CSF2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSF2
Entrez Gene ID	403923
Full Name	colony stimulating factor 2
Synonyms	GM-CSF
General protein information	Preferred Names colony stimulating factor 2 Names granulocyte-macrophage colony-stimulating factor CSF colony-stimulating factor
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005626371.1	XP_005626428.1	granulocyte-macrophage colony-stimulating factor isoform X1
XM_022424895.1	XP_022280603.1	granulocyte-macrophage colony-stimulating factor isoform X1
XM_022424896.1	XP_022280604.1	granulocyte-macrophage colony-stimulating factor isoform X1
NM_001003245.1	NP_001003245.1	granulocyte-macrophage colony-stimulating factor precursor
XM_005626372.2	XP_005626429.1	granulocyte-macrophage colony-stimulating factor isoform X1
XM_005626371.2	XP_005626428.1	granulocyte-macrophage colony-stimulating factor isoform X1
XM_022424894.1	XP_022280602.1	granulocyte-macrophage colony-stimulating factor isoform X1
XM_005626372.1	XP_005626429.1	granulocyte-macrophage colony-stimulating factor isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CSF2	XP_527005.2
Bos taurus (cattle)	CSF2	NP_776452.1
Mus musculus (house mouse)	Csf2	NP_034099.2
Rattus norvegicus (Norway rat)	Csf2	NP_446304.1
Homo sapiens (human)	CSF2	NP_000749.2
Canis lupus familiaris (dog)	CSF2	NP_001003245.1
	CSF2	NP_001028121.1

Gene Ontology
Bibliography

Related articles in PubMed

MCP-1, KC-like and IL-8 as critical mediators of pathogenesis caused by Babesia canis.
Gal?n A, Mayer I, Rafaj RB, Bendelja K, Su?i? V, Cer?n JJ, Mrljak V
PloS one13(1)e0190474(2018)

Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease.
Kimura T, Sekido M, Iio A, Chimura N, Shibata S, Kamishina H, Kamishina H, Maeda S
Veterinary dermatology24(3)310-4, e69(2013 Jun)

Effect of recombinant canine interferon-? on granulocyte-macrophage colony-stimulating factor, transforming growth factor-? and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK).
Shibata S, Maeda S, Kondo N, Inoue A, Maeda S, Chimura N, Fukata T
Veterinary dermatology22(1)24-30(2011 Feb)

Molecular cloning and in vivo evaluation of canine granulocyte-macrophage colony-stimulating factor.
Nash RA, Schuening F, Appelbaum F, Hammond WP, Boone T, Morris CF, Slichter SJ, Storb R
Blood78(4)930-7(1991 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that GM-CSF production mediated by the cysteine protease is regulated not only by NFAT but also by unknown signalling pathways in canine keratinocytes.
Title: Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease.

This study suggests that interferon-gamma may suppress granulocyte-macrophage colony stimulating factor production from canine keratinocytes, although further studies are required to confirm this.
Title: Effect of recombinant canine interferon-? on granulocyte-macrophage colony-stimulating factor, transforming growth factor-? and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK).

The following CSF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe14126	NM_001003245.1 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_005626371.2 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_022424896.1 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_005626372.2 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_022424895.1 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_022424894.1 Latest version!	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OCe14126	XM_005626371.1	Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe14126	XM_005626372.1	Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSF2 ( NM_001003245.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001003245.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	NM_001003245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S49738.1. ##Evidence-Data-START## Transcript exon combination :: S49738.1, BM539160.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547738, SAMEA4547740 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003245.1
CDS	7..441
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	NM_001003245.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003245.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_005626371.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626428.1 Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_005626371.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626428.1
CDS	1396..1830
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_005626371.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626371.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_022424895.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022280603.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_022424895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280603.1
CDS	945..1379
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_022424895.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424895.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_022424896.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022280604.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_022424896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280604.1
CDS	927..1361
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_022424896.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424896.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_005626372.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626429.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_005626372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626429.1
CDS	1307..1741
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_005626372.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626372.2

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_005626371.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626428.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_005626371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626428.1
CDS	1412..1846
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_005626371.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626371.2

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_022424894.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022280602.1 Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_022424894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280602.1
CDS	1430..1864
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_022424894.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (CSF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424894.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSF2 ( XM_005626372.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005626429.1 Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14126
Clone ID Related Accession (Same CDS sequence)	XM_005626371.1 , XM_022424895.1 , XM_022424896.1 , NM_001003245.1 , XM_005626372.2 , XM_005626371.2 , XM_022424894.1 , XM_005626372.1
Accession Version	XM_005626372.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	granulocyte-macrophage colony-stimulating factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626429.1
CDS	1291..1725
Translation	MWLQNLLFLGTVVCSISAPTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Target ORF information:

RefSeq Version	XM_005626372.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris colony stimulating factor 2 (granulocyte-macrophage) (CSF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626372.1

ORF Insert Sequence:

ATGTGGCTGC AGAACCTGCT TTTCTTGGGC ACTGTGGTCT GCAGCATCTC TGCACCCACC 
CGCTCACCCA CCCTTGTCAC TCGGCCCTCT CAGCACGTGG ATGCCATCCA GGAAGCCCTG 
AGCCTTTTGA ACAACAGTAA TGACGTGACT GCTGTGATGA ATAAAGCAGT AAAAGTGGTC 
TCTGAAGTGT TTGACCCTGA GGGGCCAACA TGCCTGGAGA CCCGCCTACA GCTGTACAAG 
GAGGGCCTGC AGGGCAGCCT CACCAGCCTC AAGAATCCCT TAACCATGAT GGCCAATCAC 
TATAAGCAGC ACTGTCCCCC TACCCCGGAA TCTCCCTGTG CAACCCAGAA TATTAACTTC 
AAAAGTTTCA AAGAGAACCT GAAGGATTTT CTGTTTAACA TCCCCTTTGA CTGCTGGAAA 
CCAGTCAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MCP-1, KC-like and IL-8 as critical mediators of pathogenesis caused by Babesia canis.
PloS one13(1)e0190474(2018)
Gal?n A,Mayer I,Rafaj RB,Bendelja K,Su?i? V,Cer?n JJ,Mrljak V

Involvement of nuclear factor of activated T cells in granulocyte-macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease.
Veterinary dermatology24(3)310-4, e69(2013 Jun)
Kimura T,Sekido M,Iio A,Chimura N,Shibata S,Kamishina H,Kamishina H,Maeda S

Effect of recombinant canine interferon-? on granulocyte-macrophage colony-stimulating factor, transforming growth factor-? and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK).
Veterinary dermatology22(1)24-30(2011 Feb)
Shibata S,Maeda S,Kondo N,Inoue A,Maeda S,Chimura N,Fukata T

Molecular cloning and in vivo evaluation of canine granulocyte-macrophage colony-stimulating factor.
Blood78(4)930-7(1991 Aug)
Nash RA,Schuening F,Appelbaum F,Hammond WP,Boone T,Morris CF,Slichter SJ,Storb R

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CSF2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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