nep-21 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nep-21
Entrez Gene ID	179925
Full Name	NEPrilysin metallopeptidase family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356755.2	NP_001343820.1	Neprilysin-21
NM_074119.5	NP_506520.1	Neprilysin-21
NM_074119.4	NP_506520.1	Neprilysin-21
NM_001276955.1	NP_001263884.1	Neprilysin-21
NM_001356755.1	NP_001343820.1	Neprilysin-21
NM_001373156.1	NP_001360525.1	Neprilysin-21
NM_001276955.2	NP_001263884.1	Neprilysin-21

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nep-21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nep-21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa345602	NM_074119.5 Latest version!	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa345997	NM_001276955.2 Latest version!	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa529898	NM_001356755.2 Latest version!	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa851011	NM_001373156.1 Latest version!	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa345602	NM_074119.4	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa529898	NM_001356755.1	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa345997	NM_001276955.1	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nep-21 ( NM_074119.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506520.1 Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Price & Availability↑

CloneID	OCa345602
Clone ID Related Accession (Same CDS sequence)	NM_074119.5 , NM_074119.4
Accession Version	NM_074119.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_074119.4.

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTT TCTCACCATT TTTGCCACCA 
TCTGCACGTG AGCCATTCTA TCAATTCAAA GCAAATCAAA CTGGAATGTT CAATAGTCCA 
CCACCAGACA GATGGGAGGA TTGTGTAACT CTATCAGTAA TTATGATGGA CATGCCAGTT 
GGACGACTGT TTGTGGAAAA CTTTTTCGAA AAGGAAAGAG CTATGAAAAA GATGACCGAG 
CTCACTTCCT ATCTCAAAAA TGAATTCATC AAACAACTTC ACGTACTCGA CTGGATGGAC 
GAGATAACGA GACGTCGTGC AATTTCAAAG GCAAATATGA TCGAGTATAA ATCAGGATTC 
CCAATGGTTT TATTCAATGA CACTTGGATG GAAAAGAATT GGGGAATGAT TATAAAGCCA 
CGCGAATATT TACTTCATCT CACAATTCGT GTAAAATTGG TCAGATTCAC AGAAGAGCTT 
CTCCGTCTTG ATCAACCATT GGATAGAAGC ATGTGGTTCC AGAGTCCTGC TCAAGTTGAT 
GCATATTATG CTCCAAATAA TAATGAAATG ATCTTCCCCG CGGGCATCAT GCAATTCCCG 
TTTCTAACTC TTGGAGTTCC AAACTACATC ACTTACGGAA TGGTTGGAGC AGTCATTGGA 
CATGAAGTAT CTCATGCATT TGATGATCAG GGAGGACAAT ACGATGAAAT GGGGAATTTG 
AATGATTGGT GGGATGCTGA AACAGAGGAA AAGTTCATCG AGAAAACAAG ATGCTTTGTA 
AGGCAATATG AGAATGTGCA CGTAGTTGAG GCAGATATTC ATTTGAACGG TCAACTAAGT 
CTTGGAGAGA ATATTGCTGA TAATGGAGGA GTCAAGACAG CTTTTAACGC GTACAAGGCA 
TGGAAATCAA ATACAACAGG AATCAGTGAA CCTGCTCTAC CAGGATTCCA GAACTTCACC 
AGTCAACAGA TGTTCTTCTT GGCTTATGCA AATAACTGGT GTTCATTGGT TCGTCCAAAA 
CATTACATTC AAATAATTCT AACAGACGTG CATGCTCCAT CAAAATATCG TGCAATGATT 
CCACTTCAAA ACCGTCCAGA ATTCGCGAAA GCGTTCCAAT GTCCTATTGG ATCCCCGATG 
AATCCAGAGA GAAAATGCCA AGTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506520.1
CDS	237..2546
Translation	MKPENGAATWHPAKRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_074119.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_074119.5

ORF Insert Sequence:

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTT TCTCACCATT TTTGCCACCA 
TCTGCACGTG AGCCATTCTA TCAATTCAAA GCAAATCAAA CTGGAATGTT CAATAGTCCA 
CCACCAGACA GATGGGAGGA TTGTGTAACT CTATCAGTAA TTATGATGGA CATGCCAGTT 
GGACGACTGT TTGTGGAAAA CTTTTTCGAA AAGGAAAGAG CTATGAAAAA GATGACCGAG 
CTCACTTCCT ATCTCAAAAA TGAATTCATC AAACAACTTC ACGTACTCGA CTGGATGGAC 
GAGATAACGA GACGTCGTGC AATTTCAAAG GCAAATATGA TCGAGTATAA ATCAGGATTC 
CCAATGGTTT TATTCAATGA CACTTGGATG GAAAAGAATT GGGGAATGAT TATAAAGCCA 
CGCGAATATT TACTTCATCT CACAATTCGT GTAAAATTGG TCAGATTCAC AGAAGAGCTT 
CTCCGTCTTG ATCAACCATT GGATAGAAGC ATGTGGTTCC AGAGTCCTGC TCAAGTTGAT 
GCATATTATG CTCCAAATAA TAATGAAATG ATCTTCCCCG CGGGCATCAT GCAATTCCCG 
TTTCTAACTC TTGGAGTTCC AAACTACATC ACTTACGGAA TGGTTGGAGC AGTCATTGGA 
CATGAAGTAT CTCATGCATT TGATGATCAG GGAGGACAAT ACGATGAAAT GGGGAATTTG 
AATGATTGGT GGGATGCTGA AACAGAGGAA AAGTTCATCG AGAAAACAAG ATGCTTTGTA 
AGGCAATATG AGAATGTGCA CGTAGTTGAG GCAGATATTC ATTTGAACGG TCAACTAAGT 
CTTGGAGAGA ATATTGCTGA TAATGGAGGA GTCAAGACAG CTTTTAACGC GTACAAGGCA 
TGGAAATCAA ATACAACAGG AATCAGTGAA CCTGCTCTAC CAGGATTCCA GAACTTCACC 
AGTCAACAGA TGTTCTTCTT GGCTTATGCA AATAACTGGT GTTCATTGGT TCGTCCAAAA 
CATTACATTC AAATAATTCT AACAGACGTG CATGCTCCAT CAAAATATCG TGCAATGATT 
CCACTTCAAA ACCGTCCAGA ATTCGCGAAA GCGTTCCAAT GTCCTATTGG ATCCCCGATG 
AATCCAGAGA GAAAATGCCA AGTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_074119.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_506520.1 Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Price & Availability↑

CloneID	OCa345602
Clone ID Related Accession (Same CDS sequence)	NM_074119.5 , NM_074119.4
Accession Version	NM_074119.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_074119.3. COMPLETENESS: incomplete on both ends.

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTT TCTCACCATT TTTGCCACCA 
TCTGCACGTG AGCCATTCTA TCAATTCAAA GCAAATCAAA CTGGAATGTT CAATAGTCCA 
CCACCAGACA GATGGGAGGA TTGTGTAACT CTATCAGTAA TTATGATGGA CATGCCAGTT 
GGACGACTGT TTGTGGAAAA CTTTTTCGAA AAGGAAAGAG CTATGAAAAA GATGACCGAG 
CTCACTTCCT ATCTCAAAAA TGAATTCATC AAACAACTTC ACGTACTCGA CTGGATGGAC 
GAGATAACGA GACGTCGTGC AATTTCAAAG GCAAATATGA TCGAGTATAA ATCAGGATTC 
CCAATGGTTT TATTCAATGA CACTTGGATG GAAAAGAATT GGGGAATGAT TATAAAGCCA 
CGCGAATATT TACTTCATCT CACAATTCGT GTAAAATTGG TCAGATTCAC AGAAGAGCTT 
CTCCGTCTTG ATCAACCATT GGATAGAAGC ATGTGGTTCC AGAGTCCTGC TCAAGTTGAT 
GCATATTATG CTCCAAATAA TAATGAAATG ATCTTCCCCG CGGGCATCAT GCAATTCCCG 
TTTCTAACTC TTGGAGTTCC AAACTACATC ACTTACGGAA TGGTTGGAGC AGTCATTGGA 
CATGAAGTAT CTCATGCATT TGATGATCAG GGAGGACAAT ACGATGAAAT GGGGAATTTG 
AATGATTGGT GGGATGCTGA AACAGAGGAA AAGTTCATCG AGAAAACAAG ATGCTTTGTA 
AGGCAATATG AGAATGTGCA CGTAGTTGAG GCAGATATTC ATTTGAACGG TCAACTAAGT 
CTTGGAGAGA ATATTGCTGA TAATGGAGGA GTCAAGACAG CTTTTAACGC GTACAAGGCA 
TGGAAATCAA ATACAACAGG AATCAGTGAA CCTGCTCTAC CAGGATTCCA GAACTTCACC 
AGTCAACAGA TGTTCTTCTT GGCTTATGCA AATAACTGGT GTTCATTGGT TCGTCCAAAA 
CATTACATTC AAATAATTCT AACAGACGTG CATGCTCCAT CAAAATATCG TGCAATGATT 
CCACTTCAAA ACCGTCCAGA ATTCGCGAAA GCGTTCCAAT GTCCTATTGG ATCCCCGATG 
AATCCAGAGA GAAAATGCCA AGTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_506520.1
CDS	1..2310
Translation	MKPENGAATWHPAKRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_074119.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_074119.4

ORF Insert Sequence:

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTT TCTCACCATT TTTGCCACCA 
TCTGCACGTG AGCCATTCTA TCAATTCAAA GCAAATCAAA CTGGAATGTT CAATAGTCCA 
CCACCAGACA GATGGGAGGA TTGTGTAACT CTATCAGTAA TTATGATGGA CATGCCAGTT 
GGACGACTGT TTGTGGAAAA CTTTTTCGAA AAGGAAAGAG CTATGAAAAA GATGACCGAG 
CTCACTTCCT ATCTCAAAAA TGAATTCATC AAACAACTTC ACGTACTCGA CTGGATGGAC 
GAGATAACGA GACGTCGTGC AATTTCAAAG GCAAATATGA TCGAGTATAA ATCAGGATTC 
CCAATGGTTT TATTCAATGA CACTTGGATG GAAAAGAATT GGGGAATGAT TATAAAGCCA 
CGCGAATATT TACTTCATCT CACAATTCGT GTAAAATTGG TCAGATTCAC AGAAGAGCTT 
CTCCGTCTTG ATCAACCATT GGATAGAAGC ATGTGGTTCC AGAGTCCTGC TCAAGTTGAT 
GCATATTATG CTCCAAATAA TAATGAAATG ATCTTCCCCG CGGGCATCAT GCAATTCCCG 
TTTCTAACTC TTGGAGTTCC AAACTACATC ACTTACGGAA TGGTTGGAGC AGTCATTGGA 
CATGAAGTAT CTCATGCATT TGATGATCAG GGAGGACAAT ACGATGAAAT GGGGAATTTG 
AATGATTGGT GGGATGCTGA AACAGAGGAA AAGTTCATCG AGAAAACAAG ATGCTTTGTA 
AGGCAATATG AGAATGTGCA CGTAGTTGAG GCAGATATTC ATTTGAACGG TCAACTAAGT 
CTTGGAGAGA ATATTGCTGA TAATGGAGGA GTCAAGACAG CTTTTAACGC GTACAAGGCA 
TGGAAATCAA ATACAACAGG AATCAGTGAA CCTGCTCTAC CAGGATTCCA GAACTTCACC 
AGTCAACAGA TGTTCTTCTT GGCTTATGCA AATAACTGGT GTTCATTGGT TCGTCCAAAA 
CATTACATTC AAATAATTCT AACAGACGTG CATGCTCCAT CAAAATATCG TGCAATGATT 
CCACTTCAAA ACCGTCCAGA ATTCGCGAAA GCGTTCCAAT GTCCTATTGG ATCCCCGATG 
AATCCAGAGA GAAAATGCCA AGTTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_001276955.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001263884.1 Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Price & Availability↑

CloneID	OCa345997
Clone ID Related Accession (Same CDS sequence)	NM_001276955.1 , NM_001276955.2
Accession Version	NM_001276955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTG GGCCGACCGG AGACTATTTC 
AGTTTCTCAC CATTTTTGCC ACCATCTGCA CGTGAGCCAT TCTATCAATT CAAAGCAAAT 
CAAACTGGAA TGTTCAATAG TCCACCACCA GACAGATGGG AGGATTGTGT AACTCTATCA 
GTAATTATGA TGGACATGCC AGTTGGACGA CTGTTTGTGG AAAACTTTTT CGAAAAGGAA 
AGAGCTATGA AAAAGATGAC CGAGCTCACT TCCTATCTCA AAAATGAATT CATCAAACAA 
CTTCACGTAC TCGACTGGAT GGACGAGATA ACGAGACGTC GTGCAATTTC AAAGGCAAAT 
ATGATCGAGT ATAAATCAGG ATTCCCAATG GTTTTATTCA ATGACACTTG GATGGAAAAG 
AATTGGGGAA TGATTATAAA GCCACGCGAA TATTTACTTC ATCTCACAAT TCGTGTAAAA 
TTGGTCAGAT TCACAGAAGA GCTTCTCCGT CTTGATCAAC CATTGGATAG AAGCATGTGG 
TTCCAGAGTC CTGCTCAAGT TGATGCATAT TATGCTCCAA ATAATAATGA AATGATCTTC 
CCCGCGGGCA TCATGCAATT CCCGTTTCTA ACTCTTGGAG TTCCAAACTA CATCACTTAC 
GGAATGGTTG GAGCAGTCAT TGGACATGAA GTATCTCATG CATTTGATGA TCAGGGAGGA 
CAATACGATG AAATGGGGAA TTTGAATGAT TGGTGGGATG CTGAAACAGA GGAAAAGTTC 
ATCGAGAAAA CAAGATGCTT TGTAAGGCAA TATGAGAATG TGCACGTAGT TGAGGCAGAT 
ATTCATTTGA ACGGTCAACT AAGTCTTGGA GAGAATATTG CTGATAATGG AGGAGTCAAG 
ACAGCTTTTA ACGCGTACAA GGCATGGAAA TCAAATACAA CAGGAATCAG TGAACCTGCT 
CTACCAGGAT TCCAGAACTT CACCAGTCAA CAGATGTTCT TCTTGGCTTA TGCAAATAAC 
TGGTGTTCAT TGGTTCGTCC AAAACATTAC ATTCAAATAA TTCTAACAGA CGTGCATGCT 
CCATCAAAAT ATCGTGCAAT GATTCCACTT CAAAACCGTC CAGAATTCGC GAAAGCGTTC 
CAATGTCCTA TTGGATCCCC GATGAATCCA GAGAGAAAAT GCCAAGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263884.1
CDS	1..2334
Translation	MKPENGAATWHPAKRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGGPTGDYFSFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_001276955.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276955.1

ORF Insert Sequence:

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTG GGCCGACCGG AGACTATTTC 
AGTTTCTCAC CATTTTTGCC ACCATCTGCA CGTGAGCCAT TCTATCAATT CAAAGCAAAT 
CAAACTGGAA TGTTCAATAG TCCACCACCA GACAGATGGG AGGATTGTGT AACTCTATCA 
GTAATTATGA TGGACATGCC AGTTGGACGA CTGTTTGTGG AAAACTTTTT CGAAAAGGAA 
AGAGCTATGA AAAAGATGAC CGAGCTCACT TCCTATCTCA AAAATGAATT CATCAAACAA 
CTTCACGTAC TCGACTGGAT GGACGAGATA ACGAGACGTC GTGCAATTTC AAAGGCAAAT 
ATGATCGAGT ATAAATCAGG ATTCCCAATG GTTTTATTCA ATGACACTTG GATGGAAAAG 
AATTGGGGAA TGATTATAAA GCCACGCGAA TATTTACTTC ATCTCACAAT TCGTGTAAAA 
TTGGTCAGAT TCACAGAAGA GCTTCTCCGT CTTGATCAAC CATTGGATAG AAGCATGTGG 
TTCCAGAGTC CTGCTCAAGT TGATGCATAT TATGCTCCAA ATAATAATGA AATGATCTTC 
CCCGCGGGCA TCATGCAATT CCCGTTTCTA ACTCTTGGAG TTCCAAACTA CATCACTTAC 
GGAATGGTTG GAGCAGTCAT TGGACATGAA GTATCTCATG CATTTGATGA TCAGGGAGGA 
CAATACGATG AAATGGGGAA TTTGAATGAT TGGTGGGATG CTGAAACAGA GGAAAAGTTC 
ATCGAGAAAA CAAGATGCTT TGTAAGGCAA TATGAGAATG TGCACGTAGT TGAGGCAGAT 
ATTCATTTGA ACGGTCAACT AAGTCTTGGA GAGAATATTG CTGATAATGG AGGAGTCAAG 
ACAGCTTTTA ACGCGTACAA GGCATGGAAA TCAAATACAA CAGGAATCAG TGAACCTGCT 
CTACCAGGAT TCCAGAACTT CACCAGTCAA CAGATGTTCT TCTTGGCTTA TGCAAATAAC 
TGGTGTTCAT TGGTTCGTCC AAAACATTAC ATTCAAATAA TTCTAACAGA CGTGCATGCT 
CCATCAAAAT ATCGTGCAAT GATTCCACTT CAAAACCGTC CAGAATTCGC GAAAGCGTTC 
CAATGTCCTA TTGGATCCCC GATGAATCCA GAGAGAAAAT GCCAAGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_001276955.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001263884.1 Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

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CloneID	OCa345997
Clone ID Related Accession (Same CDS sequence)	NM_001276955.1 , NM_001276955.2
Accession Version	NM_001276955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001276955.1.

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTG GGCCGACCGG AGACTATTTC 
AGTTTCTCAC CATTTTTGCC ACCATCTGCA CGTGAGCCAT TCTATCAATT CAAAGCAAAT 
CAAACTGGAA TGTTCAATAG TCCACCACCA GACAGATGGG AGGATTGTGT AACTCTATCA 
GTAATTATGA TGGACATGCC AGTTGGACGA CTGTTTGTGG AAAACTTTTT CGAAAAGGAA 
AGAGCTATGA AAAAGATGAC CGAGCTCACT TCCTATCTCA AAAATGAATT CATCAAACAA 
CTTCACGTAC TCGACTGGAT GGACGAGATA ACGAGACGTC GTGCAATTTC AAAGGCAAAT 
ATGATCGAGT ATAAATCAGG ATTCCCAATG GTTTTATTCA ATGACACTTG GATGGAAAAG 
AATTGGGGAA TGATTATAAA GCCACGCGAA TATTTACTTC ATCTCACAAT TCGTGTAAAA 
TTGGTCAGAT TCACAGAAGA GCTTCTCCGT CTTGATCAAC CATTGGATAG AAGCATGTGG 
TTCCAGAGTC CTGCTCAAGT TGATGCATAT TATGCTCCAA ATAATAATGA AATGATCTTC 
CCCGCGGGCA TCATGCAATT CCCGTTTCTA ACTCTTGGAG TTCCAAACTA CATCACTTAC 
GGAATGGTTG GAGCAGTCAT TGGACATGAA GTATCTCATG CATTTGATGA TCAGGGAGGA 
CAATACGATG AAATGGGGAA TTTGAATGAT TGGTGGGATG CTGAAACAGA GGAAAAGTTC 
ATCGAGAAAA CAAGATGCTT TGTAAGGCAA TATGAGAATG TGCACGTAGT TGAGGCAGAT 
ATTCATTTGA ACGGTCAACT AAGTCTTGGA GAGAATATTG CTGATAATGG AGGAGTCAAG 
ACAGCTTTTA ACGCGTACAA GGCATGGAAA TCAAATACAA CAGGAATCAG TGAACCTGCT 
CTACCAGGAT TCCAGAACTT CACCAGTCAA CAGATGTTCT TCTTGGCTTA TGCAAATAAC 
TGGTGTTCAT TGGTTCGTCC AAAACATTAC ATTCAAATAA TTCTAACAGA CGTGCATGCT 
CCATCAAAAT ATCGTGCAAT GATTCCACTT CAAAACCGTC CAGAATTCGC GAAAGCGTTC 
CAATGTCCTA TTGGATCCCC GATGAATCCA GAGAGAAAAT GCCAAGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263884.1
CDS	237..2570
Translation	MKPENGAATWHPAKRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGGPTGDYFSFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_001276955.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276955.2

ORF Insert Sequence:

ATGAAGCCCG AGAATGGTGC AGCGACTTGG CACCCGGCCA AAAGGAGTTG CTTGGGCCGG 
TTAACAACGC TGGAAACGTT GCTTCTAGTA TTTCTTGGTC TCCTTATCAC CGCTCTTCTT 
TCTGTGTTAT TTTTATGGTT ATGGGTACTT GACGGATACA AAACGTTCAC TGATGGTCGA 
CCGATATATC CGTTACCGTT TGAAAATTCG TCAGTTGCAG TCGATCGATC AGCAAAGAAT 
CATAATGACG TGGTATGCAC GTCACGAGAG TGTGTACGGC TCGCAGGTTT TTTGGCTGAA 
AATTTGAATT CAAAGATAAA TCCGTGTGAA GATTTTTACG AATTCGCTTG CGGTAACTAC 
GGACTGAACA AAAATCTCCC AGCCAACAAG CCACTTCGTC ATACAATTAG CGATGTGCAA 
TCACGACTGA ACAAACAGGT CAAATCAATG CTTCAATCGC CGATTTCCGC AAATGAAAAA 
CCGTGGGATA AAGTTGCAAA AGGATATTAT CAAAAGTGTT TGGATGAGGA AGAATTAGAA 
TCCACTGGAG TTGAGGCAAT GCGGGATATT GCAAAGAGGA TTGGAGGATG GCCAACTCTA 
GAAGGAGACA AATGGCAAGA ATGGTCCCAT TCTTGGGAAG AACAAATTGC TCTGGTACTA 
AACCTGACTG GGGTGAATGC AGTGATTCTT GAGATGGCTG TGACCCATGA TCCATCAAAT 
AGCAGTCGAA GTGTTATTGA ATTGGATCAA CCAAAATGGG GTGCAGGTTC CCGTTATCCA 
TACCTAAGTG GTGCCAATGA TCCGATGCTG CGAAATTATA CAACTCTCAT GAAAATGACT 
GCAGTCGCAT TGGGAGCTGA TCCAGCAATT GCCGAAAAAG AAATGAATGA GGCTATGGAA 
TTTGAATTGA AGCTAGTGAA CTTCTCGGCA GATGATATGG TTCGAAGAGA TCCTGAGAGA 
GGAAATAATC GATTCGAATT GTGGCAATTG AAAAGTGTTT TTCCATTTAT AAACTTCGAA 
AAGTACCTTA AAACCGTATT CAAGGAGTTG GTGGCGTTGT CTCCAAACCA TACAGTGATT 
GTCAGAGAGA TCGATTATTT TGTGGGAATT CAGCACGTGC TCCAATCAAC TCCAAAACGT 
GTTCTGGCCA ACTACATCTC ATGGCGTTTG GTCCAAGGTG GGCCGACCGG AGACTATTTC 
AGTTTCTCAC CATTTTTGCC ACCATCTGCA CGTGAGCCAT TCTATCAATT CAAAGCAAAT 
CAAACTGGAA TGTTCAATAG TCCACCACCA GACAGATGGG AGGATTGTGT AACTCTATCA 
GTAATTATGA TGGACATGCC AGTTGGACGA CTGTTTGTGG AAAACTTTTT CGAAAAGGAA 
AGAGCTATGA AAAAGATGAC CGAGCTCACT TCCTATCTCA AAAATGAATT CATCAAACAA 
CTTCACGTAC TCGACTGGAT GGACGAGATA ACGAGACGTC GTGCAATTTC AAAGGCAAAT 
ATGATCGAGT ATAAATCAGG ATTCCCAATG GTTTTATTCA ATGACACTTG GATGGAAAAG 
AATTGGGGAA TGATTATAAA GCCACGCGAA TATTTACTTC ATCTCACAAT TCGTGTAAAA 
TTGGTCAGAT TCACAGAAGA GCTTCTCCGT CTTGATCAAC CATTGGATAG AAGCATGTGG 
TTCCAGAGTC CTGCTCAAGT TGATGCATAT TATGCTCCAA ATAATAATGA AATGATCTTC 
CCCGCGGGCA TCATGCAATT CCCGTTTCTA ACTCTTGGAG TTCCAAACTA CATCACTTAC 
GGAATGGTTG GAGCAGTCAT TGGACATGAA GTATCTCATG CATTTGATGA TCAGGGAGGA 
CAATACGATG AAATGGGGAA TTTGAATGAT TGGTGGGATG CTGAAACAGA GGAAAAGTTC 
ATCGAGAAAA CAAGATGCTT TGTAAGGCAA TATGAGAATG TGCACGTAGT TGAGGCAGAT 
ATTCATTTGA ACGGTCAACT AAGTCTTGGA GAGAATATTG CTGATAATGG AGGAGTCAAG 
ACAGCTTTTA ACGCGTACAA GGCATGGAAA TCAAATACAA CAGGAATCAG TGAACCTGCT 
CTACCAGGAT TCCAGAACTT CACCAGTCAA CAGATGTTCT TCTTGGCTTA TGCAAATAAC 
TGGTGTTCAT TGGTTCGTCC AAAACATTAC ATTCAAATAA TTCTAACAGA CGTGCATGCT 
CCATCAAAAT ATCGTGCAAT GATTCCACTT CAAAACCGTC CAGAATTCGC GAAAGCGTTC 
CAATGTCCTA TTGGATCCCC GATGAATCCA GAGAGAAAAT GCCAAGTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_001356755.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343820.1 Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

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CloneID	OCa529898
Clone ID Related Accession (Same CDS sequence)	NM_001356755.2 , NM_001356755.1
Accession Version	NM_001356755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001356755.1.

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
TTCTCACCAT TTTTGCCACC ATCTGCACGT GAGCCATTCT ATCAATTCAA AGCAAATCAA 
ACTGGAATGT TCAATAGTCC ACCACCAGAC AGATGGGAGG ATTGTGTAAC TCTATCAGTA 
ATTATGATGG ACATGCCAGT TGGACGACTG TTTGTGGAAA ACTTTTTCGA AAAGGAAAGA 
GCTATGAAAA AGATGACCGA GCTCACTTCC TATCTCAAAA ATGAATTCAT CAAACAACTT 
CACGTACTCG ACTGGATGGA CGAGATAACG AGACGTCGTG CAATTTCAAA GGCAAATATG 
ATCGAGTATA AATCAGGATT CCCAATGGTT TTATTCAATG ACACTTGGAT GGAAAAGAAT 
TGGGGAATGA TTATAAAGCC ACGCGAATAT TTACTTCATC TCACAATTCG TGTAAAATTG 
GTCAGATTCA CAGAAGAGCT TCTCCGTCTT GATCAACCAT TGGATAGAAG CATGTGGTTC 
CAGAGTCCTG CTCAAGTTGA TGCATATTAT GCTCCAAATA ATAATGAAAT GATCTTCCCC 
GCGGGCATCA TGCAATTCCC GTTTCTAACT CTTGGAGTTC CAAACTACAT CACTTACGGA 
ATGGTTGGAG CAGTCATTGG ACATGAAGTA TCTCATGCAT TTGATGATCA GGGAGGACAA 
TACGATGAAA TGGGGAATTT GAATGATTGG TGGGATGCTG AAACAGAGGA AAAGTTCATC 
GAGAAAACAA GATGCTTTGT AAGGCAATAT GAGAATGTGC ACGTAGTTGA GGCAGATATT 
CATTTGAACG GTCAACTAAG TCTTGGAGAG AATATTGCTG ATAATGGAGG AGTCAAGACA 
GCTTTTAACG CGTACAAGGC ATGGAAATCA AATACAACAG GAATCAGTGA ACCTGCTCTA 
CCAGGATTCC AGAACTTCAC CAGTCAACAG ATGTTCTTCT TGGCTTATGC AAATAACTGG 
TGTTCATTGG TTCGTCCAAA ACATTACATT CAAATAATTC TAACAGACGT GCATGCTCCA 
TCAAAATATC GTGCAATGAT TCCACTTCAA AACCGTCCAG AATTCGCGAA AGCGTTCCAA 
TGTCCTATTG GATCCCCGAT GAATCCAGAG AGAAAATGCC AAGTTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343820.1
CDS	56..2626
Translation	MNLNPHLASTSSSTTSSTSSTEFPRVTVNSRRAHLPHHLSSNGIQQPHIYACSDENSYYASPIFLTRTIDEPPKKPAPTLSRQSTCRPTSQLYLPSKKRKTRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_001356755.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356755.2

ORF Insert Sequence:

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
TTCTCACCAT TTTTGCCACC ATCTGCACGT GAGCCATTCT ATCAATTCAA AGCAAATCAA 
ACTGGAATGT TCAATAGTCC ACCACCAGAC AGATGGGAGG ATTGTGTAAC TCTATCAGTA 
ATTATGATGG ACATGCCAGT TGGACGACTG TTTGTGGAAA ACTTTTTCGA AAAGGAAAGA 
GCTATGAAAA AGATGACCGA GCTCACTTCC TATCTCAAAA ATGAATTCAT CAAACAACTT 
CACGTACTCG ACTGGATGGA CGAGATAACG AGACGTCGTG CAATTTCAAA GGCAAATATG 
ATCGAGTATA AATCAGGATT CCCAATGGTT TTATTCAATG ACACTTGGAT GGAAAAGAAT 
TGGGGAATGA TTATAAAGCC ACGCGAATAT TTACTTCATC TCACAATTCG TGTAAAATTG 
GTCAGATTCA CAGAAGAGCT TCTCCGTCTT GATCAACCAT TGGATAGAAG CATGTGGTTC 
CAGAGTCCTG CTCAAGTTGA TGCATATTAT GCTCCAAATA ATAATGAAAT GATCTTCCCC 
GCGGGCATCA TGCAATTCCC GTTTCTAACT CTTGGAGTTC CAAACTACAT CACTTACGGA 
ATGGTTGGAG CAGTCATTGG ACATGAAGTA TCTCATGCAT TTGATGATCA GGGAGGACAA 
TACGATGAAA TGGGGAATTT GAATGATTGG TGGGATGCTG AAACAGAGGA AAAGTTCATC 
GAGAAAACAA GATGCTTTGT AAGGCAATAT GAGAATGTGC ACGTAGTTGA GGCAGATATT 
CATTTGAACG GTCAACTAAG TCTTGGAGAG AATATTGCTG ATAATGGAGG AGTCAAGACA 
GCTTTTAACG CGTACAAGGC ATGGAAATCA AATACAACAG GAATCAGTGA ACCTGCTCTA 
CCAGGATTCC AGAACTTCAC CAGTCAACAG ATGTTCTTCT TGGCTTATGC AAATAACTGG 
TGTTCATTGG TTCGTCCAAA ACATTACATT CAAATAATTC TAACAGACGT GCATGCTCCA 
TCAAAATATC GTGCAATGAT TCCACTTCAA AACCGTCCAG AATTCGCGAA AGCGTTCCAA 
TGTCCTATTG GATCCCCGAT GAATCCAGAG AGAAAATGCC AAGTTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_001356755.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001343820.1 Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Price & Availability↑

CloneID	OCa529898
Clone ID Related Accession (Same CDS sequence)	NM_001356755.2 , NM_001356755.1
Accession Version	NM_001356755.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
TTCTCACCAT TTTTGCCACC ATCTGCACGT GAGCCATTCT ATCAATTCAA AGCAAATCAA 
ACTGGAATGT TCAATAGTCC ACCACCAGAC AGATGGGAGG ATTGTGTAAC TCTATCAGTA 
ATTATGATGG ACATGCCAGT TGGACGACTG TTTGTGGAAA ACTTTTTCGA AAAGGAAAGA 
GCTATGAAAA AGATGACCGA GCTCACTTCC TATCTCAAAA ATGAATTCAT CAAACAACTT 
CACGTACTCG ACTGGATGGA CGAGATAACG AGACGTCGTG CAATTTCAAA GGCAAATATG 
ATCGAGTATA AATCAGGATT CCCAATGGTT TTATTCAATG ACACTTGGAT GGAAAAGAAT 
TGGGGAATGA TTATAAAGCC ACGCGAATAT TTACTTCATC TCACAATTCG TGTAAAATTG 
GTCAGATTCA CAGAAGAGCT TCTCCGTCTT GATCAACCAT TGGATAGAAG CATGTGGTTC 
CAGAGTCCTG CTCAAGTTGA TGCATATTAT GCTCCAAATA ATAATGAAAT GATCTTCCCC 
GCGGGCATCA TGCAATTCCC GTTTCTAACT CTTGGAGTTC CAAACTACAT CACTTACGGA 
ATGGTTGGAG CAGTCATTGG ACATGAAGTA TCTCATGCAT TTGATGATCA GGGAGGACAA 
TACGATGAAA TGGGGAATTT GAATGATTGG TGGGATGCTG AAACAGAGGA AAAGTTCATC 
GAGAAAACAA GATGCTTTGT AAGGCAATAT GAGAATGTGC ACGTAGTTGA GGCAGATATT 
CATTTGAACG GTCAACTAAG TCTTGGAGAG AATATTGCTG ATAATGGAGG AGTCAAGACA 
GCTTTTAACG CGTACAAGGC ATGGAAATCA AATACAACAG GAATCAGTGA ACCTGCTCTA 
CCAGGATTCC AGAACTTCAC CAGTCAACAG ATGTTCTTCT TGGCTTATGC AAATAACTGG 
TGTTCATTGG TTCGTCCAAA ACATTACATT CAAATAATTC TAACAGACGT GCATGCTCCA 
TCAAAATATC GTGCAATGAT TCCACTTCAA AACCGTCCAG AATTCGCGAA AGCGTTCCAA 
TGTCCTATTG GATCCCCGAT GAATCCAGAG AGAAAATGCC AAGTTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343820.1
CDS	1..2571
Translation	MNLNPHLASTSSSTTSSTSSTEFPRVTVNSRRAHLPHHLSSNGIQQPHIYACSDENSYYASPIFLTRTIDEPPKKPAPTLSRQSTCRPTSQLYLPSKKRKTRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_001356755.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356755.1

ORF Insert Sequence:

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
TTCTCACCAT TTTTGCCACC ATCTGCACGT GAGCCATTCT ATCAATTCAA AGCAAATCAA 
ACTGGAATGT TCAATAGTCC ACCACCAGAC AGATGGGAGG ATTGTGTAAC TCTATCAGTA 
ATTATGATGG ACATGCCAGT TGGACGACTG TTTGTGGAAA ACTTTTTCGA AAAGGAAAGA 
GCTATGAAAA AGATGACCGA GCTCACTTCC TATCTCAAAA ATGAATTCAT CAAACAACTT 
CACGTACTCG ACTGGATGGA CGAGATAACG AGACGTCGTG CAATTTCAAA GGCAAATATG 
ATCGAGTATA AATCAGGATT CCCAATGGTT TTATTCAATG ACACTTGGAT GGAAAAGAAT 
TGGGGAATGA TTATAAAGCC ACGCGAATAT TTACTTCATC TCACAATTCG TGTAAAATTG 
GTCAGATTCA CAGAAGAGCT TCTCCGTCTT GATCAACCAT TGGATAGAAG CATGTGGTTC 
CAGAGTCCTG CTCAAGTTGA TGCATATTAT GCTCCAAATA ATAATGAAAT GATCTTCCCC 
GCGGGCATCA TGCAATTCCC GTTTCTAACT CTTGGAGTTC CAAACTACAT CACTTACGGA 
ATGGTTGGAG CAGTCATTGG ACATGAAGTA TCTCATGCAT TTGATGATCA GGGAGGACAA 
TACGATGAAA TGGGGAATTT GAATGATTGG TGGGATGCTG AAACAGAGGA AAAGTTCATC 
GAGAAAACAA GATGCTTTGT AAGGCAATAT GAGAATGTGC ACGTAGTTGA GGCAGATATT 
CATTTGAACG GTCAACTAAG TCTTGGAGAG AATATTGCTG ATAATGGAGG AGTCAAGACA 
GCTTTTAACG CGTACAAGGC ATGGAAATCA AATACAACAG GAATCAGTGA ACCTGCTCTA 
CCAGGATTCC AGAACTTCAC CAGTCAACAG ATGTTCTTCT TGGCTTATGC AAATAACTGG 
TGTTCATTGG TTCGTCCAAA ACATTACATT CAAATAATTC TAACAGACGT GCATGCTCCA 
TCAAAATATC GTGCAATGAT TCCACTTCAA AACCGTCCAG AATTCGCGAA AGCGTTCCAA 
TGTCCTATTG GATCCCCGAT GAATCCAGAG AGAAAATGCC AAGTTTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nep-21 ( NM_001373156.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360525.1 Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Price & Availability↑

CloneID	OCa851011
Clone ID Related Accession (Same CDS sequence)	NM_001373156.1
Accession Version	NM_001373156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Neprilysin-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283).

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
GGGCCGACCG GAGACTATTT CAGTTTCTCA CCATTTTTGC CACCATCTGC ACGTGAGCCA 
TTCTATCAAT TCAAAGCAAA TCAAACTGGA ATGTTCAATA GTCCACCACC AGACAGATGG 
GAGGATTGTG TAACTCTATC AGTAATTATG ATGGACATGC CAGTTGGACG ACTGTTTGTG 
GAAAACTTTT TCGAAAAGGA AAGAGCTATG AAAAAGATGA CCGAGCTCAC TTCCTATCTC 
AAAAATGAAT TCATCAAACA ACTTCACGTA CTCGACTGGA TGGACGAGAT AACGAGACGT 
CGTGCAATTT CAAAGGCAAA TATGATCGAG TATAAATCAG GATTCCCAAT GGTTTTATTC 
AATGACACTT GGATGGAAAA GAATTGGGGA ATGATTATAA AGCCACGCGA ATATTTACTT 
CATCTCACAA TTCGTGTAAA ATTGGTCAGA TTCACAGAAG AGCTTCTCCG TCTTGATCAA 
CCATTGGATA GAAGCATGTG GTTCCAGAGT CCTGCTCAAG TTGATGCATA TTATGCTCCA 
AATAATAATG AAATGATCTT CCCCGCGGGC ATCATGCAAT TCCCGTTTCT AACTCTTGGA 
GTTCCAAACT ACATCACTTA CGGAATGGTT GGAGCAGTCA TTGGACATGA AGTATCTCAT 
GCATTTGATG ATCAGGGAGG ACAATACGAT GAAATGGGGA ATTTGAATGA TTGGTGGGAT 
GCTGAAACAG AGGAAAAGTT CATCGAGAAA ACAAGATGCT TTGTAAGGCA ATATGAGAAT 
GTGCACGTAG TTGAGGCAGA TATTCATTTG AACGGTCAAC TAAGTCTTGG AGAGAATATT 
GCTGATAATG GAGGAGTCAA GACAGCTTTT AACGCGTACA AGGCATGGAA ATCAAATACA 
ACAGGAATCA GTGAACCTGC TCTACCAGGA TTCCAGAACT TCACCAGTCA ACAGATGTTC 
TTCTTGGCTT ATGCAAATAA CTGGTGTTCA TTGGTTCGTC CAAAACATTA CATTCAAATA 
ATTCTAACAG ACGTGCATGC TCCATCAAAA TATCGTGCAA TGATTCCACT TCAAAACCGT 
CCAGAATTCG CGAAAGCGTT CCAATGTCCT ATTGGATCCC CGATGAATCC AGAGAGAAAA 
TGCCAAGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360525.1
CDS	60..2654
Translation	MNLNPHLASTSSSTTSSTSSTEFPRVTVNSRRAHLPHHLSSNGIQQPHIYACSDENSYYASPIFLTRTIDEPPKKPAPTLSRQSTCRPTSQLYLPSKKRKTRSCLGRLTTLETLLLVFLGLLITALLSVLFLWLWVLDGYKTFTDGRPIYPLPFENSSVAVDRSAKNHNDVVCTSRECVRLAGFLAENLNSKINPCEDFYEFACGNYGLNKNLPANKPLRHTISDVQSRLNKQVKSMLQSPISANEKPWDKVAKGYYQKCLDEEELESTGVEAMRDIAKRIGGWPTLEGDKWQEWSHSWEEQIALVLNLTGVNAVILEMAVTHDPSNSSRSVIELDQPKWGAGSRYPYLSGANDPMLRNYTTLMKMTAVALGADPAIAEKEMNEAMEFELKLVNFSADDMVRRDPERGNNRFELWQLKSVFPFINFEKYLKTVFKELVALSPNHTVIVREIDYFVGIQHVLQSTPKRVLANYISWRLVQGGPTGDYFSFSPFLPPSAREPFYQFKANQTGMFNSPPPDRWEDCVTLSVIMMDMPVGRLFVENFFEKERAMKKMTELTSYLKNEFIKQLHVLDWMDEITRRRAISKANMIEYKSGFPMVLFNDTWMEKNWGMIIKPREYLLHLTIRVKLVRFTEELLRLDQPLDRSMWFQSPAQVDAYYAPNNNEMIFPAGIMQFPFLTLGVPNYITYGMVGAVIGHEVSHAFDDQGGQYDEMGNLNDWWDAETEEKFIEKTRCFVRQYENVHVVEADIHLNGQLSLGENIADNGGVKTAFNAYKAWKSNTTGISEPALPGFQNFTSQQMFFLAYANNWCSLVRPKHYIQIILTDVHAPSKYRAMIPLQNRPEFAKAFQCPIGSPMNPERKCQVW

Target ORF information:

RefSeq Version	NM_001373156.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Neprilysin-21 (nep-21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373156.1

ORF Insert Sequence:

ATGAATTTGA ACCCCCATCT CGCTTCGACA TCCTCCTCTA CAACATCATC CACATCATCT 
ACCGAGTTTC CACGTGTAAC TGTGAATTCT CGTCGTGCTC ATTTACCTCA TCACTTATCA 
TCAAATGGCA TCCAGCAACC GCACATTTAC GCTTGTTCAG ATGAAAATTC GTATTATGCG 
TCTCCGATCT TCTTGACTCG AACGATAGAC GAACCTCCGA AAAAACCTGC GCCAACGTTA 
AGTCGACAGT CCACGTGTCG GCCGACGAGT CAACTTTACC TACCGAGCAA GAAAAGGAAA 
ACTAGGAGTT GCTTGGGCCG GTTAACAACG CTGGAAACGT TGCTTCTAGT ATTTCTTGGT 
CTCCTTATCA CCGCTCTTCT TTCTGTGTTA TTTTTATGGT TATGGGTACT TGACGGATAC 
AAAACGTTCA CTGATGGTCG ACCGATATAT CCGTTACCGT TTGAAAATTC GTCAGTTGCA 
GTCGATCGAT CAGCAAAGAA TCATAATGAC GTGGTATGCA CGTCACGAGA GTGTGTACGG 
CTCGCAGGTT TTTTGGCTGA AAATTTGAAT TCAAAGATAA ATCCGTGTGA AGATTTTTAC 
GAATTCGCTT GCGGTAACTA CGGACTGAAC AAAAATCTCC CAGCCAACAA GCCACTTCGT 
CATACAATTA GCGATGTGCA ATCACGACTG AACAAACAGG TCAAATCAAT GCTTCAATCG 
CCGATTTCCG CAAATGAAAA ACCGTGGGAT AAAGTTGCAA AAGGATATTA TCAAAAGTGT 
TTGGATGAGG AAGAATTAGA ATCCACTGGA GTTGAGGCAA TGCGGGATAT TGCAAAGAGG 
ATTGGAGGAT GGCCAACTCT AGAAGGAGAC AAATGGCAAG AATGGTCCCA TTCTTGGGAA 
GAACAAATTG CTCTGGTACT AAACCTGACT GGGGTGAATG CAGTGATTCT TGAGATGGCT 
GTGACCCATG ATCCATCAAA TAGCAGTCGA AGTGTTATTG AATTGGATCA ACCAAAATGG 
GGTGCAGGTT CCCGTTATCC ATACCTAAGT GGTGCCAATG ATCCGATGCT GCGAAATTAT 
ACAACTCTCA TGAAAATGAC TGCAGTCGCA TTGGGAGCTG ATCCAGCAAT TGCCGAAAAA 
GAAATGAATG AGGCTATGGA ATTTGAATTG AAGCTAGTGA ACTTCTCGGC AGATGATATG 
GTTCGAAGAG ATCCTGAGAG AGGAAATAAT CGATTCGAAT TGTGGCAATT GAAAAGTGTT 
TTTCCATTTA TAAACTTCGA AAAGTACCTT AAAACCGTAT TCAAGGAGTT GGTGGCGTTG 
TCTCCAAACC ATACAGTGAT TGTCAGAGAG ATCGATTATT TTGTGGGAAT TCAGCACGTG 
CTCCAATCAA CTCCAAAACG TGTTCTGGCC AACTACATCT CATGGCGTTT GGTCCAAGGT 
GGGCCGACCG GAGACTATTT CAGTTTCTCA CCATTTTTGC CACCATCTGC ACGTGAGCCA 
TTCTATCAAT TCAAAGCAAA TCAAACTGGA ATGTTCAATA GTCCACCACC AGACAGATGG 
GAGGATTGTG TAACTCTATC AGTAATTATG ATGGACATGC CAGTTGGACG ACTGTTTGTG 
GAAAACTTTT TCGAAAAGGA AAGAGCTATG AAAAAGATGA CCGAGCTCAC TTCCTATCTC 
AAAAATGAAT TCATCAAACA ACTTCACGTA CTCGACTGGA TGGACGAGAT AACGAGACGT 
CGTGCAATTT CAAAGGCAAA TATGATCGAG TATAAATCAG GATTCCCAAT GGTTTTATTC 
AATGACACTT GGATGGAAAA GAATTGGGGA ATGATTATAA AGCCACGCGA ATATTTACTT 
CATCTCACAA TTCGTGTAAA ATTGGTCAGA TTCACAGAAG AGCTTCTCCG TCTTGATCAA 
CCATTGGATA GAAGCATGTG GTTCCAGAGT CCTGCTCAAG TTGATGCATA TTATGCTCCA 
AATAATAATG AAATGATCTT CCCCGCGGGC ATCATGCAAT TCCCGTTTCT AACTCTTGGA 
GTTCCAAACT ACATCACTTA CGGAATGGTT GGAGCAGTCA TTGGACATGA AGTATCTCAT 
GCATTTGATG ATCAGGGAGG ACAATACGAT GAAATGGGGA ATTTGAATGA TTGGTGGGAT 
GCTGAAACAG AGGAAAAGTT CATCGAGAAA ACAAGATGCT TTGTAAGGCA ATATGAGAAT 
GTGCACGTAG TTGAGGCAGA TATTCATTTG AACGGTCAAC TAAGTCTTGG AGAGAATATT 
GCTGATAATG GAGGAGTCAA GACAGCTTTT AACGCGTACA AGGCATGGAA ATCAAATACA 
ACAGGAATCA GTGAACCTGC TCTACCAGGA TTCCAGAACT TCACCAGTCA ACAGATGTTC 
TTCTTGGCTT ATGCAAATAA CTGGTGTTCA TTGGTTCGTC CAAAACATTA CATTCAAATA 
ATTCTAACAG ACGTGCATGC TCCATCAAAA TATCGTGCAA TGATTCCACT TCAAAACCGT 
CCAGAATTCG CGAAAGCGTT CCAATGTCCT ATTGGATCCC CGATGAATCC AGAGAGAAAA 
TGCCAAGTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nep-21 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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