ADGRF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following ADGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe15376 XM_014118496.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_005627446.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118497.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118495.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118498.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_022426131.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118499.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118500.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15376 XM_014118501.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15377 XM_014118503.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe15377 XM_014118504.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe292497 XM_022426128.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe292498 XM_022426130.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe292499 XM_022426133.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39603 XM_014118502.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39603 XM_022426132.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_022426131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281839.1
    CDS938..3733
    Translation

    Target ORF information:

    RefSeq Version XM_022426131.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_005627446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005627503.1
    CDS3141..5936
    Translation

    Target ORF information:

    RefSeq Version XM_005627446.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005627446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973976.1
    CDS2972..5767
    Translation

    Target ORF information:

    RefSeq Version XM_014118501.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973973.1
    CDS986..3781
    Translation

    Target ORF information:

    RefSeq Version XM_014118498.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118499.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973974.1
    CDS3138..5933
    Translation

    Target ORF information:

    RefSeq Version XM_014118499.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118497.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973972.1
    CDS3180..5975
    Translation

    Target ORF information:

    RefSeq Version XM_014118497.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973970.1
    CDS3426..6221
    Translation

    Target ORF information:

    RefSeq Version XM_014118495.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973971.1
    CDS1012..3807
    Translation

    Target ORF information:

    RefSeq Version XM_014118496.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15376
    Clone ID Related Accession (Same CDS sequence) XM_022426131.1 , XM_005627446.2 , XM_014118501.1 , XM_014118498.1 , XM_014118499.1 , XM_014118497.1 , XM_014118495.1 , XM_014118496.1 , XM_014118500.1
    Accession Version XM_014118500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973975.1
    CDS3057..5852
    Translation

    Target ORF information:

    RefSeq Version XM_014118500.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGCATTTAT GTACTTTCTC ATACTGGTGA ATCCTCAAAT
    GACATCATGC TAACTCAGTT TTTATCAACT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15377
    Clone ID Related Accession (Same CDS sequence) XM_014118504.1 , XM_014118503.1
    Accession Version XM_014118504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAAGTCC GCGTGCTCTG GCTCATCTCT TTCTTCACAC TCATTGAGGG TTGGGAAGGC 
    TTCCCGGGGA GAACTGAAGG CATCAAAACA AAAAGAGGAC TCATTTTGAA TAAGAAAAAG
    CATCCAGGCC CAGTTCGGGA GTATGAGCTA CTGCTTCAGG TGTCCTATAG AGATTCCAAG
    GAGAAAAGAG ACCTGAAGAA TTTTCTGAAG CTTTTGAAGC CTCCATCATT ATGGTTACAT
    GCTCCAGTGA AGATTATTAG AGCAAAAGCC ACTACACACT GTGGCAACCA GAATGGGAGT
    TTGCAGTGTG CCTGTGAGGA CGGCTACACC TGGTTTCCTC CCTCATGCCT TGATCCCCAG
    AAATGCTACC TTCACACAGC TGGAGCACTC CAGAGCTGTC ACTGTCATGT CAACAACCTC
    AGTCAGAGCA CCAATTTCTG TGAGAGAACA AAGGTTTGGG GCACTTTCAA AATTAATGAA
    AGATTTACCA AAGACCTTTT GAATTCATCT TCTCCTTCAT ACTCCAAATA TGCGACTGGA
    ATTGAAATTC AACTTAAGGA GGCATTCAAA AGAATTCAAG ATTTTGAGTC AGTTCGTGTC
    ACCCAATTTC GTCCTTACAG AGATGGAAGA ATCTTTGCTG GGTTTGAAGT TGTGAGTTCC
    AGCAGCACAT CTGAGCTGCT GTCAGCCATT AAACAGGTGG CTAAGAAGGT GAAGGAAGGC
    CTTCACAAGC TGTTCCCACT AGAAGACAGC TCCTTCAGAG TATTTGCAAA AGCCCAGTGT
    AACAGCATTG TCTTTGGATT TGGGTCCAGG AATGATGAGT TTACTCTTCC CTGTAGCAGT
    GGCTATACTG GAAACATTAC GGTCAGATGC CAGCCCTCTG GGTGGCAGGT CCTCAGGGAG
    ATGTGTGTGC ACTCTCAGCT GGAAGAATTG AAGAAGAATC TCAGTGTGAT GGCAGGCCAT
    GCCACCGAGA CAGCTGTGTC ATCCTTGGTG CGAAATCTTT CCGTCGTCAT TCGGCAAAAC
    CCATCAACCA CAGCTGGCAA TTTGGCTTCA GTAGTGTCAA TTCTGGACAG CGTCTTGTCC
    CTATCACTGG CAAGCCATTT CAAGGTGTCC AATTCAACAA TGGAGGATGT CATCAGTATA
    GCTGACCACA TCCTCAGTTC AGCATCAGTA ACCAACTGGA CCGTGTTACT GCAGGAAGAA
    CAGCAAGCCA GCTCGAGGTT TCTGAAGACA CTAGAAAACA TCAGTAGCCT CGTACCTCCA
    ACAGCTCTGC CTCTGAATTT TTCTCGGGAA TTTATTGACT GGAAAGGGAT TCCGGTGTCT
    GCAAGCCAAC TTAAGATGGG TTACAACTAT CAGACGGAAC TGTTCCCCAC AAATGCCTCC
    ATTCCTGTCA AAGGCCATGT GTTGATTGGG TCAGACCAAT TCCGGTGGTC TCTTCCAGAA
    ACAATTATCA GCATGGCCTC GTTGACCCTG GGGAATATTC TATCTACTAC TCAAAATGGA
    AATGCTCAGG TCAATGGGCC CATGATATCC ACGGTTATCC AAAATTATTC CATAAATGAG
    ATTTTCCTGA CTTTTTCCAA GATAGAGTCA AATCTGAGCC AACCTCATTG TGTGTTTTGG
    GATTTCAGTC GTTTGCAGTG GAACAATGCG GGCTGCCACC TAGTGAACGA AACAGCAGAC
    ACGGTGATGT GCCGATGTAC TCACCTGACC TCCTTCTCCA TACTGATGTC ACCCTTTGTC
    CCTCCTGCCA TCATCCCCGT GGTGAAATGG ATCACCTTTG TGGGACTGGG CATCTCCATT
    GGAAGTCTCA CCTTATGCCT AATCATTGAA GCTCTATTTT GGAAGCAGGT CAAGAAAAAC
    CAAACCTCCT ACACACGTCA CGTTTGCATG GTGAATGTAG CCCTGTGCCT CCTGATTGCT
    GATGTTTGGT TTATTGTTGC TGCCACTGAA GGTTCCACCG TAAACCTTTC TGGAGTCTGC
    ATAGCTGCAG TGTTCTTTAT GCACTTTTTC TACCTCTCTT TGTTCTTTTG GATGCTCATG
    CTTGGCATCC TCCTGGCTTA TCGGATCCTC CTTGTGTTCC ATCACATGGC CATGCCTTTG
    ATGATGGCTG TCGGGTTCTG CCTGGGGTAT GGGTGCCCTC TCATTATATC TGTCATCACG
    ATTGCAGTCA CCCAGCCTAG CAATGCCTAC AAAAGGAAAG ATGCGTGTTG GCTTAACTGG
    TCTGATAGGA GCAAACCCTT ACTGGCCTTT GCTGTTCCCG CACTGACTAT TGTGGCTGTG
    AATTTGGCTG TGGTGCTAGT AGTTCTCACG AAGCTCTGGA GGCCCACTGT TGGAGAAAGA
    CCGGGTCAGG AAAACAAGGC CACTATTGTC CGCATGGGGA AGAGCCTCCT CATCCTGACC
    CCTCTGCTGG GGCTCACCTG GGGCTTTGGC ATAGGAACAA TAGTGGACAA CCAGAATCTG
    GCTTGGCATG TTATTTTTGC ATTACTCAAT GCATTCCAGG GTTTTTTCAT CTTATGTTTT
    GGAATGCTCT TGGACAGTAA GCTGCGACAA TTGCTGTTCA ACAAATTATC TCCTTTCAGC
    TCTTGGAAGC AAGCATCAAA GCAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA
    AAGCCTTTCA ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC
    TCAAATGACA TCATGCTAAC TCAGTTTTTA TCAACTGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973979.1
    CDS2947..5688
    Translation

    Target ORF information:

    RefSeq Version XM_014118504.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAAGTCC GCGTGCTCTG GCTCATCTCT TTCTTCACAC TCATTGAGGG TTGGGAAGGC 
    TTCCCGGGGA GAACTGAAGG CATCAAAACA AAAAGAGGAC TCATTTTGAA TAAGAAAAAG
    CATCCAGGCC CAGTTCGGGA GTATGAGCTA CTGCTTCAGG TGTCCTATAG AGATTCCAAG
    GAGAAAAGAG ACCTGAAGAA TTTTCTGAAG CTTTTGAAGC CTCCATCATT ATGGTTACAT
    GCTCCAGTGA AGATTATTAG AGCAAAAGCC ACTACACACT GTGGCAACCA GAATGGGAGT
    TTGCAGTGTG CCTGTGAGGA CGGCTACACC TGGTTTCCTC CCTCATGCCT TGATCCCCAG
    AAATGCTACC TTCACACAGC TGGAGCACTC CAGAGCTGTC ACTGTCATGT CAACAACCTC
    AGTCAGAGCA CCAATTTCTG TGAGAGAACA AAGGTTTGGG GCACTTTCAA AATTAATGAA
    AGATTTACCA AAGACCTTTT GAATTCATCT TCTCCTTCAT ACTCCAAATA TGCGACTGGA
    ATTGAAATTC AACTTAAGGA GGCATTCAAA AGAATTCAAG ATTTTGAGTC AGTTCGTGTC
    ACCCAATTTC GTCCTTACAG AGATGGAAGA ATCTTTGCTG GGTTTGAAGT TGTGAGTTCC
    AGCAGCACAT CTGAGCTGCT GTCAGCCATT AAACAGGTGG CTAAGAAGGT GAAGGAAGGC
    CTTCACAAGC TGTTCCCACT AGAAGACAGC TCCTTCAGAG TATTTGCAAA AGCCCAGTGT
    AACAGCATTG TCTTTGGATT TGGGTCCAGG AATGATGAGT TTACTCTTCC CTGTAGCAGT
    GGCTATACTG GAAACATTAC GGTCAGATGC CAGCCCTCTG GGTGGCAGGT CCTCAGGGAG
    ATGTGTGTGC ACTCTCAGCT GGAAGAATTG AAGAAGAATC TCAGTGTGAT GGCAGGCCAT
    GCCACCGAGA CAGCTGTGTC ATCCTTGGTG CGAAATCTTT CCGTCGTCAT TCGGCAAAAC
    CCATCAACCA CAGCTGGCAA TTTGGCTTCA GTAGTGTCAA TTCTGGACAG CGTCTTGTCC
    CTATCACTGG CAAGCCATTT CAAGGTGTCC AATTCAACAA TGGAGGATGT CATCAGTATA
    GCTGACCACA TCCTCAGTTC AGCATCAGTA ACCAACTGGA CCGTGTTACT GCAGGAAGAA
    CAGCAAGCCA GCTCGAGGTT TCTGAAGACA CTAGAAAACA TCAGTAGCCT CGTACCTCCA
    ACAGCTCTGC CTCTGAATTT TTCTCGGGAA TTTATTGACT GGAAAGGGAT TCCGGTGTCT
    GCAAGCCAAC TTAAGATGGG TTACAACTAT CAGACGGAAC TGTTCCCCAC AAATGCCTCC
    ATTCCTGTCA AAGGCCATGT GTTGATTGGG TCAGACCAAT TCCGGTGGTC TCTTCCAGAA
    ACAATTATCA GCATGGCCTC GTTGACCCTG GGGAATATTC TATCTACTAC TCAAAATGGA
    AATGCTCAGG TCAATGGGCC CATGATATCC ACGGTTATCC AAAATTATTC CATAAATGAG
    ATTTTCCTGA CTTTTTCCAA GATAGAGTCA AATCTGAGCC AACCTCATTG TGTGTTTTGG
    GATTTCAGTC GTTTGCAGTG GAACAATGCG GGCTGCCACC TAGTGAACGA AACAGCAGAC
    ACGGTGATGT GCCGATGTAC TCACCTGACC TCCTTCTCCA TACTGATGTC ACCCTTTGTC
    CCTCCTGCCA TCATCCCCGT GGTGAAATGG ATCACCTTTG TGGGACTGGG CATCTCCATT
    GGAAGTCTCA CCTTATGCCT AATCATTGAA GCTCTATTTT GGAAGCAGGT CAAGAAAAAC
    CAAACCTCCT ACACACGTCA CGTTTGCATG GTGAATGTAG CCCTGTGCCT CCTGATTGCT
    GATGTTTGGT TTATTGTTGC TGCCACTGAA GGTTCCACCG TAAACCTTTC TGGAGTCTGC
    ATAGCTGCAG TGTTCTTTAT GCACTTTTTC TACCTCTCTT TGTTCTTTTG GATGCTCATG
    CTTGGCATCC TCCTGGCTTA TCGGATCCTC CTTGTGTTCC ATCACATGGC CATGCCTTTG
    ATGATGGCTG TCGGGTTCTG CCTGGGGTAT GGGTGCCCTC TCATTATATC TGTCATCACG
    ATTGCAGTCA CCCAGCCTAG CAATGCCTAC AAAAGGAAAG ATGCGTGTTG GCTTAACTGG
    TCTGATAGGA GCAAACCCTT ACTGGCCTTT GCTGTTCCCG CACTGACTAT TGTGGCTGTG
    AATTTGGCTG TGGTGCTAGT AGTTCTCACG AAGCTCTGGA GGCCCACTGT TGGAGAAAGA
    CCGGGTCAGG AAAACAAGGC CACTATTGTC CGCATGGGGA AGAGCCTCCT CATCCTGACC
    CCTCTGCTGG GGCTCACCTG GGGCTTTGGC ATAGGAACAA TAGTGGACAA CCAGAATCTG
    GCTTGGCATG TTATTTTTGC ATTACTCAAT GCATTCCAGG GTTTTTTCAT CTTATGTTTT
    GGAATGCTCT TGGACAGTAA GCTGCGACAA TTGCTGTTCA ACAAATTATC TCCTTTCAGC
    TCTTGGAAGC AAGCATCAAA GCAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA
    AAGCCTTTCA ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC
    TCAAATGACA TCATGCTAAC TCAGTTTTTA TCAACTGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe15377
    Clone ID Related Accession (Same CDS sequence) XM_014118504.1 , XM_014118503.1
    Accession Version XM_014118503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAAGTCC GCGTGCTCTG GCTCATCTCT TTCTTCACAC TCATTGAGGG TTGGGAAGGC 
    TTCCCGGGGA GAACTGAAGG CATCAAAACA AAAAGAGGAC TCATTTTGAA TAAGAAAAAG
    CATCCAGGCC CAGTTCGGGA GTATGAGCTA CTGCTTCAGG TGTCCTATAG AGATTCCAAG
    GAGAAAAGAG ACCTGAAGAA TTTTCTGAAG CTTTTGAAGC CTCCATCATT ATGGTTACAT
    GCTCCAGTGA AGATTATTAG AGCAAAAGCC ACTACACACT GTGGCAACCA GAATGGGAGT
    TTGCAGTGTG CCTGTGAGGA CGGCTACACC TGGTTTCCTC CCTCATGCCT TGATCCCCAG
    AAATGCTACC TTCACACAGC TGGAGCACTC CAGAGCTGTC ACTGTCATGT CAACAACCTC
    AGTCAGAGCA CCAATTTCTG TGAGAGAACA AAGGTTTGGG GCACTTTCAA AATTAATGAA
    AGATTTACCA AAGACCTTTT GAATTCATCT TCTCCTTCAT ACTCCAAATA TGCGACTGGA
    ATTGAAATTC AACTTAAGGA GGCATTCAAA AGAATTCAAG ATTTTGAGTC AGTTCGTGTC
    ACCCAATTTC GTCCTTACAG AGATGGAAGA ATCTTTGCTG GGTTTGAAGT TGTGAGTTCC
    AGCAGCACAT CTGAGCTGCT GTCAGCCATT AAACAGGTGG CTAAGAAGGT GAAGGAAGGC
    CTTCACAAGC TGTTCCCACT AGAAGACAGC TCCTTCAGAG TATTTGCAAA AGCCCAGTGT
    AACAGCATTG TCTTTGGATT TGGGTCCAGG AATGATGAGT TTACTCTTCC CTGTAGCAGT
    GGCTATACTG GAAACATTAC GGTCAGATGC CAGCCCTCTG GGTGGCAGGT CCTCAGGGAG
    ATGTGTGTGC ACTCTCAGCT GGAAGAATTG AAGAAGAATC TCAGTGTGAT GGCAGGCCAT
    GCCACCGAGA CAGCTGTGTC ATCCTTGGTG CGAAATCTTT CCGTCGTCAT TCGGCAAAAC
    CCATCAACCA CAGCTGGCAA TTTGGCTTCA GTAGTGTCAA TTCTGGACAG CGTCTTGTCC
    CTATCACTGG CAAGCCATTT CAAGGTGTCC AATTCAACAA TGGAGGATGT CATCAGTATA
    GCTGACCACA TCCTCAGTTC AGCATCAGTA ACCAACTGGA CCGTGTTACT GCAGGAAGAA
    CAGCAAGCCA GCTCGAGGTT TCTGAAGACA CTAGAAAACA TCAGTAGCCT CGTACCTCCA
    ACAGCTCTGC CTCTGAATTT TTCTCGGGAA TTTATTGACT GGAAAGGGAT TCCGGTGTCT
    GCAAGCCAAC TTAAGATGGG TTACAACTAT CAGACGGAAC TGTTCCCCAC AAATGCCTCC
    ATTCCTGTCA AAGGCCATGT GTTGATTGGG TCAGACCAAT TCCGGTGGTC TCTTCCAGAA
    ACAATTATCA GCATGGCCTC GTTGACCCTG GGGAATATTC TATCTACTAC TCAAAATGGA
    AATGCTCAGG TCAATGGGCC CATGATATCC ACGGTTATCC AAAATTATTC CATAAATGAG
    ATTTTCCTGA CTTTTTCCAA GATAGAGTCA AATCTGAGCC AACCTCATTG TGTGTTTTGG
    GATTTCAGTC GTTTGCAGTG GAACAATGCG GGCTGCCACC TAGTGAACGA AACAGCAGAC
    ACGGTGATGT GCCGATGTAC TCACCTGACC TCCTTCTCCA TACTGATGTC ACCCTTTGTC
    CCTCCTGCCA TCATCCCCGT GGTGAAATGG ATCACCTTTG TGGGACTGGG CATCTCCATT
    GGAAGTCTCA CCTTATGCCT AATCATTGAA GCTCTATTTT GGAAGCAGGT CAAGAAAAAC
    CAAACCTCCT ACACACGTCA CGTTTGCATG GTGAATGTAG CCCTGTGCCT CCTGATTGCT
    GATGTTTGGT TTATTGTTGC TGCCACTGAA GGTTCCACCG TAAACCTTTC TGGAGTCTGC
    ATAGCTGCAG TGTTCTTTAT GCACTTTTTC TACCTCTCTT TGTTCTTTTG GATGCTCATG
    CTTGGCATCC TCCTGGCTTA TCGGATCCTC CTTGTGTTCC ATCACATGGC CATGCCTTTG
    ATGATGGCTG TCGGGTTCTG CCTGGGGTAT GGGTGCCCTC TCATTATATC TGTCATCACG
    ATTGCAGTCA CCCAGCCTAG CAATGCCTAC AAAAGGAAAG ATGCGTGTTG GCTTAACTGG
    TCTGATAGGA GCAAACCCTT ACTGGCCTTT GCTGTTCCCG CACTGACTAT TGTGGCTGTG
    AATTTGGCTG TGGTGCTAGT AGTTCTCACG AAGCTCTGGA GGCCCACTGT TGGAGAAAGA
    CCGGGTCAGG AAAACAAGGC CACTATTGTC CGCATGGGGA AGAGCCTCCT CATCCTGACC
    CCTCTGCTGG GGCTCACCTG GGGCTTTGGC ATAGGAACAA TAGTGGACAA CCAGAATCTG
    GCTTGGCATG TTATTTTTGC ATTACTCAAT GCATTCCAGG GTTTTTTCAT CTTATGTTTT
    GGAATGCTCT TGGACAGTAA GCTGCGACAA TTGCTGTTCA ACAAATTATC TCCTTTCAGC
    TCTTGGAAGC AAGCATCAAA GCAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA
    AAGCCTTTCA ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC
    TCAAATGACA TCATGCTAAC TCAGTTTTTA TCAACTGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973978.1
    CDS3243..5984
    Translation

    Target ORF information:

    RefSeq Version XM_014118503.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAAGTCC GCGTGCTCTG GCTCATCTCT TTCTTCACAC TCATTGAGGG TTGGGAAGGC 
    TTCCCGGGGA GAACTGAAGG CATCAAAACA AAAAGAGGAC TCATTTTGAA TAAGAAAAAG
    CATCCAGGCC CAGTTCGGGA GTATGAGCTA CTGCTTCAGG TGTCCTATAG AGATTCCAAG
    GAGAAAAGAG ACCTGAAGAA TTTTCTGAAG CTTTTGAAGC CTCCATCATT ATGGTTACAT
    GCTCCAGTGA AGATTATTAG AGCAAAAGCC ACTACACACT GTGGCAACCA GAATGGGAGT
    TTGCAGTGTG CCTGTGAGGA CGGCTACACC TGGTTTCCTC CCTCATGCCT TGATCCCCAG
    AAATGCTACC TTCACACAGC TGGAGCACTC CAGAGCTGTC ACTGTCATGT CAACAACCTC
    AGTCAGAGCA CCAATTTCTG TGAGAGAACA AAGGTTTGGG GCACTTTCAA AATTAATGAA
    AGATTTACCA AAGACCTTTT GAATTCATCT TCTCCTTCAT ACTCCAAATA TGCGACTGGA
    ATTGAAATTC AACTTAAGGA GGCATTCAAA AGAATTCAAG ATTTTGAGTC AGTTCGTGTC
    ACCCAATTTC GTCCTTACAG AGATGGAAGA ATCTTTGCTG GGTTTGAAGT TGTGAGTTCC
    AGCAGCACAT CTGAGCTGCT GTCAGCCATT AAACAGGTGG CTAAGAAGGT GAAGGAAGGC
    CTTCACAAGC TGTTCCCACT AGAAGACAGC TCCTTCAGAG TATTTGCAAA AGCCCAGTGT
    AACAGCATTG TCTTTGGATT TGGGTCCAGG AATGATGAGT TTACTCTTCC CTGTAGCAGT
    GGCTATACTG GAAACATTAC GGTCAGATGC CAGCCCTCTG GGTGGCAGGT CCTCAGGGAG
    ATGTGTGTGC ACTCTCAGCT GGAAGAATTG AAGAAGAATC TCAGTGTGAT GGCAGGCCAT
    GCCACCGAGA CAGCTGTGTC ATCCTTGGTG CGAAATCTTT CCGTCGTCAT TCGGCAAAAC
    CCATCAACCA CAGCTGGCAA TTTGGCTTCA GTAGTGTCAA TTCTGGACAG CGTCTTGTCC
    CTATCACTGG CAAGCCATTT CAAGGTGTCC AATTCAACAA TGGAGGATGT CATCAGTATA
    GCTGACCACA TCCTCAGTTC AGCATCAGTA ACCAACTGGA CCGTGTTACT GCAGGAAGAA
    CAGCAAGCCA GCTCGAGGTT TCTGAAGACA CTAGAAAACA TCAGTAGCCT CGTACCTCCA
    ACAGCTCTGC CTCTGAATTT TTCTCGGGAA TTTATTGACT GGAAAGGGAT TCCGGTGTCT
    GCAAGCCAAC TTAAGATGGG TTACAACTAT CAGACGGAAC TGTTCCCCAC AAATGCCTCC
    ATTCCTGTCA AAGGCCATGT GTTGATTGGG TCAGACCAAT TCCGGTGGTC TCTTCCAGAA
    ACAATTATCA GCATGGCCTC GTTGACCCTG GGGAATATTC TATCTACTAC TCAAAATGGA
    AATGCTCAGG TCAATGGGCC CATGATATCC ACGGTTATCC AAAATTATTC CATAAATGAG
    ATTTTCCTGA CTTTTTCCAA GATAGAGTCA AATCTGAGCC AACCTCATTG TGTGTTTTGG
    GATTTCAGTC GTTTGCAGTG GAACAATGCG GGCTGCCACC TAGTGAACGA AACAGCAGAC
    ACGGTGATGT GCCGATGTAC TCACCTGACC TCCTTCTCCA TACTGATGTC ACCCTTTGTC
    CCTCCTGCCA TCATCCCCGT GGTGAAATGG ATCACCTTTG TGGGACTGGG CATCTCCATT
    GGAAGTCTCA CCTTATGCCT AATCATTGAA GCTCTATTTT GGAAGCAGGT CAAGAAAAAC
    CAAACCTCCT ACACACGTCA CGTTTGCATG GTGAATGTAG CCCTGTGCCT CCTGATTGCT
    GATGTTTGGT TTATTGTTGC TGCCACTGAA GGTTCCACCG TAAACCTTTC TGGAGTCTGC
    ATAGCTGCAG TGTTCTTTAT GCACTTTTTC TACCTCTCTT TGTTCTTTTG GATGCTCATG
    CTTGGCATCC TCCTGGCTTA TCGGATCCTC CTTGTGTTCC ATCACATGGC CATGCCTTTG
    ATGATGGCTG TCGGGTTCTG CCTGGGGTAT GGGTGCCCTC TCATTATATC TGTCATCACG
    ATTGCAGTCA CCCAGCCTAG CAATGCCTAC AAAAGGAAAG ATGCGTGTTG GCTTAACTGG
    TCTGATAGGA GCAAACCCTT ACTGGCCTTT GCTGTTCCCG CACTGACTAT TGTGGCTGTG
    AATTTGGCTG TGGTGCTAGT AGTTCTCACG AAGCTCTGGA GGCCCACTGT TGGAGAAAGA
    CCGGGTCAGG AAAACAAGGC CACTATTGTC CGCATGGGGA AGAGCCTCCT CATCCTGACC
    CCTCTGCTGG GGCTCACCTG GGGCTTTGGC ATAGGAACAA TAGTGGACAA CCAGAATCTG
    GCTTGGCATG TTATTTTTGC ATTACTCAAT GCATTCCAGG GTTTTTTCAT CTTATGTTTT
    GGAATGCTCT TGGACAGTAA GCTGCGACAA TTGCTGTTCA ACAAATTATC TCCTTTCAGC
    TCTTGGAAGC AAGCATCAAA GCAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA
    AAGCCTTTCA ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC
    TCAAATGACA TCATGCTAAC TCAGTTTTTA TCAACTGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292497
    Clone ID Related Accession (Same CDS sequence) XM_022426128.1
    Accession Version XM_022426128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGTAACATG CTGAGAGAAG AGAAATTTAT CACCAAAGAG
    GACCAGCCCA AGTGGGTTTG GCAGGACCTT ATCTTGCTGG GTAGGAATCA GAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281836.1
    CDS944..3760
    Translation

    Target ORF information:

    RefSeq Version XM_022426128.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGTAACATG CTGAGAGAAG AGAAATTTAT CACCAAAGAG
    GACCAGCCCA AGTGGGTTTG GCAGGACCTT ATCTTGCTGG GTAGGAATCA GAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292498
    Clone ID Related Accession (Same CDS sequence) XM_022426130.1
    Accession Version XM_022426130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGTAACATG CTGAGAGAAG AGAAATTTAT CACCAAAGAG
    GACCAGCCCA AGTGGGTTTG GCAGGACCTT ATCTTGCTGG TGGGAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281838.1
    CDS943..3753
    Translation

    Target ORF information:

    RefSeq Version XM_022426130.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGCAAGA CTCATCAGAT GTATCTGCCA AACCCAAATT CCCAAAGCCT
    TTCAACCCAT TGCAGAACAA AGGTAACATG CTGAGAGAAG AGAAATTTAT CACCAAAGAG
    GACCAGCCCA AGTGGGTTTG GCAGGACCTT ATCTTGCTGG TGGGAAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe292499
    Clone ID Related Accession (Same CDS sequence) XM_022426133.1
    Accession Version XM_022426133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G-protein coupled receptor F1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGT AACATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281841.1
    CDS943..3672
    Translation

    Target ORF information:

    RefSeq Version XM_022426133.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGT AACATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39603
    Clone ID Related Accession (Same CDS sequence) XM_014118502.1 , XM_022426132.1
    Accession Version XM_014118502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC TCAAATGACA
    TCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973977.1
    CDS3268..6036
    Translation

    Target ORF information:

    RefSeq Version XM_014118502.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC TCAAATGACA
    TCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39603
    Clone ID Related Accession (Same CDS sequence) XM_014118502.1 , XM_022426132.1
    Accession Version XM_022426132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2769bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G-protein coupled receptor F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC TCAAATGACA
    TCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281840.1
    CDS940..3708
    Translation

    Target ORF information:

    RefSeq Version XM_022426132.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022426132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAGGGTTA AAGTGCATCT GGGAAACAAG GATAAGGAGG GAGGCCAACT GAAGATGCAA 
    GTCCGCGTGC TCTGGCTCAT CTCTTTCTTC ACACTCATTG AGGGTTGGGA AGGCTTCCCG
    GGGAGAACTG AAGGCATCAA AACAAAAAGA GGACTCATTT TGAATAAGAA AAAGCATCCA
    GGCCCAGTTC GGGAGTATGA GCTACTGCTT CAGGTGTCCT ATAGAGATTC CAAGGAGAAA
    AGAGACCTGA AGAATTTTCT GAAGCTTTTG AAGCCTCCAT CATTATGGTT ACATGCTCCA
    GTGAAGATTA TTAGAGCAAA AGCCACTACA CACTGTGGCA ACCAGAATGG GAGTTTGCAG
    TGTGCCTGTG AGGACGGCTA CACCTGGTTT CCTCCCTCAT GCCTTGATCC CCAGAAATGC
    TACCTTCACA CAGCTGGAGC ACTCCAGAGC TGTCACTGTC ATGTCAACAA CCTCAGTCAG
    AGCACCAATT TCTGTGAGAG AACAAAGGTT TGGGGCACTT TCAAAATTAA TGAAAGATTT
    ACCAAAGACC TTTTGAATTC ATCTTCTCCT TCATACTCCA AATATGCGAC TGGAATTGAA
    ATTCAACTTA AGGAGGCATT CAAAAGAATT CAAGATTTTG AGTCAGTTCG TGTCACCCAA
    TTTCGTCCTT ACAGAGATGG AAGAATCTTT GCTGGGTTTG AAGTTGTGAG TTCCAGCAGC
    ACATCTGAGC TGCTGTCAGC CATTAAACAG GTGGCTAAGA AGGTGAAGGA AGGCCTTCAC
    AAGCTGTTCC CACTAGAAGA CAGCTCCTTC AGAGTATTTG CAAAAGCCCA GTGTAACAGC
    ATTGTCTTTG GATTTGGGTC CAGGAATGAT GAGTTTACTC TTCCCTGTAG CAGTGGCTAT
    ACTGGAAACA TTACGGTCAG ATGCCAGCCC TCTGGGTGGC AGGTCCTCAG GGAGATGTGT
    GTGCACTCTC AGCTGGAAGA ATTGAAGAAG AATCTCAGTG TGATGGCAGG CCATGCCACC
    GAGACAGCTG TGTCATCCTT GGTGCGAAAT CTTTCCGTCG TCATTCGGCA AAACCCATCA
    ACCACAGCTG GCAATTTGGC TTCAGTAGTG TCAATTCTGG ACAGCGTCTT GTCCCTATCA
    CTGGCAAGCC ATTTCAAGGT GTCCAATTCA ACAATGGAGG ATGTCATCAG TATAGCTGAC
    CACATCCTCA GTTCAGCATC AGTAACCAAC TGGACCGTGT TACTGCAGGA AGAACAGCAA
    GCCAGCTCGA GGTTTCTGAA GACACTAGAA AACATCAGTA GCCTCGTACC TCCAACAGCT
    CTGCCTCTGA ATTTTTCTCG GGAATTTATT GACTGGAAAG GGATTCCGGT GTCTGCAAGC
    CAACTTAAGA TGGGTTACAA CTATCAGACG GAACTGTTCC CCACAAATGC CTCCATTCCT
    GTCAAAGGCC ATGTGTTGAT TGGGTCAGAC CAATTCCGGT GGTCTCTTCC AGAAACAATT
    ATCAGCATGG CCTCGTTGAC CCTGGGGAAT ATTCTATCTA CTACTCAAAA TGGAAATGCT
    CAGGTCAATG GGCCCATGAT ATCCACGGTT ATCCAAAATT ATTCCATAAA TGAGATTTTC
    CTGACTTTTT CCAAGATAGA GTCAAATCTG AGCCAACCTC ATTGTGTGTT TTGGGATTTC
    AGTCGTTTGC AGTGGAACAA TGCGGGCTGC CACCTAGTGA ACGAAACAGC AGACACGGTG
    ATGTGCCGAT GTACTCACCT GACCTCCTTC TCCATACTGA TGTCACCCTT TGTCCCTCCT
    GCCATCATCC CCGTGGTGAA ATGGATCACC TTTGTGGGAC TGGGCATCTC CATTGGAAGT
    CTCACCTTAT GCCTAATCAT TGAAGCTCTA TTTTGGAAGC AGGTCAAGAA AAACCAAACC
    TCCTACACAC GTCACGTTTG CATGGTGAAT GTAGCCCTGT GCCTCCTGAT TGCTGATGTT
    TGGTTTATTG TTGCTGCCAC TGAAGGTTCC ACCGTAAACC TTTCTGGAGT CTGCATAGCT
    GCAGTGTTCT TTATGCACTT TTTCTACCTC TCTTTGTTCT TTTGGATGCT CATGCTTGGC
    ATCCTCCTGG CTTATCGGAT CCTCCTTGTG TTCCATCACA TGGCCATGCC TTTGATGATG
    GCTGTCGGGT TCTGCCTGGG GTATGGGTGC CCTCTCATTA TATCTGTCAT CACGATTGCA
    GTCACCCAGC CTAGCAATGC CTACAAAAGG AAAGATGCGT GTTGGCTTAA CTGGTCTGAT
    AGGAGCAAAC CCTTACTGGC CTTTGCTGTT CCCGCACTGA CTATTGTGGC TGTGAATTTG
    GCTGTGGTGC TAGTAGTTCT CACGAAGCTC TGGAGGCCCA CTGTTGGAGA AAGACCGGGT
    CAGGAAAACA AGGCCACTAT TGTCCGCATG GGGAAGAGCC TCCTCATCCT GACCCCTCTG
    CTGGGGCTCA CCTGGGGCTT TGGCATAGGA ACAATAGTGG ACAACCAGAA TCTGGCTTGG
    CATGTTATTT TTGCATTACT CAATGCATTC CAGGGTTTTT TCATCTTATG TTTTGGAATG
    CTCTTGGACA GTAAGCTGCG ACAATTGCTG TTCAACAAAT TATCTCCTTT CAGCTCTTGG
    AAGCAAGCAT CAAAGACTCA TCAGATGTAT CTGCCAAACC CAAATTCCCA AAGCCTTTCA
    ACCCATTGCA GAACAAAGGC ATTTATGTAC TTTCTCATAC TGGTGAATCC TCAAATGACA
    TCATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.