RLN2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RLN2
Entrez Gene ID	403742
Full Name	relaxin 2
Synonyms	RLN,RLN1,relaxin
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003132.1	NP_001003132.1	prorelaxin precursor
XM_014113974.1	XP_013969449.1	prorelaxin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RLN2	NP_604390.1
	RLN2	NP_001108598.1
Canis lupus familiaris (dog)	RLN1	NP_001003132.1
Pan troglodytes (chimpanzee)	RLN2	NP_001116246.1

Gene Ontology
Bibliography

Related articles in PubMed

Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.
Wolf K, Kayacelebi H, Urhausen C, Piechotta M, Mischke R, Kramer S, Einspanier A, Oei CH, G?nzel-Apel A
Reproduction in domestic animals = Zuchthygiene47 Suppl 6243-6(2012 Dec)

Investigation of the local expression of the relaxin system in canine mammary tumours.
Lamp O, Honscha KU, Jakob J, Lamp J, Schweizer S, Reischauer A, Gottschalk J, Hahn A, Ebert M, Rothemund S, Blaschzik S, Einspanier A
Reproduction in domestic animals = Zuchthygiene44 Suppl 2224-9(2009 Jul)

Canine preprorelaxin: nucleic acid sequence and localization within the canine placenta.
Klonisch T, Hombach-Klonisch S, Froehlich C, Kauffold J, Steger K, Steinetz BG, Fischer B
Biology of reproduction60(3)551-7(1999 Mar)

Purification and sequence determination of canine relaxin.
Stewart DR, Henzel WJ, Vandlen R
Journal of protein chemistry11(3)247-53(1992 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The blood levels of multiple steroids and other hormones in adult male dogs with or without prostatic hyperplasia are reported.
Title: Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.

The expression of relaxin and its receptors in canine mammary neoplasm tissue were studied, indicating a possible significant role of relaxin as an inducer of connective tissue remodelling.
Title: Investigation of the local expression of the relaxin system in canine mammary tumours.

The following RLN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RLN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33845	NM_001003132.1 Latest version!	Canis lupus familiaris relaxin 2 (RLN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe34544	XM_014113974.1 Latest version!	Canis lupus familiaris relaxin 2 (RLN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RLN2 ( NM_001003132.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001003132.1 Canis lupus familiaris relaxin 2 (RLN2), mRNA.

Price & Availability↑

CloneID	OCe33845
Clone ID Related Accession (Same CDS sequence)	NM_001003132.1
Accession Version	NM_001003132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Canis lupus familiaris(dog)
Product	prorelaxin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF233687.1. ##Evidence-Data-START## Transcript exon combination :: AF233687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCGCT GGTTCTTGTC CCACCTGCTA GGTGTCTGGC TGCTACTAAG CCAACTTCCC 
AGAGAGATCC CAGCCACGGA TGACAAGAAA CTTAAGGCAT GTGGTCGTGA TTATGTCCGC 
CTACAGATTG AGGTCTGCGG CTCCATCTGG TGGGGGAGAA AGGCTGGCCA GCTGCGGGAA 
CGTCGGCAGA TATCCGAACC CCTGGCAGAA GTTGTGCCAT CCTCCATCAT CAATGATCCA 
GAAATCTTAA GTTTGATGTT GCAATCCATT CCCGGTATGC CACAGGAACT GAGGATAGCA 
ACACGGTCTG GGAAGGAGAA GTTATTAAGA GAGCTACACT TTGTACTGGA AGATTCCAAT 
CTTAACTTGG AAGAAATGAA GAAAACTTTT CTTAACACAC AATTTGAAGC TGAAGACAAA 
AGCCTTTCAA AATTAGATAA ACATCCCCGA AAAAAGAGAG ATAACTACAT AAAAATGAGT 
GATAAATGTT GTAATGTAGG TTGTACCAGA AGAGAGCTTG CTAGCCGATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003132.1
CDS	1..534
Translation	MLRWFLSHLLGVWLLLSQLPREIPATDDKKLKACGRDYVRLQIEVCGSIWWGRKAGQLRERRQISEPLAEVVPSSIINDPEILSLMLQSIPGMPQELRIATRSGKEKLLRELHFVLEDSNLNLEEMKKTFLNTQFEAEDKSLSKLDKHPRKKRDNYIKMSDKCCNVGCTRRELASRC

Target ORF information:

RefSeq Version	NM_001003132.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris relaxin 2 (RLN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003132.1

ORF Insert Sequence:

ATGCTGCGCT GGTTCTTGTC CCACCTGCTA GGTGTCTGGC TGCTACTAAG CCAACTTCCC 
AGAGAGATCC CAGCCACGGA TGACAAGAAA CTTAAGGCAT GTGGTCGTGA TTATGTCCGC 
CTACAGATTG AGGTCTGCGG CTCCATCTGG TGGGGGAGAA AGGCTGGCCA GCTGCGGGAA 
CGTCGGCAGA TATCCGAACC CCTGGCAGAA GTTGTGCCAT CCTCCATCAT CAATGATCCA 
GAAATCTTAA GTTTGATGTT GCAATCCATT CCCGGTATGC CACAGGAACT GAGGATAGCA 
ACACGGTCTG GGAAGGAGAA GTTATTAAGA GAGCTACACT TTGTACTGGA AGATTCCAAT 
CTTAACTTGG AAGAAATGAA GAAAACTTTT CTTAACACAC AATTTGAAGC TGAAGACAAA 
AGCCTTTCAA AATTAGATAA ACATCCCCGA AAAAAGAGAG ATAACTACAT AAAAATGAGT 
GATAAATGTT GTAATGTAGG TTGTACCAGA AGAGAGCTTG CTAGCCGATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RLN2 ( XM_014113974.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013969449.1 Canis lupus familiaris relaxin 2 (RLN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe34544
Clone ID Related Accession (Same CDS sequence)	XM_014113974.1
Accession Version	XM_014113974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	prorelaxin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT GGTTCTTGTC CCACCTGCTA GGTGTCTGGC TGCTACTAAG CCAACTTCCC 
AGAGAGATCC CAGCCACGGA TGACAAGAAA CTTAAGGCAT GTGGTCGTGA TTATGTCCGC 
CTACAGATTG AGGTCTGCGG CTCCAGCTGG TGGGGGAGAA AGGCTGGCCA GCTGCGGGAA 
CGTCGGCAGA TATCCGAACC CCTGGCAGAA GTTGTGCCAT CCTCCATCAT CAATGATCCA 
GAAATCTTAA GTTTGATGTT GCAATCCATT CCCGGTATGC CACAGGAACT GAGGATAGCA 
ACACAGTCTG GGAAGGAGAA GTTATTAAGA GAGCTACACT TTGTACTGGA AGATTCCAAT 
CTTAACTTGG AAGAAATGAA GAAAACTTTT CTTAACACAC AATTTGAAGC TGAAGACAAA 
AGCCTTTCAA AATTAGATAA ACATCCCCGA AAAAAGAGAG ATAACTACAT AAAAATGAGT 
GATAAATGTT GTAATGTAGG TTGTACCAGA AGAGAGCTTG CTAGCCGATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969449.1
CDS	87..620
Translation	MLRWFLSHLLGVWLLLSQLPREIPATDDKKLKACGRDYVRLQIEVCGSSWWGRKAGQLRERRQISEPLAEVVPSSIINDPEILSLMLQSIPGMPQELRIATQSGKEKLLRELHFVLEDSNLNLEEMKKTFLNTQFEAEDKSLSKLDKHPRKKRDNYIKMSDKCCNVGCTRRELASRC

Target ORF information:

RefSeq Version	XM_014113974.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris relaxin 2 (RLN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113974.1

ORF Insert Sequence:

ATGCTGCGCT GGTTCTTGTC CCACCTGCTA GGTGTCTGGC TGCTACTAAG CCAACTTCCC 
AGAGAGATCC CAGCCACGGA TGACAAGAAA CTTAAGGCAT GTGGTCGTGA TTATGTCCGC 
CTACAGATTG AGGTCTGCGG CTCCAGCTGG TGGGGGAGAA AGGCTGGCCA GCTGCGGGAA 
CGTCGGCAGA TATCCGAACC CCTGGCAGAA GTTGTGCCAT CCTCCATCAT CAATGATCCA 
GAAATCTTAA GTTTGATGTT GCAATCCATT CCCGGTATGC CACAGGAACT GAGGATAGCA 
ACACAGTCTG GGAAGGAGAA GTTATTAAGA GAGCTACACT TTGTACTGGA AGATTCCAAT 
CTTAACTTGG AAGAAATGAA GAAAACTTTT CTTAACACAC AATTTGAAGC TGAAGACAAA 
AGCCTTTCAA AATTAGATAA ACATCCCCGA AAAAAGAGAG ATAACTACAT AAAAATGAGT 
GATAAATGTT GTAATGTAGG TTGTACCAGA AGAGAGCTTG CTAGCCGATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Testicular steroids, prolactin, relaxin and prostate gland markers in peripheral blood and seminal plasma of normal dogs and dogs with prostatic hyperplasia.
Reproduction in domestic animals = Zuchthygiene47 Suppl 6243-6(2012 Dec)
Wolf K,Kayacelebi H,Urhausen C,Piechotta M,Mischke R,Kramer S,Einspanier A,Oei CH,G?nzel-Apel A

Investigation of the local expression of the relaxin system in canine mammary tumours.
Reproduction in domestic animals = Zuchthygiene44 Suppl 2224-9(2009 Jul)
Lamp O,Honscha KU,Jakob J,Lamp J,Schweizer S,Reischauer A,Gottschalk J,Hahn A,Ebert M,Rothemund S,Blaschzik S,Einspanier A

Canine preprorelaxin: nucleic acid sequence and localization within the canine placenta.
Biology of reproduction60(3)551-7(1999 Mar)
Klonisch T,Hombach-Klonisch S,Froehlich C,Kauffold J,Steger K,Steinetz BG,Fischer B

Purification and sequence determination of canine relaxin.
Journal of protein chemistry11(3)247-53(1992 Jun)
Stewart DR,Henzel WJ,Vandlen R

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RLN2 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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