGACAACGAA 
GACGATTACA GGGAGATCAA GAAGGCCCCG GGAGAGAGGG AGAGCGCCTC ACGACTGAGC 
GCCTCTGCCA ACAGTACTTT GTCTGCAAAT CATTGCAGCA TGAACATCAA CAGCTTGGCA 
ATACCAATAA ATAAAAATGA AAAAGTGCTC CGGTCGCCCA TCTCACCCCT CTCCGACGCA 
TCTAAACACA AATTCTCCGG CGAGGACTTG ACTTCCTCCA GCAGATCCAA CAGCAACGGT 
CTGTTCACCT CCACCTCCTC CAGCAAAAAG CATAAGGCAG AGAGCCAGCT GCAGAGCCAC 
GCCGCGGACC TCACGAAAGC GGCTCACAAC AATTCCGAAA ACATTCTCCA CAAGTCACGG 
CCGCAGACAG AGCCCTGGTC TCCAGGCTCC AACGGCCACC GGGACTGCAA GAGGCAGAAA 
CTCATCTTCG ACGATATGCC ACGCAGTGCA GATTATTTTA TGCAAGAAGC TAAGCGAATG 
AAGCATAAAG CAGATGCGAT GGTGGAAAAG TTTGGAAAGG CTCTGAACTA TGCAGAAGCA 
GCCTTGTCAT TCATTGAGTG TGGAAATGCC ATGGAACAGG GCCCCATGGA GTCCAAATCT 
CCTTACACCA TGTATTCAGA GACAGTTGAG CTCATCAGGT ATGCCATGAG ACTAAAAACC 
CACTCAGGTC CCAATGCCAC ACCAGAGGAT AAACAGCTGG CTGCATTATG TTACCGATGC 
CTGGCTCTCC TGTACTGGAG GATGTTTCGA CTCAAAAGGG ATCACGCTGT AAAGTATTCA 
AAAGCACTTA TCGACTATTT CAAGGAGCAC TGGGACCCCA TCCCCCATGT CTCCCAATCC 
GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC AACAGCAACG CCCTGTCCCC 
ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG GCGGCCAATC ACGTGAGCAT 
CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG GCAGACAACC TGGCCAAGGA 
AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG CCGGTCACCC TGCACAGCAG 
CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC TGGCTGCGAA ACAGCGCCCA 
CCTGGCCTAG ACACCTGCCC CCGGCTGCGG CGTGCACGCA TCTCCACGGA TGGCCCAGCG 
TTTCGGGACA CTGTGCTCCC CACGTTCCCG GCCACAGCAT TTCTCGAAAG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855081.1
CDS	19..4275
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999313.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999313.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGG CAGCCCAGAG AACGGCCCTC 
CATGCTCTGT CTGACAGTGC CGGACTCCAG CCGGCCGACT GCAGGGCCTC CGCACCCTCG 
AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC AGTGGGAGCA GCTCCTCCAG CGACTCGGAG 
AGCAGCTCGG GGTCCGACTC GGAGACAGAG AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG 
CCTCCCCACT ACTCCAGTCC CGAGGCGGAA CCAGCGTCCT CTAACAAGTG GCAGCTGGAT 
AAATGGCTAA ACAAAGTGAA TCACCACAAG CCCCCTGTCC TGATCCAAAA TGAAAGCCAC 
CACGGGCCGG AGAGCAGTCA GTACTACACC CCAGTGAAGG AGGACACGCA GGACTGCGGG 
AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG AGAGACAAGG ACATCAAGGG CGCCTGCAAG 
GAGGAGCAGA GGCCCAGGAC CGCGAACAAG GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG 
TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG 
CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC 
ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC GACAGCGGCC ACCGGCCCGA GGAGCCCGTA 
GCTGCCGAGG CGCTGGGGGC GAGCACGGGG GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT 
TGCGGCAACA GCAGGACCAA CCACCGCAAG GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG 
CGCCGCACTC GGGGGCTGAG CCGGATCGTC CCCAAGTCCA AGGAGTTCAT CGAGACGGAG 
TCGTCCTCTT CCTCCTCCTC CTCGGACTCG GACCTGGAGT CGGAGCAGGA GGACTACCCT 
CTGTCCAAGG CCGTGGCCGC CGCCGCCGCC GCTGCCGCTG CCTCCGGGAA CGACCCAAGG 
CTGAAGGAGG CTCCCAGCAG CATCAGCGGC GGCGGCCCCC GCGCTCCCGT GGGCTCCATC 
AACGCCAGGA CCACCAGCGA CATTGCCAAG GAGCTGGAGG AGCAGTTCTA CACACTGGTC 
CCCTTCGGCC GCAACGAGCT TCTCTCCCCG TTGAAGGACA GTGACGAGGT CCGGTCACTC 
TGGGTCAAAA TTGACTTGAC ACTTCTGTCC AGGATCCCGG AACACCTGCC CCAGGAGCCG 
GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC TCTGAGAGCG CGCCCCCGAG CCACGCATCG 
GAGGCGCCCG TGGAAAAGAC CTTGCCAAAA TCCAAGAGGA AACGCAAGTG TGACAACGAA 
GACGATTACA GGGAGATCAA GAAGGCCCCG GGAGAGAGGG AGAGCGCCTC ACGACTGAGC 
GCCTCTGCCA ACAGTACTTT GTCTGCAAAT CATTGCAGCA TGAACATCAA CAGCTTGGCA 
ATACCAATAA ATAAAAATGA AAAAGTGCTC CGGTCGCCCA TCTCACCCCT CTCCGACGCA 
TCTAAACACA AATTCTCCGG CGAGGACTTG ACTTCCTCCA GCAGATCCAA CAGCAACGGT 
CTGTTCACCT CCACCTCCTC CAGCAAAAAG CATAAGGCAG AGAGCCAGCT GCAGAGCCAC 
GCCGCGGACC TCACGAAAGC GGCTCACAAC AATTCCGAAA ACATTCTCCA CAAGTCACGG 
CCGCAGACAG AGCCCTGGTC TCCAGGCTCC AACGGCCACC GGGACTGCAA GAGGCAGAAA 
CTCATCTTCG ACGATATGCC ACGCAGTGCA GATTATTTTA TGCAAGAAGC TAAGCGAATG 
AAGCATAAAG CAGATGCGAT GGTGGAAAAG TTTGGAAAGG CTCTGAACTA TGCAGAAGCA 
GCCTTGTCAT TCATTGAGTG TGGAAATGCC ATGGAACAGG GCCCCATGGA GTCCAAATCT 
CCTTACACCA TGTATTCAGA GACAGTTGAG CTCATCAGGT ATGCCATGAG ACTAAAAACC 
CACTCAGGTC CCAATGCCAC ACCAGAGGAT AAACAGCTGG CTGCATTATG TTACCGATGC 
CTGGCTCTCC TGTACTGGAG GATGTTTCGA CTCAAAAGGG ATCACGCTGT AAAGTATTCA 
AAAGCACTTA TCGACTATTT CAAGGAGCAC TGGGACCCCA TCCCCCATGT CTCCCAATCC 
GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC AACAGCAACG CCCTGTCCCC 
ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG GCGGCCAATC ACGTGAGCAT 
CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG GCAGACAACC TGGCCAAGGA 
AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG CCGGTCACCC TGCACAGCAG 
CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC TGGCTGCGAA ACAGCGCCCA 
CCTGGCCTAG ACACCTGCCC CCGGCTGCGG CGTGCACGCA TCTCCACGGA TGGCCCAGCG 
TTTCGGGACA CTGTGCTCCC CACGTTCCCG GCCACAGCAT TTCTCGAAAG GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999314.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855082.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

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CloneID	OBa220735
Clone ID Related Accession (Same CDS sequence)	XM_024999314.1
Accession Version	XM_024999314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGCAGGC AGCCCAGAGA 
ACGGCCCTCC ATGCTCTGTC TGACAGTGCC GGACTCCAGC CGGCCGACTG CAGGGCCTCC 
GCACCCTCGA GCAAGGGCAG CAGCAGCGGC AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC 
GACTCGGAGA GCAGCTCGGG GTCCGACTCG GAGACAGAGA GCAGTTCCAG CGAGAGCGAG 
GGCAGCAAGC CTCCCCACTA CTCCAGTCCC GAGGCGGAAC CAGCGTCCTC TAACAAGTGG 
CAGCTGGATA AATGGCTAAA CAAAGTGAAT CACCACAAGC CCCCTGTCCT GATCCAAAAT 
GAAAGCCACC ACGGGCCGGA GAGCAGTCAG TACTACACCC CAGTGAAGGA GGACACGCAG 
GACTGCGGGA AGCTCCCCGA CGTCTGCCCG CCCGGCCTGA GAGACAAGGA CATCAAGGGC 
GCCTGCAAGG AGGAGCAGAG GCCCAGGACC GCGAACAAGG CCCCCGGGAG CAAAGGGGTG 
AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG 
CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC 
AAGAAGCCCA CCCGGCGCAC CGAGAGGACC TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG 
GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG AGCACGGGGG TTCCCCCGGA GCCCCCCAAG 
CCCCGGCCTT GCGGCAACAG CAGGACCAAC CACCGCAAGG AGCTGCGCTC GGCCGTGACC 
TGCGAGAAGC GCCGCACTCG GGGGCTGAGC CGGATCGTCC CCAAGTCCAA GGAGTTCATC 
GAGACGGAGT CGTCCTCTTC CTCCTCCTCC TCGGACTCGG ACCTGGAGTC GGAGCAGGAG 
GACTACCCTC TGTCCAAGGC CGTGGCCGCC GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC 
GACCCAAGGC TGAAGGAGGC TCCCAGCAGC ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG 
GGCTCCATCA ACGCCAGGAC CACCAGCGAC ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC 
ACACTGGTCC CCTTCGGCCG CAACGAGCTT CTCTCCCCGT TGAAGGACAG TGACGAGGTC 
CGGTCACTCT GGGTCAAAAT TGACTTGACA CTTCTGTCCA GGATCCCGGA ACACCTGCCC 
CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA GCCAAGGACT CTGAGAGCGC GCCCCCGAGC 
CACGCATCGG AGGCGCCCGT GGAAAAGACC TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT 
GACAACGAAG ACGATTACAG GGAGATCAAG AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA 
CGACTGAGCG CCTCTGCCAA CAGTACTTTG TCTGCAAATC ATTGCAGCAT GAACATCAAC 
AGCTTGGCAA TACCAATAAA TAAAAATGAA AAAGTGCTCC GGTCGCCCAT CTCACCCCTC 
TCCGACGCAT CTAAACACAA ATTCTCCGGC GAGGACTTGA CTTCCTCCAG CAGATCCAAC 
AGCAACGGTC TGTTCACCTC CACCTCCTCC AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG 
CAGAGCCACG CCGCGGACCT CACGAAAGCG GCTCACAACA ATTCCGAAAA CATTCTCCAC 
AAGTCACGGC CGCAGACAGA GCCCTGGTCT CCAGGCTCCA ACGGCCACCG GGACTGCAAG 
AGGCAGAAAC TCATCTTCGA CGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAGCGAATGA AGCATAAAGC AGATGCGATG GTGGAAAAGT TTGGAAAGGC TCTGAACTAT 
GCAGAAGCAG CCTTGTCATT CATTGAGTGT GGAAATGCCA TGGAACAGGG CCCCATGGAG 
TCCAAATCTC CTTACACCAT GTATTCAGAG ACAGTTGAGC TCATCAGGTA TGCCATGAGA 
CTAAAAACCC ACTCAGGTCC CAATGCCACA CCAGAGGATA AACAGCTGGC TGCATTATGT 
TACCGATGCC TGGCTCTCCT GTACTGGAGG ATGTTTCGAC TCAAAAGGGA TCACGCTGTA 
AAGTATTCAA AAGCACTTAT CGACTATTTC AAGGAGCACT GGGACCCCAT CCCCCATGTC 
TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG AGCCTGTCCA ACAGCAACGC 
CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC CACCAGATGG CGGCCAATCA 
CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC TGGGAGATGG CAGACAACCT 
GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG CTCATGGGGC CGGTCACCCT 
GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGCCTGCACT GGCTGCGAAA 
CAGCGCCCAC CTGGCCTAGA CACCTGCCCC CGGCTGCGGC GTGCACGCAT CTCCACGGAT 
GGCCCAGCGT TTCGGGACAC TGTGCTCCCC ACGTTCCCGG CCACAGCATT TCTCGAAAGG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855082.1
CDS	19..4224
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999314.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999314.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGCAGGC AGCCCAGAGA 
ACGGCCCTCC ATGCTCTGTC TGACAGTGCC GGACTCCAGC CGGCCGACTG CAGGGCCTCC 
GCACCCTCGA GCAAGGGCAG CAGCAGCGGC AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC 
GACTCGGAGA GCAGCTCGGG GTCCGACTCG GAGACAGAGA GCAGTTCCAG CGAGAGCGAG 
GGCAGCAAGC CTCCCCACTA CTCCAGTCCC GAGGCGGAAC CAGCGTCCTC TAACAAGTGG 
CAGCTGGATA AATGGCTAAA CAAAGTGAAT CACCACAAGC CCCCTGTCCT GATCCAAAAT 
GAAAGCCACC ACGGGCCGGA GAGCAGTCAG TACTACACCC CAGTGAAGGA GGACACGCAG 
GACTGCGGGA AGCTCCCCGA CGTCTGCCCG CCCGGCCTGA GAGACAAGGA CATCAAGGGC 
GCCTGCAAGG AGGAGCAGAG GCCCAGGACC GCGAACAAGG CCCCCGGGAG CAAAGGGGTG 
AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG 
CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC 
AAGAAGCCCA CCCGGCGCAC CGAGAGGACC TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG 
GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG AGCACGGGGG TTCCCCCGGA GCCCCCCAAG 
CCCCGGCCTT GCGGCAACAG CAGGACCAAC CACCGCAAGG AGCTGCGCTC GGCCGTGACC 
TGCGAGAAGC GCCGCACTCG GGGGCTGAGC CGGATCGTCC CCAAGTCCAA GGAGTTCATC 
GAGACGGAGT CGTCCTCTTC CTCCTCCTCC TCGGACTCGG ACCTGGAGTC GGAGCAGGAG 
GACTACCCTC TGTCCAAGGC CGTGGCCGCC GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC 
GACCCAAGGC TGAAGGAGGC TCCCAGCAGC ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG 
GGCTCCATCA ACGCCAGGAC CACCAGCGAC ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC 
ACACTGGTCC CCTTCGGCCG CAACGAGCTT CTCTCCCCGT TGAAGGACAG TGACGAGGTC 
CGGTCACTCT GGGTCAAAAT TGACTTGACA CTTCTGTCCA GGATCCCGGA ACACCTGCCC 
CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA GCCAAGGACT CTGAGAGCGC GCCCCCGAGC 
CACGCATCGG AGGCGCCCGT GGAAAAGACC TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT 
GACAACGAAG ACGATTACAG GGAGATCAAG AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA 
CGACTGAGCG CCTCTGCCAA CAGTACTTTG TCTGCAAATC ATTGCAGCAT GAACATCAAC 
AGCTTGGCAA TACCAATAAA TAAAAATGAA AAAGTGCTCC GGTCGCCCAT CTCACCCCTC 
TCCGACGCAT CTAAACACAA ATTCTCCGGC GAGGACTTGA CTTCCTCCAG CAGATCCAAC 
AGCAACGGTC TGTTCACCTC CACCTCCTCC AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG 
CAGAGCCACG CCGCGGACCT CACGAAAGCG GCTCACAACA ATTCCGAAAA CATTCTCCAC 
AAGTCACGGC CGCAGACAGA GCCCTGGTCT CCAGGCTCCA ACGGCCACCG GGACTGCAAG 
AGGCAGAAAC TCATCTTCGA CGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAGCGAATGA AGCATAAAGC AGATGCGATG GTGGAAAAGT TTGGAAAGGC TCTGAACTAT 
GCAGAAGCAG CCTTGTCATT CATTGAGTGT GGAAATGCCA TGGAACAGGG CCCCATGGAG 
TCCAAATCTC CTTACACCAT GTATTCAGAG ACAGTTGAGC TCATCAGGTA TGCCATGAGA 
CTAAAAACCC ACTCAGGTCC CAATGCCACA CCAGAGGATA AACAGCTGGC TGCATTATGT 
TACCGATGCC TGGCTCTCCT GTACTGGAGG ATGTTTCGAC TCAAAAGGGA TCACGCTGTA 
AAGTATTCAA AAGCACTTAT CGACTATTTC AAGGAGCACT GGGACCCCAT CCCCCATGTC 
TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG AGCCTGTCCA ACAGCAACGC 
CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC CACCAGATGG CGGCCAATCA 
CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC TGGGAGATGG CAGACAACCT 
GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG CTCATGGGGC CGGTCACCCT 
GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGCCTGCACT GGCTGCGAAA 
CAGCGCCCAC CTGGCCTAGA CACCTGCCCC CGGCTGCGGC GTGCACGCAT CTCCACGGAT 
GGCCCAGCGT TTCGGGACAC TGTGCTCCCC ACGTTCCCGG CCACAGCATT TCTCGAAAGG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999315.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855083.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.

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CloneID	OBa220736
Clone ID Related Accession (Same CDS sequence)	XM_024999315.1
Accession Version	XM_024999315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGC AGGCAGCCCA GAGAACGGCC 
CTCCATGCTC TGTCTGACAG TGCCGGACTC CAGCCGGCCG ACTGCAGGGC CTCCGCACCC 
TCGAGCAAGG GCAGCAGCAG CGGCAGCAGC AGCAGTGGGA GCAGCTCCTC CAGCGACTCG 
GAGAGCAGCT CGGGGTCCGA CTCGGAGACA GAGAGCAGTT CCAGCGAGAG CGAGGGCAGC 
AAGCCTCCCC ACTACTCCAG TCCCGAGGCG GAACCAGCGT CCTCTAACAA GTGGCAGCTG 
GATAAATGGC TAAACAAAGT GAATCACCAC AAGCCCCCTG TCCTGATCCA AAATGAAAGC 
CACCACGGGC CGGAGAGCAG TCAGTACTAC ACCCCAGTGA AGGAGGACAC GCAGGACTGC 
GGGAAGCTCC CCGACGTCTG CCCGCCCGGC CTGAGAGACA AGGACATCAA GGGCGCCTGC 
AAGGAGGAGC AGAGGCCCAG GACCGCGAAC AAGGCCCCCG GGAGCAAAGG GGTGAAGCAG 
AAGTCCCCGC CCGCGGCCGT GGCCGTGGCC GTGACCGCCG CCGCCGCCCC GCCGCCCGCG 
GTGCCTGGTG CGCCCGCCGA GAGCGCGCCC GCACCTGCCC GGAGGTCCGC GGGCAAGAAG 
CCCACCCGGC GCACCGAGAG GACCTCGGCC GGCGACAGCG GCCACCGGCC CGAGGAGCCC 
GTAGCTGCCG AGGCGCTGGG GGCGAGCACG GGGGTTCCCC CGGAGCCCCC CAAGCCCCGG 
CCTTGCGGCA ACAGCAGGAC CAACCACCGC AAGGAGCTGC GCTCGGCCGT GACCTGCGAG 
AAGCGCCGCA CTCGGGGGCT GAGCCGGATC GTCCCCAAGT CCAAGGAGTT CATCGAGACG 
GAGTCGTCCT CTTCCTCCTC CTCCTCGGAC TCGGACCTGG AGTCGGAGCA GGAGGACTAC 
CCTCTGTCCA AGGCCGTGGC CGCCGCCGCC GCCGCTGCCG CTGCCTCCGG GAACGACCCA 
AGGCTGAAGG AGGCTCCCAG CAGCATCAGC GGCGGCGGCC CCCGCGCTCC CGTGGGCTCC 
ATCAACGCCA GGACCACCAG CGACATTGCC AAGGAGCTGG AGGAGCAGTT CTACACACTG 
GTCCCCTTCG GCCGCAACGA GCTTCTCTCC CCGTTGAAGG ACAGTGACGA GGTCCGGTCA 
CTCTGGGTCA AAATTGACTT GACACTTCTG TCCAGGATCC CGGAACACCT GCCCCAGGAG 
CCGGGGGTCT TGAGTGCTCC GGCAGCCAAG GACTCTGAGA GCGCGCCCCC GAGCCACGCA 
TCGGAGGCGC CCGTGGAAAA GACCTTGCCA AAATCCAAGA GGAAACGCAA GTGTGACAAC 
GAAGACGATT ACAGGGAGAT CAAGAAGGCC CCGGGAGAGA GGGAGAGCGC CTCACGACTG 
AGCGCCTCTG CCAACAGTAC TTTGTCTGCA AATCATTGCA GCATGAACAT CAACAGCTTG 
GCAATACCAA TAAATAAAAA TGAAAAAGTG CTCCGGTCGC CCATCTCACC CCTCTCCGAC 
GCATCTAAAC ACAAATTCTC CGGCGAGGAC TTGACTTCCT CCAGCAGATC CAACAGCAAC 
GGTCTGTTCA CCTCCACCTC CTCCAGCAAA AAGCATAAGG CAGAGAGCCA GCTGCAGAGC 
CACGCCGCGG ACCTCACGAA AGCGGCTCAC AACAATTCCG AAAACATTCT CCACAAGTCA 
CGGCCGCAGA CAGAGCCCTG GTCTCCAGGC TCCAACGGCC ACCGGGACTG CAAGAGGCAG 
AAACTCATCT TCGACGATAT GCCACGCAGT GCAGATTATT TTATGCAAGA AGCTAAGCGA 
ATGAAGCATA AAGCAGATGC GATGGTGGAA AAGTTTGGAA AGGCTCTGAA CTATGCAGAA 
GCAGCCTTGT CATTCATTGA GTGTGGAAAT GCCATGGAAC AGGGCCCCAT GGAGTCCAAA 
TCTCCTTACA CCATGTATTC AGAGACAGTT GAGCTCATCA GGTATGCCAT GAGACTAAAA 
ACCCACTCAG GTCCCAATGC CACACCAGAG GATAAACAGC TGGCTGCATT ATGTTACCGA 
TGCCTGGCTC TCCTGTACTG GAGGATGTTT CGACTCAAAA GGGATCACGC TGTAAAGTAT 
TCAAAAGCAC TTATCGACTA TTTCAAGAAT TCATCTAAAG CTGCCCAAGC CCCATCTCCA 
TGGGGGGCCA GTGGAAAGAG CACTGGGACC CCATCCCCCA TGTCTCCCAA TCCGTCCCCC 
ACCGGCTCCG TGGGCTCTCA GGGGAGCCTG TCCAACAGCA ACGCCCTGTC CCCATCCACC 
ATCGTCAGCA TCCCGCAGCG CATCCACCAG ATGGCGGCCA ATCACGTGAG CATCACCAAC 
AGCATTCTCC ACAGCTACGA CTACTGGGAG ATGGCAGACA ACCTGGCCAA GGAAAACAGA 
GAATTCTTCC ACGACCTGGA CCTGCTCATG GGGCCGGTCA CCCTGCACAG CAGCATGGAG 
CACCTGGTCC AGTACTCCCA GCAGGGCCTG CACTGGCTGC GAAACAGCGC CCACCTGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855083.1
CDS	19..4221
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_024999315.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999315.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCCTCTCTG 
GGATGGTTAT CCCCATTTAC AGATAAGGGA AATGATGCAC TGAGAGGAAT GCTAGAAGAT 
GACCTTAAGC TAAGCAGCGA TGAAGAGGAG AATGAACAGC AGGCAGCCCA GAGAACGGCC 
CTCCATGCTC TGTCTGACAG TGCCGGACTC CAGCCGGCCG ACTGCAGGGC CTCCGCACCC 
TCGAGCAAGG GCAGCAGCAG CGGCAGCAGC AGCAGTGGGA GCAGCTCCTC CAGCGACTCG 
GAGAGCAGCT CGGGGTCCGA CTCGGAGACA GAGAGCAGTT CCAGCGAGAG CGAGGGCAGC 
AAGCCTCCCC ACTACTCCAG TCCCGAGGCG GAACCAGCGT CCTCTAACAA GTGGCAGCTG 
GATAAATGGC TAAACAAAGT GAATCACCAC AAGCCCCCTG TCCTGATCCA AAATGAAAGC 
CACCACGGGC CGGAGAGCAG TCAGTACTAC ACCCCAGTGA AGGAGGACAC GCAGGACTGC 
GGGAAGCTCC CCGACGTCTG CCCGCCCGGC CTGAGAGACA AGGACATCAA GGGCGCCTGC 
AAGGAGGAGC AGAGGCCCAG GACCGCGAAC AAGGCCCCCG GGAGCAAAGG GGTGAAGCAG 
AAGTCCCCGC CCGCGGCCGT GGCCGTGGCC GTGACCGCCG CCGCCGCCCC GCCGCCCGCG 
GTGCCTGGTG CGCCCGCCGA GAGCGCGCCC GCACCTGCCC GGAGGTCCGC GGGCAAGAAG 
CCCACCCGGC GCACCGAGAG GACCTCGGCC GGCGACAGCG GCCACCGGCC CGAGGAGCCC 
GTAGCTGCCG AGGCGCTGGG GGCGAGCACG GGGGTTCCCC CGGAGCCCCC CAAGCCCCGG 
CCTTGCGGCA ACAGCAGGAC CAACCACCGC AAGGAGCTGC GCTCGGCCGT GACCTGCGAG 
AAGCGCCGCA CTCGGGGGCT GAGCCGGATC GTCCCCAAGT CCAAGGAGTT CATCGAGACG 
GAGTCGTCCT CTTCCTCCTC CTCCTCGGAC TCGGACCTGG AGTCGGAGCA GGAGGACTAC 
CCTCTGTCCA AGGCCGTGGC CGCCGCCGCC GCCGCTGCCG CTGCCTCCGG GAACGACCCA 
AGGCTGAAGG AGGCTCCCAG CAGCATCAGC GGCGGCGGCC CCCGCGCTCC CGTGGGCTCC 
ATCAACGCCA GGACCACCAG CGACATTGCC AAGGAGCTGG AGGAGCAGTT CTACACACTG 
GTCCCCTTCG GCCGCAACGA GCTTCTCTCC CCGTTGAAGG ACAGTGACGA GGTCCGGTCA 
CTCTGGGTCA AAATTGACTT GACACTTCTG TCCAGGATCC CGGAACACCT GCCCCAGGAG 
CCGGGGGTCT TGAGTGCTCC GGCAGCCAAG GACTCTGAGA GCGCGCCCCC GAGCCACGCA 
TCGGAGGCGC CCGTGGAAAA GACCTTGCCA AAATCCAAGA GGAAACGCAA GTGTGACAAC 
GAAGACGATT ACAGGGAGAT CAAGAAGGCC CCGGGAGAGA GGGAGAGCGC CTCACGACTG 
AGCGCCTCTG CCAACAGTAC TTTGTCTGCA AATCATTGCA GCATGAACAT CAACAGCTTG 
GCAATACCAA TAAATAAAAA TGAAAAAGTG CTCCGGTCGC CCATCTCACC CCTCTCCGAC 
GCATCTAAAC ACAAATTCTC CGGCGAGGAC TTGACTTCCT CCAGCAGATC CAACAGCAAC 
GGTCTGTTCA CCTCCACCTC CTCCAGCAAA AAGCATAAGG CAGAGAGCCA GCTGCAGAGC 
CACGCCGCGG ACCTCACGAA AGCGGCTCAC AACAATTCCG AAAACATTCT CCACAAGTCA 
CGGCCGCAGA CAGAGCCCTG GTCTCCAGGC TCCAACGGCC ACCGGGACTG CAAGAGGCAG 
AAACTCATCT TCGACGATAT GCCACGCAGT GCAGATTATT TTATGCAAGA AGCTAAGCGA 
ATGAAGCATA AAGCAGATGC GATGGTGGAA AAGTTTGGAA AGGCTCTGAA CTATGCAGAA 
GCAGCCTTGT CATTCATTGA GTGTGGAAAT GCCATGGAAC AGGGCCCCAT GGAGTCCAAA 
TCTCCTTACA CCATGTATTC AGAGACAGTT GAGCTCATCA GGTATGCCAT GAGACTAAAA 
ACCCACTCAG GTCCCAATGC CACACCAGAG GATAAACAGC TGGCTGCATT ATGTTACCGA 
TGCCTGGCTC TCCTGTACTG GAGGATGTTT CGACTCAAAA GGGATCACGC TGTAAAGTAT 
TCAAAAGCAC TTATCGACTA TTTCAAGAAT TCATCTAAAG CTGCCCAAGC CCCATCTCCA 
TGGGGGGCCA GTGGAAAGAG CACTGGGACC CCATCCCCCA TGTCTCCCAA TCCGTCCCCC 
ACCGGCTCCG TGGGCTCTCA GGGGAGCCTG TCCAACAGCA ACGCCCTGTC CCCATCCACC 
ATCGTCAGCA TCCCGCAGCG CATCCACCAG ATGGCGGCCA ATCACGTGAG CATCACCAAC 
AGCATTCTCC ACAGCTACGA CTACTGGGAG ATGGCAGACA ACCTGGCCAA GGAAAACAGA 
GAATTCTTCC ACGACCTGGA CCTGCTCATG GGGCCGGTCA CCCTGCACAG CAGCATGGAG 
CACCTGGTCC AGTACTCCCA GCAGGGCCTG CACTGGCTGC GAAACAGCGC CCACCTGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999316.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855084.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa220737
Clone ID Related Accession (Same CDS sequence)	XM_024999316.1
Accession Version	XM_024999316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGGCAGC CCAGAGAACG 
GCCCTCCATG CTCTGTCTGA CAGTGCCGGA CTCCAGCCGG CCGACTGCAG GGCCTCCGCA 
CCCTCGAGCA AGGGCAGCAG CAGCGGCAGC AGCAGCAGTG GGAGCAGCTC CTCCAGCGAC 
TCGGAGAGCA GCTCGGGGTC CGACTCGGAG ACAGAGAGCA GTTCCAGCGA GAGCGAGGGC 
AGCAAGCCTC CCCACTACTC CAGTCCCGAG GCGGAACCAG CGTCCTCTAA CAAGTGGCAG 
CTGGATAAAT GGCTAAACAA AGTGAATCAC CACAAGCCCC CTGTCCTGAT CCAAAATGAA 
AGCCACCACG GGCCGGAGAG CAGTCAGTAC TACACCCCAG TGAAGGAGGA CACGCAGGAC 
TGCGGGAAGC TCCCCGACGT CTGCCCGCCC GGCCTGAGAG ACAAGGACAT CAAGGGCGCC 
TGCAAGGAGG AGCAGAGGCC CAGGACCGCG AACAAGGCCC CCGGGAGCAA AGGGGTGAAG 
CAGAAGTCCC CGCCCGCGGC CGTGGCCGTG GCCGTGACCG CCGCCGCCGC CCCGCCGCCC 
GCGGTGCCTG GTGCGCCCGC CGAGAGCGCG CCCGCACCTG CCCGGAGGTC CGCGGGCAAG 
AAGCCCACCC GGCGCACCGA GAGGACCTCG GCCGGCGACA GCGGCCACCG GCCCGAGGAG 
CCCGTAGCTG CCGAGGCGCT GGGGGCGAGC ACGGGGGTTC CCCCGGAGCC CCCCAAGCCC 
CGGCCTTGCG GCAACAGCAG GACCAACCAC CGCAAGGAGC TGCGCTCGGC CGTGACCTGC 
GAGAAGCGCC GCACTCGGGG GCTGAGCCGG ATCGTCCCCA AGTCCAAGGA GTTCATCGAG 
ACGGAGTCGT CCTCTTCCTC CTCCTCCTCG GACTCGGACC TGGAGTCGGA GCAGGAGGAC 
TACCCTCTGT CCAAGGCCGT GGCCGCCGCC GCCGCCGCTG CCGCTGCCTC CGGGAACGAC 
CCAAGGCTGA AGGAGGCTCC CAGCAGCATC AGCGGCGGCG GCCCCCGCGC TCCCGTGGGC 
TCCATCAACG CCAGGACCAC CAGCGACATT GCCAAGGAGC TGGAGGAGCA GTTCTACACA 
CTGGTCCCCT TCGGCCGCAA CGAGCTTCTC TCCCCGTTGA AGGACAGTGA CGAGGTCCGG 
TCACTCTGGG TCAAAATTGA CTTGACACTT CTGTCCAGGA TCCCGGAACA CCTGCCCCAG 
GAGCCGGGGG TCTTGAGTGC TCCGGCAGCC AAGGACTCTG AGAGCGCGCC CCCGAGCCAC 
GCATCGGAGG CGCCCGTGGA AAAGACCTTG CCAAAATCCA AGAGGAAACG CAAGTGTGAC 
AACGAAGACG ATTACAGGGA GATCAAGAAG GCCCCGGGAG AGAGGGAGAG CGCCTCACGA 
CTGAGCGCCT CTGCCAACAG TACTTTGTCT GCAAATCATT GCAGCATGAA CATCAACAGC 
TTGGCAATAC CAATAAATAA AAATGAAAAA GTGCTCCGGT CGCCCATCTC ACCCCTCTCC 
GACGCATCTA AACACAAATT CTCCGGCGAG GACTTGACTT CCTCCAGCAG ATCCAACAGC 
AACGGTCTGT TCACCTCCAC CTCCTCCAGC AAAAAGCATA AGGCAGAGAG CCAGCTGCAG 
AGCCACGCCG CGGACCTCAC GAAAGCGGCT CACAACAATT CCGAAAACAT TCTCCACAAG 
TCACGGCCGC AGACAGAGCC CTGGTCTCCA GGCTCCAACG GCCACCGGGA CTGCAAGAGG 
CAGAAACTCA TCTTCGACGA TATGCCACGC AGTGCAGATT ATTTTATGCA AGAAGCTAAG 
CGAATGAAGC ATAAAGCAGA TGCGATGGTG GAAAAGTTTG GAAAGGCTCT GAACTATGCA 
GAAGCAGCCT TGTCATTCAT TGAGTGTGGA AATGCCATGG AACAGGGCCC CATGGAGTCC 
AAATCTCCTT ACACCATGTA TTCAGAGACA GTTGAGCTCA TCAGGTATGC CATGAGACTA 
AAAACCCACT CAGGTCCCAA TGCCACACCA GAGGATAAAC AGCTGGCTGC ATTATGTTAC 
CGATGCCTGG CTCTCCTGTA CTGGAGGATG TTTCGACTCA AAAGGGATCA CGCTGTAAAG 
TATTCAAAAG CACTTATCGA CTATTTCAAG GAGCACTGGG ACCCCATCCC CCATGTCTCC 
CAATCCGTCC CCCACCGGCT CCGTGGGCTC TCAGGGGAGC CTGTCCAACA GCAACGCCCT 
GTCCCCATCC ACCATCGTCA GCATCCCGCA GCGCATCCAC CAGATGGCGG CCAATCACGT 
GAGCATCACC AACAGCATTC TCCACAGCTA CGACTACTGG GAGATGGCAG ACAACCTGGC 
CAAGGAAAAC AGAGAATTCT TCCACGACCT GGACCTGCTC ATGGGGCCGG TCACCCTGCA 
CAGCAGCATG GAGCACCTGG TCCAGTACTC CCAGCAGGGC CTGCACTGGC TGCGAAACAG 
CGCCCACCTG GCCTAGACAC CTGCCCCCGG CTGCGGCGTG CACGCATCTC CACGGATGGC 
CCAGCGTTTC GGGACACTGT GCTCCCCACG TTCCCGGCCA CAGCATTTCT CGAAAGGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855084.1
CDS	19..4221
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999316.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999316.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGGCAGC CCAGAGAACG 
GCCCTCCATG CTCTGTCTGA CAGTGCCGGA CTCCAGCCGG CCGACTGCAG GGCCTCCGCA 
CCCTCGAGCA AGGGCAGCAG CAGCGGCAGC AGCAGCAGTG GGAGCAGCTC CTCCAGCGAC 
TCGGAGAGCA GCTCGGGGTC CGACTCGGAG ACAGAGAGCA GTTCCAGCGA GAGCGAGGGC 
AGCAAGCCTC CCCACTACTC CAGTCCCGAG GCGGAACCAG CGTCCTCTAA CAAGTGGCAG 
CTGGATAAAT GGCTAAACAA AGTGAATCAC CACAAGCCCC CTGTCCTGAT CCAAAATGAA 
AGCCACCACG GGCCGGAGAG CAGTCAGTAC TACACCCCAG TGAAGGAGGA CACGCAGGAC 
TGCGGGAAGC TCCCCGACGT CTGCCCGCCC GGCCTGAGAG ACAAGGACAT CAAGGGCGCC 
TGCAAGGAGG AGCAGAGGCC CAGGACCGCG AACAAGGCCC CCGGGAGCAA AGGGGTGAAG 
CAGAAGTCCC CGCCCGCGGC CGTGGCCGTG GCCGTGACCG CCGCCGCCGC CCCGCCGCCC 
GCGGTGCCTG GTGCGCCCGC CGAGAGCGCG CCCGCACCTG CCCGGAGGTC CGCGGGCAAG 
AAGCCCACCC GGCGCACCGA GAGGACCTCG GCCGGCGACA GCGGCCACCG GCCCGAGGAG 
CCCGTAGCTG CCGAGGCGCT GGGGGCGAGC ACGGGGGTTC CCCCGGAGCC CCCCAAGCCC 
CGGCCTTGCG GCAACAGCAG GACCAACCAC CGCAAGGAGC TGCGCTCGGC CGTGACCTGC 
GAGAAGCGCC GCACTCGGGG GCTGAGCCGG ATCGTCCCCA AGTCCAAGGA GTTCATCGAG 
ACGGAGTCGT CCTCTTCCTC CTCCTCCTCG GACTCGGACC TGGAGTCGGA GCAGGAGGAC 
TACCCTCTGT CCAAGGCCGT GGCCGCCGCC GCCGCCGCTG CCGCTGCCTC CGGGAACGAC 
CCAAGGCTGA AGGAGGCTCC CAGCAGCATC AGCGGCGGCG GCCCCCGCGC TCCCGTGGGC 
TCCATCAACG CCAGGACCAC CAGCGACATT GCCAAGGAGC TGGAGGAGCA GTTCTACACA 
CTGGTCCCCT TCGGCCGCAA CGAGCTTCTC TCCCCGTTGA AGGACAGTGA CGAGGTCCGG 
TCACTCTGGG TCAAAATTGA CTTGACACTT CTGTCCAGGA TCCCGGAACA CCTGCCCCAG 
GAGCCGGGGG TCTTGAGTGC TCCGGCAGCC AAGGACTCTG AGAGCGCGCC CCCGAGCCAC 
GCATCGGAGG CGCCCGTGGA AAAGACCTTG CCAAAATCCA AGAGGAAACG CAAGTGTGAC 
AACGAAGACG ATTACAGGGA GATCAAGAAG GCCCCGGGAG AGAGGGAGAG CGCCTCACGA 
CTGAGCGCCT CTGCCAACAG TACTTTGTCT GCAAATCATT GCAGCATGAA CATCAACAGC 
TTGGCAATAC CAATAAATAA AAATGAAAAA GTGCTCCGGT CGCCCATCTC ACCCCTCTCC 
GACGCATCTA AACACAAATT CTCCGGCGAG GACTTGACTT CCTCCAGCAG ATCCAACAGC 
AACGGTCTGT TCACCTCCAC CTCCTCCAGC AAAAAGCATA AGGCAGAGAG CCAGCTGCAG 
AGCCACGCCG CGGACCTCAC GAAAGCGGCT CACAACAATT CCGAAAACAT TCTCCACAAG 
TCACGGCCGC AGACAGAGCC CTGGTCTCCA GGCTCCAACG GCCACCGGGA CTGCAAGAGG 
CAGAAACTCA TCTTCGACGA TATGCCACGC AGTGCAGATT ATTTTATGCA AGAAGCTAAG 
CGAATGAAGC ATAAAGCAGA TGCGATGGTG GAAAAGTTTG GAAAGGCTCT GAACTATGCA 
GAAGCAGCCT TGTCATTCAT TGAGTGTGGA AATGCCATGG AACAGGGCCC CATGGAGTCC 
AAATCTCCTT ACACCATGTA TTCAGAGACA GTTGAGCTCA TCAGGTATGC CATGAGACTA 
AAAACCCACT CAGGTCCCAA TGCCACACCA GAGGATAAAC AGCTGGCTGC ATTATGTTAC 
CGATGCCTGG CTCTCCTGTA CTGGAGGATG TTTCGACTCA AAAGGGATCA CGCTGTAAAG 
TATTCAAAAG CACTTATCGA CTATTTCAAG GAGCACTGGG ACCCCATCCC CCATGTCTCC 
CAATCCGTCC CCCACCGGCT CCGTGGGCTC TCAGGGGAGC CTGTCCAACA GCAACGCCCT 
GTCCCCATCC ACCATCGTCA GCATCCCGCA GCGCATCCAC CAGATGGCGG CCAATCACGT 
GAGCATCACC AACAGCATTC TCCACAGCTA CGACTACTGG GAGATGGCAG ACAACCTGGC 
CAAGGAAAAC AGAGAATTCT TCCACGACCT GGACCTGCTC ATGGGGCCGG TCACCCTGCA 
CAGCAGCATG GAGCACCTGG TCCAGTACTC CCAGCAGGGC CTGCACTGGC TGCGAAACAG 
CGCCCACCTG GCCTAGACAC CTGCCCCCGG CTGCGGCGTG CACGCATCTC CACGGATGGC 
CCAGCGTTTC GGGACACTGT GCTCCCCACG TTCCCGGCCA CAGCATTTCT CGAAAGGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999317.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855085.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa220738
Clone ID Related Accession (Same CDS sequence)	XM_024999317.1
Accession Version	XM_024999317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGTGTCTAC GAACCGGATA GAAACGTGTT ACGGAGGAAA 
GAACGAGAAA GAAGAAACCA GGAAGCTCAG CAGGACGATG GCGCGTTTAA TTCGAGTTAC 
TCCCTCTTTA GTGAACCCTA CAAGACTAAC AAGGGGGATG AGCTCTCCAA CCGGCTCCAG 
AACACTTTAG GCAATTACGA TGAAATGAAA GACTTTCTCA CGGATCGATC AAACCAGAGT 
CATCTTGTGG GTGTTCCCAA ACCTGGGGTT CCTCAGGCTC CCGTGACCAA GATTGATGAA 
CATTTTGTGG CAGATTCAAG AGCCCAGACT CAGCCAGCAT CCGTCTGTAG CACCACATCC 
TCCACCCCAG CAGCCGTCCC TGGGCAGCAG AGTAAGCGGG GTGCCATGGG CTGGCAGAAG 
GCTGGGCACC CGCCGTCCGA TGGCCAACAG AGAGCAACAC AACAGGGCAC TCTCAGGACC 
TTGCTTGGCG ATGGCGTTGG CAGACAGCAG CCGCGGACCA AACAGGTGTG CAATGTCGAG 
GTGGGTCTTC AGACCCAGGA CAGGCCCCCT GCTATGGGAG CCAAGCACAG CGGCAATGGC 
CACTGCGTGC AGAACTTTCC TCCCTCCCTG GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG 
CCTACTGCCT ATGTGCGGCC CATGGATGGC CAAGATCAGG CGCCCGATGA GTCTCCGAAG 
CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC TGCTTGTACA GGGGTGTCCC GGCCACCAAG 
TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC TCCAAGTTCA GCATCCCCAA ACAAGGAGAG 
GAGAGTCGAT CTGGAGAAAC CAACAGCTGT GTTGAAGAAA TCATTCGGGA GATGACCTGG 
CCTCCAGCAC TTGCTGCTAT TCAACCACCT GGCAAAGTGG AACCAAGCAA ATTTCCATTT 
CCAAATAAGG ACTCTCAGCT TGCCTCCTCT GGACACAATA ATCCAAAGAA AGGTGATGCC 
GAGCCAGAGA GTCCAGACAA TGGCACATCG AACACATCCT CTCTGGGATG GTTATCCCCA 
TTTACAGATA AGGGAAATGA TGCACTGAGA GGAATGCTAG AAGATGACCT TAAGCTAAGC 
AGCGATGAAG AGGAGAATGA ACAGCAGGCA GCCCAGAGAA CGGCCCTCCA TGCTCTGTCT 
GACAGTGCCG GACTCCAGCC GGCCGACTGC AGGGCCTCCG CACCCTCGAG CAAGGGCAGC 
AGCAGCGGCA GCAGCAGCAG TGGGAGCAGC TCCTCCAGCG ACTCGGAGAG CAGCTCGGGG 
TCCGACTCGG AGACAGAGAG CAGTTCCAGC GAGAGCGAGG GCAGCAAGCC TCCCCACTAC 
TCCAGTCCCG AGGCGGAACC AGCGTCCTCT AACAAGTGGC AGCTGGATAA ATGGCTAAAC 
AAAGTGAATC ACCACAAGCC CCCTGTCCTG ATCCAAAATG AAAGCCACCA CGGGCCGGAG 
AGCAGTCAGT ACTACACCCC AGTGAAGGAG GACACGCAGG ACTGCGGGAA GCTCCCCGAC 
GTCTGCCCGC CCGGCCTGAG AGACAAGGAC ATCAAGGGCG CCTGCAAGGA GGAGCAGAGG 
CCCAGGACCG CGAACAAGGC CCCCGGGAGC AAAGGGGTGA AGCAGAAGTC CCCGCCCGCG 
GCCGTGGCCG TGGCCGTGAC CGCCGCCGCC GCCCCGCCGC CCGCGGTGCC TGGTGCGCCC 
GCCGAGAGCG CGCCCGCACC TGCCCGGAGG TCCGCGGGCA AGAAGCCCAC CCGGCGCACC 
GAGAGGACCT CGGCCGGCGA CAGCGGCCAC CGGCCCGAGG AGCCCGTAGC TGCCGAGGCG 
CTGGGGGCGA GCACGGGGGT TCCCCCGGAG CCCCCCAAGC CCCGGCCTTG CGGCAACAGC 
AGGACCAACC ACCGCAAGGA GCTGCGCTCG GCCGTGACCT GCGAGAAGCG CCGCACTCGG 
GGGCTGAGCC GGATCGTCCC CAAGTCCAAG GAGTTCATCG AGACGGAGTC GTCCTCTTCC 
TCCTCCTCCT CGGACTCGGA CCTGGAGTCG GAGCAGGAGG ACTACCCTCT GTCCAAGGCC 
GTGGCCGCCG CCGCCGCCGC TGCCGCTGCC TCCGGGAACG ACCCAAGGCT GAAGGAGGCT 
CCCAGCAGCA TCAGCGGCGG CGGCCCCCGC GCTCCCGTGG GCTCCATCAA CGCCAGGACC 
ACCAGCGACA TTGCCAAGGA GCTGGAGGAG CAGTTCTACA CACTGGTCCC CTTCGGCCGC 
AACGAGCTTC TCTCCCCGTT GAAGGACAGT GACGAGGTCC GGTCACTCTG GGTCAAAATT 
GACTTGACAC TTCTGTCCAG GATCCCGGAA CACCTGCCCC AGGAGCCGGG GGTCTTGAGT 
GCTCCGGCAG CCAAGGACTC TGAGAGCGCG CCCCCGAGCC ACGCATCGGA GGCGCCCGTG 
GAAAAGACCT TGCCAAAATC CAAGAGGAAA CGCAAGTGTG ACAACGAAGA CGATTACAGG 
GAGATCAAGA AGGCCCCGGG AGAGAGGGAG AGCGCCTCAC GACTGAGCGC CTCTGCCAAC 
AGTACTTTGT CTGCAAATCA TTGCAGCATG AACATCAACA GCTTGGCAAT ACCAATAAAT 
AAAAATGAAA AAGTGCTCCG GTCGCCCATC TCACCCCTCT CCGACGCATC TAAACACAAA 
TTCTCCGGCG AGGACTTGAC TTCCTCCAGC AGATCCAACA GCAACGGTCT GTTCACCTCC 
ACCTCCTCCA GCAAAAAGCA TAAGGCAGAG AGCCAGCTGC AGAGCCACGC CGCGGACCTC 
ACGAAAGCGG CTCACAACAA TTCCGAAAAC ATTCTCCACA AGTCACGGCC GCAGACAGAG 
CCCTGGTCTC CAGGCTCCAA CGGCCACCGG GACTGCAAGA GGCAGAAACT CATCTTCGAC 
GATATGCCAC GCAGTGCAGA TTATTTTATG CAAGAAGCTA AGCGAATGAA GCATAAAGCA 
GATGCGATGG TGGAAAAGTT TGGAAAGGCT CTGAACTATG CAGAAGCAGC CTTGTCATTC 
ATTGAGTGTG GAAATGCCAT GGAACAGGGC CCCATGGAGT CCAAATCTCC TTACACCATG 
TATTCAGAGA CAGTTGAGCT CATCAGGTAT GCCATGAGAC TAAAAACCCA CTCAGGTCCC 
AATGCCACAC CAGAGGATAA ACAGCTGGCT GCATTATGTT ACCGATGCCT GGCTCTCCTG 
TACTGGAGGA TGTTTCGACT CAAAAGGGAT CACGCTGTAA AGTATTCAAA AGCACTTATC 
GACTATTTCA AGGAGCACTG GGACCCCATC CCCCATGTCT CCCAATCCGT CCCCCACCGG 
CTCCGTGGGC TCTCAGGGGA GCCTGTCCAA CAGCAACGCC CTGTCCCCAT CCACCATCGT 
CAGCATCCCG CAGCGCATCC ACCAGATGGC GGCCAATCAC GTGAGCATCA CCAACAGCAT 
TCTCCACAGC TACGACTACT GGGAGATGGC AGACAACCTG GCCAAGGAAA ACAGAGAATT 
CTTCCACGAC CTGGACCTGC TCATGGGGCC GGTCACCCTG CACAGCAGCA TGGAGCACCT 
GGTCCAGTAC TCCCAGCAGG GCCTGCACTG GCTGCGAAAC AGCGCCCACC TGGCCTAGAC 
ACCTGCCCCC GGCTGCGGCG TGCACGCATC TCCACGGATG GCCCAGCGTT TCGGGACACT 
GTGCTCCCCA CGTTCCCGGC CACAGCATTT CTCGAAAGGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855085.1
CDS	19..4203
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLCVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999317.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999317.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGTGTCTAC GAACCGGATA GAAACGTGTT ACGGAGGAAA 
GAACGAGAAA GAAGAAACCA GGAAGCTCAG CAGGACGATG GCGCGTTTAA TTCGAGTTAC 
TCCCTCTTTA GTGAACCCTA CAAGACTAAC AAGGGGGATG AGCTCTCCAA CCGGCTCCAG 
AACACTTTAG GCAATTACGA TGAAATGAAA GACTTTCTCA CGGATCGATC AAACCAGAGT 
CATCTTGTGG GTGTTCCCAA ACCTGGGGTT CCTCAGGCTC CCGTGACCAA GATTGATGAA 
CATTTTGTGG CAGATTCAAG AGCCCAGACT CAGCCAGCAT CCGTCTGTAG CACCACATCC 
TCCACCCCAG CAGCCGTCCC TGGGCAGCAG AGTAAGCGGG GTGCCATGGG CTGGCAGAAG 
GCTGGGCACC CGCCGTCCGA TGGCCAACAG AGAGCAACAC AACAGGGCAC TCTCAGGACC 
TTGCTTGGCG ATGGCGTTGG CAGACAGCAG CCGCGGACCA AACAGGTGTG CAATGTCGAG 
GTGGGTCTTC AGACCCAGGA CAGGCCCCCT GCTATGGGAG CCAAGCACAG CGGCAATGGC 
CACTGCGTGC AGAACTTTCC TCCCTCCCTG GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG 
CCTACTGCCT ATGTGCGGCC CATGGATGGC CAAGATCAGG CGCCCGATGA GTCTCCGAAG 
CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC TGCTTGTACA GGGGTGTCCC GGCCACCAAG 
TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC TCCAAGTTCA GCATCCCCAA ACAAGGAGAG 
GAGAGTCGAT CTGGAGAAAC CAACAGCTGT GTTGAAGAAA TCATTCGGGA GATGACCTGG 
CCTCCAGCAC TTGCTGCTAT TCAACCACCT GGCAAAGTGG AACCAAGCAA ATTTCCATTT 
CCAAATAAGG ACTCTCAGCT TGCCTCCTCT GGACACAATA ATCCAAAGAA AGGTGATGCC 
GAGCCAGAGA GTCCAGACAA TGGCACATCG AACACATCCT CTCTGGGATG GTTATCCCCA 
TTTACAGATA AGGGAAATGA TGCACTGAGA GGAATGCTAG AAGATGACCT TAAGCTAAGC 
AGCGATGAAG AGGAGAATGA ACAGCAGGCA GCCCAGAGAA CGGCCCTCCA TGCTCTGTCT 
GACAGTGCCG GACTCCAGCC GGCCGACTGC AGGGCCTCCG CACCCTCGAG CAAGGGCAGC 
AGCAGCGGCA GCAGCAGCAG TGGGAGCAGC TCCTCCAGCG ACTCGGAGAG CAGCTCGGGG 
TCCGACTCGG AGACAGAGAG CAGTTCCAGC GAGAGCGAGG GCAGCAAGCC TCCCCACTAC 
TCCAGTCCCG AGGCGGAACC AGCGTCCTCT AACAAGTGGC AGCTGGATAA ATGGCTAAAC 
AAAGTGAATC ACCACAAGCC CCCTGTCCTG ATCCAAAATG AAAGCCACCA CGGGCCGGAG 
AGCAGTCAGT ACTACACCCC AGTGAAGGAG GACACGCAGG ACTGCGGGAA GCTCCCCGAC 
GTCTGCCCGC CCGGCCTGAG AGACAAGGAC ATCAAGGGCG CCTGCAAGGA GGAGCAGAGG 
CCCAGGACCG CGAACAAGGC CCCCGGGAGC AAAGGGGTGA AGCAGAAGTC CCCGCCCGCG 
GCCGTGGCCG TGGCCGTGAC CGCCGCCGCC GCCCCGCCGC CCGCGGTGCC TGGTGCGCCC 
GCCGAGAGCG CGCCCGCACC TGCCCGGAGG TCCGCGGGCA AGAAGCCCAC CCGGCGCACC 
GAGAGGACCT CGGCCGGCGA CAGCGGCCAC CGGCCCGAGG AGCCCGTAGC TGCCGAGGCG 
CTGGGGGCGA GCACGGGGGT TCCCCCGGAG CCCCCCAAGC CCCGGCCTTG CGGCAACAGC 
AGGACCAACC ACCGCAAGGA GCTGCGCTCG GCCGTGACCT GCGAGAAGCG CCGCACTCGG 
GGGCTGAGCC GGATCGTCCC CAAGTCCAAG GAGTTCATCG AGACGGAGTC GTCCTCTTCC 
TCCTCCTCCT CGGACTCGGA CCTGGAGTCG GAGCAGGAGG ACTACCCTCT GTCCAAGGCC 
GTGGCCGCCG CCGCCGCCGC TGCCGCTGCC TCCGGGAACG ACCCAAGGCT GAAGGAGGCT 
CCCAGCAGCA TCAGCGGCGG CGGCCCCCGC GCTCCCGTGG GCTCCATCAA CGCCAGGACC 
ACCAGCGACA TTGCCAAGGA GCTGGAGGAG CAGTTCTACA CACTGGTCCC CTTCGGCCGC 
AACGAGCTTC TCTCCCCGTT GAAGGACAGT GACGAGGTCC GGTCACTCTG GGTCAAAATT 
GACTTGACAC TTCTGTCCAG GATCCCGGAA CACCTGCCCC AGGAGCCGGG GGTCTTGAGT 
GCTCCGGCAG CCAAGGACTC TGAGAGCGCG CCCCCGAGCC ACGCATCGGA GGCGCCCGTG 
GAAAAGACCT TGCCAAAATC CAAGAGGAAA CGCAAGTGTG ACAACGAAGA CGATTACAGG 
GAGATCAAGA AGGCCCCGGG AGAGAGGGAG AGCGCCTCAC GACTGAGCGC CTCTGCCAAC 
AGTACTTTGT CTGCAAATCA TTGCAGCATG AACATCAACA GCTTGGCAAT ACCAATAAAT 
AAAAATGAAA AAGTGCTCCG GTCGCCCATC TCACCCCTCT CCGACGCATC TAAACACAAA 
TTCTCCGGCG AGGACTTGAC TTCCTCCAGC AGATCCAACA GCAACGGTCT GTTCACCTCC 
ACCTCCTCCA GCAAAAAGCA TAAGGCAGAG AGCCAGCTGC AGAGCCACGC CGCGGACCTC 
ACGAAAGCGG CTCACAACAA TTCCGAAAAC ATTCTCCACA AGTCACGGCC GCAGACAGAG 
CCCTGGTCTC CAGGCTCCAA CGGCCACCGG GACTGCAAGA GGCAGAAACT CATCTTCGAC 
GATATGCCAC GCAGTGCAGA TTATTTTATG CAAGAAGCTA AGCGAATGAA GCATAAAGCA 
GATGCGATGG TGGAAAAGTT TGGAAAGGCT CTGAACTATG CAGAAGCAGC CTTGTCATTC 
ATTGAGTGTG GAAATGCCAT GGAACAGGGC CCCATGGAGT CCAAATCTCC TTACACCATG 
TATTCAGAGA CAGTTGAGCT CATCAGGTAT GCCATGAGAC TAAAAACCCA CTCAGGTCCC 
AATGCCACAC CAGAGGATAA ACAGCTGGCT GCATTATGTT ACCGATGCCT GGCTCTCCTG 
TACTGGAGGA TGTTTCGACT CAAAAGGGAT CACGCTGTAA AGTATTCAAA AGCACTTATC 
GACTATTTCA AGGAGCACTG GGACCCCATC CCCCATGTCT CCCAATCCGT CCCCCACCGG 
CTCCGTGGGC TCTCAGGGGA GCCTGTCCAA CAGCAACGCC CTGTCCCCAT CCACCATCGT 
CAGCATCCCG CAGCGCATCC ACCAGATGGC GGCCAATCAC GTGAGCATCA CCAACAGCAT 
TCTCCACAGC TACGACTACT GGGAGATGGC AGACAACCTG GCCAAGGAAA ACAGAGAATT 
CTTCCACGAC CTGGACCTGC TCATGGGGCC GGTCACCCTG CACAGCAGCA TGGAGCACCT 
GGTCCAGTAC TCCCAGCAGG GCCTGCACTG GCTGCGAAAC AGCGCCCACC TGGCCTAGAC 
ACCTGCCCCC GGCTGCGGCG TGCACGCATC TCCACGGATG GCCCAGCGTT TCGGGACACT 
GTGCTCCCCA CGTTCCCGGC CACAGCATTT CTCGAAAGGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999318.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855086.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X7, mRNA.

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CloneID	OBa220739
Clone ID Related Accession (Same CDS sequence)	XM_024999318.1
Accession Version	XM_024999318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGTGTCTAC GAACCGGATA GAAACGTGTT ACGGAGGAAA 
GAACGAGAAA GAAGAAACCA GGAAGCTCAG CAGGACGATG GCGCGTTTAA TTCGAGTTAC 
TCCCTCTTTA GTGAACCCTA CAAGACTAAC AAGGGGGATG AGCTCTCCAA CCGGCTCCAG 
AACACTTTAG GCAATTACGA TGAAATGAAA GACTTTCTCA CGGATCGATC AAACCAGAGT 
CATCTTGTGG GTGTTCCCAA ACCTGGGGTT CCTCAGGCTC CCGTGACCAA GATTGATGAA 
CATTTTGTGG CAGATTCAAG AGCCCAGACT CAGCCAGCAT CCGTCTGTAG CACCACATCC 
TCCACCCCAG CAGCCGTCCC TGGGCAGCAG AGTAAGCGGG GTGCCATGGG CTGGCAGAAG 
GCTGGGCACC CGCCGTCCGA TGGCCAACAG AGAGCAACAC AACAGGGCAC TCTCAGGACC 
TTGCTTGGCG ATGGCGTTGG CAGACAGCAG CCGCGGACCA AACAGGTGTG CAATGTCGAG 
GTGGGTCTTC AGACCCAGGA CAGGCCCCCT GCTATGGGAG CCAAGCACAG CGGCAATGGC 
CACTGCGTGC AGAACTTTCC TCCCTCCCTG GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG 
CCTACTGCCT ATGTGCGGCC CATGGATGGC CAAGATCAGG CGCCCGATGA GTCTCCGAAG 
CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC TGCTTGTACA GGGGTGTCCC GGCCACCAAG 
TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC TCCAAGTTCA GCATCCCCAA ACAAGGAGAG 
GAGAGTCGAT CTGGAGAAAC CAACAGCTGT GTTGAAGAAA TCATTCGGGA GATGACCTGG 
CCTCCAGCAC TTGCTGCTAT TCAACCACCT GGCAAAGTGG AACCAAGCAA ATTTCCATTT 
CCAAATAAGG ACTCTCAGCT TGCCTCCTCT GGACACAATA ATCCAAAGAA AGGTGATGCC 
GAGCCAGAGA GTCCAGACAA TGGCACATCG AACACATCCT CTCTGGGATG GTTATCCCCA 
TTTACAGATA AGGGAAATGA TGCACTGAGA GGAATGCTAG AAGATGACCT TAAGCTAAGC 
AGCGATGAAG AGGAGAATGA ACAGGCAGCC CAGAGAACGG CCCTCCATGC TCTGTCTGAC 
AGTGCCGGAC TCCAGCCGGC CGACTGCAGG GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC 
AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC TCCAGCGACT CGGAGAGCAG CTCGGGGTCC 
GACTCGGAGA CAGAGAGCAG TTCCAGCGAG AGCGAGGGCA GCAAGCCTCC CCACTACTCC 
AGTCCCGAGG CGGAACCAGC GTCCTCTAAC AAGTGGCAGC TGGATAAATG GCTAAACAAA 
GTGAATCACC ACAAGCCCCC TGTCCTGATC CAAAATGAAA GCCACCACGG GCCGGAGAGC 
AGTCAGTACT ACACCCCAGT GAAGGAGGAC ACGCAGGACT GCGGGAAGCT CCCCGACGTC 
TGCCCGCCCG GCCTGAGAGA CAAGGACATC AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC 
AGGACCGCGA ACAAGGCCCC CGGGAGCAAA GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC 
GTGGCCGTGG CCGTGACCGC CGCCGCCGCC CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC 
GAGAGCGCGC CCGCACCTGC CCGGAGGTCC GCGGGCAAGA AGCCCACCCG GCGCACCGAG 
AGGACCTCGG CCGGCGACAG CGGCCACCGG CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG 
GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC CCCAAGCCCC GGCCTTGCGG CAACAGCAGG 
ACCAACCACC GCAAGGAGCT GCGCTCGGCC GTGACCTGCG AGAAGCGCCG CACTCGGGGG 
CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG TTCATCGAGA CGGAGTCGTC CTCTTCCTCC 
TCCTCCTCGG ACTCGGACCT GGAGTCGGAG CAGGAGGACT ACCCTCTGTC CAAGGCCGTG 
GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC GGGAACGACC CAAGGCTGAA GGAGGCTCCC 
AGCAGCATCA GCGGCGGCGG CCCCCGCGCT CCCGTGGGCT CCATCAACGC CAGGACCACC 
AGCGACATTG CCAAGGAGCT GGAGGAGCAG TTCTACACAC TGGTCCCCTT CGGCCGCAAC 
GAGCTTCTCT CCCCGTTGAA GGACAGTGAC GAGGTCCGGT CACTCTGGGT CAAAATTGAC 
TTGACACTTC TGTCCAGGAT CCCGGAACAC CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT 
CCGGCAGCCA AGGACTCTGA GAGCGCGCCC CCGAGCCACG CATCGGAGGC GCCCGTGGAA 
AAGACCTTGC CAAAATCCAA GAGGAAACGC AAGTGTGACA ACGAAGACGA TTACAGGGAG 
ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC GCCTCACGAC TGAGCGCCTC TGCCAACAGT 
ACTTTGTCTG CAAATCATTG CAGCATGAAC ATCAACAGCT TGGCAATACC AATAAATAAA 
AATGAAAAAG TGCTCCGGTC GCCCATCTCA CCCCTCTCCG ACGCATCTAA ACACAAATTC 
TCCGGCGAGG ACTTGACTTC CTCCAGCAGA TCCAACAGCA ACGGTCTGTT CACCTCCACC 
TCCTCCAGCA AAAAGCATAA GGCAGAGAGC CAGCTGCAGA GCCACGCCGC GGACCTCACG 
AAAGCGGCTC ACAACAATTC CGAAAACATT CTCCACAAGT CACGGCCGCA GACAGAGCCC 
TGGTCTCCAG GCTCCAACGG CCACCGGGAC TGCAAGAGGC AGAAACTCAT CTTCGACGAT 
ATGCCACGCA GTGCAGATTA TTTTATGCAA GAAGCTAAGC GAATGAAGCA TAAAGCAGAT 
GCGATGGTGG AAAAGTTTGG AAAGGCTCTG AACTATGCAG AAGCAGCCTT GTCATTCATT 
GAGTGTGGAA ATGCCATGGA ACAGGGCCCC ATGGAGTCCA AATCTCCTTA CACCATGTAT 
TCAGAGACAG TTGAGCTCAT CAGGTATGCC ATGAGACTAA AAACCCACTC AGGTCCCAAT 
GCCACACCAG AGGATAAACA GCTGGCTGCA TTATGTTACC GATGCCTGGC TCTCCTGTAC 
TGGAGGATGT TTCGACTCAA AAGGGATCAC GCTGTAAAGT ATTCAAAAGC ACTTATCGAC 
TATTTCAAGG AGCACTGGGA CCCCATCCCC CATGTCTCCC AATCCGTCCC CCACCGGCTC 
CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG TCCCCATCCA CCATCGTCAG 
CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG AGCATCACCA ACAGCATTCT 
CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC AAGGAAAACA GAGAATTCTT 
CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC AGCAGCATGG AGCACCTGGT 
CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC GCCCACCTGG CCTAGACACC 
TGCCCCCGGC TGCGGCGTGC ACGCATCTCC ACGGATGGCC CAGCGTTTCG GGACACTGTG 
CTCCCCACGT TCCCGGCCAC AGCATTTCTC GAAAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855086.1
CDS	19..4200
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLCVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999318.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999318.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGTGTCTAC GAACCGGATA GAAACGTGTT ACGGAGGAAA 
GAACGAGAAA GAAGAAACCA GGAAGCTCAG CAGGACGATG GCGCGTTTAA TTCGAGTTAC 
TCCCTCTTTA GTGAACCCTA CAAGACTAAC AAGGGGGATG AGCTCTCCAA CCGGCTCCAG 
AACACTTTAG GCAATTACGA TGAAATGAAA GACTTTCTCA CGGATCGATC AAACCAGAGT 
CATCTTGTGG GTGTTCCCAA ACCTGGGGTT CCTCAGGCTC CCGTGACCAA GATTGATGAA 
CATTTTGTGG CAGATTCAAG AGCCCAGACT CAGCCAGCAT CCGTCTGTAG CACCACATCC 
TCCACCCCAG CAGCCGTCCC TGGGCAGCAG AGTAAGCGGG GTGCCATGGG CTGGCAGAAG 
GCTGGGCACC CGCCGTCCGA TGGCCAACAG AGAGCAACAC AACAGGGCAC TCTCAGGACC 
TTGCTTGGCG ATGGCGTTGG CAGACAGCAG CCGCGGACCA AACAGGTGTG CAATGTCGAG 
GTGGGTCTTC AGACCCAGGA CAGGCCCCCT GCTATGGGAG CCAAGCACAG CGGCAATGGC 
CACTGCGTGC AGAACTTTCC TCCCTCCCTG GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG 
CCTACTGCCT ATGTGCGGCC CATGGATGGC CAAGATCAGG CGCCCGATGA GTCTCCGAAG 
CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC TGCTTGTACA GGGGTGTCCC GGCCACCAAG 
TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC TCCAAGTTCA GCATCCCCAA ACAAGGAGAG 
GAGAGTCGAT CTGGAGAAAC CAACAGCTGT GTTGAAGAAA TCATTCGGGA GATGACCTGG 
CCTCCAGCAC TTGCTGCTAT TCAACCACCT GGCAAAGTGG AACCAAGCAA ATTTCCATTT 
CCAAATAAGG ACTCTCAGCT TGCCTCCTCT GGACACAATA ATCCAAAGAA AGGTGATGCC 
GAGCCAGAGA GTCCAGACAA TGGCACATCG AACACATCCT CTCTGGGATG GTTATCCCCA 
TTTACAGATA AGGGAAATGA TGCACTGAGA GGAATGCTAG AAGATGACCT TAAGCTAAGC 
AGCGATGAAG AGGAGAATGA ACAGGCAGCC CAGAGAACGG CCCTCCATGC TCTGTCTGAC 
AGTGCCGGAC TCCAGCCGGC CGACTGCAGG GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC 
AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC TCCAGCGACT CGGAGAGCAG CTCGGGGTCC 
GACTCGGAGA CAGAGAGCAG TTCCAGCGAG AGCGAGGGCA GCAAGCCTCC CCACTACTCC 
AGTCCCGAGG CGGAACCAGC GTCCTCTAAC AAGTGGCAGC TGGATAAATG GCTAAACAAA 
GTGAATCACC ACAAGCCCCC TGTCCTGATC CAAAATGAAA GCCACCACGG GCCGGAGAGC 
AGTCAGTACT ACACCCCAGT GAAGGAGGAC ACGCAGGACT GCGGGAAGCT CCCCGACGTC 
TGCCCGCCCG GCCTGAGAGA CAAGGACATC AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC 
AGGACCGCGA ACAAGGCCCC CGGGAGCAAA GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC 
GTGGCCGTGG CCGTGACCGC CGCCGCCGCC CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC 
GAGAGCGCGC CCGCACCTGC CCGGAGGTCC GCGGGCAAGA AGCCCACCCG GCGCACCGAG 
AGGACCTCGG CCGGCGACAG CGGCCACCGG CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG 
GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC CCCAAGCCCC GGCCTTGCGG CAACAGCAGG 
ACCAACCACC GCAAGGAGCT GCGCTCGGCC GTGACCTGCG AGAAGCGCCG CACTCGGGGG 
CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG TTCATCGAGA CGGAGTCGTC CTCTTCCTCC 
TCCTCCTCGG ACTCGGACCT GGAGTCGGAG CAGGAGGACT ACCCTCTGTC CAAGGCCGTG 
GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC GGGAACGACC CAAGGCTGAA GGAGGCTCCC 
AGCAGCATCA GCGGCGGCGG CCCCCGCGCT CCCGTGGGCT CCATCAACGC CAGGACCACC 
AGCGACATTG CCAAGGAGCT GGAGGAGCAG TTCTACACAC TGGTCCCCTT CGGCCGCAAC 
GAGCTTCTCT CCCCGTTGAA GGACAGTGAC GAGGTCCGGT CACTCTGGGT CAAAATTGAC 
TTGACACTTC TGTCCAGGAT CCCGGAACAC CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT 
CCGGCAGCCA AGGACTCTGA GAGCGCGCCC CCGAGCCACG CATCGGAGGC GCCCGTGGAA 
AAGACCTTGC CAAAATCCAA GAGGAAACGC AAGTGTGACA ACGAAGACGA TTACAGGGAG 
ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC GCCTCACGAC TGAGCGCCTC TGCCAACAGT 
ACTTTGTCTG CAAATCATTG CAGCATGAAC ATCAACAGCT TGGCAATACC AATAAATAAA 
AATGAAAAAG TGCTCCGGTC GCCCATCTCA CCCCTCTCCG ACGCATCTAA ACACAAATTC 
TCCGGCGAGG ACTTGACTTC CTCCAGCAGA TCCAACAGCA ACGGTCTGTT CACCTCCACC 
TCCTCCAGCA AAAAGCATAA GGCAGAGAGC CAGCTGCAGA GCCACGCCGC GGACCTCACG 
AAAGCGGCTC ACAACAATTC CGAAAACATT CTCCACAAGT CACGGCCGCA GACAGAGCCC 
TGGTCTCCAG GCTCCAACGG CCACCGGGAC TGCAAGAGGC AGAAACTCAT CTTCGACGAT 
ATGCCACGCA GTGCAGATTA TTTTATGCAA GAAGCTAAGC GAATGAAGCA TAAAGCAGAT 
GCGATGGTGG AAAAGTTTGG AAAGGCTCTG AACTATGCAG AAGCAGCCTT GTCATTCATT 
GAGTGTGGAA ATGCCATGGA ACAGGGCCCC ATGGAGTCCA AATCTCCTTA CACCATGTAT 
TCAGAGACAG TTGAGCTCAT CAGGTATGCC ATGAGACTAA AAACCCACTC AGGTCCCAAT 
GCCACACCAG AGGATAAACA GCTGGCTGCA TTATGTTACC GATGCCTGGC TCTCCTGTAC 
TGGAGGATGT TTCGACTCAA AAGGGATCAC GCTGTAAAGT ATTCAAAAGC ACTTATCGAC 
TATTTCAAGG AGCACTGGGA CCCCATCCCC CATGTCTCCC AATCCGTCCC CCACCGGCTC 
CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG TCCCCATCCA CCATCGTCAG 
CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG AGCATCACCA ACAGCATTCT 
CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC AAGGAAAACA GAGAATTCTT 
CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC AGCAGCATGG AGCACCTGGT 
CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC GCCCACCTGG CCTAGACACC 
TGCCCCCGGC TGCGGCGTGC ACGCATCTCC ACGGATGGCC CAGCGTTTCG GGACACTGTG 
CTCCCCACGT TCCCGGCCAC AGCATTTCTC GAAAGGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_002691147.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691193.3 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X8, mRNA.

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CloneID	OBa220740
Clone ID Related Accession (Same CDS sequence)	XM_002691147.6
Accession Version	XM_002691147.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691147.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGCAGGC AGCCCAGAGA 
ACGGCCCTCC ATGCTCTGTC TGACAGTGCC GGACTCCAGC CGGCCGACTG CAGGGCCTCC 
GCACCCTCGA GCAAGGGCAG CAGCAGCGGC AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC 
GACTCGGAGA GCAGCTCGGG GTCCGACTCG GAGACAGAGA GCAGTTCCAG CGAGAGCGAG 
GGCAGCAAGC CTCCCCACTA CTCCAGTCCC GAGGCGGAAC CAGCGTCCTC TAACAAGTGG 
CAGCTGGATA AATGGCTAAA CAAAGTGAAT CACCACAAGC CCCCTGTCCT GATCCAAAAT 
GAAAGCCACC ACGGGCCGGA GAGCAGTCAG TACTACACCC CAGTGAAGGA GGACACGCAG 
GACTGCGGGA AGCTCCCCGA CGTCTGCCCG CCCGGCCTGA GAGACAAGGA CATCAAGGGC 
GCCTGCAAGG AGGAGCAGAG GCCCAGGACC GCGAACAAGG CCCCCGGGAG CAAAGGGGTG 
AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG 
CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC 
AAGAAGCCCA CCCGGCGCAC CGAGAGGACC TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG 
GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG AGCACGGGGG TTCCCCCGGA GCCCCCCAAG 
CCCCGGCCTT GCGGCAACAG CAGGACCAAC CACCGCAAGG AGCTGCGCTC GGCCGTGACC 
TGCGAGAAGC GCCGCACTCG GGGGCTGAGC CGGATCGTCC CCAAGTCCAA GGAGTTCATC 
GAGACGGAGT CGTCCTCTTC CTCCTCCTCC TCGGACTCGG ACCTGGAGTC GGAGCAGGAG 
GACTACCCTC TGTCCAAGGC CGTGGCCGCC GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC 
GACCCAAGGC TGAAGGAGGC TCCCAGCAGC ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG 
GGCTCCATCA ACGCCAGGAC CACCAGCGAC ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC 
ACACTGGTCC CCTTCGGCCG CAACGAGCTT CTCTCCCCGT TGAAGGACAG TGACGAGGTC 
CGGTCACTCT GGGTCAAAAT TGACTTGACA CTTCTGTCCA GGATCCCGGA ACACCTGCCC 
CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA GCCAAGGACT CTGAGAGCGC GCCCCCGAGC 
CACGCATCGG AGGCGCCCGT GGAAAAGACC TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT 
GACAACGAAG ACGATTACAG GGAGATCAAG AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA 
CGACTGAGCG CCTCTGCCAA CAGTACTTTG TCTGCAAATC ATTGCAGCAT GAACATCAAC 
AGCTTGGCAA TACCAATAAA TAAAAATGAA AAAGTGCTCC GGTCGCCCAT CTCACCCCTC 
TCCGACGCAT CTAAACACAA ATTCTCCGGC GAGGACTTGA CTTCCTCCAG CAGATCCAAC 
AGCAACGGTC TGTTCACCTC CACCTCCTCC AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG 
CAGAGCCACG CCGCGGACCT CACGAAAGCG GCTCACAACA ATTCCGAAAA CATTCTCCAC 
AAGTCACGGC CGCAGACAGA GCCCTGGTCT CCAGGCTCCA ACGGCCACCG GGACTGCAAG 
AGGCAGAAAC TCATCTTCGA CGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAGCGAATGA AGCATAAAGC AGATGCGATG GTGGAAAAGT TTGGAAAGGC TCTGAACTAT 
GCAGAAGCAG CCTTGTCATT CATTGAGTGT GGAAATGCCA TGGAACAGGG CCCCATGGAG 
TCCAAATCTC CTTACACCAT GTATTCAGAG ACAGTTGAGC TCATCAGGTA TGCCATGAGA 
CTAAAAACCC ACTCAGGTCC CAATGCCACA CCAGAGGATA AACAGCTGGC TGCATTATGT 
TACCGATGCC TGGCTCTCCT GTACTGGAGG ATGTTTCGAC TCAAAAGGGA TCACGCTGTA 
AAGTATTCAA AAGCACTTAT CGACTATTTC AAGAATTCAT CTAAAGCTGC CCAAGCCCCA 
TCTCCATGGG GGGCCAGTGG AAAGAGCACT GGGACCCCAT CCCCCATGTC TCCCAATCCG 
TCCCCCACCG GCTCCGTGGG CTCTCAGGGG AGCCTGTCCA ACAGCAACGC CCTGTCCCCA 
TCCACCATCG TCAGCATCCC GCAGCGCATC CACCAGATGG CGGCCAATCA CGTGAGCATC 
ACCAACAGCA TTCTCCACAG CTACGACTAC TGGGAGATGG CAGACAACCT GGCCAAGGAA 
AACAGAGAAT TCTTCCACGA CCTGGACCTG CTCATGGGGC CGGTCACCCT GCACAGCAGC 
ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGCCTGCACT GGCTGCGAAA CAGCGCCCAC 
CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691193.3
CDS	19..4167
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESARAARSSAAAATAATAAAALTPPTMDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_002691147.6
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691147.6

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGCGC GCGGGCGGCC CGATCCTCGG CGGCGGCGGC GACGGCGGCG 
ACGGCGGCGG CGGCGCTGAC ACCTCCCACC ATGGACAGCT TCGACTTAGC CCTGCTCCAG 
GAATGGGACC TCGAGTCGCT GTGGGGTGAA GACATCCTAA ACCAGAGGAA TGACTCTCTA 
GTTGTGGAGT TTCAGTCATC AGCCAGCAGA TGCAGGAGTG TCTACGAACC GGATAGAAAC 
GTGTTACGGA GGAAAGAACG AGAAAGAAGA AACCAGGAAG CTCAGCAGGA CGATGGCGCG 
TTTAATTCGA GTTACTCCCT CTTTAGTGAA CCCTACAAGA CTAACAAGGG GGATGAGCTC 
TCCAACCGGC TCCAGAACAC TTTAGGCAAT TACGATGAAA TGAAAGACTT TCTCACGGAT 
CGATCAAACC AGAGTCATCT TGTGGGTGTT CCCAAACCTG GGGTTCCTCA GGCTCCCGTG 
ACCAAGATTG ATGAACATTT TGTGGCAGAT TCAAGAGCCC AGACTCAGCC AGCATCCGTC 
TGTAGCACCA CATCCTCCAC CCCAGCAGCC GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC 
ATGGGCTGGC AGAAGGCTGG GCACCCGCCG TCCGATGGCC AACAGAGAGC AACACAACAG 
GGCACTCTCA GGACCTTGCT TGGCGATGGC GTTGGCAGAC AGCAGCCGCG GACCAAACAG 
GTGTGCAATG TCGAGGTGGG TCTTCAGACC CAGGACAGGC CCCCTGCTAT GGGAGCCAAG 
CACAGCGGCA ATGGCCACTG CGTGCAGAAC TTTCCTCCCT CCCTGGCTTC GAAACCCAGC 
CTTGTCCAGC AGAAGCCTAC TGCCTATGTG CGGCCCATGG ATGGCCAAGA TCAGGCGCCC 
GATGAGTCTC CGAAGCTAAA GTCGTCTGCA GAGACTGGCG TGCACTGCTT GTACAGGGGT 
GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA GCCAAGGCCA AGCTCTCCAA GTTCAGCATC 
CCCAAACAAG GAGAGGAGAG TCGATCTGGA GAAACCAACA GCTGTGTTGA AGAAATCATT 
CGGGAGATGA CCTGGCCTCC AGCACTTGCT GCTATTCAAC CACCTGGCAA AGTGGAACCA 
AGCAAATTTC CATTTCCAAA TAAGGACTCT CAGCTTGCCT CCTCTGGACA CAATAATCCA 
AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA GACAATGGCA CATCGAACAC ATCAATGCTA 
GAAGATGACC TTAAGCTAAG CAGCGATGAA GAGGAGAATG AACAGCAGGC AGCCCAGAGA 
ACGGCCCTCC ATGCTCTGTC TGACAGTGCC GGACTCCAGC CGGCCGACTG CAGGGCCTCC 
GCACCCTCGA GCAAGGGCAG CAGCAGCGGC AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC 
GACTCGGAGA GCAGCTCGGG GTCCGACTCG GAGACAGAGA GCAGTTCCAG CGAGAGCGAG 
GGCAGCAAGC CTCCCCACTA CTCCAGTCCC GAGGCGGAAC CAGCGTCCTC TAACAAGTGG 
CAGCTGGATA AATGGCTAAA CAAAGTGAAT CACCACAAGC CCCCTGTCCT GATCCAAAAT 
GAAAGCCACC ACGGGCCGGA GAGCAGTCAG TACTACACCC CAGTGAAGGA GGACACGCAG 
GACTGCGGGA AGCTCCCCGA CGTCTGCCCG CCCGGCCTGA GAGACAAGGA CATCAAGGGC 
GCCTGCAAGG AGGAGCAGAG GCCCAGGACC GCGAACAAGG CCCCCGGGAG CAAAGGGGTG 
AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG 
CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC 
AAGAAGCCCA CCCGGCGCAC CGAGAGGACC TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG 
GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG AGCACGGGGG TTCCCCCGGA GCCCCCCAAG 
CCCCGGCCTT GCGGCAACAG CAGGACCAAC CACCGCAAGG AGCTGCGCTC GGCCGTGACC 
TGCGAGAAGC GCCGCACTCG GGGGCTGAGC CGGATCGTCC CCAAGTCCAA GGAGTTCATC 
GAGACGGAGT CGTCCTCTTC CTCCTCCTCC TCGGACTCGG ACCTGGAGTC GGAGCAGGAG 
GACTACCCTC TGTCCAAGGC CGTGGCCGCC GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC 
GACCCAAGGC TGAAGGAGGC TCCCAGCAGC ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG 
GGCTCCATCA ACGCCAGGAC CACCAGCGAC ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC 
ACACTGGTCC CCTTCGGCCG CAACGAGCTT CTCTCCCCGT TGAAGGACAG TGACGAGGTC 
CGGTCACTCT GGGTCAAAAT TGACTTGACA CTTCTGTCCA GGATCCCGGA ACACCTGCCC 
CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA GCCAAGGACT CTGAGAGCGC GCCCCCGAGC 
CACGCATCGG AGGCGCCCGT GGAAAAGACC TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT 
GACAACGAAG ACGATTACAG GGAGATCAAG AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA 
CGACTGAGCG CCTCTGCCAA CAGTACTTTG TCTGCAAATC ATTGCAGCAT GAACATCAAC 
AGCTTGGCAA TACCAATAAA TAAAAATGAA AAAGTGCTCC GGTCGCCCAT CTCACCCCTC 
TCCGACGCAT CTAAACACAA ATTCTCCGGC GAGGACTTGA CTTCCTCCAG CAGATCCAAC 
AGCAACGGTC TGTTCACCTC CACCTCCTCC AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG 
CAGAGCCACG CCGCGGACCT CACGAAAGCG GCTCACAACA ATTCCGAAAA CATTCTCCAC 
AAGTCACGGC CGCAGACAGA GCCCTGGTCT CCAGGCTCCA ACGGCCACCG GGACTGCAAG 
AGGCAGAAAC TCATCTTCGA CGATATGCCA CGCAGTGCAG ATTATTTTAT GCAAGAAGCT 
AAGCGAATGA AGCATAAAGC AGATGCGATG GTGGAAAAGT TTGGAAAGGC TCTGAACTAT 
GCAGAAGCAG CCTTGTCATT CATTGAGTGT GGAAATGCCA TGGAACAGGG CCCCATGGAG 
TCCAAATCTC CTTACACCAT GTATTCAGAG ACAGTTGAGC TCATCAGGTA TGCCATGAGA 
CTAAAAACCC ACTCAGGTCC CAATGCCACA CCAGAGGATA AACAGCTGGC TGCATTATGT 
TACCGATGCC TGGCTCTCCT GTACTGGAGG ATGTTTCGAC TCAAAAGGGA TCACGCTGTA 
AAGTATTCAA AAGCACTTAT CGACTATTTC AAGAATTCAT CTAAAGCTGC CCAAGCCCCA 
TCTCCATGGG GGGCCAGTGG AAAGAGCACT GGGACCCCAT CCCCCATGTC TCCCAATCCG 
TCCCCCACCG GCTCCGTGGG CTCTCAGGGG AGCCTGTCCA ACAGCAACGC CCTGTCCCCA 
TCCACCATCG TCAGCATCCC GCAGCGCATC CACCAGATGG CGGCCAATCA CGTGAGCATC 
ACCAACAGCA TTCTCCACAG CTACGACTAC TGGGAGATGG CAGACAACCT GGCCAAGGAA 
AACAGAGAAT TCTTCCACGA CCTGGACCTG CTCATGGGGC CGGTCACCCT GCACAGCAGC 
ATGGAGCACC TGGTCCAGTA CTCCCAGCAG GGCCTGCACT GGCTGCGAAA CAGCGCCCAC 
CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_024999319.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855087.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa220741
Clone ID Related Accession (Same CDS sequence)	XM_024999319.1
Accession Version	XM_024999319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGTGT CTACGAACCG GATAGAAACG TGTTACGGAG GAAAGAACGA 
GAAAGAAGAA ACCAGGAAGC TCAGCAGGAC GATGGCGCGT TTAATTCGAG TTACTCCCTC 
TTTAGTGAAC CCTACAAGAC TAACAAGGGG GATGAGCTCT CCAACCGGCT CCAGAACACT 
TTAGGCAATT ACGATGAAAT GAAAGACTTT CTCACGGATC GATCAAACCA GAGTCATCTT 
GTGGGTGTTC CCAAACCTGG GGTTCCTCAG GCTCCCGTGA CCAAGATTGA TGAACATTTT 
GTGGCAGATT CAAGAGCCCA GACTCAGCCA GCATCCGTCT GTAGCACCAC ATCCTCCACC 
CCAGCAGCCG TCCCTGGGCA GCAGAGTAAG CGGGGTGCCA TGGGCTGGCA GAAGGCTGGG 
CACCCGCCGT CCGATGGCCA ACAGAGAGCA ACACAACAGG GCACTCTCAG GACCTTGCTT 
GGCGATGGCG TTGGCAGACA GCAGCCGCGG ACCAAACAGG TGTGCAATGT CGAGGTGGGT 
CTTCAGACCC AGGACAGGCC CCCTGCTATG GGAGCCAAGC ACAGCGGCAA TGGCCACTGC 
GTGCAGAACT TTCCTCCCTC CCTGGCTTCG AAACCCAGCC TTGTCCAGCA GAAGCCTACT 
GCCTATGTGC GGCCCATGGA TGGCCAAGAT CAGGCGCCCG ATGAGTCTCC GAAGCTAAAG 
TCGTCTGCAG AGACTGGCGT GCACTGCTTG TACAGGGGTG TCCCGGCCAC CAAGTCAGAG 
TCAGCCAGAG CCAAGGCCAA GCTCTCCAAG TTCAGCATCC CCAAACAAGG AGAGGAGAGT 
CGATCTGGAG AAACCAACAG CTGTGTTGAA GAAATCATTC GGGAGATGAC CTGGCCTCCA 
GCACTTGCTG CTATTCAACC ACCTGGCAAA GTGGAACCAA GCAAATTTCC ATTTCCAAAT 
AAGGACTCTC AGCTTGCCTC CTCTGGACAC AATAATCCAA AGAAAGGTGA TGCCGAGCCA 
GAGAGTCCAG ACAATGGCAC ATCGAACACA TCCTCTCTGG GATGGTTATC CCCATTTACA 
GATAAGGGAA ATGATGCACT GAGAGGAATG CTAGAAGATG ACCTTAAGCT AAGCAGCGAT 
GAAGAGGAGA ATGAACAGCA GGCAGCCCAG AGAACGGCCC TCCATGCTCT GTCTGACAGT 
GCCGGACTCC AGCCGGCCGA CTGCAGGGCC TCCGCACCCT CGAGCAAGGG CAGCAGCAGC 
GGCAGCAGCA GCAGTGGGAG CAGCTCCTCC AGCGACTCGG AGAGCAGCTC GGGGTCCGAC 
TCGGAGACAG AGAGCAGTTC CAGCGAGAGC GAGGGCAGCA AGCCTCCCCA CTACTCCAGT 
CCCGAGGCGG AACCAGCGTC CTCTAACAAG TGGCAGCTGG ATAAATGGCT AAACAAAGTG 
AATCACCACA AGCCCCCTGT CCTGATCCAA AATGAAAGCC ACCACGGGCC GGAGAGCAGT 
CAGTACTACA CCCCAGTGAA GGAGGACACG CAGGACTGCG GGAAGCTCCC CGACGTCTGC 
CCGCCCGGCC TGAGAGACAA GGACATCAAG GGCGCCTGCA AGGAGGAGCA GAGGCCCAGG 
ACCGCGAACA AGGCCCCCGG GAGCAAAGGG GTGAAGCAGA AGTCCCCGCC CGCGGCCGTG 
GCCGTGGCCG TGACCGCCGC CGCCGCCCCG CCGCCCGCGG TGCCTGGTGC GCCCGCCGAG 
AGCGCGCCCG CACCTGCCCG GAGGTCCGCG GGCAAGAAGC CCACCCGGCG CACCGAGAGG 
ACCTCGGCCG GCGACAGCGG CCACCGGCCC GAGGAGCCCG TAGCTGCCGA GGCGCTGGGG 
GCGAGCACGG GGGTTCCCCC GGAGCCCCCC AAGCCCCGGC CTTGCGGCAA CAGCAGGACC 
AACCACCGCA AGGAGCTGCG CTCGGCCGTG ACCTGCGAGA AGCGCCGCAC TCGGGGGCTG 
AGCCGGATCG TCCCCAAGTC CAAGGAGTTC ATCGAGACGG AGTCGTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCGGAGCAG GAGGACTACC CTCTGTCCAA GGCCGTGGCC 
GCCGCCGCCG CCGCTGCCGC TGCCTCCGGG AACGACCCAA GGCTGAAGGA GGCTCCCAGC 
AGCATCAGCG GCGGCGGCCC CCGCGCTCCC GTGGGCTCCA TCAACGCCAG GACCACCAGC 
GACATTGCCA AGGAGCTGGA GGAGCAGTTC TACACACTGG TCCCCTTCGG CCGCAACGAG 
CTTCTCTCCC CGTTGAAGGA CAGTGACGAG GTCCGGTCAC TCTGGGTCAA AATTGACTTG 
ACACTTCTGT CCAGGATCCC GGAACACCTG CCCCAGGAGC CGGGGGTCTT GAGTGCTCCG 
GCAGCCAAGG ACTCTGAGAG CGCGCCCCCG AGCCACGCAT CGGAGGCGCC CGTGGAAAAG 
ACCTTGCCAA AATCCAAGAG GAAACGCAAG TGTGACAACG AAGACGATTA CAGGGAGATC 
AAGAAGGCCC CGGGAGAGAG GGAGAGCGCC TCACGACTGA GCGCCTCTGC CAACAGTACT 
TTGTCTGCAA ATCATTGCAG CATGAACATC AACAGCTTGG CAATACCAAT AAATAAAAAT 
GAAAAAGTGC TCCGGTCGCC CATCTCACCC CTCTCCGACG CATCTAAACA CAAATTCTCC 
GGCGAGGACT TGACTTCCTC CAGCAGATCC AACAGCAACG GTCTGTTCAC CTCCACCTCC 
TCCAGCAAAA AGCATAAGGC AGAGAGCCAG CTGCAGAGCC ACGCCGCGGA CCTCACGAAA 
GCGGCTCACA ACAATTCCGA AAACATTCTC CACAAGTCAC GGCCGCAGAC AGAGCCCTGG 
TCTCCAGGCT CCAACGGCCA CCGGGACTGC AAGAGGCAGA AACTCATCTT CGACGATATG 
CCACGCAGTG CAGATTATTT TATGCAAGAA GCTAAGCGAA TGAAGCATAA AGCAGATGCG 
ATGGTGGAAA AGTTTGGAAA GGCTCTGAAC TATGCAGAAG CAGCCTTGTC ATTCATTGAG 
TGTGGAAATG CCATGGAACA GGGCCCCATG GAGTCCAAAT CTCCTTACAC CATGTATTCA 
GAGACAGTTG AGCTCATCAG GTATGCCATG AGACTAAAAA CCCACTCAGG TCCCAATGCC 
ACACCAGAGG ATAAACAGCT GGCTGCATTA TGTTACCGAT GCCTGGCTCT CCTGTACTGG 
AGGATGTTTC GACTCAAAAG GGATCACGCT GTAAAGTATT CAAAAGCACT TATCGACTAT 
TTCAAGGAGC ACTGGGACCC CATCCCCCAT GTCTCCCAAT CCGTCCCCCA CCGGCTCCGT 
GGGCTCTCAG GGGAGCCTGT CCAACAGCAA CGCCCTGTCC CCATCCACCA TCGTCAGCAT 
CCCGCAGCGC ATCCACCAGA TGGCGGCCAA TCACGTGAGC ATCACCAACA GCATTCTCCA 
CAGCTACGAC TACTGGGAGA TGGCAGACAA CCTGGCCAAG GAAAACAGAG AATTCTTCCA 
CGACCTGGAC CTGCTCATGG GGCCGGTCAC CCTGCACAGC AGCATGGAGC ACCTGGTCCA 
GTACTCCCAG CAGGGCCTGC ACTGGCTGCG AAACAGCGCC CACCTGGCCT AGACACCTGC 
CCCCGGCTGC GGCGTGCACG CATCTCCACG GATGGCCCAG CGTTTCGGGA CACTGTGCTC 
CCCACGTTCC CGGCCACAGC ATTTCTCGAA AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855087.1
CDS	19..4077
Translation	MAPQKGARAAGSSLLVALALLWVRGGWLHAWWGNAIGWFSHSPPHFHILYQHLHTSVVTTCTLPVYTESAIQKACQGEGSCTLSLRRKTLNNLIWEKKKKSVCVESVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKEHWDPIPHVSQSVPHRLRGLSGEPVQQQRPVPIHHRQHPAAHPPDGGQSREHHQQHSPQLRLLGDGRQPGQGKQRILPRPGPAHGAGHPAQQHGAPGPVLPAGPALAAKQRPPGLDTCPRLRRARISTDGPAFRDTVLPTFPATAFLERR

Target ORF information:

RefSeq Version	XM_024999319.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999319.1

ORF Insert Sequence:

ATGGCCCCCC AGAAGGGGGC CCGGGCTGCT GGTTCCAGCC TTCTGGTCGC CCTTGCTCTG 
CTGTGGGTGA GAGGAGGCTG GCTGCATGCC TGGTGGGGAA ACGCCATTGG CTGGTTTAGT 
CACTCGCCTC CACACTTTCA CATCCTCTAT CAACACCTGC ACACTTCAGT GGTGACCACA 
TGCACTCTTC CTGTTTATAC GGAAAGTGCC ATTCAGAAAG CTTGCCAGGG AGAAGGGAGC 
TGCACCCTCA GCCTCAGAAG GAAGACGTTG AACAACTTAA TTTGGGAGAA AAAGAAAAAG 
AGCGTGTGTG TTGAGAGTGT CTACGAACCG GATAGAAACG TGTTACGGAG GAAAGAACGA 
GAAAGAAGAA ACCAGGAAGC TCAGCAGGAC GATGGCGCGT TTAATTCGAG TTACTCCCTC 
TTTAGTGAAC CCTACAAGAC TAACAAGGGG GATGAGCTCT CCAACCGGCT CCAGAACACT 
TTAGGCAATT ACGATGAAAT GAAAGACTTT CTCACGGATC GATCAAACCA GAGTCATCTT 
GTGGGTGTTC CCAAACCTGG GGTTCCTCAG GCTCCCGTGA CCAAGATTGA TGAACATTTT 
GTGGCAGATT CAAGAGCCCA GACTCAGCCA GCATCCGTCT GTAGCACCAC ATCCTCCACC 
CCAGCAGCCG TCCCTGGGCA GCAGAGTAAG CGGGGTGCCA TGGGCTGGCA GAAGGCTGGG 
CACCCGCCGT CCGATGGCCA ACAGAGAGCA ACACAACAGG GCACTCTCAG GACCTTGCTT 
GGCGATGGCG TTGGCAGACA GCAGCCGCGG ACCAAACAGG TGTGCAATGT CGAGGTGGGT 
CTTCAGACCC AGGACAGGCC CCCTGCTATG GGAGCCAAGC ACAGCGGCAA TGGCCACTGC 
GTGCAGAACT TTCCTCCCTC CCTGGCTTCG AAACCCAGCC TTGTCCAGCA GAAGCCTACT 
GCCTATGTGC GGCCCATGGA TGGCCAAGAT CAGGCGCCCG ATGAGTCTCC GAAGCTAAAG 
TCGTCTGCAG AGACTGGCGT GCACTGCTTG TACAGGGGTG TCCCGGCCAC CAAGTCAGAG 
TCAGCCAGAG CCAAGGCCAA GCTCTCCAAG TTCAGCATCC CCAAACAAGG AGAGGAGAGT 
CGATCTGGAG AAACCAACAG CTGTGTTGAA GAAATCATTC GGGAGATGAC CTGGCCTCCA 
GCACTTGCTG CTATTCAACC ACCTGGCAAA GTGGAACCAA GCAAATTTCC ATTTCCAAAT 
AAGGACTCTC AGCTTGCCTC CTCTGGACAC AATAATCCAA AGAAAGGTGA TGCCGAGCCA 
GAGAGTCCAG ACAATGGCAC ATCGAACACA TCCTCTCTGG GATGGTTATC CCCATTTACA 
GATAAGGGAA ATGATGCACT GAGAGGAATG CTAGAAGATG ACCTTAAGCT AAGCAGCGAT 
GAAGAGGAGA ATGAACAGCA GGCAGCCCAG AGAACGGCCC TCCATGCTCT GTCTGACAGT 
GCCGGACTCC AGCCGGCCGA CTGCAGGGCC TCCGCACCCT CGAGCAAGGG CAGCAGCAGC 
GGCAGCAGCA GCAGTGGGAG CAGCTCCTCC AGCGACTCGG AGAGCAGCTC GGGGTCCGAC 
TCGGAGACAG AGAGCAGTTC CAGCGAGAGC GAGGGCAGCA AGCCTCCCCA CTACTCCAGT 
CCCGAGGCGG AACCAGCGTC CTCTAACAAG TGGCAGCTGG ATAAATGGCT AAACAAAGTG 
AATCACCACA AGCCCCCTGT CCTGATCCAA AATGAAAGCC ACCACGGGCC GGAGAGCAGT 
CAGTACTACA CCCCAGTGAA GGAGGACACG CAGGACTGCG GGAAGCTCCC CGACGTCTGC 
CCGCCCGGCC TGAGAGACAA GGACATCAAG GGCGCCTGCA AGGAGGAGCA GAGGCCCAGG 
ACCGCGAACA AGGCCCCCGG GAGCAAAGGG GTGAAGCAGA AGTCCCCGCC CGCGGCCGTG 
GCCGTGGCCG TGACCGCCGC CGCCGCCCCG CCGCCCGCGG TGCCTGGTGC GCCCGCCGAG 
AGCGCGCCCG CACCTGCCCG GAGGTCCGCG GGCAAGAAGC CCACCCGGCG CACCGAGAGG 
ACCTCGGCCG GCGACAGCGG CCACCGGCCC GAGGAGCCCG TAGCTGCCGA GGCGCTGGGG 
GCGAGCACGG GGGTTCCCCC GGAGCCCCCC AAGCCCCGGC CTTGCGGCAA CAGCAGGACC 
AACCACCGCA AGGAGCTGCG CTCGGCCGTG ACCTGCGAGA AGCGCCGCAC TCGGGGGCTG 
AGCCGGATCG TCCCCAAGTC CAAGGAGTTC ATCGAGACGG AGTCGTCCTC TTCCTCCTCC 
TCCTCGGACT CGGACCTGGA GTCGGAGCAG GAGGACTACC CTCTGTCCAA GGCCGTGGCC 
GCCGCCGCCG CCGCTGCCGC TGCCTCCGGG AACGACCCAA GGCTGAAGGA GGCTCCCAGC 
AGCATCAGCG GCGGCGGCCC CCGCGCTCCC GTGGGCTCCA TCAACGCCAG GACCACCAGC 
GACATTGCCA AGGAGCTGGA GGAGCAGTTC TACACACTGG TCCCCTTCGG CCGCAACGAG 
CTTCTCTCCC CGTTGAAGGA CAGTGACGAG GTCCGGTCAC TCTGGGTCAA AATTGACTTG 
ACACTTCTGT CCAGGATCCC GGAACACCTG CCCCAGGAGC CGGGGGTCTT GAGTGCTCCG 
GCAGCCAAGG ACTCTGAGAG CGCGCCCCCG AGCCACGCAT CGGAGGCGCC CGTGGAAAAG 
ACCTTGCCAA AATCCAAGAG GAAACGCAAG TGTGACAACG AAGACGATTA CAGGGAGATC 
AAGAAGGCCC CGGGAGAGAG GGAGAGCGCC TCACGACTGA GCGCCTCTGC CAACAGTACT 
TTGTCTGCAA ATCATTGCAG CATGAACATC AACAGCTTGG CAATACCAAT AAATAAAAAT 
GAAAAAGTGC TCCGGTCGCC CATCTCACCC CTCTCCGACG CATCTAAACA CAAATTCTCC 
GGCGAGGACT TGACTTCCTC CAGCAGATCC AACAGCAACG GTCTGTTCAC CTCCACCTCC 
TCCAGCAAAA AGCATAAGGC AGAGAGCCAG CTGCAGAGCC ACGCCGCGGA CCTCACGAAA 
GCGGCTCACA ACAATTCCGA AAACATTCTC CACAAGTCAC GGCCGCAGAC AGAGCCCTGG 
TCTCCAGGCT CCAACGGCCA CCGGGACTGC AAGAGGCAGA AACTCATCTT CGACGATATG 
CCACGCAGTG CAGATTATTT TATGCAAGAA GCTAAGCGAA TGAAGCATAA AGCAGATGCG 
ATGGTGGAAA AGTTTGGAAA GGCTCTGAAC TATGCAGAAG CAGCCTTGTC ATTCATTGAG 
TGTGGAAATG CCATGGAACA GGGCCCCATG GAGTCCAAAT CTCCTTACAC CATGTATTCA 
GAGACAGTTG AGCTCATCAG GTATGCCATG AGACTAAAAA CCCACTCAGG TCCCAATGCC 
ACACCAGAGG ATAAACAGCT GGCTGCATTA TGTTACCGAT GCCTGGCTCT CCTGTACTGG 
AGGATGTTTC GACTCAAAAG GGATCACGCT GTAAAGTATT CAAAAGCACT TATCGACTAT 
TTCAAGGAGC ACTGGGACCC CATCCCCCAT GTCTCCCAAT CCGTCCCCCA CCGGCTCCGT 
GGGCTCTCAG GGGAGCCTGT CCAACAGCAA CGCCCTGTCC CCATCCACCA TCGTCAGCAT 
CCCGCAGCGC ATCCACCAGA TGGCGGCCAA TCACGTGAGC ATCACCAACA GCATTCTCCA 
CAGCTACGAC TACTGGGAGA TGGCAGACAA CCTGGCCAAG GAAAACAGAG AATTCTTCCA 
CGACCTGGAC CTGCTCATGG GGCCGGTCAC CCTGCACAGC AGCATGGAGC ACCTGGTCCA 
GTACTCCCAG CAGGGCCTGC ACTGGCTGCG AAACAGCGCC CACCTGGCCT AGACACCTGC 
CCCCGGCTGC GGCGTGCACG CATCTCCACG GATGGCCCAG CGTTTCGGGA CACTGTGCTC 
CCCACGTTCC CGGCCACAGC ATTTCTCGAA AGGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_002700703.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700749.2 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

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CloneID	OBa58183
Clone ID Related Accession (Same CDS sequence)	XM_002700703.5 , XM_002691147.5
Accession Version	XM_002700703.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700703.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCAATGCTA GAAGATGACC TTAAGCTAAG CAGCGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACGGCCCTCC ATGCTCTGTC TGACAGTGCC 
GGACTCCAGC CGGCCGACTG CAGGGCCTCC GCACCCTCGA GCAAGGGCAG CAGCAGCGGC 
AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC GACTCGGAGA GCAGCTCGGG GTCCGACTCG 
GAGACAGAGA GCAGTTCCAG CGAGAGCGAG GGCAGCAAGC CTCCCCACTA CTCCAGTCCC 
GAGGCGGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGATA AATGGCTAAA CAAAGTGAAT 
CACCACAAGC CCCCTGTCCT GATCCAAAAT GAAAGCCACC ACGGGCCGGA GAGCAGTCAG 
TACTACACCC CAGTGAAGGA GGACACGCAG GACTGCGGGA AGCTCCCCGA CGTCTGCCCG 
CCCGGCCTGA GAGACAAGGA CATCAAGGGC GCCTGCAAGG AGGAGCAGAG GCCCAGGACC 
GCGAACAAGG CCCCCGGGAG CAAAGGGGTG AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC 
GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC 
GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC AAGAAGCCCA CCCGGCGCAC CGAGAGGACC 
TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG 
AGCACGGGGG TTCCCCCGGA GCCCCCCAAG CCCCGGCCTT GCGGCAACAG CAGGACCAAC 
CACCGCAAGG AGCTGCGCTC GGCCGTGACC TGCGAGAAGC GCCGCACTCG GGGGCTGAGC 
CGGATCGTCC CCAAGTCCAA GGAGTTCATC GAGACGGAGT CGTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC GGAGCAGGAG GACTACCCTC TGTCCAAGGC TGTGGCCGCC 
GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC GACCCAAGGC TGAAGGAGGC TCCCAGCAGC 
ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACACTGGTCC CCTTCGGCCG CAACGAGCTT 
CTCTCCCCGT TGAAGGACAG TGACGAGGTC CGGTCACTCT GGGTCAAAAT TGACTTGACA 
CTTCTGTCCA GGATCCCGGA ACACCTGCCC CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA 
GCCAAGGACT CTGAGAGCGC GCCCCCGAGC CACGCATCCG AGGCGCCGGT GGAAAAGACC 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAACGAAG ACGATTACAG GGAGATCAAG 
AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA CGACTGAGCG CCTCTGCCAA CAGTACTTTG 
TCCGCAAATC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCGCCCAT CTCACCCCTC TCCGACGCAT CTAAACACAA ATTCTCCGGC 
GAGGACTTGA CTTCCTCCAG CAGATCCAAC AGCAACGGTC TGTTCACCTC CACCTCCTCC 
AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG CAGAGCCACG CCGCGGACCT CACGAAAGCG 
GCTCACAACA ATTCCGAAAA CATTCTCCAC AAGTCACGGC CGCAGACAGA GCCCTGGTCT 
CCAGGCTCCA ACGGCCACCG GGACTGCAAG AGGCAGAAAC TCATCTTCGA CGATATGCCA 
CGCAGTGCAG ATTATTTTAT GCAAGAAGCT AAGCGAATGA AGCATAAAGC AGATGCGATG 
GTGGAAAAGT TTGGAAAGGC TCTGAACTAT GCAGAAGCAG CCTTGTCATT CATTGAGTGT 
GGAAATGCCA TGGAACAGGG CCCCATGGAG TCCAAATCTC CTTACACCAT GTATTCAGAG 
ACAGTTGAGC TCATCAGGTA TGCCATGAGA CTAAAAACCC ACTCAGGTCC CAATGCCACA 
CCAGAGGATA AACAGCTGGC TGCATTATGT TACCGATGCC TGGCTCTCCT GTACTGGAGG 
ATGTTTCGAC TCAAAAGGGA TCACGCTGTA AAGTATTCAA AAGCACTTAT CGACTATTTC 
AAGAATTCAT CTAAAGCTGC CCAAGCCCCA TCTCCATGGG GGGCCAGTGG AAAGAGCACT 
GGGACCCCAT CCCCCATGTC TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG 
AGCCTGTCCA ACAGCAACGC CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC 
CACCAGATGG CGGCCAATCA CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC 
TGGGAGATGG CAGACAACCT GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG 
CTCATGGGGC CGGTCACCCT GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG 
GGCCTGCACT GGCTGCGAAA CAGCGCCCAC CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700749.2
CDS	230..3988
Translation	MDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_002700703.5
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700703.5

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCAATGCTA GAAGATGACC TTAAGCTAAG CAGCGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACGGCCCTCC ATGCTCTGTC TGACAGTGCC 
GGACTCCAGC CGGCCGACTG CAGGGCCTCC GCACCCTCGA GCAAGGGCAG CAGCAGCGGC 
AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC GACTCGGAGA GCAGCTCGGG GTCCGACTCG 
GAGACAGAGA GCAGTTCCAG CGAGAGCGAG GGCAGCAAGC CTCCCCACTA CTCCAGTCCC 
GAGGCGGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGATA AATGGCTAAA CAAAGTGAAT 
CACCACAAGC CCCCTGTCCT GATCCAAAAT GAAAGCCACC ACGGGCCGGA GAGCAGTCAG 
TACTACACCC CAGTGAAGGA GGACACGCAG GACTGCGGGA AGCTCCCCGA CGTCTGCCCG 
CCCGGCCTGA GAGACAAGGA CATCAAGGGC GCCTGCAAGG AGGAGCAGAG GCCCAGGACC 
GCGAACAAGG CCCCCGGGAG CAAAGGGGTG AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC 
GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC 
GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC AAGAAGCCCA CCCGGCGCAC CGAGAGGACC 
TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG 
AGCACGGGGG TTCCCCCGGA GCCCCCCAAG CCCCGGCCTT GCGGCAACAG CAGGACCAAC 
CACCGCAAGG AGCTGCGCTC GGCCGTGACC TGCGAGAAGC GCCGCACTCG GGGGCTGAGC 
CGGATCGTCC CCAAGTCCAA GGAGTTCATC GAGACGGAGT CGTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC GGAGCAGGAG GACTACCCTC TGTCCAAGGC TGTGGCCGCC 
GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC GACCCAAGGC TGAAGGAGGC TCCCAGCAGC 
ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACACTGGTCC CCTTCGGCCG CAACGAGCTT 
CTCTCCCCGT TGAAGGACAG TGACGAGGTC CGGTCACTCT GGGTCAAAAT TGACTTGACA 
CTTCTGTCCA GGATCCCGGA ACACCTGCCC CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA 
GCCAAGGACT CTGAGAGCGC GCCCCCGAGC CACGCATCCG AGGCGCCGGT GGAAAAGACC 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAACGAAG ACGATTACAG GGAGATCAAG 
AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA CGACTGAGCG CCTCTGCCAA CAGTACTTTG 
TCCGCAAATC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCGCCCAT CTCACCCCTC TCCGACGCAT CTAAACACAA ATTCTCCGGC 
GAGGACTTGA CTTCCTCCAG CAGATCCAAC AGCAACGGTC TGTTCACCTC CACCTCCTCC 
AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG CAGAGCCACG CCGCGGACCT CACGAAAGCG 
GCTCACAACA ATTCCGAAAA CATTCTCCAC AAGTCACGGC CGCAGACAGA GCCCTGGTCT 
CCAGGCTCCA ACGGCCACCG GGACTGCAAG AGGCAGAAAC TCATCTTCGA CGATATGCCA 
CGCAGTGCAG ATTATTTTAT GCAAGAAGCT AAGCGAATGA AGCATAAAGC AGATGCGATG 
GTGGAAAAGT TTGGAAAGGC TCTGAACTAT GCAGAAGCAG CCTTGTCATT CATTGAGTGT 
GGAAATGCCA TGGAACAGGG CCCCATGGAG TCCAAATCTC CTTACACCAT GTATTCAGAG 
ACAGTTGAGC TCATCAGGTA TGCCATGAGA CTAAAAACCC ACTCAGGTCC CAATGCCACA 
CCAGAGGATA AACAGCTGGC TGCATTATGT TACCGATGCC TGGCTCTCCT GTACTGGAGG 
ATGTTTCGAC TCAAAAGGGA TCACGCTGTA AAGTATTCAA AAGCACTTAT CGACTATTTC 
AAGAATTCAT CTAAAGCTGC CCAAGCCCCA TCTCCATGGG GGGCCAGTGG AAAGAGCACT 
GGGACCCCAT CCCCCATGTC TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG 
AGCCTGTCCA ACAGCAACGC CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC 
CACCAGATGG CGGCCAATCA CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC 
TGGGAGATGG CAGACAACCT GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG 
CTCATGGGGC CGGTCACCCT GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG 
GGCCTGCACT GGCTGCGAAA CAGCGCCCAC CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_002691147.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002691193.2 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

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CloneID	OBa58183
Clone ID Related Accession (Same CDS sequence)	XM_002700703.5 , XM_002691147.5
Accession Version	XM_002691147.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691147.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCAATGCTA GAAGATGACC TTAAGCTAAG CAGCGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACGGCCCTCC ATGCTCTGTC TGACAGTGCC 
GGACTCCAGC CGGCCGACTG CAGGGCCTCC GCACCCTCGA GCAAGGGCAG CAGCAGCGGC 
AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC GACTCGGAGA GCAGCTCGGG GTCCGACTCG 
GAGACAGAGA GCAGTTCCAG CGAGAGCGAG GGCAGCAAGC CTCCCCACTA CTCCAGTCCC 
GAGGCGGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGATA AATGGCTAAA CAAAGTGAAT 
CACCACAAGC CCCCTGTCCT GATCCAAAAT GAAAGCCACC ACGGGCCGGA GAGCAGTCAG 
TACTACACCC CAGTGAAGGA GGACACGCAG GACTGCGGGA AGCTCCCCGA CGTCTGCCCG 
CCCGGCCTGA GAGACAAGGA CATCAAGGGC GCCTGCAAGG AGGAGCAGAG GCCCAGGACC 
GCGAACAAGG CCCCCGGGAG CAAAGGGGTG AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC 
GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC 
GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC AAGAAGCCCA CCCGGCGCAC CGAGAGGACC 
TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG 
AGCACGGGGG TTCCCCCGGA GCCCCCCAAG CCCCGGCCTT GCGGCAACAG CAGGACCAAC 
CACCGCAAGG AGCTGCGCTC GGCCGTGACC TGCGAGAAGC GCCGCACTCG GGGGCTGAGC 
CGGATCGTCC CCAAGTCCAA GGAGTTCATC GAGACGGAGT CGTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC GGAGCAGGAG GACTACCCTC TGTCCAAGGC TGTGGCCGCC 
GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC GACCCAAGGC TGAAGGAGGC TCCCAGCAGC 
ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACACTGGTCC CCTTCGGCCG CAACGAGCTT 
CTCTCCCCGT TGAAGGACAG TGACGAGGTC CGGTCACTCT GGGTCAAAAT TGACTTGACA 
CTTCTGTCCA GGATCCCGGA ACACCTGCCC CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA 
GCCAAGGACT CTGAGAGCGC GCCCCCGAGC CACGCATCCG AGGCGCCGGT GGAAAAGACC 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAACGAAG ACGATTACAG GGAGATCAAG 
AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA CGACTGAGCG CCTCTGCCAA CAGTACTTTG 
TCCGCAAATC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCGCCCAT CTCACCCCTC TCCGACGCAT CTAAACACAA ATTCTCCGGC 
GAGGACTTGA CTTCCTCCAG CAGATCCAAC AGCAACGGTC TGTTCACCTC CACCTCCTCC 
AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG CAGAGCCACG CCGCGGACCT CACGAAAGCG 
GCTCACAACA ATTCCGAAAA CATTCTCCAC AAGTCACGGC CGCAGACAGA GCCCTGGTCT 
CCAGGCTCCA ACGGCCACCG GGACTGCAAG AGGCAGAAAC TCATCTTCGA CGATATGCCA 
CGCAGTGCAG ATTATTTTAT GCAAGAAGCT AAGCGAATGA AGCATAAAGC AGATGCGATG 
GTGGAAAAGT TTGGAAAGGC TCTGAACTAT GCAGAAGCAG CCTTGTCATT CATTGAGTGT 
GGAAATGCCA TGGAACAGGG CCCCATGGAG TCCAAATCTC CTTACACCAT GTATTCAGAG 
ACAGTTGAGC TCATCAGGTA TGCCATGAGA CTAAAAACCC ACTCAGGTCC CAATGCCACA 
CCAGAGGATA AACAGCTGGC TGCATTATGT TACCGATGCC TGGCTCTCCT GTACTGGAGG 
ATGTTTCGAC TCAAAAGGGA TCACGCTGTA AAGTATTCAA AAGCACTTAT CGACTATTTC 
AAGAATTCAT CTAAAGCTGC CCAAGCCCCA TCTCCATGGG GGGCCAGTGG AAAGAGCACT 
GGGACCCCAT CCCCCATGTC TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG 
AGCCTGTCCA ACAGCAACGC CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC 
CACCAGATGG CGGCCAATCA CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC 
TGGGAGATGG CAGACAACCT GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG 
CTCATGGGGC CGGTCACCCT GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG 
GGCCTGCACT GGCTGCGAAA CAGCGCCCAC CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002691193.2
CDS	230..3988
Translation	MDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_002691147.5
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002691147.5

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCAATGCTA GAAGATGACC TTAAGCTAAG CAGCGATGAA 
GAGGAGAATG AACAGCAGGC AGCCCAGAGA ACGGCCCTCC ATGCTCTGTC TGACAGTGCC 
GGACTCCAGC CGGCCGACTG CAGGGCCTCC GCACCCTCGA GCAAGGGCAG CAGCAGCGGC 
AGCAGCAGCA GTGGGAGCAG CTCCTCCAGC GACTCGGAGA GCAGCTCGGG GTCCGACTCG 
GAGACAGAGA GCAGTTCCAG CGAGAGCGAG GGCAGCAAGC CTCCCCACTA CTCCAGTCCC 
GAGGCGGAAC CAGCGTCCTC TAACAAGTGG CAGCTGGATA AATGGCTAAA CAAAGTGAAT 
CACCACAAGC CCCCTGTCCT GATCCAAAAT GAAAGCCACC ACGGGCCGGA GAGCAGTCAG 
TACTACACCC CAGTGAAGGA GGACACGCAG GACTGCGGGA AGCTCCCCGA CGTCTGCCCG 
CCCGGCCTGA GAGACAAGGA CATCAAGGGC GCCTGCAAGG AGGAGCAGAG GCCCAGGACC 
GCGAACAAGG CCCCCGGGAG CAAAGGGGTG AAGCAGAAGT CCCCGCCCGC GGCCGTGGCC 
GTGGCCGTGA CCGCCGCCGC CGCCCCGCCG CCCGCGGTGC CTGGTGCGCC CGCCGAGAGC 
GCGCCCGCAC CTGCCCGGAG GTCCGCGGGC AAGAAGCCCA CCCGGCGCAC CGAGAGGACC 
TCGGCCGGCG ACAGCGGCCA CCGGCCCGAG GAGCCCGTAG CTGCCGAGGC GCTGGGGGCG 
AGCACGGGGG TTCCCCCGGA GCCCCCCAAG CCCCGGCCTT GCGGCAACAG CAGGACCAAC 
CACCGCAAGG AGCTGCGCTC GGCCGTGACC TGCGAGAAGC GCCGCACTCG GGGGCTGAGC 
CGGATCGTCC CCAAGTCCAA GGAGTTCATC GAGACGGAGT CGTCCTCTTC CTCCTCCTCC 
TCGGACTCGG ACCTGGAGTC GGAGCAGGAG GACTACCCTC TGTCCAAGGC TGTGGCCGCC 
GCCGCCGCCG CTGCCGCTGC CTCCGGGAAC GACCCAAGGC TGAAGGAGGC TCCCAGCAGC 
ATCAGCGGCG GCGGCCCCCG CGCTCCCGTG GGCTCCATCA ACGCCAGGAC CACCAGCGAC 
ATTGCCAAGG AGCTGGAGGA GCAGTTCTAC ACACTGGTCC CCTTCGGCCG CAACGAGCTT 
CTCTCCCCGT TGAAGGACAG TGACGAGGTC CGGTCACTCT GGGTCAAAAT TGACTTGACA 
CTTCTGTCCA GGATCCCGGA ACACCTGCCC CAGGAGCCGG GGGTCTTGAG TGCTCCGGCA 
GCCAAGGACT CTGAGAGCGC GCCCCCGAGC CACGCATCCG AGGCGCCGGT GGAAAAGACC 
TTGCCAAAAT CCAAGAGGAA ACGCAAGTGT GACAACGAAG ACGATTACAG GGAGATCAAG 
AAGGCCCCGG GAGAGAGGGA GAGCGCCTCA CGACTGAGCG CCTCTGCCAA CAGTACTTTG 
TCCGCAAATC ATTGCAGCAT GAACATCAAC AGCTTGGCAA TACCAATAAA TAAAAATGAA 
AAAGTGCTCC GGTCGCCCAT CTCACCCCTC TCCGACGCAT CTAAACACAA ATTCTCCGGC 
GAGGACTTGA CTTCCTCCAG CAGATCCAAC AGCAACGGTC TGTTCACCTC CACCTCCTCC 
AGCAAAAAGC ATAAGGCAGA GAGCCAGCTG CAGAGCCACG CCGCGGACCT CACGAAAGCG 
GCTCACAACA ATTCCGAAAA CATTCTCCAC AAGTCACGGC CGCAGACAGA GCCCTGGTCT 
CCAGGCTCCA ACGGCCACCG GGACTGCAAG AGGCAGAAAC TCATCTTCGA CGATATGCCA 
CGCAGTGCAG ATTATTTTAT GCAAGAAGCT AAGCGAATGA AGCATAAAGC AGATGCGATG 
GTGGAAAAGT TTGGAAAGGC TCTGAACTAT GCAGAAGCAG CCTTGTCATT CATTGAGTGT 
GGAAATGCCA TGGAACAGGG CCCCATGGAG TCCAAATCTC CTTACACCAT GTATTCAGAG 
ACAGTTGAGC TCATCAGGTA TGCCATGAGA CTAAAAACCC ACTCAGGTCC CAATGCCACA 
CCAGAGGATA AACAGCTGGC TGCATTATGT TACCGATGCC TGGCTCTCCT GTACTGGAGG 
ATGTTTCGAC TCAAAAGGGA TCACGCTGTA AAGTATTCAA AAGCACTTAT CGACTATTTC 
AAGAATTCAT CTAAAGCTGC CCAAGCCCCA TCTCCATGGG GGGCCAGTGG AAAGAGCACT 
GGGACCCCAT CCCCCATGTC TCCCAATCCG TCCCCCACCG GCTCCGTGGG CTCTCAGGGG 
AGCCTGTCCA ACAGCAACGC CCTGTCCCCA TCCACCATCG TCAGCATCCC GCAGCGCATC 
CACCAGATGG CGGCCAATCA CGTGAGCATC ACCAACAGCA TTCTCCACAG CTACGACTAC 
TGGGAGATGG CAGACAACCT GGCCAAGGAA AACAGAGAAT TCTTCCACGA CCTGGACCTG 
CTCATGGGGC CGGTCACCCT GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAGCAG 
GGCCTGCACT GGCTGCGAAA CAGCGCCCAC CTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_010809809.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808111.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

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CloneID	OBa58184
Clone ID Related Accession (Same CDS sequence)	XM_010809809.2 , XM_015465296.1
Accession Version	XM_010809809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCCTCTCTG GGATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC TGAGAGGAAT GCTAGAAGAT GACCTTAAGC TAAGCAGCGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACGGCCCTC CATGCTCTGT CTGACAGTGC CGGACTCCAG 
CCGGCCGACT GCAGGGCCTC CGCACCCTCG AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC 
AGTGGGAGCA GCTCCTCCAG CGACTCGGAG AGCAGCTCGG GGTCCGACTC GGAGACAGAG 
AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG CCTCCCCACT ACTCCAGTCC CGAGGCGGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAT AAATGGCTAA ACAAAGTGAA TCACCACAAG 
CCCCCTGTCC TGATCCAAAA TGAAAGCCAC CACGGGCCGG AGAGCAGTCA GTACTACACC 
CCAGTGAAGG AGGACACGCA GGACTGCGGG AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG 
AGAGACAAGG ACATCAAGGG CGCCTGCAAG GAGGAGCAGA GGCCCAGGAC CGCGAACAAG 
GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG 
ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA 
CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC 
GACAGCGGCC ACCGGCCCGA GGAGCCCGTA GCTGCCGAGG CGCTGGGGGC GAGCACGGGG 
GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT TGCGGCAACA GCAGGACCAA CCACCGCAAG 
GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG CGCCGCACTC GGGGGCTGAG CCGGATCGTC 
CCCAAGTCCA AGGAGTTCAT CGAGACGGAG TCGTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CGGAGCAGGA GGACTACCCT CTGTCCAAGG CTGTGGCCGC CGCCGCCGCC 
GCTGCCGCTG CCTCCGGGAA CGACCCAAGG CTGAAGGAGG CTCCCAGCAG CATCAGCGGC 
GGCGGCCCCC GCGCTCCCGT GGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACACTGGTC CCCTTCGGCC GCAACGAGCT TCTCTCCCCG 
TTGAAGGACA GTGACGAGGT CCGGTCACTC TGGGTCAAAA TTGACTTGAC ACTTCTGTCC 
AGGATCCCGG AACACCTGCC CCAGGAGCCG GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC 
TCTGAGAGCG CGCCCCCGAG CCACGCATCC GAGGCGCCGG TGGAAAAGAC CTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAACGAA GACGATTACA GGGAGATCAA GAAGGCCCCG 
GGAGAGAGGG AGAGCGCCTC ACGACTGAGC GCCTCTGCCA ACAGTACTTT GTCCGCAAAT 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCGCCCA TCTCACCCCT CTCCGACGCA TCTAAACACA AATTCTCCGG CGAGGACTTG 
ACTTCCTCCA GCAGATCCAA CAGCAACGGT CTGTTCACCT CCACCTCCTC CAGCAAAAAG 
CATAAGGCAG AGAGCCAGCT GCAGAGCCAC GCCGCGGACC TCACGAAAGC GGCTCACAAC 
AATTCCGAAA ACATTCTCCA CAAGTCACGG CCGCAGACAG AGCCCTGGTC TCCAGGCTCC 
AACGGCCACC GGGACTGCAA GAGGCAGAAA CTCATCTTCG ACGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAGCGAATG AAGCATAAAG CAGATGCGAT GGTGGAAAAG 
TTTGGAAAGG CTCTGAACTA TGCAGAAGCA GCCTTGTCAT TCATTGAGTG TGGAAATGCC 
ATGGAACAGG GCCCCATGGA GTCCAAATCT CCTTACACCA TGTATTCAGA GACAGTTGAG 
CTCATCAGGT ATGCCATGAG ACTAAAAACC CACTCAGGTC CCAATGCCAC ACCAGAGGAT 
AAACAGCTGG CTGCATTATG TTACCGATGC CTGGCTCTCC TGTACTGGAG GATGTTTCGA 
CTCAAAAGGG ATCACGCTGT AAAGTATTCA AAAGCACTTA TCGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGGGCCAGTG GAAAGAGCAC TGGGACCCCA 
TCCCCCATGT CTCCCAATCC GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC 
AACAGCAACG CCCTGTCCCC ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG 
GCGGCCAATC ACGTGAGCAT CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG 
CCGGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC 
TGGCTGCGAA ACAGCGCCCA CCTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808111.1
CDS	230..4039
Translation	MDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_010809809.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809809.2

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCCTCTCTG GGATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC TGAGAGGAAT GCTAGAAGAT GACCTTAAGC TAAGCAGCGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACGGCCCTC CATGCTCTGT CTGACAGTGC CGGACTCCAG 
CCGGCCGACT GCAGGGCCTC CGCACCCTCG AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC 
AGTGGGAGCA GCTCCTCCAG CGACTCGGAG AGCAGCTCGG GGTCCGACTC GGAGACAGAG 
AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG CCTCCCCACT ACTCCAGTCC CGAGGCGGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAT AAATGGCTAA ACAAAGTGAA TCACCACAAG 
CCCCCTGTCC TGATCCAAAA TGAAAGCCAC CACGGGCCGG AGAGCAGTCA GTACTACACC 
CCAGTGAAGG AGGACACGCA GGACTGCGGG AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG 
AGAGACAAGG ACATCAAGGG CGCCTGCAAG GAGGAGCAGA GGCCCAGGAC CGCGAACAAG 
GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG 
ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA 
CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC 
GACAGCGGCC ACCGGCCCGA GGAGCCCGTA GCTGCCGAGG CGCTGGGGGC GAGCACGGGG 
GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT TGCGGCAACA GCAGGACCAA CCACCGCAAG 
GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG CGCCGCACTC GGGGGCTGAG CCGGATCGTC 
CCCAAGTCCA AGGAGTTCAT CGAGACGGAG TCGTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CGGAGCAGGA GGACTACCCT CTGTCCAAGG CTGTGGCCGC CGCCGCCGCC 
GCTGCCGCTG CCTCCGGGAA CGACCCAAGG CTGAAGGAGG CTCCCAGCAG CATCAGCGGC 
GGCGGCCCCC GCGCTCCCGT GGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACACTGGTC CCCTTCGGCC GCAACGAGCT TCTCTCCCCG 
TTGAAGGACA GTGACGAGGT CCGGTCACTC TGGGTCAAAA TTGACTTGAC ACTTCTGTCC 
AGGATCCCGG AACACCTGCC CCAGGAGCCG GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC 
TCTGAGAGCG CGCCCCCGAG CCACGCATCC GAGGCGCCGG TGGAAAAGAC CTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAACGAA GACGATTACA GGGAGATCAA GAAGGCCCCG 
GGAGAGAGGG AGAGCGCCTC ACGACTGAGC GCCTCTGCCA ACAGTACTTT GTCCGCAAAT 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCGCCCA TCTCACCCCT CTCCGACGCA TCTAAACACA AATTCTCCGG CGAGGACTTG 
ACTTCCTCCA GCAGATCCAA CAGCAACGGT CTGTTCACCT CCACCTCCTC CAGCAAAAAG 
CATAAGGCAG AGAGCCAGCT GCAGAGCCAC GCCGCGGACC TCACGAAAGC GGCTCACAAC 
AATTCCGAAA ACATTCTCCA CAAGTCACGG CCGCAGACAG AGCCCTGGTC TCCAGGCTCC 
AACGGCCACC GGGACTGCAA GAGGCAGAAA CTCATCTTCG ACGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAGCGAATG AAGCATAAAG CAGATGCGAT GGTGGAAAAG 
TTTGGAAAGG CTCTGAACTA TGCAGAAGCA GCCTTGTCAT TCATTGAGTG TGGAAATGCC 
ATGGAACAGG GCCCCATGGA GTCCAAATCT CCTTACACCA TGTATTCAGA GACAGTTGAG 
CTCATCAGGT ATGCCATGAG ACTAAAAACC CACTCAGGTC CCAATGCCAC ACCAGAGGAT 
AAACAGCTGG CTGCATTATG TTACCGATGC CTGGCTCTCC TGTACTGGAG GATGTTTCGA 
CTCAAAAGGG ATCACGCTGT AAAGTATTCA AAAGCACTTA TCGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGGGCCAGTG GAAAGAGCAC TGGGACCCCA 
TCCCCCATGT CTCCCAATCC GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC 
AACAGCAACG CCCTGTCCCC ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG 
GCGGCCAATC ACGTGAGCAT CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG 
CCGGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC 
TGGCTGCGAA ACAGCGCCCA CCTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_015465296.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320782.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

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CloneID	OBa58184
Clone ID Related Accession (Same CDS sequence)	XM_010809809.2 , XM_015465296.1
Accession Version	XM_015465296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCCTCTCTG GGATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC TGAGAGGAAT GCTAGAAGAT GACCTTAAGC TAAGCAGCGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACGGCCCTC CATGCTCTGT CTGACAGTGC CGGACTCCAG 
CCGGCCGACT GCAGGGCCTC CGCACCCTCG AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC 
AGTGGGAGCA GCTCCTCCAG CGACTCGGAG AGCAGCTCGG GGTCCGACTC GGAGACAGAG 
AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG CCTCCCCACT ACTCCAGTCC CGAGGCGGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAT AAATGGCTAA ACAAAGTGAA TCACCACAAG 
CCCCCTGTCC TGATCCAAAA TGAAAGCCAC CACGGGCCGG AGAGCAGTCA GTACTACACC 
CCAGTGAAGG AGGACACGCA GGACTGCGGG AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG 
AGAGACAAGG ACATCAAGGG CGCCTGCAAG GAGGAGCAGA GGCCCAGGAC CGCGAACAAG 
GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG 
ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA 
CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC 
GACAGCGGCC ACCGGCCCGA GGAGCCCGTA GCTGCCGAGG CGCTGGGGGC GAGCACGGGG 
GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT TGCGGCAACA GCAGGACCAA CCACCGCAAG 
GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG CGCCGCACTC GGGGGCTGAG CCGGATCGTC 
CCCAAGTCCA AGGAGTTCAT CGAGACGGAG TCGTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CGGAGCAGGA GGACTACCCT CTGTCCAAGG CTGTGGCCGC CGCCGCCGCC 
GCTGCCGCTG CCTCCGGGAA CGACCCAAGG CTGAAGGAGG CTCCCAGCAG CATCAGCGGC 
GGCGGCCCCC GCGCTCCCGT GGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACACTGGTC CCCTTCGGCC GCAACGAGCT TCTCTCCCCG 
TTGAAGGACA GTGACGAGGT CCGGTCACTC TGGGTCAAAA TTGACTTGAC ACTTCTGTCC 
AGGATCCCGG AACACCTGCC CCAGGAGCCG GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC 
TCTGAGAGCG CGCCCCCGAG CCACGCATCC GAGGCGCCGG TGGAAAAGAC CTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAACGAA GACGATTACA GGGAGATCAA GAAGGCCCCG 
GGAGAGAGGG AGAGCGCCTC ACGACTGAGC GCCTCTGCCA ACAGTACTTT GTCCGCAAAT 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCGCCCA TCTCACCCCT CTCCGACGCA TCTAAACACA AATTCTCCGG CGAGGACTTG 
ACTTCCTCCA GCAGATCCAA CAGCAACGGT CTGTTCACCT CCACCTCCTC CAGCAAAAAG 
CATAAGGCAG AGAGCCAGCT GCAGAGCCAC GCCGCGGACC TCACGAAAGC GGCTCACAAC 
AATTCCGAAA ACATTCTCCA CAAGTCACGG CCGCAGACAG AGCCCTGGTC TCCAGGCTCC 
AACGGCCACC GGGACTGCAA GAGGCAGAAA CTCATCTTCG ACGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAGCGAATG AAGCATAAAG CAGATGCGAT GGTGGAAAAG 
TTTGGAAAGG CTCTGAACTA TGCAGAAGCA GCCTTGTCAT TCATTGAGTG TGGAAATGCC 
ATGGAACAGG GCCCCATGGA GTCCAAATCT CCTTACACCA TGTATTCAGA GACAGTTGAG 
CTCATCAGGT ATGCCATGAG ACTAAAAACC CACTCAGGTC CCAATGCCAC ACCAGAGGAT 
AAACAGCTGG CTGCATTATG TTACCGATGC CTGGCTCTCC TGTACTGGAG GATGTTTCGA 
CTCAAAAGGG ATCACGCTGT AAAGTATTCA AAAGCACTTA TCGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGGGCCAGTG GAAAGAGCAC TGGGACCCCA 
TCCCCCATGT CTCCCAATCC GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC 
AACAGCAACG CCCTGTCCCC ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG 
GCGGCCAATC ACGTGAGCAT CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG 
CCGGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC 
TGGCTGCGAA ACAGCGCCCA CCTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320782.1
CDS	230..4039
Translation	MDSFDLALLQEWDLESLWGEDILNQRNDSLVVEFQSSASRCRSVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_015465296.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465296.1

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGGGGTGAA 
GACATCCTAA ACCAGAGGAA TGACTCTCTA GTTGTGGAGT TTCAGTCATC AGCCAGCAGA 
TGCAGGAGTG TCTACGAACC GGATAGAAAC GTGTTACGGA GGAAAGAACG AGAAAGAAGA 
AACCAGGAAG CTCAGCAGGA CGATGGCGCG TTTAATTCGA GTTACTCCCT CTTTAGTGAA 
CCCTACAAGA CTAACAAGGG GGATGAGCTC TCCAACCGGC TCCAGAACAC TTTAGGCAAT 
TACGATGAAA TGAAAGACTT TCTCACGGAT CGATCAAACC AGAGTCATCT TGTGGGTGTT 
CCCAAACCTG GGGTTCCTCA GGCTCCCGTG ACCAAGATTG ATGAACATTT TGTGGCAGAT 
TCAAGAGCCC AGACTCAGCC AGCATCCGTC TGTAGCACCA CATCCTCCAC CCCAGCAGCC 
GTCCCTGGGC AGCAGAGTAA GCGGGGTGCC ATGGGCTGGC AGAAGGCTGG GCACCCGCCG 
TCCGATGGCC AACAGAGAGC AACACAACAG GGCACTCTCA GGACCTTGCT TGGCGATGGC 
GTTGGCAGAC AGCAGCCGCG GACCAAACAG GTGTGCAATG TCGAGGTGGG TCTTCAGACC 
CAGGACAGGC CCCCTGCTAT GGGAGCCAAG CACAGCGGCA ATGGCCACTG CGTGCAGAAC 
TTTCCTCCCT CCCTGGCTTC GAAACCCAGC CTTGTCCAGC AGAAGCCTAC TGCCTATGTG 
CGGCCCATGG ATGGCCAAGA TCAGGCGCCC GATGAGTCTC CGAAGCTAAA GTCGTCTGCA 
GAGACTGGCG TGCACTGCTT GTACAGGGGT GTCCCGGCCA CCAAGTCAGA GTCAGCCAGA 
GCCAAGGCCA AGCTCTCCAA GTTCAGCATC CCCAAACAAG GAGAGGAGAG TCGATCTGGA 
GAAACCAACA GCTGTGTTGA AGAAATCATT CGGGAGATGA CCTGGCCTCC AGCACTTGCT 
GCTATTCAAC CACCTGGCAA AGTGGAACCA AGCAAATTTC CATTTCCAAA TAAGGACTCT 
CAGCTTGCCT CCTCTGGACA CAATAATCCA AAGAAAGGTG ATGCCGAGCC AGAGAGTCCA 
GACAATGGCA CATCGAACAC ATCCTCTCTG GGATGGTTAT CCCCATTTAC AGATAAGGGA 
AATGATGCAC TGAGAGGAAT GCTAGAAGAT GACCTTAAGC TAAGCAGCGA TGAAGAGGAG 
AATGAACAGG CAGCCCAGAG AACGGCCCTC CATGCTCTGT CTGACAGTGC CGGACTCCAG 
CCGGCCGACT GCAGGGCCTC CGCACCCTCG AGCAAGGGCA GCAGCAGCGG CAGCAGCAGC 
AGTGGGAGCA GCTCCTCCAG CGACTCGGAG AGCAGCTCGG GGTCCGACTC GGAGACAGAG 
AGCAGTTCCA GCGAGAGCGA GGGCAGCAAG CCTCCCCACT ACTCCAGTCC CGAGGCGGAA 
CCAGCGTCCT CTAACAAGTG GCAGCTGGAT AAATGGCTAA ACAAAGTGAA TCACCACAAG 
CCCCCTGTCC TGATCCAAAA TGAAAGCCAC CACGGGCCGG AGAGCAGTCA GTACTACACC 
CCAGTGAAGG AGGACACGCA GGACTGCGGG AAGCTCCCCG ACGTCTGCCC GCCCGGCCTG 
AGAGACAAGG ACATCAAGGG CGCCTGCAAG GAGGAGCAGA GGCCCAGGAC CGCGAACAAG 
GCCCCCGGGA GCAAAGGGGT GAAGCAGAAG TCCCCGCCCG CGGCCGTGGC CGTGGCCGTG 
ACCGCCGCCG CCGCCCCGCC GCCCGCGGTG CCTGGTGCGC CCGCCGAGAG CGCGCCCGCA 
CCTGCCCGGA GGTCCGCGGG CAAGAAGCCC ACCCGGCGCA CCGAGAGGAC CTCGGCCGGC 
GACAGCGGCC ACCGGCCCGA GGAGCCCGTA GCTGCCGAGG CGCTGGGGGC GAGCACGGGG 
GTTCCCCCGG AGCCCCCCAA GCCCCGGCCT TGCGGCAACA GCAGGACCAA CCACCGCAAG 
GAGCTGCGCT CGGCCGTGAC CTGCGAGAAG CGCCGCACTC GGGGGCTGAG CCGGATCGTC 
CCCAAGTCCA AGGAGTTCAT CGAGACGGAG TCGTCCTCTT CCTCCTCCTC CTCGGACTCG 
GACCTGGAGT CGGAGCAGGA GGACTACCCT CTGTCCAAGG CTGTGGCCGC CGCCGCCGCC 
GCTGCCGCTG CCTCCGGGAA CGACCCAAGG CTGAAGGAGG CTCCCAGCAG CATCAGCGGC 
GGCGGCCCCC GCGCTCCCGT GGGCTCCATC AACGCCAGGA CCACCAGCGA CATTGCCAAG 
GAGCTGGAGG AGCAGTTCTA CACACTGGTC CCCTTCGGCC GCAACGAGCT TCTCTCCCCG 
TTGAAGGACA GTGACGAGGT CCGGTCACTC TGGGTCAAAA TTGACTTGAC ACTTCTGTCC 
AGGATCCCGG AACACCTGCC CCAGGAGCCG GGGGTCTTGA GTGCTCCGGC AGCCAAGGAC 
TCTGAGAGCG CGCCCCCGAG CCACGCATCC GAGGCGCCGG TGGAAAAGAC CTTGCCAAAA 
TCCAAGAGGA AACGCAAGTG TGACAACGAA GACGATTACA GGGAGATCAA GAAGGCCCCG 
GGAGAGAGGG AGAGCGCCTC ACGACTGAGC GCCTCTGCCA ACAGTACTTT GTCCGCAAAT 
CATTGCAGCA TGAACATCAA CAGCTTGGCA ATACCAATAA ATAAAAATGA AAAAGTGCTC 
CGGTCGCCCA TCTCACCCCT CTCCGACGCA TCTAAACACA AATTCTCCGG CGAGGACTTG 
ACTTCCTCCA GCAGATCCAA CAGCAACGGT CTGTTCACCT CCACCTCCTC CAGCAAAAAG 
CATAAGGCAG AGAGCCAGCT GCAGAGCCAC GCCGCGGACC TCACGAAAGC GGCTCACAAC 
AATTCCGAAA ACATTCTCCA CAAGTCACGG CCGCAGACAG AGCCCTGGTC TCCAGGCTCC 
AACGGCCACC GGGACTGCAA GAGGCAGAAA CTCATCTTCG ACGATATGCC ACGCAGTGCA 
GATTATTTTA TGCAAGAAGC TAAGCGAATG AAGCATAAAG CAGATGCGAT GGTGGAAAAG 
TTTGGAAAGG CTCTGAACTA TGCAGAAGCA GCCTTGTCAT TCATTGAGTG TGGAAATGCC 
ATGGAACAGG GCCCCATGGA GTCCAAATCT CCTTACACCA TGTATTCAGA GACAGTTGAG 
CTCATCAGGT ATGCCATGAG ACTAAAAACC CACTCAGGTC CCAATGCCAC ACCAGAGGAT 
AAACAGCTGG CTGCATTATG TTACCGATGC CTGGCTCTCC TGTACTGGAG GATGTTTCGA 
CTCAAAAGGG ATCACGCTGT AAAGTATTCA AAAGCACTTA TCGACTATTT CAAGAATTCA 
TCTAAAGCTG CCCAAGCCCC ATCTCCATGG GGGGCCAGTG GAAAGAGCAC TGGGACCCCA 
TCCCCCATGT CTCCCAATCC GTCCCCCACC GGCTCCGTGG GCTCTCAGGG GAGCCTGTCC 
AACAGCAACG CCCTGTCCCC ATCCACCATC GTCAGCATCC CGCAGCGCAT CCACCAGATG 
GCGGCCAATC ACGTGAGCAT CACCAACAGC ATTCTCCACA GCTACGACTA CTGGGAGATG 
GCAGACAACC TGGCCAAGGA AAACAGAGAA TTCTTCCACG ACCTGGACCT GCTCATGGGG 
CCGGTCACCC TGCACAGCAG CATGGAGCAC CTGGTCCAGT ACTCCCAGCA GGGCCTGCAC 
TGGCTGCGAA ACAGCGCCCA CCTGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_015465297.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320783.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

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CloneID	OBa58185
Clone ID Related Accession (Same CDS sequence)	XM_015465297.1 , XM_010809810.2
Accession Version	XM_015465297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGTGTCTAC 
GAACCGGATA GAAACGTGTT ACGGAGGAAA GAACGAGAAA GAAGAAACCA GGAAGCTCAG 
CAGGACGATG GCGCGTTTAA TTCGAGTTAC TCCCTCTTTA GTGAACCCTA CAAGACTAAC 
AAGGGGGATG AGCTCTCCAA CCGGCTCCAG AACACTTTAG GCAATTACGA TGAAATGAAA 
GACTTTCTCA CGGATCGATC AAACCAGAGT CATCTTGTGG GTGTTCCCAA ACCTGGGGTT 
CCTCAGGCTC CCGTGACCAA GATTGATGAA CATTTTGTGG CAGATTCAAG AGCCCAGACT 
CAGCCAGCAT CCGTCTGTAG CACCACATCC TCCACCCCAG CAGCCGTCCC TGGGCAGCAG 
AGTAAGCGGG GTGCCATGGG CTGGCAGAAG GCTGGGCACC CGCCGTCCGA TGGCCAACAG 
AGAGCAACAC AACAGGGCAC TCTCAGGACC TTGCTTGGCG ATGGCGTTGG CAGACAGCAG 
CCGCGGACCA AACAGGTGTG CAATGTCGAG GTGGGTCTTC AGACCCAGGA CAGGCCCCCT 
GCTATGGGAG CCAAGCACAG CGGCAATGGC CACTGCGTGC AGAACTTTCC TCCCTCCCTG 
GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG CCTACTGCCT ATGTGCGGCC CATGGATGGC 
CAAGATCAGG CGCCCGATGA GTCTCCGAAG CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC 
TGCTTGTACA GGGGTGTCCC GGCCACCAAG TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC 
TCCAAGTTCA GCATCCCCAA ACAAGGAGAG GAGAGTCGAT CTGGAGAAAC CAACAGCTGT 
GTTGAAGAAA TCATTCGGGA GATGACCTGG CCTCCAGCAC TTGCTGCTAT TCAACCACCT 
GGCAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGCCTCCTCT 
GGACACAATA ATCCAAAGAA AGGTGATGCC GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AACACATCCT CTCTGGGATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACTGAGA 
GGAATGCTAG AAGATGACCT TAAGCTAAGC AGCGATGAAG AGGAGAATGA ACAGGCAGCC 
CAGAGAACGG CCCTCCATGC TCTGTCTGAC AGTGCCGGAC TCCAGCCGGC CGACTGCAGG 
GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC 
TCCAGCGACT CGGAGAGCAG CTCGGGGTCC GACTCGGAGA CAGAGAGCAG TTCCAGCGAG 
AGCGAGGGCA GCAAGCCTCC CCACTACTCC AGTCCCGAGG CGGAACCAGC GTCCTCTAAC 
AAGTGGCAGC TGGATAAATG GCTAAACAAA GTGAATCACC ACAAGCCCCC TGTCCTGATC 
CAAAATGAAA GCCACCACGG GCCGGAGAGC AGTCAGTACT ACACCCCAGT GAAGGAGGAC 
ACGCAGGACT GCGGGAAGCT CCCCGACGTC TGCCCGCCCG GCCTGAGAGA CAAGGACATC 
AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC AGGACCGCGA ACAAGGCCCC CGGGAGCAAA 
GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC GTGGCCGTGG CCGTGACCGC CGCCGCCGCC 
CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC GAGAGCGCGC CCGCACCTGC CCGGAGGTCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAG AGGACCTCGG CCGGCGACAG CGGCCACCGG 
CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC 
CCCAAGCCCC GGCCTTGCGG CAACAGCAGG ACCAACCACC GCAAGGAGCT GCGCTCGGCC 
GTGACCTGCG AGAAGCGCCG CACTCGGGGG CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG 
TTCATCGAGA CGGAGTCGTC CTCTTCCTCC TCCTCCTCGG ACTCGGACCT GGAGTCGGAG 
CAGGAGGACT ACCCTCTGTC CAAGGCTGTG GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC 
GGGAACGACC CAAGGCTGAA GGAGGCTCCC AGCAGCATCA GCGGCGGCGG CCCCCGCGCT 
CCCGTGGGCT CCATCAACGC CAGGACCACC AGCGACATTG CCAAGGAGCT GGAGGAGCAG 
TTCTACACAC TGGTCCCCTT CGGCCGCAAC GAGCTTCTCT CCCCGTTGAA GGACAGTGAC 
GAGGTCCGGT CACTCTGGGT CAAAATTGAC TTGACACTTC TGTCCAGGAT CCCGGAACAC 
CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT CCGGCAGCCA AGGACTCTGA GAGCGCGCCC 
CCGAGCCACG CATCCGAGGC GCCGGTGGAA AAGACCTTGC CAAAATCCAA GAGGAAACGC 
AAGTGTGACA ACGAAGACGA TTACAGGGAG ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC 
GCCTCACGAC TGAGCGCCTC TGCCAACAGT ACTTTGTCCG CAAATCATTG CAGCATGAAC 
ATCAACAGCT TGGCAATACC AATAAATAAA AATGAAAAAG TGCTCCGGTC GCCCATCTCA 
CCCCTCTCCG ACGCATCTAA ACACAAATTC TCCGGCGAGG ACTTGACTTC CTCCAGCAGA 
TCCAACAGCA ACGGTCTGTT CACCTCCACC TCCTCCAGCA AAAAGCATAA GGCAGAGAGC 
CAGCTGCAGA GCCACGCCGC GGACCTCACG AAAGCGGCTC ACAACAATTC CGAAAACATT 
CTCCACAAGT CACGGCCGCA GACAGAGCCC TGGTCTCCAG GCTCCAACGG CCACCGGGAC 
TGCAAGAGGC AGAAACTCAT CTTCGACGAT ATGCCACGCA GTGCAGATTA TTTTATGCAA 
GAAGCTAAGC GAATGAAGCA TAAAGCAGAT GCGATGGTGG AAAAGTTTGG AAAGGCTCTG 
AACTATGCAG AAGCAGCCTT GTCATTCATT GAGTGTGGAA ATGCCATGGA ACAGGGCCCC 
ATGGAGTCCA AATCTCCTTA CACCATGTAT TCAGAGACAG TTGAGCTCAT CAGGTATGCC 
ATGAGACTAA AAACCCACTC AGGTCCCAAT GCCACACCAG AGGATAAACA GCTGGCTGCA 
TTATGTTACC GATGCCTGGC TCTCCTGTAC TGGAGGATGT TTCGACTCAA AAGGGATCAC 
GCTGTAAAGT ATTCAAAAGC ACTTATCGAC TATTTCAAGA ATTCATCTAA AGCTGCCCAA 
GCCCCATCTC CATGGGGGGC CAGTGGAAAG AGCACTGGGA CCCCATCCCC CATGTCTCCC 
AATCCGTCCC CCACCGGCTC CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG 
TCCCCATCCA CCATCGTCAG CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG 
AGCATCACCA ACAGCATTCT CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC 
AAGGAAAACA GAGAATTCTT CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC 
AGCAGCATGG AGCACCTGGT CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC 
GCCCACCTGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320783.1
CDS	230..3964
Translation	MDSFDLALLQEWDLESLCVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_015465297.1
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465297.1

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGTGTCTAC 
GAACCGGATA GAAACGTGTT ACGGAGGAAA GAACGAGAAA GAAGAAACCA GGAAGCTCAG 
CAGGACGATG GCGCGTTTAA TTCGAGTTAC TCCCTCTTTA GTGAACCCTA CAAGACTAAC 
AAGGGGGATG AGCTCTCCAA CCGGCTCCAG AACACTTTAG GCAATTACGA TGAAATGAAA 
GACTTTCTCA CGGATCGATC AAACCAGAGT CATCTTGTGG GTGTTCCCAA ACCTGGGGTT 
CCTCAGGCTC CCGTGACCAA GATTGATGAA CATTTTGTGG CAGATTCAAG AGCCCAGACT 
CAGCCAGCAT CCGTCTGTAG CACCACATCC TCCACCCCAG CAGCCGTCCC TGGGCAGCAG 
AGTAAGCGGG GTGCCATGGG CTGGCAGAAG GCTGGGCACC CGCCGTCCGA TGGCCAACAG 
AGAGCAACAC AACAGGGCAC TCTCAGGACC TTGCTTGGCG ATGGCGTTGG CAGACAGCAG 
CCGCGGACCA AACAGGTGTG CAATGTCGAG GTGGGTCTTC AGACCCAGGA CAGGCCCCCT 
GCTATGGGAG CCAAGCACAG CGGCAATGGC CACTGCGTGC AGAACTTTCC TCCCTCCCTG 
GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG CCTACTGCCT ATGTGCGGCC CATGGATGGC 
CAAGATCAGG CGCCCGATGA GTCTCCGAAG CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC 
TGCTTGTACA GGGGTGTCCC GGCCACCAAG TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC 
TCCAAGTTCA GCATCCCCAA ACAAGGAGAG GAGAGTCGAT CTGGAGAAAC CAACAGCTGT 
GTTGAAGAAA TCATTCGGGA GATGACCTGG CCTCCAGCAC TTGCTGCTAT TCAACCACCT 
GGCAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGCCTCCTCT 
GGACACAATA ATCCAAAGAA AGGTGATGCC GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AACACATCCT CTCTGGGATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACTGAGA 
GGAATGCTAG AAGATGACCT TAAGCTAAGC AGCGATGAAG AGGAGAATGA ACAGGCAGCC 
CAGAGAACGG CCCTCCATGC TCTGTCTGAC AGTGCCGGAC TCCAGCCGGC CGACTGCAGG 
GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC 
TCCAGCGACT CGGAGAGCAG CTCGGGGTCC GACTCGGAGA CAGAGAGCAG TTCCAGCGAG 
AGCGAGGGCA GCAAGCCTCC CCACTACTCC AGTCCCGAGG CGGAACCAGC GTCCTCTAAC 
AAGTGGCAGC TGGATAAATG GCTAAACAAA GTGAATCACC ACAAGCCCCC TGTCCTGATC 
CAAAATGAAA GCCACCACGG GCCGGAGAGC AGTCAGTACT ACACCCCAGT GAAGGAGGAC 
ACGCAGGACT GCGGGAAGCT CCCCGACGTC TGCCCGCCCG GCCTGAGAGA CAAGGACATC 
AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC AGGACCGCGA ACAAGGCCCC CGGGAGCAAA 
GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC GTGGCCGTGG CCGTGACCGC CGCCGCCGCC 
CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC GAGAGCGCGC CCGCACCTGC CCGGAGGTCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAG AGGACCTCGG CCGGCGACAG CGGCCACCGG 
CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC 
CCCAAGCCCC GGCCTTGCGG CAACAGCAGG ACCAACCACC GCAAGGAGCT GCGCTCGGCC 
GTGACCTGCG AGAAGCGCCG CACTCGGGGG CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG 
TTCATCGAGA CGGAGTCGTC CTCTTCCTCC TCCTCCTCGG ACTCGGACCT GGAGTCGGAG 
CAGGAGGACT ACCCTCTGTC CAAGGCTGTG GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC 
GGGAACGACC CAAGGCTGAA GGAGGCTCCC AGCAGCATCA GCGGCGGCGG CCCCCGCGCT 
CCCGTGGGCT CCATCAACGC CAGGACCACC AGCGACATTG CCAAGGAGCT GGAGGAGCAG 
TTCTACACAC TGGTCCCCTT CGGCCGCAAC GAGCTTCTCT CCCCGTTGAA GGACAGTGAC 
GAGGTCCGGT CACTCTGGGT CAAAATTGAC TTGACACTTC TGTCCAGGAT CCCGGAACAC 
CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT CCGGCAGCCA AGGACTCTGA GAGCGCGCCC 
CCGAGCCACG CATCCGAGGC GCCGGTGGAA AAGACCTTGC CAAAATCCAA GAGGAAACGC 
AAGTGTGACA ACGAAGACGA TTACAGGGAG ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC 
GCCTCACGAC TGAGCGCCTC TGCCAACAGT ACTTTGTCCG CAAATCATTG CAGCATGAAC 
ATCAACAGCT TGGCAATACC AATAAATAAA AATGAAAAAG TGCTCCGGTC GCCCATCTCA 
CCCCTCTCCG ACGCATCTAA ACACAAATTC TCCGGCGAGG ACTTGACTTC CTCCAGCAGA 
TCCAACAGCA ACGGTCTGTT CACCTCCACC TCCTCCAGCA AAAAGCATAA GGCAGAGAGC 
CAGCTGCAGA GCCACGCCGC GGACCTCACG AAAGCGGCTC ACAACAATTC CGAAAACATT 
CTCCACAAGT CACGGCCGCA GACAGAGCCC TGGTCTCCAG GCTCCAACGG CCACCGGGAC 
TGCAAGAGGC AGAAACTCAT CTTCGACGAT ATGCCACGCA GTGCAGATTA TTTTATGCAA 
GAAGCTAAGC GAATGAAGCA TAAAGCAGAT GCGATGGTGG AAAAGTTTGG AAAGGCTCTG 
AACTATGCAG AAGCAGCCTT GTCATTCATT GAGTGTGGAA ATGCCATGGA ACAGGGCCCC 
ATGGAGTCCA AATCTCCTTA CACCATGTAT TCAGAGACAG TTGAGCTCAT CAGGTATGCC 
ATGAGACTAA AAACCCACTC AGGTCCCAAT GCCACACCAG AGGATAAACA GCTGGCTGCA 
TTATGTTACC GATGCCTGGC TCTCCTGTAC TGGAGGATGT TTCGACTCAA AAGGGATCAC 
GCTGTAAAGT ATTCAAAAGC ACTTATCGAC TATTTCAAGA ATTCATCTAA AGCTGCCCAA 
GCCCCATCTC CATGGGGGGC CAGTGGAAAG AGCACTGGGA CCCCATCCCC CATGTCTCCC 
AATCCGTCCC CCACCGGCTC CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG 
TCCCCATCCA CCATCGTCAG CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG 
AGCATCACCA ACAGCATTCT CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC 
AAGGAAAACA GAGAATTCTT CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC 
AGCAGCATGG AGCACCTGGT CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC 
GCCCACCTGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF3 ( XM_010809810.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808112.1 Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa58185
Clone ID Related Accession (Same CDS sequence)	XM_015465297.1 , XM_010809810.2
Accession Version	XM_010809810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	AF4/FMR2 family member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809810.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGTGTCTAC 
GAACCGGATA GAAACGTGTT ACGGAGGAAA GAACGAGAAA GAAGAAACCA GGAAGCTCAG 
CAGGACGATG GCGCGTTTAA TTCGAGTTAC TCCCTCTTTA GTGAACCCTA CAAGACTAAC 
AAGGGGGATG AGCTCTCCAA CCGGCTCCAG AACACTTTAG GCAATTACGA TGAAATGAAA 
GACTTTCTCA CGGATCGATC AAACCAGAGT CATCTTGTGG GTGTTCCCAA ACCTGGGGTT 
CCTCAGGCTC CCGTGACCAA GATTGATGAA CATTTTGTGG CAGATTCAAG AGCCCAGACT 
CAGCCAGCAT CCGTCTGTAG CACCACATCC TCCACCCCAG CAGCCGTCCC TGGGCAGCAG 
AGTAAGCGGG GTGCCATGGG CTGGCAGAAG GCTGGGCACC CGCCGTCCGA TGGCCAACAG 
AGAGCAACAC AACAGGGCAC TCTCAGGACC TTGCTTGGCG ATGGCGTTGG CAGACAGCAG 
CCGCGGACCA AACAGGTGTG CAATGTCGAG GTGGGTCTTC AGACCCAGGA CAGGCCCCCT 
GCTATGGGAG CCAAGCACAG CGGCAATGGC CACTGCGTGC AGAACTTTCC TCCCTCCCTG 
GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG CCTACTGCCT ATGTGCGGCC CATGGATGGC 
CAAGATCAGG CGCCCGATGA GTCTCCGAAG CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC 
TGCTTGTACA GGGGTGTCCC GGCCACCAAG TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC 
TCCAAGTTCA GCATCCCCAA ACAAGGAGAG GAGAGTCGAT CTGGAGAAAC CAACAGCTGT 
GTTGAAGAAA TCATTCGGGA GATGACCTGG CCTCCAGCAC TTGCTGCTAT TCAACCACCT 
GGCAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGCCTCCTCT 
GGACACAATA ATCCAAAGAA AGGTGATGCC GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AACACATCCT CTCTGGGATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACTGAGA 
GGAATGCTAG AAGATGACCT TAAGCTAAGC AGCGATGAAG AGGAGAATGA ACAGGCAGCC 
CAGAGAACGG CCCTCCATGC TCTGTCTGAC AGTGCCGGAC TCCAGCCGGC CGACTGCAGG 
GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC 
TCCAGCGACT CGGAGAGCAG CTCGGGGTCC GACTCGGAGA CAGAGAGCAG TTCCAGCGAG 
AGCGAGGGCA GCAAGCCTCC CCACTACTCC AGTCCCGAGG CGGAACCAGC GTCCTCTAAC 
AAGTGGCAGC TGGATAAATG GCTAAACAAA GTGAATCACC ACAAGCCCCC TGTCCTGATC 
CAAAATGAAA GCCACCACGG GCCGGAGAGC AGTCAGTACT ACACCCCAGT GAAGGAGGAC 
ACGCAGGACT GCGGGAAGCT CCCCGACGTC TGCCCGCCCG GCCTGAGAGA CAAGGACATC 
AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC AGGACCGCGA ACAAGGCCCC CGGGAGCAAA 
GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC GTGGCCGTGG CCGTGACCGC CGCCGCCGCC 
CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC GAGAGCGCGC CCGCACCTGC CCGGAGGTCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAG AGGACCTCGG CCGGCGACAG CGGCCACCGG 
CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC 
CCCAAGCCCC GGCCTTGCGG CAACAGCAGG ACCAACCACC GCAAGGAGCT GCGCTCGGCC 
GTGACCTGCG AGAAGCGCCG CACTCGGGGG CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG 
TTCATCGAGA CGGAGTCGTC CTCTTCCTCC TCCTCCTCGG ACTCGGACCT GGAGTCGGAG 
CAGGAGGACT ACCCTCTGTC CAAGGCTGTG GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC 
GGGAACGACC CAAGGCTGAA GGAGGCTCCC AGCAGCATCA GCGGCGGCGG CCCCCGCGCT 
CCCGTGGGCT CCATCAACGC CAGGACCACC AGCGACATTG CCAAGGAGCT GGAGGAGCAG 
TTCTACACAC TGGTCCCCTT CGGCCGCAAC GAGCTTCTCT CCCCGTTGAA GGACAGTGAC 
GAGGTCCGGT CACTCTGGGT CAAAATTGAC TTGACACTTC TGTCCAGGAT CCCGGAACAC 
CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT CCGGCAGCCA AGGACTCTGA GAGCGCGCCC 
CCGAGCCACG CATCCGAGGC GCCGGTGGAA AAGACCTTGC CAAAATCCAA GAGGAAACGC 
AAGTGTGACA ACGAAGACGA TTACAGGGAG ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC 
GCCTCACGAC TGAGCGCCTC TGCCAACAGT ACTTTGTCCG CAAATCATTG CAGCATGAAC 
ATCAACAGCT TGGCAATACC AATAAATAAA AATGAAAAAG TGCTCCGGTC GCCCATCTCA 
CCCCTCTCCG ACGCATCTAA ACACAAATTC TCCGGCGAGG ACTTGACTTC CTCCAGCAGA 
TCCAACAGCA ACGGTCTGTT CACCTCCACC TCCTCCAGCA AAAAGCATAA GGCAGAGAGC 
CAGCTGCAGA GCCACGCCGC GGACCTCACG AAAGCGGCTC ACAACAATTC CGAAAACATT 
CTCCACAAGT CACGGCCGCA GACAGAGCCC TGGTCTCCAG GCTCCAACGG CCACCGGGAC 
TGCAAGAGGC AGAAACTCAT CTTCGACGAT ATGCCACGCA GTGCAGATTA TTTTATGCAA 
GAAGCTAAGC GAATGAAGCA TAAAGCAGAT GCGATGGTGG AAAAGTTTGG AAAGGCTCTG 
AACTATGCAG AAGCAGCCTT GTCATTCATT GAGTGTGGAA ATGCCATGGA ACAGGGCCCC 
ATGGAGTCCA AATCTCCTTA CACCATGTAT TCAGAGACAG TTGAGCTCAT CAGGTATGCC 
ATGAGACTAA AAACCCACTC AGGTCCCAAT GCCACACCAG AGGATAAACA GCTGGCTGCA 
TTATGTTACC GATGCCTGGC TCTCCTGTAC TGGAGGATGT TTCGACTCAA AAGGGATCAC 
GCTGTAAAGT ATTCAAAAGC ACTTATCGAC TATTTCAAGA ATTCATCTAA AGCTGCCCAA 
GCCCCATCTC CATGGGGGGC CAGTGGAAAG AGCACTGGGA CCCCATCCCC CATGTCTCCC 
AATCCGTCCC CCACCGGCTC CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG 
TCCCCATCCA CCATCGTCAG CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG 
AGCATCACCA ACAGCATTCT CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC 
AAGGAAAACA GAGAATTCTT CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC 
AGCAGCATGG AGCACCTGGT CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC 
GCCCACCTGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808112.1
CDS	230..3964
Translation	MDSFDLALLQEWDLESLCVYEPDRNVLRRKERERRNQEAQQDDGAFNSSYSLFSEPYKTNKGDELSNRLQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQAPVTKIDEHFVADSRAQTQPASVCSTTSSTPAAVPGQQSKRGAMGWQKAGHPPSDGQQRATQQGTLRTLLGDGVGRQQPRTKQVCNVEVGLQTQDRPPAMGAKHSGNGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSAETGVHCLYRGVPATKSESARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWPPALAAIQPPGKVEPSKFPFPNKDSQLASSGHNNPKKGDAEPESPDNGTSNTSSLGWLSPFTDKGNDALRGMLEDDLKLSSDEEENEQAAQRTALHALSDSAGLQPADCRASAPSSKGSSSGSSSSGSSSSSDSESSSGSDSETESSSSESEGSKPPHYSSPEAEPASSNKWQLDKWLNKVNHHKPPVLIQNESHHGPESSQYYTPVKEDTQDCGKLPDVCPPGLRDKDIKGACKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVTAAAAPPPAVPGAPAESAPAPARRSAGKKPTRRTERTSAGDSGHRPEEPVAAEALGASTGVPPEPPKPRPCGNSRTNHRKELRSAVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEDYPLSKAVAAAAAAAAASGNDPRLKEAPSSISGGGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDLTLLSRIPEHLPQEPGVLSAPAAKDSESAPPSHASEAPVEKTLPKSKRKRKCDNEDDYREIKKAPGERESASRLSASANSTLSANHCSMNINSLAIPINKNEKVLRSPISPLSDASKHKFSGEDLTSSSRSNSNGLFTSTSSSKKHKAESQLQSHAADLTKAAHNNSENILHKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPTGSVGSQGSLSNSNALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFHDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLA

Target ORF information:

RefSeq Version	XM_010809810.2
Organism	Bos taurus(cattle)
Definition	Bos taurus AF4/FMR2 family member 3 (AFF3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809810.2

ORF Insert Sequence:

ATGGACAGCT TCGACTTAGC CCTGCTCCAG GAATGGGACC TCGAGTCGCT GTGTGTCTAC 
GAACCGGATA GAAACGTGTT ACGGAGGAAA GAACGAGAAA GAAGAAACCA GGAAGCTCAG 
CAGGACGATG GCGCGTTTAA TTCGAGTTAC TCCCTCTTTA GTGAACCCTA CAAGACTAAC 
AAGGGGGATG AGCTCTCCAA CCGGCTCCAG AACACTTTAG GCAATTACGA TGAAATGAAA 
GACTTTCTCA CGGATCGATC AAACCAGAGT CATCTTGTGG GTGTTCCCAA ACCTGGGGTT 
CCTCAGGCTC CCGTGACCAA GATTGATGAA CATTTTGTGG CAGATTCAAG AGCCCAGACT 
CAGCCAGCAT CCGTCTGTAG CACCACATCC TCCACCCCAG CAGCCGTCCC TGGGCAGCAG 
AGTAAGCGGG GTGCCATGGG CTGGCAGAAG GCTGGGCACC CGCCGTCCGA TGGCCAACAG 
AGAGCAACAC AACAGGGCAC TCTCAGGACC TTGCTTGGCG ATGGCGTTGG CAGACAGCAG 
CCGCGGACCA AACAGGTGTG CAATGTCGAG GTGGGTCTTC AGACCCAGGA CAGGCCCCCT 
GCTATGGGAG CCAAGCACAG CGGCAATGGC CACTGCGTGC AGAACTTTCC TCCCTCCCTG 
GCTTCGAAAC CCAGCCTTGT CCAGCAGAAG CCTACTGCCT ATGTGCGGCC CATGGATGGC 
CAAGATCAGG CGCCCGATGA GTCTCCGAAG CTAAAGTCGT CTGCAGAGAC TGGCGTGCAC 
TGCTTGTACA GGGGTGTCCC GGCCACCAAG TCAGAGTCAG CCAGAGCCAA GGCCAAGCTC 
TCCAAGTTCA GCATCCCCAA ACAAGGAGAG GAGAGTCGAT CTGGAGAAAC CAACAGCTGT 
GTTGAAGAAA TCATTCGGGA GATGACCTGG CCTCCAGCAC TTGCTGCTAT TCAACCACCT 
GGCAAAGTGG AACCAAGCAA ATTTCCATTT CCAAATAAGG ACTCTCAGCT TGCCTCCTCT 
GGACACAATA ATCCAAAGAA AGGTGATGCC GAGCCAGAGA GTCCAGACAA TGGCACATCG 
AACACATCCT CTCTGGGATG GTTATCCCCA TTTACAGATA AGGGAAATGA TGCACTGAGA 
GGAATGCTAG AAGATGACCT TAAGCTAAGC AGCGATGAAG AGGAGAATGA ACAGGCAGCC 
CAGAGAACGG CCCTCCATGC TCTGTCTGAC AGTGCCGGAC TCCAGCCGGC CGACTGCAGG 
GCCTCCGCAC CCTCGAGCAA GGGCAGCAGC AGCGGCAGCA GCAGCAGTGG GAGCAGCTCC 
TCCAGCGACT CGGAGAGCAG CTCGGGGTCC GACTCGGAGA CAGAGAGCAG TTCCAGCGAG 
AGCGAGGGCA GCAAGCCTCC CCACTACTCC AGTCCCGAGG CGGAACCAGC GTCCTCTAAC 
AAGTGGCAGC TGGATAAATG GCTAAACAAA GTGAATCACC ACAAGCCCCC TGTCCTGATC 
CAAAATGAAA GCCACCACGG GCCGGAGAGC AGTCAGTACT ACACCCCAGT GAAGGAGGAC 
ACGCAGGACT GCGGGAAGCT CCCCGACGTC TGCCCGCCCG GCCTGAGAGA CAAGGACATC 
AAGGGCGCCT GCAAGGAGGA GCAGAGGCCC AGGACCGCGA ACAAGGCCCC CGGGAGCAAA 
GGGGTGAAGC AGAAGTCCCC GCCCGCGGCC GTGGCCGTGG CCGTGACCGC CGCCGCCGCC 
CCGCCGCCCG CGGTGCCTGG TGCGCCCGCC GAGAGCGCGC CCGCACCTGC CCGGAGGTCC 
GCGGGCAAGA AGCCCACCCG GCGCACCGAG AGGACCTCGG CCGGCGACAG CGGCCACCGG 
CCCGAGGAGC CCGTAGCTGC CGAGGCGCTG GGGGCGAGCA CGGGGGTTCC CCCGGAGCCC 
CCCAAGCCCC GGCCTTGCGG CAACAGCAGG ACCAACCACC GCAAGGAGCT GCGCTCGGCC 
GTGACCTGCG AGAAGCGCCG CACTCGGGGG CTGAGCCGGA TCGTCCCCAA GTCCAAGGAG 
TTCATCGAGA CGGAGTCGTC CTCTTCCTCC TCCTCCTCGG ACTCGGACCT GGAGTCGGAG 
CAGGAGGACT ACCCTCTGTC CAAGGCTGTG GCCGCCGCCG CCGCCGCTGC CGCTGCCTCC 
GGGAACGACC CAAGGCTGAA GGAGGCTCCC AGCAGCATCA GCGGCGGCGG CCCCCGCGCT 
CCCGTGGGCT CCATCAACGC CAGGACCACC AGCGACATTG CCAAGGAGCT GGAGGAGCAG 
TTCTACACAC TGGTCCCCTT CGGCCGCAAC GAGCTTCTCT CCCCGTTGAA GGACAGTGAC 
GAGGTCCGGT CACTCTGGGT CAAAATTGAC TTGACACTTC TGTCCAGGAT CCCGGAACAC 
CTGCCCCAGG AGCCGGGGGT CTTGAGTGCT CCGGCAGCCA AGGACTCTGA GAGCGCGCCC 
CCGAGCCACG CATCCGAGGC GCCGGTGGAA AAGACCTTGC CAAAATCCAA GAGGAAACGC 
AAGTGTGACA ACGAAGACGA TTACAGGGAG ATCAAGAAGG CCCCGGGAGA GAGGGAGAGC 
GCCTCACGAC TGAGCGCCTC TGCCAACAGT ACTTTGTCCG CAAATCATTG CAGCATGAAC 
ATCAACAGCT TGGCAATACC AATAAATAAA AATGAAAAAG TGCTCCGGTC GCCCATCTCA 
CCCCTCTCCG ACGCATCTAA ACACAAATTC TCCGGCGAGG ACTTGACTTC CTCCAGCAGA 
TCCAACAGCA ACGGTCTGTT CACCTCCACC TCCTCCAGCA AAAAGCATAA GGCAGAGAGC 
CAGCTGCAGA GCCACGCCGC GGACCTCACG AAAGCGGCTC ACAACAATTC CGAAAACATT 
CTCCACAAGT CACGGCCGCA GACAGAGCCC TGGTCTCCAG GCTCCAACGG CCACCGGGAC 
TGCAAGAGGC AGAAACTCAT CTTCGACGAT ATGCCACGCA GTGCAGATTA TTTTATGCAA 
GAAGCTAAGC GAATGAAGCA TAAAGCAGAT GCGATGGTGG AAAAGTTTGG AAAGGCTCTG 
AACTATGCAG AAGCAGCCTT GTCATTCATT GAGTGTGGAA ATGCCATGGA ACAGGGCCCC 
ATGGAGTCCA AATCTCCTTA CACCATGTAT TCAGAGACAG TTGAGCTCAT CAGGTATGCC 
ATGAGACTAA AAACCCACTC AGGTCCCAAT GCCACACCAG AGGATAAACA GCTGGCTGCA 
TTATGTTACC GATGCCTGGC TCTCCTGTAC TGGAGGATGT TTCGACTCAA AAGGGATCAC 
GCTGTAAAGT ATTCAAAAGC ACTTATCGAC TATTTCAAGA ATTCATCTAA AGCTGCCCAA 
GCCCCATCTC CATGGGGGGC CAGTGGAAAG AGCACTGGGA CCCCATCCCC CATGTCTCCC 
AATCCGTCCC CCACCGGCTC CGTGGGCTCT CAGGGGAGCC TGTCCAACAG CAACGCCCTG 
TCCCCATCCA CCATCGTCAG CATCCCGCAG CGCATCCACC AGATGGCGGC CAATCACGTG 
AGCATCACCA ACAGCATTCT CCACAGCTAC GACTACTGGG AGATGGCAGA CAACCTGGCC 
AAGGAAAACA GAGAATTCTT CCACGACCTG GACCTGCTCA TGGGGCCGGT CACCCTGCAC 
AGCAGCATGG AGCACCTGGT CCAGTACTCC CAGCAGGGCC TGCACTGGCT GCGAAACAGC 
GCCCACCTGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFF3
Entrez Gene ID	787488
Full Name	AF4/FMR2 family member 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	AFF3	NP_001020279.1
	LOC714408	XP_002808094.1
Canis lupus familiaris (dog)	AFF3	XP_005626071.1
Mus musculus (house mouse)	Aff3	NP_034808.2
Xenopus tropicalis (tropical clawed frog)	aff3	XP_002937914.2
Bos taurus (cattle)	AFF3	XP_002691193.1
Pan troglodytes (chimpanzee)	AFF3	XP_001160964.2
Rattus norvegicus (Norway rat)	Aff3	NP_001178816.1
Gallus gallus (chicken)	AFF3	XP_004938558.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFF3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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