CEP250 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_007186.5	NP_009117.2	centrosome-associated protein CEP250 isoform 1
NM_007186.6	NP_009117.2	centrosome-associated protein CEP250 isoform 1
XM_006723691.1	XP_006723754.1	centrosome-associated protein CEP250 isoform X1
XM_005260263.4	XP_005260320.1	centrosome-associated protein CEP250 isoform X3
XM_017027619.1	XP_016883108.1	centrosome-associated protein CEP250 isoform X10
XM_011528518.2	XP_011526820.1	centrosome-associated protein CEP250 isoform X7
XM_006723690.3	XP_006723753.1	centrosome-associated protein CEP250 isoform X1
NM_001318219.1	NP_001305148.1	centrosome-associated protein CEP250 isoform 2
XM_006723690.4	XP_006723753.1	centrosome-associated protein CEP250 isoform X1
XM_006723692.4	XP_006723755.1	centrosome-associated protein CEP250 isoform X1
XM_017027617.1	XP_016883106.1	centrosome-associated protein CEP250 isoform X6
XM_017027618.1	XP_016883107.1	centrosome-associated protein CEP250 isoform X9
XM_006723692.3	XP_006723755.1	centrosome-associated protein CEP250 isoform X1
XM_005260264.4	XP_005260321.1	centrosome-associated protein CEP250 isoform X4
XM_005260262.4	XP_005260319.1	centrosome-associated protein CEP250 isoform X1
XM_011528517.2	XP_011526819.1	centrosome-associated protein CEP250 isoform X5
XM_006723693.3	XP_006723756.1	centrosome-associated protein CEP250 isoform X1
XM_006723693.4	XP_006723756.1	centrosome-associated protein CEP250 isoform X1
XM_006723694.3	XP_006723757.1	centrosome-associated protein CEP250 isoform X2
XM_011528518.3	XP_011526820.1	centrosome-associated protein CEP250 isoform X7
XM_011528519.2	XP_011526821.1	centrosome-associated protein CEP250 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu64831	XM_011528517.2 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1301.30 $1859.00
OHu92896	XM_017027617.1 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu52877	XM_005260263.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu101849	NM_001318219.1 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu23771	NM_007186.5 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	NM_007186.6 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723692.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723693.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723691.1 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723690.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_005260262.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52875	XM_006723694.3 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52878	XM_005260264.4 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu64832	XM_011528518.3 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu64833	XM_011528519.2 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu92897	XM_017027618.1 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu92898	XM_017027619.1 Latest version!	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu52874	XM_006723690.3	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723692.3	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu52874	XM_006723693.3	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu64832	XM_011528518.2	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

CEP250 ( XM_011528517.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526819.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu64831
Clone ID Related Accession (Same CDS sequence)	XM_011528517.2
Accession Version	XM_011528517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526819.1
CDS	401..7468
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKE

Target ORF information:

RefSeq Version	XM_011528517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528517.2

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_017027617.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883106.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

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CloneID	OHu92896
Clone ID Related Accession (Same CDS sequence)	XM_017027617.1
Accession Version	XM_017027617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGTCT CAGGAGTGGA GGCTGAGCCT 
AGTCCTGATG GAATGGAGAA GCAGTCATGG AGACAAAGGC TTGAACACCT GCAGCAAGCA 
GTGGCCCGGC TGGAGATTGA CAGGAGCAGG CTGCAGCGCC ACAATGTCCA GCTGCGGAGT 
ACCTTGGAGC AGGTGGAGCG AGAACGGAGG AAGCTGAAGA GGGAGGCCAT GCGTGCGGCC 
CAGGCAGGGT CCCTAGAGAT CAGCAAGGCC ACGGCTTCTT CACCCACACA GCAGGATGGG 
AGAGGACAGA AGAACTCAGA TGCCAAGTGT GTGGCTGAAC TGCAGAAAGA GGGCAGAGCA 
CATGGCAAGT GGCATGCTGA CTCAGACACC GGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883106.1
CDS	401..7399
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEGRAHGKWHADSDTGH

Target ORF information:

RefSeq Version	XM_017027617.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027617.1

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGTCT CAGGAGTGGA GGCTGAGCCT 
AGTCCTGATG GAATGGAGAA GCAGTCATGG AGACAAAGGC TTGAACACCT GCAGCAAGCA 
GTGGCCCGGC TGGAGATTGA CAGGAGCAGG CTGCAGCGCC ACAATGTCCA GCTGCGGAGT 
ACCTTGGAGC AGGTGGAGCG AGAACGGAGG AAGCTGAAGA GGGAGGCCAT GCGTGCGGCC 
CAGGCAGGGT CCCTAGAGAT CAGCAAGGCC ACGGCTTCTT CACCCACACA GCAGGATGGG 
AGAGGACAGA AGAACTCAGA TGCCAAGTGT GTGGCTGAAC TGCAGAAAGA GGGCAGAGCA 
CATGGCAAGT GGCATGCTGA CTCAGACACC GGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005260263.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260320.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

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CloneID	OHu52877
Clone ID Related Accession (Same CDS sequence)	XM_005260263.4
Accession Version	XM_005260263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260263.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGGCAG AGCACATGGC 
AAGTGGCATG CTGACTCAGA CACCGGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260320.1
CDS	401..7513
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEGRAHGKWHADSDTGH

Target ORF information:

RefSeq Version	XM_005260263.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260263.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGGCAG AGCACATGGC 
AAGTGGCATG CTGACTCAGA CACCGGGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( NM_001318219.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305148.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant 2, mRNA.

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CloneID	OHu101849
Clone ID Related Accession (Same CDS sequence)	NM_001318219.1
Accession Version	NM_001318219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF049105.1, BC144706.1, FO393401.1 and AW083819.1. On Dec 30, 2015 this sequence version replaced XM_005260265.2. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG CAGAGCAGGC GAGAAATGCT TTGCAGGTCG ACCTGGCGGA GGCAGAGAAG 
AGGAGGGAAG CCCTGTGGGA AAAGAACACT CACCTGGAGG CTCAGCTGCA GAAAGCTGAG 
GAGGCTGGGG CTGAGCTGCA GGCAGATCTC AGGGACATCC AAGAAGAGAA GGAAGAAATT 
CAAAAGAAAC TAAGTGAGTC ACGTCACCAG CAGGAGGCAG CCACGACTCA GCTGGAGCAG 
CTACATCAGG AGGCAAAGCG ACAGGAAGAA GTGCTTGCCA GGGCAGTCCA GGAGAAGGAG 
GCCCTAGTAC GAGAGAAAGC GGCTCTAGAG GTGCGGCTGC AGGCCGTGGA GCGTGACCGG 
CAGGACCTCG CTGAACAACT ACAGGGGCTC AGCTCAGCCA AGGAGCTACT GGAGAGCAGT 
CTGTTTGAAG CCCAACAACA AAATTCTGTG ATAGAGGTCA CCAAGGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACTCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACACTGAACG GAGTCAGGCA GAGCAGGAGC GGGATGCTGC AGCCAGACAG 
CTGGCCCAGG CTGAGCAAGA AGGGAAGACT GCCTTGGAGC AGCAGAAGGC AGCCCATGAG 
AAAGAGGTGA ACCAGCTCCG GGAGAAATGG GAGAAGGAGC GCTCCTGGCA CCAGCAGGAG 
CTGGCAAAGG CTCTGGAGAG CTTAGAAAGG GAAAAAATGG AGCTGGAAAT GAGGCTAAAG 
GAGCAGCAGA CAGAAATGGA GGCCATCCAG GCCCAGAGGG AAGAAGAACG GACCCAGGCA 
GAGAGTGCCC TATGCCAGAT GCAGCTGGAA ACAGAGAAGG AGAGAGTATC CCTCCTGGAG 
ACACTGCTGC AGACGCAGAA GGAGCTAGCA GATGCCAGCC AACAACTGGA ACGACTGAGG 
CAGGACATGA AAGTCCAGAA ATTAAAGGAG CAGGAGACCA CTGGGATACT ACAGACCCAG 
CTCCAGGAGG CTCAACGGGA GCTGAAGGAG GCAGCCCGGC AGCACAGAGA TGACCTTGCT 
GCCCTCCAAG AAGAGAGCAG CTCCCTGCTG CAGGATAAGA TGGACCTGCA GAAGCAGGTG 
GAGGACTTGA AGTCTCAGCT GGTGGCCCAG GATGACTCCC AGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGAGA GACCCAGGAG TATAACCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGACTCT GTCACTGATG GAAAAGGAAC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTATTCGACA ACAAGAGCTG AGTGCCCTGC GCCAGGACAT GCAGGAGGCC 
CAGGGAGAAC AGAAAGAGCT CAGTGCTCAG ATGGAATTAC TAAGGCAAGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC CCAGGAAGCA CAGCTGCTGG AGGAGCTGGA GGCGTCTCAT 
ATCACGGAGC AGCAGCTGCG AGCCTCCTTG TGGGCCCAGG AAGCCAAGGC AGCCCAACTA 
CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG CTAGAAGCGC TGGCCGCAGA GCAGCAGCCC 
GGGAACCAGG CCCAGGCCCA GGCCCAGCTG GCCAGCCTCT ACTCTGCCCT GCAGCAGGCC 
CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG CTGAGTGGTG GGGGAGACTC TGCTCCTTCC 
GTCTGGGGCC TTGAGCCAGA CCAGAATGGA GCTAGGAGCC TCTTTAAGAG AGGGCCCCTG 
CTGACTGCTC TCTCCGCTGA GGCAGTAGCA TCTGCCCTCC ACAAGCTTCA TCAAGACCTG 
TGGAAGACTC AACAGACCCG GGATGTTCTG AGGGATCAGG TCCAGAAACT GGAAGAGCGT 
CTAACTGATA CTGAGGCTGA GAAGAGCCAG GTCCACACAG AGTTGCAGGA TCTGCAGAGA 
CAGCTCTCCC AGAATCAGGA AGAGAAATCC AAGTGGGAAG GAAAGCAGAA CTCCCTAGAA 
TCTGAGCTGA TGGAACTACA TGAAACTATG GCATCCTTAC AGAGTCGCCT GCGGAGAGCA 
GAGCTACAGC GAATGGAAGC CCAGGGTGAG CGAGAGTTAC TTCAGGCAGC CAAGGAGAAC 
CTGACAGCCC AGGTGGAACA CCTGCAAGCA GCTGTCGTAG AAGCCAGGGC TCAGGCAAGT 
GCTGCTGGCA TCCTGGAAGA AGACCTGAGA ACGGCTCGCT CAGCACTGAA GCTGAAAAAT 
GAGGAAGTAG AGAGTGAGCG TGAGAGAGCC CAGGCTCTGC AAGAGCAGGG CGAACTGAAG 
GTGGCCCAAG GGAAGGCTCT GCAAGAGAAT TTGGCCCTCC TGACCCAGAC CCTAGCTGAA 
AGAGAAGAGG AGGTGGAGAC TCTGCGGGGA CAAATCCAGG AACTGGAGAA GCAACGGGAA 
ATGCAGAAGG CTGCTTTGGA ATTGCTGTCT CTGGACCTGA AGAAGAGGAA CCAAGAGGTA 
GATCTGCAGC AAGAACAGAT TCAGGAGCTA GAGAAGTGTA GGTCTGTTTT AGAGCATCTG 
CCCATGGCCG TCCAGGAGCG AGAGCAGAAG CTGACTGTGC AGAGGGAGCA GATCAGAGAG 
CTCGAGAAGG ATCGGGAGAC TCAGAGGAAC GTCTTGGAGC ATCAGCTTCT AGAACTTGAG 
AAGAAAGACC AAATGATTGA GTCCCAGAGA GGACAGGTTC AGGACCTGAA AAAGCAGTTG 
GTTACTCTGG AATGCCTGGC CCTGGAACTG GAGGAAAACC ATCACAAGAT GGAGTGCCAG 
CAAAAACTGA TCAAGGAGCT GGAGGGCCAG AGGGAAACCC AGAGAGTGGC TTTGACCCAC 
CTTACGCTGG ACCTAGAAGA AAGGAGCCAG GAGCTGCAGG CACAAAGCAG CCAGATCCAT 
GACCTGGAGA GCCACAGCAC CGTTCTGGCA AGAGAGCTGC AGGAGAGGGA CCAGGAGGTG 
AAGTCTCAGC GAGAACAGAT CGAGGAGCTG CAGAGGCAGA AAGAGCATCT GACTCAGGAT 
CTCGAGAGGA GAGACCAGGA GCTGATGCTG CAGAAGGAGA GGATTCAGGT TCTCGAGGAT 
CAGAGGACCC GGCAGACCAA GATCCTGGAG GAGGACCTGG AACAGATCAA GCTGTCCTTG 
AGAGAGCGAG GCCGGGAGCT GACCACTCAG AGGCAGCTGA TGCAGGAACG GGCAGAGGAA 
GGGAAGGGCC CAAGTAAAGC ACAGCGCGGG AGCCTAGAGC ACATGAAGCT GATCCTGCGT 
GATAAGGAGA AGGAGGTGGA ATGTCAGCAG GAGCATATCC ATGAACTCCA GGAGCTCAAA 
GACCAGCTGG AGCAGCAGCT CCAGGGCCTG CACAGGAAGG TAGGTGAGAC CAGCCTCCTC 
CTGTCCCAGC GAGAGCAGGA AATAGTGGTC CTGCAGCAGC AACTGCAGGA AGCCAGGGAA 
CAAGGGGAGC TGAAGGAGCA GTCACTTCAG AGTCAACTGG ATGAGGCCCA GAGAGCCCTA 
GCCCAGAGGG ACCAGGAACT GGAGGCTCTG CAGCAAGAAC AGCAGCAGGC CCAGGGACAG 
GAGGAGAGGG TGAAGGAAAA GGCAGACGCC CTCCAGGGAG CTCTGGAGCA AGCCCATATG 
ACACTGAAGG AGCGTCATGG AGAGCTTCAG GACCACAAGG AACAGGCACG AAGGCTGGAG 
GAAGAGCTGG CAGTGGAGGG ACGGCGGGTC CAGGCCCTGG AGGAGGTGCT GGGAGACCTA 
AGGGCTGAGT CTCGGGAACA GGAGAAAGCT CTGTTGGCCC TCCAGCAGCA GTGTGCTGAG 
CAGGCACAGG AGCATGAGGT GGAGACCAGG GCCCTGCAGG ACAGCTGGCT GCAGGCCCAG 
GCAGTGCTCA AGGAACGGGA CCAGGAGCTG GAAGCTCTGC GGGCAGAAAG TCAGTCCTCC 
CGGCATCAGG AGGAGGCTGC CCGGGCCCGG GCTGAGGCTC TGCAGGAGGC CCTTGGCAAG 
GCTCATGCTG CCCTGCAGGG GAAAGAGCAG CATCTCCTCG AGCAGGCAGA ATTGAGCCGC 
AGTCTGGAGG CCAGCACTGC AACCCTGCAA GCCTCCCTGG ATGCCTGCCA GGCACACAGT 
CGGCAGCTGG AGGAGGCTCT GAGGATACAA GAAGGTGAGA TCCAGGACCA GGATCTCCGA 
TACCAGGAGG ATGTGCAGCA GCTGCAGCAG GCACTTGCCC AGAGGGATGA AGAGCTGAGA 
CATCAGCAGG AACGGGAGCA GCTGCTGGAG AAGTCTCTGG CCCAGAGGGT CCAAGAGAAT 
ATGATCCAAG AGAAGCAGAA TCTGGGGCAA GAGAGAGAAG AGGAGGAGAT AAGGGGCCTT 
CATCAGAGTG TAAGGGAGCT ACAGCTGACT CTAGCCCAAA AGGAACAGGA GATTCTGGAG 
CTGAGGGAGA CCCAGCAAAG GAACAACCTG GAAGCCTTAC CCCACAGCCA CAAAACCTCC 
CCAATGGAGG AACAATCTCT AAAACTTGAT TCTTTAGAGC CCAGGCTGCA GCGGGAGCTG 
GAGCGGCTAC AGGCAGCCCT GAGACAGACA GAAGCCAGGG AGATTGAGTG GAGGGAGAAG 
GCCCAGGACT TGGCACTCTC CCTAGCGCAG ACCAAGGCCA GTGTCAGCAG TCTGCAGGAG 
GTAGCCATGT TCCTACAAGC CTCTGTCCTG GAGCGGGACT CAGAACAGCA AAGGCTGCAG 
GATGAACTGG AGCTCACCAG ACGGGCTCTG GAGAAGGAGC GGCTACACAG CCCAGGTGCA 
ACCAGCACAG CAGAACTGGG GTCCAGAGGG GAGCAGGGTG TGCAGCTGGG AGAGGTCTCA 
GGAGTGGAGG CTGAGCCTAG TCCTGATGGA ATGGAGAAGC AGTCATGGAG ACAAAGGCTT 
GAACACCTGC AGCAAGCAGT GGCCCGGCTG GAGATTGACA GGAGCAGGCT GCAGCGCCAC 
AATGTCCAGC TGCGGAGTAC CTTGGAGCAG GTGGAGCGAG AACGGAGGAA GCTGAAGAGG 
GAGGCCATGC GTGCGGCCCA GGCAGGGTCC CTAGAGATCA GCAAGGCCAC GGCTTCTTCA 
CCCACACAGC AGGATGGGAG AGGACAGAAG AACTCAGATG CCAAGTGTGT GGCTGAACTG 
CAGAAAGAGG TGGTCCTGCT GCAAGCTCAG CTGACTTTGG AGCGGAAGCA GAAGCAGGAC 
TACATCACCC GCTCAGCACA GACCAGCCGT GAGCTAGCAG GCCTGCACCA CAGCCTCTCA 
CACTCACTTC TTGCCGTGGC CCAGGCCCCT GAGGCCACTG TCCTGGAGGC AGAGACCCGC 
AGGCTGGATG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGTCCT GCTACACCCC 
AGCCCCAGCA CTACCCAAGC CGCCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305148.1
CDS	2126..7558
Translation	MEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	NM_001318219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318219.1

ORF Insert Sequence:

ATGGAGGCCG CAGAGCAGGC GAGAAATGCT TTGCAGGTCG ACCTGGCGGA GGCAGAGAAG 
AGGAGGGAAG CCCTGTGGGA AAAGAACACT CACCTGGAGG CTCAGCTGCA GAAAGCTGAG 
GAGGCTGGGG CTGAGCTGCA GGCAGATCTC AGGGACATCC AAGAAGAGAA GGAAGAAATT 
CAAAAGAAAC TAAGTGAGTC ACGTCACCAG CAGGAGGCAG CCACGACTCA GCTGGAGCAG 
CTACATCAGG AGGCAAAGCG ACAGGAAGAA GTGCTTGCCA GGGCAGTCCA GGAGAAGGAG 
GCCCTAGTAC GAGAGAAAGC GGCTCTAGAG GTGCGGCTGC AGGCCGTGGA GCGTGACCGG 
CAGGACCTCG CTGAACAACT ACAGGGGCTC AGCTCAGCCA AGGAGCTACT GGAGAGCAGT 
CTGTTTGAAG CCCAACAACA AAATTCTGTG ATAGAGGTCA CCAAGGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACTCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAG GTGCCTGAAG 
CTGGAACTGG ACACTGAACG GAGTCAGGCA GAGCAGGAGC GGGATGCTGC AGCCAGACAG 
CTGGCCCAGG CTGAGCAAGA AGGGAAGACT GCCTTGGAGC AGCAGAAGGC AGCCCATGAG 
AAAGAGGTGA ACCAGCTCCG GGAGAAATGG GAGAAGGAGC GCTCCTGGCA CCAGCAGGAG 
CTGGCAAAGG CTCTGGAGAG CTTAGAAAGG GAAAAAATGG AGCTGGAAAT GAGGCTAAAG 
GAGCAGCAGA CAGAAATGGA GGCCATCCAG GCCCAGAGGG AAGAAGAACG GACCCAGGCA 
GAGAGTGCCC TATGCCAGAT GCAGCTGGAA ACAGAGAAGG AGAGAGTATC CCTCCTGGAG 
ACACTGCTGC AGACGCAGAA GGAGCTAGCA GATGCCAGCC AACAACTGGA ACGACTGAGG 
CAGGACATGA AAGTCCAGAA ATTAAAGGAG CAGGAGACCA CTGGGATACT ACAGACCCAG 
CTCCAGGAGG CTCAACGGGA GCTGAAGGAG GCAGCCCGGC AGCACAGAGA TGACCTTGCT 
GCCCTCCAAG AAGAGAGCAG CTCCCTGCTG CAGGATAAGA TGGACCTGCA GAAGCAGGTG 
GAGGACTTGA AGTCTCAGCT GGTGGCCCAG GATGACTCCC AGAGGCTGGT GGAGCAGGAG 
GTTCAGGAGA AGCTGAGAGA GACCCAGGAG TATAACCGAA TTCAGAAGGA GCTGGAGAGA 
GAGAAAGCCA GCCTGACTCT GTCACTGATG GAAAAGGAAC AGAGACTCCT TGTTTTACAA 
GAAGCTGACT CTATTCGACA ACAAGAGCTG AGTGCCCTGC GCCAGGACAT GCAGGAGGCC 
CAGGGAGAAC AGAAAGAGCT CAGTGCTCAG ATGGAATTAC TAAGGCAAGA GGTGAAGGAA 
AAGGAGGCTG ACTTTCTGGC CCAGGAAGCA CAGCTGCTGG AGGAGCTGGA GGCGTCTCAT 
ATCACGGAGC AGCAGCTGCG AGCCTCCTTG TGGGCCCAGG AAGCCAAGGC AGCCCAACTA 
CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG CTAGAAGCGC TGGCCGCAGA GCAGCAGCCC 
GGGAACCAGG CCCAGGCCCA GGCCCAGCTG GCCAGCCTCT ACTCTGCCCT GCAGCAGGCC 
CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG CTGAGTGGTG GGGGAGACTC TGCTCCTTCC 
GTCTGGGGCC TTGAGCCAGA CCAGAATGGA GCTAGGAGCC TCTTTAAGAG AGGGCCCCTG 
CTGACTGCTC TCTCCGCTGA GGCAGTAGCA TCTGCCCTCC ACAAGCTTCA TCAAGACCTG 
TGGAAGACTC AACAGACCCG GGATGTTCTG AGGGATCAGG TCCAGAAACT GGAAGAGCGT 
CTAACTGATA CTGAGGCTGA GAAGAGCCAG GTCCACACAG AGTTGCAGGA TCTGCAGAGA 
CAGCTCTCCC AGAATCAGGA AGAGAAATCC AAGTGGGAAG GAAAGCAGAA CTCCCTAGAA 
TCTGAGCTGA TGGAACTACA TGAAACTATG GCATCCTTAC AGAGTCGCCT GCGGAGAGCA 
GAGCTACAGC GAATGGAAGC CCAGGGTGAG CGAGAGTTAC TTCAGGCAGC CAAGGAGAAC 
CTGACAGCCC AGGTGGAACA CCTGCAAGCA GCTGTCGTAG AAGCCAGGGC TCAGGCAAGT 
GCTGCTGGCA TCCTGGAAGA AGACCTGAGA ACGGCTCGCT CAGCACTGAA GCTGAAAAAT 
GAGGAAGTAG AGAGTGAGCG TGAGAGAGCC CAGGCTCTGC AAGAGCAGGG CGAACTGAAG 
GTGGCCCAAG GGAAGGCTCT GCAAGAGAAT TTGGCCCTCC TGACCCAGAC CCTAGCTGAA 
AGAGAAGAGG AGGTGGAGAC TCTGCGGGGA CAAATCCAGG AACTGGAGAA GCAACGGGAA 
ATGCAGAAGG CTGCTTTGGA ATTGCTGTCT CTGGACCTGA AGAAGAGGAA CCAAGAGGTA 
GATCTGCAGC AAGAACAGAT TCAGGAGCTA GAGAAGTGTA GGTCTGTTTT AGAGCATCTG 
CCCATGGCCG TCCAGGAGCG AGAGCAGAAG CTGACTGTGC AGAGGGAGCA GATCAGAGAG 
CTCGAGAAGG ATCGGGAGAC TCAGAGGAAC GTCTTGGAGC ATCAGCTTCT AGAACTTGAG 
AAGAAAGACC AAATGATTGA GTCCCAGAGA GGACAGGTTC AGGACCTGAA AAAGCAGTTG 
GTTACTCTGG AATGCCTGGC CCTGGAACTG GAGGAAAACC ATCACAAGAT GGAGTGCCAG 
CAAAAACTGA TCAAGGAGCT GGAGGGCCAG AGGGAAACCC AGAGAGTGGC TTTGACCCAC 
CTTACGCTGG ACCTAGAAGA AAGGAGCCAG GAGCTGCAGG CACAAAGCAG CCAGATCCAT 
GACCTGGAGA GCCACAGCAC CGTTCTGGCA AGAGAGCTGC AGGAGAGGGA CCAGGAGGTG 
AAGTCTCAGC GAGAACAGAT CGAGGAGCTG CAGAGGCAGA AAGAGCATCT GACTCAGGAT 
CTCGAGAGGA GAGACCAGGA GCTGATGCTG CAGAAGGAGA GGATTCAGGT TCTCGAGGAT 
CAGAGGACCC GGCAGACCAA GATCCTGGAG GAGGACCTGG AACAGATCAA GCTGTCCTTG 
AGAGAGCGAG GCCGGGAGCT GACCACTCAG AGGCAGCTGA TGCAGGAACG GGCAGAGGAA 
GGGAAGGGCC CAAGTAAAGC ACAGCGCGGG AGCCTAGAGC ACATGAAGCT GATCCTGCGT 
GATAAGGAGA AGGAGGTGGA ATGTCAGCAG GAGCATATCC ATGAACTCCA GGAGCTCAAA 
GACCAGCTGG AGCAGCAGCT CCAGGGCCTG CACAGGAAGG TAGGTGAGAC CAGCCTCCTC 
CTGTCCCAGC GAGAGCAGGA AATAGTGGTC CTGCAGCAGC AACTGCAGGA AGCCAGGGAA 
CAAGGGGAGC TGAAGGAGCA GTCACTTCAG AGTCAACTGG ATGAGGCCCA GAGAGCCCTA 
GCCCAGAGGG ACCAGGAACT GGAGGCTCTG CAGCAAGAAC AGCAGCAGGC CCAGGGACAG 
GAGGAGAGGG TGAAGGAAAA GGCAGACGCC CTCCAGGGAG CTCTGGAGCA AGCCCATATG 
ACACTGAAGG AGCGTCATGG AGAGCTTCAG GACCACAAGG AACAGGCACG AAGGCTGGAG 
GAAGAGCTGG CAGTGGAGGG ACGGCGGGTC CAGGCCCTGG AGGAGGTGCT GGGAGACCTA 
AGGGCTGAGT CTCGGGAACA GGAGAAAGCT CTGTTGGCCC TCCAGCAGCA GTGTGCTGAG 
CAGGCACAGG AGCATGAGGT GGAGACCAGG GCCCTGCAGG ACAGCTGGCT GCAGGCCCAG 
GCAGTGCTCA AGGAACGGGA CCAGGAGCTG GAAGCTCTGC GGGCAGAAAG TCAGTCCTCC 
CGGCATCAGG AGGAGGCTGC CCGGGCCCGG GCTGAGGCTC TGCAGGAGGC CCTTGGCAAG 
GCTCATGCTG CCCTGCAGGG GAAAGAGCAG CATCTCCTCG AGCAGGCAGA ATTGAGCCGC 
AGTCTGGAGG CCAGCACTGC AACCCTGCAA GCCTCCCTGG ATGCCTGCCA GGCACACAGT 
CGGCAGCTGG AGGAGGCTCT GAGGATACAA GAAGGTGAGA TCCAGGACCA GGATCTCCGA 
TACCAGGAGG ATGTGCAGCA GCTGCAGCAG GCACTTGCCC AGAGGGATGA AGAGCTGAGA 
CATCAGCAGG AACGGGAGCA GCTGCTGGAG AAGTCTCTGG CCCAGAGGGT CCAAGAGAAT 
ATGATCCAAG AGAAGCAGAA TCTGGGGCAA GAGAGAGAAG AGGAGGAGAT AAGGGGCCTT 
CATCAGAGTG TAAGGGAGCT ACAGCTGACT CTAGCCCAAA AGGAACAGGA GATTCTGGAG 
CTGAGGGAGA CCCAGCAAAG GAACAACCTG GAAGCCTTAC CCCACAGCCA CAAAACCTCC 
CCAATGGAGG AACAATCTCT AAAACTTGAT TCTTTAGAGC CCAGGCTGCA GCGGGAGCTG 
GAGCGGCTAC AGGCAGCCCT GAGACAGACA GAAGCCAGGG AGATTGAGTG GAGGGAGAAG 
GCCCAGGACT TGGCACTCTC CCTAGCGCAG ACCAAGGCCA GTGTCAGCAG TCTGCAGGAG 
GTAGCCATGT TCCTACAAGC CTCTGTCCTG GAGCGGGACT CAGAACAGCA AAGGCTGCAG 
GATGAACTGG AGCTCACCAG ACGGGCTCTG GAGAAGGAGC GGCTACACAG CCCAGGTGCA 
ACCAGCACAG CAGAACTGGG GTCCAGAGGG GAGCAGGGTG TGCAGCTGGG AGAGGTCTCA 
GGAGTGGAGG CTGAGCCTAG TCCTGATGGA ATGGAGAAGC AGTCATGGAG ACAAAGGCTT 
GAACACCTGC AGCAAGCAGT GGCCCGGCTG GAGATTGACA GGAGCAGGCT GCAGCGCCAC 
AATGTCCAGC TGCGGAGTAC CTTGGAGCAG GTGGAGCGAG AACGGAGGAA GCTGAAGAGG 
GAGGCCATGC GTGCGGCCCA GGCAGGGTCC CTAGAGATCA GCAAGGCCAC GGCTTCTTCA 
CCCACACAGC AGGATGGGAG AGGACAGAAG AACTCAGATG CCAAGTGTGT GGCTGAACTG 
CAGAAAGAGG TGGTCCTGCT GCAAGCTCAG CTGACTTTGG AGCGGAAGCA GAAGCAGGAC 
TACATCACCC GCTCAGCACA GACCAGCCGT GAGCTAGCAG GCCTGCACCA CAGCCTCTCA 
CACTCACTTC TTGCCGTGGC CCAGGCCCCT GAGGCCACTG TCCTGGAGGC AGAGACCCGC 
AGGCTGGATG AGTCCCTGAC TCAAAGTCTG ACATCCCCAG GGCCAGTCCT GCTACACCCC 
AGCCCCAGCA CTACCCAAGC CGCCTCCAGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( NM_007186.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_009117.2 Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.

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CloneID	OHu23771
Clone ID Related Accession (Same CDS sequence)	NM_007186.5
Accession Version	NM_007186.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF049105.1, FO393401.1 and AW083819.1. This sequence is a reference standard in the RefSeqGene project. On Dec 30, 2015 this sequence version replaced NM_007186.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF049105.1, AF022655.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTGTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009117.2
CDS	648..7976
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	NM_007186.5
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007186.5

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTGTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCCAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( NM_007186.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_009117.2 Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	NM_007186.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FO393401.1. On Nov 23, 2018 this sequence version replaced NM_007186.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF049105.1, AF022655.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397527.6/ ENSP00000380661.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009117.2
CDS	452..7780
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	NM_007186.6
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007186.6

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723691.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723754.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723754.1
CDS	614..7942
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723691.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723691.1

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723690.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723753.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723690.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723753.1
CDS	398..7726
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723690.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723690.3

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723690.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723753.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006723690.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723753.1
CDS	399..7727
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723690.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723690.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723692.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723755.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006723692.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723755.1
CDS	325..7653
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723692.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723692.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723692.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723755.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723692.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723755.1
CDS	324..7652
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723692.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723692.3

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005260262.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260319.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_005260262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260262.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260319.1
CDS	401..7729
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_005260262.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260262.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723693.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723756.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723756.1
CDS	308..7636
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723693.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723693.3

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723693.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723756.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OHu52874
Clone ID Related Accession (Same CDS sequence)	NM_007186.6 , XM_006723691.1 , XM_006723690.3 , XM_006723690.4 , XM_006723692.4 , XM_006723692.3 , XM_005260262.4 , XM_006723693.3 , XM_006723693.4
Accession Version	XM_006723693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006723693.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723756.1
CDS	309..7637
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723693.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723693.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGATG AACTGGAGCT CACCAGACGG 
GCTCTGGAGA AGGAGCGGCT ACACAGCCCA GGTGCAACCA GCACAGCAGA ACTGGGGTCC 
AGAGGGGAGC AGGGTGTGCA GCTGGGAGAG GTCTCAGGAG TGGAGGCTGA GCCTAGTCCT 
GATGGAATGG AGAAGCAGTC ATGGAGACAA AGGCTTGAAC ACCTGCAGCA AGCAGTGGCC 
CGGCTGGAGA TTGACAGGAG CAGGCTGCAG CGCCACAATG TCCAGCTGCG GAGTACCTTG 
GAGCAGGTGG AGCGAGAACG GAGGAAGCTG AAGAGGGAGG CCATGCGTGC GGCCCAGGCA 
GGGTCCCTAG AGATCAGCAA GGCCACGGCT TCTTCACCCA CACAGCAGGA TGGGAGAGGA 
CAGAAGAACT CAGATGCCAA GTGTGTGGCT GAACTGCAGA AAGAGGTGGT CCTGCTGCAA 
GCTCAGCTGA CTTTGGAGCG GAAGCAGAAG CAGGACTACA TCACCCGCTC AGCACAGACC 
AGCCGTGAGC TAGCAGGCCT GCACCACAGC CTCTCACACT CACTTCTTGC CGTGGCCCAG 
GCCCCTGAGG CCACTGTCCT GGAGGCAGAG ACCCGCAGGC TGGATGAGTC CCTGACTCAA 
AGTCTGACAT CCCCAGGGCC AGTCCTGCTA CACCCCAGCC CCAGCACTAC CCAAGCCGCC 
TCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_006723694.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723757.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

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CloneID	OHu52875
Clone ID Related Accession (Same CDS sequence)	XM_006723694.3
Accession Version	XM_006723694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGTCT CAGGAGTGGA GGCTGAGCCT 
AGTCCTGATG GAATGGAGAA GCAGTCATGG AGACAAAGGC TTGAACACCT GCAGCAAGCA 
GTGGCCCGGC TGGAGATTGA CAGGAGCAGG CTGCAGCGCC ACAATGTCCA GCTGCGGAGT 
ACCTTGGAGC AGGTGGAGCG AGAACGGAGG AAGCTGAAGA GGGAGGCCAT GCGTGCGGCC 
CAGGCAGGGT CCCTAGAGAT CAGCAAGGCC ACGGCTTCTT CACCCACACA GCAGGATGGG 
AGAGGACAGA AGAACTCAGA TGCCAAGTGT GTGGCTGAAC TGCAGAAAGA GGTGGTCCTG 
CTGCAAGCTC AGCTGACTTT GGAGCGGAAG CAGAAGCAGG ACTACATCAC CCGCTCAGCA 
CAGACCAGCC GTGAGCTAGC AGGCCTGCAC CACAGCCTCT CACACTCACT TCTTGCCGTG 
GCCCAGGCCC CTGAGGCCAC TGTCCTGGAG GCAGAGACCC GCAGGCTGGA TGAGTCCCTG 
ACTCAAAGTC TGACATCCCC AGGGCCAGTC CTGCTACACC CCAGCCCCAG CACTACCCAA 
GCCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723757.1
CDS	401..7615
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_006723694.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723694.3

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTTAGAGG AGGAGAACCA GTCTGTGTGC AGCAGAATGG AGGCCGCAGA GCAGGCGAGA 
AATGCTTTGC AGGTCGACCT GGCGGAGGCA GAGAAGAGGA GGGAAGCCCT GTGGGAAAAG 
AACACTCACC TGGAGGCTCA GCTGCAGAAA GCTGAGGAGG CTGGGGCTGA GCTGCAGGCA 
GATCTCAGGG ACATCCAAGA AGAGAAGGAA GAAATTCAAA AGAAACTAAG TGAGTCACGT 
CACCAGCAGG AGGCAGCCAC GACTCAGCTG GAGCAGCTAC ATCAGGAGGC AAAGCGACAG 
GAAGAAGTGC TTGCCAGGGC AGTCCAGGAG AAGGAGGCCC TAGTACGAGA GAAAGCGGCT 
CTAGAGGTGC GGCTGCAGGC CGTGGAGCGT GACCGGCAGG ACCTCGCTGA ACAACTACAG 
GGGCTCAGCT CAGCCAAGGA GCTACTGGAG AGCAGTCTGT TTGAAGCCCA ACAACAAAAT 
TCTGTGATAG AGGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTGT CACTCAAGCC 
AAGGAAGTAA TCCAAGGGGA AGTGAGGTGC CTGAAGCTGG AACTGGACAC TGAACGGAGT 
CAGGCAGAGC AGGAGCGGGA TGCTGCAGCC AGACAGCTGG CCCAGGCTGA GCAAGAAGGG 
AAGACTGCCT TGGAGCAGCA GAAGGCAGCC CATGAGAAAG AGGTGAACCA GCTCCGGGAG 
AAATGGGAGA AGGAGCGCTC CTGGCACCAG CAGGAGCTGG CAAAGGCTCT GGAGAGCTTA 
GAAAGGGAAA AAATGGAGCT GGAAATGAGG CTAAAGGAGC AGCAGACAGA AATGGAGGCC 
ATCCAGGCCC AGAGGGAAGA AGAACGGACC CAGGCAGAGA GTGCCCTATG CCAGATGCAG 
CTGGAAACAG AGAAGGAGAG AGTATCCCTC CTGGAGACAC TGCTGCAGAC GCAGAAGGAG 
CTAGCAGATG CCAGCCAACA ACTGGAACGA CTGAGGCAGG ACATGAAAGT CCAGAAATTA 
AAGGAGCAGG AGACCACTGG GATACTACAG ACCCAGCTCC AGGAGGCTCA ACGGGAGCTG 
AAGGAGGCAG CCCGGCAGCA CAGAGATGAC CTTGCTGCCC TCCAAGAAGA GAGCAGCTCC 
CTGCTGCAGG ATAAGATGGA CCTGCAGAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTG 
GCCCAGGATG ACTCCCAGAG GCTGGTGGAG CAGGAGGTTC AGGAGAAGCT GAGAGAGACC 
CAGGAGTATA ACCGAATTCA GAAGGAGCTG GAGAGAGAGA AAGCCAGCCT GACTCTCTCA 
CTGATGGAAA AGGAACAGAG ACTCCTTGTT TTACAAGAAG CTGACTCTAT TCGACAACAA 
GAGCTGAGTG CCCTGCGCCA GGACATGCAG GAGGCCCAGG GAGAACAGAA AGAGCTCAGT 
GCTCAGATGG AATTACTAAG GCAAGAGGTG AAGGAAAAGG AGGCTGACTT TCTGGCCCAG 
GAAGCACAGC TGCTGGAGGA GCTGGAGGCG TCTCATATCA CGGAGCAGCA GCTGCGAGCC 
TCCTTGTGGG CCCAGGAAGC CAAGGCAGCC CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTAG AAGCGCTGGC CGCAGAGCAG CAGCCCGGGA ACCAGGCCCA GGCCCAGGCC 
CAGCTGGCCA GCCTCTACTC TGCCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGTGGGGG AGACTCTGCT CCTTCCGTCT GGGGCCTTGA GCCAGACCAG 
AATGGAGCTA GGAGCCTCTT TAAGAGAGGG CCCCTGCTGA CTGCTCTCTC CGCTGAGGCA 
GTAGCATCTG CCCTCCACAA GCTTCATCAA GACCTGTGGA AGACTCAACA GACCCGGGAT 
GTTCTGAGGG ATCAGGTCCA GAAACTGGAA GAGCGTCTAA CTGATACTGA GGCTGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGATCTG CAGAGACAGC TCTCCCAGAA TCAGGAAGAG 
AAATCTAAGT GGGAAGGAAA GCAGAACTCC CTAGAATCTG AGCTGATGGA ACTACATGAA 
ACTATGGCAT CCTTACAGAG TCGCCTGCGG AGAGCAGAGC TACAGCGAAT GGAAGCCCAG 
GGTGAGCGAG AGTTACTTCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTG 
CAAGCAGCTG TCGTAGAAGC CAGGGCTCAG GCAAGTGCTG CTGGCATCCT GGAAGAAGAC 
CTGAGAACGG CTCGCTCAGC ACTGAAGCTG AAAAATGAGG AAGTAGAGAG TGAGCGTGAG 
AGAGCCCAGG CTCTGCAAGA GCAGGGCGAA CTGAAGGTGG CCCAAGGGAA GGCTCTGCAA 
GAGAATTTGG CCCTCCTGAC CCAGACCCTA GCTGAAAGAG AAGAGGAGGT GGAGACTCTG 
CGGGGACAAA TCCAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGC TTTGGAATTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCAA GAGGTAGATC TGCAGCAAGA ACAGATTCAG 
GAGCTAGAGA AGTGTAGGTC TGTTTTAGAG CATCTGCCCA TGGCCGTCCA GGAGCGAGAG 
CAGAAGCTGA CTGTGCAGAG GGAGCAGATC AGAGAGCTCG AGAAGGATCG GGAGACTCAG 
AGGAACGTCT TGGAGCATCA GCTTCTAGAA CTTGAGAAGA AAGACCAAAT GATTGAGTCC 
CAGAGAGGAC AGGTTCAGGA CCTGAAAAAG CAGTTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTGGAGG AAAACCATCA CAAGATGGAG TGCCAGCAAA AACTGATCAA GGAGCTGGAG 
GGCCAGAGGG AAACCCAGAG AGTGGCTTTG ACCCACCTTA CGCTGGACCT AGAAGAAAGG 
AGCCAGGAGC TGCAGGCACA AAGCAGCCAG ATCCATGACC TGGAGAGCCA CAGCACCGTT 
CTGGCAAGAG AGCTGCAGGA GAGGGACCAG GAGGTGAAGT CTCAGCGAGA ACAGATCGAG 
GAGCTGCAGA GGCAGAAAGA GCATCTGACT CAGGATCTCG AGAGGAGAGA CCAGGAGCTG 
ATGCTGCAGA AGGAGAGGAT TCAGGTTCTC GAGGATCAGA GGACCCGGCA GACCAAGATC 
CTGGAGGAGG ACCTGGAACA GATCAAGCTG TCCTTGAGAG AGCGAGGCCG GGAGCTGACC 
ACTCAGAGGC AGCTGATGCA GGAACGGGCA GAGGAAGGGA AGGGCCCAAG TAAAGCACAG 
CGCGGGAGCC TAGAGCACAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGT 
CAGCAGGAGC ATATCCATGA ACTCCAGGAG CTCAAAGACC AGCTGGAGCA GCAGCTCCAG 
GGCCTGCACA GGAAGGTAGG TGAGACCAGC CTCCTCCTGT CCCAGCGAGA GCAGGAAATA 
GTGGTCCTGC AGCAGCAACT GCAGGAAGCC AGGGAACAAG GGGAGCTGAA GGAGCAGTCA 
CTTCAGAGTC AACTGGATGA GGCCCAGAGA GCCCTAGCCC AGAGGGACCA GGAACTGGAG 
GCTCTGCAGC AAGAACAGCA GCAGGCCCAG GGACAGGAGG AGAGGGTGAA GGAAAAGGCA 
GACGCCCTCC AGGGAGCTCT GGAGCAAGCC CATATGACAC TGAAGGAGCG TCATGGAGAG 
CTTCAGGACC ACAAGGAACA GGCACGAAGG CTGGAGGAAG AGCTGGCAGT GGAGGGACGG 
CGGGTCCAGG CCCTGGAGGA GGTGCTGGGA GACCTAAGGG CTGAGTCTCG GGAACAGGAG 
AAAGCTCTGT TGGCCCTCCA GCAGCAGTGT GCTGAGCAGG CACAGGAGCA TGAGGTGGAG 
ACCAGGGCCC TGCAGGACAG CTGGCTGCAG GCCCAGGCAG TGCTCAAGGA ACGGGACCAG 
GAGCTGGAAG CTCTGCGGGC AGAAAGTCAG TCCTCCCGGC ATCAGGAGGA GGCTGCCCGG 
GCCCGGGCTG AGGCTCTGCA GGAGGCCCTT GGCAAGGCTC ATGCTGCCCT GCAGGGGAAA 
GAGCAGCATC TCCTCGAGCA GGCAGAATTG AGCCGCAGTC TGGAGGCCAG CACTGCAACC 
CTGCAAGCCT CCCTGGATGC CTGCCAGGCA CACAGTCGGC AGCTGGAGGA GGCTCTGAGG 
ATACAAGAAG GTGAGATCCA GGACCAGGAT CTCCGATACC AGGAGGATGT GCAGCAGCTG 
CAGCAGGCAC TTGCCCAGAG GGATGAAGAG CTGAGACATC AGCAGGAACG GGAGCAGCTG 
CTGGAGAAGT CTCTGGCCCA GAGGGTCCAA GAGAATATGA TCCAAGAGAA GCAGAATCTG 
GGGCAAGAGA GAGAAGAGGA GGAGATAAGG GGCCTTCATC AGAGTGTAAG GGAGCTACAG 
CTGACTCTAG CCCAAAAGGA ACAGGAGATT CTGGAGCTGA GGGAGACCCA GCAAAGGAAC 
AACCTGGAAG CCTTACCCCA CAGCCACAAA ACCTCCCCAA TGGAGGAACA ATCTCTAAAA 
CTTGATTCTT TAGAGCCCAG GCTGCAGCGG GAGCTGGAGC GGCTACAGGC AGCCCTGAGA 
CAGACAGAAG CCAGGGAGAT TGAGTGGAGG GAGAAGGCCC AGGACTTGGC ACTCTCCCTA 
GCGCAGACCA AGGCCAGTGT CAGCAGTCTG CAGGAGGTAG CCATGTTCCT ACAAGCCTCT 
GTCCTGGAGC GGGACTCAGA ACAGCAAAGG CTGCAGGTCT CAGGAGTGGA GGCTGAGCCT 
AGTCCTGATG GAATGGAGAA GCAGTCATGG AGACAAAGGC TTGAACACCT GCAGCAAGCA 
GTGGCCCGGC TGGAGATTGA CAGGAGCAGG CTGCAGCGCC ACAATGTCCA GCTGCGGAGT 
ACCTTGGAGC AGGTGGAGCG AGAACGGAGG AAGCTGAAGA GGGAGGCCAT GCGTGCGGCC 
CAGGCAGGGT CCCTAGAGAT CAGCAAGGCC ACGGCTTCTT CACCCACACA GCAGGATGGG 
AGAGGACAGA AGAACTCAGA TGCCAAGTGT GTGGCTGAAC TGCAGAAAGA GGTGGTCCTG 
CTGCAAGCTC AGCTGACTTT GGAGCGGAAG CAGAAGCAGG ACTACATCAC CCGCTCAGCA 
CAGACCAGCC GTGAGCTAGC AGGCCTGCAC CACAGCCTCT CACACTCACT TCTTGCCGTG 
GCCCAGGCCC CTGAGGCCAC TGTCCTGGAG GCAGAGACCC GCAGGCTGGA TGAGTCCCTG 
ACTCAAAGTC TGACATCCCC AGGGCCAGTC CTGCTACACC CCAGCCCCAG CACTACCCAA 
GCCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005260264.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260321.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

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CloneID	OHu52878
Clone ID Related Accession (Same CDS sequence)	XM_005260264.4
Accession Version	XM_005260264.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTCACGTC ACCAGCAGGA GGCAGCCACG ACTCAGCTGG AGCAGCTACA TCAGGAGGCA 
AAGCGACAGG AAGAAGTGCT TGCCAGGGCA GTCCAGGAGA AGGAGGCCCT AGTACGAGAG 
AAAGCGGCTC TAGAGGTGCG GCTGCAGGCC GTGGAGCGTG ACCGGCAGGA CCTCGCTGAA 
CAACTACAGG GGCTCAGCTC AGCCAAGGAG CTACTGGAGA GCAGTCTGTT TGAAGCCCAA 
CAACAAAATT CTGTGATAGA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTGTC 
ACTCAAGCCA AGGAAGTAAT CCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACACT 
GAACGGAGTC AGGCAGAGCA GGAGCGGGAT GCTGCAGCCA GACAGCTGGC CCAGGCTGAG 
CAAGAAGGGA AGACTGCCTT GGAGCAGCAG AAGGCAGCCC ATGAGAAAGA GGTGAACCAG 
CTCCGGGAGA AATGGGAGAA GGAGCGCTCC TGGCACCAGC AGGAGCTGGC AAAGGCTCTG 
GAGAGCTTAG AAAGGGAAAA AATGGAGCTG GAAATGAGGC TAAAGGAGCA GCAGACAGAA 
ATGGAGGCCA TCCAGGCCCA GAGGGAAGAA GAACGGACCC AGGCAGAGAG TGCCCTATGC 
CAGATGCAGC TGGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGCAGACG 
CAGAAGGAGC TAGCAGATGC CAGCCAACAA CTGGAACGAC TGAGGCAGGA CATGAAAGTC 
CAGAAATTAA AGGAGCAGGA GACCACTGGG ATACTACAGA CCCAGCTCCA GGAGGCTCAA 
CGGGAGCTGA AGGAGGCAGC CCGGCAGCAC AGAGATGACC TTGCTGCCCT CCAAGAAGAG 
AGCAGCTCCC TGCTGCAGGA TAAGATGGAC CTGCAGAAGC AGGTGGAGGA CTTGAAGTCT 
CAGCTGGTGG CCCAGGATGA CTCCCAGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGAGAGACCC AGGAGTATAA CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ACTCTCTCAC TGATGGAAAA GGAACAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTATT 
CGACAACAAG AGCTGAGTGC CCTGCGCCAG GACATGCAGG AGGCCCAGGG AGAACAGAAA 
GAGCTCAGTG CTCAGATGGA ATTACTAAGG CAAGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCCCAGG AAGCACAGCT GCTGGAGGAG CTGGAGGCGT CTCATATCAC GGAGCAGCAG 
CTGCGAGCCT CCTTGTGGGC CCAGGAAGCC AAGGCAGCCC AACTACAGCT GCGACTGCGC 
AGCACAGAGA GCCAGCTAGA AGCGCTGGCC GCAGAGCAGC AGCCCGGGAA CCAGGCCCAG 
GCCCAGGCCC AGCTGGCCAG CCTCTACTCT GCCCTGCAGC AGGCCCTGGG GTCTGTTTGT 
GAGAGCAGGC CTGAGCTGAG TGGTGGGGGA GACTCTGCTC CTTCCGTCTG GGGCCTTGAG 
CCAGACCAGA ATGGAGCTAG GAGCCTCTTT AAGAGAGGGC CCCTGCTGAC TGCTCTCTCC 
GCTGAGGCAG TAGCATCTGC CCTCCACAAG CTTCATCAAG ACCTGTGGAA GACTCAACAG 
ACCCGGGATG TTCTGAGGGA TCAGGTCCAG AAACTGGAAG AGCGTCTAAC TGATACTGAG 
GCTGAGAAGA GCCAGGTCCA CACAGAGTTG CAGGATCTGC AGAGACAGCT CTCCCAGAAT 
CAGGAAGAGA AATCTAAGTG GGAAGGAAAG CAGAACTCCC TAGAATCTGA GCTGATGGAA 
CTACATGAAA CTATGGCATC CTTACAGAGT CGCCTGCGGA GAGCAGAGCT ACAGCGAATG 
GAAGCCCAGG GTGAGCGAGA GTTACTTCAG GCAGCCAAGG AGAACCTGAC AGCCCAGGTG 
GAACACCTGC AAGCAGCTGT CGTAGAAGCC AGGGCTCAGG CAAGTGCTGC TGGCATCCTG 
GAAGAAGACC TGAGAACGGC TCGCTCAGCA CTGAAGCTGA AAAATGAGGA AGTAGAGAGT 
GAGCGTGAGA GAGCCCAGGC TCTGCAAGAG CAGGGCGAAC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAAG AGAATTTGGC CCTCCTGACC CAGACCCTAG CTGAAAGAGA AGAGGAGGTG 
GAGACTCTGC GGGGACAAAT CCAGGAACTG GAGAAGCAAC GGGAAATGCA GAAGGCTGCT 
TTGGAATTGC TGTCTCTGGA CCTGAAGAAG AGGAACCAAG AGGTAGATCT GCAGCAAGAA 
CAGATTCAGG AGCTAGAGAA GTGTAGGTCT GTTTTAGAGC ATCTGCCCAT GGCCGTCCAG 
GAGCGAGAGC AGAAGCTGAC TGTGCAGAGG GAGCAGATCA GAGAGCTCGA GAAGGATCGG 
GAGACTCAGA GGAACGTCTT GGAGCATCAG CTTCTAGAAC TTGAGAAGAA AGACCAAATG 
ATTGAGTCCC AGAGAGGACA GGTTCAGGAC CTGAAAAAGC AGTTGGTTAC TCTGGAATGC 
CTGGCCCTGG AACTGGAGGA AAACCATCAC AAGATGGAGT GCCAGCAAAA ACTGATCAAG 
GAGCTGGAGG GCCAGAGGGA AACCCAGAGA GTGGCTTTGA CCCACCTTAC GCTGGACCTA 
GAAGAAAGGA GCCAGGAGCT GCAGGCACAA AGCAGCCAGA TCCATGACCT GGAGAGCCAC 
AGCACCGTTC TGGCAAGAGA GCTGCAGGAG AGGGACCAGG AGGTGAAGTC TCAGCGAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAAGAG CATCTGACTC AGGATCTCGA GAGGAGAGAC 
CAGGAGCTGA TGCTGCAGAA GGAGAGGATT CAGGTTCTCG AGGATCAGAG GACCCGGCAG 
ACCAAGATCC TGGAGGAGGA CCTGGAACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGACCA CTCAGAGGCA GCTGATGCAG GAACGGGCAG AGGAAGGGAA GGGCCCAAGT 
AAAGCACAGC GCGGGAGCCT AGAGCACATG AAGCTGATCC TGCGTGATAA GGAGAAGGAG 
GTGGAATGTC AGCAGGAGCA TATCCATGAA CTCCAGGAGC TCAAAGACCA GCTGGAGCAG 
CAGCTCCAGG GCCTGCACAG GAAGGTAGGT GAGACCAGCC TCCTCCTGTC CCAGCGAGAG 
CAGGAAATAG TGGTCCTGCA GCAGCAACTG CAGGAAGCCA GGGAACAAGG GGAGCTGAAG 
GAGCAGTCAC TTCAGAGTCA ACTGGATGAG GCCCAGAGAG CCCTAGCCCA GAGGGACCAG 
GAACTGGAGG CTCTGCAGCA AGAACAGCAG CAGGCCCAGG GACAGGAGGA GAGGGTGAAG 
GAAAAGGCAG ACGCCCTCCA GGGAGCTCTG GAGCAAGCCC ATATGACACT GAAGGAGCGT 
CATGGAGAGC TTCAGGACCA CAAGGAACAG GCACGAAGGC TGGAGGAAGA GCTGGCAGTG 
GAGGGACGGC GGGTCCAGGC CCTGGAGGAG GTGCTGGGAG ACCTAAGGGC TGAGTCTCGG 
GAACAGGAGA AAGCTCTGTT GGCCCTCCAG CAGCAGTGTG CTGAGCAGGC ACAGGAGCAT 
GAGGTGGAGA CCAGGGCCCT GCAGGACAGC TGGCTGCAGG CCCAGGCAGT GCTCAAGGAA 
CGGGACCAGG AGCTGGAAGC TCTGCGGGCA GAAAGTCAGT CCTCCCGGCA TCAGGAGGAG 
GCTGCCCGGG CCCGGGCTGA GGCTCTGCAG GAGGCCCTTG GCAAGGCTCA TGCTGCCCTG 
CAGGGGAAAG AGCAGCATCT CCTCGAGCAG GCAGAATTGA GCCGCAGTCT GGAGGCCAGC 
ACTGCAACCC TGCAAGCCTC CCTGGATGCC TGCCAGGCAC ACAGTCGGCA GCTGGAGGAG 
GCTCTGAGGA TACAAGAAGG TGAGATCCAG GACCAGGATC TCCGATACCA GGAGGATGTG 
CAGCAGCTGC AGCAGGCACT TGCCCAGAGG GATGAAGAGC TGAGACATCA GCAGGAACGG 
GAGCAGCTGC TGGAGAAGTC TCTGGCCCAG AGGGTCCAAG AGAATATGAT CCAAGAGAAG 
CAGAATCTGG GGCAAGAGAG AGAAGAGGAG GAGATAAGGG GCCTTCATCA GAGTGTAAGG 
GAGCTACAGC TGACTCTAGC CCAAAAGGAA CAGGAGATTC TGGAGCTGAG GGAGACCCAG 
CAAAGGAACA ACCTGGAAGC CTTACCCCAC AGCCACAAAA CCTCCCCAAT GGAGGAACAA 
TCTCTAAAAC TTGATTCTTT AGAGCCCAGG CTGCAGCGGG AGCTGGAGCG GCTACAGGCA 
GCCCTGAGAC AGACAGAAGC CAGGGAGATT GAGTGGAGGG AGAAGGCCCA GGACTTGGCA 
CTCTCCCTAG CGCAGACCAA GGCCAGTGTC AGCAGTCTGC AGGAGGTAGC CATGTTCCTA 
CAAGCCTCTG TCCTGGAGCG GGACTCAGAA CAGCAAAGGC TGCAGGATGA ACTGGAGCTC 
ACCAGACGGG CTCTGGAGAA GGAGCGGCTA CACAGCCCAG GTGCAACCAG CACAGCAGAA 
CTGGGGTCCA GAGGGGAGCA GGGTGTGCAG CTGGGAGAGG TCTCAGGAGT GGAGGCTGAG 
CCTAGTCCTG ATGGAATGGA GAAGCAGTCA TGGAGACAAA GGCTTGAACA CCTGCAGCAA 
GCAGTGGCCC GGCTGGAGAT TGACAGGAGC AGGCTGCAGC GCCACAATGT CCAGCTGCGG 
AGTACCTTGG AGCAGGTGGA GCGAGAACGG AGGAAGCTGA AGAGGGAGGC CATGCGTGCG 
GCCCAGGCAG GGTCCCTAGA GATCAGCAAG GCCACGGCTT CTTCACCCAC ACAGCAGGAT 
GGGAGAGGAC AGAAGAACTC AGATGCCAAG TGTGTGGCTG AACTGCAGAA AGAGGTGGTC 
CTGCTGCAAG CTCAGCTGAC TTTGGAGCGG AAGCAGAAGC AGGACTACAT CACCCGCTCA 
GCACAGACCA GCCGTGAGCT AGCAGGCCTG CACCACAGCC TCTCACACTC ACTTCTTGCC 
GTGGCCCAGG CCCCTGAGGC CACTGTCCTG GAGGCAGAGA CCCGCAGGCT GGATGAGTCC 
CTGACTCAAA GTCTGACATC CCCAGGGCCA GTCCTGCTAC ACCCCAGCCC CAGCACTACC 
CAAGCCGCCT CCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260321.1
CDS	401..7498
Translation	METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQSRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_005260264.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260264.4

ORF Insert Sequence:

ATGGAGACAA GAAGCCCTGG GTTGAACAAC ATGAAGCCCC AGTCACTGCA GCTGGTACTG 
GAAGAGCAGG TGCTGGCACT ACAGCAGCAG ATGGCAGAGA ATCAGGCAGC CTCCTGGCGG 
AAGCTGAAGA ACTCCCAGGA GGCCCAGCAG AGACAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGC TGCAGTACCG AAGCTGGTGC CAAGAGCTGG AGAAGCGGCT AGAAGCCACT 
GGAGGACCAA TCCCCCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA TGAGCTGCTG 
GTCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGACTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCCC TTAGGGAAGA TGTGGAAAAA 
CTGACAGTGG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TTGTGACATT CCGACGCCAC TTCCTGGAAA TGAAGTCAGC TACTGACAGA 
GATCTGATGG AGCTAAAAGC TGAGCATGTG AGGCTTTCAG GGTCTCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACAGTCTCGG GAACCCAACG GATCTGGAAG AATGGATGGG 
CGGGAGCCGG CCCAGCTGCT GCTGCTACTA GCCAAGACCC AGGAGCTGGA GAAGGAAGCC 
CATGAAAGGA GCCAGGAGTT AATACAGCTG AAGAGTCAAG GGGATCTGGA GAAGGCTGAA 
CTTCAGGACC GGGTGACCGA GCTCTCTGCT CTGTTGACCC AGTCTCAGAA GCAAAATGAA 
GATTATGAAA AGATGATAAA GGCTCTGAGA GAGACAGTGG AGATCCTGGA GACAAATCAC 
ACAGAATTAA TGGAACATGA AGCATCTCTT AGTAGGAATG CGCAAGAGGA GAAGTTGTCT 
TTACAGCAGG TGATCAAGGA TATAACCCAG GTCATGGTGG AAGAAGGGGA CAATATAGCC 
CAAGGCTCTG GTCATGAGAA CTCTTTGGAA TTGGACTCTA GTATCTTCTC CCAGTTTGAT 
TACCAGGATG CAGACAAGGC TCTTACTCTG GTGCGTTCAG TGCTGACTCG GAGACGCCAG 
GCTGTGCAGG ACCTAAGGCA GCAGCTTGCA GGCTGTCAAG AGGCTGTGAA CTTGTTGCAA 
CAGCAGCATG ATCAGTGGGA GGAAGAGGGC AAAGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACTGGGGAGC GGGACACTCT GGCAGGGCAG ACTGTGGACC TCCAGGGAGA GGTGGACTCT 
CTCAGCAAGG AGCGAGAGCT GCTGCAGAAG GCCAGGGAAG AGCTGCGGCA GCAGCTGGAG 
GTGCTAGAGC AGGAGGCATG GCGCCTGCGA AGGGTAAATG TGGAGCTTCA GCTGCAGGGG 
GACTCTGCCC AGGGCCAGAA GGAGGAACAG CAGGAGGAGC TGCACCTGGC TGTCCGGGAG 
AGGGAGCGTC TTCAGGAGAT GCTGATGGGC CTGGAAGCCA AACAGTCAGA ATCACTCAGT 
GAACTGATCA CTCTTCGGGA AGCCCTGGAG TCAAGTCACC TGGAAGGGGA GTTACTGAGG 
CAAGAGCAAA CGGAAGTGAC CGCAGCGCTG GCTAGGGCAG AGCAGTCAAT TGCAGAGCTG 
TCGAGTTCTG AAAACACCCT GAAGACAGAA GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCGTTA GATAAAGTTG GGCTGAACCA GCAGCTTCTC 
CAGTCACGTC ACCAGCAGGA GGCAGCCACG ACTCAGCTGG AGCAGCTACA TCAGGAGGCA 
AAGCGACAGG AAGAAGTGCT TGCCAGGGCA GTCCAGGAGA AGGAGGCCCT AGTACGAGAG 
AAAGCGGCTC TAGAGGTGCG GCTGCAGGCC GTGGAGCGTG ACCGGCAGGA CCTCGCTGAA 
CAACTACAGG GGCTCAGCTC AGCCAAGGAG CTACTGGAGA GCAGTCTGTT TGAAGCCCAA 
CAACAAAATT CTGTGATAGA GGTCACCAAG GGGCAGCTGG AGGTCCAGAT TCAAACTGTC 
ACTCAAGCCA AGGAAGTAAT CCAAGGGGAA GTGAGGTGCC TGAAGCTGGA ACTGGACACT 
GAACGGAGTC AGGCAGAGCA GGAGCGGGAT GCTGCAGCCA GACAGCTGGC CCAGGCTGAG 
CAAGAAGGGA AGACTGCCTT GGAGCAGCAG AAGGCAGCCC ATGAGAAAGA GGTGAACCAG 
CTCCGGGAGA AATGGGAGAA GGAGCGCTCC TGGCACCAGC AGGAGCTGGC AAAGGCTCTG 
GAGAGCTTAG AAAGGGAAAA AATGGAGCTG GAAATGAGGC TAAAGGAGCA GCAGACAGAA 
ATGGAGGCCA TCCAGGCCCA GAGGGAAGAA GAACGGACCC AGGCAGAGAG TGCCCTATGC 
CAGATGCAGC TGGAAACAGA GAAGGAGAGA GTATCCCTCC TGGAGACACT GCTGCAGACG 
CAGAAGGAGC TAGCAGATGC CAGCCAACAA CTGGAACGAC TGAGGCAGGA CATGAAAGTC 
CAGAAATTAA AGGAGCAGGA GACCACTGGG ATACTACAGA CCCAGCTCCA GGAGGCTCAA 
CGGGAGCTGA AGGAGGCAGC CCGGCAGCAC AGAGATGACC TTGCTGCCCT CCAAGAAGAG 
AGCAGCTCCC TGCTGCAGGA TAAGATGGAC CTGCAGAAGC AGGTGGAGGA CTTGAAGTCT 
CAGCTGGTGG CCCAGGATGA CTCCCAGAGG CTGGTGGAGC AGGAGGTTCA GGAGAAGCTG 
AGAGAGACCC AGGAGTATAA CCGAATTCAG AAGGAGCTGG AGAGAGAGAA AGCCAGCCTG 
ACTCTCTCAC TGATGGAAAA GGAACAGAGA CTCCTTGTTT TACAAGAAGC TGACTCTATT 
CGACAACAAG AGCTGAGTGC CCTGCGCCAG GACATGCAGG AGGCCCAGGG AGAACAGAAA 
GAGCTCAGTG CTCAGATGGA ATTACTAAGG CAAGAGGTGA AGGAAAAGGA GGCTGACTTT 
CTGGCCCAGG AAGCACAGCT GCTGGAGGAG CTGGAGGCGT CTCATATCAC GGAGCAGCAG 
CTGCGAGCCT CCTTGTGGGC CCAGGAAGCC AAGGCAGCCC AACTACAGCT GCGACTGCGC 
AGCACAGAGA GCCAGCTAGA AGCGCTGGCC GCAGAGCAGC AGCCCGGGAA CCAGGCCCAG 
GCCCAGGCCC AGCTGGCCAG CCTCTACTCT GCCCTGCAGC AGGCCCTGGG GTCTGTTTGT 
GAGAGCAGGC CTGAGCTGAG TGGTGGGGGA GACTCTGCTC CTTCCGTCTG GGGCCTTGAG 
CCAGACCAGA ATGGAGCTAG GAGCCTCTTT AAGAGAGGGC CCCTGCTGAC TGCTCTCTCC 
GCTGAGGCAG TAGCATCTGC CCTCCACAAG CTTCATCAAG ACCTGTGGAA GACTCAACAG 
ACCCGGGATG TTCTGAGGGA TCAGGTCCAG AAACTGGAAG AGCGTCTAAC TGATACTGAG 
GCTGAGAAGA GCCAGGTCCA CACAGAGTTG CAGGATCTGC AGAGACAGCT CTCCCAGAAT 
CAGGAAGAGA AATCTAAGTG GGAAGGAAAG CAGAACTCCC TAGAATCTGA GCTGATGGAA 
CTACATGAAA CTATGGCATC CTTACAGAGT CGCCTGCGGA GAGCAGAGCT ACAGCGAATG 
GAAGCCCAGG GTGAGCGAGA GTTACTTCAG GCAGCCAAGG AGAACCTGAC AGCCCAGGTG 
GAACACCTGC AAGCAGCTGT CGTAGAAGCC AGGGCTCAGG CAAGTGCTGC TGGCATCCTG 
GAAGAAGACC TGAGAACGGC TCGCTCAGCA CTGAAGCTGA AAAATGAGGA AGTAGAGAGT 
GAGCGTGAGA GAGCCCAGGC TCTGCAAGAG CAGGGCGAAC TGAAGGTGGC CCAAGGGAAG 
GCTCTGCAAG AGAATTTGGC CCTCCTGACC CAGACCCTAG CTGAAAGAGA AGAGGAGGTG 
GAGACTCTGC GGGGACAAAT CCAGGAACTG GAGAAGCAAC GGGAAATGCA GAAGGCTGCT 
TTGGAATTGC TGTCTCTGGA CCTGAAGAAG AGGAACCAAG AGGTAGATCT GCAGCAAGAA 
CAGATTCAGG AGCTAGAGAA GTGTAGGTCT GTTTTAGAGC ATCTGCCCAT GGCCGTCCAG 
GAGCGAGAGC AGAAGCTGAC TGTGCAGAGG GAGCAGATCA GAGAGCTCGA GAAGGATCGG 
GAGACTCAGA GGAACGTCTT GGAGCATCAG CTTCTAGAAC TTGAGAAGAA AGACCAAATG 
ATTGAGTCCC AGAGAGGACA GGTTCAGGAC CTGAAAAAGC AGTTGGTTAC TCTGGAATGC 
CTGGCCCTGG AACTGGAGGA AAACCATCAC AAGATGGAGT GCCAGCAAAA ACTGATCAAG 
GAGCTGGAGG GCCAGAGGGA AACCCAGAGA GTGGCTTTGA CCCACCTTAC GCTGGACCTA 
GAAGAAAGGA GCCAGGAGCT GCAGGCACAA AGCAGCCAGA TCCATGACCT GGAGAGCCAC 
AGCACCGTTC TGGCAAGAGA GCTGCAGGAG AGGGACCAGG AGGTGAAGTC TCAGCGAGAA 
CAGATCGAGG AGCTGCAGAG GCAGAAAGAG CATCTGACTC AGGATCTCGA GAGGAGAGAC 
CAGGAGCTGA TGCTGCAGAA GGAGAGGATT CAGGTTCTCG AGGATCAGAG GACCCGGCAG 
ACCAAGATCC TGGAGGAGGA CCTGGAACAG ATCAAGCTGT CCTTGAGAGA GCGAGGCCGG 
GAGCTGACCA CTCAGAGGCA GCTGATGCAG GAACGGGCAG AGGAAGGGAA GGGCCCAAGT 
AAAGCACAGC GCGGGAGCCT AGAGCACATG AAGCTGATCC TGCGTGATAA GGAGAAGGAG 
GTGGAATGTC AGCAGGAGCA TATCCATGAA CTCCAGGAGC TCAAAGACCA GCTGGAGCAG 
CAGCTCCAGG GCCTGCACAG GAAGGTAGGT GAGACCAGCC TCCTCCTGTC CCAGCGAGAG 
CAGGAAATAG TGGTCCTGCA GCAGCAACTG CAGGAAGCCA GGGAACAAGG GGAGCTGAAG 
GAGCAGTCAC TTCAGAGTCA ACTGGATGAG GCCCAGAGAG CCCTAGCCCA GAGGGACCAG 
GAACTGGAGG CTCTGCAGCA AGAACAGCAG CAGGCCCAGG GACAGGAGGA GAGGGTGAAG 
GAAAAGGCAG ACGCCCTCCA GGGAGCTCTG GAGCAAGCCC ATATGACACT GAAGGAGCGT 
CATGGAGAGC TTCAGGACCA CAAGGAACAG GCACGAAGGC TGGAGGAAGA GCTGGCAGTG 
GAGGGACGGC GGGTCCAGGC CCTGGAGGAG GTGCTGGGAG ACCTAAGGGC TGAGTCTCGG 
GAACAGGAGA AAGCTCTGTT GGCCCTCCAG CAGCAGTGTG CTGAGCAGGC ACAGGAGCAT 
GAGGTGGAGA CCAGGGCCCT GCAGGACAGC TGGCTGCAGG CCCAGGCAGT GCTCAAGGAA 
CGGGACCAGG AGCTGGAAGC TCTGCGGGCA GAAAGTCAGT CCTCCCGGCA TCAGGAGGAG 
GCTGCCCGGG CCCGGGCTGA GGCTCTGCAG GAGGCCCTTG GCAAGGCTCA TGCTGCCCTG 
CAGGGGAAAG AGCAGCATCT CCTCGAGCAG GCAGAATTGA GCCGCAGTCT GGAGGCCAGC 
ACTGCAACCC TGCAAGCCTC CCTGGATGCC TGCCAGGCAC ACAGTCGGCA GCTGGAGGAG 
GCTCTGAGGA TACAAGAAGG TGAGATCCAG GACCAGGATC TCCGATACCA GGAGGATGTG 
CAGCAGCTGC AGCAGGCACT TGCCCAGAGG GATGAAGAGC TGAGACATCA GCAGGAACGG 
GAGCAGCTGC TGGAGAAGTC TCTGGCCCAG AGGGTCCAAG AGAATATGAT CCAAGAGAAG 
CAGAATCTGG GGCAAGAGAG AGAAGAGGAG GAGATAAGGG GCCTTCATCA GAGTGTAAGG 
GAGCTACAGC TGACTCTAGC CCAAAAGGAA CAGGAGATTC TGGAGCTGAG GGAGACCCAG 
CAAAGGAACA ACCTGGAAGC CTTACCCCAC AGCCACAAAA CCTCCCCAAT GGAGGAACAA 
TCTCTAAAAC TTGATTCTTT AGAGCCCAGG CTGCAGCGGG AGCTGGAGCG GCTACAGGCA 
GCCCTGAGAC AGACAGAAGC CAGGGAGATT GAGTGGAGGG AGAAGGCCCA GGACTTGGCA 
CTCTCCCTAG CGCAGACCAA GGCCAGTGTC AGCAGTCTGC AGGAGGTAGC CATGTTCCTA 
CAAGCCTCTG TCCTGGAGCG GGACTCAGAA CAGCAAAGGC TGCAGGATGA ACTGGAGCTC 
ACCAGACGGG CTCTGGAGAA GGAGCGGCTA CACAGCCCAG GTGCAACCAG CACAGCAGAA 
CTGGGGTCCA GAGGGGAGCA GGGTGTGCAG CTGGGAGAGG TCTCAGGAGT GGAGGCTGAG 
CCTAGTCCTG ATGGAATGGA GAAGCAGTCA TGGAGACAAA GGCTTGAACA CCTGCAGCAA 
GCAGTGGCCC GGCTGGAGAT TGACAGGAGC AGGCTGCAGC GCCACAATGT CCAGCTGCGG 
AGTACCTTGG AGCAGGTGGA GCGAGAACGG AGGAAGCTGA AGAGGGAGGC CATGCGTGCG 
GCCCAGGCAG GGTCCCTAGA GATCAGCAAG GCCACGGCTT CTTCACCCAC ACAGCAGGAT 
GGGAGAGGAC AGAAGAACTC AGATGCCAAG TGTGTGGCTG AACTGCAGAA AGAGGTGGTC 
CTGCTGCAAG CTCAGCTGAC TTTGGAGCGG AAGCAGAAGC AGGACTACAT CACCCGCTCA 
GCACAGACCA GCCGTGAGCT AGCAGGCCTG CACCACAGCC TCTCACACTC ACTTCTTGCC 
GTGGCCCAGG CCCCTGAGGC CACTGTCCTG GAGGCAGAGA CCCGCAGGCT GGATGAGTCC 
CTGACTCAAA GTCTGACATC CCCAGGGCCA GTCCTGCTAC ACCCCAGCCC CAGCACTACC 
CAAGCCGCCT CCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_011528518.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526820.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

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CloneID	OHu64832
Clone ID Related Accession (Same CDS sequence)	XM_011528518.2 , XM_011528518.3
Accession Version	XM_011528518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCC CTTAGGGAAG ATGTGGAAAA ACTGACAGTG 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTTGTGACAT TCCGACGCCA CTTCCTGGAA ATGAAGTCAG CTACTGACAG AGATCTGATG 
GAGCTAAAAG CTGAGCATGT GAGGCTTTCA GGGTCTCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACAGTCTCG GGAACCCAAC GGATCTGGAA GAATGGATGG GCGGGAGCCG 
GCCCAGCTGC TGCTGCTACT AGCCAAGACC CAGGAGCTGG AGAAGGAAGC CCATGAAAGG 
AGCCAGGAGT TAATACAGCT GAAGAGTCAA GGGGATCTGG AGAAGGCTGA ACTTCAGGAC 
CGGGTGACCG AGCTCTCTGC TCTGTTGACC CAGTCTCAGA AGCAAAATGA AGATTATGAA 
AAGATGATAA AGGCTCTGAG AGAGACAGTG GAGATCCTGG AGACAAATCA CACAGAATTA 
ATGGAACATG AAGCATCTCT TAGTAGGAAT GCGCAAGAGG AGAAGTTGTC TTTACAGCAG 
GTGATCAAGG ATATAACCCA GGTCATGGTG GAAGAAGGGG ACAATATAGC CCAAGGCTCT 
GGTCATGAGA ACTCTTTGGA ATTGGACTCT AGTATCTTCT CCCAGTTTGA TTACCAGGAT 
GCAGACAAGG CTCTTACTCT GGTGCGTTCA GTGCTGACTC GGAGACGCCA GGCTGTGCAG 
GACCTAAGGC AGCAGCTTGC AGGCTGTCAA GAGGCTGTGA ACTTGTTGCA ACAGCAGCAT 
GATCAGTGGG AGGAAGAGGG CAAAGCCTTG AGACAGCGGC TGCAGAAGCT CACTGGGGAG 
CGGGACACTC TGGCAGGGCA GACTGTGGAC CTCCAGGGAG AGGTGGACTC TCTCAGCAAG 
GAGCGAGAGC TGCTGCAGAA GGCCAGGGAA GAGCTGCGGC AGCAGCTGGA GGTGCTAGAG 
CAGGAGGCAT GGCGCCTGCG AAGGGTAAAT GTGGAGCTTC AGCTGCAGGG GGACTCTGCC 
CAGGGCCAGA AGGAGGAACA GCAGGAGGAG CTGCACCTGG CTGTCCGGGA GAGGGAGCGT 
CTTCAGGAGA TGCTGATGGG CCTGGAAGCC AAACAGTCAG AATCACTCAG TGAACTGATC 
ACTCTTCGGG AAGCCCTGGA GTCAAGTCAC CTGGAAGGGG AGTTACTGAG GCAAGAGCAA 
ACGGAAGTGA CCGCAGCGCT GGCTAGGGCA GAGCAGTCAA TTGCAGAGCT GTCGAGTTCT 
GAAAACACCC TGAAGACAGA AGTAGCTGAT CTTCGGGCTG CAGCTGTCAA GCTCAGTGCC 
TTAAATGAGG CTTTGGCGTT AGATAAAGTT GGGCTGAACC AGCAGCTTCT CCAGTTAGAG 
GAGGAGAACC AGTCTGTGTG CAGCAGAATG GAGGCCGCAG AGCAGGCGAG AAATGCTTTG 
CAGGTCGACC TGGCGGAGGC AGAGAAGAGG AGGGAAGCCC TGTGGGAAAA GAACACTCAC 
CTGGAGGCTC AGCTGCAGAA AGCTGAGGAG GCTGGGGCTG AGCTGCAGGC AGATCTCAGG 
GACATCCAAG AAGAGAAGGA AGAAATTCAA AAGAAACTAA GTGAGTCACG TCACCAGCAG 
GAGGCAGCCA CGACTCAGCT GGAGCAGCTA CATCAGGAGG CAAAGCGACA GGAAGAAGTG 
CTTGCCAGGG CAGTCCAGGA GAAGGAGGCC CTAGTACGAG AGAAAGCGGC TCTAGAGGTG 
CGGCTGCAGG CCGTGGAGCG TGACCGGCAG GACCTCGCTG AACAACTACA GGGGCTCAGC 
TCAGCCAAGG AGCTACTGGA GAGCAGTCTG TTTGAAGCCC AACAACAAAA TTCTGTGATA 
GAGGTCACCA AGGGGCAGCT GGAGGTCCAG ATTCAAACTG TCACTCAAGC CAAGGAAGTA 
ATCCAAGGGG AAGTGAGGTG CCTGAAGCTG GAACTGGACA CTGAACGGAG TCAGGCAGAG 
CAGGAGCGGG ATGCTGCAGC CAGACAGCTG GCCCAGGCTG AGCAAGAAGG GAAGACTGCC 
TTGGAGCAGC AGAAGGCAGC CCATGAGAAA GAGGTGAACC AGCTCCGGGA GAAATGGGAG 
AAGGAGCGCT CCTGGCACCA GCAGGAGCTG GCAAAGGCTC TGGAGAGCTT AGAAAGGGAA 
AAAATGGAGC TGGAAATGAG GCTAAAGGAG CAGCAGACAG AAATGGAGGC CATCCAGGCC 
CAGAGGGAAG AAGAACGGAC CCAGGCAGAG AGTGCCCTAT GCCAGATGCA GCTGGAAACA 
GAGAAGGAGA GAGTATCCCT CCTGGAGACA CTGCTGCAGA CGCAGAAGGA GCTAGCAGAT 
GCCAGCCAAC AACTGGAACG ACTGAGGCAG GACATGAAAG TCCAGAAATT AAAGGAGCAG 
GAGACCACTG GGATACTACA GACCCAGCTC CAGGAGGCTC AACGGGAGCT GAAGGAGGCA 
GCCCGGCAGC ACAGAGATGA CCTTGCTGCC CTCCAAGAAG AGAGCAGCTC CCTGCTGCAG 
GATAAGATGG ACCTGCAGAA GCAGGTGGAG GACTTGAAGT CTCAGCTGGT GGCCCAGGAT 
GACTCCCAGA GGCTGGTGGA GCAGGAGGTT CAGGAGAAGC TGAGAGAGAC CCAGGAGTAT 
AACCGAATTC AGAAGGAGCT GGAGAGAGAG AAAGCCAGCC TGACTCTCTC ACTGATGGAA 
AAGGAACAGA GACTCCTTGT TTTACAAGAA GCTGACTCTA TTCGACAACA AGAGCTGAGT 
GCCCTGCGCC AGGACATGCA GGAGGCCCAG GGAGAACAGA AAGAGCTCAG TGCTCAGATG 
GAATTACTAA GGCAAGAGGT GAAGGAAAAG GAGGCTGACT TTCTGGCCCA GGAAGCACAG 
CTGCTGGAGG AGCTGGAGGC GTCTCATATC ACGGAGCAGC AGCTGCGAGC CTCCTTGTGG 
GCCCAGGAAG CCAAGGCAGC CCAACTACAG CTGCGACTGC GCAGCACAGA GAGCCAGCTA 
GAAGCGCTGG CCGCAGAGCA GCAGCCCGGG AACCAGGCCC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTACT CTGCCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGTGGGG GAGACTCTGC TCCTTCCGTC TGGGGCCTTG AGCCAGACCA GAATGGAGCT 
AGGAGCCTCT TTAAGAGAGG GCCCCTGCTG ACTGCTCTCT CCGCTGAGGC AGTAGCATCT 
GCCCTCCACA AGCTTCATCA AGACCTGTGG AAGACTCAAC AGACCCGGGA TGTTCTGAGG 
GATCAGGTCC AGAAACTGGA AGAGCGTCTA ACTGATACTG AGGCTGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGATCT GCAGAGACAG CTCTCCCAGA ATCAGGAAGA GAAATCTAAG 
TGGGAAGGAA AGCAGAACTC CCTAGAATCT GAGCTGATGG AACTACATGA AACTATGGCA 
TCCTTACAGA GTCGCCTGCG GAGAGCAGAG CTACAGCGAA TGGAAGCCCA GGGTGAGCGA 
GAGTTACTTC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT GCAAGCAGCT 
GTCGTAGAAG CCAGGGCTCA GGCAAGTGCT GCTGGCATCC TGGAAGAAGA CCTGAGAACG 
GCTCGCTCAG CACTGAAGCT GAAAAATGAG GAAGTAGAGA GTGAGCGTGA GAGAGCCCAG 
GCTCTGCAAG AGCAGGGCGA ACTGAAGGTG GCCCAAGGGA AGGCTCTGCA AGAGAATTTG 
GCCCTCCTGA CCCAGACCCT AGCTGAAAGA GAAGAGGAGG TGGAGACTCT GCGGGGACAA 
ATCCAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG CTTTGGAATT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCA AGAGGTAGAT CTGCAGCAAG AACAGATTCA GGAGCTAGAG 
AAGTGTAGGT CTGTTTTAGA GCATCTGCCC ATGGCCGTCC AGGAGCGAGA GCAGAAGCTG 
ACTGTGCAGA GGGAGCAGAT CAGAGAGCTC GAGAAGGATC GGGAGACTCA GAGGAACGTC 
TTGGAGCATC AGCTTCTAGA ACTTGAGAAG AAAGACCAAA TGATTGAGTC CCAGAGAGGA 
CAGGTTCAGG ACCTGAAAAA GCAGTTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTGGAG 
GAAAACCATC ACAAGATGGA GTGCCAGCAA AAACTGATCA AGGAGCTGGA GGGCCAGAGG 
GAAACCCAGA GAGTGGCTTT GACCCACCTT ACGCTGGACC TAGAAGAAAG GAGCCAGGAG 
CTGCAGGCAC AAAGCAGCCA GATCCATGAC CTGGAGAGCC ACAGCACCGT TCTGGCAAGA 
GAGCTGCAGG AGAGGGACCA GGAGGTGAAG TCTCAGCGAG AACAGATCGA GGAGCTGCAG 
AGGCAGAAAG AGCATCTGAC TCAGGATCTC GAGAGGAGAG ACCAGGAGCT GATGCTGCAG 
AAGGAGAGGA TTCAGGTTCT CGAGGATCAG AGGACCCGGC AGACCAAGAT CCTGGAGGAG 
GACCTGGAAC AGATCAAGCT GTCCTTGAGA GAGCGAGGCC GGGAGCTGAC CACTCAGAGG 
CAGCTGATGC AGGAACGGGC AGAGGAAGGG AAGGGCCCAA GTAAAGCACA GCGCGGGAGC 
CTAGAGCACA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG TCAGCAGGAG 
CATATCCATG AACTCCAGGA GCTCAAAGAC CAGCTGGAGC AGCAGCTCCA GGGCCTGCAC 
AGGAAGGTAG GTGAGACCAG CCTCCTCCTG TCCCAGCGAG AGCAGGAAAT AGTGGTCCTG 
CAGCAGCAAC TGCAGGAAGC CAGGGAACAA GGGGAGCTGA AGGAGCAGTC ACTTCAGAGT 
CAACTGGATG AGGCCCAGAG AGCCCTAGCC CAGAGGGACC AGGAACTGGA GGCTCTGCAG 
CAAGAACAGC AGCAGGCCCA GGGACAGGAG GAGAGGGTGA AGGAAAAGGC AGACGCCCTC 
CAGGGAGCTC TGGAGCAAGC CCATATGACA CTGAAGGAGC GTCATGGAGA GCTTCAGGAC 
CACAAGGAAC AGGCACGAAG GCTGGAGGAA GAGCTGGCAG TGGAGGGACG GCGGGTCCAG 
GCCCTGGAGG AGGTGCTGGG AGACCTAAGG GCTGAGTCTC GGGAACAGGA GAAAGCTCTG 
TTGGCCCTCC AGCAGCAGTG TGCTGAGCAG GCACAGGAGC ATGAGGTGGA GACCAGGGCC 
CTGCAGGACA GCTGGCTGCA GGCCCAGGCA GTGCTCAAGG AACGGGACCA GGAGCTGGAA 
GCTCTGCGGG CAGAAAGTCA GTCCTCCCGG CATCAGGAGG AGGCTGCCCG GGCCCGGGCT 
GAGGCTCTGC AGGAGGCCCT TGGCAAGGCT CATGCTGCCC TGCAGGGGAA AGAGCAGCAT 
CTCCTCGAGC AGGCAGAATT GAGCCGCAGT CTGGAGGCCA GCACTGCAAC CCTGCAAGCC 
TCCCTGGATG CCTGCCAGGC ACACAGTCGG CAGCTGGAGG AGGCTCTGAG GATACAAGAA 
GGTGAGATCC AGGACCAGGA TCTCCGATAC CAGGAGGATG TGCAGCAGCT GCAGCAGGCA 
CTTGCCCAGA GGGATGAAGA GCTGAGACAT CAGCAGGAAC GGGAGCAGCT GCTGGAGAAG 
TCTCTGGCCC AGAGGGTCCA AGAGAATATG ATCCAAGAGA AGCAGAATCT GGGGCAAGAG 
AGAGAAGAGG AGGAGATAAG GGGCCTTCAT CAGAGTGTAA GGGAGCTACA GCTGACTCTA 
GCCCAAAAGG AACAGGAGAT TCTGGAGCTG AGGGAGACCC AGCAAAGGAA CAACCTGGAA 
GCCTTACCCC ACAGCCACAA AACCTCCCCA ATGGAGGAAC AATCTCTAAA ACTTGATTCT 
TTAGAGCCCA GGCTGCAGCG GGAGCTGGAG CGGCTACAGG CAGCCCTGAG ACAGACAGAA 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTCTCCCT AGCGCAGACC 
AAGGCCAGTG TCAGCAGTCT GCAGGAGGTA GCCATGTTCC TACAAGCCTC TGTCCTGGAG 
CGGGACTCAG AACAGCAAAG GCTGCAGGAT GAACTGGAGC TCACCAGACG GGCTCTGGAG 
AAGGAGCGGC TACACAGCCC AGGTGCAACC AGCACAGCAG AACTGGGGTC CAGAGGGGAG 
CAGGGTGTGC AGCTGGGAGA GGTCTCAGGA GTGGAGGCTG AGCCTAGTCC TGATGGAATG 
GAGAAGCAGT CATGGAGACA AAGGCTTGAA CACCTGCAGC AAGCAGTGGC CCGGCTGGAG 
ATTGACAGGA GCAGGCTGCA GCGCCACAAT GTCCAGCTGC GGAGTACCTT GGAGCAGGTG 
GAGCGAGAAC GGAGGAAGCT GAAGAGGGAG GCCATGCGTG CGGCCCAGGC AGGGTCCCTA 
GAGATCAGCA AGGCCACGGC TTCTTCACCC ACACAGCAGG ATGGGAGAGG ACAGAAGAAC 
TCAGATGCCA AGTGTGTGGC TGAACTGCAG AAAGAGGTGG TCCTGCTGCA AGCTCAGCTG 
ACTTTGGAGC GGAAGCAGAA GCAGGACTAC ATCACCCGCT CAGCACAGAC CAGCCGTGAG 
CTAGCAGGCC TGCACCACAG CCTCTCACAC TCACTTCTTG CCGTGGCCCA GGCCCCTGAG 
GCCACTGTCC TGGAGGCAGA GACCCGCAGG CTGGATGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGTCCTGCT ACACCCCAGC CCCAGCACTA CCCAAGCCGC CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526820.1
CDS	774..7733
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_011528518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528518.2

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCC CTTAGGGAAG ATGTGGAAAA ACTGACAGTG 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTTGTGACAT TCCGACGCCA CTTCCTGGAA ATGAAGTCAG CTACTGACAG AGATCTGATG 
GAGCTAAAAG CTGAGCATGT GAGGCTTTCA GGGTCTCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACAGTCTCG GGAACCCAAC GGATCTGGAA GAATGGATGG GCGGGAGCCG 
GCCCAGCTGC TGCTGCTACT AGCCAAGACC CAGGAGCTGG AGAAGGAAGC CCATGAAAGG 
AGCCAGGAGT TAATACAGCT GAAGAGTCAA GGGGATCTGG AGAAGGCTGA ACTTCAGGAC 
CGGGTGACCG AGCTCTCTGC TCTGTTGACC CAGTCTCAGA AGCAAAATGA AGATTATGAA 
AAGATGATAA AGGCTCTGAG AGAGACAGTG GAGATCCTGG AGACAAATCA CACAGAATTA 
ATGGAACATG AAGCATCTCT TAGTAGGAAT GCGCAAGAGG AGAAGTTGTC TTTACAGCAG 
GTGATCAAGG ATATAACCCA GGTCATGGTG GAAGAAGGGG ACAATATAGC CCAAGGCTCT 
GGTCATGAGA ACTCTTTGGA ATTGGACTCT AGTATCTTCT CCCAGTTTGA TTACCAGGAT 
GCAGACAAGG CTCTTACTCT GGTGCGTTCA GTGCTGACTC GGAGACGCCA GGCTGTGCAG 
GACCTAAGGC AGCAGCTTGC AGGCTGTCAA GAGGCTGTGA ACTTGTTGCA ACAGCAGCAT 
GATCAGTGGG AGGAAGAGGG CAAAGCCTTG AGACAGCGGC TGCAGAAGCT CACTGGGGAG 
CGGGACACTC TGGCAGGGCA GACTGTGGAC CTCCAGGGAG AGGTGGACTC TCTCAGCAAG 
GAGCGAGAGC TGCTGCAGAA GGCCAGGGAA GAGCTGCGGC AGCAGCTGGA GGTGCTAGAG 
CAGGAGGCAT GGCGCCTGCG AAGGGTAAAT GTGGAGCTTC AGCTGCAGGG GGACTCTGCC 
CAGGGCCAGA AGGAGGAACA GCAGGAGGAG CTGCACCTGG CTGTCCGGGA GAGGGAGCGT 
CTTCAGGAGA TGCTGATGGG CCTGGAAGCC AAACAGTCAG AATCACTCAG TGAACTGATC 
ACTCTTCGGG AAGCCCTGGA GTCAAGTCAC CTGGAAGGGG AGTTACTGAG GCAAGAGCAA 
ACGGAAGTGA CCGCAGCGCT GGCTAGGGCA GAGCAGTCAA TTGCAGAGCT GTCGAGTTCT 
GAAAACACCC TGAAGACAGA AGTAGCTGAT CTTCGGGCTG CAGCTGTCAA GCTCAGTGCC 
TTAAATGAGG CTTTGGCGTT AGATAAAGTT GGGCTGAACC AGCAGCTTCT CCAGTTAGAG 
GAGGAGAACC AGTCTGTGTG CAGCAGAATG GAGGCCGCAG AGCAGGCGAG AAATGCTTTG 
CAGGTCGACC TGGCGGAGGC AGAGAAGAGG AGGGAAGCCC TGTGGGAAAA GAACACTCAC 
CTGGAGGCTC AGCTGCAGAA AGCTGAGGAG GCTGGGGCTG AGCTGCAGGC AGATCTCAGG 
GACATCCAAG AAGAGAAGGA AGAAATTCAA AAGAAACTAA GTGAGTCACG TCACCAGCAG 
GAGGCAGCCA CGACTCAGCT GGAGCAGCTA CATCAGGAGG CAAAGCGACA GGAAGAAGTG 
CTTGCCAGGG CAGTCCAGGA GAAGGAGGCC CTAGTACGAG AGAAAGCGGC TCTAGAGGTG 
CGGCTGCAGG CCGTGGAGCG TGACCGGCAG GACCTCGCTG AACAACTACA GGGGCTCAGC 
TCAGCCAAGG AGCTACTGGA GAGCAGTCTG TTTGAAGCCC AACAACAAAA TTCTGTGATA 
GAGGTCACCA AGGGGCAGCT GGAGGTCCAG ATTCAAACTG TCACTCAAGC CAAGGAAGTA 
ATCCAAGGGG AAGTGAGGTG CCTGAAGCTG GAACTGGACA CTGAACGGAG TCAGGCAGAG 
CAGGAGCGGG ATGCTGCAGC CAGACAGCTG GCCCAGGCTG AGCAAGAAGG GAAGACTGCC 
TTGGAGCAGC AGAAGGCAGC CCATGAGAAA GAGGTGAACC AGCTCCGGGA GAAATGGGAG 
AAGGAGCGCT CCTGGCACCA GCAGGAGCTG GCAAAGGCTC TGGAGAGCTT AGAAAGGGAA 
AAAATGGAGC TGGAAATGAG GCTAAAGGAG CAGCAGACAG AAATGGAGGC CATCCAGGCC 
CAGAGGGAAG AAGAACGGAC CCAGGCAGAG AGTGCCCTAT GCCAGATGCA GCTGGAAACA 
GAGAAGGAGA GAGTATCCCT CCTGGAGACA CTGCTGCAGA CGCAGAAGGA GCTAGCAGAT 
GCCAGCCAAC AACTGGAACG ACTGAGGCAG GACATGAAAG TCCAGAAATT AAAGGAGCAG 
GAGACCACTG GGATACTACA GACCCAGCTC CAGGAGGCTC AACGGGAGCT GAAGGAGGCA 
GCCCGGCAGC ACAGAGATGA CCTTGCTGCC CTCCAAGAAG AGAGCAGCTC CCTGCTGCAG 
GATAAGATGG ACCTGCAGAA GCAGGTGGAG GACTTGAAGT CTCAGCTGGT GGCCCAGGAT 
GACTCCCAGA GGCTGGTGGA GCAGGAGGTT CAGGAGAAGC TGAGAGAGAC CCAGGAGTAT 
AACCGAATTC AGAAGGAGCT GGAGAGAGAG AAAGCCAGCC TGACTCTCTC ACTGATGGAA 
AAGGAACAGA GACTCCTTGT TTTACAAGAA GCTGACTCTA TTCGACAACA AGAGCTGAGT 
GCCCTGCGCC AGGACATGCA GGAGGCCCAG GGAGAACAGA AAGAGCTCAG TGCTCAGATG 
GAATTACTAA GGCAAGAGGT GAAGGAAAAG GAGGCTGACT TTCTGGCCCA GGAAGCACAG 
CTGCTGGAGG AGCTGGAGGC GTCTCATATC ACGGAGCAGC AGCTGCGAGC CTCCTTGTGG 
GCCCAGGAAG CCAAGGCAGC CCAACTACAG CTGCGACTGC GCAGCACAGA GAGCCAGCTA 
GAAGCGCTGG CCGCAGAGCA GCAGCCCGGG AACCAGGCCC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTACT CTGCCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGTGGGG GAGACTCTGC TCCTTCCGTC TGGGGCCTTG AGCCAGACCA GAATGGAGCT 
AGGAGCCTCT TTAAGAGAGG GCCCCTGCTG ACTGCTCTCT CCGCTGAGGC AGTAGCATCT 
GCCCTCCACA AGCTTCATCA AGACCTGTGG AAGACTCAAC AGACCCGGGA TGTTCTGAGG 
GATCAGGTCC AGAAACTGGA AGAGCGTCTA ACTGATACTG AGGCTGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGATCT GCAGAGACAG CTCTCCCAGA ATCAGGAAGA GAAATCTAAG 
TGGGAAGGAA AGCAGAACTC CCTAGAATCT GAGCTGATGG AACTACATGA AACTATGGCA 
TCCTTACAGA GTCGCCTGCG GAGAGCAGAG CTACAGCGAA TGGAAGCCCA GGGTGAGCGA 
GAGTTACTTC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT GCAAGCAGCT 
GTCGTAGAAG CCAGGGCTCA GGCAAGTGCT GCTGGCATCC TGGAAGAAGA CCTGAGAACG 
GCTCGCTCAG CACTGAAGCT GAAAAATGAG GAAGTAGAGA GTGAGCGTGA GAGAGCCCAG 
GCTCTGCAAG AGCAGGGCGA ACTGAAGGTG GCCCAAGGGA AGGCTCTGCA AGAGAATTTG 
GCCCTCCTGA CCCAGACCCT AGCTGAAAGA GAAGAGGAGG TGGAGACTCT GCGGGGACAA 
ATCCAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG CTTTGGAATT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCA AGAGGTAGAT CTGCAGCAAG AACAGATTCA GGAGCTAGAG 
AAGTGTAGGT CTGTTTTAGA GCATCTGCCC ATGGCCGTCC AGGAGCGAGA GCAGAAGCTG 
ACTGTGCAGA GGGAGCAGAT CAGAGAGCTC GAGAAGGATC GGGAGACTCA GAGGAACGTC 
TTGGAGCATC AGCTTCTAGA ACTTGAGAAG AAAGACCAAA TGATTGAGTC CCAGAGAGGA 
CAGGTTCAGG ACCTGAAAAA GCAGTTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTGGAG 
GAAAACCATC ACAAGATGGA GTGCCAGCAA AAACTGATCA AGGAGCTGGA GGGCCAGAGG 
GAAACCCAGA GAGTGGCTTT GACCCACCTT ACGCTGGACC TAGAAGAAAG GAGCCAGGAG 
CTGCAGGCAC AAAGCAGCCA GATCCATGAC CTGGAGAGCC ACAGCACCGT TCTGGCAAGA 
GAGCTGCAGG AGAGGGACCA GGAGGTGAAG TCTCAGCGAG AACAGATCGA GGAGCTGCAG 
AGGCAGAAAG AGCATCTGAC TCAGGATCTC GAGAGGAGAG ACCAGGAGCT GATGCTGCAG 
AAGGAGAGGA TTCAGGTTCT CGAGGATCAG AGGACCCGGC AGACCAAGAT CCTGGAGGAG 
GACCTGGAAC AGATCAAGCT GTCCTTGAGA GAGCGAGGCC GGGAGCTGAC CACTCAGAGG 
CAGCTGATGC AGGAACGGGC AGAGGAAGGG AAGGGCCCAA GTAAAGCACA GCGCGGGAGC 
CTAGAGCACA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG TCAGCAGGAG 
CATATCCATG AACTCCAGGA GCTCAAAGAC CAGCTGGAGC AGCAGCTCCA GGGCCTGCAC 
AGGAAGGTAG GTGAGACCAG CCTCCTCCTG TCCCAGCGAG AGCAGGAAAT AGTGGTCCTG 
CAGCAGCAAC TGCAGGAAGC CAGGGAACAA GGGGAGCTGA AGGAGCAGTC ACTTCAGAGT 
CAACTGGATG AGGCCCAGAG AGCCCTAGCC CAGAGGGACC AGGAACTGGA GGCTCTGCAG 
CAAGAACAGC AGCAGGCCCA GGGACAGGAG GAGAGGGTGA AGGAAAAGGC AGACGCCCTC 
CAGGGAGCTC TGGAGCAAGC CCATATGACA CTGAAGGAGC GTCATGGAGA GCTTCAGGAC 
CACAAGGAAC AGGCACGAAG GCTGGAGGAA GAGCTGGCAG TGGAGGGACG GCGGGTCCAG 
GCCCTGGAGG AGGTGCTGGG AGACCTAAGG GCTGAGTCTC GGGAACAGGA GAAAGCTCTG 
TTGGCCCTCC AGCAGCAGTG TGCTGAGCAG GCACAGGAGC ATGAGGTGGA GACCAGGGCC 
CTGCAGGACA GCTGGCTGCA GGCCCAGGCA GTGCTCAAGG AACGGGACCA GGAGCTGGAA 
GCTCTGCGGG CAGAAAGTCA GTCCTCCCGG CATCAGGAGG AGGCTGCCCG GGCCCGGGCT 
GAGGCTCTGC AGGAGGCCCT TGGCAAGGCT CATGCTGCCC TGCAGGGGAA AGAGCAGCAT 
CTCCTCGAGC AGGCAGAATT GAGCCGCAGT CTGGAGGCCA GCACTGCAAC CCTGCAAGCC 
TCCCTGGATG CCTGCCAGGC ACACAGTCGG CAGCTGGAGG AGGCTCTGAG GATACAAGAA 
GGTGAGATCC AGGACCAGGA TCTCCGATAC CAGGAGGATG TGCAGCAGCT GCAGCAGGCA 
CTTGCCCAGA GGGATGAAGA GCTGAGACAT CAGCAGGAAC GGGAGCAGCT GCTGGAGAAG 
TCTCTGGCCC AGAGGGTCCA AGAGAATATG ATCCAAGAGA AGCAGAATCT GGGGCAAGAG 
AGAGAAGAGG AGGAGATAAG GGGCCTTCAT CAGAGTGTAA GGGAGCTACA GCTGACTCTA 
GCCCAAAAGG AACAGGAGAT TCTGGAGCTG AGGGAGACCC AGCAAAGGAA CAACCTGGAA 
GCCTTACCCC ACAGCCACAA AACCTCCCCA ATGGAGGAAC AATCTCTAAA ACTTGATTCT 
TTAGAGCCCA GGCTGCAGCG GGAGCTGGAG CGGCTACAGG CAGCCCTGAG ACAGACAGAA 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTCTCCCT AGCGCAGACC 
AAGGCCAGTG TCAGCAGTCT GCAGGAGGTA GCCATGTTCC TACAAGCCTC TGTCCTGGAG 
CGGGACTCAG AACAGCAAAG GCTGCAGGAT GAACTGGAGC TCACCAGACG GGCTCTGGAG 
AAGGAGCGGC TACACAGCCC AGGTGCAACC AGCACAGCAG AACTGGGGTC CAGAGGGGAG 
CAGGGTGTGC AGCTGGGAGA GGTCTCAGGA GTGGAGGCTG AGCCTAGTCC TGATGGAATG 
GAGAAGCAGT CATGGAGACA AAGGCTTGAA CACCTGCAGC AAGCAGTGGC CCGGCTGGAG 
ATTGACAGGA GCAGGCTGCA GCGCCACAAT GTCCAGCTGC GGAGTACCTT GGAGCAGGTG 
GAGCGAGAAC GGAGGAAGCT GAAGAGGGAG GCCATGCGTG CGGCCCAGGC AGGGTCCCTA 
GAGATCAGCA AGGCCACGGC TTCTTCACCC ACACAGCAGG ATGGGAGAGG ACAGAAGAAC 
TCAGATGCCA AGTGTGTGGC TGAACTGCAG AAAGAGGTGG TCCTGCTGCA AGCTCAGCTG 
ACTTTGGAGC GGAAGCAGAA GCAGGACTAC ATCACCCGCT CAGCACAGAC CAGCCGTGAG 
CTAGCAGGCC TGCACCACAG CCTCTCACAC TCACTTCTTG CCGTGGCCCA GGCCCCTGAG 
GCCACTGTCC TGGAGGCAGA GACCCGCAGG CTGGATGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGTCCTGCT ACACCCCAGC CCCAGCACTA CCCAAGCCGC CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_011528518.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526820.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

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CloneID	OHu64832
Clone ID Related Accession (Same CDS sequence)	XM_011528518.2 , XM_011528518.3
Accession Version	XM_011528518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCC CTTAGGGAAG ATGTGGAAAA ACTGACAGTG 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTTGTGACAT TCCGACGCCA CTTCCTGGAA ATGAAGTCAG CTACTGACAG AGATCTGATG 
GAGCTAAAAG CTGAGCATGT GAGGCTTTCA GGGTCTCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACAGTCTCG GGAACCCAAC GGATCTGGAA GAATGGATGG GCGGGAGCCG 
GCCCAGCTGC TGCTGCTACT AGCCAAGACC CAGGAGCTGG AGAAGGAAGC CCATGAAAGG 
AGCCAGGAGT TAATACAGCT GAAGAGTCAA GGGGATCTGG AGAAGGCTGA ACTTCAGGAC 
CGGGTGACCG AGCTCTCTGC TCTGTTGACC CAGTCTCAGA AGCAAAATGA AGATTATGAA 
AAGATGATAA AGGCTCTGAG AGAGACAGTG GAGATCCTGG AGACAAATCA CACAGAATTA 
ATGGAACATG AAGCATCTCT TAGTAGGAAT GCGCAAGAGG AGAAGTTGTC TTTACAGCAG 
GTGATCAAGG ATATAACCCA GGTCATGGTG GAAGAAGGGG ACAATATAGC CCAAGGCTCT 
GGTCATGAGA ACTCTTTGGA ATTGGACTCT AGTATCTTCT CCCAGTTTGA TTACCAGGAT 
GCAGACAAGG CTCTTACTCT GGTGCGTTCA GTGCTGACTC GGAGACGCCA GGCTGTGCAG 
GACCTAAGGC AGCAGCTTGC AGGCTGTCAA GAGGCTGTGA ACTTGTTGCA ACAGCAGCAT 
GATCAGTGGG AGGAAGAGGG CAAAGCCTTG AGACAGCGGC TGCAGAAGCT CACTGGGGAG 
CGGGACACTC TGGCAGGGCA GACTGTGGAC CTCCAGGGAG AGGTGGACTC TCTCAGCAAG 
GAGCGAGAGC TGCTGCAGAA GGCCAGGGAA GAGCTGCGGC AGCAGCTGGA GGTGCTAGAG 
CAGGAGGCAT GGCGCCTGCG AAGGGTAAAT GTGGAGCTTC AGCTGCAGGG GGACTCTGCC 
CAGGGCCAGA AGGAGGAACA GCAGGAGGAG CTGCACCTGG CTGTCCGGGA GAGGGAGCGT 
CTTCAGGAGA TGCTGATGGG CCTGGAAGCC AAACAGTCAG AATCACTCAG TGAACTGATC 
ACTCTTCGGG AAGCCCTGGA GTCAAGTCAC CTGGAAGGGG AGTTACTGAG GCAAGAGCAA 
ACGGAAGTGA CCGCAGCGCT GGCTAGGGCA GAGCAGTCAA TTGCAGAGCT GTCGAGTTCT 
GAAAACACCC TGAAGACAGA AGTAGCTGAT CTTCGGGCTG CAGCTGTCAA GCTCAGTGCC 
TTAAATGAGG CTTTGGCGTT AGATAAAGTT GGGCTGAACC AGCAGCTTCT CCAGTTAGAG 
GAGGAGAACC AGTCTGTGTG CAGCAGAATG GAGGCCGCAG AGCAGGCGAG AAATGCTTTG 
CAGGTCGACC TGGCGGAGGC AGAGAAGAGG AGGGAAGCCC TGTGGGAAAA GAACACTCAC 
CTGGAGGCTC AGCTGCAGAA AGCTGAGGAG GCTGGGGCTG AGCTGCAGGC AGATCTCAGG 
GACATCCAAG AAGAGAAGGA AGAAATTCAA AAGAAACTAA GTGAGTCACG TCACCAGCAG 
GAGGCAGCCA CGACTCAGCT GGAGCAGCTA CATCAGGAGG CAAAGCGACA GGAAGAAGTG 
CTTGCCAGGG CAGTCCAGGA GAAGGAGGCC CTAGTACGAG AGAAAGCGGC TCTAGAGGTG 
CGGCTGCAGG CCGTGGAGCG TGACCGGCAG GACCTCGCTG AACAACTACA GGGGCTCAGC 
TCAGCCAAGG AGCTACTGGA GAGCAGTCTG TTTGAAGCCC AACAACAAAA TTCTGTGATA 
GAGGTCACCA AGGGGCAGCT GGAGGTCCAG ATTCAAACTG TCACTCAAGC CAAGGAAGTA 
ATCCAAGGGG AAGTGAGGTG CCTGAAGCTG GAACTGGACA CTGAACGGAG TCAGGCAGAG 
CAGGAGCGGG ATGCTGCAGC CAGACAGCTG GCCCAGGCTG AGCAAGAAGG GAAGACTGCC 
TTGGAGCAGC AGAAGGCAGC CCATGAGAAA GAGGTGAACC AGCTCCGGGA GAAATGGGAG 
AAGGAGCGCT CCTGGCACCA GCAGGAGCTG GCAAAGGCTC TGGAGAGCTT AGAAAGGGAA 
AAAATGGAGC TGGAAATGAG GCTAAAGGAG CAGCAGACAG AAATGGAGGC CATCCAGGCC 
CAGAGGGAAG AAGAACGGAC CCAGGCAGAG AGTGCCCTAT GCCAGATGCA GCTGGAAACA 
GAGAAGGAGA GAGTATCCCT CCTGGAGACA CTGCTGCAGA CGCAGAAGGA GCTAGCAGAT 
GCCAGCCAAC AACTGGAACG ACTGAGGCAG GACATGAAAG TCCAGAAATT AAAGGAGCAG 
GAGACCACTG GGATACTACA GACCCAGCTC CAGGAGGCTC AACGGGAGCT GAAGGAGGCA 
GCCCGGCAGC ACAGAGATGA CCTTGCTGCC CTCCAAGAAG AGAGCAGCTC CCTGCTGCAG 
GATAAGATGG ACCTGCAGAA GCAGGTGGAG GACTTGAAGT CTCAGCTGGT GGCCCAGGAT 
GACTCCCAGA GGCTGGTGGA GCAGGAGGTT CAGGAGAAGC TGAGAGAGAC CCAGGAGTAT 
AACCGAATTC AGAAGGAGCT GGAGAGAGAG AAAGCCAGCC TGACTCTCTC ACTGATGGAA 
AAGGAACAGA GACTCCTTGT TTTACAAGAA GCTGACTCTA TTCGACAACA AGAGCTGAGT 
GCCCTGCGCC AGGACATGCA GGAGGCCCAG GGAGAACAGA AAGAGCTCAG TGCTCAGATG 
GAATTACTAA GGCAAGAGGT GAAGGAAAAG GAGGCTGACT TTCTGGCCCA GGAAGCACAG 
CTGCTGGAGG AGCTGGAGGC GTCTCATATC ACGGAGCAGC AGCTGCGAGC CTCCTTGTGG 
GCCCAGGAAG CCAAGGCAGC CCAACTACAG CTGCGACTGC GCAGCACAGA GAGCCAGCTA 
GAAGCGCTGG CCGCAGAGCA GCAGCCCGGG AACCAGGCCC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTACT CTGCCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGTGGGG GAGACTCTGC TCCTTCCGTC TGGGGCCTTG AGCCAGACCA GAATGGAGCT 
AGGAGCCTCT TTAAGAGAGG GCCCCTGCTG ACTGCTCTCT CCGCTGAGGC AGTAGCATCT 
GCCCTCCACA AGCTTCATCA AGACCTGTGG AAGACTCAAC AGACCCGGGA TGTTCTGAGG 
GATCAGGTCC AGAAACTGGA AGAGCGTCTA ACTGATACTG AGGCTGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGATCT GCAGAGACAG CTCTCCCAGA ATCAGGAAGA GAAATCTAAG 
TGGGAAGGAA AGCAGAACTC CCTAGAATCT GAGCTGATGG AACTACATGA AACTATGGCA 
TCCTTACAGA GTCGCCTGCG GAGAGCAGAG CTACAGCGAA TGGAAGCCCA GGGTGAGCGA 
GAGTTACTTC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT GCAAGCAGCT 
GTCGTAGAAG CCAGGGCTCA GGCAAGTGCT GCTGGCATCC TGGAAGAAGA CCTGAGAACG 
GCTCGCTCAG CACTGAAGCT GAAAAATGAG GAAGTAGAGA GTGAGCGTGA GAGAGCCCAG 
GCTCTGCAAG AGCAGGGCGA ACTGAAGGTG GCCCAAGGGA AGGCTCTGCA AGAGAATTTG 
GCCCTCCTGA CCCAGACCCT AGCTGAAAGA GAAGAGGAGG TGGAGACTCT GCGGGGACAA 
ATCCAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG CTTTGGAATT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCA AGAGGTAGAT CTGCAGCAAG AACAGATTCA GGAGCTAGAG 
AAGTGTAGGT CTGTTTTAGA GCATCTGCCC ATGGCCGTCC AGGAGCGAGA GCAGAAGCTG 
ACTGTGCAGA GGGAGCAGAT CAGAGAGCTC GAGAAGGATC GGGAGACTCA GAGGAACGTC 
TTGGAGCATC AGCTTCTAGA ACTTGAGAAG AAAGACCAAA TGATTGAGTC CCAGAGAGGA 
CAGGTTCAGG ACCTGAAAAA GCAGTTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTGGAG 
GAAAACCATC ACAAGATGGA GTGCCAGCAA AAACTGATCA AGGAGCTGGA GGGCCAGAGG 
GAAACCCAGA GAGTGGCTTT GACCCACCTT ACGCTGGACC TAGAAGAAAG GAGCCAGGAG 
CTGCAGGCAC AAAGCAGCCA GATCCATGAC CTGGAGAGCC ACAGCACCGT TCTGGCAAGA 
GAGCTGCAGG AGAGGGACCA GGAGGTGAAG TCTCAGCGAG AACAGATCGA GGAGCTGCAG 
AGGCAGAAAG AGCATCTGAC TCAGGATCTC GAGAGGAGAG ACCAGGAGCT GATGCTGCAG 
AAGGAGAGGA TTCAGGTTCT CGAGGATCAG AGGACCCGGC AGACCAAGAT CCTGGAGGAG 
GACCTGGAAC AGATCAAGCT GTCCTTGAGA GAGCGAGGCC GGGAGCTGAC CACTCAGAGG 
CAGCTGATGC AGGAACGGGC AGAGGAAGGG AAGGGCCCAA GTAAAGCACA GCGCGGGAGC 
CTAGAGCACA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG TCAGCAGGAG 
CATATCCATG AACTCCAGGA GCTCAAAGAC CAGCTGGAGC AGCAGCTCCA GGGCCTGCAC 
AGGAAGGTAG GTGAGACCAG CCTCCTCCTG TCCCAGCGAG AGCAGGAAAT AGTGGTCCTG 
CAGCAGCAAC TGCAGGAAGC CAGGGAACAA GGGGAGCTGA AGGAGCAGTC ACTTCAGAGT 
CAACTGGATG AGGCCCAGAG AGCCCTAGCC CAGAGGGACC AGGAACTGGA GGCTCTGCAG 
CAAGAACAGC AGCAGGCCCA GGGACAGGAG GAGAGGGTGA AGGAAAAGGC AGACGCCCTC 
CAGGGAGCTC TGGAGCAAGC CCATATGACA CTGAAGGAGC GTCATGGAGA GCTTCAGGAC 
CACAAGGAAC AGGCACGAAG GCTGGAGGAA GAGCTGGCAG TGGAGGGACG GCGGGTCCAG 
GCCCTGGAGG AGGTGCTGGG AGACCTAAGG GCTGAGTCTC GGGAACAGGA GAAAGCTCTG 
TTGGCCCTCC AGCAGCAGTG TGCTGAGCAG GCACAGGAGC ATGAGGTGGA GACCAGGGCC 
CTGCAGGACA GCTGGCTGCA GGCCCAGGCA GTGCTCAAGG AACGGGACCA GGAGCTGGAA 
GCTCTGCGGG CAGAAAGTCA GTCCTCCCGG CATCAGGAGG AGGCTGCCCG GGCCCGGGCT 
GAGGCTCTGC AGGAGGCCCT TGGCAAGGCT CATGCTGCCC TGCAGGGGAA AGAGCAGCAT 
CTCCTCGAGC AGGCAGAATT GAGCCGCAGT CTGGAGGCCA GCACTGCAAC CCTGCAAGCC 
TCCCTGGATG CCTGCCAGGC ACACAGTCGG CAGCTGGAGG AGGCTCTGAG GATACAAGAA 
GGTGAGATCC AGGACCAGGA TCTCCGATAC CAGGAGGATG TGCAGCAGCT GCAGCAGGCA 
CTTGCCCAGA GGGATGAAGA GCTGAGACAT CAGCAGGAAC GGGAGCAGCT GCTGGAGAAG 
TCTCTGGCCC AGAGGGTCCA AGAGAATATG ATCCAAGAGA AGCAGAATCT GGGGCAAGAG 
AGAGAAGAGG AGGAGATAAG GGGCCTTCAT CAGAGTGTAA GGGAGCTACA GCTGACTCTA 
GCCCAAAAGG AACAGGAGAT TCTGGAGCTG AGGGAGACCC AGCAAAGGAA CAACCTGGAA 
GCCTTACCCC ACAGCCACAA AACCTCCCCA ATGGAGGAAC AATCTCTAAA ACTTGATTCT 
TTAGAGCCCA GGCTGCAGCG GGAGCTGGAG CGGCTACAGG CAGCCCTGAG ACAGACAGAA 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTCTCCCT AGCGCAGACC 
AAGGCCAGTG TCAGCAGTCT GCAGGAGGTA GCCATGTTCC TACAAGCCTC TGTCCTGGAG 
CGGGACTCAG AACAGCAAAG GCTGCAGGAT GAACTGGAGC TCACCAGACG GGCTCTGGAG 
AAGGAGCGGC TACACAGCCC AGGTGCAACC AGCACAGCAG AACTGGGGTC CAGAGGGGAG 
CAGGGTGTGC AGCTGGGAGA GGTCTCAGGA GTGGAGGCTG AGCCTAGTCC TGATGGAATG 
GAGAAGCAGT CATGGAGACA AAGGCTTGAA CACCTGCAGC AAGCAGTGGC CCGGCTGGAG 
ATTGACAGGA GCAGGCTGCA GCGCCACAAT GTCCAGCTGC GGAGTACCTT GGAGCAGGTG 
GAGCGAGAAC GGAGGAAGCT GAAGAGGGAG GCCATGCGTG CGGCCCAGGC AGGGTCCCTA 
GAGATCAGCA AGGCCACGGC TTCTTCACCC ACACAGCAGG ATGGGAGAGG ACAGAAGAAC 
TCAGATGCCA AGTGTGTGGC TGAACTGCAG AAAGAGGTGG TCCTGCTGCA AGCTCAGCTG 
ACTTTGGAGC GGAAGCAGAA GCAGGACTAC ATCACCCGCT CAGCACAGAC CAGCCGTGAG 
CTAGCAGGCC TGCACCACAG CCTCTCACAC TCACTTCTTG CCGTGGCCCA GGCCCCTGAG 
GCCACTGTCC TGGAGGCAGA GACCCGCAGG CTGGATGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGTCCTGCT ACACCCCAGC CCCAGCACTA CCCAAGCCGC CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526820.1
CDS	775..7734
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_011528518.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528518.3

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCC CTTAGGGAAG ATGTGGAAAA ACTGACAGTG 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTTGTGACAT TCCGACGCCA CTTCCTGGAA ATGAAGTCAG CTACTGACAG AGATCTGATG 
GAGCTAAAAG CTGAGCATGT GAGGCTTTCA GGGTCTCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACAGTCTCG GGAACCCAAC GGATCTGGAA GAATGGATGG GCGGGAGCCG 
GCCCAGCTGC TGCTGCTACT AGCCAAGACC CAGGAGCTGG AGAAGGAAGC CCATGAAAGG 
AGCCAGGAGT TAATACAGCT GAAGAGTCAA GGGGATCTGG AGAAGGCTGA ACTTCAGGAC 
CGGGTGACCG AGCTCTCTGC TCTGTTGACC CAGTCTCAGA AGCAAAATGA AGATTATGAA 
AAGATGATAA AGGCTCTGAG AGAGACAGTG GAGATCCTGG AGACAAATCA CACAGAATTA 
ATGGAACATG AAGCATCTCT TAGTAGGAAT GCGCAAGAGG AGAAGTTGTC TTTACAGCAG 
GTGATCAAGG ATATAACCCA GGTCATGGTG GAAGAAGGGG ACAATATAGC CCAAGGCTCT 
GGTCATGAGA ACTCTTTGGA ATTGGACTCT AGTATCTTCT CCCAGTTTGA TTACCAGGAT 
GCAGACAAGG CTCTTACTCT GGTGCGTTCA GTGCTGACTC GGAGACGCCA GGCTGTGCAG 
GACCTAAGGC AGCAGCTTGC AGGCTGTCAA GAGGCTGTGA ACTTGTTGCA ACAGCAGCAT 
GATCAGTGGG AGGAAGAGGG CAAAGCCTTG AGACAGCGGC TGCAGAAGCT CACTGGGGAG 
CGGGACACTC TGGCAGGGCA GACTGTGGAC CTCCAGGGAG AGGTGGACTC TCTCAGCAAG 
GAGCGAGAGC TGCTGCAGAA GGCCAGGGAA GAGCTGCGGC AGCAGCTGGA GGTGCTAGAG 
CAGGAGGCAT GGCGCCTGCG AAGGGTAAAT GTGGAGCTTC AGCTGCAGGG GGACTCTGCC 
CAGGGCCAGA AGGAGGAACA GCAGGAGGAG CTGCACCTGG CTGTCCGGGA GAGGGAGCGT 
CTTCAGGAGA TGCTGATGGG CCTGGAAGCC AAACAGTCAG AATCACTCAG TGAACTGATC 
ACTCTTCGGG AAGCCCTGGA GTCAAGTCAC CTGGAAGGGG AGTTACTGAG GCAAGAGCAA 
ACGGAAGTGA CCGCAGCGCT GGCTAGGGCA GAGCAGTCAA TTGCAGAGCT GTCGAGTTCT 
GAAAACACCC TGAAGACAGA AGTAGCTGAT CTTCGGGCTG CAGCTGTCAA GCTCAGTGCC 
TTAAATGAGG CTTTGGCGTT AGATAAAGTT GGGCTGAACC AGCAGCTTCT CCAGTTAGAG 
GAGGAGAACC AGTCTGTGTG CAGCAGAATG GAGGCCGCAG AGCAGGCGAG AAATGCTTTG 
CAGGTCGACC TGGCGGAGGC AGAGAAGAGG AGGGAAGCCC TGTGGGAAAA GAACACTCAC 
CTGGAGGCTC AGCTGCAGAA AGCTGAGGAG GCTGGGGCTG AGCTGCAGGC AGATCTCAGG 
GACATCCAAG AAGAGAAGGA AGAAATTCAA AAGAAACTAA GTGAGTCACG TCACCAGCAG 
GAGGCAGCCA CGACTCAGCT GGAGCAGCTA CATCAGGAGG CAAAGCGACA GGAAGAAGTG 
CTTGCCAGGG CAGTCCAGGA GAAGGAGGCC CTAGTACGAG AGAAAGCGGC TCTAGAGGTG 
CGGCTGCAGG CCGTGGAGCG TGACCGGCAG GACCTCGCTG AACAACTACA GGGGCTCAGC 
TCAGCCAAGG AGCTACTGGA GAGCAGTCTG TTTGAAGCCC AACAACAAAA TTCTGTGATA 
GAGGTCACCA AGGGGCAGCT GGAGGTCCAG ATTCAAACTG TCACTCAAGC CAAGGAAGTA 
ATCCAAGGGG AAGTGAGGTG CCTGAAGCTG GAACTGGACA CTGAACGGAG TCAGGCAGAG 
CAGGAGCGGG ATGCTGCAGC CAGACAGCTG GCCCAGGCTG AGCAAGAAGG GAAGACTGCC 
TTGGAGCAGC AGAAGGCAGC CCATGAGAAA GAGGTGAACC AGCTCCGGGA GAAATGGGAG 
AAGGAGCGCT CCTGGCACCA GCAGGAGCTG GCAAAGGCTC TGGAGAGCTT AGAAAGGGAA 
AAAATGGAGC TGGAAATGAG GCTAAAGGAG CAGCAGACAG AAATGGAGGC CATCCAGGCC 
CAGAGGGAAG AAGAACGGAC CCAGGCAGAG AGTGCCCTAT GCCAGATGCA GCTGGAAACA 
GAGAAGGAGA GAGTATCCCT CCTGGAGACA CTGCTGCAGA CGCAGAAGGA GCTAGCAGAT 
GCCAGCCAAC AACTGGAACG ACTGAGGCAG GACATGAAAG TCCAGAAATT AAAGGAGCAG 
GAGACCACTG GGATACTACA GACCCAGCTC CAGGAGGCTC AACGGGAGCT GAAGGAGGCA 
GCCCGGCAGC ACAGAGATGA CCTTGCTGCC CTCCAAGAAG AGAGCAGCTC CCTGCTGCAG 
GATAAGATGG ACCTGCAGAA GCAGGTGGAG GACTTGAAGT CTCAGCTGGT GGCCCAGGAT 
GACTCCCAGA GGCTGGTGGA GCAGGAGGTT CAGGAGAAGC TGAGAGAGAC CCAGGAGTAT 
AACCGAATTC AGAAGGAGCT GGAGAGAGAG AAAGCCAGCC TGACTCTCTC ACTGATGGAA 
AAGGAACAGA GACTCCTTGT TTTACAAGAA GCTGACTCTA TTCGACAACA AGAGCTGAGT 
GCCCTGCGCC AGGACATGCA GGAGGCCCAG GGAGAACAGA AAGAGCTCAG TGCTCAGATG 
GAATTACTAA GGCAAGAGGT GAAGGAAAAG GAGGCTGACT TTCTGGCCCA GGAAGCACAG 
CTGCTGGAGG AGCTGGAGGC GTCTCATATC ACGGAGCAGC AGCTGCGAGC CTCCTTGTGG 
GCCCAGGAAG CCAAGGCAGC CCAACTACAG CTGCGACTGC GCAGCACAGA GAGCCAGCTA 
GAAGCGCTGG CCGCAGAGCA GCAGCCCGGG AACCAGGCCC AGGCCCAGGC CCAGCTGGCC 
AGCCTCTACT CTGCCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGTGGGG GAGACTCTGC TCCTTCCGTC TGGGGCCTTG AGCCAGACCA GAATGGAGCT 
AGGAGCCTCT TTAAGAGAGG GCCCCTGCTG ACTGCTCTCT CCGCTGAGGC AGTAGCATCT 
GCCCTCCACA AGCTTCATCA AGACCTGTGG AAGACTCAAC AGACCCGGGA TGTTCTGAGG 
GATCAGGTCC AGAAACTGGA AGAGCGTCTA ACTGATACTG AGGCTGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGATCT GCAGAGACAG CTCTCCCAGA ATCAGGAAGA GAAATCTAAG 
TGGGAAGGAA AGCAGAACTC CCTAGAATCT GAGCTGATGG AACTACATGA AACTATGGCA 
TCCTTACAGA GTCGCCTGCG GAGAGCAGAG CTACAGCGAA TGGAAGCCCA GGGTGAGCGA 
GAGTTACTTC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT GCAAGCAGCT 
GTCGTAGAAG CCAGGGCTCA GGCAAGTGCT GCTGGCATCC TGGAAGAAGA CCTGAGAACG 
GCTCGCTCAG CACTGAAGCT GAAAAATGAG GAAGTAGAGA GTGAGCGTGA GAGAGCCCAG 
GCTCTGCAAG AGCAGGGCGA ACTGAAGGTG GCCCAAGGGA AGGCTCTGCA AGAGAATTTG 
GCCCTCCTGA CCCAGACCCT AGCTGAAAGA GAAGAGGAGG TGGAGACTCT GCGGGGACAA 
ATCCAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG CTTTGGAATT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCA AGAGGTAGAT CTGCAGCAAG AACAGATTCA GGAGCTAGAG 
AAGTGTAGGT CTGTTTTAGA GCATCTGCCC ATGGCCGTCC AGGAGCGAGA GCAGAAGCTG 
ACTGTGCAGA GGGAGCAGAT CAGAGAGCTC GAGAAGGATC GGGAGACTCA GAGGAACGTC 
TTGGAGCATC AGCTTCTAGA ACTTGAGAAG AAAGACCAAA TGATTGAGTC CCAGAGAGGA 
CAGGTTCAGG ACCTGAAAAA GCAGTTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTGGAG 
GAAAACCATC ACAAGATGGA GTGCCAGCAA AAACTGATCA AGGAGCTGGA GGGCCAGAGG 
GAAACCCAGA GAGTGGCTTT GACCCACCTT ACGCTGGACC TAGAAGAAAG GAGCCAGGAG 
CTGCAGGCAC AAAGCAGCCA GATCCATGAC CTGGAGAGCC ACAGCACCGT TCTGGCAAGA 
GAGCTGCAGG AGAGGGACCA GGAGGTGAAG TCTCAGCGAG AACAGATCGA GGAGCTGCAG 
AGGCAGAAAG AGCATCTGAC TCAGGATCTC GAGAGGAGAG ACCAGGAGCT GATGCTGCAG 
AAGGAGAGGA TTCAGGTTCT CGAGGATCAG AGGACCCGGC AGACCAAGAT CCTGGAGGAG 
GACCTGGAAC AGATCAAGCT GTCCTTGAGA GAGCGAGGCC GGGAGCTGAC CACTCAGAGG 
CAGCTGATGC AGGAACGGGC AGAGGAAGGG AAGGGCCCAA GTAAAGCACA GCGCGGGAGC 
CTAGAGCACA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG TCAGCAGGAG 
CATATCCATG AACTCCAGGA GCTCAAAGAC CAGCTGGAGC AGCAGCTCCA GGGCCTGCAC 
AGGAAGGTAG GTGAGACCAG CCTCCTCCTG TCCCAGCGAG AGCAGGAAAT AGTGGTCCTG 
CAGCAGCAAC TGCAGGAAGC CAGGGAACAA GGGGAGCTGA AGGAGCAGTC ACTTCAGAGT 
CAACTGGATG AGGCCCAGAG AGCCCTAGCC CAGAGGGACC AGGAACTGGA GGCTCTGCAG 
CAAGAACAGC AGCAGGCCCA GGGACAGGAG GAGAGGGTGA AGGAAAAGGC AGACGCCCTC 
CAGGGAGCTC TGGAGCAAGC CCATATGACA CTGAAGGAGC GTCATGGAGA GCTTCAGGAC 
CACAAGGAAC AGGCACGAAG GCTGGAGGAA GAGCTGGCAG TGGAGGGACG GCGGGTCCAG 
GCCCTGGAGG AGGTGCTGGG AGACCTAAGG GCTGAGTCTC GGGAACAGGA GAAAGCTCTG 
TTGGCCCTCC AGCAGCAGTG TGCTGAGCAG GCACAGGAGC ATGAGGTGGA GACCAGGGCC 
CTGCAGGACA GCTGGCTGCA GGCCCAGGCA GTGCTCAAGG AACGGGACCA GGAGCTGGAA 
GCTCTGCGGG CAGAAAGTCA GTCCTCCCGG CATCAGGAGG AGGCTGCCCG GGCCCGGGCT 
GAGGCTCTGC AGGAGGCCCT TGGCAAGGCT CATGCTGCCC TGCAGGGGAA AGAGCAGCAT 
CTCCTCGAGC AGGCAGAATT GAGCCGCAGT CTGGAGGCCA GCACTGCAAC CCTGCAAGCC 
TCCCTGGATG CCTGCCAGGC ACACAGTCGG CAGCTGGAGG AGGCTCTGAG GATACAAGAA 
GGTGAGATCC AGGACCAGGA TCTCCGATAC CAGGAGGATG TGCAGCAGCT GCAGCAGGCA 
CTTGCCCAGA GGGATGAAGA GCTGAGACAT CAGCAGGAAC GGGAGCAGCT GCTGGAGAAG 
TCTCTGGCCC AGAGGGTCCA AGAGAATATG ATCCAAGAGA AGCAGAATCT GGGGCAAGAG 
AGAGAAGAGG AGGAGATAAG GGGCCTTCAT CAGAGTGTAA GGGAGCTACA GCTGACTCTA 
GCCCAAAAGG AACAGGAGAT TCTGGAGCTG AGGGAGACCC AGCAAAGGAA CAACCTGGAA 
GCCTTACCCC ACAGCCACAA AACCTCCCCA ATGGAGGAAC AATCTCTAAA ACTTGATTCT 
TTAGAGCCCA GGCTGCAGCG GGAGCTGGAG CGGCTACAGG CAGCCCTGAG ACAGACAGAA 
GCCAGGGAGA TTGAGTGGAG GGAGAAGGCC CAGGACTTGG CACTCTCCCT AGCGCAGACC 
AAGGCCAGTG TCAGCAGTCT GCAGGAGGTA GCCATGTTCC TACAAGCCTC TGTCCTGGAG 
CGGGACTCAG AACAGCAAAG GCTGCAGGAT GAACTGGAGC TCACCAGACG GGCTCTGGAG 
AAGGAGCGGC TACACAGCCC AGGTGCAACC AGCACAGCAG AACTGGGGTC CAGAGGGGAG 
CAGGGTGTGC AGCTGGGAGA GGTCTCAGGA GTGGAGGCTG AGCCTAGTCC TGATGGAATG 
GAGAAGCAGT CATGGAGACA AAGGCTTGAA CACCTGCAGC AAGCAGTGGC CCGGCTGGAG 
ATTGACAGGA GCAGGCTGCA GCGCCACAAT GTCCAGCTGC GGAGTACCTT GGAGCAGGTG 
GAGCGAGAAC GGAGGAAGCT GAAGAGGGAG GCCATGCGTG CGGCCCAGGC AGGGTCCCTA 
GAGATCAGCA AGGCCACGGC TTCTTCACCC ACACAGCAGG ATGGGAGAGG ACAGAAGAAC 
TCAGATGCCA AGTGTGTGGC TGAACTGCAG AAAGAGGTGG TCCTGCTGCA AGCTCAGCTG 
ACTTTGGAGC GGAAGCAGAA GCAGGACTAC ATCACCCGCT CAGCACAGAC CAGCCGTGAG 
CTAGCAGGCC TGCACCACAG CCTCTCACAC TCACTTCTTG CCGTGGCCCA GGCCCCTGAG 
GCCACTGTCC TGGAGGCAGA GACCCGCAGG CTGGATGAGT CCCTGACTCA AAGTCTGACA 
TCCCCAGGGC CAGTCCTGCT ACACCCCAGC CCCAGCACTA CCCAAGCCGC CTCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_011528519.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526821.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X12, mRNA.

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CloneID	OHu64833
Clone ID Related Accession (Same CDS sequence)	XM_011528519.2
Accession Version	XM_011528519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAA AAGCTGAGCA TGTGAGGCTT TCAGGGTCTC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACAGTC TCGGGAACCC AACGGATCTG GAAGAATGGA TGGGCGGGAG 
CCGGCCCAGC TGCTGCTGCT ACTAGCCAAG ACCCAGGAGC TGGAGAAGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC TGAACTTCAG 
GACCGGGTGA CCGAGCTCTC TGCTCTGTTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGA TAAAGGCTCT GAGAGAGACA GTGGAGATCC TGGAGACAAA TCACACAGAA 
TTAATGGAAC ATGAAGCATC TCTTAGTAGG AATGCGCAAG AGGAGAAGTT GTCTTTACAG 
CAGGTGATCA AGGATATAAC CCAGGTCATG GTGGAAGAAG GGGACAATAT AGCCCAAGGC 
TCTGGTCATG AGAACTCTTT GGAATTGGAC TCTAGTATCT TCTCCCAGTT TGATTACCAG 
GATGCAGACA AGGCTCTTAC TCTGGTGCGT TCAGTGCTGA CTCGGAGACG CCAGGCTGTG 
CAGGACCTAA GGCAGCAGCT TGCAGGCTGT CAAGAGGCTG TGAACTTGTT GCAACAGCAG 
CATGATCAGT GGGAGGAAGA GGGCAAAGCC TTGAGACAGC GGCTGCAGAA GCTCACTGGG 
GAGCGGGACA CTCTGGCAGG GCAGACTGTG GACCTCCAGG GAGAGGTGGA CTCTCTCAGC 
AAGGAGCGAG AGCTGCTGCA GAAGGCCAGG GAAGAGCTGC GGCAGCAGCT GGAGGTGCTA 
GAGCAGGAGG CATGGCGCCT GCGAAGGGTA AATGTGGAGC TTCAGCTGCA GGGGGACTCT 
GCCCAGGGCC AGAAGGAGGA ACAGCAGGAG GAGCTGCACC TGGCTGTCCG GGAGAGGGAG 
CGTCTTCAGG AGATGCTGAT GGGCCTGGAA GCCAAACAGT CAGAATCACT CAGTGAACTG 
ATCACTCTTC GGGAAGCCCT GGAGTCAAGT CACCTGGAAG GGGAGTTACT GAGGCAAGAG 
CAAACGGAAG TGACCGCAGC GCTGGCTAGG GCAGAGCAGT CAATTGCAGA GCTGTCGAGT 
TCTGAAAACA CCCTGAAGAC AGAAGTAGCT GATCTTCGGG CTGCAGCTGT CAAGCTCAGT 
GCCTTAAATG AGGCTTTGGC GTTAGATAAA GTTGGGCTGA ACCAGCAGCT TCTCCAGTTA 
GAGGAGGAGA ACCAGTCTGT GTGCAGCAGA ATGGAGGCCG CAGAGCAGGC GAGAAATGCT 
TTGCAGGTCG ACCTGGCGGA GGCAGAGAAG AGGAGGGAAG CCCTGTGGGA AAAGAACACT 
CACCTGGAGG CTCAGCTGCA GAAAGCTGAG GAGGCTGGGG CTGAGCTGCA GGCAGATCTC 
AGGGACATCC AAGAAGAGAA GGAAGAAATT CAAAAGAAAC TAAGTGAGTC ACGTCACCAG 
CAGGAGGCAG CCACGACTCA GCTGGAGCAG CTACATCAGG AGGCAAAGCG ACAGGAAGAA 
GTGCTTGCCA GGGCAGTCCA GGAGAAGGAG GCCCTAGTAC GAGAGAAAGC GGCTCTAGAG 
GTGCGGCTGC AGGCCGTGGA GCGTGACCGG CAGGACCTCG CTGAACAACT ACAGGGGCTC 
AGCTCAGCCA AGGAGCTACT GGAGAGCAGT CTGTTTGAAG CCCAACAACA AAATTCTGTG 
ATAGAGGTCA CCAAGGGGCA GCTGGAGGTC CAGATTCAAA CTGTCACTCA AGCCAAGGAA 
GTAATCCAAG GGGAAGTGAG GTGCCTGAAG CTGGAACTGG ACACTGAACG GAGTCAGGCA 
GAGCAGGAGC GGGATGCTGC AGCCAGACAG CTGGCCCAGG CTGAGCAAGA AGGGAAGACT 
GCCTTGGAGC AGCAGAAGGC AGCCCATGAG AAAGAGGTGA ACCAGCTCCG GGAGAAATGG 
GAGAAGGAGC GCTCCTGGCA CCAGCAGGAG CTGGCAAAGG CTCTGGAGAG CTTAGAAAGG 
GAAAAAATGG AGCTGGAAAT GAGGCTAAAG GAGCAGCAGA CAGAAATGGA GGCCATCCAG 
GCCCAGAGGG AAGAAGAACG GACCCAGGCA GAGAGTGCCC TATGCCAGAT GCAGCTGGAA 
ACAGAGAAGG AGAGAGTATC CCTCCTGGAG ACACTGCTGC AGACGCAGAA GGAGCTAGCA 
GATGCCAGCC AACAACTGGA ACGACTGAGG CAGGACATGA AAGTCCAGAA ATTAAAGGAG 
CAGGAGACCA CTGGGATACT ACAGACCCAG CTCCAGGAGG CTCAACGGGA GCTGAAGGAG 
GCAGCCCGGC AGCACAGAGA TGACCTTGCT GCCCTCCAAG AAGAGAGCAG CTCCCTGCTG 
CAGGATAAGA TGGACCTGCA GAAGCAGGTG GAGGACTTGA AGTCTCAGCT GGTGGCCCAG 
GATGACTCCC AGAGGCTGGT GGAGCAGGAG GTTCAGGAGA AGCTGAGAGA GACCCAGGAG 
TATAACCGAA TTCAGAAGGA GCTGGAGAGA GAGAAAGCCA GCCTGACTCT CTCACTGATG 
GAAAAGGAAC AGAGACTCCT TGTTTTACAA GAAGCTGACT CTATTCGACA ACAAGAGCTG 
AGTGCCCTGC GCCAGGACAT GCAGGAGGCC CAGGGAGAAC AGAAAGAGCT CAGTGCTCAG 
ATGGAATTAC TAAGGCAAGA GGTGAAGGAA AAGGAGGCTG ACTTTCTGGC CCAGGAAGCA 
CAGCTGCTGG AGGAGCTGGA GGCGTCTCAT ATCACGGAGC AGCAGCTGCG AGCCTCCTTG 
TGGGCCCAGG AAGCCAAGGC AGCCCAACTA CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG 
CTAGAAGCGC TGGCCGCAGA GCAGCAGCCC GGGAACCAGG CCCAGGCCCA GGCCCAGCTG 
GCCAGCCTCT ACTCTGCCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGTG GGGGAGACTC TGCTCCTTCC GTCTGGGGCC TTGAGCCAGA CCAGAATGGA 
GCTAGGAGCC TCTTTAAGAG AGGGCCCCTG CTGACTGCTC TCTCCGCTGA GGCAGTAGCA 
TCTGCCCTCC ACAAGCTTCA TCAAGACCTG TGGAAGACTC AACAGACCCG GGATGTTCTG 
AGGGATCAGG TCCAGAAACT GGAAGAGCGT CTAACTGATA CTGAGGCTGA GAAGAGCCAG 
GTCCACACAG AGTTGCAGGA TCTGCAGAGA CAGCTCTCCC AGAATCAGGA AGAGAAATCT 
AAGTGGGAAG GAAAGCAGAA CTCCCTAGAA TCTGAGCTGA TGGAACTACA TGAAACTATG 
GCATCCTTAC AGAGTCGCCT GCGGAGAGCA GAGCTACAGC GAATGGAAGC CCAGGGTGAG 
CGAGAGTTAC TTCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTGCAAGCA 
GCTGTCGTAG AAGCCAGGGC TCAGGCAAGT GCTGCTGGCA TCCTGGAAGA AGACCTGAGA 
ACGGCTCGCT CAGCACTGAA GCTGAAAAAT GAGGAAGTAG AGAGTGAGCG TGAGAGAGCC 
CAGGCTCTGC AAGAGCAGGG CGAACTGAAG GTGGCCCAAG GGAAGGCTCT GCAAGAGAAT 
TTGGCCCTCC TGACCCAGAC CCTAGCTGAA AGAGAAGAGG AGGTGGAGAC TCTGCGGGGA 
CAAATCCAGG AACTGGAGAA GCAACGGGAA ATGCAGAAGG CTGCTTTGGA ATTGCTGTCT 
CTGGACCTGA AGAAGAGGAA CCAAGAGGTA GATCTGCAGC AAGAACAGAT TCAGGAGCTA 
GAGAAGTGTA GGTCTGTTTT AGAGCATCTG CCCATGGCCG TCCAGGAGCG AGAGCAGAAG 
CTGACTGTGC AGAGGGAGCA GATCAGAGAG CTCGAGAAGG ATCGGGAGAC TCAGAGGAAC 
GTCTTGGAGC ATCAGCTTCT AGAACTTGAG AAGAAAGACC AAATGATTGA GTCCCAGAGA 
GGACAGGTTC AGGACCTGAA AAAGCAGTTG GTTACTCTGG AATGCCTGGC CCTGGAACTG 
GAGGAAAACC ATCACAAGAT GGAGTGCCAG CAAAAACTGA TCAAGGAGCT GGAGGGCCAG 
AGGGAAACCC AGAGAGTGGC TTTGACCCAC CTTACGCTGG ACCTAGAAGA AAGGAGCCAG 
GAGCTGCAGG CACAAAGCAG CCAGATCCAT GACCTGGAGA GCCACAGCAC CGTTCTGGCA 
AGAGAGCTGC AGGAGAGGGA CCAGGAGGTG AAGTCTCAGC GAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AAGAGCATCT GACTCAGGAT CTCGAGAGGA GAGACCAGGA GCTGATGCTG 
CAGAAGGAGA GGATTCAGGT TCTCGAGGAT CAGAGGACCC GGCAGACCAA GATCCTGGAG 
GAGGACCTGG AACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GACCACTCAG 
AGGCAGCTGA TGCAGGAACG GGCAGAGGAA GGGAAGGGCC CAAGTAAAGC ACAGCGCGGG 
AGCCTAGAGC ACATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGTCAGCAG 
GAGCATATCC ATGAACTCCA GGAGCTCAAA GACCAGCTGG AGCAGCAGCT CCAGGGCCTG 
CACAGGAAGG TAGGTGAGAC CAGCCTCCTC CTGTCCCAGC GAGAGCAGGA AATAGTGGTC 
CTGCAGCAGC AACTGCAGGA AGCCAGGGAA CAAGGGGAGC TGAAGGAGCA GTCACTTCAG 
AGTCAACTGG ATGAGGCCCA GAGAGCCCTA GCCCAGAGGG ACCAGGAACT GGAGGCTCTG 
CAGCAAGAAC AGCAGCAGGC CCAGGGACAG GAGGAGAGGG TGAAGGAAAA GGCAGACGCC 
CTCCAGGGAG CTCTGGAGCA AGCCCATATG ACACTGAAGG AGCGTCATGG AGAGCTTCAG 
GACCACAAGG AACAGGCACG AAGGCTGGAG GAAGAGCTGG CAGTGGAGGG ACGGCGGGTC 
CAGGCCCTGG AGGAGGTGCT GGGAGACCTA AGGGCTGAGT CTCGGGAACA GGAGAAAGCT 
CTGTTGGCCC TCCAGCAGCA GTGTGCTGAG CAGGCACAGG AGCATGAGGT GGAGACCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCCCAG GCAGTGCTCA AGGAACGGGA CCAGGAGCTG 
GAAGCTCTGC GGGCAGAAAG TCAGTCCTCC CGGCATCAGG AGGAGGCTGC CCGGGCCCGG 
GCTGAGGCTC TGCAGGAGGC CCTTGGCAAG GCTCATGCTG CCCTGCAGGG GAAAGAGCAG 
CATCTCCTCG AGCAGGCAGA ATTGAGCCGC AGTCTGGAGG CCAGCACTGC AACCCTGCAA 
GCCTCCCTGG ATGCCTGCCA GGCACACAGT CGGCAGCTGG AGGAGGCTCT GAGGATACAA 
GAAGGTGAGA TCCAGGACCA GGATCTCCGA TACCAGGAGG ATGTGCAGCA GCTGCAGCAG 
GCACTTGCCC AGAGGGATGA AGAGCTGAGA CATCAGCAGG AACGGGAGCA GCTGCTGGAG 
AAGTCTCTGG CCCAGAGGGT CCAAGAGAAT ATGATCCAAG AGAAGCAGAA TCTGGGGCAA 
GAGAGAGAAG AGGAGGAGAT AAGGGGCCTT CATCAGAGTG TAAGGGAGCT ACAGCTGACT 
CTAGCCCAAA AGGAACAGGA GATTCTGGAG CTGAGGGAGA CCCAGCAAAG GAACAACCTG 
GAAGCCTTAC CCCACAGCCA CAAAACCTCC CCAATGGAGG AACAATCTCT AAAACTTGAT 
TCTTTAGAGC CCAGGCTGCA GCGGGAGCTG GAGCGGCTAC AGGCAGCCCT GAGACAGACA 
GAAGCCAGGG AGATTGAGTG GAGGGAGAAG GCCCAGGACT TGGCACTCTC CCTAGCGCAG 
ACCAAGGCCA GTGTCAGCAG TCTGCAGGAG GTAGCCATGT TCCTACAAGC CTCTGTCCTG 
GAGCGGGACT CAGAACAGCA AAGGCTGCAG GATGAACTGG AGCTCACCAG ACGGGCTCTG 
GAGAAGGAGC GGCTACACAG CCCAGGTGCA ACCAGCACAG CAGAACTGGG GTCCAGAGGG 
GAGCAGGGTG TGCAGCTGGG AGAGGTCTCA GGAGTGGAGG CTGAGCCTAG TCCTGATGGA 
ATGGAGAAGC AGTCATGGAG ACAAAGGCTT GAACACCTGC AGCAAGCAGT GGCCCGGCTG 
GAGATTGACA GGAGCAGGCT GCAGCGCCAC AATGTCCAGC TGCGGAGTAC CTTGGAGCAG 
GTGGAGCGAG AACGGAGGAA GCTGAAGAGG GAGGCCATGC GTGCGGCCCA GGCAGGGTCC 
CTAGAGATCA GCAAGGCCAC GGCTTCTTCA CCCACACAGC AGGATGGGAG AGGACAGAAG 
AACTCAGATG CCAAGTGTGT GGCTGAACTG CAGAAAGAGG TGGTCCTGCT GCAAGCTCAG 
CTGACTTTGG AGCGGAAGCA GAAGCAGGAC TACATCACCC GCTCAGCACA GACCAGCCGT 
GAGCTAGCAG GCCTGCACCA CAGCCTCTCA CACTCACTTC TTGCCGTGGC CCAGGCCCCT 
GAGGCCACTG TCCTGGAGGC AGAGACCCGC AGGCTGGATG AGTCCCTGAC TCAAAGTCTG 
ACATCCCCAG GGCCAGTCCT GCTACACCCC AGCCCCAGCA CTACCCAAGC CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526821.1
CDS	120..6842
Translation	MELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_011528519.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528519.2

ORF Insert Sequence:

ATGGAGCTAA AAGCTGAGCA TGTGAGGCTT TCAGGGTCTC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACAGTC TCGGGAACCC AACGGATCTG GAAGAATGGA TGGGCGGGAG 
CCGGCCCAGC TGCTGCTGCT ACTAGCCAAG ACCCAGGAGC TGGAGAAGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC TGAACTTCAG 
GACCGGGTGA CCGAGCTCTC TGCTCTGTTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGA TAAAGGCTCT GAGAGAGACA GTGGAGATCC TGGAGACAAA TCACACAGAA 
TTAATGGAAC ATGAAGCATC TCTTAGTAGG AATGCGCAAG AGGAGAAGTT GTCTTTACAG 
CAGGTGATCA AGGATATAAC CCAGGTCATG GTGGAAGAAG GGGACAATAT AGCCCAAGGC 
TCTGGTCATG AGAACTCTTT GGAATTGGAC TCTAGTATCT TCTCCCAGTT TGATTACCAG 
GATGCAGACA AGGCTCTTAC TCTGGTGCGT TCAGTGCTGA CTCGGAGACG CCAGGCTGTG 
CAGGACCTAA GGCAGCAGCT TGCAGGCTGT CAAGAGGCTG TGAACTTGTT GCAACAGCAG 
CATGATCAGT GGGAGGAAGA GGGCAAAGCC TTGAGACAGC GGCTGCAGAA GCTCACTGGG 
GAGCGGGACA CTCTGGCAGG GCAGACTGTG GACCTCCAGG GAGAGGTGGA CTCTCTCAGC 
AAGGAGCGAG AGCTGCTGCA GAAGGCCAGG GAAGAGCTGC GGCAGCAGCT GGAGGTGCTA 
GAGCAGGAGG CATGGCGCCT GCGAAGGGTA AATGTGGAGC TTCAGCTGCA GGGGGACTCT 
GCCCAGGGCC AGAAGGAGGA ACAGCAGGAG GAGCTGCACC TGGCTGTCCG GGAGAGGGAG 
CGTCTTCAGG AGATGCTGAT GGGCCTGGAA GCCAAACAGT CAGAATCACT CAGTGAACTG 
ATCACTCTTC GGGAAGCCCT GGAGTCAAGT CACCTGGAAG GGGAGTTACT GAGGCAAGAG 
CAAACGGAAG TGACCGCAGC GCTGGCTAGG GCAGAGCAGT CAATTGCAGA GCTGTCGAGT 
TCTGAAAACA CCCTGAAGAC AGAAGTAGCT GATCTTCGGG CTGCAGCTGT CAAGCTCAGT 
GCCTTAAATG AGGCTTTGGC GTTAGATAAA GTTGGGCTGA ACCAGCAGCT TCTCCAGTTA 
GAGGAGGAGA ACCAGTCTGT GTGCAGCAGA ATGGAGGCCG CAGAGCAGGC GAGAAATGCT 
TTGCAGGTCG ACCTGGCGGA GGCAGAGAAG AGGAGGGAAG CCCTGTGGGA AAAGAACACT 
CACCTGGAGG CTCAGCTGCA GAAAGCTGAG GAGGCTGGGG CTGAGCTGCA GGCAGATCTC 
AGGGACATCC AAGAAGAGAA GGAAGAAATT CAAAAGAAAC TAAGTGAGTC ACGTCACCAG 
CAGGAGGCAG CCACGACTCA GCTGGAGCAG CTACATCAGG AGGCAAAGCG ACAGGAAGAA 
GTGCTTGCCA GGGCAGTCCA GGAGAAGGAG GCCCTAGTAC GAGAGAAAGC GGCTCTAGAG 
GTGCGGCTGC AGGCCGTGGA GCGTGACCGG CAGGACCTCG CTGAACAACT ACAGGGGCTC 
AGCTCAGCCA AGGAGCTACT GGAGAGCAGT CTGTTTGAAG CCCAACAACA AAATTCTGTG 
ATAGAGGTCA CCAAGGGGCA GCTGGAGGTC CAGATTCAAA CTGTCACTCA AGCCAAGGAA 
GTAATCCAAG GGGAAGTGAG GTGCCTGAAG CTGGAACTGG ACACTGAACG GAGTCAGGCA 
GAGCAGGAGC GGGATGCTGC AGCCAGACAG CTGGCCCAGG CTGAGCAAGA AGGGAAGACT 
GCCTTGGAGC AGCAGAAGGC AGCCCATGAG AAAGAGGTGA ACCAGCTCCG GGAGAAATGG 
GAGAAGGAGC GCTCCTGGCA CCAGCAGGAG CTGGCAAAGG CTCTGGAGAG CTTAGAAAGG 
GAAAAAATGG AGCTGGAAAT GAGGCTAAAG GAGCAGCAGA CAGAAATGGA GGCCATCCAG 
GCCCAGAGGG AAGAAGAACG GACCCAGGCA GAGAGTGCCC TATGCCAGAT GCAGCTGGAA 
ACAGAGAAGG AGAGAGTATC CCTCCTGGAG ACACTGCTGC AGACGCAGAA GGAGCTAGCA 
GATGCCAGCC AACAACTGGA ACGACTGAGG CAGGACATGA AAGTCCAGAA ATTAAAGGAG 
CAGGAGACCA CTGGGATACT ACAGACCCAG CTCCAGGAGG CTCAACGGGA GCTGAAGGAG 
GCAGCCCGGC AGCACAGAGA TGACCTTGCT GCCCTCCAAG AAGAGAGCAG CTCCCTGCTG 
CAGGATAAGA TGGACCTGCA GAAGCAGGTG GAGGACTTGA AGTCTCAGCT GGTGGCCCAG 
GATGACTCCC AGAGGCTGGT GGAGCAGGAG GTTCAGGAGA AGCTGAGAGA GACCCAGGAG 
TATAACCGAA TTCAGAAGGA GCTGGAGAGA GAGAAAGCCA GCCTGACTCT CTCACTGATG 
GAAAAGGAAC AGAGACTCCT TGTTTTACAA GAAGCTGACT CTATTCGACA ACAAGAGCTG 
AGTGCCCTGC GCCAGGACAT GCAGGAGGCC CAGGGAGAAC AGAAAGAGCT CAGTGCTCAG 
ATGGAATTAC TAAGGCAAGA GGTGAAGGAA AAGGAGGCTG ACTTTCTGGC CCAGGAAGCA 
CAGCTGCTGG AGGAGCTGGA GGCGTCTCAT ATCACGGAGC AGCAGCTGCG AGCCTCCTTG 
TGGGCCCAGG AAGCCAAGGC AGCCCAACTA CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG 
CTAGAAGCGC TGGCCGCAGA GCAGCAGCCC GGGAACCAGG CCCAGGCCCA GGCCCAGCTG 
GCCAGCCTCT ACTCTGCCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGTG GGGGAGACTC TGCTCCTTCC GTCTGGGGCC TTGAGCCAGA CCAGAATGGA 
GCTAGGAGCC TCTTTAAGAG AGGGCCCCTG CTGACTGCTC TCTCCGCTGA GGCAGTAGCA 
TCTGCCCTCC ACAAGCTTCA TCAAGACCTG TGGAAGACTC AACAGACCCG GGATGTTCTG 
AGGGATCAGG TCCAGAAACT GGAAGAGCGT CTAACTGATA CTGAGGCTGA GAAGAGCCAG 
GTCCACACAG AGTTGCAGGA TCTGCAGAGA CAGCTCTCCC AGAATCAGGA AGAGAAATCT 
AAGTGGGAAG GAAAGCAGAA CTCCCTAGAA TCTGAGCTGA TGGAACTACA TGAAACTATG 
GCATCCTTAC AGAGTCGCCT GCGGAGAGCA GAGCTACAGC GAATGGAAGC CCAGGGTGAG 
CGAGAGTTAC TTCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTGCAAGCA 
GCTGTCGTAG AAGCCAGGGC TCAGGCAAGT GCTGCTGGCA TCCTGGAAGA AGACCTGAGA 
ACGGCTCGCT CAGCACTGAA GCTGAAAAAT GAGGAAGTAG AGAGTGAGCG TGAGAGAGCC 
CAGGCTCTGC AAGAGCAGGG CGAACTGAAG GTGGCCCAAG GGAAGGCTCT GCAAGAGAAT 
TTGGCCCTCC TGACCCAGAC CCTAGCTGAA AGAGAAGAGG AGGTGGAGAC TCTGCGGGGA 
CAAATCCAGG AACTGGAGAA GCAACGGGAA ATGCAGAAGG CTGCTTTGGA ATTGCTGTCT 
CTGGACCTGA AGAAGAGGAA CCAAGAGGTA GATCTGCAGC AAGAACAGAT TCAGGAGCTA 
GAGAAGTGTA GGTCTGTTTT AGAGCATCTG CCCATGGCCG TCCAGGAGCG AGAGCAGAAG 
CTGACTGTGC AGAGGGAGCA GATCAGAGAG CTCGAGAAGG ATCGGGAGAC TCAGAGGAAC 
GTCTTGGAGC ATCAGCTTCT AGAACTTGAG AAGAAAGACC AAATGATTGA GTCCCAGAGA 
GGACAGGTTC AGGACCTGAA AAAGCAGTTG GTTACTCTGG AATGCCTGGC CCTGGAACTG 
GAGGAAAACC ATCACAAGAT GGAGTGCCAG CAAAAACTGA TCAAGGAGCT GGAGGGCCAG 
AGGGAAACCC AGAGAGTGGC TTTGACCCAC CTTACGCTGG ACCTAGAAGA AAGGAGCCAG 
GAGCTGCAGG CACAAAGCAG CCAGATCCAT GACCTGGAGA GCCACAGCAC CGTTCTGGCA 
AGAGAGCTGC AGGAGAGGGA CCAGGAGGTG AAGTCTCAGC GAGAACAGAT CGAGGAGCTG 
CAGAGGCAGA AAGAGCATCT GACTCAGGAT CTCGAGAGGA GAGACCAGGA GCTGATGCTG 
CAGAAGGAGA GGATTCAGGT TCTCGAGGAT CAGAGGACCC GGCAGACCAA GATCCTGGAG 
GAGGACCTGG AACAGATCAA GCTGTCCTTG AGAGAGCGAG GCCGGGAGCT GACCACTCAG 
AGGCAGCTGA TGCAGGAACG GGCAGAGGAA GGGAAGGGCC CAAGTAAAGC ACAGCGCGGG 
AGCCTAGAGC ACATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGTCAGCAG 
GAGCATATCC ATGAACTCCA GGAGCTCAAA GACCAGCTGG AGCAGCAGCT CCAGGGCCTG 
CACAGGAAGG TAGGTGAGAC CAGCCTCCTC CTGTCCCAGC GAGAGCAGGA AATAGTGGTC 
CTGCAGCAGC AACTGCAGGA AGCCAGGGAA CAAGGGGAGC TGAAGGAGCA GTCACTTCAG 
AGTCAACTGG ATGAGGCCCA GAGAGCCCTA GCCCAGAGGG ACCAGGAACT GGAGGCTCTG 
CAGCAAGAAC AGCAGCAGGC CCAGGGACAG GAGGAGAGGG TGAAGGAAAA GGCAGACGCC 
CTCCAGGGAG CTCTGGAGCA AGCCCATATG ACACTGAAGG AGCGTCATGG AGAGCTTCAG 
GACCACAAGG AACAGGCACG AAGGCTGGAG GAAGAGCTGG CAGTGGAGGG ACGGCGGGTC 
CAGGCCCTGG AGGAGGTGCT GGGAGACCTA AGGGCTGAGT CTCGGGAACA GGAGAAAGCT 
CTGTTGGCCC TCCAGCAGCA GTGTGCTGAG CAGGCACAGG AGCATGAGGT GGAGACCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCCCAG GCAGTGCTCA AGGAACGGGA CCAGGAGCTG 
GAAGCTCTGC GGGCAGAAAG TCAGTCCTCC CGGCATCAGG AGGAGGCTGC CCGGGCCCGG 
GCTGAGGCTC TGCAGGAGGC CCTTGGCAAG GCTCATGCTG CCCTGCAGGG GAAAGAGCAG 
CATCTCCTCG AGCAGGCAGA ATTGAGCCGC AGTCTGGAGG CCAGCACTGC AACCCTGCAA 
GCCTCCCTGG ATGCCTGCCA GGCACACAGT CGGCAGCTGG AGGAGGCTCT GAGGATACAA 
GAAGGTGAGA TCCAGGACCA GGATCTCCGA TACCAGGAGG ATGTGCAGCA GCTGCAGCAG 
GCACTTGCCC AGAGGGATGA AGAGCTGAGA CATCAGCAGG AACGGGAGCA GCTGCTGGAG 
AAGTCTCTGG CCCAGAGGGT CCAAGAGAAT ATGATCCAAG AGAAGCAGAA TCTGGGGCAA 
GAGAGAGAAG AGGAGGAGAT AAGGGGCCTT CATCAGAGTG TAAGGGAGCT ACAGCTGACT 
CTAGCCCAAA AGGAACAGGA GATTCTGGAG CTGAGGGAGA CCCAGCAAAG GAACAACCTG 
GAAGCCTTAC CCCACAGCCA CAAAACCTCC CCAATGGAGG AACAATCTCT AAAACTTGAT 
TCTTTAGAGC CCAGGCTGCA GCGGGAGCTG GAGCGGCTAC AGGCAGCCCT GAGACAGACA 
GAAGCCAGGG AGATTGAGTG GAGGGAGAAG GCCCAGGACT TGGCACTCTC CCTAGCGCAG 
ACCAAGGCCA GTGTCAGCAG TCTGCAGGAG GTAGCCATGT TCCTACAAGC CTCTGTCCTG 
GAGCGGGACT CAGAACAGCA AAGGCTGCAG GATGAACTGG AGCTCACCAG ACGGGCTCTG 
GAGAAGGAGC GGCTACACAG CCCAGGTGCA ACCAGCACAG CAGAACTGGG GTCCAGAGGG 
GAGCAGGGTG TGCAGCTGGG AGAGGTCTCA GGAGTGGAGG CTGAGCCTAG TCCTGATGGA 
ATGGAGAAGC AGTCATGGAG ACAAAGGCTT GAACACCTGC AGCAAGCAGT GGCCCGGCTG 
GAGATTGACA GGAGCAGGCT GCAGCGCCAC AATGTCCAGC TGCGGAGTAC CTTGGAGCAG 
GTGGAGCGAG AACGGAGGAA GCTGAAGAGG GAGGCCATGC GTGCGGCCCA GGCAGGGTCC 
CTAGAGATCA GCAAGGCCAC GGCTTCTTCA CCCACACAGC AGGATGGGAG AGGACAGAAG 
AACTCAGATG CCAAGTGTGT GGCTGAACTG CAGAAAGAGG TGGTCCTGCT GCAAGCTCAG 
CTGACTTTGG AGCGGAAGCA GAAGCAGGAC TACATCACCC GCTCAGCACA GACCAGCCGT 
GAGCTAGCAG GCCTGCACCA CAGCCTCTCA CACTCACTTC TTGCCGTGGC CCAGGCCCCT 
GAGGCCACTG TCCTGGAGGC AGAGACCCGC AGGCTGGATG AGTCCCTGAC TCAAAGTCTG 
ACATCCCCAG GGCCAGTCCT GCTACACCCC AGCCCCAGCA CTACCCAAGC CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_017027618.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883107.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X13, mRNA.

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CloneID	OHu92897
Clone ID Related Accession (Same CDS sequence)	XM_017027618.1
Accession Version	XM_017027618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTAT TGGCTTCAGG GTGCTTGACT TCAAGATCAA GCGAGGCCTG GAAGGGCCTG 
GGCCAGGGCT TTCCTTCAGC TCTTGGGCCC CCAAACACGT GTTCCAGCCT TGAGATGTGG 
GTGACCGAGC TCTCTGCTCT GTTGACCCAG TCTCAGAAGC AAAATGAAGA TTATGAAAAG 
ATGATAAAGG CTCTGAGAGA GACAGTGGAG ATCCTGGAGA CAAATCACAC AGAATTAATG 
GAACATGAAG CATCTCTTAG TAGGAATGCG CAAGAGGAGA AGTTGTCTTT ACAGCAGGTG 
ATCAAGGATA TAACCCAGGT CATGGTGGAA GAAGGGGACA ATATAGCCCA AGGCTCTGGT 
CATGAGAACT CTTTGGAATT GGACTCTAGT ATCTTCTCCC AGTTTGATTA CCAGGATGCA 
GACAAGGCTC TTACTCTGGT GCGTTCAGTG CTGACTCGGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTTGCAGG CTGTCAAGAG GCTGTGAACT TGTTGCAACA GCAGCATGAT 
CAGTGGGAGG AAGAGGGCAA AGCCTTGAGA CAGCGGCTGC AGAAGCTCAC TGGGGAGCGG 
GACACTCTGG CAGGGCAGAC TGTGGACCTC CAGGGAGAGG TGGACTCTCT CAGCAAGGAG 
CGAGAGCTGC TGCAGAAGGC CAGGGAAGAG CTGCGGCAGC AGCTGGAGGT GCTAGAGCAG 
GAGGCATGGC GCCTGCGAAG GGTAAATGTG GAGCTTCAGC TGCAGGGGGA CTCTGCCCAG 
GGCCAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCTG TCCGGGAGAG GGAGCGTCTT 
CAGGAGATGC TGATGGGCCT GGAAGCCAAA CAGTCAGAAT CACTCAGTGA ACTGATCACT 
CTTCGGGAAG CCCTGGAGTC AAGTCACCTG GAAGGGGAGT TACTGAGGCA AGAGCAAACG 
GAAGTGACCG CAGCGCTGGC TAGGGCAGAG CAGTCAATTG CAGAGCTGTC GAGTTCTGAA 
AACACCCTGA AGACAGAAGT AGCTGATCTT CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA 
AATGAGGCTT TGGCGTTAGA TAAAGTTGGG CTGAACCAGC AGCTTCTCCA GTTAGAGGAG 
GAGAACCAGT CTGTGTGCAG CAGAATGGAG GCCGCAGAGC AGGCGAGAAA TGCTTTGCAG 
GTCGACCTGG CGGAGGCAGA GAAGAGGAGG GAAGCCCTGT GGGAAAAGAA CACTCACCTG 
GAGGCTCAGC TGCAGAAAGC TGAGGAGGCT GGGGCTGAGC TGCAGGCAGA TCTCAGGGAC 
ATCCAAGAAG AGAAGGAAGA AATTCAAAAG AAACTAAGTG AGTCACGTCA CCAGCAGGAG 
GCAGCCACGA CTCAGCTGGA GCAGCTACAT CAGGAGGCAA AGCGACAGGA AGAAGTGCTT 
GCCAGGGCAG TCCAGGAGAA GGAGGCCCTA GTACGAGAGA AAGCGGCTCT AGAGGTGCGG 
CTGCAGGCCG TGGAGCGTGA CCGGCAGGAC CTCGCTGAAC AACTACAGGG GCTCAGCTCA 
GCCAAGGAGC TACTGGAGAG CAGTCTGTTT GAAGCCCAAC AACAAAATTC TGTGATAGAG 
GTCACCAAGG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CTCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA CTGGACACTG AACGGAGTCA GGCAGAGCAG 
GAGCGGGATG CTGCAGCCAG ACAGCTGGCC CAGGCTGAGC AAGAAGGGAA GACTGCCTTG 
GAGCAGCAGA AGGCAGCCCA TGAGAAAGAG GTGAACCAGC TCCGGGAGAA ATGGGAGAAG 
GAGCGCTCCT GGCACCAGCA GGAGCTGGCA AAGGCTCTGG AGAGCTTAGA AAGGGAAAAA 
ATGGAGCTGG AAATGAGGCT AAAGGAGCAG CAGACAGAAA TGGAGGCCAT CCAGGCCCAG 
AGGGAAGAAG AACGGACCCA GGCAGAGAGT GCCCTATGCC AGATGCAGCT GGAAACAGAG 
AAGGAGAGAG TATCCCTCCT GGAGACACTG CTGCAGACGC AGAAGGAGCT AGCAGATGCC 
AGCCAACAAC TGGAACGACT GAGGCAGGAC ATGAAAGTCC AGAAATTAAA GGAGCAGGAG 
ACCACTGGGA TACTACAGAC CCAGCTCCAG GAGGCTCAAC GGGAGCTGAA GGAGGCAGCC 
CGGCAGCACA GAGATGACCT TGCTGCCCTC CAAGAAGAGA GCAGCTCCCT GCTGCAGGAT 
AAGATGGACC TGCAGAAGCA GGTGGAGGAC TTGAAGTCTC AGCTGGTGGC CCAGGATGAC 
TCCCAGAGGC TGGTGGAGCA GGAGGTTCAG GAGAAGCTGA GAGAGACCCA GGAGTATAAC 
CGAATTCAGA AGGAGCTGGA GAGAGAGAAA GCCAGCCTGA CTCTCTCACT GATGGAAAAG 
GAACAGAGAC TCCTTGTTTT ACAAGAAGCT GACTCTATTC GACAACAAGA GCTGAGTGCC 
CTGCGCCAGG ACATGCAGGA GGCCCAGGGA GAACAGAAAG AGCTCAGTGC TCAGATGGAA 
TTACTAAGGC AAGAGGTGAA GGAAAAGGAG GCTGACTTTC TGGCCCAGGA AGCACAGCTG 
CTGGAGGAGC TGGAGGCGTC TCATATCACG GAGCAGCAGC TGCGAGCCTC CTTGTGGGCC 
CAGGAAGCCA AGGCAGCCCA ACTACAGCTG CGACTGCGCA GCACAGAGAG CCAGCTAGAA 
GCGCTGGCCG CAGAGCAGCA GCCCGGGAAC CAGGCCCAGG CCCAGGCCCA GCTGGCCAGC 
CTCTACTCTG CCCTGCAGCA GGCCCTGGGG TCTGTTTGTG AGAGCAGGCC TGAGCTGAGT 
GGTGGGGGAG ACTCTGCTCC TTCCGTCTGG GGCCTTGAGC CAGACCAGAA TGGAGCTAGG 
AGCCTCTTTA AGAGAGGGCC CCTGCTGACT GCTCTCTCCG CTGAGGCAGT AGCATCTGCC 
CTCCACAAGC TTCATCAAGA CCTGTGGAAG ACTCAACAGA CCCGGGATGT TCTGAGGGAT 
CAGGTCCAGA AACTGGAAGA GCGTCTAACT GATACTGAGG CTGAGAAGAG CCAGGTCCAC 
ACAGAGTTGC AGGATCTGCA GAGACAGCTC TCCCAGAATC AGGAAGAGAA ATCTAAGTGG 
GAAGGAAAGC AGAACTCCCT AGAATCTGAG CTGATGGAAC TACATGAAAC TATGGCATCC 
TTACAGAGTC GCCTGCGGAG AGCAGAGCTA CAGCGAATGG AAGCCCAGGG TGAGCGAGAG 
TTACTTCAGG CAGCCAAGGA GAACCTGACA GCCCAGGTGG AACACCTGCA AGCAGCTGTC 
GTAGAAGCCA GGGCTCAGGC AAGTGCTGCT GGCATCCTGG AAGAAGACCT GAGAACGGCT 
CGCTCAGCAC TGAAGCTGAA AAATGAGGAA GTAGAGAGTG AGCGTGAGAG AGCCCAGGCT 
CTGCAAGAGC AGGGCGAACT GAAGGTGGCC CAAGGGAAGG CTCTGCAAGA GAATTTGGCC 
CTCCTGACCC AGACCCTAGC TGAAAGAGAA GAGGAGGTGG AGACTCTGCG GGGACAAATC 
CAGGAACTGG AGAAGCAACG GGAAATGCAG AAGGCTGCTT TGGAATTGCT GTCTCTGGAC 
CTGAAGAAGA GGAACCAAGA GGTAGATCTG CAGCAAGAAC AGATTCAGGA GCTAGAGAAG 
TGTAGGTCTG TTTTAGAGCA TCTGCCCATG GCCGTCCAGG AGCGAGAGCA GAAGCTGACT 
GTGCAGAGGG AGCAGATCAG AGAGCTCGAG AAGGATCGGG AGACTCAGAG GAACGTCTTG 
GAGCATCAGC TTCTAGAACT TGAGAAGAAA GACCAAATGA TTGAGTCCCA GAGAGGACAG 
GTTCAGGACC TGAAAAAGCA GTTGGTTACT CTGGAATGCC TGGCCCTGGA ACTGGAGGAA 
AACCATCACA AGATGGAGTG CCAGCAAAAA CTGATCAAGG AGCTGGAGGG CCAGAGGGAA 
ACCCAGAGAG TGGCTTTGAC CCACCTTACG CTGGACCTAG AAGAAAGGAG CCAGGAGCTG 
CAGGCACAAA GCAGCCAGAT CCATGACCTG GAGAGCCACA GCACCGTTCT GGCAAGAGAG 
CTGCAGGAGA GGGACCAGGA GGTGAAGTCT CAGCGAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAAGAGC ATCTGACTCA GGATCTCGAG AGGAGAGACC AGGAGCTGAT GCTGCAGAAG 
GAGAGGATTC AGGTTCTCGA GGATCAGAGG ACCCGGCAGA CCAAGATCCT GGAGGAGGAC 
CTGGAACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGACCAC TCAGAGGCAG 
CTGATGCAGG AACGGGCAGA GGAAGGGAAG GGCCCAAGTA AAGCACAGCG CGGGAGCCTA 
GAGCACATGA AGCTGATCCT GCGTGATAAG GAGAAGGAGG TGGAATGTCA GCAGGAGCAT 
ATCCATGAAC TCCAGGAGCT CAAAGACCAG CTGGAGCAGC AGCTCCAGGG CCTGCACAGG 
AAGGTAGGTG AGACCAGCCT CCTCCTGTCC CAGCGAGAGC AGGAAATAGT GGTCCTGCAG 
CAGCAACTGC AGGAAGCCAG GGAACAAGGG GAGCTGAAGG AGCAGTCACT TCAGAGTCAA 
CTGGATGAGG CCCAGAGAGC CCTAGCCCAG AGGGACCAGG AACTGGAGGC TCTGCAGCAA 
GAACAGCAGC AGGCCCAGGG ACAGGAGGAG AGGGTGAAGG AAAAGGCAGA CGCCCTCCAG 
GGAGCTCTGG AGCAAGCCCA TATGACACTG AAGGAGCGTC ATGGAGAGCT TCAGGACCAC 
AAGGAACAGG CACGAAGGCT GGAGGAAGAG CTGGCAGTGG AGGGACGGCG GGTCCAGGCC 
CTGGAGGAGG TGCTGGGAGA CCTAAGGGCT GAGTCTCGGG AACAGGAGAA AGCTCTGTTG 
GCCCTCCAGC AGCAGTGTGC TGAGCAGGCA CAGGAGCATG AGGTGGAGAC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC CCAGGCAGTG CTCAAGGAAC GGGACCAGGA GCTGGAAGCT 
CTGCGGGCAG AAAGTCAGTC CTCCCGGCAT CAGGAGGAGG CTGCCCGGGC CCGGGCTGAG 
GCTCTGCAGG AGGCCCTTGG CAAGGCTCAT GCTGCCCTGC AGGGGAAAGA GCAGCATCTC 
CTCGAGCAGG CAGAATTGAG CCGCAGTCTG GAGGCCAGCA CTGCAACCCT GCAAGCCTCC 
CTGGATGCCT GCCAGGCACA CAGTCGGCAG CTGGAGGAGG CTCTGAGGAT ACAAGAAGGT 
GAGATCCAGG ACCAGGATCT CCGATACCAG GAGGATGTGC AGCAGCTGCA GCAGGCACTT 
GCCCAGAGGG ATGAAGAGCT GAGACATCAG CAGGAACGGG AGCAGCTGCT GGAGAAGTCT 
CTGGCCCAGA GGGTCCAAGA GAATATGATC CAAGAGAAGC AGAATCTGGG GCAAGAGAGA 
GAAGAGGAGG AGATAAGGGG CCTTCATCAG AGTGTAAGGG AGCTACAGCT GACTCTAGCC 
CAAAAGGAAC AGGAGATTCT GGAGCTGAGG GAGACCCAGC AAAGGAACAA CCTGGAAGCC 
TTACCCCACA GCCACAAAAC CTCCCCAATG GAGGAACAAT CTCTAAAACT TGATTCTTTA 
GAGCCCAGGC TGCAGCGGGA GCTGGAGCGG CTACAGGCAG CCCTGAGACA GACAGAAGCC 
AGGGAGATTG AGTGGAGGGA GAAGGCCCAG GACTTGGCAC TCTCCCTAGC GCAGACCAAG 
GCCAGTGTCA GCAGTCTGCA GGAGGTAGCC ATGTTCCTAC AAGCCTCTGT CCTGGAGCGG 
GACTCAGAAC AGCAAAGGCT GCAGGATGAA CTGGAGCTCA CCAGACGGGC TCTGGAGAAG 
GAGCGGCTAC ACAGCCCAGG TGCAACCAGC ACAGCAGAAC TGGGGTCCAG AGGGGAGCAG 
GGTGTGCAGC TGGGAGAGGT CTCAGGAGTG GAGGCTGAGC CTAGTCCTGA TGGAATGGAG 
AAGCAGTCAT GGAGACAAAG GCTTGAACAC CTGCAGCAAG CAGTGGCCCG GCTGGAGATT 
GACAGGAGCA GGCTGCAGCG CCACAATGTC CAGCTGCGGA GTACCTTGGA GCAGGTGGAG 
CGAGAACGGA GGAAGCTGAA GAGGGAGGCC ATGCGTGCGG CCCAGGCAGG GTCCCTAGAG 
ATCAGCAAGG CCACGGCTTC TTCACCCACA CAGCAGGATG GGAGAGGACA GAAGAACTCA 
GATGCCAAGT GTGTGGCTGA ACTGCAGAAA GAGGTGGTCC TGCTGCAAGC TCAGCTGACT 
TTGGAGCGGA AGCAGAAGCA GGACTACATC ACCCGCTCAG CACAGACCAG CCGTGAGCTA 
GCAGGCCTGC ACCACAGCCT CTCACACTCA CTTCTTGCCG TGGCCCAGGC CCCTGAGGCC 
ACTGTCCTGG AGGCAGAGAC CCGCAGGCTG GATGAGTCCC TGACTCAAAG TCTGACATCC 
CCAGGGCCAG TCCTGCTACA CCCCAGCCCC AGCACTACCC AAGCCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883107.1
CDS	233..6829
Translation	MFLLASGCLTSRSSEAWKGLGQGFPSALGPPNTCSSLEMWVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_017027618.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027618.1

ORF Insert Sequence:

ATGTTCTTAT TGGCTTCAGG GTGCTTGACT TCAAGATCAA GCGAGGCCTG GAAGGGCCTG 
GGCCAGGGCT TTCCTTCAGC TCTTGGGCCC CCAAACACGT GTTCCAGCCT TGAGATGTGG 
GTGACCGAGC TCTCTGCTCT GTTGACCCAG TCTCAGAAGC AAAATGAAGA TTATGAAAAG 
ATGATAAAGG CTCTGAGAGA GACAGTGGAG ATCCTGGAGA CAAATCACAC AGAATTAATG 
GAACATGAAG CATCTCTTAG TAGGAATGCG CAAGAGGAGA AGTTGTCTTT ACAGCAGGTG 
ATCAAGGATA TAACCCAGGT CATGGTGGAA GAAGGGGACA ATATAGCCCA AGGCTCTGGT 
CATGAGAACT CTTTGGAATT GGACTCTAGT ATCTTCTCCC AGTTTGATTA CCAGGATGCA 
GACAAGGCTC TTACTCTGGT GCGTTCAGTG CTGACTCGGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTTGCAGG CTGTCAAGAG GCTGTGAACT TGTTGCAACA GCAGCATGAT 
CAGTGGGAGG AAGAGGGCAA AGCCTTGAGA CAGCGGCTGC AGAAGCTCAC TGGGGAGCGG 
GACACTCTGG CAGGGCAGAC TGTGGACCTC CAGGGAGAGG TGGACTCTCT CAGCAAGGAG 
CGAGAGCTGC TGCAGAAGGC CAGGGAAGAG CTGCGGCAGC AGCTGGAGGT GCTAGAGCAG 
GAGGCATGGC GCCTGCGAAG GGTAAATGTG GAGCTTCAGC TGCAGGGGGA CTCTGCCCAG 
GGCCAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCTG TCCGGGAGAG GGAGCGTCTT 
CAGGAGATGC TGATGGGCCT GGAAGCCAAA CAGTCAGAAT CACTCAGTGA ACTGATCACT 
CTTCGGGAAG CCCTGGAGTC AAGTCACCTG GAAGGGGAGT TACTGAGGCA AGAGCAAACG 
GAAGTGACCG CAGCGCTGGC TAGGGCAGAG CAGTCAATTG CAGAGCTGTC GAGTTCTGAA 
AACACCCTGA AGACAGAAGT AGCTGATCTT CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA 
AATGAGGCTT TGGCGTTAGA TAAAGTTGGG CTGAACCAGC AGCTTCTCCA GTTAGAGGAG 
GAGAACCAGT CTGTGTGCAG CAGAATGGAG GCCGCAGAGC AGGCGAGAAA TGCTTTGCAG 
GTCGACCTGG CGGAGGCAGA GAAGAGGAGG GAAGCCCTGT GGGAAAAGAA CACTCACCTG 
GAGGCTCAGC TGCAGAAAGC TGAGGAGGCT GGGGCTGAGC TGCAGGCAGA TCTCAGGGAC 
ATCCAAGAAG AGAAGGAAGA AATTCAAAAG AAACTAAGTG AGTCACGTCA CCAGCAGGAG 
GCAGCCACGA CTCAGCTGGA GCAGCTACAT CAGGAGGCAA AGCGACAGGA AGAAGTGCTT 
GCCAGGGCAG TCCAGGAGAA GGAGGCCCTA GTACGAGAGA AAGCGGCTCT AGAGGTGCGG 
CTGCAGGCCG TGGAGCGTGA CCGGCAGGAC CTCGCTGAAC AACTACAGGG GCTCAGCTCA 
GCCAAGGAGC TACTGGAGAG CAGTCTGTTT GAAGCCCAAC AACAAAATTC TGTGATAGAG 
GTCACCAAGG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CTCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAGGTGCCT GAAGCTGGAA CTGGACACTG AACGGAGTCA GGCAGAGCAG 
GAGCGGGATG CTGCAGCCAG ACAGCTGGCC CAGGCTGAGC AAGAAGGGAA GACTGCCTTG 
GAGCAGCAGA AGGCAGCCCA TGAGAAAGAG GTGAACCAGC TCCGGGAGAA ATGGGAGAAG 
GAGCGCTCCT GGCACCAGCA GGAGCTGGCA AAGGCTCTGG AGAGCTTAGA AAGGGAAAAA 
ATGGAGCTGG AAATGAGGCT AAAGGAGCAG CAGACAGAAA TGGAGGCCAT CCAGGCCCAG 
AGGGAAGAAG AACGGACCCA GGCAGAGAGT GCCCTATGCC AGATGCAGCT GGAAACAGAG 
AAGGAGAGAG TATCCCTCCT GGAGACACTG CTGCAGACGC AGAAGGAGCT AGCAGATGCC 
AGCCAACAAC TGGAACGACT GAGGCAGGAC ATGAAAGTCC AGAAATTAAA GGAGCAGGAG 
ACCACTGGGA TACTACAGAC CCAGCTCCAG GAGGCTCAAC GGGAGCTGAA GGAGGCAGCC 
CGGCAGCACA GAGATGACCT TGCTGCCCTC CAAGAAGAGA GCAGCTCCCT GCTGCAGGAT 
AAGATGGACC TGCAGAAGCA GGTGGAGGAC TTGAAGTCTC AGCTGGTGGC CCAGGATGAC 
TCCCAGAGGC TGGTGGAGCA GGAGGTTCAG GAGAAGCTGA GAGAGACCCA GGAGTATAAC 
CGAATTCAGA AGGAGCTGGA GAGAGAGAAA GCCAGCCTGA CTCTCTCACT GATGGAAAAG 
GAACAGAGAC TCCTTGTTTT ACAAGAAGCT GACTCTATTC GACAACAAGA GCTGAGTGCC 
CTGCGCCAGG ACATGCAGGA GGCCCAGGGA GAACAGAAAG AGCTCAGTGC TCAGATGGAA 
TTACTAAGGC AAGAGGTGAA GGAAAAGGAG GCTGACTTTC TGGCCCAGGA AGCACAGCTG 
CTGGAGGAGC TGGAGGCGTC TCATATCACG GAGCAGCAGC TGCGAGCCTC CTTGTGGGCC 
CAGGAAGCCA AGGCAGCCCA ACTACAGCTG CGACTGCGCA GCACAGAGAG CCAGCTAGAA 
GCGCTGGCCG CAGAGCAGCA GCCCGGGAAC CAGGCCCAGG CCCAGGCCCA GCTGGCCAGC 
CTCTACTCTG CCCTGCAGCA GGCCCTGGGG TCTGTTTGTG AGAGCAGGCC TGAGCTGAGT 
GGTGGGGGAG ACTCTGCTCC TTCCGTCTGG GGCCTTGAGC CAGACCAGAA TGGAGCTAGG 
AGCCTCTTTA AGAGAGGGCC CCTGCTGACT GCTCTCTCCG CTGAGGCAGT AGCATCTGCC 
CTCCACAAGC TTCATCAAGA CCTGTGGAAG ACTCAACAGA CCCGGGATGT TCTGAGGGAT 
CAGGTCCAGA AACTGGAAGA GCGTCTAACT GATACTGAGG CTGAGAAGAG CCAGGTCCAC 
ACAGAGTTGC AGGATCTGCA GAGACAGCTC TCCCAGAATC AGGAAGAGAA ATCTAAGTGG 
GAAGGAAAGC AGAACTCCCT AGAATCTGAG CTGATGGAAC TACATGAAAC TATGGCATCC 
TTACAGAGTC GCCTGCGGAG AGCAGAGCTA CAGCGAATGG AAGCCCAGGG TGAGCGAGAG 
TTACTTCAGG CAGCCAAGGA GAACCTGACA GCCCAGGTGG AACACCTGCA AGCAGCTGTC 
GTAGAAGCCA GGGCTCAGGC AAGTGCTGCT GGCATCCTGG AAGAAGACCT GAGAACGGCT 
CGCTCAGCAC TGAAGCTGAA AAATGAGGAA GTAGAGAGTG AGCGTGAGAG AGCCCAGGCT 
CTGCAAGAGC AGGGCGAACT GAAGGTGGCC CAAGGGAAGG CTCTGCAAGA GAATTTGGCC 
CTCCTGACCC AGACCCTAGC TGAAAGAGAA GAGGAGGTGG AGACTCTGCG GGGACAAATC 
CAGGAACTGG AGAAGCAACG GGAAATGCAG AAGGCTGCTT TGGAATTGCT GTCTCTGGAC 
CTGAAGAAGA GGAACCAAGA GGTAGATCTG CAGCAAGAAC AGATTCAGGA GCTAGAGAAG 
TGTAGGTCTG TTTTAGAGCA TCTGCCCATG GCCGTCCAGG AGCGAGAGCA GAAGCTGACT 
GTGCAGAGGG AGCAGATCAG AGAGCTCGAG AAGGATCGGG AGACTCAGAG GAACGTCTTG 
GAGCATCAGC TTCTAGAACT TGAGAAGAAA GACCAAATGA TTGAGTCCCA GAGAGGACAG 
GTTCAGGACC TGAAAAAGCA GTTGGTTACT CTGGAATGCC TGGCCCTGGA ACTGGAGGAA 
AACCATCACA AGATGGAGTG CCAGCAAAAA CTGATCAAGG AGCTGGAGGG CCAGAGGGAA 
ACCCAGAGAG TGGCTTTGAC CCACCTTACG CTGGACCTAG AAGAAAGGAG CCAGGAGCTG 
CAGGCACAAA GCAGCCAGAT CCATGACCTG GAGAGCCACA GCACCGTTCT GGCAAGAGAG 
CTGCAGGAGA GGGACCAGGA GGTGAAGTCT CAGCGAGAAC AGATCGAGGA GCTGCAGAGG 
CAGAAAGAGC ATCTGACTCA GGATCTCGAG AGGAGAGACC AGGAGCTGAT GCTGCAGAAG 
GAGAGGATTC AGGTTCTCGA GGATCAGAGG ACCCGGCAGA CCAAGATCCT GGAGGAGGAC 
CTGGAACAGA TCAAGCTGTC CTTGAGAGAG CGAGGCCGGG AGCTGACCAC TCAGAGGCAG 
CTGATGCAGG AACGGGCAGA GGAAGGGAAG GGCCCAAGTA AAGCACAGCG CGGGAGCCTA 
GAGCACATGA AGCTGATCCT GCGTGATAAG GAGAAGGAGG TGGAATGTCA GCAGGAGCAT 
ATCCATGAAC TCCAGGAGCT CAAAGACCAG CTGGAGCAGC AGCTCCAGGG CCTGCACAGG 
AAGGTAGGTG AGACCAGCCT CCTCCTGTCC CAGCGAGAGC AGGAAATAGT GGTCCTGCAG 
CAGCAACTGC AGGAAGCCAG GGAACAAGGG GAGCTGAAGG AGCAGTCACT TCAGAGTCAA 
CTGGATGAGG CCCAGAGAGC CCTAGCCCAG AGGGACCAGG AACTGGAGGC TCTGCAGCAA 
GAACAGCAGC AGGCCCAGGG ACAGGAGGAG AGGGTGAAGG AAAAGGCAGA CGCCCTCCAG 
GGAGCTCTGG AGCAAGCCCA TATGACACTG AAGGAGCGTC ATGGAGAGCT TCAGGACCAC 
AAGGAACAGG CACGAAGGCT GGAGGAAGAG CTGGCAGTGG AGGGACGGCG GGTCCAGGCC 
CTGGAGGAGG TGCTGGGAGA CCTAAGGGCT GAGTCTCGGG AACAGGAGAA AGCTCTGTTG 
GCCCTCCAGC AGCAGTGTGC TGAGCAGGCA CAGGAGCATG AGGTGGAGAC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC CCAGGCAGTG CTCAAGGAAC GGGACCAGGA GCTGGAAGCT 
CTGCGGGCAG AAAGTCAGTC CTCCCGGCAT CAGGAGGAGG CTGCCCGGGC CCGGGCTGAG 
GCTCTGCAGG AGGCCCTTGG CAAGGCTCAT GCTGCCCTGC AGGGGAAAGA GCAGCATCTC 
CTCGAGCAGG CAGAATTGAG CCGCAGTCTG GAGGCCAGCA CTGCAACCCT GCAAGCCTCC 
CTGGATGCCT GCCAGGCACA CAGTCGGCAG CTGGAGGAGG CTCTGAGGAT ACAAGAAGGT 
GAGATCCAGG ACCAGGATCT CCGATACCAG GAGGATGTGC AGCAGCTGCA GCAGGCACTT 
GCCCAGAGGG ATGAAGAGCT GAGACATCAG CAGGAACGGG AGCAGCTGCT GGAGAAGTCT 
CTGGCCCAGA GGGTCCAAGA GAATATGATC CAAGAGAAGC AGAATCTGGG GCAAGAGAGA 
GAAGAGGAGG AGATAAGGGG CCTTCATCAG AGTGTAAGGG AGCTACAGCT GACTCTAGCC 
CAAAAGGAAC AGGAGATTCT GGAGCTGAGG GAGACCCAGC AAAGGAACAA CCTGGAAGCC 
TTACCCCACA GCCACAAAAC CTCCCCAATG GAGGAACAAT CTCTAAAACT TGATTCTTTA 
GAGCCCAGGC TGCAGCGGGA GCTGGAGCGG CTACAGGCAG CCCTGAGACA GACAGAAGCC 
AGGGAGATTG AGTGGAGGGA GAAGGCCCAG GACTTGGCAC TCTCCCTAGC GCAGACCAAG 
GCCAGTGTCA GCAGTCTGCA GGAGGTAGCC ATGTTCCTAC AAGCCTCTGT CCTGGAGCGG 
GACTCAGAAC AGCAAAGGCT GCAGGATGAA CTGGAGCTCA CCAGACGGGC TCTGGAGAAG 
GAGCGGCTAC ACAGCCCAGG TGCAACCAGC ACAGCAGAAC TGGGGTCCAG AGGGGAGCAG 
GGTGTGCAGC TGGGAGAGGT CTCAGGAGTG GAGGCTGAGC CTAGTCCTGA TGGAATGGAG 
AAGCAGTCAT GGAGACAAAG GCTTGAACAC CTGCAGCAAG CAGTGGCCCG GCTGGAGATT 
GACAGGAGCA GGCTGCAGCG CCACAATGTC CAGCTGCGGA GTACCTTGGA GCAGGTGGAG 
CGAGAACGGA GGAAGCTGAA GAGGGAGGCC ATGCGTGCGG CCCAGGCAGG GTCCCTAGAG 
ATCAGCAAGG CCACGGCTTC TTCACCCACA CAGCAGGATG GGAGAGGACA GAAGAACTCA 
GATGCCAAGT GTGTGGCTGA ACTGCAGAAA GAGGTGGTCC TGCTGCAAGC TCAGCTGACT 
TTGGAGCGGA AGCAGAAGCA GGACTACATC ACCCGCTCAG CACAGACCAG CCGTGAGCTA 
GCAGGCCTGC ACCACAGCCT CTCACACTCA CTTCTTGCCG TGGCCCAGGC CCCTGAGGCC 
ACTGTCCTGG AGGCAGAGAC CCGCAGGCTG GATGAGTCCC TGACTCAAAG TCTGACATCC 
CCAGGGCCAG TCCTGCTACA CCCCAGCCCC AGCACTACCC AAGCCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_017027619.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883108.1 Homo sapiens centrosomal protein 250 (CEP250), transcript variant X14, mRNA.

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CloneID	OHu92898
Clone ID Related Accession (Same CDS sequence)	XM_017027619.1
Accession Version	XM_017027619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosome-associated protein CEP250 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTG CGAAGGGTAA ATGTGGAGCT TCAGCTGCAG GGGGACTCTG CCCAGGGCCA 
GAAGGAGGAA CAGCAGGAGG AGCTGCACCT GGCTGTCCGG GAGAGGGAGC GTCTGGTTGC 
TGGGAAGATG AGAGCAATGT TTTGGAAAAC GCCAGCATAG AACCTGGCAT ATGTCAGGAG 
ATGCTGATGG GCCTGGAAGC CAAACAGTCA GAATCACTCA GTGAACTGAT CACTCTTCGG 
GAAGCCCTGG AGTCAAGTCA CCTGGAAGGG GAGTTACTGA GGCAAGAGCA AACGGAAGTG 
ACCGCAGCGC TGGCTAGGGC AGAGCAGTCA ATTGCAGAGC TGTCGAGTTC TGAAAACACC 
CTGAAGACAG AAGTAGCTGA TCTTCGGGCT GCAGCTGTCA AGCTCAGTGC CTTAAATGAG 
GCTTTGGCGT TAGATAAAGT TGGGCTGAAC CAGCAGCTTC TCCAGTTAGA GGAGGAGAAC 
CAGTCTGTGT GCAGCAGAAT GGAGGCCGCA GAGCAGGCGA GAAATGCTTT GCAGGTCGAC 
CTGGCGGAGG CAGAGAAGAG GAGGGAAGCC CTGTGGGAAA AGAACACTCA CCTGGAGGCT 
CAGCTGCAGA AAGCTGAGGA GGCTGGGGCT GAGCTGCAGG CAGATCTCAG GGACATCCAA 
GAAGAGAAGG AAGAAATTCA AAAGAAACTA AGTGAGTCAC GTCACCAGCA GGAGGCAGCC 
ACGACTCAGC TGGAGCAGCT ACATCAGGAG GCAAAGCGAC AGGAAGAAGT GCTTGCCAGG 
GCAGTCCAGG AGAAGGAGGC CCTAGTACGA GAGAAAGCGG CTCTAGAGGT GCGGCTGCAG 
GCCGTGGAGC GTGACCGGCA GGACCTCGCT GAACAACTAC AGGGGCTCAG CTCAGCCAAG 
GAGCTACTGG AGAGCAGTCT GTTTGAAGCC CAACAACAAA ATTCTGTGAT AGAGGTCACC 
AAGGGGCAGC TGGAGGTCCA GATTCAAACT GTCACTCAAG CCAAGGAAGT AATCCAAGGG 
GAAGTGAGGT GCCTGAAGCT GGAACTGGAC ACTGAACGGA GTCAGGCAGA GCAGGAGCGG 
GATGCTGCAG CCAGACAGCT GGCCCAGGCT GAGCAAGAAG GGAAGACTGC CTTGGAGCAG 
CAGAAGGCAG CCCATGAGAA AGAGGTGAAC CAGCTCCGGG AGAAATGGGA GAAGGAGCGC 
TCCTGGCACC AGCAGGAGCT GGCAAAGGCT CTGGAGAGCT TAGAAAGGGA AAAAATGGAG 
CTGGAAATGA GGCTAAAGGA GCAGCAGACA GAAATGGAGG CCATCCAGGC CCAGAGGGAA 
GAAGAACGGA CCCAGGCAGA GAGTGCCCTA TGCCAGATGC AGCTGGAAAC AGAGAAGGAG 
AGAGTATCCC TCCTGGAGAC ACTGCTGCAG ACGCAGAAGG AGCTAGCAGA TGCCAGCCAA 
CAACTGGAAC GACTGAGGCA GGACATGAAA GTCCAGAAAT TAAAGGAGCA GGAGACCACT 
GGGATACTAC AGACCCAGCT CCAGGAGGCT CAACGGGAGC TGAAGGAGGC AGCCCGGCAG 
CACAGAGATG ACCTTGCTGC CCTCCAAGAA GAGAGCAGCT CCCTGCTGCA GGATAAGATG 
GACCTGCAGA AGCAGGTGGA GGACTTGAAG TCTCAGCTGG TGGCCCAGGA TGACTCCCAG 
AGGCTGGTGG AGCAGGAGGT TCAGGAGAAG CTGAGAGAGA CCCAGGAGTA TAACCGAATT 
CAGAAGGAGC TGGAGAGAGA GAAAGCCAGC CTGACTCTCT CACTGATGGA AAAGGAACAG 
AGACTCCTTG TTTTACAAGA AGCTGACTCT ATTCGACAAC AAGAGCTGAG TGCCCTGCGC 
CAGGACATGC AGGAGGCCCA GGGAGAACAG AAAGAGCTCA GTGCTCAGAT GGAATTACTA 
AGGCAAGAGG TGAAGGAAAA GGAGGCTGAC TTTCTGGCCC AGGAAGCACA GCTGCTGGAG 
GAGCTGGAGG CGTCTCATAT CACGGAGCAG CAGCTGCGAG CCTCCTTGTG GGCCCAGGAA 
GCCAAGGCAG CCCAACTACA GCTGCGACTG CGCAGCACAG AGAGCCAGCT AGAAGCGCTG 
GCCGCAGAGC AGCAGCCCGG GAACCAGGCC CAGGCCCAGG CCCAGCTGGC CAGCCTCTAC 
TCTGCCCTGC AGCAGGCCCT GGGGTCTGTT TGTGAGAGCA GGCCTGAGCT GAGTGGTGGG 
GGAGACTCTG CTCCTTCCGT CTGGGGCCTT GAGCCAGACC AGAATGGAGC TAGGAGCCTC 
TTTAAGAGAG GGCCCCTGCT GACTGCTCTC TCCGCTGAGG CAGTAGCATC TGCCCTCCAC 
AAGCTTCATC AAGACCTGTG GAAGACTCAA CAGACCCGGG ATGTTCTGAG GGATCAGGTC 
CAGAAACTGG AAGAGCGTCT AACTGATACT GAGGCTGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGATC TGCAGAGACA GCTCTCCCAG AATCAGGAAG AGAAATCTAA GTGGGAAGGA 
AAGCAGAACT CCCTAGAATC TGAGCTGATG GAACTACATG AAACTATGGC ATCCTTACAG 
AGTCGCCTGC GGAGAGCAGA GCTACAGCGA ATGGAAGCCC AGGGTGAGCG AGAGTTACTT 
CAGGCAGCCA AGGAGAACCT GACAGCCCAG GTGGAACACC TGCAAGCAGC TGTCGTAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGGCATC CTGGAAGAAG ACCTGAGAAC GGCTCGCTCA 
GCACTGAAGC TGAAAAATGA GGAAGTAGAG AGTGAGCGTG AGAGAGCCCA GGCTCTGCAA 
GAGCAGGGCG AACTGAAGGT GGCCCAAGGG AAGGCTCTGC AAGAGAATTT GGCCCTCCTG 
ACCCAGACCC TAGCTGAAAG AGAAGAGGAG GTGGAGACTC TGCGGGGACA AATCCAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GCTTTGGAAT TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC AAGAGGTAGA TCTGCAGCAA GAACAGATTC AGGAGCTAGA GAAGTGTAGG 
TCTGTTTTAG AGCATCTGCC CATGGCCGTC CAGGAGCGAG AGCAGAAGCT GACTGTGCAG 
AGGGAGCAGA TCAGAGAGCT CGAGAAGGAT CGGGAGACTC AGAGGAACGT CTTGGAGCAT 
CAGCTTCTAG AACTTGAGAA GAAAGACCAA ATGATTGAGT CCCAGAGAGG ACAGGTTCAG 
GACCTGAAAA AGCAGTTGGT TACTCTGGAA TGCCTGGCCC TGGAACTGGA GGAAAACCAT 
CACAAGATGG AGTGCCAGCA AAAACTGATC AAGGAGCTGG AGGGCCAGAG GGAAACCCAG 
AGAGTGGCTT TGACCCACCT TACGCTGGAC CTAGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGATCCATGA CCTGGAGAGC CACAGCACCG TTCTGGCAAG AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAA GTCTCAGCGA GAACAGATCG AGGAGCTGCA GAGGCAGAAA 
GAGCATCTGA CTCAGGATCT CGAGAGGAGA GACCAGGAGC TGATGCTGCA GAAGGAGAGG 
ATTCAGGTTC TCGAGGATCA GAGGACCCGG CAGACCAAGA TCCTGGAGGA GGACCTGGAA 
CAGATCAAGC TGTCCTTGAG AGAGCGAGGC CGGGAGCTGA CCACTCAGAG GCAGCTGATG 
CAGGAACGGG CAGAGGAAGG GAAGGGCCCA AGTAAAGCAC AGCGCGGGAG CCTAGAGCAC 
ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTGGAAT GTCAGCAGGA GCATATCCAT 
GAACTCCAGG AGCTCAAAGA CCAGCTGGAG CAGCAGCTCC AGGGCCTGCA CAGGAAGGTA 
GGTGAGACCA GCCTCCTCCT GTCCCAGCGA GAGCAGGAAA TAGTGGTCCT GCAGCAGCAA 
CTGCAGGAAG CCAGGGAACA AGGGGAGCTG AAGGAGCAGT CACTTCAGAG TCAACTGGAT 
GAGGCCCAGA GAGCCCTAGC CCAGAGGGAC CAGGAACTGG AGGCTCTGCA GCAAGAACAG 
CAGCAGGCCC AGGGACAGGA GGAGAGGGTG AAGGAAAAGG CAGACGCCCT CCAGGGAGCT 
CTGGAGCAAG CCCATATGAC ACTGAAGGAG CGTCATGGAG AGCTTCAGGA CCACAAGGAA 
CAGGCACGAA GGCTGGAGGA AGAGCTGGCA GTGGAGGGAC GGCGGGTCCA GGCCCTGGAG 
GAGGTGCTGG GAGACCTAAG GGCTGAGTCT CGGGAACAGG AGAAAGCTCT GTTGGCCCTC 
CAGCAGCAGT GTGCTGAGCA GGCACAGGAG CATGAGGTGG AGACCAGGGC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC AGTGCTCAAG GAACGGGACC AGGAGCTGGA AGCTCTGCGG 
GCAGAAAGTC AGTCCTCCCG GCATCAGGAG GAGGCTGCCC GGGCCCGGGC TGAGGCTCTG 
CAGGAGGCCC TTGGCAAGGC TCATGCTGCC CTGCAGGGGA AAGAGCAGCA TCTCCTCGAG 
CAGGCAGAAT TGAGCCGCAG TCTGGAGGCC AGCACTGCAA CCCTGCAAGC CTCCCTGGAT 
GCCTGCCAGG CACACAGTCG GCAGCTGGAG GAGGCTCTGA GGATACAAGA AGGTGAGATC 
CAGGACCAGG ATCTCCGATA CCAGGAGGAT GTGCAGCAGC TGCAGCAGGC ACTTGCCCAG 
AGGGATGAAG AGCTGAGACA TCAGCAGGAA CGGGAGCAGC TGCTGGAGAA GTCTCTGGCC 
CAGAGGGTCC AAGAGAATAT GATCCAAGAG AAGCAGAATC TGGGGCAAGA GAGAGAAGAG 
GAGGAGATAA GGGGCCTTCA TCAGAGTGTA AGGGAGCTAC AGCTGACTCT AGCCCAAAAG 
GAACAGGAGA TTCTGGAGCT GAGGGAGACC CAGCAAAGGA ACAACCTGGA AGCCTTACCC 
CACAGCCACA AAACCTCCCC AATGGAGGAA CAATCTCTAA AACTTGATTC TTTAGAGCCC 
AGGCTGCAGC GGGAGCTGGA GCGGCTACAG GCAGCCCTGA GACAGACAGA AGCCAGGGAG 
ATTGAGTGGA GGGAGAAGGC CCAGGACTTG GCACTCTCCC TAGCGCAGAC CAAGGCCAGT 
GTCAGCAGTC TGCAGGAGGT AGCCATGTTC CTACAAGCCT CTGTCCTGGA GCGGGACTCA 
GAACAGCAAA GGCTGCAGGA TGAACTGGAG CTCACCAGAC GGGCTCTGGA GAAGGAGCGG 
CTACACAGCC CAGGTGCAAC CAGCACAGCA GAACTGGGGT CCAGAGGGGA GCAGGGTGTG 
CAGCTGGGAG AGGTCTCAGG AGTGGAGGCT GAGCCTAGTC CTGATGGAAT GGAGAAGCAG 
TCATGGAGAC AAAGGCTTGA ACACCTGCAG CAAGCAGTGG CCCGGCTGGA GATTGACAGG 
AGCAGGCTGC AGCGCCACAA TGTCCAGCTG CGGAGTACCT TGGAGCAGGT GGAGCGAGAA 
CGGAGGAAGC TGAAGAGGGA GGCCATGCGT GCGGCCCAGG CAGGGTCCCT AGAGATCAGC 
AAGGCCACGG CTTCTTCACC CACACAGCAG GATGGGAGAG GACAGAAGAA CTCAGATGCC 
AAGTGTGTGG CTGAACTGCA GAAAGAGGTG GTCCTGCTGC AAGCTCAGCT GACTTTGGAG 
CGGAAGCAGA AGCAGGACTA CATCACCCGC TCAGCACAGA CCAGCCGTGA GCTAGCAGGC 
CTGCACCACA GCCTCTCACA CTCACTTCTT GCCGTGGCCC AGGCCCCTGA GGCCACTGTC 
CTGGAGGCAG AGACCCGCAG GCTGGATGAG TCCCTGACTC AAAGTCTGAC ATCCCCAGGG 
CCAGTCCTGC TACACCCCAG CCCCAGCACT ACCCAAGCCG CCTCCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883108.1
CDS	166..6096
Translation	MAPAKGKCGASAAGGLCPGPEGGTAGGAAPGCPGEGASGCWEDESNVLENASIEPGICQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR

Target ORF information:

RefSeq Version	XM_017027619.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 250 (CEP250), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027619.1

ORF Insert Sequence:

ATGGCGCCTG CGAAGGGTAA ATGTGGAGCT TCAGCTGCAG GGGGACTCTG CCCAGGGCCA 
GAAGGAGGAA CAGCAGGAGG AGCTGCACCT GGCTGTCCGG GAGAGGGAGC GTCTGGTTGC 
TGGGAAGATG AGAGCAATGT TTTGGAAAAC GCCAGCATAG AACCTGGCAT ATGTCAGGAG 
ATGCTGATGG GCCTGGAAGC CAAACAGTCA GAATCACTCA GTGAACTGAT CACTCTTCGG 
GAAGCCCTGG AGTCAAGTCA CCTGGAAGGG GAGTTACTGA GGCAAGAGCA AACGGAAGTG 
ACCGCAGCGC TGGCTAGGGC AGAGCAGTCA ATTGCAGAGC TGTCGAGTTC TGAAAACACC 
CTGAAGACAG AAGTAGCTGA TCTTCGGGCT GCAGCTGTCA AGCTCAGTGC CTTAAATGAG 
GCTTTGGCGT TAGATAAAGT TGGGCTGAAC CAGCAGCTTC TCCAGTTAGA GGAGGAGAAC 
CAGTCTGTGT GCAGCAGAAT GGAGGCCGCA GAGCAGGCGA GAAATGCTTT GCAGGTCGAC 
CTGGCGGAGG CAGAGAAGAG GAGGGAAGCC CTGTGGGAAA AGAACACTCA CCTGGAGGCT 
CAGCTGCAGA AAGCTGAGGA GGCTGGGGCT GAGCTGCAGG CAGATCTCAG GGACATCCAA 
GAAGAGAAGG AAGAAATTCA AAAGAAACTA AGTGAGTCAC GTCACCAGCA GGAGGCAGCC 
ACGACTCAGC TGGAGCAGCT ACATCAGGAG GCAAAGCGAC AGGAAGAAGT GCTTGCCAGG 
GCAGTCCAGG AGAAGGAGGC CCTAGTACGA GAGAAAGCGG CTCTAGAGGT GCGGCTGCAG 
GCCGTGGAGC GTGACCGGCA GGACCTCGCT GAACAACTAC AGGGGCTCAG CTCAGCCAAG 
GAGCTACTGG AGAGCAGTCT GTTTGAAGCC CAACAACAAA ATTCTGTGAT AGAGGTCACC 
AAGGGGCAGC TGGAGGTCCA GATTCAAACT GTCACTCAAG CCAAGGAAGT AATCCAAGGG 
GAAGTGAGGT GCCTGAAGCT GGAACTGGAC ACTGAACGGA GTCAGGCAGA GCAGGAGCGG 
GATGCTGCAG CCAGACAGCT GGCCCAGGCT GAGCAAGAAG GGAAGACTGC CTTGGAGCAG 
CAGAAGGCAG CCCATGAGAA AGAGGTGAAC CAGCTCCGGG AGAAATGGGA GAAGGAGCGC 
TCCTGGCACC AGCAGGAGCT GGCAAAGGCT CTGGAGAGCT TAGAAAGGGA AAAAATGGAG 
CTGGAAATGA GGCTAAAGGA GCAGCAGACA GAAATGGAGG CCATCCAGGC CCAGAGGGAA 
GAAGAACGGA CCCAGGCAGA GAGTGCCCTA TGCCAGATGC AGCTGGAAAC AGAGAAGGAG 
AGAGTATCCC TCCTGGAGAC ACTGCTGCAG ACGCAGAAGG AGCTAGCAGA TGCCAGCCAA 
CAACTGGAAC GACTGAGGCA GGACATGAAA GTCCAGAAAT TAAAGGAGCA GGAGACCACT 
GGGATACTAC AGACCCAGCT CCAGGAGGCT CAACGGGAGC TGAAGGAGGC AGCCCGGCAG 
CACAGAGATG ACCTTGCTGC CCTCCAAGAA GAGAGCAGCT CCCTGCTGCA GGATAAGATG 
GACCTGCAGA AGCAGGTGGA GGACTTGAAG TCTCAGCTGG TGGCCCAGGA TGACTCCCAG 
AGGCTGGTGG AGCAGGAGGT TCAGGAGAAG CTGAGAGAGA CCCAGGAGTA TAACCGAATT 
CAGAAGGAGC TGGAGAGAGA GAAAGCCAGC CTGACTCTCT CACTGATGGA AAAGGAACAG 
AGACTCCTTG TTTTACAAGA AGCTGACTCT ATTCGACAAC AAGAGCTGAG TGCCCTGCGC 
CAGGACATGC AGGAGGCCCA GGGAGAACAG AAAGAGCTCA GTGCTCAGAT GGAATTACTA 
AGGCAAGAGG TGAAGGAAAA GGAGGCTGAC TTTCTGGCCC AGGAAGCACA GCTGCTGGAG 
GAGCTGGAGG CGTCTCATAT CACGGAGCAG CAGCTGCGAG CCTCCTTGTG GGCCCAGGAA 
GCCAAGGCAG CCCAACTACA GCTGCGACTG CGCAGCACAG AGAGCCAGCT AGAAGCGCTG 
GCCGCAGAGC AGCAGCCCGG GAACCAGGCC CAGGCCCAGG CCCAGCTGGC CAGCCTCTAC 
TCTGCCCTGC AGCAGGCCCT GGGGTCTGTT TGTGAGAGCA GGCCTGAGCT GAGTGGTGGG 
GGAGACTCTG CTCCTTCCGT CTGGGGCCTT GAGCCAGACC AGAATGGAGC TAGGAGCCTC 
TTTAAGAGAG GGCCCCTGCT GACTGCTCTC TCCGCTGAGG CAGTAGCATC TGCCCTCCAC 
AAGCTTCATC AAGACCTGTG GAAGACTCAA CAGACCCGGG ATGTTCTGAG GGATCAGGTC 
CAGAAACTGG AAGAGCGTCT AACTGATACT GAGGCTGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGATC TGCAGAGACA GCTCTCCCAG AATCAGGAAG AGAAATCTAA GTGGGAAGGA 
AAGCAGAACT CCCTAGAATC TGAGCTGATG GAACTACATG AAACTATGGC ATCCTTACAG 
AGTCGCCTGC GGAGAGCAGA GCTACAGCGA ATGGAAGCCC AGGGTGAGCG AGAGTTACTT 
CAGGCAGCCA AGGAGAACCT GACAGCCCAG GTGGAACACC TGCAAGCAGC TGTCGTAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGGCATC CTGGAAGAAG ACCTGAGAAC GGCTCGCTCA 
GCACTGAAGC TGAAAAATGA GGAAGTAGAG AGTGAGCGTG AGAGAGCCCA GGCTCTGCAA 
GAGCAGGGCG AACTGAAGGT GGCCCAAGGG AAGGCTCTGC AAGAGAATTT GGCCCTCCTG 
ACCCAGACCC TAGCTGAAAG AGAAGAGGAG GTGGAGACTC TGCGGGGACA AATCCAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GCTTTGGAAT TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC AAGAGGTAGA TCTGCAGCAA GAACAGATTC AGGAGCTAGA GAAGTGTAGG 
TCTGTTTTAG AGCATCTGCC CATGGCCGTC CAGGAGCGAG AGCAGAAGCT GACTGTGCAG 
AGGGAGCAGA TCAGAGAGCT CGAGAAGGAT CGGGAGACTC AGAGGAACGT CTTGGAGCAT 
CAGCTTCTAG AACTTGAGAA GAAAGACCAA ATGATTGAGT CCCAGAGAGG ACAGGTTCAG 
GACCTGAAAA AGCAGTTGGT TACTCTGGAA TGCCTGGCCC TGGAACTGGA GGAAAACCAT 
CACAAGATGG AGTGCCAGCA AAAACTGATC AAGGAGCTGG AGGGCCAGAG GGAAACCCAG 
AGAGTGGCTT TGACCCACCT TACGCTGGAC CTAGAAGAAA GGAGCCAGGA GCTGCAGGCA 
CAAAGCAGCC AGATCCATGA CCTGGAGAGC CACAGCACCG TTCTGGCAAG AGAGCTGCAG 
GAGAGGGACC AGGAGGTGAA GTCTCAGCGA GAACAGATCG AGGAGCTGCA GAGGCAGAAA 
GAGCATCTGA CTCAGGATCT CGAGAGGAGA GACCAGGAGC TGATGCTGCA GAAGGAGAGG 
ATTCAGGTTC TCGAGGATCA GAGGACCCGG CAGACCAAGA TCCTGGAGGA GGACCTGGAA 
CAGATCAAGC TGTCCTTGAG AGAGCGAGGC CGGGAGCTGA CCACTCAGAG GCAGCTGATG 
CAGGAACGGG CAGAGGAAGG GAAGGGCCCA AGTAAAGCAC AGCGCGGGAG CCTAGAGCAC 
ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTGGAAT GTCAGCAGGA GCATATCCAT 
GAACTCCAGG AGCTCAAAGA CCAGCTGGAG CAGCAGCTCC AGGGCCTGCA CAGGAAGGTA 
GGTGAGACCA GCCTCCTCCT GTCCCAGCGA GAGCAGGAAA TAGTGGTCCT GCAGCAGCAA 
CTGCAGGAAG CCAGGGAACA AGGGGAGCTG AAGGAGCAGT CACTTCAGAG TCAACTGGAT 
GAGGCCCAGA GAGCCCTAGC CCAGAGGGAC CAGGAACTGG AGGCTCTGCA GCAAGAACAG 
CAGCAGGCCC AGGGACAGGA GGAGAGGGTG AAGGAAAAGG CAGACGCCCT CCAGGGAGCT 
CTGGAGCAAG CCCATATGAC ACTGAAGGAG CGTCATGGAG AGCTTCAGGA CCACAAGGAA 
CAGGCACGAA GGCTGGAGGA AGAGCTGGCA GTGGAGGGAC GGCGGGTCCA GGCCCTGGAG 
GAGGTGCTGG GAGACCTAAG GGCTGAGTCT CGGGAACAGG AGAAAGCTCT GTTGGCCCTC 
CAGCAGCAGT GTGCTGAGCA GGCACAGGAG CATGAGGTGG AGACCAGGGC CCTGCAGGAC 
AGCTGGCTGC AGGCCCAGGC AGTGCTCAAG GAACGGGACC AGGAGCTGGA AGCTCTGCGG 
GCAGAAAGTC AGTCCTCCCG GCATCAGGAG GAGGCTGCCC GGGCCCGGGC TGAGGCTCTG 
CAGGAGGCCC TTGGCAAGGC TCATGCTGCC CTGCAGGGGA AAGAGCAGCA TCTCCTCGAG 
CAGGCAGAAT TGAGCCGCAG TCTGGAGGCC AGCACTGCAA CCCTGCAAGC CTCCCTGGAT 
GCCTGCCAGG CACACAGTCG GCAGCTGGAG GAGGCTCTGA GGATACAAGA AGGTGAGATC 
CAGGACCAGG ATCTCCGATA CCAGGAGGAT GTGCAGCAGC TGCAGCAGGC ACTTGCCCAG 
AGGGATGAAG AGCTGAGACA TCAGCAGGAA CGGGAGCAGC TGCTGGAGAA GTCTCTGGCC 
CAGAGGGTCC AAGAGAATAT GATCCAAGAG AAGCAGAATC TGGGGCAAGA GAGAGAAGAG 
GAGGAGATAA GGGGCCTTCA TCAGAGTGTA AGGGAGCTAC AGCTGACTCT AGCCCAAAAG 
GAACAGGAGA TTCTGGAGCT GAGGGAGACC CAGCAAAGGA ACAACCTGGA AGCCTTACCC 
CACAGCCACA AAACCTCCCC AATGGAGGAA CAATCTCTAA AACTTGATTC TTTAGAGCCC 
AGGCTGCAGC GGGAGCTGGA GCGGCTACAG GCAGCCCTGA GACAGACAGA AGCCAGGGAG 
ATTGAGTGGA GGGAGAAGGC CCAGGACTTG GCACTCTCCC TAGCGCAGAC CAAGGCCAGT 
GTCAGCAGTC TGCAGGAGGT AGCCATGTTC CTACAAGCCT CTGTCCTGGA GCGGGACTCA 
GAACAGCAAA GGCTGCAGGA TGAACTGGAG CTCACCAGAC GGGCTCTGGA GAAGGAGCGG 
CTACACAGCC CAGGTGCAAC CAGCACAGCA GAACTGGGGT CCAGAGGGGA GCAGGGTGTG 
CAGCTGGGAG AGGTCTCAGG AGTGGAGGCT GAGCCTAGTC CTGATGGAAT GGAGAAGCAG 
TCATGGAGAC AAAGGCTTGA ACACCTGCAG CAAGCAGTGG CCCGGCTGGA GATTGACAGG 
AGCAGGCTGC AGCGCCACAA TGTCCAGCTG CGGAGTACCT TGGAGCAGGT GGAGCGAGAA 
CGGAGGAAGC TGAAGAGGGA GGCCATGCGT GCGGCCCAGG CAGGGTCCCT AGAGATCAGC 
AAGGCCACGG CTTCTTCACC CACACAGCAG GATGGGAGAG GACAGAAGAA CTCAGATGCC 
AAGTGTGTGG CTGAACTGCA GAAAGAGGTG GTCCTGCTGC AAGCTCAGCT GACTTTGGAG 
CGGAAGCAGA AGCAGGACTA CATCACCCGC TCAGCACAGA CCAGCCGTGA GCTAGCAGGC 
CTGCACCACA GCCTCTCACA CTCACTTCTT GCCGTGGCCC AGGCCCCTGA GGCCACTGTC 
CTGGAGGCAG AGACCCGCAG GCTGGATGAG TCCCTGACTC AAAGTCTGAC ATCCCCAGGG 
CCAGTCCTGC TACACCCCAG CCCCAGCACT ACCCAAGCCG CCTCCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CEP250
Entrez Gene ID	11190
Full Name	centrosomal protein 250
Synonyms	C-NAP1,CEP2,CNAP1
General protein information	Preferred Names centrosomal protein 250 Names centrosome-associated protein CEP250 250 kDa centrosomal protein centrosomal Nek2-associated protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015].
Disorder MIM:	609689

Gallus gallus (chicken)	LOC100858952	XP_003642497.2
Homo sapiens (human)	CEP250	NP_009117.2
Pan troglodytes (chimpanzee)	CEP250	XP_003316929.1
Mus musculus (house mouse)	Cep250	NP_001123471.1
Bos taurus (cattle)	CEP250	XP_005215062.1
Gallus gallus (chicken)	CEP250	XP_417323.4
	CEP250	XP_001099508.1
Canis lupus familiaris (dog)	CEP250	XP_542975.3
Rattus norvegicus (Norway rat)	Cep250	XP_006235436.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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CEP250 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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