ect-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	ect-2
Entrez Gene ID	174658
Full Name	ECT2 (mammalian Rho GEF) homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063917.6	NP_496318.1	ECT2 (mammalian Rho GEF) homolog
NM_063917.5	NP_496318.1	ECT2 (mammalian Rho GEF) homolog
NM_063918.2	NP_496319.1	ECT2 (mammalian Rho GEF) homolog
NM_063918.1	NP_496319.1	ECT2 (mammalian Rho GEF) homolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polarity in the C. elegans zygote is initiated by redundant ECT-2- and PAR-2-dependent mechanisms that lower PAR-3 levels locally, triggering a positive-feedback loop that polarizes the entire cortex.
Title: Symmetry breaking and polarization of the C. elegans zygote by the polarity protein PAR-2.

The following ect-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ect-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338235	NM_063917.6 Latest version!	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCa339602	NM_063918.2 Latest version!	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OCa338235	NM_063917.5	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa339602	NM_063918.1	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ect-2 ( NM_063917.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496318.1 Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.

Price & Availability↑

CloneID	OCa338235
Clone ID Related Accession (Same CDS sequence)	NM_063917.6 , NM_063917.5
Accession Version	NM_063917.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ECT2 (mammalian Rho GEF) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063917.5.

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGTCAGTGA ACGATCTACA CAAAACGAAT GGTCACACAA TGAGTCGTTT 
ATGCCACCAC CACAGTCACA GAGTCGAATG CGACGAGCGT TCAGTAATGC TCAACTCACA 
CTTGCCACCA CGCTGGGCTT TGGCAGAAAT CAGAGTCGAA ATAACTTGGG ACAAATTAAC 
GAGAACACGA CATTTCTGCA GTCTCCCAGA GTTTCGTGTG ATCCAGCAGC TCTCGCAAAC 
ATGACGTCAG TCGGCGGATT GCCTGGATGC CAAAGCGAAT CTGAATCGAC GCCAAAGCGA 
GGAATTCGTT CTCGCCTCAC CTCGACAACG TTCCTCGGCG GCCGACACAA CATACTCGGA 
CGAAACACGT CGATGCGCCG TCAGGCTACC GGATTTGCTC ATTCGGAAAA GGAACTACCT 
CGCTCAAAAA CATCTTCATA TCGTGTCACC GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496318.1
CDS	29..2827
Translation	MDVSMLQSPQKRIKTIQKLAVSKKDVHDFKDIFEMLEEQKEKRNINIIQYDSLDDLSGDWSEYTFFCGNFDSDLFRTLTKHQETQALFIIGASVLKNKLLKNEDLMTLRAARPLYCELMKDVTMKLAAEVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEAKVQSISLVGVPTMRADWVTECWKHRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPTLATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPLESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYIYSNDDVENSCKSPRQPSKRLRVCMEMVETEKNYLSLLKLVVKFKEALECEISHNEFMSRSDVAMMFGKLDPILELHERIFARLTVMSNEANTLLTVVNGKKNDDKNLDFAQVWIDAKEEMKKAYPQYINSYDTIKRLFDKQDRENSKFHTFCKAKESNPEFHRLKLTDLMVKPVQRLPSVILLLKEMAKKSDSKLKNNAEEAAKAIDEVLKTANKTREKNDNFISHLSKFTDIENVPPILVAANRMFIRELVVSPIASTSPGLQQFAKMKLFLFHDVLIITKIRSEKNTMQRLARHASFASLHSRQRRPYRYIDQIQLNTMRSAFRIRAPDEIAKCLELQQQNGSTKPNVFVWCLIHRDEQGGDIDTVFESNDDEEVRDFLDDIHVKILSDCGRFFYSSDPSDMNEINDAMVSDMTTRYFRNLCGAKPQYGHGSQMNITVSERSTQNEWSHNESFMPPPQSQSRMRRAFSNAQLTLATTLGFGRNQSRNNLGQINENTTFLQSPRVSCDPAALANMTSVGGLPGCQSESESTPKRGIRSRLTSTTFLGGRHNILGRNTSMRRQATGFAHSEKELPRSKTSSYRVTDI

Target ORF information:

RefSeq Version	NM_063917.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063917.6

ORF Insert Sequence:

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGTCAGTGA ACGATCTACA CAAAACGAAT GGTCACACAA TGAGTCGTTT 
ATGCCACCAC CACAGTCACA GAGTCGAATG CGACGAGCGT TCAGTAATGC TCAACTCACA 
CTTGCCACCA CGCTGGGCTT TGGCAGAAAT CAGAGTCGAA ATAACTTGGG ACAAATTAAC 
GAGAACACGA CATTTCTGCA GTCTCCCAGA GTTTCGTGTG ATCCAGCAGC TCTCGCAAAC 
ATGACGTCAG TCGGCGGATT GCCTGGATGC CAAAGCGAAT CTGAATCGAC GCCAAAGCGA 
GGAATTCGTT CTCGCCTCAC CTCGACAACG TTCCTCGGCG GCCGACACAA CATACTCGGA 
CGAAACACGT CGATGCGCCG TCAGGCTACC GGATTTGCTC ATTCGGAAAA GGAACTACCT 
CGCTCAAAAA CATCTTCATA TCGTGTCACC GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect-2 ( NM_063917.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496318.1 Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.

Price & Availability↑

CloneID	OCa338235
Clone ID Related Accession (Same CDS sequence)	NM_063917.6 , NM_063917.5
Accession Version	NM_063917.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	ECT2 (mammalian Rho GEF) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063917.4. COMPLETENESS: incomplete on both ends.

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGTCAGTGA ACGATCTACA CAAAACGAAT GGTCACACAA TGAGTCGTTT 
ATGCCACCAC CACAGTCACA GAGTCGAATG CGACGAGCGT TCAGTAATGC TCAACTCACA 
CTTGCCACCA CGCTGGGCTT TGGCAGAAAT CAGAGTCGAA ATAACTTGGG ACAAATTAAC 
GAGAACACGA CATTTCTGCA GTCTCCCAGA GTTTCGTGTG ATCCAGCAGC TCTCGCAAAC 
ATGACGTCAG TCGGCGGATT GCCTGGATGC CAAAGCGAAT CTGAATCGAC GCCAAAGCGA 
GGAATTCGTT CTCGCCTCAC CTCGACAACG TTCCTCGGCG GCCGACACAA CATACTCGGA 
CGAAACACGT CGATGCGCCG TCAGGCTACC GGATTTGCTC ATTCGGAAAA GGAACTACCT 
CGCTCAAAAA CATCTTCATA TCGTGTCACC GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496318.1
CDS	1..2799
Translation	MDVSMLQSPQKRIKTIQKLAVSKKDVHDFKDIFEMLEEQKEKRNINIIQYDSLDDLSGDWSEYTFFCGNFDSDLFRTLTKHQETQALFIIGASVLKNKLLKNEDLMTLRAARPLYCELMKDVTMKLAAEVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEAKVQSISLVGVPTMRADWVTECWKHRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPTLATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPLESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYIYSNDDVENSCKSPRQPSKRLRVCMEMVETEKNYLSLLKLVVKFKEALECEISHNEFMSRSDVAMMFGKLDPILELHERIFARLTVMSNEANTLLTVVNGKKNDDKNLDFAQVWIDAKEEMKKAYPQYINSYDTIKRLFDKQDRENSKFHTFCKAKESNPEFHRLKLTDLMVKPVQRLPSVILLLKEMAKKSDSKLKNNAEEAAKAIDEVLKTANKTREKNDNFISHLSKFTDIENVPPILVAANRMFIRELVVSPIASTSPGLQQFAKMKLFLFHDVLIITKIRSEKNTMQRLARHASFASLHSRQRRPYRYIDQIQLNTMRSAFRIRAPDEIAKCLELQQQNGSTKPNVFVWCLIHRDEQGGDIDTVFESNDDEEVRDFLDDIHVKILSDCGRFFYSSDPSDMNEINDAMVSDMTTRYFRNLCGAKPQYGHGSQMNITVSERSTQNEWSHNESFMPPPQSQSRMRRAFSNAQLTLATTLGFGRNQSRNNLGQINENTTFLQSPRVSCDPAALANMTSVGGLPGCQSESESTPKRGIRSRLTSTTFLGGRHNILGRNTSMRRQATGFAHSEKELPRSKTSSYRVTDI

Target ORF information:

RefSeq Version	NM_063917.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063917.5

ORF Insert Sequence:

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGTCAGTGA ACGATCTACA CAAAACGAAT GGTCACACAA TGAGTCGTTT 
ATGCCACCAC CACAGTCACA GAGTCGAATG CGACGAGCGT TCAGTAATGC TCAACTCACA 
CTTGCCACCA CGCTGGGCTT TGGCAGAAAT CAGAGTCGAA ATAACTTGGG ACAAATTAAC 
GAGAACACGA CATTTCTGCA GTCTCCCAGA GTTTCGTGTG ATCCAGCAGC TCTCGCAAAC 
ATGACGTCAG TCGGCGGATT GCCTGGATGC CAAAGCGAAT CTGAATCGAC GCCAAAGCGA 
GGAATTCGTT CTCGCCTCAC CTCGACAACG TTCCTCGGCG GCCGACACAA CATACTCGGA 
CGAAACACGT CGATGCGCCG TCAGGCTACC GGATTTGCTC ATTCGGAAAA GGAACTACCT 
CGCTCAAAAA CATCTTCATA TCGTGTCACC GATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect-2 ( NM_063918.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496319.1 Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.

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CloneID	OCa339602
Clone ID Related Accession (Same CDS sequence)	NM_063918.2 , NM_063918.1
Accession Version	NM_063918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	ECT2 (mammalian Rho GEF) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063918.1.

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGAATGGTC ACACAATGAG TCGTTTATGC CACCACCACA GTCACAGAGT 
CGAATGCGAC GAGCGTTCAG TAATGCTCAA CTCACACTTG CCACCACGCT GGGCTTTGGC 
AGAAATCAGA GTCGAAATAA CTTGGGACAA ATTAACGAGA ACACGACATT TCTGCAGTCT 
CCCAGAGTTT CGTGTGATCC AGCAGCTCTC GCAAACATGA CGTCAGTCGG CGGATTGCCT 
GGATGCCAAA GCGAATCTGA ATCGACGCCA AAGCGAGGAA TTCGTTCTCG CCTCACCTCG 
ACAACGTTCC TCGGCGGCCG ACACAACATA CTCGGACGAA ACACGTCGAT GCGCCGTCAG 
GCTACCGGAT TTGCTCATTC GGAAAAGGAA CTACCTCGCT CAAAAACATC TTCATATCGT 
GTCACCGATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496319.1
CDS	28..2802
Translation	MDVSMLQSPQKRIKTIQKLAVSKKDVHDFKDIFEMLEEQKEKRNINIIQYDSLDDLSGDWSEYTFFCGNFDSDLFRTLTKHQETQALFIIGASVLKNKLLKNEDLMTLRAARPLYCELMKDVTMKLAAEVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEAKVQSISLVGVPTMRADWVTECWKHRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPTLATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPLESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYIYSNDDVENSCKSPRQPSKRLRVCMEMVETEKNYLSLLKLVVKFKEALECEISHNEFMSRSDVAMMFGKLDPILELHERIFARLTVMSNEANTLLTVVNGKKNDDKNLDFAQVWIDAKEEMKKAYPQYINSYDTIKRLFDKQDRENSKFHTFCKAKESNPEFHRLKLTDLMVKPVQRLPSVILLLKEMAKKSDSKLKNNAEEAAKAIDEVLKTANKTREKNDNFISHLSKFTDIENVPPILVAANRMFIRELVVSPIASTSPGLQQFAKMKLFLFHDVLIITKIRSEKNTMQRLARHASFASLHSRQRRPYRYIDQIQLNTMRSAFRIRAPDEIAKCLELQQQNGSTKPNVFVWCLIHRDEQGGDIDTVFESNDDEEVRDFLDDIHVKILSDCGRFFYSSDPSDMNEINDAMVSDMTTRYFRNLCGAKPQYGHGSQMNITEWSHNESFMPPPQSQSRMRRAFSNAQLTLATTLGFGRNQSRNNLGQINENTTFLQSPRVSCDPAALANMTSVGGLPGCQSESESTPKRGIRSRLTSTTFLGGRHNILGRNTSMRRQATGFAHSEKELPRSKTSSYRVTDI

Target ORF information:

RefSeq Version	NM_063918.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063918.2

ORF Insert Sequence:

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGAATGGTC ACACAATGAG TCGTTTATGC CACCACCACA GTCACAGAGT 
CGAATGCGAC GAGCGTTCAG TAATGCTCAA CTCACACTTG CCACCACGCT GGGCTTTGGC 
AGAAATCAGA GTCGAAATAA CTTGGGACAA ATTAACGAGA ACACGACATT TCTGCAGTCT 
CCCAGAGTTT CGTGTGATCC AGCAGCTCTC GCAAACATGA CGTCAGTCGG CGGATTGCCT 
GGATGCCAAA GCGAATCTGA ATCGACGCCA AAGCGAGGAA TTCGTTCTCG CCTCACCTCG 
ACAACGTTCC TCGGCGGCCG ACACAACATA CTCGGACGAA ACACGTCGAT GCGCCGTCAG 
GCTACCGGAT TTGCTCATTC GGAAAAGGAA CTACCTCGCT CAAAAACATC TTCATATCGT 
GTCACCGATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ect-2 ( NM_063918.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496319.1 Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.

Price & Availability↑

CloneID	OCa339602
Clone ID Related Accession (Same CDS sequence)	NM_063918.2 , NM_063918.1
Accession Version	NM_063918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	ECT2 (mammalian Rho GEF) homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). COMPLETENESS: incomplete on both ends.

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGAATGGTC ACACAATGAG TCGTTTATGC CACCACCACA GTCACAGAGT 
CGAATGCGAC GAGCGTTCAG TAATGCTCAA CTCACACTTG CCACCACGCT GGGCTTTGGC 
AGAAATCAGA GTCGAAATAA CTTGGGACAA ATTAACGAGA ACACGACATT TCTGCAGTCT 
CCCAGAGTTT CGTGTGATCC AGCAGCTCTC GCAAACATGA CGTCAGTCGG CGGATTGCCT 
GGATGCCAAA GCGAATCTGA ATCGACGCCA AAGCGAGGAA TTCGTTCTCG CCTCACCTCG 
ACAACGTTCC TCGGCGGCCG ACACAACATA CTCGGACGAA ACACGTCGAT GCGCCGTCAG 
GCTACCGGAT TTGCTCATTC GGAAAAGGAA CTACCTCGCT CAAAAACATC TTCATATCGT 
GTCACCGATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496319.1
CDS	1..2775
Translation	MDVSMLQSPQKRIKTIQKLAVSKKDVHDFKDIFEMLEEQKEKRNINIIQYDSLDDLSGDWSEYTFFCGNFDSDLFRTLTKHQETQALFIIGASVLKNKLLKNEDLMTLRAARPLYCELMKDVTMKLAAEVPNKRELVDLVHYMGGSVRKDTVSRTNVFIAAKVEAKVQSISLVGVPTMRADWVTECWKHRDDSYFDVMEPCFVDKHRLGVFEGLSLFFHGFKQTEIDDMLRSLENTGGKLAPSPTLATHVVYNPDNDEVETLAVSSNQVHVTKEWFWVSLHRGCCAIEDNFALPTGKLHRKPLESSSVSSPIGKSLRSQNSRSLSSMRDCSVEGPTSLNMTPDYIYSNDDVENSCKSPRQPSKRLRVCMEMVETEKNYLSLLKLVVKFKEALECEISHNEFMSRSDVAMMFGKLDPILELHERIFARLTVMSNEANTLLTVVNGKKNDDKNLDFAQVWIDAKEEMKKAYPQYINSYDTIKRLFDKQDRENSKFHTFCKAKESNPEFHRLKLTDLMVKPVQRLPSVILLLKEMAKKSDSKLKNNAEEAAKAIDEVLKTANKTREKNDNFISHLSKFTDIENVPPILVAANRMFIRELVVSPIASTSPGLQQFAKMKLFLFHDVLIITKIRSEKNTMQRLARHASFASLHSRQRRPYRYIDQIQLNTMRSAFRIRAPDEIAKCLELQQQNGSTKPNVFVWCLIHRDEQGGDIDTVFESNDDEEVRDFLDDIHVKILSDCGRFFYSSDPSDMNEINDAMVSDMTTRYFRNLCGAKPQYGHGSQMNITEWSHNESFMPPPQSQSRMRRAFSNAQLTLATTLGFGRNQSRNNLGQINENTTFLQSPRVSCDPAALANMTSVGGLPGCQSESESTPKRGIRSRLTSTTFLGGRHNILGRNTSMRRQATGFAHSEKELPRSKTSSYRVTDI

Target ORF information:

RefSeq Version	NM_063918.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans ECT2 (mammalian Rho GEF) homolog (ect-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063918.1

ORF Insert Sequence:

ATGGACGTCT CAATGTTGCA ATCACCACAA AAACGAATAA AAACTATTCA AAAACTGGCT 
GTTTCGAAAA AAGATGTTCA TGATTTTAAA GATATTTTCG AGATGCTTGA AGAGCAAAAA 
GAGAAGCGAA ATATAAATAT TATTCAATAC GACTCGTTGG ATGACCTGAG TGGAGATTGG 
AGCGAGTACA CATTCTTTTG TGGAAACTTT GATTCTGACC TCTTTCGAAC ACTAACAAAA 
CATCAGGAAA CGCAAGCACT CTTTATTATT GGGGCGTCCG TGTTGAAAAA TAAGCTACTG 
AAAAATGAGG ATTTGATGAC GTTGCGAGCT GCCCGACCAC TCTACTGCGA GCTTATGAAA 
GATGTAACAA TGAAGTTAGC AGCAGAAGTA CCAAATAAGC GAGAACTCGT TGACTTGGTT 
CATTATATGG GCGGTAGTGT GAGGAAAGAT ACAGTATCAC GAACAAATGT GTTCATCGCA 
GCGAAAGTTG AAGCCAAAGT TCAGTCAATT TCACTAGTCG GTGTGCCTAC GATGCGAGCA 
GATTGGGTCA CAGAATGCTG GAAACATCGT GATGACTCGT ATTTTGATGT GATGGAACCA 
TGTTTTGTAG ATAAGCATAG ACTTGGCGTA TTTGAAGGAC TCAGCCTATT CTTCCACGGC 
TTCAAACAAA CTGAGATAGA TGACATGCTC AGAAGCTTGG AGAATACAGG TGGAAAACTT 
GCTCCGAGTC CCACGTTGGC TACTCATGTT GTGTATAATC CTGATAATGA CGAAGTTGAG 
ACATTAGCAG TGTCTTCTAA TCAAGTTCAC GTCACTAAAG AGTGGTTCTG GGTAAGCCTG 
CACCGTGGTT GCTGTGCTAT AGAGGATAAC TTTGCTCTTC CAACTGGCAA ACTTCACAGA 
AAACCACTGG AAAGCAGTAG TGTCAGTAGC CCGATTGGAA AATCTCTTCG CTCCCAAAAT 
TCGAGATCTT TGTCATCAAT GCGTGACTGT AGTGTTGAAG GACCAACTTC GTTGAACATG 
ACACCAGACT ACATATACTC GAATGATGAT GTGGAGAACT CGTGCAAATC GCCACGTCAA 
CCATCGAAAC GGCTCAGAGT TTGTATGGAG ATGGTGGAGA CGGAGAAGAA CTACTTGTCA 
CTGTTGAAGC TTGTTGTCAA GTTCAAAGAA GCTCTGGAAT GTGAGATCTC ACACAACGAG 
TTCATGTCAA GATCCGATGT CGCCATGATG TTTGGAAAGC TGGATCCGAT TCTCGAACTT 
CACGAAAGGA TTTTCGCCAG ACTGACAGTA ATGTCAAACG AAGCGAATAC ACTTTTGACG 
GTCGTAAATG GAAAAAAGAA CGATGATAAG AATCTGGACT TTGCACAAGT GTGGATTGAT 
GCAAAAGAAG AAATGAAGAA GGCATATCCT CAGTACATCA ACTCTTATGA CACAATCAAA 
CGGTTATTCG ATAAACAGGA TCGAGAGAAT TCTAAATTTC ACACATTTTG CAAGGCAAAA 
GAGAGTAATC CAGAGTTTCA TCGGCTCAAG TTGACGGATT TGATGGTGAA ACCAGTGCAA 
CGTCTTCCAA GTGTCATTCT TTTACTCAAA GAAATGGCAA AGAAATCCGA TTCAAAGCTG 
AAGAACAATG CTGAAGAGGC CGCAAAAGCG ATTGACGAAG TTTTGAAAAC CGCCAACAAA 
ACTCGTGAAA AGAATGACAA CTTCATTTCC CATCTGAGCA AGTTCACTGA CATTGAAAAT 
GTTCCACCAA TTTTGGTGGC TGCCAATCGA ATGTTCATAC GAGAGTTGGT CGTTTCTCCG 
ATTGCAAGTA CGTCGCCAGG TCTACAGCAG TTTGCGAAAA TGAAATTATT TTTATTTCAT 
GACGTGCTAA TTATCACGAA AATACGCAGC GAGAAGAATA CAATGCAACG TCTAGCTCGT 
CACGCGTCGT TTGCCAGTTT ACATTCGAGA CAGCGTCGCC CCTACAGGTA CATTGACCAG 
ATACAGTTGA ACACGATGCG TAGTGCATTC CGAATTCGTG CTCCCGACGA GATTGCGAAG 
TGTCTGGAGT TGCAGCAGCA GAACGGAAGC ACAAAGCCAA ATGTGTTTGT ATGGTGCCTG 
ATTCATCGTG ACGAGCAAGG TGGTGACATT GACACAGTCT TCGAATCGAA TGATGATGAA 
GAGGTTCGAG ATTTTCTCGA CGATATTCAT GTGAAGATCT TGAGTGATTG TGGGAGATTC 
TTCTACTCGT CGGATCCATC AGATATGAAT GAGATCAACG ATGCGATGGT TTCGGATATG 
ACAACTCGAT ACTTTAGAAA TCTTTGCGGA GCGAAACCAC AATATGGACA CGGCAGTCAG 
ATGAACATTA CGGAATGGTC ACACAATGAG TCGTTTATGC CACCACCACA GTCACAGAGT 
CGAATGCGAC GAGCGTTCAG TAATGCTCAA CTCACACTTG CCACCACGCT GGGCTTTGGC 
AGAAATCAGA GTCGAAATAA CTTGGGACAA ATTAACGAGA ACACGACATT TCTGCAGTCT 
CCCAGAGTTT CGTGTGATCC AGCAGCTCTC GCAAACATGA CGTCAGTCGG CGGATTGCCT 
GGATGCCAAA GCGAATCTGA ATCGACGCCA AAGCGAGGAA TTCGTTCTCG CCTCACCTCG 
ACAACGTTCC TCGGCGGCCG ACACAACATA CTCGGACGAA ACACGTCGAT GCGCCGTCAG 
GCTACCGGAT TTGCTCATTC GGAAAAGGAA CTACCTCGCT CAAAAACATC TTCATATCGT 
GTCACCGATA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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ect-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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