glp-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	glp-1
Entrez Gene ID	176286
Full Name	Protein glp-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066613.4	NP_499014.1	Protein glp-1
NM_066613.5	NP_499014.1	Protein glp-1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	notch3	NP_571624.2
Caenorhabditis elegans (roundworm)	lin-12	NP_499007.1
Caenorhabditis elegans (roundworm)	glp-1	NP_499014.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zygotically expressed MOM-2 (Wnt) is responsible for two distinct cell-cell interactions as the MS blastomere produces one-third of the body wall muscles. Both the inhibitory and the activating interactions require precise spatiotemporal expression of zygotic MOM-2, which is dependent upon two distinct GLP-1 Notch signals.
Title: Reciprocal signaling by Wnt and Notch specifies a muscle precursor in the C. elegans embryo.

The authors find that Notch-dependent active transcription sites occur in a probabilistic fashion and, unexpectedly, do so in a steep gradient across the stem cell pool.

Molecular and genetic analyses indicate that the loss of ubr-5 function suppresses defects caused by reduced signaling via GLP-1 or LIN-12.
Title: UBR-5, a Conserved HECT-Type E3 Ubiquitin Ligase, Negatively Regulates Notch-Type Signaling in Caenorhabditis elegans.

Study suggests that GLP-1 signaling is directly responsible for driving all stages of proliferation in the wild-type germline, and loss of its activity causes differentiation without intervening, distinctly regulated transit-amplifying divisions.
Title: Analysis of Germline Stem Cell Differentiation Following Loss of GLP-1 Notch Activity in Caenorhabditis elegans.

glp-1(ar202), a temperature-sensitive gain-of-function C. elegans NOTCH mutant, was employed to delineate NOTCH-driven tumor responses to radiotherapy.
Title: Targeting Homologous Recombination in Notch-Driven C. elegans Stem Cell and Human Tumors.

The following glp-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glp-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341791	NM_066613.5 Latest version!	Caenorhabditis elegans Protein glp-1 (glp-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa341791	NM_066613.4	Caenorhabditis elegans Protein glp-1 (glp-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glp-1 ( NM_066613.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499014.1 Caenorhabditis elegans Protein glp-1 (glp-1), partial mRNA.

Price & Availability↑

CloneID	OCa341791
Clone ID Related Accession (Same CDS sequence)	NM_066613.4 , NM_066613.5
Accession Version	NM_066613.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein glp-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066613.3. COMPLETENESS: incomplete on both ends.

ATGCGAGTTC TTCTAATTTT ACTCGCGTTT TTTGCGCCAA TCGCCAGTCA ACTTATGGGT 
GGAGAATGCG GAAGGGAAGG TGCTTGCTCC GTCAATGGAA AATGCTATAA TGGAAAACTG 
ATTGAGACAT ACTGGTGCCG TTGCAAAAAA GGATTCGGAG GTGCTTTCTG TGAACGTGAA 
TGCGATTTGG ATTGTAAACG AGGCGAGAAG TGCATCTACG ATGTTTATGG TGAAAATCCG 
ACGTGTATCT GTCAAGATTG CGAAGACGAG ACTCCTCCAA CAGAACGTAC TCAAAAAGGC 
TGTGAAGAAG GCTATGGAGG TCCTGACTGC AAAACTCCTC TATTTTCGGG AGTAAATCCA 
TGCGATTCGG ATCCTTGCAA CAACGGACTC TGCTATCCAT TCTATGGTGG ATTTCAGTGC 
ATATGCAACA ATGGATATGG AGGATCGTAT TGTGAAGAAG GAATCGATCA TTGTGCTCAA 
AATGAATGCG CAGAAGGTTC AACGTGTGTC AATAGTGTAT ACAACTATTA CTGTGATTGC 
CCAATTGGAA AATCCGGTCG ATATTGTGAA CGAACTGAAT GTGCTTTGAT GGGAAACATT 
TGCAATCATG GAAGATGTAT TCCGAACAGA GATGAAGACA AGAACTTCAG ATGTGTATGC 
GACTCGGGAT ACGAGGGAGA ATTTTGCAAT AAGGATAAAA ACGAATGCCT CATCGAAGAA 
ACGTGTGTTA ACAACTCTAC ATGTTTCAAT TTGCACGGTG ATTTTACTTG TACCTGTAAA 
CCTGGATACG CTGGAAAGTA TTGCGAGGAG GCTATCGACA TGTGCAAGGA TTACGTTTGC 
CAAAATGATG GATACTGTGC CCATGACTCG AATCAGATGC CAATTTGTTA TTGCGAACAA 
GGATTCACTG GACAACGATG TGAGATTGAG TGTCCTTCAG GATTCGGGGG AATTCATTGT 
GATCTTCCAC TACAGAGACC ACACTGCTCT CGGAGCAATG GAACGTGTTA CAACGATGGA 
AGATGTATAA ATGGTTTCTG TGTCTGTGAA CCTGATTATA TTGGAGATCG ATGTGAGATT 
AATAGGAAAG ATTTCAAGTT CCCAGATATT CAGTCATGCA AATACAATCC GTGTGTGAAT 
AATGCGACTT GTATCGATCT CAAAAACAGT GGATATTCAT GTCATTGTCC GCTTGGATTC 
TATGGATTGA ACTGCGAGCA ACATTTGTTG TGCACTCCAA CTACATGTGC TAACGGAGGT 
ACTTGTGAAG GTGTGAATGG CGTTATCAGA TGTAACTGCC CCAACGGATT CTCTGGAGAT 
TACTGCGAAA TAAAGGATAG ACAGTTATGC TCCAGACATC CATGCAAGAA CGGAGGAGTT 
TGTAAGAATA CTGGATATTG CGAATGCCAA TATGGATACA CTGGACCCAC TTGTGAAGAA 
GTTCTAGTTA TCGAGAAATC AAAAGAGACT GTCATTCGTG ATCTATGCGA GCAACGAAAG 
TGTATGGATC TTGCTTCCAA TGGTATTTGC AACCCGGAAT GCAATTTGGA AGAATGTAAT 
TTTGATGGAG GTGATTGCTC AGGAGGGCAA AGACCATTCT CCAAATGTCA ATACCCTGCA 
AGATGTGCTG ATCAATTCGC AAATGGAGTC TGTAATCAGG AATGCAATAA TGAAGAATGT 
CTTTACGATG GATTGGACTG TCAATCAGAG TTATTTCGAT GCCCAGCTCA TATTCGGAAA 
CATTGTATTG AAAGACGAGG CGATGGAGTT TGTAATTTAG AATGCAGTTT CATTGGATGT 
GGTTTTGACG GTGGAGATTG CAATAACGGA ACAGAAGCAA TAATCCTCAG TGATATTCGT 
ATAAAAGTTC AAATTGATCC TATTGAATTC CAAGCTACCG GAGGAGAAAC ACTAATGCAA 
ATCAGTGCAA ATCTTCGAGC AACCGTACGT ATTCAACGGG ATGAACTTGG ACCTCTTGTA 
TTCAGATGGG ATGGTGAACA TGAAATGGAA AGAGTTGAGA TGAATAGTAG CAAGCTCGAA 
GATCAATTTG TACTTTCGCA TCACGTTCGC AGGTATCGGC AAGCTGTTGT CACTGGAATT 
GTCTTGTATT TGGAAGTCGA AGAAATATGT AAACCCGAGT TTTGCCGATT TTCAACAGCG 
CAAAGTGTCG TTGATTTGAT TGCTGCTGGA CTTGTGAAGT CTGATGGTCG TATGTCTCTG 
GGTCTCCCAA TTACGGAAGC AATGGTAGCT GTTCCAAAGA GAAACGAAAT CGATGAAGGA 
TGGTCTCGCA GTCAAGTTAT TCTGTTTGCA TGTATTGCAT TTTTGGCTTT TGGAACTGTT 
GTCGCTGGTG TTATCGCAAA GAATGGTCCA GAAAGAAGTA GAAAAAGAAA AATGGTGAAC 
GCAACAGTCT GGATGCCTCC GATGGAATCA ACGAACGAGA AAGGTCGTCG GAATCAGTCA 
AATCATTCGA GTCAGTGCTC TCTTCTTGAC AATTCTGCCT ACTATCACCC CAATACCAAG 
AGACACTGTA GCGATTATAG TACCGGCTAC AATGGAGAAC AATATTCACA GATTTATCCA 
CAAACTCTCG CAAATGGATA TCCCGGTGAT TACAACGAAT TGAACTTTGA CTTTCAATCT 
GAGACGTTCG CACCAGCTGA TCTACCAGCC GACGAAATCC CTCTCCATGT TCAAGCTGCA 
GGACCAGATG CGATTACAGC TCCAATCACA AATGAGTCAG TCAATCAAGT GGATTCAAAG 
TATAGACGAC GAGTTCTGCA CTGGCTTGCT GCCAATGTCA GAGGAAAACC GGAAGATGTC 
ATTACAACAG AGGCAATTCG ATGCTTGAAG GCTGGAGCCG ATGTGAATGC AAGAGATTGT 
GATGAGAATA CTGCTCTGAT GCTAGCTGTT CGTGCACACC GTGTCAGACT TTCCGTAGTG 
CTTCTTCGTG AAGGAGCTAA TCCAACAATA TTCAATAATT CGGAGCGAAG TGCCCTTCAT 
GAGGCTGTTG TCAACAAGGA TCTGCGTATT CTGCGGCATC TTCTCACTGA TAAACGTCTT 
CTCAAAGAAA TTGATGAATT GGACCGGAAT GGTATGACTG CATTGATGCT AGTAGCACGT 
GAACTCGGAA AACATCAAGT GGAGATGGCA GAGCTTCTTC TTTCAAAAGG AGCAAAACTT 
GATTACGATG GTGCTGCTAG AAAAGATTCC AACAAGTATA AAGGACGAAC AGCTCTTCAT 
TACGCAGCGA TGCACGATAA CGAGGAAATG GTAATAATGC TGGTCCGACG AAGCTCTAAT 
AAGGACAAAC AGGACGAAGA CGGAAGAACA CCGATAATGC TTGCCGCTAA GGAAGGATGT 
GAAAAGACGG TGCAATATTT GGCTCTTAAT GATGCTAGTT TGGGTATAGT CGATTCAATG 
GACATGACTG CTGCTCAAGT TGCTGAAGCC AGCTACCATC ATGAACTTGC GGCTTTTCTT 
CGCCAAGTGG CAAACGAACG GCATCGTAAT GATATCATGC GACAGCAAAT AGTCAAGTCA 
GGTCATGGTG CTAAGTCTGG GCGACAAACT GTGAAGAATA TCAAAAGAGC AGGATCTCGA 
AAAACGCCAA CAAGTGCAGC ATCGTCTCGC GAAACAAATC ATCTGACACC ACCACCGTCT 
GATGGATCGT TCTCATCTCC GTCTCCACAT TATTATCCGA CGACTACATC GACACCGAAT 
CGAATGGAAA CAAGTCCGGA GTACATGTTT AACCATGAAA TGGCACCACC GGTCAATGCG 
ATGTGGTATA CTACACCACC TCCTTATCAA GATCCAAACT ATCGTCATGT GCCTCCAAAT 
ACTGCATTTC AAAATGCAGA GCAAATGAAT GGCTCCTTCT ACTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499014.1
CDS	1..3888
Translation	MRVLLILLAFFAPIASQLMGGECGREGACSVNGKCYNGKLIETYWCRCKKGFGGAFCERECDLDCKRGEKCIYDVYGENPTCICQDCEDETPPTERTQKGCEEGYGGPDCKTPLFSGVNPCDSDPCNNGLCYPFYGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGFCVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCEQHLLCTPTTCANGGTCEGVNGVIRCNCPNGFSGDYCEIKDRQLCSRHPCKNGGVCKNTGYCECQYGYTGPTCEEVLVIEKSKETVIRDLCEQRKCMDLASNGICNPECNLEECNFDGGDCSGGQRPFSKCQYPARCADQFANGVCNQECNNEECLYDGLDCQSELFRCPAHIRKHCIERRGDGVCNLECSFIGCGFDGGDCNNGTEAIILSDIRIKVQIDPIEFQATGGETLMQISANLRATVRIQRDELGPLVFRWDGEHEMERVEMNSSKLEDQFVLSHHVRRYRQAVVTGIVLYLEVEEICKPEFCRFSTAQSVVDLIAAGLVKSDGRMSLGLPITEAMVAVPKRNEIDEGWSRSQVILFACIAFLAFGTVVAGVIAKNGPERSRKRKMVNATVWMPPMESTNEKGRRNQSNHSSQCSLLDNSAYYHPNTKRHCSDYSTGYNGEQYSQIYPQTLANGYPGDYNELNFDFQSETFAPADLPADEIPLHVQAAGPDAITAPITNESVNQVDSKYRRRVLHWLAANVRGKPEDVITTEAIRCLKAGADVNARDCDENTALMLAVRAHRVRLSVVLLREGANPTIFNNSERSALHEAVVNKDLRILRHLLTDKRLLKEIDELDRNGMTALMLVARELGKHQVEMAELLLSKGAKLDYDGAARKDSNKYKGRTALHYAAMHDNEEMVIMLVRRSSNKDKQDEDGRTPIMLAAKEGCEKTVQYLALNDASLGIVDSMDMTAAQVAEASYHHELAAFLRQVANERHRNDIMRQQIVKSGHGAKSGRQTVKNIKRAGSRKTPTSAASSRETNHLTPPPSDGSFSSPSPHYYPTTTSTPNRMETSPEYMFNHEMAPPVNAMWYTTPPPYQDPNYRHVPPNTAFQNAEQMNGSFYC

Target ORF information:

RefSeq Version	NM_066613.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein glp-1 (glp-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066613.4

ORF Insert Sequence:

ATGCGAGTTC TTCTAATTTT ACTCGCGTTT TTTGCGCCAA TCGCCAGTCA ACTTATGGGT 
GGAGAATGCG GAAGGGAAGG TGCTTGCTCC GTCAATGGAA AATGCTATAA TGGAAAACTG 
ATTGAGACAT ACTGGTGCCG TTGCAAAAAA GGATTCGGAG GTGCTTTCTG TGAACGTGAA 
TGCGATTTGG ATTGTAAACG AGGCGAGAAG TGCATCTACG ATGTTTATGG TGAAAATCCG 
ACGTGTATCT GTCAAGATTG CGAAGACGAG ACTCCTCCAA CAGAACGTAC TCAAAAAGGC 
TGTGAAGAAG GCTATGGAGG TCCTGACTGC AAAACTCCTC TATTTTCGGG AGTAAATCCA 
TGCGATTCGG ATCCTTGCAA CAACGGACTC TGCTATCCAT TCTATGGTGG ATTTCAGTGC 
ATATGCAACA ATGGATATGG AGGATCGTAT TGTGAAGAAG GAATCGATCA TTGTGCTCAA 
AATGAATGCG CAGAAGGTTC AACGTGTGTC AATAGTGTAT ACAACTATTA CTGTGATTGC 
CCAATTGGAA AATCCGGTCG ATATTGTGAA CGAACTGAAT GTGCTTTGAT GGGAAACATT 
TGCAATCATG GAAGATGTAT TCCGAACAGA GATGAAGACA AGAACTTCAG ATGTGTATGC 
GACTCGGGAT ACGAGGGAGA ATTTTGCAAT AAGGATAAAA ACGAATGCCT CATCGAAGAA 
ACGTGTGTTA ACAACTCTAC ATGTTTCAAT TTGCACGGTG ATTTTACTTG TACCTGTAAA 
CCTGGATACG CTGGAAAGTA TTGCGAGGAG GCTATCGACA TGTGCAAGGA TTACGTTTGC 
CAAAATGATG GATACTGTGC CCATGACTCG AATCAGATGC CAATTTGTTA TTGCGAACAA 
GGATTCACTG GACAACGATG TGAGATTGAG TGTCCTTCAG GATTCGGGGG AATTCATTGT 
GATCTTCCAC TACAGAGACC ACACTGCTCT CGGAGCAATG GAACGTGTTA CAACGATGGA 
AGATGTATAA ATGGTTTCTG TGTCTGTGAA CCTGATTATA TTGGAGATCG ATGTGAGATT 
AATAGGAAAG ATTTCAAGTT CCCAGATATT CAGTCATGCA AATACAATCC GTGTGTGAAT 
AATGCGACTT GTATCGATCT CAAAAACAGT GGATATTCAT GTCATTGTCC GCTTGGATTC 
TATGGATTGA ACTGCGAGCA ACATTTGTTG TGCACTCCAA CTACATGTGC TAACGGAGGT 
ACTTGTGAAG GTGTGAATGG CGTTATCAGA TGTAACTGCC CCAACGGATT CTCTGGAGAT 
TACTGCGAAA TAAAGGATAG ACAGTTATGC TCCAGACATC CATGCAAGAA CGGAGGAGTT 
TGTAAGAATA CTGGATATTG CGAATGCCAA TATGGATACA CTGGACCCAC TTGTGAAGAA 
GTTCTAGTTA TCGAGAAATC AAAAGAGACT GTCATTCGTG ATCTATGCGA GCAACGAAAG 
TGTATGGATC TTGCTTCCAA TGGTATTTGC AACCCGGAAT GCAATTTGGA AGAATGTAAT 
TTTGATGGAG GTGATTGCTC AGGAGGGCAA AGACCATTCT CCAAATGTCA ATACCCTGCA 
AGATGTGCTG ATCAATTCGC AAATGGAGTC TGTAATCAGG AATGCAATAA TGAAGAATGT 
CTTTACGATG GATTGGACTG TCAATCAGAG TTATTTCGAT GCCCAGCTCA TATTCGGAAA 
CATTGTATTG AAAGACGAGG CGATGGAGTT TGTAATTTAG AATGCAGTTT CATTGGATGT 
GGTTTTGACG GTGGAGATTG CAATAACGGA ACAGAAGCAA TAATCCTCAG TGATATTCGT 
ATAAAAGTTC AAATTGATCC TATTGAATTC CAAGCTACCG GAGGAGAAAC ACTAATGCAA 
ATCAGTGCAA ATCTTCGAGC AACCGTACGT ATTCAACGGG ATGAACTTGG ACCTCTTGTA 
TTCAGATGGG ATGGTGAACA TGAAATGGAA AGAGTTGAGA TGAATAGTAG CAAGCTCGAA 
GATCAATTTG TACTTTCGCA TCACGTTCGC AGGTATCGGC AAGCTGTTGT CACTGGAATT 
GTCTTGTATT TGGAAGTCGA AGAAATATGT AAACCCGAGT TTTGCCGATT TTCAACAGCG 
CAAAGTGTCG TTGATTTGAT TGCTGCTGGA CTTGTGAAGT CTGATGGTCG TATGTCTCTG 
GGTCTCCCAA TTACGGAAGC AATGGTAGCT GTTCCAAAGA GAAACGAAAT CGATGAAGGA 
TGGTCTCGCA GTCAAGTTAT TCTGTTTGCA TGTATTGCAT TTTTGGCTTT TGGAACTGTT 
GTCGCTGGTG TTATCGCAAA GAATGGTCCA GAAAGAAGTA GAAAAAGAAA AATGGTGAAC 
GCAACAGTCT GGATGCCTCC GATGGAATCA ACGAACGAGA AAGGTCGTCG GAATCAGTCA 
AATCATTCGA GTCAGTGCTC TCTTCTTGAC AATTCTGCCT ACTATCACCC CAATACCAAG 
AGACACTGTA GCGATTATAG TACCGGCTAC AATGGAGAAC AATATTCACA GATTTATCCA 
CAAACTCTCG CAAATGGATA TCCCGGTGAT TACAACGAAT TGAACTTTGA CTTTCAATCT 
GAGACGTTCG CACCAGCTGA TCTACCAGCC GACGAAATCC CTCTCCATGT TCAAGCTGCA 
GGACCAGATG CGATTACAGC TCCAATCACA AATGAGTCAG TCAATCAAGT GGATTCAAAG 
TATAGACGAC GAGTTCTGCA CTGGCTTGCT GCCAATGTCA GAGGAAAACC GGAAGATGTC 
ATTACAACAG AGGCAATTCG ATGCTTGAAG GCTGGAGCCG ATGTGAATGC AAGAGATTGT 
GATGAGAATA CTGCTCTGAT GCTAGCTGTT CGTGCACACC GTGTCAGACT TTCCGTAGTG 
CTTCTTCGTG AAGGAGCTAA TCCAACAATA TTCAATAATT CGGAGCGAAG TGCCCTTCAT 
GAGGCTGTTG TCAACAAGGA TCTGCGTATT CTGCGGCATC TTCTCACTGA TAAACGTCTT 
CTCAAAGAAA TTGATGAATT GGACCGGAAT GGTATGACTG CATTGATGCT AGTAGCACGT 
GAACTCGGAA AACATCAAGT GGAGATGGCA GAGCTTCTTC TTTCAAAAGG AGCAAAACTT 
GATTACGATG GTGCTGCTAG AAAAGATTCC AACAAGTATA AAGGACGAAC AGCTCTTCAT 
TACGCAGCGA TGCACGATAA CGAGGAAATG GTAATAATGC TGGTCCGACG AAGCTCTAAT 
AAGGACAAAC AGGACGAAGA CGGAAGAACA CCGATAATGC TTGCCGCTAA GGAAGGATGT 
GAAAAGACGG TGCAATATTT GGCTCTTAAT GATGCTAGTT TGGGTATAGT CGATTCAATG 
GACATGACTG CTGCTCAAGT TGCTGAAGCC AGCTACCATC ATGAACTTGC GGCTTTTCTT 
CGCCAAGTGG CAAACGAACG GCATCGTAAT GATATCATGC GACAGCAAAT AGTCAAGTCA 
GGTCATGGTG CTAAGTCTGG GCGACAAACT GTGAAGAATA TCAAAAGAGC AGGATCTCGA 
AAAACGCCAA CAAGTGCAGC ATCGTCTCGC GAAACAAATC ATCTGACACC ACCACCGTCT 
GATGGATCGT TCTCATCTCC GTCTCCACAT TATTATCCGA CGACTACATC GACACCGAAT 
CGAATGGAAA CAAGTCCGGA GTACATGTTT AACCATGAAA TGGCACCACC GGTCAATGCG 
ATGTGGTATA CTACACCACC TCCTTATCAA GATCCAAACT ATCGTCATGT GCCTCCAAAT 
ACTGCATTTC AAAATGCAGA GCAAATGAAT GGCTCCTTCT ACTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glp-1 ( NM_066613.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499014.1 Caenorhabditis elegans Protein glp-1 (glp-1), mRNA.

Price & Availability↑

CloneID	OCa341791
Clone ID Related Accession (Same CDS sequence)	NM_066613.4 , NM_066613.5
Accession Version	NM_066613.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein glp-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066613.4.

ATGCGAGTTC TTCTAATTTT ACTCGCGTTT TTTGCGCCAA TCGCCAGTCA ACTTATGGGT 
GGAGAATGCG GAAGGGAAGG TGCTTGCTCC GTCAATGGAA AATGCTATAA TGGAAAACTG 
ATTGAGACAT ACTGGTGCCG TTGCAAAAAA GGATTCGGAG GTGCTTTCTG TGAACGTGAA 
TGCGATTTGG ATTGTAAACG AGGCGAGAAG TGCATCTACG ATGTTTATGG TGAAAATCCG 
ACGTGTATCT GTCAAGATTG CGAAGACGAG ACTCCTCCAA CAGAACGTAC TCAAAAAGGC 
TGTGAAGAAG GCTATGGAGG TCCTGACTGC AAAACTCCTC TATTTTCGGG AGTAAATCCA 
TGCGATTCGG ATCCTTGCAA CAACGGACTC TGCTATCCAT TCTATGGTGG ATTTCAGTGC 
ATATGCAACA ATGGATATGG AGGATCGTAT TGTGAAGAAG GAATCGATCA TTGTGCTCAA 
AATGAATGCG CAGAAGGTTC AACGTGTGTC AATAGTGTAT ACAACTATTA CTGTGATTGC 
CCAATTGGAA AATCCGGTCG ATATTGTGAA CGAACTGAAT GTGCTTTGAT GGGAAACATT 
TGCAATCATG GAAGATGTAT TCCGAACAGA GATGAAGACA AGAACTTCAG ATGTGTATGC 
GACTCGGGAT ACGAGGGAGA ATTTTGCAAT AAGGATAAAA ACGAATGCCT CATCGAAGAA 
ACGTGTGTTA ACAACTCTAC ATGTTTCAAT TTGCACGGTG ATTTTACTTG TACCTGTAAA 
CCTGGATACG CTGGAAAGTA TTGCGAGGAG GCTATCGACA TGTGCAAGGA TTACGTTTGC 
CAAAATGATG GATACTGTGC CCATGACTCG AATCAGATGC CAATTTGTTA TTGCGAACAA 
GGATTCACTG GACAACGATG TGAGATTGAG TGTCCTTCAG GATTCGGGGG AATTCATTGT 
GATCTTCCAC TACAGAGACC ACACTGCTCT CGGAGCAATG GAACGTGTTA CAACGATGGA 
AGATGTATAA ATGGTTTCTG TGTCTGTGAA CCTGATTATA TTGGAGATCG ATGTGAGATT 
AATAGGAAAG ATTTCAAGTT CCCAGATATT CAGTCATGCA AATACAATCC GTGTGTGAAT 
AATGCGACTT GTATCGATCT CAAAAACAGT GGATATTCAT GTCATTGTCC GCTTGGATTC 
TATGGATTGA ACTGCGAGCA ACATTTGTTG TGCACTCCAA CTACATGTGC TAACGGAGGT 
ACTTGTGAAG GTGTGAATGG CGTTATCAGA TGTAACTGCC CCAACGGATT CTCTGGAGAT 
TACTGCGAAA TAAAGGATAG ACAGTTATGC TCCAGACATC CATGCAAGAA CGGAGGAGTT 
TGTAAGAATA CTGGATATTG CGAATGCCAA TATGGATACA CTGGACCCAC TTGTGAAGAA 
GTTCTAGTTA TCGAGAAATC AAAAGAGACT GTCATTCGTG ATCTATGCGA GCAACGAAAG 
TGTATGGATC TTGCTTCCAA TGGTATTTGC AACCCGGAAT GCAATTTGGA AGAATGTAAT 
TTTGATGGAG GTGATTGCTC AGGAGGGCAA AGACCATTCT CCAAATGTCA ATACCCTGCA 
AGATGTGCTG ATCAATTCGC AAATGGAGTC TGTAATCAGG AATGCAATAA TGAAGAATGT 
CTTTACGATG GATTGGACTG TCAATCAGAG TTATTTCGAT GCCCAGCTCA TATTCGGAAA 
CATTGTATTG AAAGACGAGG CGATGGAGTT TGTAATTTAG AATGCAGTTT CATTGGATGT 
GGTTTTGACG GTGGAGATTG CAATAACGGA ACAGAAGCAA TAATCCTCAG TGATATTCGT 
ATAAAAGTTC AAATTGATCC TATTGAATTC CAAGCTACCG GAGGAGAAAC ACTAATGCAA 
ATCAGTGCAA ATCTTCGAGC AACCGTACGT ATTCAACGGG ATGAACTTGG ACCTCTTGTA 
TTCAGATGGG ATGGTGAACA TGAAATGGAA AGAGTTGAGA TGAATAGTAG CAAGCTCGAA 
GATCAATTTG TACTTTCGCA TCACGTTCGC AGGTATCGGC AAGCTGTTGT CACTGGAATT 
GTCTTGTATT TGGAAGTCGA AGAAATATGT AAACCCGAGT TTTGCCGATT TTCAACAGCG 
CAAAGTGTCG TTGATTTGAT TGCTGCTGGA CTTGTGAAGT CTGATGGTCG TATGTCTCTG 
GGTCTCCCAA TTACGGAAGC AATGGTAGCT GTTCCAAAGA GAAACGAAAT CGATGAAGGA 
TGGTCTCGCA GTCAAGTTAT TCTGTTTGCA TGTATTGCAT TTTTGGCTTT TGGAACTGTT 
GTCGCTGGTG TTATCGCAAA GAATGGTCCA GAAAGAAGTA GAAAAAGAAA AATGGTGAAC 
GCAACAGTCT GGATGCCTCC GATGGAATCA ACGAACGAGA AAGGTCGTCG GAATCAGTCA 
AATCATTCGA GTCAGTGCTC TCTTCTTGAC AATTCTGCCT ACTATCACCC CAATACCAAG 
AGACACTGTA GCGATTATAG TACCGGCTAC AATGGAGAAC AATATTCACA GATTTATCCA 
CAAACTCTCG CAAATGGATA TCCCGGTGAT TACAACGAAT TGAACTTTGA CTTTCAATCT 
GAGACGTTCG CACCAGCTGA TCTACCAGCC GACGAAATCC CTCTCCATGT TCAAGCTGCA 
GGACCAGATG CGATTACAGC TCCAATCACA AATGAGTCAG TCAATCAAGT GGATTCAAAG 
TATAGACGAC GAGTTCTGCA CTGGCTTGCT GCCAATGTCA GAGGAAAACC GGAAGATGTC 
ATTACAACAG AGGCAATTCG ATGCTTGAAG GCTGGAGCCG ATGTGAATGC AAGAGATTGT 
GATGAGAATA CTGCTCTGAT GCTAGCTGTT CGTGCACACC GTGTCAGACT TTCCGTAGTG 
CTTCTTCGTG AAGGAGCTAA TCCAACAATA TTCAATAATT CGGAGCGAAG TGCCCTTCAT 
GAGGCTGTTG TCAACAAGGA TCTGCGTATT CTGCGGCATC TTCTCACTGA TAAACGTCTT 
CTCAAAGAAA TTGATGAATT GGACCGGAAT GGTATGACTG CATTGATGCT AGTAGCACGT 
GAACTCGGAA AACATCAAGT GGAGATGGCA GAGCTTCTTC TTTCAAAAGG AGCAAAACTT 
GATTACGATG GTGCTGCTAG AAAAGATTCC AACAAGTATA AAGGACGAAC AGCTCTTCAT 
TACGCAGCGA TGCACGATAA CGAGGAAATG GTAATAATGC TGGTCCGACG AAGCTCTAAT 
AAGGACAAAC AGGACGAAGA CGGAAGAACA CCGATAATGC TTGCCGCTAA GGAAGGATGT 
GAAAAGACGG TGCAATATTT GGCTCTTAAT GATGCTAGTT TGGGTATAGT CGATTCAATG 
GACATGACTG CTGCTCAAGT TGCTGAAGCC AGCTACCATC ATGAACTTGC GGCTTTTCTT 
CGCCAAGTGG CAAACGAACG GCATCGTAAT GATATCATGC GACAGCAAAT AGTCAAGTCA 
GGTCATGGTG CTAAGTCTGG GCGACAAACT GTGAAGAATA TCAAAAGAGC AGGATCTCGA 
AAAACGCCAA CAAGTGCAGC ATCGTCTCGC GAAACAAATC ATCTGACACC ACCACCGTCT 
GATGGATCGT TCTCATCTCC GTCTCCACAT TATTATCCGA CGACTACATC GACACCGAAT 
CGAATGGAAA CAAGTCCGGA GTACATGTTT AACCATGAAA TGGCACCACC GGTCAATGCG 
ATGTGGTATA CTACACCACC TCCTTATCAA GATCCAAACT ATCGTCATGT GCCTCCAAAT 
ACTGCATTTC AAAATGCAGA GCAAATGAAT GGCTCCTTCT ACTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499014.1
CDS	73..3960
Translation	MRVLLILLAFFAPIASQLMGGECGREGACSVNGKCYNGKLIETYWCRCKKGFGGAFCERECDLDCKRGEKCIYDVYGENPTCICQDCEDETPPTERTQKGCEEGYGGPDCKTPLFSGVNPCDSDPCNNGLCYPFYGGFQCICNNGYGGSYCEEGIDHCAQNECAEGSTCVNSVYNYYCDCPIGKSGRYCERTECALMGNICNHGRCIPNRDEDKNFRCVCDSGYEGEFCNKDKNECLIEETCVNNSTCFNLHGDFTCTCKPGYAGKYCEEAIDMCKDYVCQNDGYCAHDSNQMPICYCEQGFTGQRCEIECPSGFGGIHCDLPLQRPHCSRSNGTCYNDGRCINGFCVCEPDYIGDRCEINRKDFKFPDIQSCKYNPCVNNATCIDLKNSGYSCHCPLGFYGLNCEQHLLCTPTTCANGGTCEGVNGVIRCNCPNGFSGDYCEIKDRQLCSRHPCKNGGVCKNTGYCECQYGYTGPTCEEVLVIEKSKETVIRDLCEQRKCMDLASNGICNPECNLEECNFDGGDCSGGQRPFSKCQYPARCADQFANGVCNQECNNEECLYDGLDCQSELFRCPAHIRKHCIERRGDGVCNLECSFIGCGFDGGDCNNGTEAIILSDIRIKVQIDPIEFQATGGETLMQISANLRATVRIQRDELGPLVFRWDGEHEMERVEMNSSKLEDQFVLSHHVRRYRQAVVTGIVLYLEVEEICKPEFCRFSTAQSVVDLIAAGLVKSDGRMSLGLPITEAMVAVPKRNEIDEGWSRSQVILFACIAFLAFGTVVAGVIAKNGPERSRKRKMVNATVWMPPMESTNEKGRRNQSNHSSQCSLLDNSAYYHPNTKRHCSDYSTGYNGEQYSQIYPQTLANGYPGDYNELNFDFQSETFAPADLPADEIPLHVQAAGPDAITAPITNESVNQVDSKYRRRVLHWLAANVRGKPEDVITTEAIRCLKAGADVNARDCDENTALMLAVRAHRVRLSVVLLREGANPTIFNNSERSALHEAVVNKDLRILRHLLTDKRLLKEIDELDRNGMTALMLVARELGKHQVEMAELLLSKGAKLDYDGAARKDSNKYKGRTALHYAAMHDNEEMVIMLVRRSSNKDKQDEDGRTPIMLAAKEGCEKTVQYLALNDASLGIVDSMDMTAAQVAEASYHHELAAFLRQVANERHRNDIMRQQIVKSGHGAKSGRQTVKNIKRAGSRKTPTSAASSRETNHLTPPPSDGSFSSPSPHYYPTTTSTPNRMETSPEYMFNHEMAPPVNAMWYTTPPPYQDPNYRHVPPNTAFQNAEQMNGSFYC

Target ORF information:

RefSeq Version	NM_066613.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein glp-1 (glp-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066613.5

ORF Insert Sequence:

ATGCGAGTTC TTCTAATTTT ACTCGCGTTT TTTGCGCCAA TCGCCAGTCA ACTTATGGGT 
GGAGAATGCG GAAGGGAAGG TGCTTGCTCC GTCAATGGAA AATGCTATAA TGGAAAACTG 
ATTGAGACAT ACTGGTGCCG TTGCAAAAAA GGATTCGGAG GTGCTTTCTG TGAACGTGAA 
TGCGATTTGG ATTGTAAACG AGGCGAGAAG TGCATCTACG ATGTTTATGG TGAAAATCCG 
ACGTGTATCT GTCAAGATTG CGAAGACGAG ACTCCTCCAA CAGAACGTAC TCAAAAAGGC 
TGTGAAGAAG GCTATGGAGG TCCTGACTGC AAAACTCCTC TATTTTCGGG AGTAAATCCA 
TGCGATTCGG ATCCTTGCAA CAACGGACTC TGCTATCCAT TCTATGGTGG ATTTCAGTGC 
ATATGCAACA ATGGATATGG AGGATCGTAT TGTGAAGAAG GAATCGATCA TTGTGCTCAA 
AATGAATGCG CAGAAGGTTC AACGTGTGTC AATAGTGTAT ACAACTATTA CTGTGATTGC 
CCAATTGGAA AATCCGGTCG ATATTGTGAA CGAACTGAAT GTGCTTTGAT GGGAAACATT 
TGCAATCATG GAAGATGTAT TCCGAACAGA GATGAAGACA AGAACTTCAG ATGTGTATGC 
GACTCGGGAT ACGAGGGAGA ATTTTGCAAT AAGGATAAAA ACGAATGCCT CATCGAAGAA 
ACGTGTGTTA ACAACTCTAC ATGTTTCAAT TTGCACGGTG ATTTTACTTG TACCTGTAAA 
CCTGGATACG CTGGAAAGTA TTGCGAGGAG GCTATCGACA TGTGCAAGGA TTACGTTTGC 
CAAAATGATG GATACTGTGC CCATGACTCG AATCAGATGC CAATTTGTTA TTGCGAACAA 
GGATTCACTG GACAACGATG TGAGATTGAG TGTCCTTCAG GATTCGGGGG AATTCATTGT 
GATCTTCCAC TACAGAGACC ACACTGCTCT CGGAGCAATG GAACGTGTTA CAACGATGGA 
AGATGTATAA ATGGTTTCTG TGTCTGTGAA CCTGATTATA TTGGAGATCG ATGTGAGATT 
AATAGGAAAG ATTTCAAGTT CCCAGATATT CAGTCATGCA AATACAATCC GTGTGTGAAT 
AATGCGACTT GTATCGATCT CAAAAACAGT GGATATTCAT GTCATTGTCC GCTTGGATTC 
TATGGATTGA ACTGCGAGCA ACATTTGTTG TGCACTCCAA CTACATGTGC TAACGGAGGT 
ACTTGTGAAG GTGTGAATGG CGTTATCAGA TGTAACTGCC CCAACGGATT CTCTGGAGAT 
TACTGCGAAA TAAAGGATAG ACAGTTATGC TCCAGACATC CATGCAAGAA CGGAGGAGTT 
TGTAAGAATA CTGGATATTG CGAATGCCAA TATGGATACA CTGGACCCAC TTGTGAAGAA 
GTTCTAGTTA TCGAGAAATC AAAAGAGACT GTCATTCGTG ATCTATGCGA GCAACGAAAG 
TGTATGGATC TTGCTTCCAA TGGTATTTGC AACCCGGAAT GCAATTTGGA AGAATGTAAT 
TTTGATGGAG GTGATTGCTC AGGAGGGCAA AGACCATTCT CCAAATGTCA ATACCCTGCA 
AGATGTGCTG ATCAATTCGC AAATGGAGTC TGTAATCAGG AATGCAATAA TGAAGAATGT 
CTTTACGATG GATTGGACTG TCAATCAGAG TTATTTCGAT GCCCAGCTCA TATTCGGAAA 
CATTGTATTG AAAGACGAGG CGATGGAGTT TGTAATTTAG AATGCAGTTT CATTGGATGT 
GGTTTTGACG GTGGAGATTG CAATAACGGA ACAGAAGCAA TAATCCTCAG TGATATTCGT 
ATAAAAGTTC AAATTGATCC TATTGAATTC CAAGCTACCG GAGGAGAAAC ACTAATGCAA 
ATCAGTGCAA ATCTTCGAGC AACCGTACGT ATTCAACGGG ATGAACTTGG ACCTCTTGTA 
TTCAGATGGG ATGGTGAACA TGAAATGGAA AGAGTTGAGA TGAATAGTAG CAAGCTCGAA 
GATCAATTTG TACTTTCGCA TCACGTTCGC AGGTATCGGC AAGCTGTTGT CACTGGAATT 
GTCTTGTATT TGGAAGTCGA AGAAATATGT AAACCCGAGT TTTGCCGATT TTCAACAGCG 
CAAAGTGTCG TTGATTTGAT TGCTGCTGGA CTTGTGAAGT CTGATGGTCG TATGTCTCTG 
GGTCTCCCAA TTACGGAAGC AATGGTAGCT GTTCCAAAGA GAAACGAAAT CGATGAAGGA 
TGGTCTCGCA GTCAAGTTAT TCTGTTTGCA TGTATTGCAT TTTTGGCTTT TGGAACTGTT 
GTCGCTGGTG TTATCGCAAA GAATGGTCCA GAAAGAAGTA GAAAAAGAAA AATGGTGAAC 
GCAACAGTCT GGATGCCTCC GATGGAATCA ACGAACGAGA AAGGTCGTCG GAATCAGTCA 
AATCATTCGA GTCAGTGCTC TCTTCTTGAC AATTCTGCCT ACTATCACCC CAATACCAAG 
AGACACTGTA GCGATTATAG TACCGGCTAC AATGGAGAAC AATATTCACA GATTTATCCA 
CAAACTCTCG CAAATGGATA TCCCGGTGAT TACAACGAAT TGAACTTTGA CTTTCAATCT 
GAGACGTTCG CACCAGCTGA TCTACCAGCC GACGAAATCC CTCTCCATGT TCAAGCTGCA 
GGACCAGATG CGATTACAGC TCCAATCACA AATGAGTCAG TCAATCAAGT GGATTCAAAG 
TATAGACGAC GAGTTCTGCA CTGGCTTGCT GCCAATGTCA GAGGAAAACC GGAAGATGTC 
ATTACAACAG AGGCAATTCG ATGCTTGAAG GCTGGAGCCG ATGTGAATGC AAGAGATTGT 
GATGAGAATA CTGCTCTGAT GCTAGCTGTT CGTGCACACC GTGTCAGACT TTCCGTAGTG 
CTTCTTCGTG AAGGAGCTAA TCCAACAATA TTCAATAATT CGGAGCGAAG TGCCCTTCAT 
GAGGCTGTTG TCAACAAGGA TCTGCGTATT CTGCGGCATC TTCTCACTGA TAAACGTCTT 
CTCAAAGAAA TTGATGAATT GGACCGGAAT GGTATGACTG CATTGATGCT AGTAGCACGT 
GAACTCGGAA AACATCAAGT GGAGATGGCA GAGCTTCTTC TTTCAAAAGG AGCAAAACTT 
GATTACGATG GTGCTGCTAG AAAAGATTCC AACAAGTATA AAGGACGAAC AGCTCTTCAT 
TACGCAGCGA TGCACGATAA CGAGGAAATG GTAATAATGC TGGTCCGACG AAGCTCTAAT 
AAGGACAAAC AGGACGAAGA CGGAAGAACA CCGATAATGC TTGCCGCTAA GGAAGGATGT 
GAAAAGACGG TGCAATATTT GGCTCTTAAT GATGCTAGTT TGGGTATAGT CGATTCAATG 
GACATGACTG CTGCTCAAGT TGCTGAAGCC AGCTACCATC ATGAACTTGC GGCTTTTCTT 
CGCCAAGTGG CAAACGAACG GCATCGTAAT GATATCATGC GACAGCAAAT AGTCAAGTCA 
GGTCATGGTG CTAAGTCTGG GCGACAAACT GTGAAGAATA TCAAAAGAGC AGGATCTCGA 
AAAACGCCAA CAAGTGCAGC ATCGTCTCGC GAAACAAATC ATCTGACACC ACCACCGTCT 
GATGGATCGT TCTCATCTCC GTCTCCACAT TATTATCCGA CGACTACATC GACACCGAAT 
CGAATGGAAA CAAGTCCGGA GTACATGTTT AACCATGAAA TGGCACCACC GGTCAATGCG 
ATGTGGTATA CTACACCACC TCCTTATCAA GATCCAAACT ATCGTCATGT GCCTCCAAAT 
ACTGCATTTC AAAATGCAGA GCAAATGAAT GGCTCCTTCT ACTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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glp-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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