AAK1 cDNA ORF clone, Canis lupus familiaris(dog)

The following AAK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AAK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe13890 XM_005626181.3
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe13891 XM_531855.6
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291706 XM_022424835.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291707 XM_022424836.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291708 XM_022424837.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291709 XM_022424838.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291710 XM_022424839.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291711 XM_022424840.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe291712 XM_022424841.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe291713 XM_022424842.1
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe39046 XM_014117579.2
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe39047 XM_014117580.2
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe39048 XM_014117581.2
Latest version!
Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OCe13890 XM_005626181.2 Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe13891 XM_531855.5 Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe39048 XM_014117581.1 Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe39046 XM_014117579.1 Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe39047 XM_014117580.1 Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe13890
    Clone ID Related Accession (Same CDS sequence) XM_005626181.3 , XM_005626181.2
    Accession Version XM_005626181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGTCTGCAAC CACCACTCCA
    TCAGGCTCTC CTCGGACCTC CCAGCAAAAC GTTTATAATG CTTCCGAAGG TTCTACGTGG
    AATCCCTTCG ACGATGACAA TTTCTCCAAA CTCACAGCTG AAGAACTGCT GAACAAGGAC
    TTTGCCAAGC TGGGGGAAGG CAAACATCCT GAGAAGCTCG GAGGCTCTGC TGAGAGTTTG
    ATCCCAGGCT TTCAACCAAC TCCCGGTGAC GCTTTTGCCA CTCCCTCGTT TGCTGCTGGA
    ACCGCTGAGA AAAGGAAGGG TGGGCAGACT GTGGATTCCA GCCTGCCGCT TCTAAGTGTA
    TCTGATCCTT TCATTCCTCT TCAAGTACCC GATGCACCAG AAAAACTAAT TGAGGGACTC
    AAATCTCCTG ACACTTCTCT CCTGCTCCCT GACCTCTTGC CTATGACAGA TCCTTTTGGT
    AGCACTTCCG ATGCTGTAAT TGAAAAAGCC GATGTTGCTG TTGAGAGTCT CATACCAGGA
    CTGGAGCCCC CAGTGCCCCA GCGCCTTCCA TCTCAGACGG AATCTGTGAC CTCGAACCGC
    ACAGATTCTC TCACCGGGGA AGATTCCCTG ATTGATGGCT CCCTGCTTTC TAACCCTACT
    ACTGACCTTC TGGAAGAGTT TGCCCCCATA GCGATCTCTG CTCCAGCCCA TAAAGCTGCA
    GAAGATAGTA ATCTCATCTC AGGTTTTGAT GTCCCTGAGG GCTCGGACAA GGTGGCTGAA
    GATGAGTTTG ACCCTATTCC TGTATTGATA ACCAAAAACC CACAAGGTGG GCACTCTAGA
    AACAGCAGTG GGAGCTCTGA GTCCAGTCTT CCCAACCTAG CCAGGTCTTT ACTGCTGGTG
    GATCAGCTCA TAGACCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626238.1
    CDS394..3234
    Translation

    Target ORF information:

    RefSeq Version XM_005626181.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGTCTGCAAC CACCACTCCA
    TCAGGCTCTC CTCGGACCTC CCAGCAAAAC GTTTATAATG CTTCCGAAGG TTCTACGTGG
    AATCCCTTCG ACGATGACAA TTTCTCCAAA CTCACAGCTG AAGAACTGCT GAACAAGGAC
    TTTGCCAAGC TGGGGGAAGG CAAACATCCT GAGAAGCTCG GAGGCTCTGC TGAGAGTTTG
    ATCCCAGGCT TTCAACCAAC TCCCGGTGAC GCTTTTGCCA CTCCCTCGTT TGCTGCTGGA
    ACCGCTGAGA AAAGGAAGGG TGGGCAGACT GTGGATTCCA GCCTGCCGCT TCTAAGTGTA
    TCTGATCCTT TCATTCCTCT TCAAGTACCC GATGCACCAG AAAAACTAAT TGAGGGACTC
    AAATCTCCTG ACACTTCTCT CCTGCTCCCT GACCTCTTGC CTATGACAGA TCCTTTTGGT
    AGCACTTCCG ATGCTGTAAT TGAAAAAGCC GATGTTGCTG TTGAGAGTCT CATACCAGGA
    CTGGAGCCCC CAGTGCCCCA GCGCCTTCCA TCTCAGACGG AATCTGTGAC CTCGAACCGC
    ACAGATTCTC TCACCGGGGA AGATTCCCTG ATTGATGGCT CCCTGCTTTC TAACCCTACT
    ACTGACCTTC TGGAAGAGTT TGCCCCCATA GCGATCTCTG CTCCAGCCCA TAAAGCTGCA
    GAAGATAGTA ATCTCATCTC AGGTTTTGAT GTCCCTGAGG GCTCGGACAA GGTGGCTGAA
    GATGAGTTTG ACCCTATTCC TGTATTGATA ACCAAAAACC CACAAGGTGG GCACTCTAGA
    AACAGCAGTG GGAGCTCTGA GTCCAGTCTT CCCAACCTAG CCAGGTCTTT ACTGCTGGTG
    GATCAGCTCA TAGACCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13890
    Clone ID Related Accession (Same CDS sequence) XM_005626181.3 , XM_005626181.2
    Accession Version XM_005626181.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AP2-associated protein kinase 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGTCTGCAAC CACCACTCCA
    TCAGGCTCTC CTCGGACCTC CCAGCAAAAC GTTTATAATG CTTCCGAAGG TTCTACGTGG
    AATCCCTTCG ACGATGACAA TTTCTCCAAA CTCACAGCTG AAGAACTGCT GAACAAGGAC
    TTTGCCAAGC TGGGGGAAGG CAAACATCCT GAGAAGCTCG GAGGCTCTGC TGAGAGTTTG
    ATCCCAGGCT TTCAACCAAC TCCCGGTGAC GCTTTTGCCA CTCCCTCGTT TGCTGCTGGA
    ACCGCTGAGA AAAGGAAGGG TGGGCAGACT GTGGATTCCA GCCTGCCGCT TCTAAGTGTA
    TCTGATCCTT TCATTCCTCT TCAAGTACCC GATGCACCAG AAAAACTAAT TGAGGGACTC
    AAATCTCCTG ACACTTCTCT CCTGCTCCCT GACCTCTTGC CTATGACAGA TCCTTTTGGT
    AGCACTTCCG ATGCTGTAAT TGAAAAAGCC GATGTTGCTG TTGAGAGTCT CATACCAGGA
    CTGGAGCCCC CAGTGCCCCA GCGCCTTCCA TCTCAGACGG AATCTGTGAC CTCGAACCGC
    ACAGATTCTC TCACCGGGGA AGATTCCCTG ATTGATGGCT CCCTGCTTTC TAACCCTACT
    ACTGACCTTC TGGAAGAGTT TGCCCCCATA GCGATCTCTG CTCCAGCCCA TAAAGCTGCA
    GAAGATAGTA ATCTCATCTC AGGTTTTGAT GTCCCTGAGG GCTCGGACAA GGTGGCTGAA
    GATGAGTTTG ACCCTATTCC TGTATTGATA ACCAAAAACC CACAAGGTGG GCACTCTAGA
    AACAGCAGTG GGAGCTCTGA GTCCAGTCTT CCCAACCTAG CCAGGTCTTT ACTGCTGGTG
    GATCAGCTCA TAGACCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626238.1
    CDS435..3275
    Translation

    Target ORF information:

    RefSeq Version XM_005626181.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGTCTGCAAC CACCACTCCA
    TCAGGCTCTC CTCGGACCTC CCAGCAAAAC GTTTATAATG CTTCCGAAGG TTCTACGTGG
    AATCCCTTCG ACGATGACAA TTTCTCCAAA CTCACAGCTG AAGAACTGCT GAACAAGGAC
    TTTGCCAAGC TGGGGGAAGG CAAACATCCT GAGAAGCTCG GAGGCTCTGC TGAGAGTTTG
    ATCCCAGGCT TTCAACCAAC TCCCGGTGAC GCTTTTGCCA CTCCCTCGTT TGCTGCTGGA
    ACCGCTGAGA AAAGGAAGGG TGGGCAGACT GTGGATTCCA GCCTGCCGCT TCTAAGTGTA
    TCTGATCCTT TCATTCCTCT TCAAGTACCC GATGCACCAG AAAAACTAAT TGAGGGACTC
    AAATCTCCTG ACACTTCTCT CCTGCTCCCT GACCTCTTGC CTATGACAGA TCCTTTTGGT
    AGCACTTCCG ATGCTGTAAT TGAAAAAGCC GATGTTGCTG TTGAGAGTCT CATACCAGGA
    CTGGAGCCCC CAGTGCCCCA GCGCCTTCCA TCTCAGACGG AATCTGTGAC CTCGAACCGC
    ACAGATTCTC TCACCGGGGA AGATTCCCTG ATTGATGGCT CCCTGCTTTC TAACCCTACT
    ACTGACCTTC TGGAAGAGTT TGCCCCCATA GCGATCTCTG CTCCAGCCCA TAAAGCTGCA
    GAAGATAGTA ATCTCATCTC AGGTTTTGAT GTCCCTGAGG GCTCGGACAA GGTGGCTGAA
    GATGAGTTTG ACCCTATTCC TGTATTGATA ACCAAAAACC CACAAGGTGG GCACTCTAGA
    AACAGCAGTG GGAGCTCTGA GTCCAGTCTT CCCAACCTAG CCAGGTCTTT ACTGCTGGTG
    GATCAGCTCA TAGACCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13891
    Clone ID Related Accession (Same CDS sequence) XM_531855.5 , XM_531855.6
    Accession Version XM_531855.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AP2-associated protein kinase 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_531855.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGAT TCAAGCCCCA
    GTAAGACAAC AGCCAAAGGT TCAAACAACC CCACCTCCTG CCGTCCAGGG GCAGAAACTC
    GGATCTCTCA CTCCTCCATC ATCCCCCAAG ACCCAACGTG CTGGGCACAG GCGGATTCTC
    AGTGATGTAA CCCACAGCGC AGTCTTTGGG GTCCCTGCCA GCAAATCAAC CCAGCTGCTC
    CAGGCAGCTG CAGCTGAGGC CAGCCTCAAT AAGTCCAAGT CTGCAACCAC CACTCCATCA
    GGCTCTCCTC GGACCTCCCA GCAAAACGTT TATAATGCTT CCGAAGGTTC TACGTGGAAT
    CCCTTCGACG ATGACAATTT CTCCAAACTC ACAGCTGAAG AACTGCTGAA CAAGGACTTT
    GCCAAGCTGG GGGAAGGCAA ACATCCTGAG AAGCTCGGAG GCTCTGCTGA GAGTTTGATC
    CCAGGCTTTC AACCAACTCC CGGTGACGCT TTTGCCACTC CCTCGTTTGC TGCTGGAACC
    GCTGAGAAAA GGAAGGGTGG GCAGACTGTG GATTCCAGCC TGCCGCTTCT AAGTGTATCT
    GATCCTTTCA TTCCTCTTCA AGTACCCGAT GCACCAGAAA AACTAATTGA GGGACTCAAA
    TCTCCTGACA CTTCTCTCCT GCTCCCTGAC CTCTTGCCTA TGACAGATCC TTTTGGTAGC
    ACTTCCGATG CTGTAATTGA AAAAGCCGAT GTTGCTGTTG AGAGTCTCAT ACCAGGACTG
    GAGCCCCCAG TGCCCCAGCG CCTTCCATCT CAGACGGAAT CTGTGACCTC GAACCGCACA
    GATTCTCTCA CCGGGGAAGA TTCCCTGATT GATGGCTCCC TGCTTTCTAA CCCTACTACT
    GACCTTCTGG AAGAGTTTGC CCCCATAGCG ATCTCTGCTC CAGCCCATAA AGCTGCAGAA
    GATAGTAATC TCATCTCAGG TTTTGATGTC CCTGAGGGCT CGGACAAGGT GGCTGAAGAT
    GAGTTTGACC CTATTCCTGT ATTGATAACC AAAAACCCAC AAGGTGGGCA CTCTAGAAAC
    AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC AACCTAGCCA GGTCTTTACT GCTGGTGGAT
    CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_531855.3
    CDS435..3272
    Translation

    Target ORF information:

    RefSeq Version XM_531855.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_531855.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGAT TCAAGCCCCA
    GTAAGACAAC AGCCAAAGGT TCAAACAACC CCACCTCCTG CCGTCCAGGG GCAGAAACTC
    GGATCTCTCA CTCCTCCATC ATCCCCCAAG ACCCAACGTG CTGGGCACAG GCGGATTCTC
    AGTGATGTAA CCCACAGCGC AGTCTTTGGG GTCCCTGCCA GCAAATCAAC CCAGCTGCTC
    CAGGCAGCTG CAGCTGAGGC CAGCCTCAAT AAGTCCAAGT CTGCAACCAC CACTCCATCA
    GGCTCTCCTC GGACCTCCCA GCAAAACGTT TATAATGCTT CCGAAGGTTC TACGTGGAAT
    CCCTTCGACG ATGACAATTT CTCCAAACTC ACAGCTGAAG AACTGCTGAA CAAGGACTTT
    GCCAAGCTGG GGGAAGGCAA ACATCCTGAG AAGCTCGGAG GCTCTGCTGA GAGTTTGATC
    CCAGGCTTTC AACCAACTCC CGGTGACGCT TTTGCCACTC CCTCGTTTGC TGCTGGAACC
    GCTGAGAAAA GGAAGGGTGG GCAGACTGTG GATTCCAGCC TGCCGCTTCT AAGTGTATCT
    GATCCTTTCA TTCCTCTTCA AGTACCCGAT GCACCAGAAA AACTAATTGA GGGACTCAAA
    TCTCCTGACA CTTCTCTCCT GCTCCCTGAC CTCTTGCCTA TGACAGATCC TTTTGGTAGC
    ACTTCCGATG CTGTAATTGA AAAAGCCGAT GTTGCTGTTG AGAGTCTCAT ACCAGGACTG
    GAGCCCCCAG TGCCCCAGCG CCTTCCATCT CAGACGGAAT CTGTGACCTC GAACCGCACA
    GATTCTCTCA CCGGGGAAGA TTCCCTGATT GATGGCTCCC TGCTTTCTAA CCCTACTACT
    GACCTTCTGG AAGAGTTTGC CCCCATAGCG ATCTCTGCTC CAGCCCATAA AGCTGCAGAA
    GATAGTAATC TCATCTCAGG TTTTGATGTC CCTGAGGGCT CGGACAAGGT GGCTGAAGAT
    GAGTTTGACC CTATTCCTGT ATTGATAACC AAAAACCCAC AAGGTGGGCA CTCTAGAAAC
    AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC AACCTAGCCA GGTCTTTACT GCTGGTGGAT
    CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13891
    Clone ID Related Accession (Same CDS sequence) XM_531855.5 , XM_531855.6
    Accession Version XM_531855.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_531855.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGAT TCAAGCCCCA
    GTAAGACAAC AGCCAAAGGT TCAAACAACC CCACCTCCTG CCGTCCAGGG GCAGAAACTC
    GGATCTCTCA CTCCTCCATC ATCCCCCAAG ACCCAACGTG CTGGGCACAG GCGGATTCTC
    AGTGATGTAA CCCACAGCGC AGTCTTTGGG GTCCCTGCCA GCAAATCAAC CCAGCTGCTC
    CAGGCAGCTG CAGCTGAGGC CAGCCTCAAT AAGTCCAAGT CTGCAACCAC CACTCCATCA
    GGCTCTCCTC GGACCTCCCA GCAAAACGTT TATAATGCTT CCGAAGGTTC TACGTGGAAT
    CCCTTCGACG ATGACAATTT CTCCAAACTC ACAGCTGAAG AACTGCTGAA CAAGGACTTT
    GCCAAGCTGG GGGAAGGCAA ACATCCTGAG AAGCTCGGAG GCTCTGCTGA GAGTTTGATC
    CCAGGCTTTC AACCAACTCC CGGTGACGCT TTTGCCACTC CCTCGTTTGC TGCTGGAACC
    GCTGAGAAAA GGAAGGGTGG GCAGACTGTG GATTCCAGCC TGCCGCTTCT AAGTGTATCT
    GATCCTTTCA TTCCTCTTCA AGTACCCGAT GCACCAGAAA AACTAATTGA GGGACTCAAA
    TCTCCTGACA CTTCTCTCCT GCTCCCTGAC CTCTTGCCTA TGACAGATCC TTTTGGTAGC
    ACTTCCGATG CTGTAATTGA AAAAGCCGAT GTTGCTGTTG AGAGTCTCAT ACCAGGACTG
    GAGCCCCCAG TGCCCCAGCG CCTTCCATCT CAGACGGAAT CTGTGACCTC GAACCGCACA
    GATTCTCTCA CCGGGGAAGA TTCCCTGATT GATGGCTCCC TGCTTTCTAA CCCTACTACT
    GACCTTCTGG AAGAGTTTGC CCCCATAGCG ATCTCTGCTC CAGCCCATAA AGCTGCAGAA
    GATAGTAATC TCATCTCAGG TTTTGATGTC CCTGAGGGCT CGGACAAGGT GGCTGAAGAT
    GAGTTTGACC CTATTCCTGT ATTGATAACC AAAAACCCAC AAGGTGGGCA CTCTAGAAAC
    AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC AACCTAGCCA GGTCTTTACT GCTGGTGGAT
    CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_531855.3
    CDS394..3231
    Translation

    Target ORF information:

    RefSeq Version XM_531855.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_531855.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGAT TCAAGCCCCA
    GTAAGACAAC AGCCAAAGGT TCAAACAACC CCACCTCCTG CCGTCCAGGG GCAGAAACTC
    GGATCTCTCA CTCCTCCATC ATCCCCCAAG ACCCAACGTG CTGGGCACAG GCGGATTCTC
    AGTGATGTAA CCCACAGCGC AGTCTTTGGG GTCCCTGCCA GCAAATCAAC CCAGCTGCTC
    CAGGCAGCTG CAGCTGAGGC CAGCCTCAAT AAGTCCAAGT CTGCAACCAC CACTCCATCA
    GGCTCTCCTC GGACCTCCCA GCAAAACGTT TATAATGCTT CCGAAGGTTC TACGTGGAAT
    CCCTTCGACG ATGACAATTT CTCCAAACTC ACAGCTGAAG AACTGCTGAA CAAGGACTTT
    GCCAAGCTGG GGGAAGGCAA ACATCCTGAG AAGCTCGGAG GCTCTGCTGA GAGTTTGATC
    CCAGGCTTTC AACCAACTCC CGGTGACGCT TTTGCCACTC CCTCGTTTGC TGCTGGAACC
    GCTGAGAAAA GGAAGGGTGG GCAGACTGTG GATTCCAGCC TGCCGCTTCT AAGTGTATCT
    GATCCTTTCA TTCCTCTTCA AGTACCCGAT GCACCAGAAA AACTAATTGA GGGACTCAAA
    TCTCCTGACA CTTCTCTCCT GCTCCCTGAC CTCTTGCCTA TGACAGATCC TTTTGGTAGC
    ACTTCCGATG CTGTAATTGA AAAAGCCGAT GTTGCTGTTG AGAGTCTCAT ACCAGGACTG
    GAGCCCCCAG TGCCCCAGCG CCTTCCATCT CAGACGGAAT CTGTGACCTC GAACCGCACA
    GATTCTCTCA CCGGGGAAGA TTCCCTGATT GATGGCTCCC TGCTTTCTAA CCCTACTACT
    GACCTTCTGG AAGAGTTTGC CCCCATAGCG ATCTCTGCTC CAGCCCATAA AGCTGCAGAA
    GATAGTAATC TCATCTCAGG TTTTGATGTC CCTGAGGGCT CGGACAAGGT GGCTGAAGAT
    GAGTTTGACC CTATTCCTGT ATTGATAACC AAAAACCCAC AAGGTGGGCA CTCTAGAAAC
    AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC AACCTAGCCA GGTCTTTACT GCTGGTGGAT
    CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291706
    Clone ID Related Accession (Same CDS sequence) XM_022424835.1
    Accession Version XM_022424835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGAGACTA TAATCTTACC CACACGAAGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280543.1
    CDS395..3187
    Translation

    Target ORF information:

    RefSeq Version XM_022424835.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGAGACTA TAATCTTACC CACACGAAGT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291707
    Clone ID Related Accession (Same CDS sequence) XM_022424836.1
    Accession Version XM_022424836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATGGCTCCCT GCTTTCTAAC CCTACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCGA TCTCTGCTCC AGCCCATAAA GCTGCAGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCTGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTACTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280544.1
    CDS395..3151
    Translation

    Target ORF information:

    RefSeq Version XM_022424836.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT
    TCCCTGATTG ATGGCTCCCT GCTTTCTAAC CCTACTACTG ACCTTCTGGA AGAGTTTGCC
    CCCATAGCGA TCTCTGCTCC AGCCCATAAA GCTGCAGAAG ATAGTAATCT CATCTCAGGT
    TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCTGAAGATG AGTTTGACCC TATTCCTGTA
    TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC
    AGTCTTCCCA ACCTAGCCAG GTCTTTACTG CTGGTGGATC AGCTCATAGA CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291708
    Clone ID Related Accession (Same CDS sequence) XM_022424837.1
    Accession Version XM_022424837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGAGACTAT AATCTTACCC ACACGAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280545.1
    CDS396..3077
    Translation

    Target ORF information:

    RefSeq Version XM_022424837.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGAGACTAT AATCTTACCC ACACGAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291709
    Clone ID Related Accession (Same CDS sequence) XM_022424838.1
    Accession Version XM_022424838.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCAGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CTGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTGGGCACT CTAGAAACAG CAGTGGGAGC
    TCTGAGTCCA GTCTTCCCAA CCTAGCCAGG TCTTTACTGC TGGTGGATCA GCTCATAGAC
    CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280546.1
    CDS396..3041
    Translation

    Target ORF information:

    RefSeq Version XM_022424838.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCAGAAGA TAGTAATCTC
    ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CTGAAGATGA GTTTGACCCT
    ATTCCTGTAT TGATAACCAA AAACCCACAA GGTGGGCACT CTAGAAACAG CAGTGGGAGC
    TCTGAGTCCA GTCTTCCCAA CCTAGCCAGG TCTTTACTGC TGGTGGATCA GCTCATAGAC
    CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291710
    Clone ID Related Accession (Same CDS sequence) XM_022424839.1
    Accession Version XM_022424839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGAGACTATA ATCTTACCCA CACGAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280547.1
    CDS396..2966
    Translation

    Target ORF information:

    RefSeq Version XM_022424839.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGAGACTATA ATCTTACCCA CACGAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291711
    Clone ID Related Accession (Same CDS sequence) XM_022424840.1
    Accession Version XM_022424840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2565bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGGTTCCCTC CACCTCCTTC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280548.1
    CDS396..2960
    Translation

    Target ORF information:

    RefSeq Version XM_022424840.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGGTTCCCTC CACCTCCTTC CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291712
    Clone ID Related Accession (Same CDS sequence) XM_022424841.1
    Accession Version XM_022424841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTTCCCTCCA CCTCCTTCCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280549.1
    CDS396..2858
    Translation

    Target ORF information:

    RefSeq Version XM_022424841.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAAGCCGATG TTGCTGTTGA GAGTCTCATA CCAGGACTGG AGCCCCCAGT GCCCCAGCGC
    CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTTCCCTCCA CCTCCTTCCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291713
    Clone ID Related Accession (Same CDS sequence) XM_022424842.1
    Accession Version XM_022424842.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAG
    ACTATAATCT TACCCACACG AAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280550.1
    CDS396..2762
    Translation

    Target ORF information:

    RefSeq Version XM_022424842.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAG
    ACTATAATCT TACCCACACG AAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39046
    Clone ID Related Accession (Same CDS sequence) XM_014117579.1 , XM_014117579.2
    Accession Version XM_014117579.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AP2-associated protein kinase 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG AGCTCTGAGT CCAGTCTTCC CAACCTAGCC
    AGGTCTTTAC TGCTGGTGGA TCAGCTCATA GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973054.1
    CDS435..3293
    Translation

    Target ORF information:

    RefSeq Version XM_014117579.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG AGCTCTGAGT CCAGTCTTCC CAACCTAGCC
    AGGTCTTTAC TGCTGGTGGA TCAGCTCATA GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39046
    Clone ID Related Accession (Same CDS sequence) XM_014117579.1 , XM_014117579.2
    Accession Version XM_014117579.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG AGCTCTGAGT CCAGTCTTCC CAACCTAGCC
    AGGTCTTTAC TGCTGGTGGA TCAGCTCATA GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973054.1
    CDS395..3253
    Translation

    Target ORF information:

    RefSeq Version XM_014117579.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGATTCTCTC ACCGGGGAAG ATTCCCTGAT TGATGGCTCC
    CTGCTTTCTA ACCCTACTAC TGACCTTCTG GAAGAGTTTG CCCCCATAGC GATCTCTGCT
    CCAGCCCATA AAGCTGCAGA AGATAGTAAT CTCATCTCAG GTTTTGATGT CCCTGAGGGC
    TCGGACAAGG TGGCTGAAGA TGAGTTTGAC CCTATTCCTG TATTGATAAC CAAAAACCCA
    CAAGGTGGGC ACTCTAGAAA CAGCAGTGGG AGCTCTGAGT CCAGTCTTCC CAACCTAGCC
    AGGTCTTTAC TGCTGGTGGA TCAGCTCATA GACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39047
    Clone ID Related Accession (Same CDS sequence) XM_014117580.1 , XM_014117580.2
    Accession Version XM_014117580.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AP2-associated protein kinase 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTGGGCA CTCTAGAAAC AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC
    AACCTAGCCA GGTCTTTACT GCTGGTGGAT CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973055.1
    CDS435..3182
    Translation

    Target ORF information:

    RefSeq Version XM_014117580.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTGGGCA CTCTAGAAAC AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC
    AACCTAGCCA GGTCTTTACT GCTGGTGGAT CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39047
    Clone ID Related Accession (Same CDS sequence) XM_014117580.1 , XM_014117580.2
    Accession Version XM_014117580.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTGGGCA CTCTAGAAAC AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC
    AACCTAGCCA GGTCTTTACT GCTGGTGGAT CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973055.1
    CDS396..3143
    Translation

    Target ORF information:

    RefSeq Version XM_014117580.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117580.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGAA
    AAACTAATTG AGGGACTCAA ATCTCCTGAC ACTTCTCTCC TGCTCCCTGA CCTCTTGCCT
    ATGACAGATC CTTTTGGTAG CACTTCCGAT GCTGTAATTG AAAAAGCCGA TGTTGCTGTT
    GAGAGTCTCA TACCAGGACT GGAGCCCCCA GTGCCCCAGC GCCTTCCATC TCAGACGGAA
    TCTGTGACCT CGAACCGCAC AGCTGCAGAA GATAGTAATC TCATCTCAGG TTTTGATGTC
    CCTGAGGGCT CGGACAAGGT GGCTGAAGAT GAGTTTGACC CTATTCCTGT ATTGATAACC
    AAAAACCCAC AAGGTGGGCA CTCTAGAAAC AGCAGTGGGA GCTCTGAGTC CAGTCTTCCC
    AACCTAGCCA GGTCTTTACT GCTGGTGGAT CAGCTCATAG ACCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39048
    Clone ID Related Accession (Same CDS sequence) XM_014117581.2 , XM_014117581.1
    Accession Version XM_014117581.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product AP2-associated protein kinase 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGCT
    CCCAAATACC AGACTGCCAG CTCCATAGCC TTCCATCTGT GCCTCTGCCA ACTCCAGCCA
    GGCCTCCCAT CTGCTCTTAG AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973056.1
    CDS396..2822
    Translation

    Target ORF information:

    RefSeq Version XM_014117581.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117581.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGCT
    CCCAAATACC AGACTGCCAG CTCCATAGCC TTCCATCTGT GCCTCTGCCA ACTCCAGCCA
    GGCCTCCCAT CTGCTCTTAG AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39048
    Clone ID Related Accession (Same CDS sequence) XM_014117581.2 , XM_014117581.1
    Accession Version XM_014117581.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2427bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product AP2-associated protein kinase 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGCT
    CCCAAATACC AGACTGCCAG CTCCATAGCC TTCCATCTGT GCCTCTGCCA ACTCCAGCCA
    GGCCTCCCAT CTGCTCTTAG AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973056.1
    CDS435..2861
    Translation

    Target ORF information:

    RefSeq Version XM_014117581.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117581.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CCGGCCTGGG CTCCGGCTCC 
    AGCGGAGGCG GGGGCAGCAC CTCGGGCCTG GGCAGCGGCT ACATCGGGAG GGTCTTCGGC
    ATCGGGCGGC AGCAGGTCAC GGTGGACGAG GTGCTGGCGG AAGGTGGATT TGCTATCGTG
    TTTCTGGTGA GGACAAGCAA TGGAATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT
    GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGAGACCT GTCGGGGCAC
    AAGAACATTG TCGGTTACAT CGACTCCAGT ATCAACAATG TGAGCAGCGG TGATGTATGG
    GAGGTGCTCA TCCTGATGGA CTTTTGTAGG GGAGGCCAGG TGGTCAACCT CATGAACCAG
    CGCCTGCAAA CAGGCTTCAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA CACCTGCGAA
    GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGTGACCT GAAGGTTGAA
    AATATCCTCT TGCATGACCG AGGCCACTAC GTCCTGTGTG ACTTTGGAAG CGCCACCAAC
    AAATTTCAGA ATCCACAAAC TGAGGGCGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC
    ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT
    ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG
    CCATTTGGAG AGAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAACTCT
    CGATATTCCC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGATAAA
    AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAAC TCCTCAAGAA AGAATGCCCA
    ATTCCTAATG TACAGAACTC TCCCATTCCT GCCAAGCTTC CTGAGCCAGT GAAAGCCAGT
    GAGGCAGCTG CAAAAAAGAG CCAGCCGAAG GCCAGACTGA CGGATCCCAT TCCCACCACA
    GAGACTTCAA TCGCACCCCG TCAGAGGCCT AAGGCTGGGC AGACTCAGCC AAACCCAGGA
    ATCCTTCCTA TCCAGCCAGC CCTGACACCT AGAAAGCGGG CCACGGTTCA GCCCCCACCC
    CAGGCTGCAG GCTCCGGCAG TCAGCCTGGC CTTTTAGCCA GCGTCCCCCA ACCGAAACCC
    CAGGCCCAAC CCAGCCAGCC TCTACCCCAG CCTCAGCCCA AGCAGCCCCA GGCTCCCCCG
    GCCCCGCAGC AGACGCCGGC TCCGGCGCAG GCCACACCGC AGCACCAGCA GCCGCTCTTC
    CTCAAGCAGC CGCCCCAGCA GCAGCCGCCG CCCCAGCAGC CGCCGCCCCA GCAGCCCGCG
    GGCACGTTCT ACCAGCAGCC GCCGCCGCAG GCCCAGGCCC AGGCCCAGCA GTTTCAGGCC
    GTACACCCCG CAGCCCAGCA GCCCGCGATC GCCCCGTTCC CCGTGGCGCC CCAAGGAAGC
    TCTCAGCAGC CCCTGATCCA GAACTTCTAC CAGCAGCCGC AGCAGCCGCC GCTGGCCACG
    GGCCTGCACC AGCCGCAGCT GCTGACCCAG AAGGCCGCCG CCGCCGCGCC GCAGCCCCCC
    GCAGCCCCGG CCGCAGCCCC GCAGCCGCCC CCGGCCCAGG AGCCCCCGCA GATTCAAGCC
    CCAGTAAGAC AACAGCCAAA GGTTCAAACA ACCCCACCTC CTGCCGTCCA GGGGCAGAAA
    CTCGGATCTC TCACTCCTCC ATCATCCCCC AAGACCCAAC GTGCTGGGCA CAGGCGGATT
    CTCAGTGATG TAACCCACAG CGCAGTCTTT GGGGTCCCTG CCAGCAAATC AACCCAGCTG
    CTCCAGGCAG CTGCAGCTGA GGCCAGCCTC AATAAGTCCA AGGGTGGGAA CCTACCTAGG
    TCTGCAACCA CCACTCCATC AGGCTCTCCT CGGACCTCCC AGCAAAACGT TTATAATGCT
    TCCGAAGGTT CTACGTGGAA TCCCTTCGAC GATGACAATT TCTCCAAACT CACAGCTGAA
    GAACTGCTGA ACAAGGACTT TGCCAAGCTG GGGGAAGGCA AACATCCTGA GAAGCTCGGA
    GGCTCTGCTG AGAGTTTGAT CCCAGGCTTT CAACCAACTC CCGGTGACGC TTTTGCCACT
    CCCTCGTTTG CTGCTGGAAC CGCTGAGAAA AGGAAGGGTG GGCAGACTGT GGATTCCAGC
    CTGCCGCTTC TAAGTGTATC TGATCCTTTC ATTCCTCTTC AAGTACCCGA TGCACCAGCT
    CCCAAATACC AGACTGCCAG CTCCATAGCC TTCCATCTGT GCCTCTGCCA ACTCCAGCCA
    GGCCTCCCAT CTGCTCTTAG AGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.