mel-28 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	mel-28
Entrez Gene ID	175634
Full Name	Protein mel-28
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065586.7	NP_497987.2	Protein mel-28
NM_065586.6	NP_497987.2	Protein mel-28

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During nuclear assembly, MEL-28 recruits PP1c to the periphery of decondensed chromatin, where it directs formation of a functional nuclear compartment. Thus, a pool of phosphatase activity associated with a kinetochore-localized nucleoporin contributes to two key events that occur during M-phase exit in metazoans: kinetochore disassembly and nuclear reassembly.
Title: A Nucleoporin Docks Protein Phosphatase 1 to Direct Meiotic Chromosome Segregation and Nuclear Assembly.

the cell can exit the division without reforming the nuclear envelope to uncover an intriguing role of the nucleoporin MEL-28 in mediating chromosome segregation via its interaction with PP1 at the kinetochore
Title: A Nucleoporin at the Meiotic Kinetochore.

MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and acts as a seeding point for postmitotic nuclear pore complex assembly.
Title: MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly.

The following mel-28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mel-28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340018	NM_065586.7 Latest version!	Caenorhabditis elegans Protein mel-28 (mel-28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa340018	NM_065586.6	Caenorhabditis elegans Protein mel-28 (mel-28), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mel-28 ( NM_065586.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497987.2 Caenorhabditis elegans Protein mel-28 (mel-28), mRNA.

Price & Availability↑

CloneID	OCa340018
Clone ID Related Accession (Same CDS sequence)	NM_065586.7 , NM_065586.6
Accession Version	NM_065586.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Protein mel-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065586.6.

ATGGATAATG AAAATTCGTC CATTTTCAAA TCATATCAAG GATACGAGTG TTGGAGGGGA 
GAAAAGCAAA TTATTTTGAA GGATTCAATT GGTCGTCAAC TTCCCTACAT TGTGAATTTT 
AAAAAGAATA CGTGTCAAAT ATTCGATATC GAATGGGAAC GCGTAACTCA CTCGTTCGTT 
TTCCCTGAAG GATGTGCTTT GATCGATGCG GACTATTTTC CAACAGAAGA AGGAAAGCTC 
GGAATTCTTG TTGGTGTTGA AGATCCTCGT CAGTCGTGCG GTGCCGAGCA CTTTGTTCTT 
GCATTGGCAG TTGATCCGGA TTCACCAGCC ATGACAATCA CACACTCACT GGAAGTTCCA 
TCGAAAATTA CAGTTGTGAA GACGCTTTTT TCGTCTGCCG ACATGGCTGA TGAAACACAG 
AGAACAGTTT TAAAGCTCTA TCATCGACTG ATGACGTGGC AGCATATTGT GGCAATTGGT 
TGCAAGGAGA CACAATGCTA CTTAGCTCGT TTAGTTGCTG TTGAGACGCC GTCAAGTCCT 
GTCATCACAG TTCACAGTGA GAAAAAGTAT TTGATCAACC TAATGAATGC TTACGTCAGC 
GGTTCAGTTC TTCAGTACAC TTTGGACGAT GGAGCATATC GTGAATACCC AACCGCCGCC 
GTGTATATCT CAGCGTTATC GTTGATGCCA AGAAGTCGAA CTCTTCTTGT CGGTTTATCG 
ATGGGAGGCA TTCTGGCAGC TTCATTGAAT CCTTCAAATC AGATGATGCT TTTGGAATTG 
AGACACGAAC GACTTGTCAG AAAAATTGCT CCATTAGAAC CAGAAGATGA TCCCGACAAA 
TTTGAGTATT TCATCGCCAC TGTTGATTGT TCACCTCGTC ATCCAATCAT GATTCAACTT 
TGGCGTGGAT CTTTCAAAAC TCTCGAGGAT GTTGATGGAG AAGAAAAGTA CGACCGTCCA 
TCATTCAGTG TTTGTCTAGA ACATAAGATT TTGTTCGGAG AAAGATGGTT GGCTGTGAAT 
CCTATTGTTA CTGAAAGAGA TCATATGATG CTCACAAGAA AGCGAGGAAC GGAAGATTCG 
ATGCACAATG TTTCTCAGAC ATTCGGATCA ACTTCTAATC GAAACTCTGT TCTTTTGGCA 
TACGAACGAA AGAAGATGGT GATCGGCACA GAAGACCCGA ATGCTGAACC AGAATACATT 
GTGGAAGCAG CTATTTTTGA TATAGATTCA TGGTACTACA AAAGAGTTCC CGGTCGAGTA 
TCTACAGATG GAACTGTTCT CAAGCAATGT GCATTCTTAT CAACTATAAA GTCTAACATC 
CGATCAGAAG ATGTAAATGA CATCGGAATT CTTACAAATG AGGCCACTGA TGTATCCAGC 
TTCTCTAGCA TGGTTTCAGA CGCAGATCAA CTGTTTTATC CGTCAGCATT AAGCTTCGAA 
AGGGTTTTTG TTGCCAAGAA TACCAGAATT GACTGGATGA AGATCCAGAA CATCCAGGAC 
ACGATTTTGA ACAAATGCGC CGTCAAACTG CCTGCTCTCA TCAGGAATCC CGAAATGATT 
TCCAGCGTTG TTATGGCAGC TGGTTTAGTG AGAAAAAATA TTCTGTCTGG ATCTCCCAAT 
TCGAGCGCCG CAGAAATAAA CGAACTTCAA TTATCGTCTG ATCAAAAGGT TCTTCTCAAC 
GTTATCGTCT ACTACGGAAA AATTGAAGAG TTTTGTCAAT TGGCATCTCG TCCTGACATT 
AGTGACACGC TAAAACGGGA ATTAGCAGAA TGGGCACTTC ACGAAGCTGT TGACTACAAG 
AGAACTATTT CTGACAAGAT GGTTTCACTC TTCCAGGGAA GATCACTTGC TCTCTCTCCA 
CTAGCAGAAG AATCTATTGC ACAGGGAATC AAGCTTTTCC GAGTTGTTTA CGAATATCTG 
AAAGCCTGTT CAAAAGCGTT AAAAGATGAT CGACTTCGCA ATTTGGCCCA TTCTGTGATA 
TGTATGAGAA ACCATACGAA ACTTACCAGC CAATTCATCA ATTTTGCCAT CATTCCTGTA 
GACCCGATCA GACAGCAAAG AATGAAGGAT TTACATTCAA AGAGAAAAAA TATGGCGAGA 
AAGAACTCAT CATCTCTACC CGTTCAATCA GTCGTTCGAA AAATGAATCG TCAAGCTCCA 
AATGCTCAAT TCTGGAACGA TATTCCACAT GACGAATGGT ATCCGCCAAC ACCTCTCGAT 
TTGCTGGAAT GCCTGCTGAA TGTTTCGATT TCTGAGAGTA TTAAACGAGA ACTCGTTGTC 
CAATATGTCA TTGATTGGAT TTCCACATCT CCTGAAGATT CTGAACACAG CGAAAAACAA 
TTGGCACTTG AAACTATCAA AATCATGACA AATCAAATGC TTAACGTTAA CTTGGAGAAA 
ATTTATTACA TTCTGGACCA AGGAAAGAAG GCTTTAACAA GCAGCAAGAC ATCGGATGAT 
ATGAGAGCAC TTGGAGAGAA AGTATTCTCA ATGAAAGATG ACGAGATTAG TTATGAGAAA 
TTGTGGGGAA AAGATGCTCC GATGACAGTG ACAATCGGAA AACACGATTT ACAGCGATTT 
GAACAACGAA TGAAAATGCA AATGGAAGGT GGAAAAGTTA GATTGCCAGT TCTCGATCCG 
GAATCTGAGA TTCTCTACCA AATGTTTCTC TTTGAAAACG AAAAGTTTGA AGCAATGTCA 
TCAGAAGCGA TTAGTTCCAA CAAATTGCTA TCGGCTTTCC TGCCAGGAAT GATCAAGAAG 
GATGGAAGAG GTCGTCAGAA GACTGCGAAA GAACAGGAAA TCGAAATTTC AGTTAAGAAA 
ATGTTTGAGA GAAAGGTGCA AAACGACGAC GAAGATATGC CTGAAGTTTT CGCTTCTGTC 
AATGACAAAA CAGAAAGAAA GCGGAAATCA AGTCAGTTTG GTGAAGACGA TGAGAGTAGT 
GTTTCGTCAT CACAATATGT GCCACCTACC GCGAAGAGAA TTCAACAGTG GAAGAGTGCC 
GTCGAATCTG TTGCCAACAA TTCGAGTATC AACAGTATCA CAAGTCCGGA TTCTCACCAA 
AATGCAGAAA TCAATATGAT GATTGCAACA CCGGCACGGT ATTACAAGCG ACATAATGAG 
GAGGAAAATG TTCAAGATGG ATTTCTATCG CCCGCTGGAA ACAGACCGCC GCCAGTCTCT 
GCTCACAACA GTATTCTGAA AACAGCTAAA GGTGGCCAAT CGGCTAGTCG AGGCCGTATC 
CGATTCCGTG CTGACGTTCC AAGAGGTGCT GACGAGAGTA TTGAAGACAA TGGTCGGAAA 
GGCCTTGCAT TGAACTTTGC AATTCTTGAA GATGAAGAAG AGGAAACCAT GACTATTAGA 
AAATCACGGT CTATGGGAAA ACATGACGAG GAGAAGGATT CAGAGAAAAA TGTAGTGGAT 
GAGATGGAAG AAGTAAAAGA TCAAGAACAA GAAAACGATG AATGCATTGA AAGTGAGAAA 
ACTTTCGAAA ACCAAGACGA TTTCGAGGTT CTCGAAGATA CATCTGCTCC AGAAGCTGCA 
AATACAGAAA ATGGTTCGGA AACTCCTCCC ATGGAAGATA CGTTTGAAGT ACGAGATGAT 
GACGTCATGC CACCAACCGA TGAGACATAT TTGAGTCATT TACAAACCGA TAAAACGGGT 
ATTCTTGAGG AGGAAGGTGA AGATGAAGAT ATATGGGATG GAGTTCAAAG ATCTTTCGAG 
GTTCAAATGG ATGAGGATTG TGAAGCTGTA CCAACGATTG ATGTAGCCGA TGATTTGGAG 
AGTAAATCTG AAGAAGTGAA TGAAGAAGAG GTAGTTGAAT CTGAAGAAGT TCAGCAAGAT 
GCGAAAGAAC CTGAAAAAAC TGAAAAACGA CAGGAAGAGC CAGAACCTGA AGTTATGCAG 
CCAGTGATTC CGGAAGAGCC TCAAAACGAA TCTCTCGAAT CGTCTATTAA ACTGCAGGAA 
GAGTTACAGG AAGAGCCTGA CATAGTTCCG ACAGGTGATG AAGATACCGC GGATAAAGTT 
CAAGAACAAG CTGTTGAGGA AGATAGACCA CCATCTAGAA ACACTCGCTC ATCATCTGTC 
CAGAAGTCTA CTTCACAAGT GGAAGACCGG GATCCGAAAG AACTTGTCGA GGAAGAAAGA 
CCTCCATCTA GAAACACTCG TTCTGCGTCT GTTCAAAAAT CATCTAACCA AGAGAAAACT 
TCGGAATCAG GAGAAGTCAC GGAAGAAGAT AGACCACCAT CCAGAAATAC GCGGTCGGCA 
TCTGTTCAAA AATCTTCATC AAAGGTAAAA GACCAGAAGC CAGAGGAACT CATCGAGGAA 
GATAGACCTC CATCAAGAAA TACTCGATCC GCATCAGCCC AGAAGACAGT GGCAGCAAAT 
AAATCTGTTT TGGAGTCTGA AATTCCAAGT CGTTCAGCGT CACGAAGAAC TCGCTCCACA 
TCGCTTAGGA ATGATACTGT TGCCGAACCA GATGAGACGT CGGTTGCGAT GACAACTCGG 
CGTCGGACTC GGGCAACTTC TGAGGTTGTT AGCAAGCAGT CGTCTGAAGA TGATGGAAGA 
TCGACACCAA AAACCGGAAG AACTCCCACG AAAAAGGCTG CAGCTAGTAC ATCTTCTAGT 
AGAGCTGGAA GTGTAACTCG CGGTAAAAAA TCGATCATCC AAAAGATGCC ATCCCCGCTA 
GAGGTTACAA TGGAAGTTCA AGAAGAAGAA GAAGAAGAAG CGGAGGAGGA ACGGCCTGCA 
TCAAGAAGCA CTCGCTCTGC ATCTGTCAAA AATACAACTG TTGACCCATC GTCATCTGCA 
CTTGCTTCTA CCAAACGTAC AACTTCTAGA AAGAGGGGCA ATTCGGAAAC GATCGACTTT 
AATCAGGACG ACAAATCGGC ACCGACGACG CCAAAAAGAG GAAGACCCGC CAAAAAGGAT 
GCAGGATCTC CTAAAGTTGG AAGTAAAGCT CGTGGCACCA AGCCTAAATC GATATTCGAA 
AATCAAGAAG ATGAAGAAGA TCGTTCATCC TCTCCAGATA TCGAACAACC AGCCACACCT 
ACACGGTCTT CCAAACGAAC GGCTAGATCC AGAGCAAATT CCGAATCGAT CGATGATGAT 
AGCAAGCAGA AGACCCCAAA AAAGAAAAAT GCCGCGGTTA ATGAAGCTGG TACCAGTAAA 
CAGTCTAGAA GTGTGACGAG ATCTCGAGCA TCATCTATTG ATGTACAACA AGAGGTGGAA 
GAGCCAACCA CTCCAAAACG CGGAAGAGGA AGACCGCCCA AAACCGTTCT GGAAAATATT 
GAAGAGGGTG AAGAAGAGAG AAAAGAAACG GCTGCGACTC CTCTACTGCG TTCAGCTCGC 
CGTGCTAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497987.2
CDS	2..5356
Translation	MDNENSSIFKSYQGYECWRGEKQIILKDSIGRQLPYIVNFKKNTCQIFDIEWERVTHSFVFPEGCALIDADYFPTEEGKLGILVGVEDPRQSCGAEHFVLALAVDPDSPAMTITHSLEVPSKITVVKTLFSSADMADETQRTVLKLYHRLMTWQHIVAIGCKETQCYLARLVAVETPSSPVITVHSEKKYLINLMNAYVSGSVLQYTLDDGAYREYPTAAVYISALSLMPRSRTLLVGLSMGGILAASLNPSNQMMLLELRHERLVRKIAPLEPEDDPDKFEYFIATVDCSPRHPIMIQLWRGSFKTLEDVDGEEKYDRPSFSVCLEHKILFGERWLAVNPIVTERDHMMLTRKRGTEDSMHNVSQTFGSTSNRNSVLLAYERKKMVIGTEDPNAEPEYIVEAAIFDIDSWYYKRVPGRVSTDGTVLKQCAFLSTIKSNIRSEDVNDIGILTNEATDVSSFSSMVSDADQLFYPSALSFERVFVAKNTRIDWMKIQNIQDTILNKCAVKLPALIRNPEMISSVVMAAGLVRKNILSGSPNSSAAEINELQLSSDQKVLLNVIVYYGKIEEFCQLASRPDISDTLKRELAEWALHEAVDYKRTISDKMVSLFQGRSLALSPLAEESIAQGIKLFRVVYEYLKACSKALKDDRLRNLAHSVICMRNHTKLTSQFINFAIIPVDPIRQQRMKDLHSKRKNMARKNSSSLPVQSVVRKMNRQAPNAQFWNDIPHDEWYPPTPLDLLECLLNVSISESIKRELVVQYVIDWISTSPEDSEHSEKQLALETIKIMTNQMLNVNLEKIYYILDQGKKALTSSKTSDDMRALGEKVFSMKDDEISYEKLWGKDAPMTVTIGKHDLQRFEQRMKMQMEGGKVRLPVLDPESEILYQMFLFENEKFEAMSSEAISSNKLLSAFLPGMIKKDGRGRQKTAKEQEIEISVKKMFERKVQNDDEDMPEVFASVNDKTERKRKSSQFGEDDESSVSSSQYVPPTAKRIQQWKSAVESVANNSSINSITSPDSHQNAEINMMIATPARYYKRHNEEENVQDGFLSPAGNRPPPVSAHNSILKTAKGGQSASRGRIRFRADVPRGADESIEDNGRKGLALNFAILEDEEEETMTIRKSRSMGKHDEEKDSEKNVVDEMEEVKDQEQENDECIESEKTFENQDDFEVLEDTSAPEAANTENGSETPPMEDTFEVRDDDVMPPTDETYLSHLQTDKTGILEEEGEDEDIWDGVQRSFEVQMDEDCEAVPTIDVADDLESKSEEVNEEEVVESEEVQQDAKEPEKTEKRQEEPEPEVMQPVIPEEPQNESLESSIKLQEELQEEPDIVPTGDEDTADKVQEQAVEEDRPPSRNTRSSSVQKSTSQVEDRDPKELVEEERPPSRNTRSASVQKSSNQEKTSESGEVTEEDRPPSRNTRSASVQKSSSKVKDQKPEELIEEDRPPSRNTRSASAQKTVAANKSVLESEIPSRSASRRTRSTSLRNDTVAEPDETSVAMTTRRRTRATSEVVSKQSSEDDGRSTPKTGRTPTKKAAASTSSSRAGSVTRGKKSIIQKMPSPLEVTMEVQEEEEEEAEEERPASRSTRSASVKNTTVDPSSSALASTKRTTSRKRGNSETIDFNQDDKSAPTTPKRGRPAKKDAGSPKVGSKARGTKPKSIFENQEDEEDRSSSPDIEQPATPTRSSKRTARSRANSESIDDDSKQKTPKKKNAAVNEAGTSKQSRSVTRSRASSIDVQQEVEEPTTPKRGRGRPPKTVLENIEEGEEERKETAATPLLRSARRAKQ

Target ORF information:

RefSeq Version	NM_065586.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein mel-28 (mel-28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065586.7

ORF Insert Sequence:

ATGGATAATG AAAATTCGTC CATTTTCAAA TCATATCAAG GATACGAGTG TTGGAGGGGA 
GAAAAGCAAA TTATTTTGAA GGATTCAATT GGTCGTCAAC TTCCCTACAT TGTGAATTTT 
AAAAAGAATA CGTGTCAAAT ATTCGATATC GAATGGGAAC GCGTAACTCA CTCGTTCGTT 
TTCCCTGAAG GATGTGCTTT GATCGATGCG GACTATTTTC CAACAGAAGA AGGAAAGCTC 
GGAATTCTTG TTGGTGTTGA AGATCCTCGT CAGTCGTGCG GTGCCGAGCA CTTTGTTCTT 
GCATTGGCAG TTGATCCGGA TTCACCAGCC ATGACAATCA CACACTCACT GGAAGTTCCA 
TCGAAAATTA CAGTTGTGAA GACGCTTTTT TCGTCTGCCG ACATGGCTGA TGAAACACAG 
AGAACAGTTT TAAAGCTCTA TCATCGACTG ATGACGTGGC AGCATATTGT GGCAATTGGT 
TGCAAGGAGA CACAATGCTA CTTAGCTCGT TTAGTTGCTG TTGAGACGCC GTCAAGTCCT 
GTCATCACAG TTCACAGTGA GAAAAAGTAT TTGATCAACC TAATGAATGC TTACGTCAGC 
GGTTCAGTTC TTCAGTACAC TTTGGACGAT GGAGCATATC GTGAATACCC AACCGCCGCC 
GTGTATATCT CAGCGTTATC GTTGATGCCA AGAAGTCGAA CTCTTCTTGT CGGTTTATCG 
ATGGGAGGCA TTCTGGCAGC TTCATTGAAT CCTTCAAATC AGATGATGCT TTTGGAATTG 
AGACACGAAC GACTTGTCAG AAAAATTGCT CCATTAGAAC CAGAAGATGA TCCCGACAAA 
TTTGAGTATT TCATCGCCAC TGTTGATTGT TCACCTCGTC ATCCAATCAT GATTCAACTT 
TGGCGTGGAT CTTTCAAAAC TCTCGAGGAT GTTGATGGAG AAGAAAAGTA CGACCGTCCA 
TCATTCAGTG TTTGTCTAGA ACATAAGATT TTGTTCGGAG AAAGATGGTT GGCTGTGAAT 
CCTATTGTTA CTGAAAGAGA TCATATGATG CTCACAAGAA AGCGAGGAAC GGAAGATTCG 
ATGCACAATG TTTCTCAGAC ATTCGGATCA ACTTCTAATC GAAACTCTGT TCTTTTGGCA 
TACGAACGAA AGAAGATGGT GATCGGCACA GAAGACCCGA ATGCTGAACC AGAATACATT 
GTGGAAGCAG CTATTTTTGA TATAGATTCA TGGTACTACA AAAGAGTTCC CGGTCGAGTA 
TCTACAGATG GAACTGTTCT CAAGCAATGT GCATTCTTAT CAACTATAAA GTCTAACATC 
CGATCAGAAG ATGTAAATGA CATCGGAATT CTTACAAATG AGGCCACTGA TGTATCCAGC 
TTCTCTAGCA TGGTTTCAGA CGCAGATCAA CTGTTTTATC CGTCAGCATT AAGCTTCGAA 
AGGGTTTTTG TTGCCAAGAA TACCAGAATT GACTGGATGA AGATCCAGAA CATCCAGGAC 
ACGATTTTGA ACAAATGCGC CGTCAAACTG CCTGCTCTCA TCAGGAATCC CGAAATGATT 
TCCAGCGTTG TTATGGCAGC TGGTTTAGTG AGAAAAAATA TTCTGTCTGG ATCTCCCAAT 
TCGAGCGCCG CAGAAATAAA CGAACTTCAA TTATCGTCTG ATCAAAAGGT TCTTCTCAAC 
GTTATCGTCT ACTACGGAAA AATTGAAGAG TTTTGTCAAT TGGCATCTCG TCCTGACATT 
AGTGACACGC TAAAACGGGA ATTAGCAGAA TGGGCACTTC ACGAAGCTGT TGACTACAAG 
AGAACTATTT CTGACAAGAT GGTTTCACTC TTCCAGGGAA GATCACTTGC TCTCTCTCCA 
CTAGCAGAAG AATCTATTGC ACAGGGAATC AAGCTTTTCC GAGTTGTTTA CGAATATCTG 
AAAGCCTGTT CAAAAGCGTT AAAAGATGAT CGACTTCGCA ATTTGGCCCA TTCTGTGATA 
TGTATGAGAA ACCATACGAA ACTTACCAGC CAATTCATCA ATTTTGCCAT CATTCCTGTA 
GACCCGATCA GACAGCAAAG AATGAAGGAT TTACATTCAA AGAGAAAAAA TATGGCGAGA 
AAGAACTCAT CATCTCTACC CGTTCAATCA GTCGTTCGAA AAATGAATCG TCAAGCTCCA 
AATGCTCAAT TCTGGAACGA TATTCCACAT GACGAATGGT ATCCGCCAAC ACCTCTCGAT 
TTGCTGGAAT GCCTGCTGAA TGTTTCGATT TCTGAGAGTA TTAAACGAGA ACTCGTTGTC 
CAATATGTCA TTGATTGGAT TTCCACATCT CCTGAAGATT CTGAACACAG CGAAAAACAA 
TTGGCACTTG AAACTATCAA AATCATGACA AATCAAATGC TTAACGTTAA CTTGGAGAAA 
ATTTATTACA TTCTGGACCA AGGAAAGAAG GCTTTAACAA GCAGCAAGAC ATCGGATGAT 
ATGAGAGCAC TTGGAGAGAA AGTATTCTCA ATGAAAGATG ACGAGATTAG TTATGAGAAA 
TTGTGGGGAA AAGATGCTCC GATGACAGTG ACAATCGGAA AACACGATTT ACAGCGATTT 
GAACAACGAA TGAAAATGCA AATGGAAGGT GGAAAAGTTA GATTGCCAGT TCTCGATCCG 
GAATCTGAGA TTCTCTACCA AATGTTTCTC TTTGAAAACG AAAAGTTTGA AGCAATGTCA 
TCAGAAGCGA TTAGTTCCAA CAAATTGCTA TCGGCTTTCC TGCCAGGAAT GATCAAGAAG 
GATGGAAGAG GTCGTCAGAA GACTGCGAAA GAACAGGAAA TCGAAATTTC AGTTAAGAAA 
ATGTTTGAGA GAAAGGTGCA AAACGACGAC GAAGATATGC CTGAAGTTTT CGCTTCTGTC 
AATGACAAAA CAGAAAGAAA GCGGAAATCA AGTCAGTTTG GTGAAGACGA TGAGAGTAGT 
GTTTCGTCAT CACAATATGT GCCACCTACC GCGAAGAGAA TTCAACAGTG GAAGAGTGCC 
GTCGAATCTG TTGCCAACAA TTCGAGTATC AACAGTATCA CAAGTCCGGA TTCTCACCAA 
AATGCAGAAA TCAATATGAT GATTGCAACA CCGGCACGGT ATTACAAGCG ACATAATGAG 
GAGGAAAATG TTCAAGATGG ATTTCTATCG CCCGCTGGAA ACAGACCGCC GCCAGTCTCT 
GCTCACAACA GTATTCTGAA AACAGCTAAA GGTGGCCAAT CGGCTAGTCG AGGCCGTATC 
CGATTCCGTG CTGACGTTCC AAGAGGTGCT GACGAGAGTA TTGAAGACAA TGGTCGGAAA 
GGCCTTGCAT TGAACTTTGC AATTCTTGAA GATGAAGAAG AGGAAACCAT GACTATTAGA 
AAATCACGGT CTATGGGAAA ACATGACGAG GAGAAGGATT CAGAGAAAAA TGTAGTGGAT 
GAGATGGAAG AAGTAAAAGA TCAAGAACAA GAAAACGATG AATGCATTGA AAGTGAGAAA 
ACTTTCGAAA ACCAAGACGA TTTCGAGGTT CTCGAAGATA CATCTGCTCC AGAAGCTGCA 
AATACAGAAA ATGGTTCGGA AACTCCTCCC ATGGAAGATA CGTTTGAAGT ACGAGATGAT 
GACGTCATGC CACCAACCGA TGAGACATAT TTGAGTCATT TACAAACCGA TAAAACGGGT 
ATTCTTGAGG AGGAAGGTGA AGATGAAGAT ATATGGGATG GAGTTCAAAG ATCTTTCGAG 
GTTCAAATGG ATGAGGATTG TGAAGCTGTA CCAACGATTG ATGTAGCCGA TGATTTGGAG 
AGTAAATCTG AAGAAGTGAA TGAAGAAGAG GTAGTTGAAT CTGAAGAAGT TCAGCAAGAT 
GCGAAAGAAC CTGAAAAAAC TGAAAAACGA CAGGAAGAGC CAGAACCTGA AGTTATGCAG 
CCAGTGATTC CGGAAGAGCC TCAAAACGAA TCTCTCGAAT CGTCTATTAA ACTGCAGGAA 
GAGTTACAGG AAGAGCCTGA CATAGTTCCG ACAGGTGATG AAGATACCGC GGATAAAGTT 
CAAGAACAAG CTGTTGAGGA AGATAGACCA CCATCTAGAA ACACTCGCTC ATCATCTGTC 
CAGAAGTCTA CTTCACAAGT GGAAGACCGG GATCCGAAAG AACTTGTCGA GGAAGAAAGA 
CCTCCATCTA GAAACACTCG TTCTGCGTCT GTTCAAAAAT CATCTAACCA AGAGAAAACT 
TCGGAATCAG GAGAAGTCAC GGAAGAAGAT AGACCACCAT CCAGAAATAC GCGGTCGGCA 
TCTGTTCAAA AATCTTCATC AAAGGTAAAA GACCAGAAGC CAGAGGAACT CATCGAGGAA 
GATAGACCTC CATCAAGAAA TACTCGATCC GCATCAGCCC AGAAGACAGT GGCAGCAAAT 
AAATCTGTTT TGGAGTCTGA AATTCCAAGT CGTTCAGCGT CACGAAGAAC TCGCTCCACA 
TCGCTTAGGA ATGATACTGT TGCCGAACCA GATGAGACGT CGGTTGCGAT GACAACTCGG 
CGTCGGACTC GGGCAACTTC TGAGGTTGTT AGCAAGCAGT CGTCTGAAGA TGATGGAAGA 
TCGACACCAA AAACCGGAAG AACTCCCACG AAAAAGGCTG CAGCTAGTAC ATCTTCTAGT 
AGAGCTGGAA GTGTAACTCG CGGTAAAAAA TCGATCATCC AAAAGATGCC ATCCCCGCTA 
GAGGTTACAA TGGAAGTTCA AGAAGAAGAA GAAGAAGAAG CGGAGGAGGA ACGGCCTGCA 
TCAAGAAGCA CTCGCTCTGC ATCTGTCAAA AATACAACTG TTGACCCATC GTCATCTGCA 
CTTGCTTCTA CCAAACGTAC AACTTCTAGA AAGAGGGGCA ATTCGGAAAC GATCGACTTT 
AATCAGGACG ACAAATCGGC ACCGACGACG CCAAAAAGAG GAAGACCCGC CAAAAAGGAT 
GCAGGATCTC CTAAAGTTGG AAGTAAAGCT CGTGGCACCA AGCCTAAATC GATATTCGAA 
AATCAAGAAG ATGAAGAAGA TCGTTCATCC TCTCCAGATA TCGAACAACC AGCCACACCT 
ACACGGTCTT CCAAACGAAC GGCTAGATCC AGAGCAAATT CCGAATCGAT CGATGATGAT 
AGCAAGCAGA AGACCCCAAA AAAGAAAAAT GCCGCGGTTA ATGAAGCTGG TACCAGTAAA 
CAGTCTAGAA GTGTGACGAG ATCTCGAGCA TCATCTATTG ATGTACAACA AGAGGTGGAA 
GAGCCAACCA CTCCAAAACG CGGAAGAGGA AGACCGCCCA AAACCGTTCT GGAAAATATT 
GAAGAGGGTG AAGAAGAGAG AAAAGAAACG GCTGCGACTC CTCTACTGCG TTCAGCTCGC 
CGTGCTAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mel-28 ( NM_065586.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_497987.2 Caenorhabditis elegans Protein mel-28 (mel-28), partial mRNA.

Price & Availability↑

CloneID	OCa340018
Clone ID Related Accession (Same CDS sequence)	NM_065586.7 , NM_065586.6
Accession Version	NM_065586.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Protein mel-28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065586.5. COMPLETENESS: incomplete on both ends.

ATGGATAATG AAAATTCGTC CATTTTCAAA TCATATCAAG GATACGAGTG TTGGAGGGGA 
GAAAAGCAAA TTATTTTGAA GGATTCAATT GGTCGTCAAC TTCCCTACAT TGTGAATTTT 
AAAAAGAATA CGTGTCAAAT ATTCGATATC GAATGGGAAC GCGTAACTCA CTCGTTCGTT 
TTCCCTGAAG GATGTGCTTT GATCGATGCG GACTATTTTC CAACAGAAGA AGGAAAGCTC 
GGAATTCTTG TTGGTGTTGA AGATCCTCGT CAGTCGTGCG GTGCCGAGCA CTTTGTTCTT 
GCATTGGCAG TTGATCCGGA TTCACCAGCC ATGACAATCA CACACTCACT GGAAGTTCCA 
TCGAAAATTA CAGTTGTGAA GACGCTTTTT TCGTCTGCCG ACATGGCTGA TGAAACACAG 
AGAACAGTTT TAAAGCTCTA TCATCGACTG ATGACGTGGC AGCATATTGT GGCAATTGGT 
TGCAAGGAGA CACAATGCTA CTTAGCTCGT TTAGTTGCTG TTGAGACGCC GTCAAGTCCT 
GTCATCACAG TTCACAGTGA GAAAAAGTAT TTGATCAACC TAATGAATGC TTACGTCAGC 
GGTTCAGTTC TTCAGTACAC TTTGGACGAT GGAGCATATC GTGAATACCC AACCGCCGCC 
GTGTATATCT CAGCGTTATC GTTGATGCCA AGAAGTCGAA CTCTTCTTGT CGGTTTATCG 
ATGGGAGGCA TTCTGGCAGC TTCATTGAAT CCTTCAAATC AGATGATGCT TTTGGAATTG 
AGACACGAAC GACTTGTCAG AAAAATTGCT CCATTAGAAC CAGAAGATGA TCCCGACAAA 
TTTGAGTATT TCATCGCCAC TGTTGATTGT TCACCTCGTC ATCCAATCAT GATTCAACTT 
TGGCGTGGAT CTTTCAAAAC TCTCGAGGAT GTTGATGGAG AAGAAAAGTA CGACCGTCCA 
TCATTCAGTG TTTGTCTAGA ACATAAGATT TTGTTCGGAG AAAGATGGTT GGCTGTGAAT 
CCTATTGTTA CTGAAAGAGA TCATATGATG CTCACAAGAA AGCGAGGAAC GGAAGATTCG 
ATGCACAATG TTTCTCAGAC ATTCGGATCA ACTTCTAATC GAAACTCTGT TCTTTTGGCA 
TACGAACGAA AGAAGATGGT GATCGGCACA GAAGACCCGA ATGCTGAACC AGAATACATT 
GTGGAAGCAG CTATTTTTGA TATAGATTCA TGGTACTACA AAAGAGTTCC CGGTCGAGTA 
TCTACAGATG GAACTGTTCT CAAGCAATGT GCATTCTTAT CAACTATAAA GTCTAACATC 
CGATCAGAAG ATGTAAATGA CATCGGAATT CTTACAAATG AGGCCACTGA TGTATCCAGC 
TTCTCTAGCA TGGTTTCAGA CGCAGATCAA CTGTTTTATC CGTCAGCATT AAGCTTCGAA 
AGGGTTTTTG TTGCCAAGAA TACCAGAATT GACTGGATGA AGATCCAGAA CATCCAGGAC 
ACGATTTTGA ACAAATGCGC CGTCAAACTG CCTGCTCTCA TCAGGAATCC CGAAATGATT 
TCCAGCGTTG TTATGGCAGC TGGTTTAGTG AGAAAAAATA TTCTGTCTGG ATCTCCCAAT 
TCGAGCGCCG CAGAAATAAA CGAACTTCAA TTATCGTCTG ATCAAAAGGT TCTTCTCAAC 
GTTATCGTCT ACTACGGAAA AATTGAAGAG TTTTGTCAAT TGGCATCTCG TCCTGACATT 
AGTGACACGC TAAAACGGGA ATTAGCAGAA TGGGCACTTC ACGAAGCTGT TGACTACAAG 
AGAACTATTT CTGACAAGAT GGTTTCACTC TTCCAGGGAA GATCACTTGC TCTCTCTCCA 
CTAGCAGAAG AATCTATTGC ACAGGGAATC AAGCTTTTCC GAGTTGTTTA CGAATATCTG 
AAAGCCTGTT CAAAAGCGTT AAAAGATGAT CGACTTCGCA ATTTGGCCCA TTCTGTGATA 
TGTATGAGAA ACCATACGAA ACTTACCAGC CAATTCATCA ATTTTGCCAT CATTCCTGTA 
GACCCGATCA GACAGCAAAG AATGAAGGAT TTACATTCAA AGAGAAAAAA TATGGCGAGA 
AAGAACTCAT CATCTCTACC CGTTCAATCA GTCGTTCGAA AAATGAATCG TCAAGCTCCA 
AATGCTCAAT TCTGGAACGA TATTCCACAT GACGAATGGT ATCCGCCAAC ACCTCTCGAT 
TTGCTGGAAT GCCTGCTGAA TGTTTCGATT TCTGAGAGTA TTAAACGAGA ACTCGTTGTC 
CAATATGTCA TTGATTGGAT TTCCACATCT CCTGAAGATT CTGAACACAG CGAAAAACAA 
TTGGCACTTG AAACTATCAA AATCATGACA AATCAAATGC TTAACGTTAA CTTGGAGAAA 
ATTTATTACA TTCTGGACCA AGGAAAGAAG GCTTTAACAA GCAGCAAGAC ATCGGATGAT 
ATGAGAGCAC TTGGAGAGAA AGTATTCTCA ATGAAAGATG ACGAGATTAG TTATGAGAAA 
TTGTGGGGAA AAGATGCTCC GATGACAGTG ACAATCGGAA AACACGATTT ACAGCGATTT 
GAACAACGAA TGAAAATGCA AATGGAAGGT GGAAAAGTTA GATTGCCAGT TCTCGATCCG 
GAATCTGAGA TTCTCTACCA AATGTTTCTC TTTGAAAACG AAAAGTTTGA AGCAATGTCA 
TCAGAAGCGA TTAGTTCCAA CAAATTGCTA TCGGCTTTCC TGCCAGGAAT GATCAAGAAG 
GATGGAAGAG GTCGTCAGAA GACTGCGAAA GAACAGGAAA TCGAAATTTC AGTTAAGAAA 
ATGTTTGAGA GAAAGGTGCA AAACGACGAC GAAGATATGC CTGAAGTTTT CGCTTCTGTC 
AATGACAAAA CAGAAAGAAA GCGGAAATCA AGTCAGTTTG GTGAAGACGA TGAGAGTAGT 
GTTTCGTCAT CACAATATGT GCCACCTACC GCGAAGAGAA TTCAACAGTG GAAGAGTGCC 
GTCGAATCTG TTGCCAACAA TTCGAGTATC AACAGTATCA CAAGTCCGGA TTCTCACCAA 
AATGCAGAAA TCAATATGAT GATTGCAACA CCGGCACGGT ATTACAAGCG ACATAATGAG 
GAGGAAAATG TTCAAGATGG ATTTCTATCG CCCGCTGGAA ACAGACCGCC GCCAGTCTCT 
GCTCACAACA GTATTCTGAA AACAGCTAAA GGTGGCCAAT CGGCTAGTCG AGGCCGTATC 
CGATTCCGTG CTGACGTTCC AAGAGGTGCT GACGAGAGTA TTGAAGACAA TGGTCGGAAA 
GGCCTTGCAT TGAACTTTGC AATTCTTGAA GATGAAGAAG AGGAAACCAT GACTATTAGA 
AAATCACGGT CTATGGGAAA ACATGACGAG GAGAAGGATT CAGAGAAAAA TGTAGTGGAT 
GAGATGGAAG AAGTAAAAGA TCAAGAACAA GAAAACGATG AATGCATTGA AAGTGAGAAA 
ACTTTCGAAA ACCAAGACGA TTTCGAGGTT CTCGAAGATA CATCTGCTCC AGAAGCTGCA 
AATACAGAAA ATGGTTCGGA AACTCCTCCC ATGGAAGATA CGTTTGAAGT ACGAGATGAT 
GACGTCATGC CACCAACCGA TGAGACATAT TTGAGTCATT TACAAACCGA TAAAACGGGT 
ATTCTTGAGG AGGAAGGTGA AGATGAAGAT ATATGGGATG GAGTTCAAAG ATCTTTCGAG 
GTTCAAATGG ATGAGGATTG TGAAGCTGTA CCAACGATTG ATGTAGCCGA TGATTTGGAG 
AGTAAATCTG AAGAAGTGAA TGAAGAAGAG GTAGTTGAAT CTGAAGAAGT TCAGCAAGAT 
GCGAAAGAAC CTGAAAAAAC TGAAAAACGA CAGGAAGAGC CAGAACCTGA AGTTATGCAG 
CCAGTGATTC CGGAAGAGCC TCAAAACGAA TCTCTCGAAT CGTCTATTAA ACTGCAGGAA 
GAGTTACAGG AAGAGCCTGA CATAGTTCCG ACAGGTGATG AAGATACCGC GGATAAAGTT 
CAAGAACAAG CTGTTGAGGA AGATAGACCA CCATCTAGAA ACACTCGCTC ATCATCTGTC 
CAGAAGTCTA CTTCACAAGT GGAAGACCGG GATCCGAAAG AACTTGTCGA GGAAGAAAGA 
CCTCCATCTA GAAACACTCG TTCTGCGTCT GTTCAAAAAT CATCTAACCA AGAGAAAACT 
TCGGAATCAG GAGAAGTCAC GGAAGAAGAT AGACCACCAT CCAGAAATAC GCGGTCGGCA 
TCTGTTCAAA AATCTTCATC AAAGGTAAAA GACCAGAAGC CAGAGGAACT CATCGAGGAA 
GATAGACCTC CATCAAGAAA TACTCGATCC GCATCAGCCC AGAAGACAGT GGCAGCAAAT 
AAATCTGTTT TGGAGTCTGA AATTCCAAGT CGTTCAGCGT CACGAAGAAC TCGCTCCACA 
TCGCTTAGGA ATGATACTGT TGCCGAACCA GATGAGACGT CGGTTGCGAT GACAACTCGG 
CGTCGGACTC GGGCAACTTC TGAGGTTGTT AGCAAGCAGT CGTCTGAAGA TGATGGAAGA 
TCGACACCAA AAACCGGAAG AACTCCCACG AAAAAGGCTG CAGCTAGTAC ATCTTCTAGT 
AGAGCTGGAA GTGTAACTCG CGGTAAAAAA TCGATCATCC AAAAGATGCC ATCCCCGCTA 
GAGGTTACAA TGGAAGTTCA AGAAGAAGAA GAAGAAGAAG CGGAGGAGGA ACGGCCTGCA 
TCAAGAAGCA CTCGCTCTGC ATCTGTCAAA AATACAACTG TTGACCCATC GTCATCTGCA 
CTTGCTTCTA CCAAACGTAC AACTTCTAGA AAGAGGGGCA ATTCGGAAAC GATCGACTTT 
AATCAGGACG ACAAATCGGC ACCGACGACG CCAAAAAGAG GAAGACCCGC CAAAAAGGAT 
GCAGGATCTC CTAAAGTTGG AAGTAAAGCT CGTGGCACCA AGCCTAAATC GATATTCGAA 
AATCAAGAAG ATGAAGAAGA TCGTTCATCC TCTCCAGATA TCGAACAACC AGCCACACCT 
ACACGGTCTT CCAAACGAAC GGCTAGATCC AGAGCAAATT CCGAATCGAT CGATGATGAT 
AGCAAGCAGA AGACCCCAAA AAAGAAAAAT GCCGCGGTTA ATGAAGCTGG TACCAGTAAA 
CAGTCTAGAA GTGTGACGAG ATCTCGAGCA TCATCTATTG ATGTACAACA AGAGGTGGAA 
GAGCCAACCA CTCCAAAACG CGGAAGAGGA AGACCGCCCA AAACCGTTCT GGAAAATATT 
GAAGAGGGTG AAGAAGAGAG AAAAGAAACG GCTGCGACTC CTCTACTGCG TTCAGCTCGC 
CGTGCTAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_497987.2
CDS	1..5355
Translation	MDNENSSIFKSYQGYECWRGEKQIILKDSIGRQLPYIVNFKKNTCQIFDIEWERVTHSFVFPEGCALIDADYFPTEEGKLGILVGVEDPRQSCGAEHFVLALAVDPDSPAMTITHSLEVPSKITVVKTLFSSADMADETQRTVLKLYHRLMTWQHIVAIGCKETQCYLARLVAVETPSSPVITVHSEKKYLINLMNAYVSGSVLQYTLDDGAYREYPTAAVYISALSLMPRSRTLLVGLSMGGILAASLNPSNQMMLLELRHERLVRKIAPLEPEDDPDKFEYFIATVDCSPRHPIMIQLWRGSFKTLEDVDGEEKYDRPSFSVCLEHKILFGERWLAVNPIVTERDHMMLTRKRGTEDSMHNVSQTFGSTSNRNSVLLAYERKKMVIGTEDPNAEPEYIVEAAIFDIDSWYYKRVPGRVSTDGTVLKQCAFLSTIKSNIRSEDVNDIGILTNEATDVSSFSSMVSDADQLFYPSALSFERVFVAKNTRIDWMKIQNIQDTILNKCAVKLPALIRNPEMISSVVMAAGLVRKNILSGSPNSSAAEINELQLSSDQKVLLNVIVYYGKIEEFCQLASRPDISDTLKRELAEWALHEAVDYKRTISDKMVSLFQGRSLALSPLAEESIAQGIKLFRVVYEYLKACSKALKDDRLRNLAHSVICMRNHTKLTSQFINFAIIPVDPIRQQRMKDLHSKRKNMARKNSSSLPVQSVVRKMNRQAPNAQFWNDIPHDEWYPPTPLDLLECLLNVSISESIKRELVVQYVIDWISTSPEDSEHSEKQLALETIKIMTNQMLNVNLEKIYYILDQGKKALTSSKTSDDMRALGEKVFSMKDDEISYEKLWGKDAPMTVTIGKHDLQRFEQRMKMQMEGGKVRLPVLDPESEILYQMFLFENEKFEAMSSEAISSNKLLSAFLPGMIKKDGRGRQKTAKEQEIEISVKKMFERKVQNDDEDMPEVFASVNDKTERKRKSSQFGEDDESSVSSSQYVPPTAKRIQQWKSAVESVANNSSINSITSPDSHQNAEINMMIATPARYYKRHNEEENVQDGFLSPAGNRPPPVSAHNSILKTAKGGQSASRGRIRFRADVPRGADESIEDNGRKGLALNFAILEDEEEETMTIRKSRSMGKHDEEKDSEKNVVDEMEEVKDQEQENDECIESEKTFENQDDFEVLEDTSAPEAANTENGSETPPMEDTFEVRDDDVMPPTDETYLSHLQTDKTGILEEEGEDEDIWDGVQRSFEVQMDEDCEAVPTIDVADDLESKSEEVNEEEVVESEEVQQDAKEPEKTEKRQEEPEPEVMQPVIPEEPQNESLESSIKLQEELQEEPDIVPTGDEDTADKVQEQAVEEDRPPSRNTRSSSVQKSTSQVEDRDPKELVEEERPPSRNTRSASVQKSSNQEKTSESGEVTEEDRPPSRNTRSASVQKSSSKVKDQKPEELIEEDRPPSRNTRSASAQKTVAANKSVLESEIPSRSASRRTRSTSLRNDTVAEPDETSVAMTTRRRTRATSEVVSKQSSEDDGRSTPKTGRTPTKKAAASTSSSRAGSVTRGKKSIIQKMPSPLEVTMEVQEEEEEEAEEERPASRSTRSASVKNTTVDPSSSALASTKRTTSRKRGNSETIDFNQDDKSAPTTPKRGRPAKKDAGSPKVGSKARGTKPKSIFENQEDEEDRSSSPDIEQPATPTRSSKRTARSRANSESIDDDSKQKTPKKKNAAVNEAGTSKQSRSVTRSRASSIDVQQEVEEPTTPKRGRGRPPKTVLENIEEGEEERKETAATPLLRSARRAKQ

Target ORF information:

RefSeq Version	NM_065586.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Protein mel-28 (mel-28), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065586.6

ORF Insert Sequence:

ATGGATAATG AAAATTCGTC CATTTTCAAA TCATATCAAG GATACGAGTG TTGGAGGGGA 
GAAAAGCAAA TTATTTTGAA GGATTCAATT GGTCGTCAAC TTCCCTACAT TGTGAATTTT 
AAAAAGAATA CGTGTCAAAT ATTCGATATC GAATGGGAAC GCGTAACTCA CTCGTTCGTT 
TTCCCTGAAG GATGTGCTTT GATCGATGCG GACTATTTTC CAACAGAAGA AGGAAAGCTC 
GGAATTCTTG TTGGTGTTGA AGATCCTCGT CAGTCGTGCG GTGCCGAGCA CTTTGTTCTT 
GCATTGGCAG TTGATCCGGA TTCACCAGCC ATGACAATCA CACACTCACT GGAAGTTCCA 
TCGAAAATTA CAGTTGTGAA GACGCTTTTT TCGTCTGCCG ACATGGCTGA TGAAACACAG 
AGAACAGTTT TAAAGCTCTA TCATCGACTG ATGACGTGGC AGCATATTGT GGCAATTGGT 
TGCAAGGAGA CACAATGCTA CTTAGCTCGT TTAGTTGCTG TTGAGACGCC GTCAAGTCCT 
GTCATCACAG TTCACAGTGA GAAAAAGTAT TTGATCAACC TAATGAATGC TTACGTCAGC 
GGTTCAGTTC TTCAGTACAC TTTGGACGAT GGAGCATATC GTGAATACCC AACCGCCGCC 
GTGTATATCT CAGCGTTATC GTTGATGCCA AGAAGTCGAA CTCTTCTTGT CGGTTTATCG 
ATGGGAGGCA TTCTGGCAGC TTCATTGAAT CCTTCAAATC AGATGATGCT TTTGGAATTG 
AGACACGAAC GACTTGTCAG AAAAATTGCT CCATTAGAAC CAGAAGATGA TCCCGACAAA 
TTTGAGTATT TCATCGCCAC TGTTGATTGT TCACCTCGTC ATCCAATCAT GATTCAACTT 
TGGCGTGGAT CTTTCAAAAC TCTCGAGGAT GTTGATGGAG AAGAAAAGTA CGACCGTCCA 
TCATTCAGTG TTTGTCTAGA ACATAAGATT TTGTTCGGAG AAAGATGGTT GGCTGTGAAT 
CCTATTGTTA CTGAAAGAGA TCATATGATG CTCACAAGAA AGCGAGGAAC GGAAGATTCG 
ATGCACAATG TTTCTCAGAC ATTCGGATCA ACTTCTAATC GAAACTCTGT TCTTTTGGCA 
TACGAACGAA AGAAGATGGT GATCGGCACA GAAGACCCGA ATGCTGAACC AGAATACATT 
GTGGAAGCAG CTATTTTTGA TATAGATTCA TGGTACTACA AAAGAGTTCC CGGTCGAGTA 
TCTACAGATG GAACTGTTCT CAAGCAATGT GCATTCTTAT CAACTATAAA GTCTAACATC 
CGATCAGAAG ATGTAAATGA CATCGGAATT CTTACAAATG AGGCCACTGA TGTATCCAGC 
TTCTCTAGCA TGGTTTCAGA CGCAGATCAA CTGTTTTATC CGTCAGCATT AAGCTTCGAA 
AGGGTTTTTG TTGCCAAGAA TACCAGAATT GACTGGATGA AGATCCAGAA CATCCAGGAC 
ACGATTTTGA ACAAATGCGC CGTCAAACTG CCTGCTCTCA TCAGGAATCC CGAAATGATT 
TCCAGCGTTG TTATGGCAGC TGGTTTAGTG AGAAAAAATA TTCTGTCTGG ATCTCCCAAT 
TCGAGCGCCG CAGAAATAAA CGAACTTCAA TTATCGTCTG ATCAAAAGGT TCTTCTCAAC 
GTTATCGTCT ACTACGGAAA AATTGAAGAG TTTTGTCAAT TGGCATCTCG TCCTGACATT 
AGTGACACGC TAAAACGGGA ATTAGCAGAA TGGGCACTTC ACGAAGCTGT TGACTACAAG 
AGAACTATTT CTGACAAGAT GGTTTCACTC TTCCAGGGAA GATCACTTGC TCTCTCTCCA 
CTAGCAGAAG AATCTATTGC ACAGGGAATC AAGCTTTTCC GAGTTGTTTA CGAATATCTG 
AAAGCCTGTT CAAAAGCGTT AAAAGATGAT CGACTTCGCA ATTTGGCCCA TTCTGTGATA 
TGTATGAGAA ACCATACGAA ACTTACCAGC CAATTCATCA ATTTTGCCAT CATTCCTGTA 
GACCCGATCA GACAGCAAAG AATGAAGGAT TTACATTCAA AGAGAAAAAA TATGGCGAGA 
AAGAACTCAT CATCTCTACC CGTTCAATCA GTCGTTCGAA AAATGAATCG TCAAGCTCCA 
AATGCTCAAT TCTGGAACGA TATTCCACAT GACGAATGGT ATCCGCCAAC ACCTCTCGAT 
TTGCTGGAAT GCCTGCTGAA TGTTTCGATT TCTGAGAGTA TTAAACGAGA ACTCGTTGTC 
CAATATGTCA TTGATTGGAT TTCCACATCT CCTGAAGATT CTGAACACAG CGAAAAACAA 
TTGGCACTTG AAACTATCAA AATCATGACA AATCAAATGC TTAACGTTAA CTTGGAGAAA 
ATTTATTACA TTCTGGACCA AGGAAAGAAG GCTTTAACAA GCAGCAAGAC ATCGGATGAT 
ATGAGAGCAC TTGGAGAGAA AGTATTCTCA ATGAAAGATG ACGAGATTAG TTATGAGAAA 
TTGTGGGGAA AAGATGCTCC GATGACAGTG ACAATCGGAA AACACGATTT ACAGCGATTT 
GAACAACGAA TGAAAATGCA AATGGAAGGT GGAAAAGTTA GATTGCCAGT TCTCGATCCG 
GAATCTGAGA TTCTCTACCA AATGTTTCTC TTTGAAAACG AAAAGTTTGA AGCAATGTCA 
TCAGAAGCGA TTAGTTCCAA CAAATTGCTA TCGGCTTTCC TGCCAGGAAT GATCAAGAAG 
GATGGAAGAG GTCGTCAGAA GACTGCGAAA GAACAGGAAA TCGAAATTTC AGTTAAGAAA 
ATGTTTGAGA GAAAGGTGCA AAACGACGAC GAAGATATGC CTGAAGTTTT CGCTTCTGTC 
AATGACAAAA CAGAAAGAAA GCGGAAATCA AGTCAGTTTG GTGAAGACGA TGAGAGTAGT 
GTTTCGTCAT CACAATATGT GCCACCTACC GCGAAGAGAA TTCAACAGTG GAAGAGTGCC 
GTCGAATCTG TTGCCAACAA TTCGAGTATC AACAGTATCA CAAGTCCGGA TTCTCACCAA 
AATGCAGAAA TCAATATGAT GATTGCAACA CCGGCACGGT ATTACAAGCG ACATAATGAG 
GAGGAAAATG TTCAAGATGG ATTTCTATCG CCCGCTGGAA ACAGACCGCC GCCAGTCTCT 
GCTCACAACA GTATTCTGAA AACAGCTAAA GGTGGCCAAT CGGCTAGTCG AGGCCGTATC 
CGATTCCGTG CTGACGTTCC AAGAGGTGCT GACGAGAGTA TTGAAGACAA TGGTCGGAAA 
GGCCTTGCAT TGAACTTTGC AATTCTTGAA GATGAAGAAG AGGAAACCAT GACTATTAGA 
AAATCACGGT CTATGGGAAA ACATGACGAG GAGAAGGATT CAGAGAAAAA TGTAGTGGAT 
GAGATGGAAG AAGTAAAAGA TCAAGAACAA GAAAACGATG AATGCATTGA AAGTGAGAAA 
ACTTTCGAAA ACCAAGACGA TTTCGAGGTT CTCGAAGATA CATCTGCTCC AGAAGCTGCA 
AATACAGAAA ATGGTTCGGA AACTCCTCCC ATGGAAGATA CGTTTGAAGT ACGAGATGAT 
GACGTCATGC CACCAACCGA TGAGACATAT TTGAGTCATT TACAAACCGA TAAAACGGGT 
ATTCTTGAGG AGGAAGGTGA AGATGAAGAT ATATGGGATG GAGTTCAAAG ATCTTTCGAG 
GTTCAAATGG ATGAGGATTG TGAAGCTGTA CCAACGATTG ATGTAGCCGA TGATTTGGAG 
AGTAAATCTG AAGAAGTGAA TGAAGAAGAG GTAGTTGAAT CTGAAGAAGT TCAGCAAGAT 
GCGAAAGAAC CTGAAAAAAC TGAAAAACGA CAGGAAGAGC CAGAACCTGA AGTTATGCAG 
CCAGTGATTC CGGAAGAGCC TCAAAACGAA TCTCTCGAAT CGTCTATTAA ACTGCAGGAA 
GAGTTACAGG AAGAGCCTGA CATAGTTCCG ACAGGTGATG AAGATACCGC GGATAAAGTT 
CAAGAACAAG CTGTTGAGGA AGATAGACCA CCATCTAGAA ACACTCGCTC ATCATCTGTC 
CAGAAGTCTA CTTCACAAGT GGAAGACCGG GATCCGAAAG AACTTGTCGA GGAAGAAAGA 
CCTCCATCTA GAAACACTCG TTCTGCGTCT GTTCAAAAAT CATCTAACCA AGAGAAAACT 
TCGGAATCAG GAGAAGTCAC GGAAGAAGAT AGACCACCAT CCAGAAATAC GCGGTCGGCA 
TCTGTTCAAA AATCTTCATC AAAGGTAAAA GACCAGAAGC CAGAGGAACT CATCGAGGAA 
GATAGACCTC CATCAAGAAA TACTCGATCC GCATCAGCCC AGAAGACAGT GGCAGCAAAT 
AAATCTGTTT TGGAGTCTGA AATTCCAAGT CGTTCAGCGT CACGAAGAAC TCGCTCCACA 
TCGCTTAGGA ATGATACTGT TGCCGAACCA GATGAGACGT CGGTTGCGAT GACAACTCGG 
CGTCGGACTC GGGCAACTTC TGAGGTTGTT AGCAAGCAGT CGTCTGAAGA TGATGGAAGA 
TCGACACCAA AAACCGGAAG AACTCCCACG AAAAAGGCTG CAGCTAGTAC ATCTTCTAGT 
AGAGCTGGAA GTGTAACTCG CGGTAAAAAA TCGATCATCC AAAAGATGCC ATCCCCGCTA 
GAGGTTACAA TGGAAGTTCA AGAAGAAGAA GAAGAAGAAG CGGAGGAGGA ACGGCCTGCA 
TCAAGAAGCA CTCGCTCTGC ATCTGTCAAA AATACAACTG TTGACCCATC GTCATCTGCA 
CTTGCTTCTA CCAAACGTAC AACTTCTAGA AAGAGGGGCA ATTCGGAAAC GATCGACTTT 
AATCAGGACG ACAAATCGGC ACCGACGACG CCAAAAAGAG GAAGACCCGC CAAAAAGGAT 
GCAGGATCTC CTAAAGTTGG AAGTAAAGCT CGTGGCACCA AGCCTAAATC GATATTCGAA 
AATCAAGAAG ATGAAGAAGA TCGTTCATCC TCTCCAGATA TCGAACAACC AGCCACACCT 
ACACGGTCTT CCAAACGAAC GGCTAGATCC AGAGCAAATT CCGAATCGAT CGATGATGAT 
AGCAAGCAGA AGACCCCAAA AAAGAAAAAT GCCGCGGTTA ATGAAGCTGG TACCAGTAAA 
CAGTCTAGAA GTGTGACGAG ATCTCGAGCA TCATCTATTG ATGTACAACA AGAGGTGGAA 
GAGCCAACCA CTCCAAAACG CGGAAGAGGA AGACCGCCCA AAACCGTTCT GGAAAATATT 
GAAGAGGGTG AAGAAGAGAG AAAAGAAACG GCTGCGACTC CTCTACTGCG TTCAGCTCGC 
CGTGCTAAAC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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mel-28 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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