CEP250 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014107038.2	XP_013962513.1	centrosome-associated protein CEP250 isoform X1
XM_022409435.1	XP_022265143.1	centrosome-associated protein CEP250 isoform X1
XM_005634976.3	XP_005635033.1	centrosome-associated protein CEP250 isoform X8
XM_014107038.1	XP_013962513.1	centrosome-associated protein CEP250 isoform X1
XM_005634972.2	XP_005635029.1	centrosome-associated protein CEP250 isoform X3
XM_014107041.1	XP_013962516.1	centrosome-associated protein CEP250 isoform X5
XM_005634976.2	XP_005635033.1	centrosome-associated protein CEP250 isoform X6
XM_014107037.1	XP_013962512.1	centrosome-associated protein CEP250 isoform X1
XM_014107039.2	XP_013962514.1	centrosome-associated protein CEP250 isoform X2
XM_014107040.2	XP_013962515.1	centrosome-associated protein CEP250 isoform X4
XM_022409439.1	XP_022265147.1	centrosome-associated protein CEP250 isoform X10
XM_022409440.1	XP_022265148.1	centrosome-associated protein CEP250 isoform X11
XM_014107037.2	XP_013962512.1	centrosome-associated protein CEP250 isoform X1
XM_022409436.1	XP_022265144.1	centrosome-associated protein CEP250 isoform X6
XM_022409438.1	XP_022265146.1	centrosome-associated protein CEP250 isoform X9
XM_014107040.1	XP_013962515.1	centrosome-associated protein CEP250 isoform X4
XM_014107039.1	XP_013962514.1	centrosome-associated protein CEP250 isoform X2
XM_005634972.3	XP_005635029.1	centrosome-associated protein CEP250 isoform X3
XM_014107041.2	XP_013962516.1	centrosome-associated protein CEP250 isoform X5
XM_022409437.1	XP_022265145.1	centrosome-associated protein CEP250 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296945	XM_022409436.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe296946	XM_022409437.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe296947	XM_022409438.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe296948	XM_022409439.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe296949	XM_022409440.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe42760	XM_014107037.2 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42760	XM_022409435.1 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42760	XM_014107038.2 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42761	XM_014107039.2 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42762	XM_014107040.2 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42763	XM_014107041.2 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe24790	XM_005634972.3 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe24793	XM_005634976.3 Latest version!	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe42760	XM_014107038.1	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe24793	XM_005634976.2	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe42760	XM_014107037.1	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42762	XM_014107040.1	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe42761	XM_014107039.1	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OCe24790	XM_005634972.2	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe42763	XM_014107041.1	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

CEP250 ( XM_022409436.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265144.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe296945
Clone ID Related Accession (Same CDS sequence)	XM_022409436.1
Accession Version	XM_022409436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG GTCCGTTTGG AGGAGGAACA 
ACAGAGTGCA GGTGTGAGAG TCTAGCAGAG GTGAACACCC AGCTTCGGCT GCACATGGAA 
AAAGCTGACA TGGTGAATAA AGCACTTCGG GAAGATGTGG AAAACCTGAC AGTGGACTGG 
AGCCGAGCCC GGGATGAGCT CAGGAGGAAG GAGAGCCAGT GGCAGATGGA GCAGGAGTTC 
TTCAAGGGCT ACCTGAAAGG GGAGCACGGT CGCCTTCTTG GTCTGTGGCG GGAGGTGGTG 
ACCTTCCGAC GCCACTTCCT GGAGATGAAG TCAGCTACTG ACAGAGATCT GACAGAGCTC 
AAGGCTGAAC ATGTGAAGCT TTCAGGGTCC CTGTTGACAT GTTGTCTGCG CTTGACTGTG 
GGCACCCAGT CTCGAGAGTC GGATGGATCT GGGAGACGGG ATGGGAGTGA GCCAACCCAG 
CTGCTGCTAC TACTGACCAA GACCCAGGAG CTGGAGAAGG AAGCGCATGA AAGGAGCCAG 
GAGCTAATAC AGCTGAAGAG TCGGGGCGAT CTGGAGAAGG CTGAGCTGCA GGATCAGGTG 
ACGGAGCTCT CTGCTCTGCT GATCCAGGCT CGGAAGCAAA ATGAAGAGTA TGAGAAGATG 
TTAGGGGCCC TGAGAGAGAC AGTGGAGATC CTGGAGACAA ATCATGCAGA ATTAATGGAA 
CATGAGGTAT CTCTCAGTAA GAATGCCCAA GAGGAGAAGC TGTCTTTACA GCAGGTGATC 
AGGGATATAA CCCAGGTACT GGTCATGGTG GAAGAAGGGG ACAGTATGAC CCAAGGCTGT 
GGTCGAGACA GCTCCTTAGA ATTGGACCCT AGTGGCCTCT CATCCCAGTT TGATTCCCAG 
GACCCAGACA AGGCCCTTAC TCTGGTGCGT TCAGTGGTGA CTCAAAGACG CCAGGCTGTG 
CAGGACCTAA GGCAGCAGCT TTCGGCCTGT CAGGAAGCTA TGAGCTCTTT GCGGCAGCAG 
CATAATCAGT GGGAGGAGGA GGGTGAGGCC TTAAGACAGC GTCTGCAGAA GCTCACCGGG 
GAACGCGACA CTCTGGCAGG GCAGACCTCG GACCTACAGG GAGAGGTGGA GTCTCTCAGC 
AAGGAGCGAG AGCTCCTGCA GAAGACCAGG GAGGAGCTGC AGCAGCAGTT GGAGGTGCTG 
GAGCAGGAGG CATGGCGGCT GCGAAGGACA AACATGGAGC TTCAGTTGCA GGGGGATTCT 
GTTCAGGGTG AGAAGGAGGA GCAGCAGGAG GAGCTGCACC TGGCTGTCCG TGAAAGGGAG 
CGCCTTCAGG AGACACTAGC AAGTCTGGAA GCCAAACAGT CAGAATCACT CAGTGAATTG 
ATCATTCTTC GGGAAGCCCT GGAGTCTTGT CACCTGGAAG GGGAGCTGCT GAGGCAAGAG 
CAAACAGAAG TGACTGCGGC GCTGGCCAGG GCAGAACAGT CAGTTGCAGA GCTGTCAAGT 
TCTGAAAACA GCCTGAAGGC CGAGGTTGCT GATCTTCGGG CTGCAACCAT CAAGCTCAGC 
GCCTTAAATG AGGCTTTGGC CTTGGATAAG GTTGGACTGA ACCAGCAGCT TCTCCAGTTA 
GAACAAGAGA ACCAATCTGT GTGCCACAGA ATGGAAGCAA CAGAGCAGGC AAGAAACGCT 
TTGCAGTTGG GCCTGGCAGA GGCCGAGAGG AGCAGGGAAG CCCTACAGGA AAAGAACACT 
CACCTGGAGG CACAGCTGCA GAAGGCAGAG GAGAGGGGGG CTGAGCTGCA GGCAGATCTC 
AGGGCCATCC AAGATGAGAA GGAAGAAATT CAAGAGAAAC TAAGTGAGAT TTCTTCCTCT 
CAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265144.1
CDS	155..7219
Translation	MGKHGGARPGTVAGPFGGGTTECRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_022409436.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409436.1

ORF Insert Sequence:

ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG GTCCGTTTGG AGGAGGAACA 
ACAGAGTGCA GGTGTGAGAG TCTAGCAGAG GTGAACACCC AGCTTCGGCT GCACATGGAA 
AAAGCTGACA TGGTGAATAA AGCACTTCGG GAAGATGTGG AAAACCTGAC AGTGGACTGG 
AGCCGAGCCC GGGATGAGCT CAGGAGGAAG GAGAGCCAGT GGCAGATGGA GCAGGAGTTC 
TTCAAGGGCT ACCTGAAAGG GGAGCACGGT CGCCTTCTTG GTCTGTGGCG GGAGGTGGTG 
ACCTTCCGAC GCCACTTCCT GGAGATGAAG TCAGCTACTG ACAGAGATCT GACAGAGCTC 
AAGGCTGAAC ATGTGAAGCT TTCAGGGTCC CTGTTGACAT GTTGTCTGCG CTTGACTGTG 
GGCACCCAGT CTCGAGAGTC GGATGGATCT GGGAGACGGG ATGGGAGTGA GCCAACCCAG 
CTGCTGCTAC TACTGACCAA GACCCAGGAG CTGGAGAAGG AAGCGCATGA AAGGAGCCAG 
GAGCTAATAC AGCTGAAGAG TCGGGGCGAT CTGGAGAAGG CTGAGCTGCA GGATCAGGTG 
ACGGAGCTCT CTGCTCTGCT GATCCAGGCT CGGAAGCAAA ATGAAGAGTA TGAGAAGATG 
TTAGGGGCCC TGAGAGAGAC AGTGGAGATC CTGGAGACAA ATCATGCAGA ATTAATGGAA 
CATGAGGTAT CTCTCAGTAA GAATGCCCAA GAGGAGAAGC TGTCTTTACA GCAGGTGATC 
AGGGATATAA CCCAGGTACT GGTCATGGTG GAAGAAGGGG ACAGTATGAC CCAAGGCTGT 
GGTCGAGACA GCTCCTTAGA ATTGGACCCT AGTGGCCTCT CATCCCAGTT TGATTCCCAG 
GACCCAGACA AGGCCCTTAC TCTGGTGCGT TCAGTGGTGA CTCAAAGACG CCAGGCTGTG 
CAGGACCTAA GGCAGCAGCT TTCGGCCTGT CAGGAAGCTA TGAGCTCTTT GCGGCAGCAG 
CATAATCAGT GGGAGGAGGA GGGTGAGGCC TTAAGACAGC GTCTGCAGAA GCTCACCGGG 
GAACGCGACA CTCTGGCAGG GCAGACCTCG GACCTACAGG GAGAGGTGGA GTCTCTCAGC 
AAGGAGCGAG AGCTCCTGCA GAAGACCAGG GAGGAGCTGC AGCAGCAGTT GGAGGTGCTG 
GAGCAGGAGG CATGGCGGCT GCGAAGGACA AACATGGAGC TTCAGTTGCA GGGGGATTCT 
GTTCAGGGTG AGAAGGAGGA GCAGCAGGAG GAGCTGCACC TGGCTGTCCG TGAAAGGGAG 
CGCCTTCAGG AGACACTAGC AAGTCTGGAA GCCAAACAGT CAGAATCACT CAGTGAATTG 
ATCATTCTTC GGGAAGCCCT GGAGTCTTGT CACCTGGAAG GGGAGCTGCT GAGGCAAGAG 
CAAACAGAAG TGACTGCGGC GCTGGCCAGG GCAGAACAGT CAGTTGCAGA GCTGTCAAGT 
TCTGAAAACA GCCTGAAGGC CGAGGTTGCT GATCTTCGGG CTGCAACCAT CAAGCTCAGC 
GCCTTAAATG AGGCTTTGGC CTTGGATAAG GTTGGACTGA ACCAGCAGCT TCTCCAGTTA 
GAACAAGAGA ACCAATCTGT GTGCCACAGA ATGGAAGCAA CAGAGCAGGC AAGAAACGCT 
TTGCAGTTGG GCCTGGCAGA GGCCGAGAGG AGCAGGGAAG CCCTACAGGA AAAGAACACT 
CACCTGGAGG CACAGCTGCA GAAGGCAGAG GAGAGGGGGG CTGAGCTGCA GGCAGATCTC 
AGGGCCATCC AAGATGAGAA GGAAGAAATT CAAGAGAAAC TAAGTGAGAT TTCTTCCTCT 
CAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_022409437.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265145.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

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CloneID	OCe296946
Clone ID Related Accession (Same CDS sequence)	XM_022409437.1
Accession Version	XM_022409437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGACCACTC AGATCCTGCA GGCCCAGCTC 
CGGGAGGCTC GGGGGGAGCT GGAGCAGGCA GCCCAGCAGA ACAGAGATGA CCTTGTTGCT 
GTCCAAGAAG AGTGCGGGGC CCTGCTGCAG GCGAAGATGG ACCTGCAGAA GCGGGTAGAA 
GAGTTGAAGT CTCAGCTGGT TTCCAGAGAT GACTCCCGGA GGCTGGTGGA GCAGGAGGTT 
CAGGAGAAGC TGAGGGAGGC CCAGGAGTAT AGCCGAATTC AGAAGGAGCT GGAGAGAGAG 
AAAGCCAGCC TGATTCAGTC GCTGATGGAA AAGGAGCAGA GACTCCTTGT TTTACAAGAA 
GCTGACTCTG TTCGACAACA GGAGCTGAGC TCCCTGCGCC AGGACATGCA GGAGTCCCAG 
GAAGGGCAGA AAGAGCTCAG CACCCAGGTG GAATTACTGA AGCAGGAGGT GAAGGAAAAG 
GAGGCTGACT TTCTGGCTCA GGAAGCACAA CTGCTGGAGG AGCTGGAGAC ATCTCAAGTA 
ACAGAGCAGC GGCTGCGAGC TTCTTTGCGG GCCCTGGAAG CCAAGGCAGC CCAAGTCCAG 
CTGCGACTGC GCAGCACAGA GAACCAGTTG GCAGCTCTGG TGGCAGAGCA GCAGCCGGGG 
CACCAGGCCC AGGCCCAGCT GGCCAGCCTC TGTTCTGTCC TGCAGCAGGC CTTGGGGTTT 
GCTTGTGAGA GCAGGCCTGA GCTGCGTGGC GGGGGAGACT CTGCTTCCCT CTGGGGCCCC 
GAGCCAGACC AGAATGGAAC TGGGATCCTC CTTAAGAGAG GGCCCCTCCT GACAGCTCTG 
TCAGCTGAGG CAGTGGCATC TGCCCTCCAG AAACTTCACC AAGACCTATG GAAGACTCAG 
CAGGCCCGGG ATGATCTGCG GGAACAGGCC CTGAAGCTGG AACAGCGTCT CACTGATACA 
GAGGCCGAGA AGAGTCAGGT CTGCACAGAA TTGCAGGATT TGCAGAGACA ACTCTCCCAG 
AACCAGGAAG AGAAATCCAA GTGGGAAGGA AAACAGATCT CCCTGGAATC TGAGCTGACT 
GAACTGCATG GAACTGTGGC ATCATTACAG AGTCGTCTAC AGCAAGCAGA GCTGCAGGGA 
CTAGAGGCCC AGAATGAGCG AAAGCTACTG CAGGCAGCCA AGGAGAGCCT GACAGCCCAG 
GTGGAACGTT TGCAGGCATC TGTGGCAGAA GCCAGGGCTC AGGCTGGTGC CACCAGGGCT 
CTGGAGGAGG ACTTGAGAAC TGCTCGCTCA GCCCTGAAAC TCAAGAGTGA GGAAGCAGAG 
ACCGAGCGTG AGCGGGCCCA GGCTCTGCAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAGGCTCTGC AGGAGAATTT AGCTATCCTG GTTCAGACTC TATCCGAAAG AGAAGGGGAG 
GTGGAGGCTT TGCGGGGAAG TATTCAGGAA CTGGAGAAAC AACGGGAGAT GCAAAAGGCT 
ACTTTGGAAG CTCTGTCTCT GGACCTGAAG AAGAAGAGCG AAGAGGTAGA TGTGCAACAA 
GAACAGATCC AGGAGCTGGA GAAGTGCAGG TCCCTTTTAG AAGATCTGCC TCTGGCCATG 
CAGGAACAAG AGCAGAGGCT GGTTGCACAG AGGGAGCAAA TCCAAGAGCT CGAGAAGGAT 
CGAGAGACCC AGAGGAACAT CTTGGAGCAT CAGCTTCTCG AACTTGAGAA GAAGGCCCAA 
ATGATAGAGT CCCAGAAAGG AGAGATTCAG GACCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCTC TGGAACGAGA GGAAAACCAT CACAAGATGG AGTGCCAACA GAAGGCAATC 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGAGTAGCTC TGACCCACCT CACACTGGAC 
CTGGAAGAAA AGAGCCAGGA GCTGCAGGCC CAGAGCAGTC AGATCGACAA GCTGGAGAGC 
CATAGCACCC TTCTGGCGAA AGAGCTCCAG GACAAGGACC AGGAGCTGAA GTCCCAGCAA 
GAACAGATCG AGGAGCTGCA GAGGCAGAAG GAGCGTCTGG CTCAGGACCT AGAGAGGAGG 
GACCAGGACG TGGTGCTCCA GAGGGAAAGG ATTCGGGTCC TAGAAGACCA GAGGACACTG 
CAGACCAAGA TCCTGCAGGA GGACCTGGGA CAGATCAAGC TGTCCTTGAG AGAGCGAGGC 
CGGGAGCTGG CTTCCCAGAG GCCACCGATG CCGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCCC AGCGCGGGAG CCTGGAGCAC CTGAAGCTGA TCCTGCGTGA CAAGGAGAGG 
GAGGTAGAAT GCCAGCAGGA ACGCATCCAG GAACTGAAGG AGTATAAGGA TCAGCTGGAG 
CAGCAGCTCC AGGGCCTACA CAGGAAGGCA GGGGAGACTG GCCTCCTCCT GACTCAGCGA 
GAGCAGGAGA TAGTGGTCCT GCAGCGGCAT CTGCAGGAAG CCGCAGAACA GGGGGAGCTG 
AAAGAGCGGT CACTTCAGGG TCACCTGGAA GAGGCCCAGA GAGCCCTGGC CCAGAGGGAC 
CAGGAGCTCG AGGCCCTGCA GCACCAACAG CAGCAGGCCC TGGGGCAGGA GGAGACTAGG 
AAGGAAGAGG CAAGCACCCT GCAGAGGGCT CTGGAGCAGG CCCACACGGC GCTGAAAGAG 
CGCCAGGGAG AGCTTGAGGA CCACAAGGAG CATGTGCGAA GGCTCCAGGA GGAGCTGGCC 
GTGGAGGGAC GGCGTGTGCA GGCCCTGGAG GAGGTGGTGG GTGACCTAAG GGCTGAGTCT 
CGGGAGCAGG AGGAGGCTTT GCTGGCCCTC CAGCAACAGG GTGCCGAGCG GGCACAGGAG 
CATGAGGCGG AGGTCAGGGG CCTGCGGGCC AGCCTCCTGC AGGCAGAGAC TGCACTCAAG 
GAACGGGACC TGGCGCTGGA GGCCCTGCGA GCCGATGGCC GGGCCTCCCA GCTTCGGGAG 
GAGACAGCCC GGGACTGGGC CCAAGCTCTG CAGGAGGCCC TGAGCAAGGC CCAGGCTGCC 
GTGCAGGAGA AAGAGCAGCG GCTGCTGGGT CAAGCAGAGT TGAGCCGCAG CCTGGAGACC 
AGCACCGCCA CTTTGCAGGC TGCCCTGGAC TCTTGCCAGG CACAAGCCCG GCAGCTGGAG 
GAGGCTCTGA GGAGGCGAGA GGACGAGATC CAGGACCGGG ACCTCCGGCA CCAGGAGGCC 
GTGCAGCAGC TCCAGCGGGC ACTTGCCCAG AGAGATGAAG AATTGAGCCA TCAGAAGAGA 
CAGGGGCAGC TGCTAGAGCA GTCTCTGGCC CGGAGGGCCC GAAAAGATGC CATCCAAGGG 
AAGCCAGGTC CGGAGCAGGA GAAAGAGGAA GAGATGAGAG GCCTTCGAGA AAGCCTAAGG 
GAGTTGCAGC TGACTCTAGC CCAAAAGGAA GAGGAGATCC TGGGGCTGAG GGAGGCCCAG 
CAAAGGAAGA ATCTGGAGGA CTCACTGCAC AGCCACACAG CGCCCCCAGA GCCCTCTACA 
GACTTTGCCA CCTTAGGGCC CAGGCTGCAG CAGGAGCTGG AGCGACTGCA GACAGCACTG 
AGGCTAACTG AGGCCAGGGA GATTGAGTGG AGGGAGAAGG CCCAGGACTT GGCACTGTCC 
CTGGCCCAGA GCAAGGCTAG CGTCAGCAGT TTGCAGGAGG CAGCCATGTT CCTACAGGCC 
TCTGTTCTGG AGCGGGACTT GGAACAGCAG AGGCTGCAGG ATGAGTTGGA GCTCACCAGA 
CAGGCTCTGG AGAAGGAGCA GCTCCTGAGC TCTAGTTCAA CCAGCAGAGC AGAACAGAGG 
CCCAGAGAAG AGGTGTCAGA AGTCGAGGCT GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG 
CTCTGGGGAC AAAGGCTGGA GTACCTCCAG CAAGCAGTGG CACAGCTGGA GATTGACCGG 
AGCAGGCTGC AGCACCACAA TGTCCAGCTG CGGGCCACCT TGGAGCAGGT GGAGCGAGAG 
CGCAGGAAGC TGAAGAGGGA GTCCATGCGC GTGTCACGGA CAGGCGGCCT GGAGGTTAAG 
GAAGCTGCGG CTTCGTCCCC CACACAGCAG GATGGAAGGG GAGGACAGAA GGGCTCCTCA 
GATGACAAGC AGATGGCTGA ACTGCAGAAA GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC 
TTGGAGCGGA AGCAGAGGCA GGACTACATT GCTCGCTCGG TGCAGACTAG CCGTGAGCTA 
GCGGGCCTGC ACCACAGCCT CTCCCACTCA CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC 
ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG GACGAGTCCC TGACTCAAAG TCTGACATCC 
CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265145.1
CDS	212..7228
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_022409437.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409437.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGACCACTC AGATCCTGCA GGCCCAGCTC 
CGGGAGGCTC GGGGGGAGCT GGAGCAGGCA GCCCAGCAGA ACAGAGATGA CCTTGTTGCT 
GTCCAAGAAG AGTGCGGGGC CCTGCTGCAG GCGAAGATGG ACCTGCAGAA GCGGGTAGAA 
GAGTTGAAGT CTCAGCTGGT TTCCAGAGAT GACTCCCGGA GGCTGGTGGA GCAGGAGGTT 
CAGGAGAAGC TGAGGGAGGC CCAGGAGTAT AGCCGAATTC AGAAGGAGCT GGAGAGAGAG 
AAAGCCAGCC TGATTCAGTC GCTGATGGAA AAGGAGCAGA GACTCCTTGT TTTACAAGAA 
GCTGACTCTG TTCGACAACA GGAGCTGAGC TCCCTGCGCC AGGACATGCA GGAGTCCCAG 
GAAGGGCAGA AAGAGCTCAG CACCCAGGTG GAATTACTGA AGCAGGAGGT GAAGGAAAAG 
GAGGCTGACT TTCTGGCTCA GGAAGCACAA CTGCTGGAGG AGCTGGAGAC ATCTCAAGTA 
ACAGAGCAGC GGCTGCGAGC TTCTTTGCGG GCCCTGGAAG CCAAGGCAGC CCAAGTCCAG 
CTGCGACTGC GCAGCACAGA GAACCAGTTG GCAGCTCTGG TGGCAGAGCA GCAGCCGGGG 
CACCAGGCCC AGGCCCAGCT GGCCAGCCTC TGTTCTGTCC TGCAGCAGGC CTTGGGGTTT 
GCTTGTGAGA GCAGGCCTGA GCTGCGTGGC GGGGGAGACT CTGCTTCCCT CTGGGGCCCC 
GAGCCAGACC AGAATGGAAC TGGGATCCTC CTTAAGAGAG GGCCCCTCCT GACAGCTCTG 
TCAGCTGAGG CAGTGGCATC TGCCCTCCAG AAACTTCACC AAGACCTATG GAAGACTCAG 
CAGGCCCGGG ATGATCTGCG GGAACAGGCC CTGAAGCTGG AACAGCGTCT CACTGATACA 
GAGGCCGAGA AGAGTCAGGT CTGCACAGAA TTGCAGGATT TGCAGAGACA ACTCTCCCAG 
AACCAGGAAG AGAAATCCAA GTGGGAAGGA AAACAGATCT CCCTGGAATC TGAGCTGACT 
GAACTGCATG GAACTGTGGC ATCATTACAG AGTCGTCTAC AGCAAGCAGA GCTGCAGGGA 
CTAGAGGCCC AGAATGAGCG AAAGCTACTG CAGGCAGCCA AGGAGAGCCT GACAGCCCAG 
GTGGAACGTT TGCAGGCATC TGTGGCAGAA GCCAGGGCTC AGGCTGGTGC CACCAGGGCT 
CTGGAGGAGG ACTTGAGAAC TGCTCGCTCA GCCCTGAAAC TCAAGAGTGA GGAAGCAGAG 
ACCGAGCGTG AGCGGGCCCA GGCTCTGCAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAGGCTCTGC AGGAGAATTT AGCTATCCTG GTTCAGACTC TATCCGAAAG AGAAGGGGAG 
GTGGAGGCTT TGCGGGGAAG TATTCAGGAA CTGGAGAAAC AACGGGAGAT GCAAAAGGCT 
ACTTTGGAAG CTCTGTCTCT GGACCTGAAG AAGAAGAGCG AAGAGGTAGA TGTGCAACAA 
GAACAGATCC AGGAGCTGGA GAAGTGCAGG TCCCTTTTAG AAGATCTGCC TCTGGCCATG 
CAGGAACAAG AGCAGAGGCT GGTTGCACAG AGGGAGCAAA TCCAAGAGCT CGAGAAGGAT 
CGAGAGACCC AGAGGAACAT CTTGGAGCAT CAGCTTCTCG AACTTGAGAA GAAGGCCCAA 
ATGATAGAGT CCCAGAAAGG AGAGATTCAG GACCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCTC TGGAACGAGA GGAAAACCAT CACAAGATGG AGTGCCAACA GAAGGCAATC 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGAGTAGCTC TGACCCACCT CACACTGGAC 
CTGGAAGAAA AGAGCCAGGA GCTGCAGGCC CAGAGCAGTC AGATCGACAA GCTGGAGAGC 
CATAGCACCC TTCTGGCGAA AGAGCTCCAG GACAAGGACC AGGAGCTGAA GTCCCAGCAA 
GAACAGATCG AGGAGCTGCA GAGGCAGAAG GAGCGTCTGG CTCAGGACCT AGAGAGGAGG 
GACCAGGACG TGGTGCTCCA GAGGGAAAGG ATTCGGGTCC TAGAAGACCA GAGGACACTG 
CAGACCAAGA TCCTGCAGGA GGACCTGGGA CAGATCAAGC TGTCCTTGAG AGAGCGAGGC 
CGGGAGCTGG CTTCCCAGAG GCCACCGATG CCGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCCC AGCGCGGGAG CCTGGAGCAC CTGAAGCTGA TCCTGCGTGA CAAGGAGAGG 
GAGGTAGAAT GCCAGCAGGA ACGCATCCAG GAACTGAAGG AGTATAAGGA TCAGCTGGAG 
CAGCAGCTCC AGGGCCTACA CAGGAAGGCA GGGGAGACTG GCCTCCTCCT GACTCAGCGA 
GAGCAGGAGA TAGTGGTCCT GCAGCGGCAT CTGCAGGAAG CCGCAGAACA GGGGGAGCTG 
AAAGAGCGGT CACTTCAGGG TCACCTGGAA GAGGCCCAGA GAGCCCTGGC CCAGAGGGAC 
CAGGAGCTCG AGGCCCTGCA GCACCAACAG CAGCAGGCCC TGGGGCAGGA GGAGACTAGG 
AAGGAAGAGG CAAGCACCCT GCAGAGGGCT CTGGAGCAGG CCCACACGGC GCTGAAAGAG 
CGCCAGGGAG AGCTTGAGGA CCACAAGGAG CATGTGCGAA GGCTCCAGGA GGAGCTGGCC 
GTGGAGGGAC GGCGTGTGCA GGCCCTGGAG GAGGTGGTGG GTGACCTAAG GGCTGAGTCT 
CGGGAGCAGG AGGAGGCTTT GCTGGCCCTC CAGCAACAGG GTGCCGAGCG GGCACAGGAG 
CATGAGGCGG AGGTCAGGGG CCTGCGGGCC AGCCTCCTGC AGGCAGAGAC TGCACTCAAG 
GAACGGGACC TGGCGCTGGA GGCCCTGCGA GCCGATGGCC GGGCCTCCCA GCTTCGGGAG 
GAGACAGCCC GGGACTGGGC CCAAGCTCTG CAGGAGGCCC TGAGCAAGGC CCAGGCTGCC 
GTGCAGGAGA AAGAGCAGCG GCTGCTGGGT CAAGCAGAGT TGAGCCGCAG CCTGGAGACC 
AGCACCGCCA CTTTGCAGGC TGCCCTGGAC TCTTGCCAGG CACAAGCCCG GCAGCTGGAG 
GAGGCTCTGA GGAGGCGAGA GGACGAGATC CAGGACCGGG ACCTCCGGCA CCAGGAGGCC 
GTGCAGCAGC TCCAGCGGGC ACTTGCCCAG AGAGATGAAG AATTGAGCCA TCAGAAGAGA 
CAGGGGCAGC TGCTAGAGCA GTCTCTGGCC CGGAGGGCCC GAAAAGATGC CATCCAAGGG 
AAGCCAGGTC CGGAGCAGGA GAAAGAGGAA GAGATGAGAG GCCTTCGAGA AAGCCTAAGG 
GAGTTGCAGC TGACTCTAGC CCAAAAGGAA GAGGAGATCC TGGGGCTGAG GGAGGCCCAG 
CAAAGGAAGA ATCTGGAGGA CTCACTGCAC AGCCACACAG CGCCCCCAGA GCCCTCTACA 
GACTTTGCCA CCTTAGGGCC CAGGCTGCAG CAGGAGCTGG AGCGACTGCA GACAGCACTG 
AGGCTAACTG AGGCCAGGGA GATTGAGTGG AGGGAGAAGG CCCAGGACTT GGCACTGTCC 
CTGGCCCAGA GCAAGGCTAG CGTCAGCAGT TTGCAGGAGG CAGCCATGTT CCTACAGGCC 
TCTGTTCTGG AGCGGGACTT GGAACAGCAG AGGCTGCAGG ATGAGTTGGA GCTCACCAGA 
CAGGCTCTGG AGAAGGAGCA GCTCCTGAGC TCTAGTTCAA CCAGCAGAGC AGAACAGAGG 
CCCAGAGAAG AGGTGTCAGA AGTCGAGGCT GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG 
CTCTGGGGAC AAAGGCTGGA GTACCTCCAG CAAGCAGTGG CACAGCTGGA GATTGACCGG 
AGCAGGCTGC AGCACCACAA TGTCCAGCTG CGGGCCACCT TGGAGCAGGT GGAGCGAGAG 
CGCAGGAAGC TGAAGAGGGA GTCCATGCGC GTGTCACGGA CAGGCGGCCT GGAGGTTAAG 
GAAGCTGCGG CTTCGTCCCC CACACAGCAG GATGGAAGGG GAGGACAGAA GGGCTCCTCA 
GATGACAAGC AGATGGCTGA ACTGCAGAAA GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC 
TTGGAGCGGA AGCAGAGGCA GGACTACATT GCTCGCTCGG TGCAGACTAG CCGTGAGCTA 
GCGGGCCTGC ACCACAGCCT CTCCCACTCA CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC 
ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG GACGAGTCCC TGACTCAAAG TCTGACATCC 
CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_022409438.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265146.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

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CloneID	OCe296947
Clone ID Related Accession (Same CDS sequence)	XM_022409438.1
Accession Version	XM_022409438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGAATAGTTC AGAAGAGGCT 
CCAGGAACAA TAAGACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265146.1
CDS	212..6892
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQNSSEEAPGTIRL

Target ORF information:

RefSeq Version	XM_022409438.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409438.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGAATAGTTC AGAAGAGGCT 
CCAGGAACAA TAAGACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_022409439.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265147.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X12, mRNA.

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CloneID	OCe296948
Clone ID Related Accession (Same CDS sequence)	XM_022409439.1
Accession Version	XM_022409439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGGGGTGAT TTGCTCCACA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265147.1
CDS	212..6877
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQGVICSTP

Target ORF information:

RefSeq Version	XM_022409439.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409439.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGGGGTGAT TTGCTCCACA 
CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_022409440.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265148.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X13, mRNA.

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CloneID	OCe296949
Clone ID Related Accession (Same CDS sequence)	XM_022409440.1
Accession Version	XM_022409440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CGGAGCTCTC TGCTCTGCTG ATCCAGGCTC GGAAGCAAAA TGAAGAGTAT 
GAGAAGATGT TAGGGGCCCT GAGAGAGACA GTGGAGATCC TGGAGACAAA TCATGCAGAA 
TTAATGGAAC ATGAGGTATC TCTCAGTAAG AATGCCCAAG AGGAGAAGCT GTCTTTACAG 
CAGGTGATCA GGGATATAAC CCAGGTACTG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265148.1
CDS	96..6569
Translation	MWVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_022409440.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409440.1

ORF Insert Sequence:

ATGTGGGTGA CGGAGCTCTC TGCTCTGCTG ATCCAGGCTC GGAAGCAAAA TGAAGAGTAT 
GAGAAGATGT TAGGGGCCCT GAGAGAGACA GTGGAGATCC TGGAGACAAA TCATGCAGAA 
TTAATGGAAC ATGAGGTATC TCTCAGTAAG AATGCCCAAG AGGAGAAGCT GTCTTTACAG 
CAGGTGATCA GGGATATAAC CCAGGTACTG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107038.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962513.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OCe42760
Clone ID Related Accession (Same CDS sequence)	XM_014107038.2 , XM_022409435.1 , XM_014107038.1 , XM_014107037.1 , XM_014107037.2
Accession Version	XM_014107038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962513.1
CDS	194..7513
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107038.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107038.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_022409435.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265143.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OCe42760
Clone ID Related Accession (Same CDS sequence)	XM_014107038.2 , XM_022409435.1 , XM_014107038.1 , XM_014107037.1 , XM_014107037.2
Accession Version	XM_022409435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265143.1
CDS	242..7561
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_022409435.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409435.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107038.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962513.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

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CloneID	OCe42760
Clone ID Related Accession (Same CDS sequence)	XM_014107038.2 , XM_022409435.1 , XM_014107038.1 , XM_014107037.1 , XM_014107037.2
Accession Version	XM_014107038.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962513.1
CDS	193..7512
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107038.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107038.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107037.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962512.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

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CloneID	OCe42760
Clone ID Related Accession (Same CDS sequence)	XM_014107038.2 , XM_022409435.1 , XM_014107038.1 , XM_014107037.1 , XM_014107037.2
Accession Version	XM_014107037.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962512.1
CDS	198..7517
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107037.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107037.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107037.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962512.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OCe42760
Clone ID Related Accession (Same CDS sequence)	XM_014107038.2 , XM_022409435.1 , XM_014107038.1 , XM_014107037.1 , XM_014107037.2
Accession Version	XM_014107037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962512.1
CDS	212..7531
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107037.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107037.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AGACAGGCTC TGGAGAAGGA GCAGCTCCTG AGCTCTAGTT CAACCAGCAG AGCAGAACAG 
AGGCCCAGAG AAGAGGTGTC AGAAGTCGAG GCTGAGCCTA GTCTTGGGCT GGAGGAGAGG 
CAGCTCTGGG GACAAAGGCT GGAGTACCTC CAGCAAGCAG TGGCACAGCT GGAGATTGAC 
CGGAGCAGGC TGCAGCACCA CAATGTCCAG CTGCGGGCCA CCTTGGAGCA GGTGGAGCGA 
GAGCGCAGGA AGCTGAAGAG GGAGTCCATG CGCGTGTCAC GGACAGGCGG CCTGGAGGTT 
AAGGAAGCTG CGGCTTCGTC CCCCACACAG CAGGATGGAA GGGGAGGACA GAAGGGCTCC 
TCAGATGACA AGCAGATGGC TGAACTGCAG AAAGAGGTGG CCATGCTGCG GGGCCAGCTG 
GCCTTGGAGC GGAAGCAGAG GCAGGACTAC ATTGCTCGCT CGGTGCAGAC TAGCCGTGAG 
CTAGCGGGCC TGCACCACAG CCTCTCCCAC TCACTGCTGG CCGTGGCCCA GGCCCCTGAA 
GCCACTGTCC TGGAGGCCGA GACCCGCAAG CTGGACGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGCTTTGCT GTGCCCCAGC CCCAGCACTA TCCAGGCCAT CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107039.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962514.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OCe42761
Clone ID Related Accession (Same CDS sequence)	XM_014107039.2 , XM_014107039.1
Accession Version	XM_014107039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962514.1
CDS	212..7525
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107039.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107039.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107039.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962514.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

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CloneID	OCe42761
Clone ID Related Accession (Same CDS sequence)	XM_014107039.2 , XM_014107039.1
Accession Version	XM_014107039.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962514.1
CDS	198..7511
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107039.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107039.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGATT 
TCTTCCTCTC AGGCACGTCA TCAGCAGGAG GCAGCCTTAG CTCAGCTGGA TCAGCTGCGT 
CAGGAGACAA AGCGACAGGA AGAAGTGCTT GCTCGAGAAG TCCAGGAGAA GGAGGCCTTA 
GTACGGGAGA GAGCAGCCCT AGAGGTGCGG CTGCAGGCCG TGGAGCGAGA CCGGCAGGAC 
CTCGCTGAAC AACTACTGGG CCTCAGCTCA GCCAAGGAGC AACTGGAGAG CACTCTGTTT 
GAGGCCCAAC AACAAAATTC TCTGGTAGAG GTCACGAAGG GACAGCTAGA GGTCCAGATT 
CAAACTGTCA TTCGAGCCAA GGAAGTAATT CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA 
CTGGACAGTG AGCGGAACCA GGCAGAACAA GAGCGGGAGA CAGCAGCCAG ACGGCTGGCC 
CAGGCCGAGC AAGAGGGGCA GACTGCCCTG CAGCAGCAGA AGTCAGCCCA CGAGGAGGAG 
GTGAACCGGC TCCAGGAGAA ATGGGAGAAG GAGCGCTCTT GGCACCAGCA GGAACTGGAT 
AAGGCCCTGG AGAGCCTAGA GAAGGAGAAA ATGGAGCTGG AAACGAGGCT GAGGGAACAG 
CAGGCAGAAG CCGAGGCCAT CCGGACACAG AGGGAGGAAG AACGGGCGGA GGCAGAGAGT 
GCCCTCTGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAG TGTCCCTCCT GGAGACCCTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TAGAGCGGCT GAGGCAGGAC 
ATGAAGGTCC AGAAGTTAAA GGAGCAGGAG ACCACTCAGA TCCTGCAGGC CCAGCTCCGG 
GAGGCTCGGG GGGAGCTGGA GCAGGCAGCC CAGCAGAACA GAGATGACCT TGTTGCTGTC 
CAAGAAGAGT GCGGGGCCCT GCTGCAGGCG AAGATGGACC TGCAGAAGCG GGTAGAAGAG 
TTGAAGTCTC AGCTGGTTTC CAGAGATGAC TCCCGGAGGC TGGTGGAGCA GGAGGTTCAG 
GAGAAGCTGA GGGAGGCCCA GGAGTATAGC CGAATTCAGA AGGAGCTGGA GAGAGAGAAA 
GCCAGCCTGA TTCAGTCGCT GATGGAAAAG GAGCAGAGAC TCCTTGTTTT ACAAGAAGCT 
GACTCTGTTC GACAACAGGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GTCCCAGGAA 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTTC TGGCTCAGGA AGCACAACTG CTGGAGGAGC TGGAGACATC TCAAGTAACA 
GAGCAGCGGC TGCGAGCTTC TTTGCGGGCC CTGGAAGCCA AGGCAGCCCA AGTCCAGCTG 
CGACTGCGCA GCACAGAGAA CCAGTTGGCA GCTCTGGTGG CAGAGCAGCA GCCGGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTCTGT TCTGTCCTGC AGCAGGCCTT GGGGTTTGCT 
TGTGAGAGCA GGCCTGAGCT GCGTGGCGGG GGAGACTCTG CTTCCCTCTG GGGCCCCGAG 
CCAGACCAGA ATGGAACTGG GATCCTCCTT AAGAGAGGGC CCCTCCTGAC AGCTCTGTCA 
GCTGAGGCAG TGGCATCTGC CCTCCAGAAA CTTCACCAAG ACCTATGGAA GACTCAGCAG 
GCCCGGGATG ATCTGCGGGA ACAGGCCCTG AAGCTGGAAC AGCGTCTCAC TGATACAGAG 
GCCGAGAAGA GTCAGGTCTG CACAGAATTG CAGGATTTGC AGAGACAACT CTCCCAGAAC 
CAGGAAGAGA AATCCAAGTG GGAAGGAAAA CAGATCTCCC TGGAATCTGA GCTGACTGAA 
CTGCATGGAA CTGTGGCATC ATTACAGAGT CGTCTACAGC AAGCAGAGCT GCAGGGACTA 
GAGGCCCAGA ATGAGCGAAA GCTACTGCAG GCAGCCAAGG AGAGCCTGAC AGCCCAGGTG 
GAACGTTTGC AGGCATCTGT GGCAGAAGCC AGGGCTCAGG CTGGTGCCAC CAGGGCTCTG 
GAGGAGGACT TGAGAACTGC TCGCTCAGCC CTGAAACTCA AGAGTGAGGA AGCAGAGACC 
GAGCGTGAGC GGGCCCAGGC TCTGCAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAGG AGAATTTAGC TATCCTGGTT CAGACTCTAT CCGAAAGAGA AGGGGAGGTG 
GAGGCTTTGC GGGGAAGTAT TCAGGAACTG GAGAAACAAC GGGAGATGCA AAAGGCTACT 
TTGGAAGCTC TGTCTCTGGA CCTGAAGAAG AAGAGCGAAG AGGTAGATGT GCAACAAGAA 
CAGATCCAGG AGCTGGAGAA GTGCAGGTCC CTTTTAGAAG ATCTGCCTCT GGCCATGCAG 
GAACAAGAGC AGAGGCTGGT TGCACAGAGG GAGCAAATCC AAGAGCTCGA GAAGGATCGA 
GAGACCCAGA GGAACATCTT GGAGCATCAG CTTCTCGAAC TTGAGAAGAA GGCCCAAATG 
ATAGAGTCCC AGAAAGGAGA GATTCAGGAC CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCTCTGG AACGAGAGGA AAACCATCAC AAGATGGAGT GCCAACAGAA GGCAATCGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGA GTAGCTCTGA CCCACCTCAC ACTGGACCTG 
GAAGAAAAGA GCCAGGAGCT GCAGGCCCAG AGCAGTCAGA TCGACAAGCT GGAGAGCCAT 
AGCACCCTTC TGGCGAAAGA GCTCCAGGAC AAGGACCAGG AGCTGAAGTC CCAGCAAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAGGAG CGTCTGGCTC AGGACCTAGA GAGGAGGGAC 
CAGGACGTGG TGCTCCAGAG GGAAAGGATT CGGGTCCTAG AAGACCAGAG GACACTGCAG 
ACCAAGATCC TGCAGGAGGA CCTGGGACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGGCTT CCCAGAGGCC ACCGATGCCG GAGTGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCCCAGC GCGGGAGCCT GGAGCACCTG AAGCTGATCC TGCGTGACAA GGAGAGGGAG 
GTAGAATGCC AGCAGGAACG CATCCAGGAA CTGAAGGAGT ATAAGGATCA GCTGGAGCAG 
CAGCTCCAGG GCCTACACAG GAAGGCAGGG GAGACTGGCC TCCTCCTGAC TCAGCGAGAG 
CAGGAGATAG TGGTCCTGCA GCGGCATCTG CAGGAAGCCG CAGAACAGGG GGAGCTGAAA 
GAGCGGTCAC TTCAGGGTCA CCTGGAAGAG GCCCAGAGAG CCCTGGCCCA GAGGGACCAG 
GAGCTCGAGG CCCTGCAGCA CCAACAGCAG CAGGCCCTGG GGCAGGAGGA GACTAGGAAG 
GAAGAGGCAA GCACCCTGCA GAGGGCTCTG GAGCAGGCCC ACACGGCGCT GAAAGAGCGC 
CAGGGAGAGC TTGAGGACCA CAAGGAGCAT GTGCGAAGGC TCCAGGAGGA GCTGGCCGTG 
GAGGGACGGC GTGTGCAGGC CCTGGAGGAG GTGGTGGGTG ACCTAAGGGC TGAGTCTCGG 
GAGCAGGAGG AGGCTTTGCT GGCCCTCCAG CAACAGGGTG CCGAGCGGGC ACAGGAGCAT 
GAGGCGGAGG TCAGGGGCCT GCGGGCCAGC CTCCTGCAGG CAGAGACTGC ACTCAAGGAA 
CGGGACCTGG CGCTGGAGGC CCTGCGAGCC GATGGCCGGG CCTCCCAGCT TCGGGAGGAG 
ACAGCCCGGG ACTGGGCCCA AGCTCTGCAG GAGGCCCTGA GCAAGGCCCA GGCTGCCGTG 
CAGGAGAAAG AGCAGCGGCT GCTGGGTCAA GCAGAGTTGA GCCGCAGCCT GGAGACCAGC 
ACCGCCACTT TGCAGGCTGC CCTGGACTCT TGCCAGGCAC AAGCCCGGCA GCTGGAGGAG 
GCTCTGAGGA GGCGAGAGGA CGAGATCCAG GACCGGGACC TCCGGCACCA GGAGGCCGTG 
CAGCAGCTCC AGCGGGCACT TGCCCAGAGA GATGAAGAAT TGAGCCATCA GAAGAGACAG 
GGGCAGCTGC TAGAGCAGTC TCTGGCCCGG AGGGCCCGAA AAGATGCCAT CCAAGGGAAG 
CCAGGTCCGG AGCAGGAGAA AGAGGAAGAG ATGAGAGGCC TTCGAGAAAG CCTAAGGGAG 
TTGCAGCTGA CTCTAGCCCA AAAGGAAGAG GAGATCCTGG GGCTGAGGGA GGCCCAGCAA 
AGGAAGAATC TGGAGGACTC ACTGCACAGC CACACAGCGC CCCCAGAGCC CTCTACAGAC 
TTTGCCACCT TAGGGCCCAG GCTGCAGCAG GAGCTGGAGC GACTGCAGAC AGCACTGAGG 
CTAACTGAGG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTGTCCCTG 
GCCCAGAGCA AGGCTAGCGT CAGCAGTTTG CAGGAGGCAG CCATGTTCCT ACAGGCCTCT 
GTTCTGGAGC GGGACTTGGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAGACAG 
GCTCTGGAGA AGGAGCAGCT CCTGAGCTCT AGTTCAACCA GCAGAGCAGA ACAGAGGCCC 
AGAGAAGAGG TGTCAGAAGT CGAGGCTGAG CCTAGTCTTG GGCTGGAGGA GAGGCAGCTC 
TGGGGACAAA GGCTGGAGTA CCTCCAGCAA GCAGTGGCAC AGCTGGAGAT TGACCGGAGC 
AGGCTGCAGC ACCACAATGT CCAGCTGCGG GCCACCTTGG AGCAGGTGGA GCGAGAGCGC 
AGGAAGCTGA AGAGGGAGTC CATGCGCGTG TCACGGACAG GCGGCCTGGA GGTTAAGGAA 
GCTGCGGCTT CGTCCCCCAC ACAGCAGGAT GGAAGGGGAG GACAGAAGGG CTCCTCAGAT 
GACAAGCAGA TGGCTGAACT GCAGAAAGAG GTGGCCATGC TGCGGGGCCA GCTGGCCTTG 
GAGCGGAAGC AGAGGCAGGA CTACATTGCT CGCTCGGTGC AGACTAGCCG TGAGCTAGCG 
GGCCTGCACC ACAGCCTCTC CCACTCACTG CTGGCCGTGG CCCAGGCCCC TGAAGCCACT 
GTCCTGGAGG CCGAGACCCG CAAGCTGGAC GAGTCCCTGA CTCAAAGTCT GACATCCCCA 
GGGCCAGCTT TGCTGTGCCC CAGCCCCAGC ACTATCCAGG CCATCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107040.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962515.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

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CloneID	OCe42762
Clone ID Related Accession (Same CDS sequence)	XM_014107040.2 , XM_014107040.1
Accession Version	XM_014107040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGGCA 
CGTCATCAGC AGGAGGCAGC CTTAGCTCAG CTGGATCAGC TGCGTCAGGA GACAAAGCGA 
CAGGAAGAAG TGCTTGCTCG AGAAGTCCAG GAGAAGGAGG CCTTAGTACG GGAGAGAGCA 
GCCCTAGAGG TGCGGCTGCA GGCCGTGGAG CGAGACCGGC AGGACCTCGC TGAACAACTA 
CTGGGCCTCA GCTCAGCCAA GGAGCAACTG GAGAGCACTC TGTTTGAGGC CCAACAACAA 
AATTCTCTGG TAGAGGTCAC GAAGGGACAG CTAGAGGTCC AGATTCAAAC TGTCATTCGA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAGG TGCCTGAAGC TGGAACTGGA CAGTGAGCGG 
AACCAGGCAG AACAAGAGCG GGAGACAGCA GCCAGACGGC TGGCCCAGGC CGAGCAAGAG 
GGGCAGACTG CCCTGCAGCA GCAGAAGTCA GCCCACGAGG AGGAGGTGAA CCGGCTCCAG 
GAGAAATGGG AGAAGGAGCG CTCTTGGCAC CAGCAGGAAC TGGATAAGGC CCTGGAGAGC 
CTAGAGAAGG AGAAAATGGA GCTGGAAACG AGGCTGAGGG AACAGCAGGC AGAAGCCGAG 
GCCATCCGGA CACAGAGGGA GGAAGAACGG GCGGAGGCAG AGAGTGCCCT CTGCCAGATG 
CAGCTCGAAA CAGAGAAGGA GAGAGTGTCC CTCCTGGAGA CCCTGCTGCA GACCCAGAAG 
GAGCTGGCAG ATGCCAGCCA ACAACTAGAG CGGCTGAGGC AGGACATGAA GGTCCAGAAG 
TTAAAGGAGC AGGAGACCAC TCAGATCCTG CAGGCCCAGC TCCGGGAGGC TCGGGGGGAG 
CTGGAGCAGG CAGCCCAGCA GAACAGAGAT GACCTTGTTG CTGTCCAAGA AGAGTGCGGG 
GCCCTGCTGC AGGCGAAGAT GGACCTGCAG AAGCGGGTAG AAGAGTTGAA GTCTCAGCTG 
GTTTCCAGAG ATGACTCCCG GAGGCTGGTG GAGCAGGAGG TTCAGGAGAA GCTGAGGGAG 
GCCCAGGAGT ATAGCCGAAT TCAGAAGGAG CTGGAGAGAG AGAAAGCCAG CCTGATTCAG 
TCGCTGATGG AAAAGGAGCA GAGACTCCTT GTTTTACAAG AAGCTGACTC TGTTCGACAA 
CAGGAGCTGA GCTCCCTGCG CCAGGACATG CAGGAGTCCC AGGAAGGGCA GAAAGAGCTC 
AGCACCCAGG TGGAATTACT GAAGCAGGAG GTGAAGGAAA AGGAGGCTGA CTTTCTGGCT 
CAGGAAGCAC AACTGCTGGA GGAGCTGGAG ACATCTCAAG TAACAGAGCA GCGGCTGCGA 
GCTTCTTTGC GGGCCCTGGA AGCCAAGGCA GCCCAAGTCC AGCTGCGACT GCGCAGCACA 
GAGAACCAGT TGGCAGCTCT GGTGGCAGAG CAGCAGCCGG GGCACCAGGC CCAGGCCCAG 
CTGGCCAGCC TCTGTTCTGT CCTGCAGCAG GCCTTGGGGT TTGCTTGTGA GAGCAGGCCT 
GAGCTGCGTG GCGGGGGAGA CTCTGCTTCC CTCTGGGGCC CCGAGCCAGA CCAGAATGGA 
ACTGGGATCC TCCTTAAGAG AGGGCCCCTC CTGACAGCTC TGTCAGCTGA GGCAGTGGCA 
TCTGCCCTCC AGAAACTTCA CCAAGACCTA TGGAAGACTC AGCAGGCCCG GGATGATCTG 
CGGGAACAGG CCCTGAAGCT GGAACAGCGT CTCACTGATA CAGAGGCCGA GAAGAGTCAG 
GTCTGCACAG AATTGCAGGA TTTGCAGAGA CAACTCTCCC AGAACCAGGA AGAGAAATCC 
AAGTGGGAAG GAAAACAGAT CTCCCTGGAA TCTGAGCTGA CTGAACTGCA TGGAACTGTG 
GCATCATTAC AGAGTCGTCT ACAGCAAGCA GAGCTGCAGG GACTAGAGGC CCAGAATGAG 
CGAAAGCTAC TGCAGGCAGC CAAGGAGAGC CTGACAGCCC AGGTGGAACG TTTGCAGGCA 
TCTGTGGCAG AAGCCAGGGC TCAGGCTGGT GCCACCAGGG CTCTGGAGGA GGACTTGAGA 
ACTGCTCGCT CAGCCCTGAA ACTCAAGAGT GAGGAAGCAG AGACCGAGCG TGAGCGGGCC 
CAGGCTCTGC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAGGCTCT GCAGGAGAAT 
TTAGCTATCC TGGTTCAGAC TCTATCCGAA AGAGAAGGGG AGGTGGAGGC TTTGCGGGGA 
AGTATTCAGG AACTGGAGAA ACAACGGGAG ATGCAAAAGG CTACTTTGGA AGCTCTGTCT 
CTGGACCTGA AGAAGAAGAG CGAAGAGGTA GATGTGCAAC AAGAACAGAT CCAGGAGCTG 
GAGAAGTGCA GGTCCCTTTT AGAAGATCTG CCTCTGGCCA TGCAGGAACA AGAGCAGAGG 
CTGGTTGCAC AGAGGGAGCA AATCCAAGAG CTCGAGAAGG ATCGAGAGAC CCAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT CGAACTTGAG AAGAAGGCCC AAATGATAGA GTCCCAGAAA 
GGAGAGATTC AGGACCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC TCTGGAACGA 
GAGGAAAACC ATCACAAGAT GGAGTGCCAA CAGAAGGCAA TCGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGAGTAGC TCTGACCCAC CTCACACTGG ACCTGGAAGA AAAGAGCCAG 
GAGCTGCAGG CCCAGAGCAG TCAGATCGAC AAGCTGGAGA GCCATAGCAC CCTTCTGGCG 
AAAGAGCTCC AGGACAAGGA CCAGGAGCTG AAGTCCCAGC AAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AGGAGCGTCT GGCTCAGGAC CTAGAGAGGA GGGACCAGGA CGTGGTGCTC 
CAGAGGGAAA GGATTCGGGT CCTAGAAGAC CAGAGGACAC TGCAGACCAA GATCCTGCAG 
GAGGACCTGG GACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GGCTTCCCAG 
AGGCCACCGA TGCCGGAGTG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC CCAGCGCGGG 
AGCCTGGAGC ACCTGAAGCT GATCCTGCGT GACAAGGAGA GGGAGGTAGA ATGCCAGCAG 
GAACGCATCC AGGAACTGAA GGAGTATAAG GATCAGCTGG AGCAGCAGCT CCAGGGCCTA 
CACAGGAAGG CAGGGGAGAC TGGCCTCCTC CTGACTCAGC GAGAGCAGGA GATAGTGGTC 
CTGCAGCGGC ATCTGCAGGA AGCCGCAGAA CAGGGGGAGC TGAAAGAGCG GTCACTTCAG 
GGTCACCTGG AAGAGGCCCA GAGAGCCCTG GCCCAGAGGG ACCAGGAGCT CGAGGCCCTG 
CAGCACCAAC AGCAGCAGGC CCTGGGGCAG GAGGAGACTA GGAAGGAAGA GGCAAGCACC 
CTGCAGAGGG CTCTGGAGCA GGCCCACACG GCGCTGAAAG AGCGCCAGGG AGAGCTTGAG 
GACCACAAGG AGCATGTGCG AAGGCTCCAG GAGGAGCTGG CCGTGGAGGG ACGGCGTGTG 
CAGGCCCTGG AGGAGGTGGT GGGTGACCTA AGGGCTGAGT CTCGGGAGCA GGAGGAGGCT 
TTGCTGGCCC TCCAGCAACA GGGTGCCGAG CGGGCACAGG AGCATGAGGC GGAGGTCAGG 
GGCCTGCGGG CCAGCCTCCT GCAGGCAGAG ACTGCACTCA AGGAACGGGA CCTGGCGCTG 
GAGGCCCTGC GAGCCGATGG CCGGGCCTCC CAGCTTCGGG AGGAGACAGC CCGGGACTGG 
GCCCAAGCTC TGCAGGAGGC CCTGAGCAAG GCCCAGGCTG CCGTGCAGGA GAAAGAGCAG 
CGGCTGCTGG GTCAAGCAGA GTTGAGCCGC AGCCTGGAGA CCAGCACCGC CACTTTGCAG 
GCTGCCCTGG ACTCTTGCCA GGCACAAGCC CGGCAGCTGG AGGAGGCTCT GAGGAGGCGA 
GAGGACGAGA TCCAGGACCG GGACCTCCGG CACCAGGAGG CCGTGCAGCA GCTCCAGCGG 
GCACTTGCCC AGAGAGATGA AGAATTGAGC CATCAGAAGA GACAGGGGCA GCTGCTAGAG 
CAGTCTCTGG CCCGGAGGGC CCGAAAAGAT GCCATCCAAG GGAAGCCAGG TCCGGAGCAG 
GAGAAAGAGG AAGAGATGAG AGGCCTTCGA GAAAGCCTAA GGGAGTTGCA GCTGACTCTA 
GCCCAAAAGG AAGAGGAGAT CCTGGGGCTG AGGGAGGCCC AGCAAAGGAA GAATCTGGAG 
GACTCACTGC ACAGCCACAC AGCGCCCCCA GAGCCCTCTA CAGACTTTGC CACCTTAGGG 
CCCAGGCTGC AGCAGGAGCT GGAGCGACTG CAGACAGCAC TGAGGCTAAC TGAGGCCAGG 
GAGATTGAGT GGAGGGAGAA GGCCCAGGAC TTGGCACTGT CCCTGGCCCA GAGCAAGGCT 
AGCGTCAGCA GTTTGCAGGA GGCAGCCATG TTCCTACAGG CCTCTGTTCT GGAGCGGGAC 
TTGGAACAGC AGAGGCTGCA GGATGAGTTG GAGCTCACCA GACAGGCTCT GGAGAAGGAG 
CAGCTCCTGA GCTCTAGTTC AACCAGCAGA GCAGAACAGA GGCCCAGAGA AGAGGTGTCA 
GAAGTCGAGG CTGAGCCTAG TCTTGGGCTG GAGGAGAGGC AGCTCTGGGG ACAAAGGCTG 
GAGTACCTCC AGCAAGCAGT GGCACAGCTG GAGATTGACC GGAGCAGGCT GCAGCACCAC 
AATGTCCAGC TGCGGGCCAC CTTGGAGCAG GTGGAGCGAG AGCGCAGGAA GCTGAAGAGG 
GAGTCCATGC GCGTGTCACG GACAGGCGGC CTGGAGGTTA AGGAAGCTGC GGCTTCGTCC 
CCCACACAGC AGGATGGAAG GGGAGGACAG AAGGGCTCCT CAGATGACAA GCAGATGGCT 
GAACTGCAGA AAGAGGTGGC CATGCTGCGG GGCCAGCTGG CCTTGGAGCG GAAGCAGAGG 
CAGGACTACA TTGCTCGCTC GGTGCAGACT AGCCGTGAGC TAGCGGGCCT GCACCACAGC 
CTCTCCCACT CACTGCTGGC CGTGGCCCAG GCCCCTGAAG CCACTGTCCT GGAGGCCGAG 
ACCCGCAAGC TGGACGAGTC CCTGACTCAA AGTCTGACAT CCCCAGGGCC AGCTTTGCTG 
TGCCCCAGCC CCAGCACTAT CCAGGCCATC TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962515.1
CDS	212..7510
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107040.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107040.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGGCA 
CGTCATCAGC AGGAGGCAGC CTTAGCTCAG CTGGATCAGC TGCGTCAGGA GACAAAGCGA 
CAGGAAGAAG TGCTTGCTCG AGAAGTCCAG GAGAAGGAGG CCTTAGTACG GGAGAGAGCA 
GCCCTAGAGG TGCGGCTGCA GGCCGTGGAG CGAGACCGGC AGGACCTCGC TGAACAACTA 
CTGGGCCTCA GCTCAGCCAA GGAGCAACTG GAGAGCACTC TGTTTGAGGC CCAACAACAA 
AATTCTCTGG TAGAGGTCAC GAAGGGACAG CTAGAGGTCC AGATTCAAAC TGTCATTCGA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAGG TGCCTGAAGC TGGAACTGGA CAGTGAGCGG 
AACCAGGCAG AACAAGAGCG GGAGACAGCA GCCAGACGGC TGGCCCAGGC CGAGCAAGAG 
GGGCAGACTG CCCTGCAGCA GCAGAAGTCA GCCCACGAGG AGGAGGTGAA CCGGCTCCAG 
GAGAAATGGG AGAAGGAGCG CTCTTGGCAC CAGCAGGAAC TGGATAAGGC CCTGGAGAGC 
CTAGAGAAGG AGAAAATGGA GCTGGAAACG AGGCTGAGGG AACAGCAGGC AGAAGCCGAG 
GCCATCCGGA CACAGAGGGA GGAAGAACGG GCGGAGGCAG AGAGTGCCCT CTGCCAGATG 
CAGCTCGAAA CAGAGAAGGA GAGAGTGTCC CTCCTGGAGA CCCTGCTGCA GACCCAGAAG 
GAGCTGGCAG ATGCCAGCCA ACAACTAGAG CGGCTGAGGC AGGACATGAA GGTCCAGAAG 
TTAAAGGAGC AGGAGACCAC TCAGATCCTG CAGGCCCAGC TCCGGGAGGC TCGGGGGGAG 
CTGGAGCAGG CAGCCCAGCA GAACAGAGAT GACCTTGTTG CTGTCCAAGA AGAGTGCGGG 
GCCCTGCTGC AGGCGAAGAT GGACCTGCAG AAGCGGGTAG AAGAGTTGAA GTCTCAGCTG 
GTTTCCAGAG ATGACTCCCG GAGGCTGGTG GAGCAGGAGG TTCAGGAGAA GCTGAGGGAG 
GCCCAGGAGT ATAGCCGAAT TCAGAAGGAG CTGGAGAGAG AGAAAGCCAG CCTGATTCAG 
TCGCTGATGG AAAAGGAGCA GAGACTCCTT GTTTTACAAG AAGCTGACTC TGTTCGACAA 
CAGGAGCTGA GCTCCCTGCG CCAGGACATG CAGGAGTCCC AGGAAGGGCA GAAAGAGCTC 
AGCACCCAGG TGGAATTACT GAAGCAGGAG GTGAAGGAAA AGGAGGCTGA CTTTCTGGCT 
CAGGAAGCAC AACTGCTGGA GGAGCTGGAG ACATCTCAAG TAACAGAGCA GCGGCTGCGA 
GCTTCTTTGC GGGCCCTGGA AGCCAAGGCA GCCCAAGTCC AGCTGCGACT GCGCAGCACA 
GAGAACCAGT TGGCAGCTCT GGTGGCAGAG CAGCAGCCGG GGCACCAGGC CCAGGCCCAG 
CTGGCCAGCC TCTGTTCTGT CCTGCAGCAG GCCTTGGGGT TTGCTTGTGA GAGCAGGCCT 
GAGCTGCGTG GCGGGGGAGA CTCTGCTTCC CTCTGGGGCC CCGAGCCAGA CCAGAATGGA 
ACTGGGATCC TCCTTAAGAG AGGGCCCCTC CTGACAGCTC TGTCAGCTGA GGCAGTGGCA 
TCTGCCCTCC AGAAACTTCA CCAAGACCTA TGGAAGACTC AGCAGGCCCG GGATGATCTG 
CGGGAACAGG CCCTGAAGCT GGAACAGCGT CTCACTGATA CAGAGGCCGA GAAGAGTCAG 
GTCTGCACAG AATTGCAGGA TTTGCAGAGA CAACTCTCCC AGAACCAGGA AGAGAAATCC 
AAGTGGGAAG GAAAACAGAT CTCCCTGGAA TCTGAGCTGA CTGAACTGCA TGGAACTGTG 
GCATCATTAC AGAGTCGTCT ACAGCAAGCA GAGCTGCAGG GACTAGAGGC CCAGAATGAG 
CGAAAGCTAC TGCAGGCAGC CAAGGAGAGC CTGACAGCCC AGGTGGAACG TTTGCAGGCA 
TCTGTGGCAG AAGCCAGGGC TCAGGCTGGT GCCACCAGGG CTCTGGAGGA GGACTTGAGA 
ACTGCTCGCT CAGCCCTGAA ACTCAAGAGT GAGGAAGCAG AGACCGAGCG TGAGCGGGCC 
CAGGCTCTGC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAGGCTCT GCAGGAGAAT 
TTAGCTATCC TGGTTCAGAC TCTATCCGAA AGAGAAGGGG AGGTGGAGGC TTTGCGGGGA 
AGTATTCAGG AACTGGAGAA ACAACGGGAG ATGCAAAAGG CTACTTTGGA AGCTCTGTCT 
CTGGACCTGA AGAAGAAGAG CGAAGAGGTA GATGTGCAAC AAGAACAGAT CCAGGAGCTG 
GAGAAGTGCA GGTCCCTTTT AGAAGATCTG CCTCTGGCCA TGCAGGAACA AGAGCAGAGG 
CTGGTTGCAC AGAGGGAGCA AATCCAAGAG CTCGAGAAGG ATCGAGAGAC CCAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT CGAACTTGAG AAGAAGGCCC AAATGATAGA GTCCCAGAAA 
GGAGAGATTC AGGACCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC TCTGGAACGA 
GAGGAAAACC ATCACAAGAT GGAGTGCCAA CAGAAGGCAA TCGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGAGTAGC TCTGACCCAC CTCACACTGG ACCTGGAAGA AAAGAGCCAG 
GAGCTGCAGG CCCAGAGCAG TCAGATCGAC AAGCTGGAGA GCCATAGCAC CCTTCTGGCG 
AAAGAGCTCC AGGACAAGGA CCAGGAGCTG AAGTCCCAGC AAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AGGAGCGTCT GGCTCAGGAC CTAGAGAGGA GGGACCAGGA CGTGGTGCTC 
CAGAGGGAAA GGATTCGGGT CCTAGAAGAC CAGAGGACAC TGCAGACCAA GATCCTGCAG 
GAGGACCTGG GACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GGCTTCCCAG 
AGGCCACCGA TGCCGGAGTG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC CCAGCGCGGG 
AGCCTGGAGC ACCTGAAGCT GATCCTGCGT GACAAGGAGA GGGAGGTAGA ATGCCAGCAG 
GAACGCATCC AGGAACTGAA GGAGTATAAG GATCAGCTGG AGCAGCAGCT CCAGGGCCTA 
CACAGGAAGG CAGGGGAGAC TGGCCTCCTC CTGACTCAGC GAGAGCAGGA GATAGTGGTC 
CTGCAGCGGC ATCTGCAGGA AGCCGCAGAA CAGGGGGAGC TGAAAGAGCG GTCACTTCAG 
GGTCACCTGG AAGAGGCCCA GAGAGCCCTG GCCCAGAGGG ACCAGGAGCT CGAGGCCCTG 
CAGCACCAAC AGCAGCAGGC CCTGGGGCAG GAGGAGACTA GGAAGGAAGA GGCAAGCACC 
CTGCAGAGGG CTCTGGAGCA GGCCCACACG GCGCTGAAAG AGCGCCAGGG AGAGCTTGAG 
GACCACAAGG AGCATGTGCG AAGGCTCCAG GAGGAGCTGG CCGTGGAGGG ACGGCGTGTG 
CAGGCCCTGG AGGAGGTGGT GGGTGACCTA AGGGCTGAGT CTCGGGAGCA GGAGGAGGCT 
TTGCTGGCCC TCCAGCAACA GGGTGCCGAG CGGGCACAGG AGCATGAGGC GGAGGTCAGG 
GGCCTGCGGG CCAGCCTCCT GCAGGCAGAG ACTGCACTCA AGGAACGGGA CCTGGCGCTG 
GAGGCCCTGC GAGCCGATGG CCGGGCCTCC CAGCTTCGGG AGGAGACAGC CCGGGACTGG 
GCCCAAGCTC TGCAGGAGGC CCTGAGCAAG GCCCAGGCTG CCGTGCAGGA GAAAGAGCAG 
CGGCTGCTGG GTCAAGCAGA GTTGAGCCGC AGCCTGGAGA CCAGCACCGC CACTTTGCAG 
GCTGCCCTGG ACTCTTGCCA GGCACAAGCC CGGCAGCTGG AGGAGGCTCT GAGGAGGCGA 
GAGGACGAGA TCCAGGACCG GGACCTCCGG CACCAGGAGG CCGTGCAGCA GCTCCAGCGG 
GCACTTGCCC AGAGAGATGA AGAATTGAGC CATCAGAAGA GACAGGGGCA GCTGCTAGAG 
CAGTCTCTGG CCCGGAGGGC CCGAAAAGAT GCCATCCAAG GGAAGCCAGG TCCGGAGCAG 
GAGAAAGAGG AAGAGATGAG AGGCCTTCGA GAAAGCCTAA GGGAGTTGCA GCTGACTCTA 
GCCCAAAAGG AAGAGGAGAT CCTGGGGCTG AGGGAGGCCC AGCAAAGGAA GAATCTGGAG 
GACTCACTGC ACAGCCACAC AGCGCCCCCA GAGCCCTCTA CAGACTTTGC CACCTTAGGG 
CCCAGGCTGC AGCAGGAGCT GGAGCGACTG CAGACAGCAC TGAGGCTAAC TGAGGCCAGG 
GAGATTGAGT GGAGGGAGAA GGCCCAGGAC TTGGCACTGT CCCTGGCCCA GAGCAAGGCT 
AGCGTCAGCA GTTTGCAGGA GGCAGCCATG TTCCTACAGG CCTCTGTTCT GGAGCGGGAC 
TTGGAACAGC AGAGGCTGCA GGATGAGTTG GAGCTCACCA GACAGGCTCT GGAGAAGGAG 
CAGCTCCTGA GCTCTAGTTC AACCAGCAGA GCAGAACAGA GGCCCAGAGA AGAGGTGTCA 
GAAGTCGAGG CTGAGCCTAG TCTTGGGCTG GAGGAGAGGC AGCTCTGGGG ACAAAGGCTG 
GAGTACCTCC AGCAAGCAGT GGCACAGCTG GAGATTGACC GGAGCAGGCT GCAGCACCAC 
AATGTCCAGC TGCGGGCCAC CTTGGAGCAG GTGGAGCGAG AGCGCAGGAA GCTGAAGAGG 
GAGTCCATGC GCGTGTCACG GACAGGCGGC CTGGAGGTTA AGGAAGCTGC GGCTTCGTCC 
CCCACACAGC AGGATGGAAG GGGAGGACAG AAGGGCTCCT CAGATGACAA GCAGATGGCT 
GAACTGCAGA AAGAGGTGGC CATGCTGCGG GGCCAGCTGG CCTTGGAGCG GAAGCAGAGG 
CAGGACTACA TTGCTCGCTC GGTGCAGACT AGCCGTGAGC TAGCGGGCCT GCACCACAGC 
CTCTCCCACT CACTGCTGGC CGTGGCCCAG GCCCCTGAAG CCACTGTCCT GGAGGCCGAG 
ACCCGCAAGC TGGACGAGTC CCTGACTCAA AGTCTGACAT CCCCAGGGCC AGCTTTGCTG 
TGCCCCAGCC CCAGCACTAT CCAGGCCATC TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107040.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962515.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

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CloneID	OCe42762
Clone ID Related Accession (Same CDS sequence)	XM_014107040.2 , XM_014107040.1
Accession Version	XM_014107040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGGCA 
CGTCATCAGC AGGAGGCAGC CTTAGCTCAG CTGGATCAGC TGCGTCAGGA GACAAAGCGA 
CAGGAAGAAG TGCTTGCTCG AGAAGTCCAG GAGAAGGAGG CCTTAGTACG GGAGAGAGCA 
GCCCTAGAGG TGCGGCTGCA GGCCGTGGAG CGAGACCGGC AGGACCTCGC TGAACAACTA 
CTGGGCCTCA GCTCAGCCAA GGAGCAACTG GAGAGCACTC TGTTTGAGGC CCAACAACAA 
AATTCTCTGG TAGAGGTCAC GAAGGGACAG CTAGAGGTCC AGATTCAAAC TGTCATTCGA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAGG TGCCTGAAGC TGGAACTGGA CAGTGAGCGG 
AACCAGGCAG AACAAGAGCG GGAGACAGCA GCCAGACGGC TGGCCCAGGC CGAGCAAGAG 
GGGCAGACTG CCCTGCAGCA GCAGAAGTCA GCCCACGAGG AGGAGGTGAA CCGGCTCCAG 
GAGAAATGGG AGAAGGAGCG CTCTTGGCAC CAGCAGGAAC TGGATAAGGC CCTGGAGAGC 
CTAGAGAAGG AGAAAATGGA GCTGGAAACG AGGCTGAGGG AACAGCAGGC AGAAGCCGAG 
GCCATCCGGA CACAGAGGGA GGAAGAACGG GCGGAGGCAG AGAGTGCCCT CTGCCAGATG 
CAGCTCGAAA CAGAGAAGGA GAGAGTGTCC CTCCTGGAGA CCCTGCTGCA GACCCAGAAG 
GAGCTGGCAG ATGCCAGCCA ACAACTAGAG CGGCTGAGGC AGGACATGAA GGTCCAGAAG 
TTAAAGGAGC AGGAGACCAC TCAGATCCTG CAGGCCCAGC TCCGGGAGGC TCGGGGGGAG 
CTGGAGCAGG CAGCCCAGCA GAACAGAGAT GACCTTGTTG CTGTCCAAGA AGAGTGCGGG 
GCCCTGCTGC AGGCGAAGAT GGACCTGCAG AAGCGGGTAG AAGAGTTGAA GTCTCAGCTG 
GTTTCCAGAG ATGACTCCCG GAGGCTGGTG GAGCAGGAGG TTCAGGAGAA GCTGAGGGAG 
GCCCAGGAGT ATAGCCGAAT TCAGAAGGAG CTGGAGAGAG AGAAAGCCAG CCTGATTCAG 
TCGCTGATGG AAAAGGAGCA GAGACTCCTT GTTTTACAAG AAGCTGACTC TGTTCGACAA 
CAGGAGCTGA GCTCCCTGCG CCAGGACATG CAGGAGTCCC AGGAAGGGCA GAAAGAGCTC 
AGCACCCAGG TGGAATTACT GAAGCAGGAG GTGAAGGAAA AGGAGGCTGA CTTTCTGGCT 
CAGGAAGCAC AACTGCTGGA GGAGCTGGAG ACATCTCAAG TAACAGAGCA GCGGCTGCGA 
GCTTCTTTGC GGGCCCTGGA AGCCAAGGCA GCCCAAGTCC AGCTGCGACT GCGCAGCACA 
GAGAACCAGT TGGCAGCTCT GGTGGCAGAG CAGCAGCCGG GGCACCAGGC CCAGGCCCAG 
CTGGCCAGCC TCTGTTCTGT CCTGCAGCAG GCCTTGGGGT TTGCTTGTGA GAGCAGGCCT 
GAGCTGCGTG GCGGGGGAGA CTCTGCTTCC CTCTGGGGCC CCGAGCCAGA CCAGAATGGA 
ACTGGGATCC TCCTTAAGAG AGGGCCCCTC CTGACAGCTC TGTCAGCTGA GGCAGTGGCA 
TCTGCCCTCC AGAAACTTCA CCAAGACCTA TGGAAGACTC AGCAGGCCCG GGATGATCTG 
CGGGAACAGG CCCTGAAGCT GGAACAGCGT CTCACTGATA CAGAGGCCGA GAAGAGTCAG 
GTCTGCACAG AATTGCAGGA TTTGCAGAGA CAACTCTCCC AGAACCAGGA AGAGAAATCC 
AAGTGGGAAG GAAAACAGAT CTCCCTGGAA TCTGAGCTGA CTGAACTGCA TGGAACTGTG 
GCATCATTAC AGAGTCGTCT ACAGCAAGCA GAGCTGCAGG GACTAGAGGC CCAGAATGAG 
CGAAAGCTAC TGCAGGCAGC CAAGGAGAGC CTGACAGCCC AGGTGGAACG TTTGCAGGCA 
TCTGTGGCAG AAGCCAGGGC TCAGGCTGGT GCCACCAGGG CTCTGGAGGA GGACTTGAGA 
ACTGCTCGCT CAGCCCTGAA ACTCAAGAGT GAGGAAGCAG AGACCGAGCG TGAGCGGGCC 
CAGGCTCTGC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAGGCTCT GCAGGAGAAT 
TTAGCTATCC TGGTTCAGAC TCTATCCGAA AGAGAAGGGG AGGTGGAGGC TTTGCGGGGA 
AGTATTCAGG AACTGGAGAA ACAACGGGAG ATGCAAAAGG CTACTTTGGA AGCTCTGTCT 
CTGGACCTGA AGAAGAAGAG CGAAGAGGTA GATGTGCAAC AAGAACAGAT CCAGGAGCTG 
GAGAAGTGCA GGTCCCTTTT AGAAGATCTG CCTCTGGCCA TGCAGGAACA AGAGCAGAGG 
CTGGTTGCAC AGAGGGAGCA AATCCAAGAG CTCGAGAAGG ATCGAGAGAC CCAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT CGAACTTGAG AAGAAGGCCC AAATGATAGA GTCCCAGAAA 
GGAGAGATTC AGGACCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC TCTGGAACGA 
GAGGAAAACC ATCACAAGAT GGAGTGCCAA CAGAAGGCAA TCGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGAGTAGC TCTGACCCAC CTCACACTGG ACCTGGAAGA AAAGAGCCAG 
GAGCTGCAGG CCCAGAGCAG TCAGATCGAC AAGCTGGAGA GCCATAGCAC CCTTCTGGCG 
AAAGAGCTCC AGGACAAGGA CCAGGAGCTG AAGTCCCAGC AAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AGGAGCGTCT GGCTCAGGAC CTAGAGAGGA GGGACCAGGA CGTGGTGCTC 
CAGAGGGAAA GGATTCGGGT CCTAGAAGAC CAGAGGACAC TGCAGACCAA GATCCTGCAG 
GAGGACCTGG GACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GGCTTCCCAG 
AGGCCACCGA TGCCGGAGTG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC CCAGCGCGGG 
AGCCTGGAGC ACCTGAAGCT GATCCTGCGT GACAAGGAGA GGGAGGTAGA ATGCCAGCAG 
GAACGCATCC AGGAACTGAA GGAGTATAAG GATCAGCTGG AGCAGCAGCT CCAGGGCCTA 
CACAGGAAGG CAGGGGAGAC TGGCCTCCTC CTGACTCAGC GAGAGCAGGA GATAGTGGTC 
CTGCAGCGGC ATCTGCAGGA AGCCGCAGAA CAGGGGGAGC TGAAAGAGCG GTCACTTCAG 
GGTCACCTGG AAGAGGCCCA GAGAGCCCTG GCCCAGAGGG ACCAGGAGCT CGAGGCCCTG 
CAGCACCAAC AGCAGCAGGC CCTGGGGCAG GAGGAGACTA GGAAGGAAGA GGCAAGCACC 
CTGCAGAGGG CTCTGGAGCA GGCCCACACG GCGCTGAAAG AGCGCCAGGG AGAGCTTGAG 
GACCACAAGG AGCATGTGCG AAGGCTCCAG GAGGAGCTGG CCGTGGAGGG ACGGCGTGTG 
CAGGCCCTGG AGGAGGTGGT GGGTGACCTA AGGGCTGAGT CTCGGGAGCA GGAGGAGGCT 
TTGCTGGCCC TCCAGCAACA GGGTGCCGAG CGGGCACAGG AGCATGAGGC GGAGGTCAGG 
GGCCTGCGGG CCAGCCTCCT GCAGGCAGAG ACTGCACTCA AGGAACGGGA CCTGGCGCTG 
GAGGCCCTGC GAGCCGATGG CCGGGCCTCC CAGCTTCGGG AGGAGACAGC CCGGGACTGG 
GCCCAAGCTC TGCAGGAGGC CCTGAGCAAG GCCCAGGCTG CCGTGCAGGA GAAAGAGCAG 
CGGCTGCTGG GTCAAGCAGA GTTGAGCCGC AGCCTGGAGA CCAGCACCGC CACTTTGCAG 
GCTGCCCTGG ACTCTTGCCA GGCACAAGCC CGGCAGCTGG AGGAGGCTCT GAGGAGGCGA 
GAGGACGAGA TCCAGGACCG GGACCTCCGG CACCAGGAGG CCGTGCAGCA GCTCCAGCGG 
GCACTTGCCC AGAGAGATGA AGAATTGAGC CATCAGAAGA GACAGGGGCA GCTGCTAGAG 
CAGTCTCTGG CCCGGAGGGC CCGAAAAGAT GCCATCCAAG GGAAGCCAGG TCCGGAGCAG 
GAGAAAGAGG AAGAGATGAG AGGCCTTCGA GAAAGCCTAA GGGAGTTGCA GCTGACTCTA 
GCCCAAAAGG AAGAGGAGAT CCTGGGGCTG AGGGAGGCCC AGCAAAGGAA GAATCTGGAG 
GACTCACTGC ACAGCCACAC AGCGCCCCCA GAGCCCTCTA CAGACTTTGC CACCTTAGGG 
CCCAGGCTGC AGCAGGAGCT GGAGCGACTG CAGACAGCAC TGAGGCTAAC TGAGGCCAGG 
GAGATTGAGT GGAGGGAGAA GGCCCAGGAC TTGGCACTGT CCCTGGCCCA GAGCAAGGCT 
AGCGTCAGCA GTTTGCAGGA GGCAGCCATG TTCCTACAGG CCTCTGTTCT GGAGCGGGAC 
TTGGAACAGC AGAGGCTGCA GGATGAGTTG GAGCTCACCA GACAGGCTCT GGAGAAGGAG 
CAGCTCCTGA GCTCTAGTTC AACCAGCAGA GCAGAACAGA GGCCCAGAGA AGAGGTGTCA 
GAAGTCGAGG CTGAGCCTAG TCTTGGGCTG GAGGAGAGGC AGCTCTGGGG ACAAAGGCTG 
GAGTACCTCC AGCAAGCAGT GGCACAGCTG GAGATTGACC GGAGCAGGCT GCAGCACCAC 
AATGTCCAGC TGCGGGCCAC CTTGGAGCAG GTGGAGCGAG AGCGCAGGAA GCTGAAGAGG 
GAGTCCATGC GCGTGTCACG GACAGGCGGC CTGGAGGTTA AGGAAGCTGC GGCTTCGTCC 
CCCACACAGC AGGATGGAAG GGGAGGACAG AAGGGCTCCT CAGATGACAA GCAGATGGCT 
GAACTGCAGA AAGAGGTGGC CATGCTGCGG GGCCAGCTGG CCTTGGAGCG GAAGCAGAGG 
CAGGACTACA TTGCTCGCTC GGTGCAGACT AGCCGTGAGC TAGCGGGCCT GCACCACAGC 
CTCTCCCACT CACTGCTGGC CGTGGCCCAG GCCCCTGAAG CCACTGTCCT GGAGGCCGAG 
ACCCGCAAGC TGGACGAGTC CCTGACTCAA AGTCTGACAT CCCCAGGGCC AGCTTTGCTG 
TGCCCCAGCC CCAGCACTAT CCAGGCCATC TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962515.1
CDS	198..7496
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107040.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107040.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAGTATGACC 
CAAGGCTGTG GTCGAGACAG CTCCTTAGAA TTGGACCCTA GTGGCCTCTC ATCCCAGTTT 
GATTCCCAGG ACCCAGACAA GGCCCTTACT CTGGTGCGTT CAGTGGTGAC TCAAAGACGC 
CAGGCTGTGC AGGACCTAAG GCAGCAGCTT TCGGCCTGTC AGGAAGCTAT GAGCTCTTTG 
CGGCAGCAGC ATAATCAGTG GGAGGAGGAG GGTGAGGCCT TAAGACAGCG TCTGCAGAAG 
CTCACCGGGG AACGCGACAC TCTGGCAGGG CAGACCTCGG ACCTACAGGG AGAGGTGGAG 
TCTCTCAGCA AGGAGCGAGA GCTCCTGCAG AAGACCAGGG AGGAGCTGCA GCAGCAGTTG 
GAGGTGCTGG AGCAGGAGGC ATGGCGGCTG CGAAGGACAA ACATGGAGCT TCAGTTGCAG 
GGGGATTCTG TTCAGGGTGA GAAGGAGGAG CAGCAGGAGG AGCTGCACCT GGCTGTCCGT 
GAAAGGGAGC GCCTTCAGGA GACACTAGCA AGTCTGGAAG CCAAACAGTC AGAATCACTC 
AGTGAATTGA TCATTCTTCG GGAAGCCCTG GAGTCTTGTC ACCTGGAAGG GGAGCTGCTG 
AGGCAAGAGC AAACAGAAGT GACTGCGGCG CTGGCCAGGG CAGAACAGTC AGTTGCAGAG 
CTGTCAAGTT CTGAAAACAG CCTGAAGGCC GAGGTTGCTG ATCTTCGGGC TGCAACCATC 
AAGCTCAGCG CCTTAAATGA GGCTTTGGCC TTGGATAAGG TTGGACTGAA CCAGCAGCTT 
CTCCAGTTAG AACAAGAGAA CCAATCTGTG TGCCACAGAA TGGAAGCAAC AGAGCAGGCA 
AGAAACGCTT TGCAGTTGGG CCTGGCAGAG GCCGAGAGGA GCAGGGAAGC CCTACAGGAA 
AAGAACACTC ACCTGGAGGC ACAGCTGCAG AAGGCAGAGG AGAGGGGGGC TGAGCTGCAG 
GCAGATCTCA GGGCCATCCA AGATGAGAAG GAAGAAATTC AAGAGAAACT AAGTGAGGCA 
CGTCATCAGC AGGAGGCAGC CTTAGCTCAG CTGGATCAGC TGCGTCAGGA GACAAAGCGA 
CAGGAAGAAG TGCTTGCTCG AGAAGTCCAG GAGAAGGAGG CCTTAGTACG GGAGAGAGCA 
GCCCTAGAGG TGCGGCTGCA GGCCGTGGAG CGAGACCGGC AGGACCTCGC TGAACAACTA 
CTGGGCCTCA GCTCAGCCAA GGAGCAACTG GAGAGCACTC TGTTTGAGGC CCAACAACAA 
AATTCTCTGG TAGAGGTCAC GAAGGGACAG CTAGAGGTCC AGATTCAAAC TGTCATTCGA 
GCCAAGGAAG TAATTCAAGG GGAAGTGAGG TGCCTGAAGC TGGAACTGGA CAGTGAGCGG 
AACCAGGCAG AACAAGAGCG GGAGACAGCA GCCAGACGGC TGGCCCAGGC CGAGCAAGAG 
GGGCAGACTG CCCTGCAGCA GCAGAAGTCA GCCCACGAGG AGGAGGTGAA CCGGCTCCAG 
GAGAAATGGG AGAAGGAGCG CTCTTGGCAC CAGCAGGAAC TGGATAAGGC CCTGGAGAGC 
CTAGAGAAGG AGAAAATGGA GCTGGAAACG AGGCTGAGGG AACAGCAGGC AGAAGCCGAG 
GCCATCCGGA CACAGAGGGA GGAAGAACGG GCGGAGGCAG AGAGTGCCCT CTGCCAGATG 
CAGCTCGAAA CAGAGAAGGA GAGAGTGTCC CTCCTGGAGA CCCTGCTGCA GACCCAGAAG 
GAGCTGGCAG ATGCCAGCCA ACAACTAGAG CGGCTGAGGC AGGACATGAA GGTCCAGAAG 
TTAAAGGAGC AGGAGACCAC TCAGATCCTG CAGGCCCAGC TCCGGGAGGC TCGGGGGGAG 
CTGGAGCAGG CAGCCCAGCA GAACAGAGAT GACCTTGTTG CTGTCCAAGA AGAGTGCGGG 
GCCCTGCTGC AGGCGAAGAT GGACCTGCAG AAGCGGGTAG AAGAGTTGAA GTCTCAGCTG 
GTTTCCAGAG ATGACTCCCG GAGGCTGGTG GAGCAGGAGG TTCAGGAGAA GCTGAGGGAG 
GCCCAGGAGT ATAGCCGAAT TCAGAAGGAG CTGGAGAGAG AGAAAGCCAG CCTGATTCAG 
TCGCTGATGG AAAAGGAGCA GAGACTCCTT GTTTTACAAG AAGCTGACTC TGTTCGACAA 
CAGGAGCTGA GCTCCCTGCG CCAGGACATG CAGGAGTCCC AGGAAGGGCA GAAAGAGCTC 
AGCACCCAGG TGGAATTACT GAAGCAGGAG GTGAAGGAAA AGGAGGCTGA CTTTCTGGCT 
CAGGAAGCAC AACTGCTGGA GGAGCTGGAG ACATCTCAAG TAACAGAGCA GCGGCTGCGA 
GCTTCTTTGC GGGCCCTGGA AGCCAAGGCA GCCCAAGTCC AGCTGCGACT GCGCAGCACA 
GAGAACCAGT TGGCAGCTCT GGTGGCAGAG CAGCAGCCGG GGCACCAGGC CCAGGCCCAG 
CTGGCCAGCC TCTGTTCTGT CCTGCAGCAG GCCTTGGGGT TTGCTTGTGA GAGCAGGCCT 
GAGCTGCGTG GCGGGGGAGA CTCTGCTTCC CTCTGGGGCC CCGAGCCAGA CCAGAATGGA 
ACTGGGATCC TCCTTAAGAG AGGGCCCCTC CTGACAGCTC TGTCAGCTGA GGCAGTGGCA 
TCTGCCCTCC AGAAACTTCA CCAAGACCTA TGGAAGACTC AGCAGGCCCG GGATGATCTG 
CGGGAACAGG CCCTGAAGCT GGAACAGCGT CTCACTGATA CAGAGGCCGA GAAGAGTCAG 
GTCTGCACAG AATTGCAGGA TTTGCAGAGA CAACTCTCCC AGAACCAGGA AGAGAAATCC 
AAGTGGGAAG GAAAACAGAT CTCCCTGGAA TCTGAGCTGA CTGAACTGCA TGGAACTGTG 
GCATCATTAC AGAGTCGTCT ACAGCAAGCA GAGCTGCAGG GACTAGAGGC CCAGAATGAG 
CGAAAGCTAC TGCAGGCAGC CAAGGAGAGC CTGACAGCCC AGGTGGAACG TTTGCAGGCA 
TCTGTGGCAG AAGCCAGGGC TCAGGCTGGT GCCACCAGGG CTCTGGAGGA GGACTTGAGA 
ACTGCTCGCT CAGCCCTGAA ACTCAAGAGT GAGGAAGCAG AGACCGAGCG TGAGCGGGCC 
CAGGCTCTGC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAGGCTCT GCAGGAGAAT 
TTAGCTATCC TGGTTCAGAC TCTATCCGAA AGAGAAGGGG AGGTGGAGGC TTTGCGGGGA 
AGTATTCAGG AACTGGAGAA ACAACGGGAG ATGCAAAAGG CTACTTTGGA AGCTCTGTCT 
CTGGACCTGA AGAAGAAGAG CGAAGAGGTA GATGTGCAAC AAGAACAGAT CCAGGAGCTG 
GAGAAGTGCA GGTCCCTTTT AGAAGATCTG CCTCTGGCCA TGCAGGAACA AGAGCAGAGG 
CTGGTTGCAC AGAGGGAGCA AATCCAAGAG CTCGAGAAGG ATCGAGAGAC CCAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT CGAACTTGAG AAGAAGGCCC AAATGATAGA GTCCCAGAAA 
GGAGAGATTC AGGACCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC TCTGGAACGA 
GAGGAAAACC ATCACAAGAT GGAGTGCCAA CAGAAGGCAA TCGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGAGTAGC TCTGACCCAC CTCACACTGG ACCTGGAAGA AAAGAGCCAG 
GAGCTGCAGG CCCAGAGCAG TCAGATCGAC AAGCTGGAGA GCCATAGCAC CCTTCTGGCG 
AAAGAGCTCC AGGACAAGGA CCAGGAGCTG AAGTCCCAGC AAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AGGAGCGTCT GGCTCAGGAC CTAGAGAGGA GGGACCAGGA CGTGGTGCTC 
CAGAGGGAAA GGATTCGGGT CCTAGAAGAC CAGAGGACAC TGCAGACCAA GATCCTGCAG 
GAGGACCTGG GACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GGCTTCCCAG 
AGGCCACCGA TGCCGGAGTG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC CCAGCGCGGG 
AGCCTGGAGC ACCTGAAGCT GATCCTGCGT GACAAGGAGA GGGAGGTAGA ATGCCAGCAG 
GAACGCATCC AGGAACTGAA GGAGTATAAG GATCAGCTGG AGCAGCAGCT CCAGGGCCTA 
CACAGGAAGG CAGGGGAGAC TGGCCTCCTC CTGACTCAGC GAGAGCAGGA GATAGTGGTC 
CTGCAGCGGC ATCTGCAGGA AGCCGCAGAA CAGGGGGAGC TGAAAGAGCG GTCACTTCAG 
GGTCACCTGG AAGAGGCCCA GAGAGCCCTG GCCCAGAGGG ACCAGGAGCT CGAGGCCCTG 
CAGCACCAAC AGCAGCAGGC CCTGGGGCAG GAGGAGACTA GGAAGGAAGA GGCAAGCACC 
CTGCAGAGGG CTCTGGAGCA GGCCCACACG GCGCTGAAAG AGCGCCAGGG AGAGCTTGAG 
GACCACAAGG AGCATGTGCG AAGGCTCCAG GAGGAGCTGG CCGTGGAGGG ACGGCGTGTG 
CAGGCCCTGG AGGAGGTGGT GGGTGACCTA AGGGCTGAGT CTCGGGAGCA GGAGGAGGCT 
TTGCTGGCCC TCCAGCAACA GGGTGCCGAG CGGGCACAGG AGCATGAGGC GGAGGTCAGG 
GGCCTGCGGG CCAGCCTCCT GCAGGCAGAG ACTGCACTCA AGGAACGGGA CCTGGCGCTG 
GAGGCCCTGC GAGCCGATGG CCGGGCCTCC CAGCTTCGGG AGGAGACAGC CCGGGACTGG 
GCCCAAGCTC TGCAGGAGGC CCTGAGCAAG GCCCAGGCTG CCGTGCAGGA GAAAGAGCAG 
CGGCTGCTGG GTCAAGCAGA GTTGAGCCGC AGCCTGGAGA CCAGCACCGC CACTTTGCAG 
GCTGCCCTGG ACTCTTGCCA GGCACAAGCC CGGCAGCTGG AGGAGGCTCT GAGGAGGCGA 
GAGGACGAGA TCCAGGACCG GGACCTCCGG CACCAGGAGG CCGTGCAGCA GCTCCAGCGG 
GCACTTGCCC AGAGAGATGA AGAATTGAGC CATCAGAAGA GACAGGGGCA GCTGCTAGAG 
CAGTCTCTGG CCCGGAGGGC CCGAAAAGAT GCCATCCAAG GGAAGCCAGG TCCGGAGCAG 
GAGAAAGAGG AAGAGATGAG AGGCCTTCGA GAAAGCCTAA GGGAGTTGCA GCTGACTCTA 
GCCCAAAAGG AAGAGGAGAT CCTGGGGCTG AGGGAGGCCC AGCAAAGGAA GAATCTGGAG 
GACTCACTGC ACAGCCACAC AGCGCCCCCA GAGCCCTCTA CAGACTTTGC CACCTTAGGG 
CCCAGGCTGC AGCAGGAGCT GGAGCGACTG CAGACAGCAC TGAGGCTAAC TGAGGCCAGG 
GAGATTGAGT GGAGGGAGAA GGCCCAGGAC TTGGCACTGT CCCTGGCCCA GAGCAAGGCT 
AGCGTCAGCA GTTTGCAGGA GGCAGCCATG TTCCTACAGG CCTCTGTTCT GGAGCGGGAC 
TTGGAACAGC AGAGGCTGCA GGATGAGTTG GAGCTCACCA GACAGGCTCT GGAGAAGGAG 
CAGCTCCTGA GCTCTAGTTC AACCAGCAGA GCAGAACAGA GGCCCAGAGA AGAGGTGTCA 
GAAGTCGAGG CTGAGCCTAG TCTTGGGCTG GAGGAGAGGC AGCTCTGGGG ACAAAGGCTG 
GAGTACCTCC AGCAAGCAGT GGCACAGCTG GAGATTGACC GGAGCAGGCT GCAGCACCAC 
AATGTCCAGC TGCGGGCCAC CTTGGAGCAG GTGGAGCGAG AGCGCAGGAA GCTGAAGAGG 
GAGTCCATGC GCGTGTCACG GACAGGCGGC CTGGAGGTTA AGGAAGCTGC GGCTTCGTCC 
CCCACACAGC AGGATGGAAG GGGAGGACAG AAGGGCTCCT CAGATGACAA GCAGATGGCT 
GAACTGCAGA AAGAGGTGGC CATGCTGCGG GGCCAGCTGG CCTTGGAGCG GAAGCAGAGG 
CAGGACTACA TTGCTCGCTC GGTGCAGACT AGCCGTGAGC TAGCGGGCCT GCACCACAGC 
CTCTCCCACT CACTGCTGGC CGTGGCCCAG GCCCCTGAAG CCACTGTCCT GGAGGCCGAG 
ACCCGCAAGC TGGACGAGTC CCTGACTCAA AGTCTGACAT CCCCAGGGCC AGCTTTGCTG 
TGCCCCAGCC CCAGCACTAT CCAGGCCATC TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107041.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962516.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

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CloneID	OCe42763
Clone ID Related Accession (Same CDS sequence)	XM_014107041.1 , XM_014107041.2
Accession Version	XM_014107041.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGTGTCAGA AGTCGAGGCT 
GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG CTCTGGGGAC AAAGGCTGGA GTACCTCCAG 
CAAGCAGTGG CACAGCTGGA GATTGACCGG AGCAGGCTGC AGCACCACAA TGTCCAGCTG 
CGGGCCACCT TGGAGCAGGT GGAGCGAGAG CGCAGGAAGC TGAAGAGGGA GTCCATGCGC 
GTGTCACGGA CAGGCGGCCT GGAGGTTAAG GAAGCTGCGG CTTCGTCCCC CACACAGCAG 
GATGGAAGGG GAGGACAGAA GGGCTCCTCA GATGACAAGC AGATGGCTGA ACTGCAGAAA 
GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC TTGGAGCGGA AGCAGAGGCA GGACTACATT 
GCTCGCTCGG TGCAGACTAG CCGTGAGCTA GCGGGCCTGC ACCACAGCCT CTCCCACTCA 
CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GACGAGTCCC TGACTCAAAG TCTGACATCC CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC 
AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962516.1
CDS	198..7424
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107041.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107041.1

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGTGTCAGA AGTCGAGGCT 
GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG CTCTGGGGAC AAAGGCTGGA GTACCTCCAG 
CAAGCAGTGG CACAGCTGGA GATTGACCGG AGCAGGCTGC AGCACCACAA TGTCCAGCTG 
CGGGCCACCT TGGAGCAGGT GGAGCGAGAG CGCAGGAAGC TGAAGAGGGA GTCCATGCGC 
GTGTCACGGA CAGGCGGCCT GGAGGTTAAG GAAGCTGCGG CTTCGTCCCC CACACAGCAG 
GATGGAAGGG GAGGACAGAA GGGCTCCTCA GATGACAAGC AGATGGCTGA ACTGCAGAAA 
GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC TTGGAGCGGA AGCAGAGGCA GGACTACATT 
GCTCGCTCGG TGCAGACTAG CCGTGAGCTA GCGGGCCTGC ACCACAGCCT CTCCCACTCA 
CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GACGAGTCCC TGACTCAAAG TCTGACATCC CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC 
AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_014107041.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962516.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

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CloneID	OCe42763
Clone ID Related Accession (Same CDS sequence)	XM_014107041.1 , XM_014107041.2
Accession Version	XM_014107041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014107041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGTGTCAGA AGTCGAGGCT 
GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG CTCTGGGGAC AAAGGCTGGA GTACCTCCAG 
CAAGCAGTGG CACAGCTGGA GATTGACCGG AGCAGGCTGC AGCACCACAA TGTCCAGCTG 
CGGGCCACCT TGGAGCAGGT GGAGCGAGAG CGCAGGAAGC TGAAGAGGGA GTCCATGCGC 
GTGTCACGGA CAGGCGGCCT GGAGGTTAAG GAAGCTGCGG CTTCGTCCCC CACACAGCAG 
GATGGAAGGG GAGGACAGAA GGGCTCCTCA GATGACAAGC AGATGGCTGA ACTGCAGAAA 
GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC TTGGAGCGGA AGCAGAGGCA GGACTACATT 
GCTCGCTCGG TGCAGACTAG CCGTGAGCTA GCGGGCCTGC ACCACAGCCT CTCCCACTCA 
CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GACGAGTCCC TGACTCAAAG TCTGACATCC CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC 
AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962516.1
CDS	212..7438
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEISSSQARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_014107041.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107041.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGATTTCTT CCTCTCAGGC ACGTCATCAG CAGGAGGCAG CCTTAGCTCA GCTGGATCAG 
CTGCGTCAGG AGACAAAGCG ACAGGAAGAA GTGCTTGCTC GAGAAGTCCA GGAGAAGGAG 
GCCTTAGTAC GGGAGAGAGC AGCCCTAGAG GTGCGGCTGC AGGCCGTGGA GCGAGACCGG 
CAGGACCTCG CTGAACAACT ACTGGGCCTC AGCTCAGCCA AGGAGCAACT GGAGAGCACT 
CTGTTTGAGG CCCAACAACA AAATTCTCTG GTAGAGGTCA CGAAGGGACA GCTAGAGGTC 
CAGATTCAAA CTGTCATTCG AGCCAAGGAA GTAATTCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACAGTGAGCG GAACCAGGCA GAACAAGAGC GGGAGACAGC AGCCAGACGG 
CTGGCCCAGG CCGAGCAAGA GGGGCAGACT GCCCTGCAGC AGCAGAAGTC AGCCCACGAG 
GAGGAGGTGA ACCGGCTCCA GGAGAAATGG GAGAAGGAGC GCTCTTGGCA CCAGCAGGAA 
CTGGATAAGG CCCTGGAGAG CCTAGAGAAG GAGAAAATGG AGCTGGAAAC GAGGCTGAGG 
GAACAGCAGG CAGAAGCCGA GGCCATCCGG ACACAGAGGG AGGAAGAACG GGCGGAGGCA 
GAGAGTGCCC TCTGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAGTGTC CCTCCTGGAG 
ACCCTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTAGA GCGGCTGAGG 
CAGGACATGA AGGTCCAGAA GTTAAAGGAG CAGGAGACCA CTCAGATCCT GCAGGCCCAG 
CTCCGGGAGG CTCGGGGGGA GCTGGAGCAG GCAGCCCAGC AGAACAGAGA TGACCTTGTT 
GCTGTCCAAG AAGAGTGCGG GGCCCTGCTG CAGGCGAAGA TGGACCTGCA GAAGCGGGTA 
GAAGAGTTGA AGTCTCAGCT GGTTTCCAGA GATGACTCCC GGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGGGA GGCCCAGGAG TATAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGATTCA GTCGCTGATG GAAAAGGAGC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTGTTCGACA ACAGGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGTCC 
CAGGAAGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC TCAGGAAGCA CAACTGCTGG AGGAGCTGGA GACATCTCAA 
GTAACAGAGC AGCGGCTGCG AGCTTCTTTG CGGGCCCTGG AAGCCAAGGC AGCCCAAGTC 
CAGCTGCGAC TGCGCAGCAC AGAGAACCAG TTGGCAGCTC TGGTGGCAGA GCAGCAGCCG 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTCTGTTCTG TCCTGCAGCA GGCCTTGGGG 
TTTGCTTGTG AGAGCAGGCC TGAGCTGCGT GGCGGGGGAG ACTCTGCTTC CCTCTGGGGC 
CCCGAGCCAG ACCAGAATGG AACTGGGATC CTCCTTAAGA GAGGGCCCCT CCTGACAGCT 
CTGTCAGCTG AGGCAGTGGC ATCTGCCCTC CAGAAACTTC ACCAAGACCT ATGGAAGACT 
CAGCAGGCCC GGGATGATCT GCGGGAACAG GCCCTGAAGC TGGAACAGCG TCTCACTGAT 
ACAGAGGCCG AGAAGAGTCA GGTCTGCACA GAATTGCAGG ATTTGCAGAG ACAACTCTCC 
CAGAACCAGG AAGAGAAATC CAAGTGGGAA GGAAAACAGA TCTCCCTGGA ATCTGAGCTG 
ACTGAACTGC ATGGAACTGT GGCATCATTA CAGAGTCGTC TACAGCAAGC AGAGCTGCAG 
GGACTAGAGG CCCAGAATGA GCGAAAGCTA CTGCAGGCAG CCAAGGAGAG CCTGACAGCC 
CAGGTGGAAC GTTTGCAGGC ATCTGTGGCA GAAGCCAGGG CTCAGGCTGG TGCCACCAGG 
GCTCTGGAGG AGGACTTGAG AACTGCTCGC TCAGCCCTGA AACTCAAGAG TGAGGAAGCA 
GAGACCGAGC GTGAGCGGGC CCAGGCTCTG CAGGAGCAGG GCGAGCTGAA GGTGGCCCAA 
GGGAAGGCTC TGCAGGAGAA TTTAGCTATC CTGGTTCAGA CTCTATCCGA AAGAGAAGGG 
GAGGTGGAGG CTTTGCGGGG AAGTATTCAG GAACTGGAGA AACAACGGGA GATGCAAAAG 
GCTACTTTGG AAGCTCTGTC TCTGGACCTG AAGAAGAAGA GCGAAGAGGT AGATGTGCAA 
CAAGAACAGA TCCAGGAGCT GGAGAAGTGC AGGTCCCTTT TAGAAGATCT GCCTCTGGCC 
ATGCAGGAAC AAGAGCAGAG GCTGGTTGCA CAGAGGGAGC AAATCCAAGA GCTCGAGAAG 
GATCGAGAGA CCCAGAGGAA CATCTTGGAG CATCAGCTTC TCGAACTTGA GAAGAAGGCC 
CAAATGATAG AGTCCCAGAA AGGAGAGATT CAGGACCTGA AGAAGCAGCT GGTTACTCTG 
GAATGCCTGG CTCTGGAACG AGAGGAAAAC CATCACAAGA TGGAGTGCCA ACAGAAGGCA 
ATCGAGGAGC TGGAGGGCCA GAGGGAAATG CAGAGAGTAG CTCTGACCCA CCTCACACTG 
GACCTGGAAG AAAAGAGCCA GGAGCTGCAG GCCCAGAGCA GTCAGATCGA CAAGCTGGAG 
AGCCATAGCA CCCTTCTGGC GAAAGAGCTC CAGGACAAGG ACCAGGAGCT GAAGTCCCAG 
CAAGAACAGA TCGAGGAGCT GCAGAGGCAG AAGGAGCGTC TGGCTCAGGA CCTAGAGAGG 
AGGGACCAGG ACGTGGTGCT CCAGAGGGAA AGGATTCGGG TCCTAGAAGA CCAGAGGACA 
CTGCAGACCA AGATCCTGCA GGAGGACCTG GGACAGATCA AGCTGTCCTT GAGAGAGCGA 
GGCCGGGAGC TGGCTTCCCA GAGGCCACCG ATGCCGGAGT GGGCAGAGGA AGGGAAGGGC 
CAGAGTAAAG CCCAGCGCGG GAGCCTGGAG CACCTGAAGC TGATCCTGCG TGACAAGGAG 
AGGGAGGTAG AATGCCAGCA GGAACGCATC CAGGAACTGA AGGAGTATAA GGATCAGCTG 
GAGCAGCAGC TCCAGGGCCT ACACAGGAAG GCAGGGGAGA CTGGCCTCCT CCTGACTCAG 
CGAGAGCAGG AGATAGTGGT CCTGCAGCGG CATCTGCAGG AAGCCGCAGA ACAGGGGGAG 
CTGAAAGAGC GGTCACTTCA GGGTCACCTG GAAGAGGCCC AGAGAGCCCT GGCCCAGAGG 
GACCAGGAGC TCGAGGCCCT GCAGCACCAA CAGCAGCAGG CCCTGGGGCA GGAGGAGACT 
AGGAAGGAAG AGGCAAGCAC CCTGCAGAGG GCTCTGGAGC AGGCCCACAC GGCGCTGAAA 
GAGCGCCAGG GAGAGCTTGA GGACCACAAG GAGCATGTGC GAAGGCTCCA GGAGGAGCTG 
GCCGTGGAGG GACGGCGTGT GCAGGCCCTG GAGGAGGTGG TGGGTGACCT AAGGGCTGAG 
TCTCGGGAGC AGGAGGAGGC TTTGCTGGCC CTCCAGCAAC AGGGTGCCGA GCGGGCACAG 
GAGCATGAGG CGGAGGTCAG GGGCCTGCGG GCCAGCCTCC TGCAGGCAGA GACTGCACTC 
AAGGAACGGG ACCTGGCGCT GGAGGCCCTG CGAGCCGATG GCCGGGCCTC CCAGCTTCGG 
GAGGAGACAG CCCGGGACTG GGCCCAAGCT CTGCAGGAGG CCCTGAGCAA GGCCCAGGCT 
GCCGTGCAGG AGAAAGAGCA GCGGCTGCTG GGTCAAGCAG AGTTGAGCCG CAGCCTGGAG 
ACCAGCACCG CCACTTTGCA GGCTGCCCTG GACTCTTGCC AGGCACAAGC CCGGCAGCTG 
GAGGAGGCTC TGAGGAGGCG AGAGGACGAG ATCCAGGACC GGGACCTCCG GCACCAGGAG 
GCCGTGCAGC AGCTCCAGCG GGCACTTGCC CAGAGAGATG AAGAATTGAG CCATCAGAAG 
AGACAGGGGC AGCTGCTAGA GCAGTCTCTG GCCCGGAGGG CCCGAAAAGA TGCCATCCAA 
GGGAAGCCAG GTCCGGAGCA GGAGAAAGAG GAAGAGATGA GAGGCCTTCG AGAAAGCCTA 
AGGGAGTTGC AGCTGACTCT AGCCCAAAAG GAAGAGGAGA TCCTGGGGCT GAGGGAGGCC 
CAGCAAAGGA AGAATCTGGA GGACTCACTG CACAGCCACA CAGCGCCCCC AGAGCCCTCT 
ACAGACTTTG CCACCTTAGG GCCCAGGCTG CAGCAGGAGC TGGAGCGACT GCAGACAGCA 
CTGAGGCTAA CTGAGGCCAG GGAGATTGAG TGGAGGGAGA AGGCCCAGGA CTTGGCACTG 
TCCCTGGCCC AGAGCAAGGC TAGCGTCAGC AGTTTGCAGG AGGCAGCCAT GTTCCTACAG 
GCCTCTGTTC TGGAGCGGGA CTTGGAACAG CAGAGGCTGC AGGTGTCAGA AGTCGAGGCT 
GAGCCTAGTC TTGGGCTGGA GGAGAGGCAG CTCTGGGGAC AAAGGCTGGA GTACCTCCAG 
CAAGCAGTGG CACAGCTGGA GATTGACCGG AGCAGGCTGC AGCACCACAA TGTCCAGCTG 
CGGGCCACCT TGGAGCAGGT GGAGCGAGAG CGCAGGAAGC TGAAGAGGGA GTCCATGCGC 
GTGTCACGGA CAGGCGGCCT GGAGGTTAAG GAAGCTGCGG CTTCGTCCCC CACACAGCAG 
GATGGAAGGG GAGGACAGAA GGGCTCCTCA GATGACAAGC AGATGGCTGA ACTGCAGAAA 
GAGGTGGCCA TGCTGCGGGG CCAGCTGGCC TTGGAGCGGA AGCAGAGGCA GGACTACATT 
GCTCGCTCGG TGCAGACTAG CCGTGAGCTA GCGGGCCTGC ACCACAGCCT CTCCCACTCA 
CTGCTGGCCG TGGCCCAGGC CCCTGAAGCC ACTGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GACGAGTCCC TGACTCAAAG TCTGACATCC CCAGGGCCAG CTTTGCTGTG CCCCAGCCCC 
AGCACTATCC AGGCCATCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005634972.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635029.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

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CloneID	OCe24790
Clone ID Related Accession (Same CDS sequence)	XM_005634972.2 , XM_005634972.3
Accession Version	XM_005634972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635029.1
CDS	198..7502
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_005634972.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634972.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005634972.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635029.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OCe24790
Clone ID Related Accession (Same CDS sequence)	XM_005634972.2 , XM_005634972.3
Accession Version	XM_005634972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635029.1
CDS	212..7516
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEARHQQEAALAQLDQLRQETKRQEEVLAREVQEKEALVRERAALEVRLQAVERDRQDLAEQLLGLSSAKEQLESTLFEAQQQNSLVEVTKGQLEVQIQTVIRAKEVIQGEVRCLKLELDSERNQAEQERETAARRLAQAEQEGQTALQQQKSAHEEEVNRLQEKWEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_005634972.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634972.3

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGCACGTC ATCAGCAGGA GGCAGCCTTA GCTCAGCTGG ATCAGCTGCG TCAGGAGACA 
AAGCGACAGG AAGAAGTGCT TGCTCGAGAA GTCCAGGAGA AGGAGGCCTT AGTACGGGAG 
AGAGCAGCCC TAGAGGTGCG GCTGCAGGCC GTGGAGCGAG ACCGGCAGGA CCTCGCTGAA 
CAACTACTGG GCCTCAGCTC AGCCAAGGAG CAACTGGAGA GCACTCTGTT TGAGGCCCAA 
CAACAAAATT CTCTGGTAGA GGTCACGAAG GGACAGCTAG AGGTCCAGAT TCAAACTGTC 
ATTCGAGCCA AGGAAGTAAT TCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACAGT 
GAGCGGAACC AGGCAGAACA AGAGCGGGAG ACAGCAGCCA GACGGCTGGC CCAGGCCGAG 
CAAGAGGGGC AGACTGCCCT GCAGCAGCAG AAGTCAGCCC ACGAGGAGGA GGTGAACCGG 
CTCCAGGAGA AATGGGAGAA GGAGCGCTCT TGGCACCAGC AGGAACTGGA TAAGGCCCTG 
GAGAGCCTAG AGAAGGAGAA AATGGAGCTG GAAACGAGGC TGAGGGAACA GCAGGCAGAA 
GCCGAGGCCA TCCGGACACA GAGGGAGGAA GAACGGGCGG AGGCAGAGAG TGCCCTCTGC 
CAGATGCAGC TCGAAACAGA GAAGGAGAGA GTGTCCCTCC TGGAGACCCT GCTGCAGACC 
CAGAAGGAGC TGGCAGATGC CAGCCAACAA CTAGAGCGGC TGAGGCAGGA CATGAAGGTC 
CAGAAGTTAA AGGAGCAGGA GACCACTCAG ATCCTGCAGG CCCAGCTCCG GGAGGCTCGG 
GGGGAGCTGG AGCAGGCAGC CCAGCAGAAC AGAGATGACC TTGTTGCTGT CCAAGAAGAG 
TGCGGGGCCC TGCTGCAGGC GAAGATGGAC CTGCAGAAGC GGGTAGAAGA GTTGAAGTCT 
CAGCTGGTTT CCAGAGATGA CTCCCGGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGGGAGGCCC AGGAGTATAG CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ATTCAGTCGC TGATGGAAAA GGAGCAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTGTT 
CGACAACAGG AGCTGAGCTC CCTGCGCCAG GACATGCAGG AGTCCCAGGA AGGGCAGAAA 
GAGCTCAGCA CCCAGGTGGA ATTACTGAAG CAGGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCTCAGG AAGCACAACT GCTGGAGGAG CTGGAGACAT CTCAAGTAAC AGAGCAGCGG 
CTGCGAGCTT CTTTGCGGGC CCTGGAAGCC AAGGCAGCCC AAGTCCAGCT GCGACTGCGC 
AGCACAGAGA ACCAGTTGGC AGCTCTGGTG GCAGAGCAGC AGCCGGGGCA CCAGGCCCAG 
GCCCAGCTGG CCAGCCTCTG TTCTGTCCTG CAGCAGGCCT TGGGGTTTGC TTGTGAGAGC 
AGGCCTGAGC TGCGTGGCGG GGGAGACTCT GCTTCCCTCT GGGGCCCCGA GCCAGACCAG 
AATGGAACTG GGATCCTCCT TAAGAGAGGG CCCCTCCTGA CAGCTCTGTC AGCTGAGGCA 
GTGGCATCTG CCCTCCAGAA ACTTCACCAA GACCTATGGA AGACTCAGCA GGCCCGGGAT 
GATCTGCGGG AACAGGCCCT GAAGCTGGAA CAGCGTCTCA CTGATACAGA GGCCGAGAAG 
AGTCAGGTCT GCACAGAATT GCAGGATTTG CAGAGACAAC TCTCCCAGAA CCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA ACAGATCTCC CTGGAATCTG AGCTGACTGA ACTGCATGGA 
ACTGTGGCAT CATTACAGAG TCGTCTACAG CAAGCAGAGC TGCAGGGACT AGAGGCCCAG 
AATGAGCGAA AGCTACTGCA GGCAGCCAAG GAGAGCCTGA CAGCCCAGGT GGAACGTTTG 
CAGGCATCTG TGGCAGAAGC CAGGGCTCAG GCTGGTGCCA CCAGGGCTCT GGAGGAGGAC 
TTGAGAACTG CTCGCTCAGC CCTGAAACTC AAGAGTGAGG AAGCAGAGAC CGAGCGTGAG 
CGGGCCCAGG CTCTGCAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA GGCTCTGCAG 
GAGAATTTAG CTATCCTGGT TCAGACTCTA TCCGAAAGAG AAGGGGAGGT GGAGGCTTTG 
CGGGGAAGTA TTCAGGAACT GGAGAAACAA CGGGAGATGC AAAAGGCTAC TTTGGAAGCT 
CTGTCTCTGG ACCTGAAGAA GAAGAGCGAA GAGGTAGATG TGCAACAAGA ACAGATCCAG 
GAGCTGGAGA AGTGCAGGTC CCTTTTAGAA GATCTGCCTC TGGCCATGCA GGAACAAGAG 
CAGAGGCTGG TTGCACAGAG GGAGCAAATC CAAGAGCTCG AGAAGGATCG AGAGACCCAG 
AGGAACATCT TGGAGCATCA GCTTCTCGAA CTTGAGAAGA AGGCCCAAAT GATAGAGTCC 
CAGAAAGGAG AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCTCTG 
GAACGAGAGG AAAACCATCA CAAGATGGAG TGCCAACAGA AGGCAATCGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG AGTAGCTCTG ACCCACCTCA CACTGGACCT GGAAGAAAAG 
AGCCAGGAGC TGCAGGCCCA GAGCAGTCAG ATCGACAAGC TGGAGAGCCA TAGCACCCTT 
CTGGCGAAAG AGCTCCAGGA CAAGGACCAG GAGCTGAAGT CCCAGCAAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAGGA GCGTCTGGCT CAGGACCTAG AGAGGAGGGA CCAGGACGTG 
GTGCTCCAGA GGGAAAGGAT TCGGGTCCTA GAAGACCAGA GGACACTGCA GACCAAGATC 
CTGCAGGAGG ACCTGGGACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGGCT 
TCCCAGAGGC CACCGATGCC GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCCCAG 
CGCGGGAGCC TGGAGCACCT GAAGCTGATC CTGCGTGACA AGGAGAGGGA GGTAGAATGC 
CAGCAGGAAC GCATCCAGGA ACTGAAGGAG TATAAGGATC AGCTGGAGCA GCAGCTCCAG 
GGCCTACACA GGAAGGCAGG GGAGACTGGC CTCCTCCTGA CTCAGCGAGA GCAGGAGATA 
GTGGTCCTGC AGCGGCATCT GCAGGAAGCC GCAGAACAGG GGGAGCTGAA AGAGCGGTCA 
CTTCAGGGTC ACCTGGAAGA GGCCCAGAGA GCCCTGGCCC AGAGGGACCA GGAGCTCGAG 
GCCCTGCAGC ACCAACAGCA GCAGGCCCTG GGGCAGGAGG AGACTAGGAA GGAAGAGGCA 
AGCACCCTGC AGAGGGCTCT GGAGCAGGCC CACACGGCGC TGAAAGAGCG CCAGGGAGAG 
CTTGAGGACC ACAAGGAGCA TGTGCGAAGG CTCCAGGAGG AGCTGGCCGT GGAGGGACGG 
CGTGTGCAGG CCCTGGAGGA GGTGGTGGGT GACCTAAGGG CTGAGTCTCG GGAGCAGGAG 
GAGGCTTTGC TGGCCCTCCA GCAACAGGGT GCCGAGCGGG CACAGGAGCA TGAGGCGGAG 
GTCAGGGGCC TGCGGGCCAG CCTCCTGCAG GCAGAGACTG CACTCAAGGA ACGGGACCTG 
GCGCTGGAGG CCCTGCGAGC CGATGGCCGG GCCTCCCAGC TTCGGGAGGA GACAGCCCGG 
GACTGGGCCC AAGCTCTGCA GGAGGCCCTG AGCAAGGCCC AGGCTGCCGT GCAGGAGAAA 
GAGCAGCGGC TGCTGGGTCA AGCAGAGTTG AGCCGCAGCC TGGAGACCAG CACCGCCACT 
TTGCAGGCTG CCCTGGACTC TTGCCAGGCA CAAGCCCGGC AGCTGGAGGA GGCTCTGAGG 
AGGCGAGAGG ACGAGATCCA GGACCGGGAC CTCCGGCACC AGGAGGCCGT GCAGCAGCTC 
CAGCGGGCAC TTGCCCAGAG AGATGAAGAA TTGAGCCATC AGAAGAGACA GGGGCAGCTG 
CTAGAGCAGT CTCTGGCCCG GAGGGCCCGA AAAGATGCCA TCCAAGGGAA GCCAGGTCCG 
GAGCAGGAGA AAGAGGAAGA GATGAGAGGC CTTCGAGAAA GCCTAAGGGA GTTGCAGCTG 
ACTCTAGCCC AAAAGGAAGA GGAGATCCTG GGGCTGAGGG AGGCCCAGCA AAGGAAGAAT 
CTGGAGGACT CACTGCACAG CCACACAGCG CCCCCAGAGC CCTCTACAGA CTTTGCCACC 
TTAGGGCCCA GGCTGCAGCA GGAGCTGGAG CGACTGCAGA CAGCACTGAG GCTAACTGAG 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTGTCCCT GGCCCAGAGC 
AAGGCTAGCG TCAGCAGTTT GCAGGAGGCA GCCATGTTCC TACAGGCCTC TGTTCTGGAG 
CGGGACTTGG AACAGCAGAG GCTGCAGGAT GAGTTGGAGC TCACCAGACA GGCTCTGGAG 
AAGGAGCAGC TCCTGAGCTC TAGTTCAACC AGCAGAGCAG AACAGAGGCC CAGAGAAGAG 
GTGTCAGAAG TCGAGGCTGA GCCTAGTCTT GGGCTGGAGG AGAGGCAGCT CTGGGGACAA 
AGGCTGGAGT ACCTCCAGCA AGCAGTGGCA CAGCTGGAGA TTGACCGGAG CAGGCTGCAG 
CACCACAATG TCCAGCTGCG GGCCACCTTG GAGCAGGTGG AGCGAGAGCG CAGGAAGCTG 
AAGAGGGAGT CCATGCGCGT GTCACGGACA GGCGGCCTGG AGGTTAAGGA AGCTGCGGCT 
TCGTCCCCCA CACAGCAGGA TGGAAGGGGA GGACAGAAGG GCTCCTCAGA TGACAAGCAG 
ATGGCTGAAC TGCAGAAAGA GGTGGCCATG CTGCGGGGCC AGCTGGCCTT GGAGCGGAAG 
CAGAGGCAGG ACTACATTGC TCGCTCGGTG CAGACTAGCC GTGAGCTAGC GGGCCTGCAC 
CACAGCCTCT CCCACTCACT GCTGGCCGTG GCCCAGGCCC CTGAAGCCAC TGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCCCTG ACTCAAAGTC TGACATCCCC AGGGCCAGCT 
TTGCTGTGCC CCAGCCCCAG CACTATCCAG GCCATCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005634976.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635033.1 Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

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CloneID	OCe24793
Clone ID Related Accession (Same CDS sequence)	XM_005634976.3 , XM_005634976.2
Accession Version	XM_005634976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	centrosome-associated protein CEP250 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005634976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGAGAAGG AGCGCTCTTG GCACCAGCAG GAACTGGATA AGGCCCTGGA GAGCCTAGAG 
AAGGAGAAAA TGGAGCTGGA AACGAGGCTG AGGGAACAGC AGGCAGAAGC CGAGGCCATC 
CGGACACAGA GGGAGGAAGA ACGGGCGGAG GCAGAGAGTG CCCTCTGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAGT GTCCCTCCTG GAGACCCTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT AGAGCGGCTG AGGCAGGACA TGAAGGTCCA GAAGTTAAAG 
GAGCAGGAGA CCACTCAGAT CCTGCAGGCC CAGCTCCGGG AGGCTCGGGG GGAGCTGGAG 
CAGGCAGCCC AGCAGAACAG AGATGACCTT GTTGCTGTCC AAGAAGAGTG CGGGGCCCTG 
CTGCAGGCGA AGATGGACCT GCAGAAGCGG GTAGAAGAGT TGAAGTCTCA GCTGGTTTCC 
AGAGATGACT CCCGGAGGCT GGTGGAGCAG GAGGTTCAGG AGAAGCTGAG GGAGGCCCAG 
GAGTATAGCC GAATTCAGAA GGAGCTGGAG AGAGAGAAAG CCAGCCTGAT TCAGTCGCTG 
ATGGAAAAGG AGCAGAGACT CCTTGTTTTA CAAGAAGCTG ACTCTGTTCG ACAACAGGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG TCCCAGGAAG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTTCT GGCTCAGGAA 
GCACAACTGC TGGAGGAGCT GGAGACATCT CAAGTAACAG AGCAGCGGCT GCGAGCTTCT 
TTGCGGGCCC TGGAAGCCAA GGCAGCCCAA GTCCAGCTGC GACTGCGCAG CACAGAGAAC 
CAGTTGGCAG CTCTGGTGGC AGAGCAGCAG CCGGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTGTT CTGTCCTGCA GCAGGCCTTG GGGTTTGCTT GTGAGAGCAG GCCTGAGCTG 
CGTGGCGGGG GAGACTCTGC TTCCCTCTGG GGCCCCGAGC CAGACCAGAA TGGAACTGGG 
ATCCTCCTTA AGAGAGGGCC CCTCCTGACA GCTCTGTCAG CTGAGGCAGT GGCATCTGCC 
CTCCAGAAAC TTCACCAAGA CCTATGGAAG ACTCAGCAGG CCCGGGATGA TCTGCGGGAA 
CAGGCCCTGA AGCTGGAACA GCGTCTCACT GATACAGAGG CCGAGAAGAG TCAGGTCTGC 
ACAGAATTGC AGGATTTGCA GAGACAACTC TCCCAGAACC AGGAAGAGAA ATCCAAGTGG 
GAAGGAAAAC AGATCTCCCT GGAATCTGAG CTGACTGAAC TGCATGGAAC TGTGGCATCA 
TTACAGAGTC GTCTACAGCA AGCAGAGCTG CAGGGACTAG AGGCCCAGAA TGAGCGAAAG 
CTACTGCAGG CAGCCAAGGA GAGCCTGACA GCCCAGGTGG AACGTTTGCA GGCATCTGTG 
GCAGAAGCCA GGGCTCAGGC TGGTGCCACC AGGGCTCTGG AGGAGGACTT GAGAACTGCT 
CGCTCAGCCC TGAAACTCAA GAGTGAGGAA GCAGAGACCG AGCGTGAGCG GGCCCAGGCT 
CTGCAGGAGC AGGGCGAGCT GAAGGTGGCC CAAGGGAAGG CTCTGCAGGA GAATTTAGCT 
ATCCTGGTTC AGACTCTATC CGAAAGAGAA GGGGAGGTGG AGGCTTTGCG GGGAAGTATT 
CAGGAACTGG AGAAACAACG GGAGATGCAA AAGGCTACTT TGGAAGCTCT GTCTCTGGAC 
CTGAAGAAGA AGAGCGAAGA GGTAGATGTG CAACAAGAAC AGATCCAGGA GCTGGAGAAG 
TGCAGGTCCC TTTTAGAAGA TCTGCCTCTG GCCATGCAGG AACAAGAGCA GAGGCTGGTT 
GCACAGAGGG AGCAAATCCA AGAGCTCGAG AAGGATCGAG AGACCCAGAG GAACATCTTG 
GAGCATCAGC TTCTCGAACT TGAGAAGAAG GCCCAAATGA TAGAGTCCCA GAAAGGAGAG 
ATTCAGGACC TGAAGAAGCA GCTGGTTACT CTGGAATGCC TGGCTCTGGA ACGAGAGGAA 
AACCATCACA AGATGGAGTG CCAACAGAAG GCAATCGAGG AGCTGGAGGG CCAGAGGGAA 
ATGCAGAGAG TAGCTCTGAC CCACCTCACA CTGGACCTGG AAGAAAAGAG CCAGGAGCTG 
CAGGCCCAGA GCAGTCAGAT CGACAAGCTG GAGAGCCATA GCACCCTTCT GGCGAAAGAG 
CTCCAGGACA AGGACCAGGA GCTGAAGTCC CAGCAAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAGGAGC GTCTGGCTCA GGACCTAGAG AGGAGGGACC AGGACGTGGT GCTCCAGAGG 
GAAAGGATTC GGGTCCTAGA AGACCAGAGG ACACTGCAGA CCAAGATCCT GCAGGAGGAC 
CTGGGACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGGCTTC CCAGAGGCCA 
CCGATGCCGG AGTGGGCAGA GGAAGGGAAG GGCCAGAGTA AAGCCCAGCG CGGGAGCCTG 
GAGCACCTGA AGCTGATCCT GCGTGACAAG GAGAGGGAGG TAGAATGCCA GCAGGAACGC 
ATCCAGGAAC TGAAGGAGTA TAAGGATCAG CTGGAGCAGC AGCTCCAGGG CCTACACAGG 
AAGGCAGGGG AGACTGGCCT CCTCCTGACT CAGCGAGAGC AGGAGATAGT GGTCCTGCAG 
CGGCATCTGC AGGAAGCCGC AGAACAGGGG GAGCTGAAAG AGCGGTCACT TCAGGGTCAC 
CTGGAAGAGG CCCAGAGAGC CCTGGCCCAG AGGGACCAGG AGCTCGAGGC CCTGCAGCAC 
CAACAGCAGC AGGCCCTGGG GCAGGAGGAG ACTAGGAAGG AAGAGGCAAG CACCCTGCAG 
AGGGCTCTGG AGCAGGCCCA CACGGCGCTG AAAGAGCGCC AGGGAGAGCT TGAGGACCAC 
AAGGAGCATG TGCGAAGGCT CCAGGAGGAG CTGGCCGTGG AGGGACGGCG TGTGCAGGCC 
CTGGAGGAGG TGGTGGGTGA CCTAAGGGCT GAGTCTCGGG AGCAGGAGGA GGCTTTGCTG 
GCCCTCCAGC AACAGGGTGC CGAGCGGGCA CAGGAGCATG AGGCGGAGGT CAGGGGCCTG 
CGGGCCAGCC TCCTGCAGGC AGAGACTGCA CTCAAGGAAC GGGACCTGGC GCTGGAGGCC 
CTGCGAGCCG ATGGCCGGGC CTCCCAGCTT CGGGAGGAGA CAGCCCGGGA CTGGGCCCAA 
GCTCTGCAGG AGGCCCTGAG CAAGGCCCAG GCTGCCGTGC AGGAGAAAGA GCAGCGGCTG 
CTGGGTCAAG CAGAGTTGAG CCGCAGCCTG GAGACCAGCA CCGCCACTTT GCAGGCTGCC 
CTGGACTCTT GCCAGGCACA AGCCCGGCAG CTGGAGGAGG CTCTGAGGAG GCGAGAGGAC 
GAGATCCAGG ACCGGGACCT CCGGCACCAG GAGGCCGTGC AGCAGCTCCA GCGGGCACTT 
GCCCAGAGAG ATGAAGAATT GAGCCATCAG AAGAGACAGG GGCAGCTGCT AGAGCAGTCT 
CTGGCCCGGA GGGCCCGAAA AGATGCCATC CAAGGGAAGC CAGGTCCGGA GCAGGAGAAA 
GAGGAAGAGA TGAGAGGCCT TCGAGAAAGC CTAAGGGAGT TGCAGCTGAC TCTAGCCCAA 
AAGGAAGAGG AGATCCTGGG GCTGAGGGAG GCCCAGCAAA GGAAGAATCT GGAGGACTCA 
CTGCACAGCC ACACAGCGCC CCCAGAGCCC TCTACAGACT TTGCCACCTT AGGGCCCAGG 
CTGCAGCAGG AGCTGGAGCG ACTGCAGACA GCACTGAGGC TAACTGAGGC CAGGGAGATT 
GAGTGGAGGG AGAAGGCCCA GGACTTGGCA CTGTCCCTGG CCCAGAGCAA GGCTAGCGTC 
AGCAGTTTGC AGGAGGCAGC CATGTTCCTA CAGGCCTCTG TTCTGGAGCG GGACTTGGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAGACAGG CTCTGGAGAA GGAGCAGCTC 
CTGAGCTCTA GTTCAACCAG CAGAGCAGAA CAGAGGCCCA GAGAAGAGGT GTCAGAAGTC 
GAGGCTGAGC CTAGTCTTGG GCTGGAGGAG AGGCAGCTCT GGGGACAAAG GCTGGAGTAC 
CTCCAGCAAG CAGTGGCACA GCTGGAGATT GACCGGAGCA GGCTGCAGCA CCACAATGTC 
CAGCTGCGGG CCACCTTGGA GCAGGTGGAG CGAGAGCGCA GGAAGCTGAA GAGGGAGTCC 
ATGCGCGTGT CACGGACAGG CGGCCTGGAG GTTAAGGAAG CTGCGGCTTC GTCCCCCACA 
CAGCAGGATG GAAGGGGAGG ACAGAAGGGC TCCTCAGATG ACAAGCAGAT GGCTGAACTG 
CAGAAAGAGG TGGCCATGCT GCGGGGCCAG CTGGCCTTGG AGCGGAAGCA GAGGCAGGAC 
TACATTGCTC GCTCGGTGCA GACTAGCCGT GAGCTAGCGG GCCTGCACCA CAGCCTCTCC 
CACTCACTGC TGGCCGTGGC CCAGGCCCCT GAAGCCACTG TCCTGGAGGC CGAGACCCGC 
AAGCTGGACG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGCTTT GCTGTGCCCC 
AGCCCCAGCA CTATCCAGGC CATCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635033.1
CDS	212..7024
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_005634976.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634976.3

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGAGAAGG AGCGCTCTTG GCACCAGCAG GAACTGGATA AGGCCCTGGA GAGCCTAGAG 
AAGGAGAAAA TGGAGCTGGA AACGAGGCTG AGGGAACAGC AGGCAGAAGC CGAGGCCATC 
CGGACACAGA GGGAGGAAGA ACGGGCGGAG GCAGAGAGTG CCCTCTGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAGT GTCCCTCCTG GAGACCCTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT AGAGCGGCTG AGGCAGGACA TGAAGGTCCA GAAGTTAAAG 
GAGCAGGAGA CCACTCAGAT CCTGCAGGCC CAGCTCCGGG AGGCTCGGGG GGAGCTGGAG 
CAGGCAGCCC AGCAGAACAG AGATGACCTT GTTGCTGTCC AAGAAGAGTG CGGGGCCCTG 
CTGCAGGCGA AGATGGACCT GCAGAAGCGG GTAGAAGAGT TGAAGTCTCA GCTGGTTTCC 
AGAGATGACT CCCGGAGGCT GGTGGAGCAG GAGGTTCAGG AGAAGCTGAG GGAGGCCCAG 
GAGTATAGCC GAATTCAGAA GGAGCTGGAG AGAGAGAAAG CCAGCCTGAT TCAGTCGCTG 
ATGGAAAAGG AGCAGAGACT CCTTGTTTTA CAAGAAGCTG ACTCTGTTCG ACAACAGGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG TCCCAGGAAG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTTCT GGCTCAGGAA 
GCACAACTGC TGGAGGAGCT GGAGACATCT CAAGTAACAG AGCAGCGGCT GCGAGCTTCT 
TTGCGGGCCC TGGAAGCCAA GGCAGCCCAA GTCCAGCTGC GACTGCGCAG CACAGAGAAC 
CAGTTGGCAG CTCTGGTGGC AGAGCAGCAG CCGGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTGTT CTGTCCTGCA GCAGGCCTTG GGGTTTGCTT GTGAGAGCAG GCCTGAGCTG 
CGTGGCGGGG GAGACTCTGC TTCCCTCTGG GGCCCCGAGC CAGACCAGAA TGGAACTGGG 
ATCCTCCTTA AGAGAGGGCC CCTCCTGACA GCTCTGTCAG CTGAGGCAGT GGCATCTGCC 
CTCCAGAAAC TTCACCAAGA CCTATGGAAG ACTCAGCAGG CCCGGGATGA TCTGCGGGAA 
CAGGCCCTGA AGCTGGAACA GCGTCTCACT GATACAGAGG CCGAGAAGAG TCAGGTCTGC 
ACAGAATTGC AGGATTTGCA GAGACAACTC TCCCAGAACC AGGAAGAGAA ATCCAAGTGG 
GAAGGAAAAC AGATCTCCCT GGAATCTGAG CTGACTGAAC TGCATGGAAC TGTGGCATCA 
TTACAGAGTC GTCTACAGCA AGCAGAGCTG CAGGGACTAG AGGCCCAGAA TGAGCGAAAG 
CTACTGCAGG CAGCCAAGGA GAGCCTGACA GCCCAGGTGG AACGTTTGCA GGCATCTGTG 
GCAGAAGCCA GGGCTCAGGC TGGTGCCACC AGGGCTCTGG AGGAGGACTT GAGAACTGCT 
CGCTCAGCCC TGAAACTCAA GAGTGAGGAA GCAGAGACCG AGCGTGAGCG GGCCCAGGCT 
CTGCAGGAGC AGGGCGAGCT GAAGGTGGCC CAAGGGAAGG CTCTGCAGGA GAATTTAGCT 
ATCCTGGTTC AGACTCTATC CGAAAGAGAA GGGGAGGTGG AGGCTTTGCG GGGAAGTATT 
CAGGAACTGG AGAAACAACG GGAGATGCAA AAGGCTACTT TGGAAGCTCT GTCTCTGGAC 
CTGAAGAAGA AGAGCGAAGA GGTAGATGTG CAACAAGAAC AGATCCAGGA GCTGGAGAAG 
TGCAGGTCCC TTTTAGAAGA TCTGCCTCTG GCCATGCAGG AACAAGAGCA GAGGCTGGTT 
GCACAGAGGG AGCAAATCCA AGAGCTCGAG AAGGATCGAG AGACCCAGAG GAACATCTTG 
GAGCATCAGC TTCTCGAACT TGAGAAGAAG GCCCAAATGA TAGAGTCCCA GAAAGGAGAG 
ATTCAGGACC TGAAGAAGCA GCTGGTTACT CTGGAATGCC TGGCTCTGGA ACGAGAGGAA 
AACCATCACA AGATGGAGTG CCAACAGAAG GCAATCGAGG AGCTGGAGGG CCAGAGGGAA 
ATGCAGAGAG TAGCTCTGAC CCACCTCACA CTGGACCTGG AAGAAAAGAG CCAGGAGCTG 
CAGGCCCAGA GCAGTCAGAT CGACAAGCTG GAGAGCCATA GCACCCTTCT GGCGAAAGAG 
CTCCAGGACA AGGACCAGGA GCTGAAGTCC CAGCAAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAGGAGC GTCTGGCTCA GGACCTAGAG AGGAGGGACC AGGACGTGGT GCTCCAGAGG 
GAAAGGATTC GGGTCCTAGA AGACCAGAGG ACACTGCAGA CCAAGATCCT GCAGGAGGAC 
CTGGGACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGGCTTC CCAGAGGCCA 
CCGATGCCGG AGTGGGCAGA GGAAGGGAAG GGCCAGAGTA AAGCCCAGCG CGGGAGCCTG 
GAGCACCTGA AGCTGATCCT GCGTGACAAG GAGAGGGAGG TAGAATGCCA GCAGGAACGC 
ATCCAGGAAC TGAAGGAGTA TAAGGATCAG CTGGAGCAGC AGCTCCAGGG CCTACACAGG 
AAGGCAGGGG AGACTGGCCT CCTCCTGACT CAGCGAGAGC AGGAGATAGT GGTCCTGCAG 
CGGCATCTGC AGGAAGCCGC AGAACAGGGG GAGCTGAAAG AGCGGTCACT TCAGGGTCAC 
CTGGAAGAGG CCCAGAGAGC CCTGGCCCAG AGGGACCAGG AGCTCGAGGC CCTGCAGCAC 
CAACAGCAGC AGGCCCTGGG GCAGGAGGAG ACTAGGAAGG AAGAGGCAAG CACCCTGCAG 
AGGGCTCTGG AGCAGGCCCA CACGGCGCTG AAAGAGCGCC AGGGAGAGCT TGAGGACCAC 
AAGGAGCATG TGCGAAGGCT CCAGGAGGAG CTGGCCGTGG AGGGACGGCG TGTGCAGGCC 
CTGGAGGAGG TGGTGGGTGA CCTAAGGGCT GAGTCTCGGG AGCAGGAGGA GGCTTTGCTG 
GCCCTCCAGC AACAGGGTGC CGAGCGGGCA CAGGAGCATG AGGCGGAGGT CAGGGGCCTG 
CGGGCCAGCC TCCTGCAGGC AGAGACTGCA CTCAAGGAAC GGGACCTGGC GCTGGAGGCC 
CTGCGAGCCG ATGGCCGGGC CTCCCAGCTT CGGGAGGAGA CAGCCCGGGA CTGGGCCCAA 
GCTCTGCAGG AGGCCCTGAG CAAGGCCCAG GCTGCCGTGC AGGAGAAAGA GCAGCGGCTG 
CTGGGTCAAG CAGAGTTGAG CCGCAGCCTG GAGACCAGCA CCGCCACTTT GCAGGCTGCC 
CTGGACTCTT GCCAGGCACA AGCCCGGCAG CTGGAGGAGG CTCTGAGGAG GCGAGAGGAC 
GAGATCCAGG ACCGGGACCT CCGGCACCAG GAGGCCGTGC AGCAGCTCCA GCGGGCACTT 
GCCCAGAGAG ATGAAGAATT GAGCCATCAG AAGAGACAGG GGCAGCTGCT AGAGCAGTCT 
CTGGCCCGGA GGGCCCGAAA AGATGCCATC CAAGGGAAGC CAGGTCCGGA GCAGGAGAAA 
GAGGAAGAGA TGAGAGGCCT TCGAGAAAGC CTAAGGGAGT TGCAGCTGAC TCTAGCCCAA 
AAGGAAGAGG AGATCCTGGG GCTGAGGGAG GCCCAGCAAA GGAAGAATCT GGAGGACTCA 
CTGCACAGCC ACACAGCGCC CCCAGAGCCC TCTACAGACT TTGCCACCTT AGGGCCCAGG 
CTGCAGCAGG AGCTGGAGCG ACTGCAGACA GCACTGAGGC TAACTGAGGC CAGGGAGATT 
GAGTGGAGGG AGAAGGCCCA GGACTTGGCA CTGTCCCTGG CCCAGAGCAA GGCTAGCGTC 
AGCAGTTTGC AGGAGGCAGC CATGTTCCTA CAGGCCTCTG TTCTGGAGCG GGACTTGGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAGACAGG CTCTGGAGAA GGAGCAGCTC 
CTGAGCTCTA GTTCAACCAG CAGAGCAGAA CAGAGGCCCA GAGAAGAGGT GTCAGAAGTC 
GAGGCTGAGC CTAGTCTTGG GCTGGAGGAG AGGCAGCTCT GGGGACAAAG GCTGGAGTAC 
CTCCAGCAAG CAGTGGCACA GCTGGAGATT GACCGGAGCA GGCTGCAGCA CCACAATGTC 
CAGCTGCGGG CCACCTTGGA GCAGGTGGAG CGAGAGCGCA GGAAGCTGAA GAGGGAGTCC 
ATGCGCGTGT CACGGACAGG CGGCCTGGAG GTTAAGGAAG CTGCGGCTTC GTCCCCCACA 
CAGCAGGATG GAAGGGGAGG ACAGAAGGGC TCCTCAGATG ACAAGCAGAT GGCTGAACTG 
CAGAAAGAGG TGGCCATGCT GCGGGGCCAG CTGGCCTTGG AGCGGAAGCA GAGGCAGGAC 
TACATTGCTC GCTCGGTGCA GACTAGCCGT GAGCTAGCGG GCCTGCACCA CAGCCTCTCC 
CACTCACTGC TGGCCGTGGC CCAGGCCCCT GAAGCCACTG TCCTGGAGGC CGAGACCCGC 
AAGCTGGACG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGCTTT GCTGTGCCCC 
AGCCCCAGCA CTATCCAGGC CATCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005634976.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635033.1 Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

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CloneID	OCe24793
Clone ID Related Accession (Same CDS sequence)	XM_005634976.3 , XM_005634976.2
Accession Version	XM_005634976.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	centrosome-associated protein CEP250 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005634976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGAGAAGG AGCGCTCTTG GCACCAGCAG GAACTGGATA AGGCCCTGGA GAGCCTAGAG 
AAGGAGAAAA TGGAGCTGGA AACGAGGCTG AGGGAACAGC AGGCAGAAGC CGAGGCCATC 
CGGACACAGA GGGAGGAAGA ACGGGCGGAG GCAGAGAGTG CCCTCTGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAGT GTCCCTCCTG GAGACCCTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT AGAGCGGCTG AGGCAGGACA TGAAGGTCCA GAAGTTAAAG 
GAGCAGGAGA CCACTCAGAT CCTGCAGGCC CAGCTCCGGG AGGCTCGGGG GGAGCTGGAG 
CAGGCAGCCC AGCAGAACAG AGATGACCTT GTTGCTGTCC AAGAAGAGTG CGGGGCCCTG 
CTGCAGGCGA AGATGGACCT GCAGAAGCGG GTAGAAGAGT TGAAGTCTCA GCTGGTTTCC 
AGAGATGACT CCCGGAGGCT GGTGGAGCAG GAGGTTCAGG AGAAGCTGAG GGAGGCCCAG 
GAGTATAGCC GAATTCAGAA GGAGCTGGAG AGAGAGAAAG CCAGCCTGAT TCAGTCGCTG 
ATGGAAAAGG AGCAGAGACT CCTTGTTTTA CAAGAAGCTG ACTCTGTTCG ACAACAGGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG TCCCAGGAAG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTTCT GGCTCAGGAA 
GCACAACTGC TGGAGGAGCT GGAGACATCT CAAGTAACAG AGCAGCGGCT GCGAGCTTCT 
TTGCGGGCCC TGGAAGCCAA GGCAGCCCAA GTCCAGCTGC GACTGCGCAG CACAGAGAAC 
CAGTTGGCAG CTCTGGTGGC AGAGCAGCAG CCGGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTGTT CTGTCCTGCA GCAGGCCTTG GGGTTTGCTT GTGAGAGCAG GCCTGAGCTG 
CGTGGCGGGG GAGACTCTGC TTCCCTCTGG GGCCCCGAGC CAGACCAGAA TGGAACTGGG 
ATCCTCCTTA AGAGAGGGCC CCTCCTGACA GCTCTGTCAG CTGAGGCAGT GGCATCTGCC 
CTCCAGAAAC TTCACCAAGA CCTATGGAAG ACTCAGCAGG CCCGGGATGA TCTGCGGGAA 
CAGGCCCTGA AGCTGGAACA GCGTCTCACT GATACAGAGG CCGAGAAGAG TCAGGTCTGC 
ACAGAATTGC AGGATTTGCA GAGACAACTC TCCCAGAACC AGGAAGAGAA ATCCAAGTGG 
GAAGGAAAAC AGATCTCCCT GGAATCTGAG CTGACTGAAC TGCATGGAAC TGTGGCATCA 
TTACAGAGTC GTCTACAGCA AGCAGAGCTG CAGGGACTAG AGGCCCAGAA TGAGCGAAAG 
CTACTGCAGG CAGCCAAGGA GAGCCTGACA GCCCAGGTGG AACGTTTGCA GGCATCTGTG 
GCAGAAGCCA GGGCTCAGGC TGGTGCCACC AGGGCTCTGG AGGAGGACTT GAGAACTGCT 
CGCTCAGCCC TGAAACTCAA GAGTGAGGAA GCAGAGACCG AGCGTGAGCG GGCCCAGGCT 
CTGCAGGAGC AGGGCGAGCT GAAGGTGGCC CAAGGGAAGG CTCTGCAGGA GAATTTAGCT 
ATCCTGGTTC AGACTCTATC CGAAAGAGAA GGGGAGGTGG AGGCTTTGCG GGGAAGTATT 
CAGGAACTGG AGAAACAACG GGAGATGCAA AAGGCTACTT TGGAAGCTCT GTCTCTGGAC 
CTGAAGAAGA AGAGCGAAGA GGTAGATGTG CAACAAGAAC AGATCCAGGA GCTGGAGAAG 
TGCAGGTCCC TTTTAGAAGA TCTGCCTCTG GCCATGCAGG AACAAGAGCA GAGGCTGGTT 
GCACAGAGGG AGCAAATCCA AGAGCTCGAG AAGGATCGAG AGACCCAGAG GAACATCTTG 
GAGCATCAGC TTCTCGAACT TGAGAAGAAG GCCCAAATGA TAGAGTCCCA GAAAGGAGAG 
ATTCAGGACC TGAAGAAGCA GCTGGTTACT CTGGAATGCC TGGCTCTGGA ACGAGAGGAA 
AACCATCACA AGATGGAGTG CCAACAGAAG GCAATCGAGG AGCTGGAGGG CCAGAGGGAA 
ATGCAGAGAG TAGCTCTGAC CCACCTCACA CTGGACCTGG AAGAAAAGAG CCAGGAGCTG 
CAGGCCCAGA GCAGTCAGAT CGACAAGCTG GAGAGCCATA GCACCCTTCT GGCGAAAGAG 
CTCCAGGACA AGGACCAGGA GCTGAAGTCC CAGCAAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAGGAGC GTCTGGCTCA GGACCTAGAG AGGAGGGACC AGGACGTGGT GCTCCAGAGG 
GAAAGGATTC GGGTCCTAGA AGACCAGAGG ACACTGCAGA CCAAGATCCT GCAGGAGGAC 
CTGGGACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGGCTTC CCAGAGGCCA 
CCGATGCCGG AGTGGGCAGA GGAAGGGAAG GGCCAGAGTA AAGCCCAGCG CGGGAGCCTG 
GAGCACCTGA AGCTGATCCT GCGTGACAAG GAGAGGGAGG TAGAATGCCA GCAGGAACGC 
ATCCAGGAAC TGAAGGAGTA TAAGGATCAG CTGGAGCAGC AGCTCCAGGG CCTACACAGG 
AAGGCAGGGG AGACTGGCCT CCTCCTGACT CAGCGAGAGC AGGAGATAGT GGTCCTGCAG 
CGGCATCTGC AGGAAGCCGC AGAACAGGGG GAGCTGAAAG AGCGGTCACT TCAGGGTCAC 
CTGGAAGAGG CCCAGAGAGC CCTGGCCCAG AGGGACCAGG AGCTCGAGGC CCTGCAGCAC 
CAACAGCAGC AGGCCCTGGG GCAGGAGGAG ACTAGGAAGG AAGAGGCAAG CACCCTGCAG 
AGGGCTCTGG AGCAGGCCCA CACGGCGCTG AAAGAGCGCC AGGGAGAGCT TGAGGACCAC 
AAGGAGCATG TGCGAAGGCT CCAGGAGGAG CTGGCCGTGG AGGGACGGCG TGTGCAGGCC 
CTGGAGGAGG TGGTGGGTGA CCTAAGGGCT GAGTCTCGGG AGCAGGAGGA GGCTTTGCTG 
GCCCTCCAGC AACAGGGTGC CGAGCGGGCA CAGGAGCATG AGGCGGAGGT CAGGGGCCTG 
CGGGCCAGCC TCCTGCAGGC AGAGACTGCA CTCAAGGAAC GGGACCTGGC GCTGGAGGCC 
CTGCGAGCCG ATGGCCGGGC CTCCCAGCTT CGGGAGGAGA CAGCCCGGGA CTGGGCCCAA 
GCTCTGCAGG AGGCCCTGAG CAAGGCCCAG GCTGCCGTGC AGGAGAAAGA GCAGCGGCTG 
CTGGGTCAAG CAGAGTTGAG CCGCAGCCTG GAGACCAGCA CCGCCACTTT GCAGGCTGCC 
CTGGACTCTT GCCAGGCACA AGCCCGGCAG CTGGAGGAGG CTCTGAGGAG GCGAGAGGAC 
GAGATCCAGG ACCGGGACCT CCGGCACCAG GAGGCCGTGC AGCAGCTCCA GCGGGCACTT 
GCCCAGAGAG ATGAAGAATT GAGCCATCAG AAGAGACAGG GGCAGCTGCT AGAGCAGTCT 
CTGGCCCGGA GGGCCCGAAA AGATGCCATC CAAGGGAAGC CAGGTCCGGA GCAGGAGAAA 
GAGGAAGAGA TGAGAGGCCT TCGAGAAAGC CTAAGGGAGT TGCAGCTGAC TCTAGCCCAA 
AAGGAAGAGG AGATCCTGGG GCTGAGGGAG GCCCAGCAAA GGAAGAATCT GGAGGACTCA 
CTGCACAGCC ACACAGCGCC CCCAGAGCCC TCTACAGACT TTGCCACCTT AGGGCCCAGG 
CTGCAGCAGG AGCTGGAGCG ACTGCAGACA GCACTGAGGC TAACTGAGGC CAGGGAGATT 
GAGTGGAGGG AGAAGGCCCA GGACTTGGCA CTGTCCCTGG CCCAGAGCAA GGCTAGCGTC 
AGCAGTTTGC AGGAGGCAGC CATGTTCCTA CAGGCCTCTG TTCTGGAGCG GGACTTGGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAGACAGG CTCTGGAGAA GGAGCAGCTC 
CTGAGCTCTA GTTCAACCAG CAGAGCAGAA CAGAGGCCCA GAGAAGAGGT GTCAGAAGTC 
GAGGCTGAGC CTAGTCTTGG GCTGGAGGAG AGGCAGCTCT GGGGACAAAG GCTGGAGTAC 
CTCCAGCAAG CAGTGGCACA GCTGGAGATT GACCGGAGCA GGCTGCAGCA CCACAATGTC 
CAGCTGCGGG CCACCTTGGA GCAGGTGGAG CGAGAGCGCA GGAAGCTGAA GAGGGAGTCC 
ATGCGCGTGT CACGGACAGG CGGCCTGGAG GTTAAGGAAG CTGCGGCTTC GTCCCCCACA 
CAGCAGGATG GAAGGGGAGG ACAGAAGGGC TCCTCAGATG ACAAGCAGAT GGCTGAACTG 
CAGAAAGAGG TGGCCATGCT GCGGGGCCAG CTGGCCTTGG AGCGGAAGCA GAGGCAGGAC 
TACATTGCTC GCTCGGTGCA GACTAGCCGT GAGCTAGCGG GCCTGCACCA CAGCCTCTCC 
CACTCACTGC TGGCCGTGGC CCAGGCCCCT GAAGCCACTG TCCTGGAGGC CGAGACCCGC 
AAGCTGGACG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGCTTT GCTGTGCCCC 
AGCCCCAGCA CTATCCAGGC CATCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635033.1
CDS	198..7010
Translation	METRGPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRTWCQELEKQLEATGGPTPQRWESMEEPDLEQLLVRLEEEQQRCESLAEVNTQLRLHMEKADMVNKALREDVENLTVDWSRARDELRRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHVKLSGSLLTCCLRLTVGTQSRESDGSGRRDGSEPTQLLLLLTKTQELEKEAHERSQELIQLKSRGDLEKAELQDQVTELSALLIQARKQNEEYEKMLGALRETVEILETNHAELMEHEVSLSKNAQEEKLSLQQVIRDITQVLVMVEEGDSMTQGCGRDSSLELDPSGLSSQFDSQDPDKALTLVRSVVTQRRQAVQDLRQQLSACQEAMSSLRQQHNQWEEEGEALRQRLQKLTGERDTLAGQTSDLQGEVESLSKERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSVQGEKEEQQEELHLAVRERERLQETLASLEAKQSESLSELIILREALESCHLEGELLRQEQTEVTAALARAEQSVAELSSSENSLKAEVADLRAATIKLSALNEALALDKVGLNQQLLQLEQENQSVCHRMEATEQARNALQLGLAEAERSREALQEKNTHLEAQLQKAEERGAELQADLRAIQDEKEEIQEKLSEEKERSWHQQELDKALESLEKEKMELETRLREQQAEAEAIRTQREEERAEAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTQILQAQLREARGELEQAAQQNRDDLVAVQEECGALLQAKMDLQKRVEELKSQLVSRDDSRRLVEQEVQEKLREAQEYSRIQKELEREKASLIQSLMEKEQRLLVLQEADSVRQQELSSLRQDMQESQEGQKELSTQVELLKQEVKEKEADFLAQEAQLLEELETSQVTEQRLRASLRALEAKAAQVQLRLRSTENQLAALVAEQQPGHQAQAQLASLCSVLQQALGFACESRPELRGGGDSASLWGPEPDQNGTGILLKRGPLLTALSAEAVASALQKLHQDLWKTQQARDDLREQALKLEQRLTDTEAEKSQVCTELQDLQRQLSQNQEEKSKWEGKQISLESELTELHGTVASLQSRLQQAELQGLEAQNERKLLQAAKESLTAQVERLQASVAEARAQAGATRALEEDLRTARSALKLKSEEAETERERAQALQEQGELKVAQGKALQENLAILVQTLSEREGEVEALRGSIQELEKQREMQKATLEALSLDLKKKSEEVDVQQEQIQELEKCRSLLEDLPLAMQEQEQRLVAQREQIQELEKDRETQRNILEHQLLELEKKAQMIESQKGEIQDLKKQLVTLECLALEREENHHKMECQQKAIEELEGQREMQRVALTHLTLDLEEKSQELQAQSSQIDKLESHSTLLAKELQDKDQELKSQQEQIEELQRQKERLAQDLERRDQDVVLQRERIRVLEDQRTLQTKILQEDLGQIKLSLRERGRELASQRPPMPEWAEEGKGQSKAQRGSLEHLKLILRDKEREVECQQERIQELKEYKDQLEQQLQGLHRKAGETGLLLTQREQEIVVLQRHLQEAAEQGELKERSLQGHLEEAQRALAQRDQELEALQHQQQQALGQEETRKEEASTLQRALEQAHTALKERQGELEDHKEHVRRLQEELAVEGRRVQALEEVVGDLRAESREQEEALLALQQQGAERAQEHEAEVRGLRASLLQAETALKERDLALEALRADGRASQLREETARDWAQALQEALSKAQAAVQEKEQRLLGQAELSRSLETSTATLQAALDSCQAQARQLEEALRRREDEIQDRDLRHQEAVQQLQRALAQRDEELSHQKRQGQLLEQSLARRARKDAIQGKPGPEQEKEEEMRGLRESLRELQLTLAQKEEEILGLREAQQRKNLEDSLHSHTAPPEPSTDFATLGPRLQQELERLQTALRLTEAREIEWREKAQDLALSLAQSKASVSSLQEAAMFLQASVLERDLEQQRLQDELELTRQALEKEQLLSSSSTSRAEQRPREEVSEVEAEPSLGLEERQLWGQRLEYLQQAVAQLEIDRSRLQHHNVQLRATLEQVERERRKLKRESMRVSRTGGLEVKEAAASSPTQQDGRGGQKGSSDDKQMAELQKEVAMLRGQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSPSTIQAISR

Target ORF information:

RefSeq Version	XM_005634976.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005634976.2

ORF Insert Sequence:

ATGGAGACGC GAGGCCCTGG GTTGAACAAT ATGAAGCCCC AGTCACTGCA GTTGGTGCTG 
GAGGAGCAAG TACTAGCACT ACAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGGCAAGCAA CCCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GACCTGGTGC CAAGAGCTGG AGAAGCAGCT GGAAGCCACT 
GGGGGACCGA CTCCTCAGAG ATGGGAAAGC ATGGAGGAGC CAGACCTGGA ACAGTTGCTG 
GTCCGTTTGG AGGAGGAACA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACATGGTG AATAAAGCAC TTCGGGAAGA TGTGGAAAAC 
CTGACAGTGG ACTGGAGCCG AGCCCGGGAT GAGCTCAGGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTTGGTCTG 
TGGCGGGAGG TGGTGACCTT CCGACGCCAC TTCCTGGAGA TGAAGTCAGC TACTGACAGA 
GATCTGACAG AGCTCAAGGC TGAACATGTG AAGCTTTCAG GGTCCCTGTT GACATGTTGT 
CTGCGCTTGA CTGTGGGCAC CCAGTCTCGA GAGTCGGATG GATCTGGGAG ACGGGATGGG 
AGTGAGCCAA CCCAGCTGCT GCTACTACTG ACCAAGACCC AGGAGCTGGA GAAGGAAGCG 
CATGAAAGGA GCCAGGAGCT AATACAGCTG AAGAGTCGGG GCGATCTGGA GAAGGCTGAG 
CTGCAGGATC AGGTGACGGA GCTCTCTGCT CTGCTGATCC AGGCTCGGAA GCAAAATGAA 
GAGTATGAGA AGATGTTAGG GGCCCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAT 
GCAGAATTAA TGGAACATGA GGTATCTCTC AGTAAGAATG CCCAAGAGGA GAAGCTGTCT 
TTACAGCAGG TGATCAGGGA TATAACCCAG GTACTGGTCA TGGTGGAAGA AGGGGACAGT 
ATGACCCAAG GCTGTGGTCG AGACAGCTCC TTAGAATTGG ACCCTAGTGG CCTCTCATCC 
CAGTTTGATT CCCAGGACCC AGACAAGGCC CTTACTCTGG TGCGTTCAGT GGTGACTCAA 
AGACGCCAGG CTGTGCAGGA CCTAAGGCAG CAGCTTTCGG CCTGTCAGGA AGCTATGAGC 
TCTTTGCGGC AGCAGCATAA TCAGTGGGAG GAGGAGGGTG AGGCCTTAAG ACAGCGTCTG 
CAGAAGCTCA CCGGGGAACG CGACACTCTG GCAGGGCAGA CCTCGGACCT ACAGGGAGAG 
GTGGAGTCTC TCAGCAAGGA GCGAGAGCTC CTGCAGAAGA CCAGGGAGGA GCTGCAGCAG 
CAGTTGGAGG TGCTGGAGCA GGAGGCATGG CGGCTGCGAA GGACAAACAT GGAGCTTCAG 
TTGCAGGGGG ATTCTGTTCA GGGTGAGAAG GAGGAGCAGC AGGAGGAGCT GCACCTGGCT 
GTCCGTGAAA GGGAGCGCCT TCAGGAGACA CTAGCAAGTC TGGAAGCCAA ACAGTCAGAA 
TCACTCAGTG AATTGATCAT TCTTCGGGAA GCCCTGGAGT CTTGTCACCT GGAAGGGGAG 
CTGCTGAGGC AAGAGCAAAC AGAAGTGACT GCGGCGCTGG CCAGGGCAGA ACAGTCAGTT 
GCAGAGCTGT CAAGTTCTGA AAACAGCCTG AAGGCCGAGG TTGCTGATCT TCGGGCTGCA 
ACCATCAAGC TCAGCGCCTT AAATGAGGCT TTGGCCTTGG ATAAGGTTGG ACTGAACCAG 
CAGCTTCTCC AGTTAGAACA AGAGAACCAA TCTGTGTGCC ACAGAATGGA AGCAACAGAG 
CAGGCAAGAA ACGCTTTGCA GTTGGGCCTG GCAGAGGCCG AGAGGAGCAG GGAAGCCCTA 
CAGGAAAAGA ACACTCACCT GGAGGCACAG CTGCAGAAGG CAGAGGAGAG GGGGGCTGAG 
CTGCAGGCAG ATCTCAGGGC CATCCAAGAT GAGAAGGAAG AAATTCAAGA GAAACTAAGT 
GAGGAGAAGG AGCGCTCTTG GCACCAGCAG GAACTGGATA AGGCCCTGGA GAGCCTAGAG 
AAGGAGAAAA TGGAGCTGGA AACGAGGCTG AGGGAACAGC AGGCAGAAGC CGAGGCCATC 
CGGACACAGA GGGAGGAAGA ACGGGCGGAG GCAGAGAGTG CCCTCTGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAGT GTCCCTCCTG GAGACCCTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT AGAGCGGCTG AGGCAGGACA TGAAGGTCCA GAAGTTAAAG 
GAGCAGGAGA CCACTCAGAT CCTGCAGGCC CAGCTCCGGG AGGCTCGGGG GGAGCTGGAG 
CAGGCAGCCC AGCAGAACAG AGATGACCTT GTTGCTGTCC AAGAAGAGTG CGGGGCCCTG 
CTGCAGGCGA AGATGGACCT GCAGAAGCGG GTAGAAGAGT TGAAGTCTCA GCTGGTTTCC 
AGAGATGACT CCCGGAGGCT GGTGGAGCAG GAGGTTCAGG AGAAGCTGAG GGAGGCCCAG 
GAGTATAGCC GAATTCAGAA GGAGCTGGAG AGAGAGAAAG CCAGCCTGAT TCAGTCGCTG 
ATGGAAAAGG AGCAGAGACT CCTTGTTTTA CAAGAAGCTG ACTCTGTTCG ACAACAGGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG TCCCAGGAAG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTTCT GGCTCAGGAA 
GCACAACTGC TGGAGGAGCT GGAGACATCT CAAGTAACAG AGCAGCGGCT GCGAGCTTCT 
TTGCGGGCCC TGGAAGCCAA GGCAGCCCAA GTCCAGCTGC GACTGCGCAG CACAGAGAAC 
CAGTTGGCAG CTCTGGTGGC AGAGCAGCAG CCGGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTGTT CTGTCCTGCA GCAGGCCTTG GGGTTTGCTT GTGAGAGCAG GCCTGAGCTG 
CGTGGCGGGG GAGACTCTGC TTCCCTCTGG GGCCCCGAGC CAGACCAGAA TGGAACTGGG 
ATCCTCCTTA AGAGAGGGCC CCTCCTGACA GCTCTGTCAG CTGAGGCAGT GGCATCTGCC 
CTCCAGAAAC TTCACCAAGA CCTATGGAAG ACTCAGCAGG CCCGGGATGA TCTGCGGGAA 
CAGGCCCTGA AGCTGGAACA GCGTCTCACT GATACAGAGG CCGAGAAGAG TCAGGTCTGC 
ACAGAATTGC AGGATTTGCA GAGACAACTC TCCCAGAACC AGGAAGAGAA ATCCAAGTGG 
GAAGGAAAAC AGATCTCCCT GGAATCTGAG CTGACTGAAC TGCATGGAAC TGTGGCATCA 
TTACAGAGTC GTCTACAGCA AGCAGAGCTG CAGGGACTAG AGGCCCAGAA TGAGCGAAAG 
CTACTGCAGG CAGCCAAGGA GAGCCTGACA GCCCAGGTGG AACGTTTGCA GGCATCTGTG 
GCAGAAGCCA GGGCTCAGGC TGGTGCCACC AGGGCTCTGG AGGAGGACTT GAGAACTGCT 
CGCTCAGCCC TGAAACTCAA GAGTGAGGAA GCAGAGACCG AGCGTGAGCG GGCCCAGGCT 
CTGCAGGAGC AGGGCGAGCT GAAGGTGGCC CAAGGGAAGG CTCTGCAGGA GAATTTAGCT 
ATCCTGGTTC AGACTCTATC CGAAAGAGAA GGGGAGGTGG AGGCTTTGCG GGGAAGTATT 
CAGGAACTGG AGAAACAACG GGAGATGCAA AAGGCTACTT TGGAAGCTCT GTCTCTGGAC 
CTGAAGAAGA AGAGCGAAGA GGTAGATGTG CAACAAGAAC AGATCCAGGA GCTGGAGAAG 
TGCAGGTCCC TTTTAGAAGA TCTGCCTCTG GCCATGCAGG AACAAGAGCA GAGGCTGGTT 
GCACAGAGGG AGCAAATCCA AGAGCTCGAG AAGGATCGAG AGACCCAGAG GAACATCTTG 
GAGCATCAGC TTCTCGAACT TGAGAAGAAG GCCCAAATGA TAGAGTCCCA GAAAGGAGAG 
ATTCAGGACC TGAAGAAGCA GCTGGTTACT CTGGAATGCC TGGCTCTGGA ACGAGAGGAA 
AACCATCACA AGATGGAGTG CCAACAGAAG GCAATCGAGG AGCTGGAGGG CCAGAGGGAA 
ATGCAGAGAG TAGCTCTGAC CCACCTCACA CTGGACCTGG AAGAAAAGAG CCAGGAGCTG 
CAGGCCCAGA GCAGTCAGAT CGACAAGCTG GAGAGCCATA GCACCCTTCT GGCGAAAGAG 
CTCCAGGACA AGGACCAGGA GCTGAAGTCC CAGCAAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAGGAGC GTCTGGCTCA GGACCTAGAG AGGAGGGACC AGGACGTGGT GCTCCAGAGG 
GAAAGGATTC GGGTCCTAGA AGACCAGAGG ACACTGCAGA CCAAGATCCT GCAGGAGGAC 
CTGGGACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGGCTTC CCAGAGGCCA 
CCGATGCCGG AGTGGGCAGA GGAAGGGAAG GGCCAGAGTA AAGCCCAGCG CGGGAGCCTG 
GAGCACCTGA AGCTGATCCT GCGTGACAAG GAGAGGGAGG TAGAATGCCA GCAGGAACGC 
ATCCAGGAAC TGAAGGAGTA TAAGGATCAG CTGGAGCAGC AGCTCCAGGG CCTACACAGG 
AAGGCAGGGG AGACTGGCCT CCTCCTGACT CAGCGAGAGC AGGAGATAGT GGTCCTGCAG 
CGGCATCTGC AGGAAGCCGC AGAACAGGGG GAGCTGAAAG AGCGGTCACT TCAGGGTCAC 
CTGGAAGAGG CCCAGAGAGC CCTGGCCCAG AGGGACCAGG AGCTCGAGGC CCTGCAGCAC 
CAACAGCAGC AGGCCCTGGG GCAGGAGGAG ACTAGGAAGG AAGAGGCAAG CACCCTGCAG 
AGGGCTCTGG AGCAGGCCCA CACGGCGCTG AAAGAGCGCC AGGGAGAGCT TGAGGACCAC 
AAGGAGCATG TGCGAAGGCT CCAGGAGGAG CTGGCCGTGG AGGGACGGCG TGTGCAGGCC 
CTGGAGGAGG TGGTGGGTGA CCTAAGGGCT GAGTCTCGGG AGCAGGAGGA GGCTTTGCTG 
GCCCTCCAGC AACAGGGTGC CGAGCGGGCA CAGGAGCATG AGGCGGAGGT CAGGGGCCTG 
CGGGCCAGCC TCCTGCAGGC AGAGACTGCA CTCAAGGAAC GGGACCTGGC GCTGGAGGCC 
CTGCGAGCCG ATGGCCGGGC CTCCCAGCTT CGGGAGGAGA CAGCCCGGGA CTGGGCCCAA 
GCTCTGCAGG AGGCCCTGAG CAAGGCCCAG GCTGCCGTGC AGGAGAAAGA GCAGCGGCTG 
CTGGGTCAAG CAGAGTTGAG CCGCAGCCTG GAGACCAGCA CCGCCACTTT GCAGGCTGCC 
CTGGACTCTT GCCAGGCACA AGCCCGGCAG CTGGAGGAGG CTCTGAGGAG GCGAGAGGAC 
GAGATCCAGG ACCGGGACCT CCGGCACCAG GAGGCCGTGC AGCAGCTCCA GCGGGCACTT 
GCCCAGAGAG ATGAAGAATT GAGCCATCAG AAGAGACAGG GGCAGCTGCT AGAGCAGTCT 
CTGGCCCGGA GGGCCCGAAA AGATGCCATC CAAGGGAAGC CAGGTCCGGA GCAGGAGAAA 
GAGGAAGAGA TGAGAGGCCT TCGAGAAAGC CTAAGGGAGT TGCAGCTGAC TCTAGCCCAA 
AAGGAAGAGG AGATCCTGGG GCTGAGGGAG GCCCAGCAAA GGAAGAATCT GGAGGACTCA 
CTGCACAGCC ACACAGCGCC CCCAGAGCCC TCTACAGACT TTGCCACCTT AGGGCCCAGG 
CTGCAGCAGG AGCTGGAGCG ACTGCAGACA GCACTGAGGC TAACTGAGGC CAGGGAGATT 
GAGTGGAGGG AGAAGGCCCA GGACTTGGCA CTGTCCCTGG CCCAGAGCAA GGCTAGCGTC 
AGCAGTTTGC AGGAGGCAGC CATGTTCCTA CAGGCCTCTG TTCTGGAGCG GGACTTGGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAGACAGG CTCTGGAGAA GGAGCAGCTC 
CTGAGCTCTA GTTCAACCAG CAGAGCAGAA CAGAGGCCCA GAGAAGAGGT GTCAGAAGTC 
GAGGCTGAGC CTAGTCTTGG GCTGGAGGAG AGGCAGCTCT GGGGACAAAG GCTGGAGTAC 
CTCCAGCAAG CAGTGGCACA GCTGGAGATT GACCGGAGCA GGCTGCAGCA CCACAATGTC 
CAGCTGCGGG CCACCTTGGA GCAGGTGGAG CGAGAGCGCA GGAAGCTGAA GAGGGAGTCC 
ATGCGCGTGT CACGGACAGG CGGCCTGGAG GTTAAGGAAG CTGCGGCTTC GTCCCCCACA 
CAGCAGGATG GAAGGGGAGG ACAGAAGGGC TCCTCAGATG ACAAGCAGAT GGCTGAACTG 
CAGAAAGAGG TGGCCATGCT GCGGGGCCAG CTGGCCTTGG AGCGGAAGCA GAGGCAGGAC 
TACATTGCTC GCTCGGTGCA GACTAGCCGT GAGCTAGCGG GCCTGCACCA CAGCCTCTCC 
CACTCACTGC TGGCCGTGGC CCAGGCCCCT GAAGCCACTG TCCTGGAGGC CGAGACCCGC 
AAGCTGGACG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGCTTT GCTGTGCCCC 
AGCCCCAGCA CTATCCAGGC CATCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CEP250
Entrez Gene ID	485851
Full Name	centrosomal protein 250
General protein information	Preferred Names centrosomal protein 250 Names centrosome-associated protein CEP250
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Gallus gallus (chicken)	LOC100858952	XP_003642497.2
Homo sapiens (human)	CEP250	NP_009117.2
Pan troglodytes (chimpanzee)	CEP250	XP_003316929.1
Mus musculus (house mouse)	Cep250	NP_001123471.1
Bos taurus (cattle)	CEP250	XP_005215062.1
Gallus gallus (chicken)	CEP250	XP_417323.4
	CEP250	XP_001099508.1
Canis lupus familiaris (dog)	CEP250	XP_542975.3
Rattus norvegicus (Norway rat)	Cep250	XP_006235436.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CEP250 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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