let-19 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-19
Entrez Gene ID	174402
Full Name	Mediator of RNA polymerase II transcription subunit 13
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063465.5	NP_495866.2	Mediator of RNA polymerase II transcription subunit 13
NM_063465.4	NP_495866.2	Mediator of RNA polymerase II transcription subunit 13

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LET-19 and DPY-22 in the Mediator complex repress the transcription of Wnt target genes and are required for asymmetric cell division.
Title: Components of the transcriptional Mediator complex are required for asymmetric cell division in C. elegans.

The following let-19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337551	NM_063465.5 Latest version!	Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCa337551	NM_063465.4	Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-19 ( NM_063465.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495866.2 Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), mRNA.

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CloneID	OCa337551
Clone ID Related Accession (Same CDS sequence)	NM_063465.5 , NM_063465.4
Accession Version	NM_063465.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Mediator of RNA polymerase II transcription subunit 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063465.4.

ATGTCCTCCG CCAAGGATAG CGACAAGGAT GCCTCACGCA AAATGAAGCT CCAGAAGCGT 
CCAGAGTGGA ACAACGGTGG CAGCTTAGAA GACTGTATCT CAAATGTTTA TGCACTACTT 
GAATTGCCAG GCATTAAATG GAAATGTTAC CGCACAAAAC CAAATGCTCC ACGTGGTGTT 
GCACTGACTG CTGATTTTGT ACTCAAAGCA TACAGTAAAT GTTTATTGGA CGGAATTCTT 
TGCACATGGA GACGTAAACC ATTACCCCCA ACTGATAACA AAGAAACTCC AAAACTGACG 
AATTTTTCAA ATGATGCTCC AAAAGAGCTC TGGGTATTTT GGTATGATGA TGAGCCAGCA 
TTGCTTCAGA AAAATTGTGA AGGATTGGAT AACGACGATG AATTATCATC AGCAAATCAA 
ATGAATATTG TGAGCTACGA AGTACGCACA ATTATCTTCA AAGCTTTGCA CGTTGTTTTG 
GAAAGAGATC TTACAAAAGA CGGATTCGTT CGATTTGGAA GATGGTTTAC AGTTCCATAT 
GATGCACGAG AAAATTATCT ACATTTTACG TATCCATCAC ATTCTCCAGC AATTCGATTC 
AACTTTTTTG TTCATGGATC CAGCACGATA TGTGCTTCTG TTCAAGCACA AAGGCAACCA 
ACTTTGATAA CTTTGGCTAG ACGACATTTG GAGTGCAAAA CAAAACGTGT TGTGGTTGTA 
GTAGGTCCAT GGTCCATGCG CGGTCAACTT GTTCAAGACC AAATAGCTCT TCTTGCCGAC 
CCAAAAATTC AAGAATCAGC AGAAAAAGAA TGGAATCAAT GGAAAGATTA TTTGCAAATG 
GAAGAAAAAG AACCAGAAAA TGTGTCAGAA GAAAGGCAAA AATCTCCAGA TTCGGATCCA 
CCAGCTCCAC CACAAACAAC TCGAGTGTTA CTTGCAGATT CATCTGATGA AGAGCAATCA 
CAGAGTTTTG AGGCAATTGA TGAGTCAAAA AAAGACGATA TTCCACAGAG ATTACGATGG 
ATGTACGAGC CTGATTACCG TCAAGAAAAA TCAAAAGAAG AGACACCAGA AGAAAAAGAA 
AAAAGAGAAC AATCAAGAGA AGTGAGGAAA CAAAGAAGGA AATTAAGAGA AGAACGACGA 
AAAGAGGTTG AAAGGCAACG TACAGAAGTT AAAAATCCTG ATGATTATGA TTCGGATGTA 
GTGACAGATG AAGATGTTGG AGAGAAGGAA GAATGTGTGA ATAATGATGT GCCGAAAATG 
GTTCTTCTTG ATATTGACGG TGTTCGATTA TTATACCCAT CGAAATTCCT TTGTGTCACA 
ATTGATGAGG ATCGGCAAAT GCTTGAGTCA ATTGGTCTGA AGACAAATCA ACAACCACCT 
GAATTACAAG CCCAAAATCG TCGCCCACGT AACAAACTTG TTGTCAACGT TGCGAATCCA 
ATTCTATCTA CAATGGCAGT CTACCAATAC TGTGAAAATG AACGGTTAAT TTCAAAAGAA 
AATGTTCCAC AAAAAAGTGC CACTAGTGGG CAGCTCTTGC CCCCACTAAA AGAAAAATTC 
TTAAATTGGA AGTTTACTGC AAAAAAGTAT GGATGCAGAG GAGATGTATC ATGTCGACAT 
TGTGCTGAAG CTGAATACCA AGGAGAATCC GATGGACAAG TTATACTAGA CGTTGATCGT 
CACAATAGAT TTCTAAAATA TGGTCCATAT ACGGAAAATT CGATTAGATG GCACAAAATT 
CGGAGTCGAA TTCAAAAGAA ATTTGATTTT CATGCCTATC ACAAGAAGCA TCCAATTGGG 
CCAATTACAC CTAGAAAGGC TGGAGACAGT GTGAAACAGA CAAAACAACT GAGTGCGGTC 
AAACGAAGAA GGAGAGCTCG TCGAGAAAGA GCACAAAAGA AGAGGCGTGA CAAAAAACGT 
CCAACTGGAT CAACTCGAGC TGATAGCGAA GATGATTCAT TTCGAATGGA CAAACTTTCT 
CAGGATGATT GGGCTAATTT TGAGGGAGAG CACCCAAAGG GAATCCCTGA AATTGAAGTT 
GATAAGGATT TTGAACTTCA TAATGACATC AAAATTCCCA AAGTTCCAAA TGATGACAAC 
TACAAGCCAA AATCGTTTTT GCCAATTACA TATCTCACCA ACAATGTAGA TAATAATGAC 
TTTGAGTCAA TGCCTGGAGA TGATGATATG TCTGAAGAGC GTTTCAATCA GATATTCAAA 
GACAAATACA ATCGATGGAA GCCTGGAATG TCGAGAGCAG AAAAAATCGA GCACGCTCAT 
TATCAATTTG ATCCATCAAA TCAAATGACC GGTTCACCAC GAGAATACCA TTTGAAGGAT 
TTGCCAAAGA AGATTCAAAA ATATGAATCA ATACTTCCTA CACCAAAAGG AAATAAGTTT 
TTCAAAGGAA GCATTTGTCG AAAAGGATTA AGAGGTTATG ATGAAATTTG TGCGGATCAA 
GATGAAAAAG AAATGATGAG AAAAGCAAAA TGGTTGAAGA AAGCAAGAAG GAAAGTTAGC 
AAACAACAGA TAAAGAAGGC GGGGCAGTAT CATATTGATA TGCAGAAACA AATAGTCAAA 
CGTTCATTGA AAAGAGCCAT CAGGAAATTA AAACTAACTA CCAAAAAGGA TTCACAATCG 
AAAGGAACAA AACAGCAGAA ACTCGTTCTA TTATCATATA AAAAGAAGAA AGTCGACGAG 
AAAAAGTTCC AACAATACCT GAAAGAAGTG GAAAATGTTT TAGACAAACT TGATGTAAAC 
TCATTTTGTA AGAAAAATGA GAAAGCTGGA TTTTCGGAGA AAGCAATACC AAGTGGATAT 
ATTATCCCTG ACTATGAATC GTCAATTCAC TGCGGGATGG AAGAAGAATC TGATTCTGAG 
ATAGACGAGC CACGCCATGG ACTTGGAGAA GGAACTTGTG GGCAATATCT GGAGGGAGAA 
TCTGTGGATC TTGCTGACAT GGAACATCAT CATAACGAAT TGCCTATGGA AGTGACCAGT 
ATCGGACATA TCAGAACAGT CGGCGAGCAT ATTGGTCCAG ATGGAATGTT ATCGCCACCT 
GCCAGCAACG AAATGCCAAA AGGAGGACCT CTATCGGTGG GTCCACCATC CATCGAGTCG 
CAAGGACTCA ATCAGATCTA CCCGACGCCG CCATCAGTTC AGATGCTCCT GGGAGATGCA 
GCTCAAGCAC ATTCTCCTGT TATGCATACA AAGTCACGTT ACGCGATGAA TACGTTGGAA 
GATGATGTTG AAAGAATCGC TGTGCCTGGT GGAGTCGGAA GTCTTGTGGA AATCGAGGCT 
GACGATATTG AAACAAAGAA TATGCAAAAG TGGAGCAAAC TTTCAAATCA ATCAAAACTG 
TCGAAGTTCA TTGCATTGTC TTGCAAAGAT CCGTTCCTGA AATCGAAGAA GAAGAAATAT 
GATACACCTG TCACTAGCAA GTTTAAGATT GAAAATGAAC CTCCAAGTAT GAGCGCAGAG 
CTGGTGTACG CACCGATGGT CACTCGCAAC TTCTACTCGG CGCCGCATCG AACATTTGGT 
ACCTTGGCTC AGAAAGTGGT TGACAAACGA CCTTCTCAAT TTGCAATCCC TTCAACATCA 
ACAGCAGCTC ATCCTGGATT GACTACACCT ATGGCATCAA TTTTCCAATC ACCCACAATG 
CCACCTCCAC ATACCCCATC ACCTTTTTCT GCTGGTCCAA TGAGTCATCA TCCAATGGGA 
CCACCTGCGT ATCCAGGAAC TCCTGGCTCA TTTCCACCAA CAACGCCAAC ATATCCTGGA 
ATGACACCAC GACCAGGGCC AAACTTTGGA CCTCCTGGTC CAGTCTATCC AAACTCTCAA 
CAAATGATGG GCCCGGGTGG ATATCCACCA CAAATGGGTA TGATAAACTC TCAACGGCAT 
TTCCAAAATC AGCAGATGTA TCAACAGCAA ATGGTGAGAA TGCAGCAAAT GCGCCAGATG 
GGTGGACCTC CAGGGTATGG TGCACCTGGT GTATCTCCTT ATCAACAGGT TAACGCAATT 
ACAAGTCCAG CAGGATTTGG AATGCCACCC AACGGTCCGT CTTCTGTTCA AAGGCTTAAC 
AATTTTGTTA ATCAATCACA ATTCGGCCCG AACAATGGTC ATCCAACACC ACCGTACGGT 
TATATGCAAC AAATGATTCC ACAATCACCA AACTTCAATC AACAGTACAA TGGAATGGGA 
AATCAACTAA TGAGTCCAAT ACATCAACAC CAATTCCATC AGCAACAAAT GGTTCAGCAG 
CAGCAGCAAC AACAAATGCA GATGCATAGA CAACAGATGC AGATGCAAAA TCAAAATATG 
TCCCAACCGT TCCAACCACC AACTGCTCAA ATGTTACAAA TGCAGCAAAA TGAGCAAATG 
AGAATAGCCT TTTCGACATT GTCAGAACGT GATAAGGCTA AATTCCAAAA ACGACAAGGT 
CAACTGAGGA GTGGAATTAT GCCCAAATCA CTACAGCCAG CAGTTCTAAA GAATCTGAAA 
AATCCACCTG ATGCTAACAT TCCATATATG AAACAGGATG CATTATTTCG ACCCCCAGAA 
TCGAATTCAC TTCAAGGAGA GAGTCTTACA ATTGCAATTG TTCTCTCGGA TACACTTCTT 
GATTTACATT TTGATTCTGT GTTTGATGCT TGCCCAATCT GTTCTTGTTC AGTTTCAATT 
CGTTCTCGAG ATATTGGAAT GTACATCACT CCACATACAG TCCTCTACTC CACAGAACGT 
GGACATACAA ATATGAGAGA ACACACTACT GGACCATGGA GTGGTTTCAA TGTCAATTCG 
GCAACTAATT GTACTTGTGG ATTTAGTGCA ATTCGACACC GCTATCTGTC TTGCTGTTCT 
GGCCTTTTTG AAGAAGATGC TGATGAAGCA ACAGCTTCAG AGCATGCAGT GGCACCAGTC 
ATTCCTCCTT TGGATTATCC GAAAAAATCA ACTGCACGGG ATATGTCTTG GTTCGATTCA 
AAATCAGTTC ACGACCTGGC TCTTCTTGAT CAAATCCGAC AAATGGCTTT CTCGAACAGT 
CTTGGAAAAG CGGTTTCACA TATGGCAACG ATCAAAGAAC ACAATAGAAA TCTTGCGATT 
GCTGTTGATC TTGGCAATGA TGTTACTGTT CCCTCCGAAT ATATCCTTTC TCATGTCGAT 
GCTTTGGAAC TTCTGATGTT GGGAAATTCG GTTCTGGGTC CAATGAAAAA CACAAGTATT 
GCTAATCAAG TTGCATCTCA CAATAAGTTC CTTGGATTTT TCCATCCATG GGGAATTCAA 
ATTGCAAATG GAATGACTGA ACTTGAAGCA TCGGAATGGG TTGACTTGCT TGGCGTTGTA 
ACCCCAACAT TGGAAGGTTC AATGAAACAA GCAAGACACA TACCTGCAGA AACTCCATTT 
GAACTTGATG GTCCAATGAC GTGGAAACAA ATTGTCACCA AGAGTAACAG AGGAAAACCT 
CCAGCAGATG ATGAAGAAGA TTTTTCTCTA CCTGAACCAG TTCCTGCAAT AATGAGAGCA 
TTGACAAAGG AAGCAATTCG AGCTGCTCCA AACATTGAAC AATACTACGA ACAAGCAGGA 
TTAGGACCAG TGGATCAGCC AAAAGATGTC ATGTACATTA CAGTTATTCC AGACGATGAT 
GACATTTATT CACGAACTGT CGAATTCATG GATACTTTGA CACGTACTTA TGAAAAAATG 
AGACTAGGAC GTCATATTCC ATTCCCTGTC TCAACTGGTA CAGCAACTCG TTTCCGCGAA 
GCATTCAAGT CATGTCAATC TCAATTCCCA TTCCAAGAGC AACAACACTT GGCATACACA 
AATCAAACAC TTCGAAATAT GCCTCAAACA TTGGCTGATA CTCCAGACCC GGTGAACAGC 
GATCCAAAAG TTTGGTCGAG GCGAGCGAAG AGAAAGTTGG ATTTTGAAGA AGAACTGAAA 
CTCCCTGAAG CTGGCTCAGA AACTATTGAT GAATCCGATG ATGACGAAGA AAACGAAGAA 
GTTTCAAAAG AAGAGAACAA CGAGCTCATT GATTCAGAAG ACGTTATGGA CGAGGTTCCA 
AAAGGTTTCT ACGAACGGGA AGGAATTTTG CGTGTTGGGG CTCCAATGGA GCCGTCAAGA 
AGTCCGCATA CTGTAACAAA CACTCCTGAA TTTGACAACA TGACAAAATA TCTGAGTGAT 
GAGAATGGAT TTGTGAGCAG ACTTCGGCTT TATTTACAAC AAATGGAGGA TCTAGTTCAT 
TTTGCATTGA GTGAGAATCC AGAAGCGTTT GATAGAAGAG GGTATCGATA TCAATTAGCA 
GTTGAAGGAA GACTCAAGAG ACAGAAACAT AAAATGGATA TCATAGAAGA ACGTCTCAGA 
TTTGAAGCTT CGAAAGACTT TGATGAACAA AGTGATCGTA AGGAACCTCT TGAAGAAAAT 
GATCCTGAGA TAACTCCTGG AATAGTAATA AGTAACAATC CGGTTGAATC TTGGATTGAT 
GATGAACTAG AAGAGAAACG TCAGCAGCGG CAGAAGGAAA ATGAACAGTA CCCACCAGAA 
GCTCAACAAG CACCATCTCC AGTACCTGCG GGAATGATTC ATATTCCTGA AACATTATCC 
AAAGAGGAAA GAGCCGTTCA ACCAACTCTT GAAATGATCT TAGCCGATCC AGCAACTGTC 
GCTCCTATCA ATGCTCAAAA TATTGTTTGG AAACAGCGCG ACACTCGAGT TCCAAACCCA 
TTCCCCTCAT CGAATCAGCC ACCTGTCGCA TTTGAAGCTG CTGGATCTCC AGACACGGAT 
GCTTATTCAA CCCTTCCACA CGTTATCGTC GTCTACGTGG TTAATCCGTT TTCATACGGA 
CCAGAAGGAC ATTCAGCATT GCATATGAGA ATAGCCATTC TTGCATTCAT TCGGGCATTC 
AATTCGATTA TGTGCAAAAT TCCTTACGAA AAACGTCCTC AACTTCAGCT GGAAATCGTT 
GGAATGGAAG GCATGGATAA TGTTGCAAAG CCGATTCCAG ATTACTTCAA TGATGCCAAG 
ATTCCATTCG ATTTACTCAA TGATCGTCCG ATTCGAGTCG AACGACCTGG AGAGTCTGTT 
CAAGGAGAGC TAGCTCGGTC TTTATCAATT GCTGTATACA CCCATCCACG TGTATTCTTC 
CCGGATGTCT ACAAGTCTGC TTCAGCAAGA TGTATGACAG CATTTGGTCC TGGAAGTCAG 
CTGATGAATA CAATCAACAA AATTGAGGCG CTAAACAAGG ACTCATTTGC TCGAATGGCA 
AAACGATCAA AGACTACACT GGACACCATG GACATGTACC GACATCCAGG AATGATTCAA 
GCACAGCAGT CGACAGAAAA GAAAAACTAT ATTGCTTATC GAGTTCCATC GAACATCGCT 
GTTCTTGCAC CTCCACCAAT GGTATATCAA ATGGATGAAA AAGGAAAAGC GATAATGAAT 
CAACTGGATG AGCAAACTCT TTTCATCTCA TATTGCCTTG TTGGTACTGA TTTCCTTGTA 
GCTACAGCAA CTGATGCTCA AGGAAAACTG ATTGATAACT GTATTTCGAA TATCAAACCA 
AGACGCCAGA GCAATCAGGT GTATCGATAC AGAAATAAGA CACAAATCCT AGATGGAATG 
GGAAAACTTT GGTCATTTAT TCTTGGAATC ATGGCTTCGG AAACTAAAAA TTGGAGACTT 
GTTGTTGGAA GACTCGGACG CATCGGACAT GGAGAGTTCA GAGCCTGGAC TCATCTTCTC 
AATAAAACTT CTCTTCTTCG TTATTCGGGA AGTTTGAAGG ATATTTGTGG TGCCTGTAGA 
AGCATGCCTT CAGTCATTGG AACTCCAGCT ATTCTCTCAG CATGTCTCAT TACTCTTGAG 
CCTGAACCAA GTATTCGAAT CATGCCAGAG TTCCATGATC AGGAATTGTC AACAAAGAAG 
AGCTTTCTAT TCCAAACTCC TGGAGATTTA TCATGCACTC ACATTCTCAC TTTCCCAGTT 
GGAACCGAGA TTAACCTAGA AGTTCAAGAC CAAACTGCGG ATACCAAAGC AGATGAAAAT 
TGGGAGTTCG GAGATCTTGA CATCATGGAA GGTCTTGATG ATGGAGATAC TGAAATCATG 
AAAGATCTTG GACTGGAAAC TCCAAGCAGT GCTGCAATTC GACAGACTGG CGGACCTTCA 
ATGTTTTTCA GCGAAGATTC GTCTTCCATT GAAATTCAAA ATCAACCTCT TGCATCTGGA 
TACTACATCT CCACAGCACC GGCTCCCGAA CTTCCAGCAT GGTTCTGGGC AACATGTCCA 
TCTGCAAAAC GTCATTCTCC TGTTCATCTG AAATCCTCGT TACATATTCA TATCAGTGAA 
GTAAAGAACG ACGATATTGC AATGGAATCT ACAAAAGAAA AAGAAAAGGA CAAGGAAAAA 
GATGAAAAAG ATGCTCATCC ATTGGAATCT CGACAAACTG AGGAAGTGCT TCGACATGTA 
CTCGAGTCGT ATAATGCTCT CTCGTGGCTC AATCTCAATC GACAAACTGG TGACCGTTAC 
AGTTGTCTGC CAATTCACAT TCAACATCTT CTGAGACTCT ACCACAGTGT GGCTCGTCTA 
CTTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495866.2
CDS	103..8691
Translation	MSSAKDSDKDASRKMKLQKRPEWNNGGSLEDCISNVYALLELPGIKWKCYRTKPNAPRGVALTADFVLKAYSKCLLDGILCTWRRKPLPPTDNKETPKLTNFSNDAPKELWVFWYDDEPALLQKNCEGLDNDDELSSANQMNIVSYEVRTIIFKALHVVLERDLTKDGFVRFGRWFTVPYDARENYLHFTYPSHSPAIRFNFFVHGSSTICASVQAQRQPTLITLARRHLECKTKRVVVVVGPWSMRGQLVQDQIALLADPKIQESAEKEWNQWKDYLQMEEKEPENVSEERQKSPDSDPPAPPQTTRVLLADSSDEEQSQSFEAIDESKKDDIPQRLRWMYEPDYRQEKSKEETPEEKEKREQSREVRKQRRKLREERRKEVERQRTEVKNPDDYDSDVVTDEDVGEKEECVNNDVPKMVLLDIDGVRLLYPSKFLCVTIDEDRQMLESIGLKTNQQPPELQAQNRRPRNKLVVNVANPILSTMAVYQYCENERLISKENVPQKSATSGQLLPPLKEKFLNWKFTAKKYGCRGDVSCRHCAEAEYQGESDGQVILDVDRHNRFLKYGPYTENSIRWHKIRSRIQKKFDFHAYHKKHPIGPITPRKAGDSVKQTKQLSAVKRRRRARRERAQKKRRDKKRPTGSTRADSEDDSFRMDKLSQDDWANFEGEHPKGIPEIEVDKDFELHNDIKIPKVPNDDNYKPKSFLPITYLTNNVDNNDFESMPGDDDMSEERFNQIFKDKYNRWKPGMSRAEKIEHAHYQFDPSNQMTGSPREYHLKDLPKKIQKYESILPTPKGNKFFKGSICRKGLRGYDEICADQDEKEMMRKAKWLKKARRKVSKQQIKKAGQYHIDMQKQIVKRSLKRAIRKLKLTTKKDSQSKGTKQQKLVLLSYKKKKVDEKKFQQYLKEVENVLDKLDVNSFCKKNEKAGFSEKAIPSGYIIPDYESSIHCGMEEESDSEIDEPRHGLGEGTCGQYLEGESVDLADMEHHHNELPMEVTSIGHIRTVGEHIGPDGMLSPPASNEMPKGGPLSVGPPSIESQGLNQIYPTPPSVQMLLGDAAQAHSPVMHTKSRYAMNTLEDDVERIAVPGGVGSLVEIEADDIETKNMQKWSKLSNQSKLSKFIALSCKDPFLKSKKKKYDTPVTSKFKIENEPPSMSAELVYAPMVTRNFYSAPHRTFGTLAQKVVDKRPSQFAIPSTSTAAHPGLTTPMASIFQSPTMPPPHTPSPFSAGPMSHHPMGPPAYPGTPGSFPPTTPTYPGMTPRPGPNFGPPGPVYPNSQQMMGPGGYPPQMGMINSQRHFQNQQMYQQQMVRMQQMRQMGGPPGYGAPGVSPYQQVNAITSPAGFGMPPNGPSSVQRLNNFVNQSQFGPNNGHPTPPYGYMQQMIPQSPNFNQQYNGMGNQLMSPIHQHQFHQQQMVQQQQQQQMQMHRQQMQMQNQNMSQPFQPPTAQMLQMQQNEQMRIAFSTLSERDKAKFQKRQGQLRSGIMPKSLQPAVLKNLKNPPDANIPYMKQDALFRPPESNSLQGESLTIAIVLSDTLLDLHFDSVFDACPICSCSVSIRSRDIGMYITPHTVLYSTERGHTNMREHTTGPWSGFNVNSATNCTCGFSAIRHRYLSCCSGLFEEDADEATASEHAVAPVIPPLDYPKKSTARDMSWFDSKSVHDLALLDQIRQMAFSNSLGKAVSHMATIKEHNRNLAIAVDLGNDVTVPSEYILSHVDALELLMLGNSVLGPMKNTSIANQVASHNKFLGFFHPWGIQIANGMTELEASEWVDLLGVVTPTLEGSMKQARHIPAETPFELDGPMTWKQIVTKSNRGKPPADDEEDFSLPEPVPAIMRALTKEAIRAAPNIEQYYEQAGLGPVDQPKDVMYITVIPDDDDIYSRTVEFMDTLTRTYEKMRLGRHIPFPVSTGTATRFREAFKSCQSQFPFQEQQHLAYTNQTLRNMPQTLADTPDPVNSDPKVWSRRAKRKLDFEEELKLPEAGSETIDESDDDEENEEVSKEENNELIDSEDVMDEVPKGFYEREGILRVGAPMEPSRSPHTVTNTPEFDNMTKYLSDENGFVSRLRLYLQQMEDLVHFALSENPEAFDRRGYRYQLAVEGRLKRQKHKMDIIEERLRFEASKDFDEQSDRKEPLEENDPEITPGIVISNNPVESWIDDELEEKRQQRQKENEQYPPEAQQAPSPVPAGMIHIPETLSKEERAVQPTLEMILADPATVAPINAQNIVWKQRDTRVPNPFPSSNQPPVAFEAAGSPDTDAYSTLPHVIVVYVVNPFSYGPEGHSALHMRIAILAFIRAFNSIMCKIPYEKRPQLQLEIVGMEGMDNVAKPIPDYFNDAKIPFDLLNDRPIRVERPGESVQGELARSLSIAVYTHPRVFFPDVYKSASARCMTAFGPGSQLMNTINKIEALNKDSFARMAKRSKTTLDTMDMYRHPGMIQAQQSTEKKNYIAYRVPSNIAVLAPPPMVYQMDEKGKAIMNQLDEQTLFISYCLVGTDFLVATATDAQGKLIDNCISNIKPRRQSNQVYRYRNKTQILDGMGKLWSFILGIMASETKNWRLVVGRLGRIGHGEFRAWTHLLNKTSLLRYSGSLKDICGACRSMPSVIGTPAILSACLITLEPEPSIRIMPEFHDQELSTKKSFLFQTPGDLSCTHILTFPVGTEINLEVQDQTADTKADENWEFGDLDIMEGLDDGDTEIMKDLGLETPSSAAIRQTGGPSMFFSEDSSSIEIQNQPLASGYYISTAPAPELPAWFWATCPSAKRHSPVHLKSSLHIHISEVKNDDIAMESTKEKEKDKEKDEKDAHPLESRQTEEVLRHVLESYNALSWLNLNRQTGDRYSCLPIHIQHLLRLYHSVARLLA

Target ORF information:

RefSeq Version	NM_063465.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063465.5

ORF Insert Sequence:

ATGTCCTCCG CCAAGGATAG CGACAAGGAT GCCTCACGCA AAATGAAGCT CCAGAAGCGT 
CCAGAGTGGA ACAACGGTGG CAGCTTAGAA GACTGTATCT CAAATGTTTA TGCACTACTT 
GAATTGCCAG GCATTAAATG GAAATGTTAC CGCACAAAAC CAAATGCTCC ACGTGGTGTT 
GCACTGACTG CTGATTTTGT ACTCAAAGCA TACAGTAAAT GTTTATTGGA CGGAATTCTT 
TGCACATGGA GACGTAAACC ATTACCCCCA ACTGATAACA AAGAAACTCC AAAACTGACG 
AATTTTTCAA ATGATGCTCC AAAAGAGCTC TGGGTATTTT GGTATGATGA TGAGCCAGCA 
TTGCTTCAGA AAAATTGTGA AGGATTGGAT AACGACGATG AATTATCATC AGCAAATCAA 
ATGAATATTG TGAGCTACGA AGTACGCACA ATTATCTTCA AAGCTTTGCA CGTTGTTTTG 
GAAAGAGATC TTACAAAAGA CGGATTCGTT CGATTTGGAA GATGGTTTAC AGTTCCATAT 
GATGCACGAG AAAATTATCT ACATTTTACG TATCCATCAC ATTCTCCAGC AATTCGATTC 
AACTTTTTTG TTCATGGATC CAGCACGATA TGTGCTTCTG TTCAAGCACA AAGGCAACCA 
ACTTTGATAA CTTTGGCTAG ACGACATTTG GAGTGCAAAA CAAAACGTGT TGTGGTTGTA 
GTAGGTCCAT GGTCCATGCG CGGTCAACTT GTTCAAGACC AAATAGCTCT TCTTGCCGAC 
CCAAAAATTC AAGAATCAGC AGAAAAAGAA TGGAATCAAT GGAAAGATTA TTTGCAAATG 
GAAGAAAAAG AACCAGAAAA TGTGTCAGAA GAAAGGCAAA AATCTCCAGA TTCGGATCCA 
CCAGCTCCAC CACAAACAAC TCGAGTGTTA CTTGCAGATT CATCTGATGA AGAGCAATCA 
CAGAGTTTTG AGGCAATTGA TGAGTCAAAA AAAGACGATA TTCCACAGAG ATTACGATGG 
ATGTACGAGC CTGATTACCG TCAAGAAAAA TCAAAAGAAG AGACACCAGA AGAAAAAGAA 
AAAAGAGAAC AATCAAGAGA AGTGAGGAAA CAAAGAAGGA AATTAAGAGA AGAACGACGA 
AAAGAGGTTG AAAGGCAACG TACAGAAGTT AAAAATCCTG ATGATTATGA TTCGGATGTA 
GTGACAGATG AAGATGTTGG AGAGAAGGAA GAATGTGTGA ATAATGATGT GCCGAAAATG 
GTTCTTCTTG ATATTGACGG TGTTCGATTA TTATACCCAT CGAAATTCCT TTGTGTCACA 
ATTGATGAGG ATCGGCAAAT GCTTGAGTCA ATTGGTCTGA AGACAAATCA ACAACCACCT 
GAATTACAAG CCCAAAATCG TCGCCCACGT AACAAACTTG TTGTCAACGT TGCGAATCCA 
ATTCTATCTA CAATGGCAGT CTACCAATAC TGTGAAAATG AACGGTTAAT TTCAAAAGAA 
AATGTTCCAC AAAAAAGTGC CACTAGTGGG CAGCTCTTGC CCCCACTAAA AGAAAAATTC 
TTAAATTGGA AGTTTACTGC AAAAAAGTAT GGATGCAGAG GAGATGTATC ATGTCGACAT 
TGTGCTGAAG CTGAATACCA AGGAGAATCC GATGGACAAG TTATACTAGA CGTTGATCGT 
CACAATAGAT TTCTAAAATA TGGTCCATAT ACGGAAAATT CGATTAGATG GCACAAAATT 
CGGAGTCGAA TTCAAAAGAA ATTTGATTTT CATGCCTATC ACAAGAAGCA TCCAATTGGG 
CCAATTACAC CTAGAAAGGC TGGAGACAGT GTGAAACAGA CAAAACAACT GAGTGCGGTC 
AAACGAAGAA GGAGAGCTCG TCGAGAAAGA GCACAAAAGA AGAGGCGTGA CAAAAAACGT 
CCAACTGGAT CAACTCGAGC TGATAGCGAA GATGATTCAT TTCGAATGGA CAAACTTTCT 
CAGGATGATT GGGCTAATTT TGAGGGAGAG CACCCAAAGG GAATCCCTGA AATTGAAGTT 
GATAAGGATT TTGAACTTCA TAATGACATC AAAATTCCCA AAGTTCCAAA TGATGACAAC 
TACAAGCCAA AATCGTTTTT GCCAATTACA TATCTCACCA ACAATGTAGA TAATAATGAC 
TTTGAGTCAA TGCCTGGAGA TGATGATATG TCTGAAGAGC GTTTCAATCA GATATTCAAA 
GACAAATACA ATCGATGGAA GCCTGGAATG TCGAGAGCAG AAAAAATCGA GCACGCTCAT 
TATCAATTTG ATCCATCAAA TCAAATGACC GGTTCACCAC GAGAATACCA TTTGAAGGAT 
TTGCCAAAGA AGATTCAAAA ATATGAATCA ATACTTCCTA CACCAAAAGG AAATAAGTTT 
TTCAAAGGAA GCATTTGTCG AAAAGGATTA AGAGGTTATG ATGAAATTTG TGCGGATCAA 
GATGAAAAAG AAATGATGAG AAAAGCAAAA TGGTTGAAGA AAGCAAGAAG GAAAGTTAGC 
AAACAACAGA TAAAGAAGGC GGGGCAGTAT CATATTGATA TGCAGAAACA AATAGTCAAA 
CGTTCATTGA AAAGAGCCAT CAGGAAATTA AAACTAACTA CCAAAAAGGA TTCACAATCG 
AAAGGAACAA AACAGCAGAA ACTCGTTCTA TTATCATATA AAAAGAAGAA AGTCGACGAG 
AAAAAGTTCC AACAATACCT GAAAGAAGTG GAAAATGTTT TAGACAAACT TGATGTAAAC 
TCATTTTGTA AGAAAAATGA GAAAGCTGGA TTTTCGGAGA AAGCAATACC AAGTGGATAT 
ATTATCCCTG ACTATGAATC GTCAATTCAC TGCGGGATGG AAGAAGAATC TGATTCTGAG 
ATAGACGAGC CACGCCATGG ACTTGGAGAA GGAACTTGTG GGCAATATCT GGAGGGAGAA 
TCTGTGGATC TTGCTGACAT GGAACATCAT CATAACGAAT TGCCTATGGA AGTGACCAGT 
ATCGGACATA TCAGAACAGT CGGCGAGCAT ATTGGTCCAG ATGGAATGTT ATCGCCACCT 
GCCAGCAACG AAATGCCAAA AGGAGGACCT CTATCGGTGG GTCCACCATC CATCGAGTCG 
CAAGGACTCA ATCAGATCTA CCCGACGCCG CCATCAGTTC AGATGCTCCT GGGAGATGCA 
GCTCAAGCAC ATTCTCCTGT TATGCATACA AAGTCACGTT ACGCGATGAA TACGTTGGAA 
GATGATGTTG AAAGAATCGC TGTGCCTGGT GGAGTCGGAA GTCTTGTGGA AATCGAGGCT 
GACGATATTG AAACAAAGAA TATGCAAAAG TGGAGCAAAC TTTCAAATCA ATCAAAACTG 
TCGAAGTTCA TTGCATTGTC TTGCAAAGAT CCGTTCCTGA AATCGAAGAA GAAGAAATAT 
GATACACCTG TCACTAGCAA GTTTAAGATT GAAAATGAAC CTCCAAGTAT GAGCGCAGAG 
CTGGTGTACG CACCGATGGT CACTCGCAAC TTCTACTCGG CGCCGCATCG AACATTTGGT 
ACCTTGGCTC AGAAAGTGGT TGACAAACGA CCTTCTCAAT TTGCAATCCC TTCAACATCA 
ACAGCAGCTC ATCCTGGATT GACTACACCT ATGGCATCAA TTTTCCAATC ACCCACAATG 
CCACCTCCAC ATACCCCATC ACCTTTTTCT GCTGGTCCAA TGAGTCATCA TCCAATGGGA 
CCACCTGCGT ATCCAGGAAC TCCTGGCTCA TTTCCACCAA CAACGCCAAC ATATCCTGGA 
ATGACACCAC GACCAGGGCC AAACTTTGGA CCTCCTGGTC CAGTCTATCC AAACTCTCAA 
CAAATGATGG GCCCGGGTGG ATATCCACCA CAAATGGGTA TGATAAACTC TCAACGGCAT 
TTCCAAAATC AGCAGATGTA TCAACAGCAA ATGGTGAGAA TGCAGCAAAT GCGCCAGATG 
GGTGGACCTC CAGGGTATGG TGCACCTGGT GTATCTCCTT ATCAACAGGT TAACGCAATT 
ACAAGTCCAG CAGGATTTGG AATGCCACCC AACGGTCCGT CTTCTGTTCA AAGGCTTAAC 
AATTTTGTTA ATCAATCACA ATTCGGCCCG AACAATGGTC ATCCAACACC ACCGTACGGT 
TATATGCAAC AAATGATTCC ACAATCACCA AACTTCAATC AACAGTACAA TGGAATGGGA 
AATCAACTAA TGAGTCCAAT ACATCAACAC CAATTCCATC AGCAACAAAT GGTTCAGCAG 
CAGCAGCAAC AACAAATGCA GATGCATAGA CAACAGATGC AGATGCAAAA TCAAAATATG 
TCCCAACCGT TCCAACCACC AACTGCTCAA ATGTTACAAA TGCAGCAAAA TGAGCAAATG 
AGAATAGCCT TTTCGACATT GTCAGAACGT GATAAGGCTA AATTCCAAAA ACGACAAGGT 
CAACTGAGGA GTGGAATTAT GCCCAAATCA CTACAGCCAG CAGTTCTAAA GAATCTGAAA 
AATCCACCTG ATGCTAACAT TCCATATATG AAACAGGATG CATTATTTCG ACCCCCAGAA 
TCGAATTCAC TTCAAGGAGA GAGTCTTACA ATTGCAATTG TTCTCTCGGA TACACTTCTT 
GATTTACATT TTGATTCTGT GTTTGATGCT TGCCCAATCT GTTCTTGTTC AGTTTCAATT 
CGTTCTCGAG ATATTGGAAT GTACATCACT CCACATACAG TCCTCTACTC CACAGAACGT 
GGACATACAA ATATGAGAGA ACACACTACT GGACCATGGA GTGGTTTCAA TGTCAATTCG 
GCAACTAATT GTACTTGTGG ATTTAGTGCA ATTCGACACC GCTATCTGTC TTGCTGTTCT 
GGCCTTTTTG AAGAAGATGC TGATGAAGCA ACAGCTTCAG AGCATGCAGT GGCACCAGTC 
ATTCCTCCTT TGGATTATCC GAAAAAATCA ACTGCACGGG ATATGTCTTG GTTCGATTCA 
AAATCAGTTC ACGACCTGGC TCTTCTTGAT CAAATCCGAC AAATGGCTTT CTCGAACAGT 
CTTGGAAAAG CGGTTTCACA TATGGCAACG ATCAAAGAAC ACAATAGAAA TCTTGCGATT 
GCTGTTGATC TTGGCAATGA TGTTACTGTT CCCTCCGAAT ATATCCTTTC TCATGTCGAT 
GCTTTGGAAC TTCTGATGTT GGGAAATTCG GTTCTGGGTC CAATGAAAAA CACAAGTATT 
GCTAATCAAG TTGCATCTCA CAATAAGTTC CTTGGATTTT TCCATCCATG GGGAATTCAA 
ATTGCAAATG GAATGACTGA ACTTGAAGCA TCGGAATGGG TTGACTTGCT TGGCGTTGTA 
ACCCCAACAT TGGAAGGTTC AATGAAACAA GCAAGACACA TACCTGCAGA AACTCCATTT 
GAACTTGATG GTCCAATGAC GTGGAAACAA ATTGTCACCA AGAGTAACAG AGGAAAACCT 
CCAGCAGATG ATGAAGAAGA TTTTTCTCTA CCTGAACCAG TTCCTGCAAT AATGAGAGCA 
TTGACAAAGG AAGCAATTCG AGCTGCTCCA AACATTGAAC AATACTACGA ACAAGCAGGA 
TTAGGACCAG TGGATCAGCC AAAAGATGTC ATGTACATTA CAGTTATTCC AGACGATGAT 
GACATTTATT CACGAACTGT CGAATTCATG GATACTTTGA CACGTACTTA TGAAAAAATG 
AGACTAGGAC GTCATATTCC ATTCCCTGTC TCAACTGGTA CAGCAACTCG TTTCCGCGAA 
GCATTCAAGT CATGTCAATC TCAATTCCCA TTCCAAGAGC AACAACACTT GGCATACACA 
AATCAAACAC TTCGAAATAT GCCTCAAACA TTGGCTGATA CTCCAGACCC GGTGAACAGC 
GATCCAAAAG TTTGGTCGAG GCGAGCGAAG AGAAAGTTGG ATTTTGAAGA AGAACTGAAA 
CTCCCTGAAG CTGGCTCAGA AACTATTGAT GAATCCGATG ATGACGAAGA AAACGAAGAA 
GTTTCAAAAG AAGAGAACAA CGAGCTCATT GATTCAGAAG ACGTTATGGA CGAGGTTCCA 
AAAGGTTTCT ACGAACGGGA AGGAATTTTG CGTGTTGGGG CTCCAATGGA GCCGTCAAGA 
AGTCCGCATA CTGTAACAAA CACTCCTGAA TTTGACAACA TGACAAAATA TCTGAGTGAT 
GAGAATGGAT TTGTGAGCAG ACTTCGGCTT TATTTACAAC AAATGGAGGA TCTAGTTCAT 
TTTGCATTGA GTGAGAATCC AGAAGCGTTT GATAGAAGAG GGTATCGATA TCAATTAGCA 
GTTGAAGGAA GACTCAAGAG ACAGAAACAT AAAATGGATA TCATAGAAGA ACGTCTCAGA 
TTTGAAGCTT CGAAAGACTT TGATGAACAA AGTGATCGTA AGGAACCTCT TGAAGAAAAT 
GATCCTGAGA TAACTCCTGG AATAGTAATA AGTAACAATC CGGTTGAATC TTGGATTGAT 
GATGAACTAG AAGAGAAACG TCAGCAGCGG CAGAAGGAAA ATGAACAGTA CCCACCAGAA 
GCTCAACAAG CACCATCTCC AGTACCTGCG GGAATGATTC ATATTCCTGA AACATTATCC 
AAAGAGGAAA GAGCCGTTCA ACCAACTCTT GAAATGATCT TAGCCGATCC AGCAACTGTC 
GCTCCTATCA ATGCTCAAAA TATTGTTTGG AAACAGCGCG ACACTCGAGT TCCAAACCCA 
TTCCCCTCAT CGAATCAGCC ACCTGTCGCA TTTGAAGCTG CTGGATCTCC AGACACGGAT 
GCTTATTCAA CCCTTCCACA CGTTATCGTC GTCTACGTGG TTAATCCGTT TTCATACGGA 
CCAGAAGGAC ATTCAGCATT GCATATGAGA ATAGCCATTC TTGCATTCAT TCGGGCATTC 
AATTCGATTA TGTGCAAAAT TCCTTACGAA AAACGTCCTC AACTTCAGCT GGAAATCGTT 
GGAATGGAAG GCATGGATAA TGTTGCAAAG CCGATTCCAG ATTACTTCAA TGATGCCAAG 
ATTCCATTCG ATTTACTCAA TGATCGTCCG ATTCGAGTCG AACGACCTGG AGAGTCTGTT 
CAAGGAGAGC TAGCTCGGTC TTTATCAATT GCTGTATACA CCCATCCACG TGTATTCTTC 
CCGGATGTCT ACAAGTCTGC TTCAGCAAGA TGTATGACAG CATTTGGTCC TGGAAGTCAG 
CTGATGAATA CAATCAACAA AATTGAGGCG CTAAACAAGG ACTCATTTGC TCGAATGGCA 
AAACGATCAA AGACTACACT GGACACCATG GACATGTACC GACATCCAGG AATGATTCAA 
GCACAGCAGT CGACAGAAAA GAAAAACTAT ATTGCTTATC GAGTTCCATC GAACATCGCT 
GTTCTTGCAC CTCCACCAAT GGTATATCAA ATGGATGAAA AAGGAAAAGC GATAATGAAT 
CAACTGGATG AGCAAACTCT TTTCATCTCA TATTGCCTTG TTGGTACTGA TTTCCTTGTA 
GCTACAGCAA CTGATGCTCA AGGAAAACTG ATTGATAACT GTATTTCGAA TATCAAACCA 
AGACGCCAGA GCAATCAGGT GTATCGATAC AGAAATAAGA CACAAATCCT AGATGGAATG 
GGAAAACTTT GGTCATTTAT TCTTGGAATC ATGGCTTCGG AAACTAAAAA TTGGAGACTT 
GTTGTTGGAA GACTCGGACG CATCGGACAT GGAGAGTTCA GAGCCTGGAC TCATCTTCTC 
AATAAAACTT CTCTTCTTCG TTATTCGGGA AGTTTGAAGG ATATTTGTGG TGCCTGTAGA 
AGCATGCCTT CAGTCATTGG AACTCCAGCT ATTCTCTCAG CATGTCTCAT TACTCTTGAG 
CCTGAACCAA GTATTCGAAT CATGCCAGAG TTCCATGATC AGGAATTGTC AACAAAGAAG 
AGCTTTCTAT TCCAAACTCC TGGAGATTTA TCATGCACTC ACATTCTCAC TTTCCCAGTT 
GGAACCGAGA TTAACCTAGA AGTTCAAGAC CAAACTGCGG ATACCAAAGC AGATGAAAAT 
TGGGAGTTCG GAGATCTTGA CATCATGGAA GGTCTTGATG ATGGAGATAC TGAAATCATG 
AAAGATCTTG GACTGGAAAC TCCAAGCAGT GCTGCAATTC GACAGACTGG CGGACCTTCA 
ATGTTTTTCA GCGAAGATTC GTCTTCCATT GAAATTCAAA ATCAACCTCT TGCATCTGGA 
TACTACATCT CCACAGCACC GGCTCCCGAA CTTCCAGCAT GGTTCTGGGC AACATGTCCA 
TCTGCAAAAC GTCATTCTCC TGTTCATCTG AAATCCTCGT TACATATTCA TATCAGTGAA 
GTAAAGAACG ACGATATTGC AATGGAATCT ACAAAAGAAA AAGAAAAGGA CAAGGAAAAA 
GATGAAAAAG ATGCTCATCC ATTGGAATCT CGACAAACTG AGGAAGTGCT TCGACATGTA 
CTCGAGTCGT ATAATGCTCT CTCGTGGCTC AATCTCAATC GACAAACTGG TGACCGTTAC 
AGTTGTCTGC CAATTCACAT TCAACATCTT CTGAGACTCT ACCACAGTGT GGCTCGTCTA 
CTTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-19 ( NM_063465.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495866.2 Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), partial mRNA.

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CloneID	OCa337551
Clone ID Related Accession (Same CDS sequence)	NM_063465.5 , NM_063465.4
Accession Version	NM_063465.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Mediator of RNA polymerase II transcription subunit 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063465.3. COMPLETENESS: incomplete on both ends.

ATGTCCTCCG CCAAGGATAG CGACAAGGAT GCCTCACGCA AAATGAAGCT CCAGAAGCGT 
CCAGAGTGGA ACAACGGTGG CAGCTTAGAA GACTGTATCT CAAATGTTTA TGCACTACTT 
GAATTGCCAG GCATTAAATG GAAATGTTAC CGCACAAAAC CAAATGCTCC ACGTGGTGTT 
GCACTGACTG CTGATTTTGT ACTCAAAGCA TACAGTAAAT GTTTATTGGA CGGAATTCTT 
TGCACATGGA GACGTAAACC ATTACCCCCA ACTGATAACA AAGAAACTCC AAAACTGACG 
AATTTTTCAA ATGATGCTCC AAAAGAGCTC TGGGTATTTT GGTATGATGA TGAGCCAGCA 
TTGCTTCAGA AAAATTGTGA AGGATTGGAT AACGACGATG AATTATCATC AGCAAATCAA 
ATGAATATTG TGAGCTACGA AGTACGCACA ATTATCTTCA AAGCTTTGCA CGTTGTTTTG 
GAAAGAGATC TTACAAAAGA CGGATTCGTT CGATTTGGAA GATGGTTTAC AGTTCCATAT 
GATGCACGAG AAAATTATCT ACATTTTACG TATCCATCAC ATTCTCCAGC AATTCGATTC 
AACTTTTTTG TTCATGGATC CAGCACGATA TGTGCTTCTG TTCAAGCACA AAGGCAACCA 
ACTTTGATAA CTTTGGCTAG ACGACATTTG GAGTGCAAAA CAAAACGTGT TGTGGTTGTA 
GTAGGTCCAT GGTCCATGCG CGGTCAACTT GTTCAAGACC AAATAGCTCT TCTTGCCGAC 
CCAAAAATTC AAGAATCAGC AGAAAAAGAA TGGAATCAAT GGAAAGATTA TTTGCAAATG 
GAAGAAAAAG AACCAGAAAA TGTGTCAGAA GAAAGGCAAA AATCTCCAGA TTCGGATCCA 
CCAGCTCCAC CACAAACAAC TCGAGTGTTA CTTGCAGATT CATCTGATGA AGAGCAATCA 
CAGAGTTTTG AGGCAATTGA TGAGTCAAAA AAAGACGATA TTCCACAGAG ATTACGATGG 
ATGTACGAGC CTGATTACCG TCAAGAAAAA TCAAAAGAAG AGACACCAGA AGAAAAAGAA 
AAAAGAGAAC AATCAAGAGA AGTGAGGAAA CAAAGAAGGA AATTAAGAGA AGAACGACGA 
AAAGAGGTTG AAAGGCAACG TACAGAAGTT AAAAATCCTG ATGATTATGA TTCGGATGTA 
GTGACAGATG AAGATGTTGG AGAGAAGGAA GAATGTGTGA ATAATGATGT GCCGAAAATG 
GTTCTTCTTG ATATTGACGG TGTTCGATTA TTATACCCAT CGAAATTCCT TTGTGTCACA 
ATTGATGAGG ATCGGCAAAT GCTTGAGTCA ATTGGTCTGA AGACAAATCA ACAACCACCT 
GAATTACAAG CCCAAAATCG TCGCCCACGT AACAAACTTG TTGTCAACGT TGCGAATCCA 
ATTCTATCTA CAATGGCAGT CTACCAATAC TGTGAAAATG AACGGTTAAT TTCAAAAGAA 
AATGTTCCAC AAAAAAGTGC CACTAGTGGG CAGCTCTTGC CCCCACTAAA AGAAAAATTC 
TTAAATTGGA AGTTTACTGC AAAAAAGTAT GGATGCAGAG GAGATGTATC ATGTCGACAT 
TGTGCTGAAG CTGAATACCA AGGAGAATCC GATGGACAAG TTATACTAGA CGTTGATCGT 
CACAATAGAT TTCTAAAATA TGGTCCATAT ACGGAAAATT CGATTAGATG GCACAAAATT 
CGGAGTCGAA TTCAAAAGAA ATTTGATTTT CATGCCTATC ACAAGAAGCA TCCAATTGGG 
CCAATTACAC CTAGAAAGGC TGGAGACAGT GTGAAACAGA CAAAACAACT GAGTGCGGTC 
AAACGAAGAA GGAGAGCTCG TCGAGAAAGA GCACAAAAGA AGAGGCGTGA CAAAAAACGT 
CCAACTGGAT CAACTCGAGC TGATAGCGAA GATGATTCAT TTCGAATGGA CAAACTTTCT 
CAGGATGATT GGGCTAATTT TGAGGGAGAG CACCCAAAGG GAATCCCTGA AATTGAAGTT 
GATAAGGATT TTGAACTTCA TAATGACATC AAAATTCCCA AAGTTCCAAA TGATGACAAC 
TACAAGCCAA AATCGTTTTT GCCAATTACA TATCTCACCA ACAATGTAGA TAATAATGAC 
TTTGAGTCAA TGCCTGGAGA TGATGATATG TCTGAAGAGC GTTTCAATCA GATATTCAAA 
GACAAATACA ATCGATGGAA GCCTGGAATG TCGAGAGCAG AAAAAATCGA GCACGCTCAT 
TATCAATTTG ATCCATCAAA TCAAATGACC GGTTCACCAC GAGAATACCA TTTGAAGGAT 
TTGCCAAAGA AGATTCAAAA ATATGAATCA ATACTTCCTA CACCAAAAGG AAATAAGTTT 
TTCAAAGGAA GCATTTGTCG AAAAGGATTA AGAGGTTATG ATGAAATTTG TGCGGATCAA 
GATGAAAAAG AAATGATGAG AAAAGCAAAA TGGTTGAAGA AAGCAAGAAG GAAAGTTAGC 
AAACAACAGA TAAAGAAGGC GGGGCAGTAT CATATTGATA TGCAGAAACA AATAGTCAAA 
CGTTCATTGA AAAGAGCCAT CAGGAAATTA AAACTAACTA CCAAAAAGGA TTCACAATCG 
AAAGGAACAA AACAGCAGAA ACTCGTTCTA TTATCATATA AAAAGAAGAA AGTCGACGAG 
AAAAAGTTCC AACAATACCT GAAAGAAGTG GAAAATGTTT TAGACAAACT TGATGTAAAC 
TCATTTTGTA AGAAAAATGA GAAAGCTGGA TTTTCGGAGA AAGCAATACC AAGTGGATAT 
ATTATCCCTG ACTATGAATC GTCAATTCAC TGCGGGATGG AAGAAGAATC TGATTCTGAG 
ATAGACGAGC CACGCCATGG ACTTGGAGAA GGAACTTGTG GGCAATATCT GGAGGGAGAA 
TCTGTGGATC TTGCTGACAT GGAACATCAT CATAACGAAT TGCCTATGGA AGTGACCAGT 
ATCGGACATA TCAGAACAGT CGGCGAGCAT ATTGGTCCAG ATGGAATGTT ATCGCCACCT 
GCCAGCAACG AAATGCCAAA AGGAGGACCT CTATCGGTGG GTCCACCATC CATCGAGTCG 
CAAGGACTCA ATCAGATCTA CCCGACGCCG CCATCAGTTC AGATGCTCCT GGGAGATGCA 
GCTCAAGCAC ATTCTCCTGT TATGCATACA AAGTCACGTT ACGCGATGAA TACGTTGGAA 
GATGATGTTG AAAGAATCGC TGTGCCTGGT GGAGTCGGAA GTCTTGTGGA AATCGAGGCT 
GACGATATTG AAACAAAGAA TATGCAAAAG TGGAGCAAAC TTTCAAATCA ATCAAAACTG 
TCGAAGTTCA TTGCATTGTC TTGCAAAGAT CCGTTCCTGA AATCGAAGAA GAAGAAATAT 
GATACACCTG TCACTAGCAA GTTTAAGATT GAAAATGAAC CTCCAAGTAT GAGCGCAGAG 
CTGGTGTACG CACCGATGGT CACTCGCAAC TTCTACTCGG CGCCGCATCG AACATTTGGT 
ACCTTGGCTC AGAAAGTGGT TGACAAACGA CCTTCTCAAT TTGCAATCCC TTCAACATCA 
ACAGCAGCTC ATCCTGGATT GACTACACCT ATGGCATCAA TTTTCCAATC ACCCACAATG 
CCACCTCCAC ATACCCCATC ACCTTTTTCT GCTGGTCCAA TGAGTCATCA TCCAATGGGA 
CCACCTGCGT ATCCAGGAAC TCCTGGCTCA TTTCCACCAA CAACGCCAAC ATATCCTGGA 
ATGACACCAC GACCAGGGCC AAACTTTGGA CCTCCTGGTC CAGTCTATCC AAACTCTCAA 
CAAATGATGG GCCCGGGTGG ATATCCACCA CAAATGGGTA TGATAAACTC TCAACGGCAT 
TTCCAAAATC AGCAGATGTA TCAACAGCAA ATGGTGAGAA TGCAGCAAAT GCGCCAGATG 
GGTGGACCTC CAGGGTATGG TGCACCTGGT GTATCTCCTT ATCAACAGGT TAACGCAATT 
ACAAGTCCAG CAGGATTTGG AATGCCACCC AACGGTCCGT CTTCTGTTCA AAGGCTTAAC 
AATTTTGTTA ATCAATCACA ATTCGGCCCG AACAATGGTC ATCCAACACC ACCGTACGGT 
TATATGCAAC AAATGATTCC ACAATCACCA AACTTCAATC AACAGTACAA TGGAATGGGA 
AATCAACTAA TGAGTCCAAT ACATCAACAC CAATTCCATC AGCAACAAAT GGTTCAGCAG 
CAGCAGCAAC AACAAATGCA GATGCATAGA CAACAGATGC AGATGCAAAA TCAAAATATG 
TCCCAACCGT TCCAACCACC AACTGCTCAA ATGTTACAAA TGCAGCAAAA TGAGCAAATG 
AGAATAGCCT TTTCGACATT GTCAGAACGT GATAAGGCTA AATTCCAAAA ACGACAAGGT 
CAACTGAGGA GTGGAATTAT GCCCAAATCA CTACAGCCAG CAGTTCTAAA GAATCTGAAA 
AATCCACCTG ATGCTAACAT TCCATATATG AAACAGGATG CATTATTTCG ACCCCCAGAA 
TCGAATTCAC TTCAAGGAGA GAGTCTTACA ATTGCAATTG TTCTCTCGGA TACACTTCTT 
GATTTACATT TTGATTCTGT GTTTGATGCT TGCCCAATCT GTTCTTGTTC AGTTTCAATT 
CGTTCTCGAG ATATTGGAAT GTACATCACT CCACATACAG TCCTCTACTC CACAGAACGT 
GGACATACAA ATATGAGAGA ACACACTACT GGACCATGGA GTGGTTTCAA TGTCAATTCG 
GCAACTAATT GTACTTGTGG ATTTAGTGCA ATTCGACACC GCTATCTGTC TTGCTGTTCT 
GGCCTTTTTG AAGAAGATGC TGATGAAGCA ACAGCTTCAG AGCATGCAGT GGCACCAGTC 
ATTCCTCCTT TGGATTATCC GAAAAAATCA ACTGCACGGG ATATGTCTTG GTTCGATTCA 
AAATCAGTTC ACGACCTGGC TCTTCTTGAT CAAATCCGAC AAATGGCTTT CTCGAACAGT 
CTTGGAAAAG CGGTTTCACA TATGGCAACG ATCAAAGAAC ACAATAGAAA TCTTGCGATT 
GCTGTTGATC TTGGCAATGA TGTTACTGTT CCCTCCGAAT ATATCCTTTC TCATGTCGAT 
GCTTTGGAAC TTCTGATGTT GGGAAATTCG GTTCTGGGTC CAATGAAAAA CACAAGTATT 
GCTAATCAAG TTGCATCTCA CAATAAGTTC CTTGGATTTT TCCATCCATG GGGAATTCAA 
ATTGCAAATG GAATGACTGA ACTTGAAGCA TCGGAATGGG TTGACTTGCT TGGCGTTGTA 
ACCCCAACAT TGGAAGGTTC AATGAAACAA GCAAGACACA TACCTGCAGA AACTCCATTT 
GAACTTGATG GTCCAATGAC GTGGAAACAA ATTGTCACCA AGAGTAACAG AGGAAAACCT 
CCAGCAGATG ATGAAGAAGA TTTTTCTCTA CCTGAACCAG TTCCTGCAAT AATGAGAGCA 
TTGACAAAGG AAGCAATTCG AGCTGCTCCA AACATTGAAC AATACTACGA ACAAGCAGGA 
TTAGGACCAG TGGATCAGCC AAAAGATGTC ATGTACATTA CAGTTATTCC AGACGATGAT 
GACATTTATT CACGAACTGT CGAATTCATG GATACTTTGA CACGTACTTA TGAAAAAATG 
AGACTAGGAC GTCATATTCC ATTCCCTGTC TCAACTGGTA CAGCAACTCG TTTCCGCGAA 
GCATTCAAGT CATGTCAATC TCAATTCCCA TTCCAAGAGC AACAACACTT GGCATACACA 
AATCAAACAC TTCGAAATAT GCCTCAAACA TTGGCTGATA CTCCAGACCC GGTGAACAGC 
GATCCAAAAG TTTGGTCGAG GCGAGCGAAG AGAAAGTTGG ATTTTGAAGA AGAACTGAAA 
CTCCCTGAAG CTGGCTCAGA AACTATTGAT GAATCCGATG ATGACGAAGA AAACGAAGAA 
GTTTCAAAAG AAGAGAACAA CGAGCTCATT GATTCAGAAG ACGTTATGGA CGAGGTTCCA 
AAAGGTTTCT ACGAACGGGA AGGAATTTTG CGTGTTGGGG CTCCAATGGA GCCGTCAAGA 
AGTCCGCATA CTGTAACAAA CACTCCTGAA TTTGACAACA TGACAAAATA TCTGAGTGAT 
GAGAATGGAT TTGTGAGCAG ACTTCGGCTT TATTTACAAC AAATGGAGGA TCTAGTTCAT 
TTTGCATTGA GTGAGAATCC AGAAGCGTTT GATAGAAGAG GGTATCGATA TCAATTAGCA 
GTTGAAGGAA GACTCAAGAG ACAGAAACAT AAAATGGATA TCATAGAAGA ACGTCTCAGA 
TTTGAAGCTT CGAAAGACTT TGATGAACAA AGTGATCGTA AGGAACCTCT TGAAGAAAAT 
GATCCTGAGA TAACTCCTGG AATAGTAATA AGTAACAATC CGGTTGAATC TTGGATTGAT 
GATGAACTAG AAGAGAAACG TCAGCAGCGG CAGAAGGAAA ATGAACAGTA CCCACCAGAA 
GCTCAACAAG CACCATCTCC AGTACCTGCG GGAATGATTC ATATTCCTGA AACATTATCC 
AAAGAGGAAA GAGCCGTTCA ACCAACTCTT GAAATGATCT TAGCCGATCC AGCAACTGTC 
GCTCCTATCA ATGCTCAAAA TATTGTTTGG AAACAGCGCG ACACTCGAGT TCCAAACCCA 
TTCCCCTCAT CGAATCAGCC ACCTGTCGCA TTTGAAGCTG CTGGATCTCC AGACACGGAT 
GCTTATTCAA CCCTTCCACA CGTTATCGTC GTCTACGTGG TTAATCCGTT TTCATACGGA 
CCAGAAGGAC ATTCAGCATT GCATATGAGA ATAGCCATTC TTGCATTCAT TCGGGCATTC 
AATTCGATTA TGTGCAAAAT TCCTTACGAA AAACGTCCTC AACTTCAGCT GGAAATCGTT 
GGAATGGAAG GCATGGATAA TGTTGCAAAG CCGATTCCAG ATTACTTCAA TGATGCCAAG 
ATTCCATTCG ATTTACTCAA TGATCGTCCG ATTCGAGTCG AACGACCTGG AGAGTCTGTT 
CAAGGAGAGC TAGCTCGGTC TTTATCAATT GCTGTATACA CCCATCCACG TGTATTCTTC 
CCGGATGTCT ACAAGTCTGC TTCAGCAAGA TGTATGACAG CATTTGGTCC TGGAAGTCAG 
CTGATGAATA CAATCAACAA AATTGAGGCG CTAAACAAGG ACTCATTTGC TCGAATGGCA 
AAACGATCAA AGACTACACT GGACACCATG GACATGTACC GACATCCAGG AATGATTCAA 
GCACAGCAGT CGACAGAAAA GAAAAACTAT ATTGCTTATC GAGTTCCATC GAACATCGCT 
GTTCTTGCAC CTCCACCAAT GGTATATCAA ATGGATGAAA AAGGAAAAGC GATAATGAAT 
CAACTGGATG AGCAAACTCT TTTCATCTCA TATTGCCTTG TTGGTACTGA TTTCCTTGTA 
GCTACAGCAA CTGATGCTCA AGGAAAACTG ATTGATAACT GTATTTCGAA TATCAAACCA 
AGACGCCAGA GCAATCAGGT GTATCGATAC AGAAATAAGA CACAAATCCT AGATGGAATG 
GGAAAACTTT GGTCATTTAT TCTTGGAATC ATGGCTTCGG AAACTAAAAA TTGGAGACTT 
GTTGTTGGAA GACTCGGACG CATCGGACAT GGAGAGTTCA GAGCCTGGAC TCATCTTCTC 
AATAAAACTT CTCTTCTTCG TTATTCGGGA AGTTTGAAGG ATATTTGTGG TGCCTGTAGA 
AGCATGCCTT CAGTCATTGG AACTCCAGCT ATTCTCTCAG CATGTCTCAT TACTCTTGAG 
CCTGAACCAA GTATTCGAAT CATGCCAGAG TTCCATGATC AGGAATTGTC AACAAAGAAG 
AGCTTTCTAT TCCAAACTCC TGGAGATTTA TCATGCACTC ACATTCTCAC TTTCCCAGTT 
GGAACCGAGA TTAACCTAGA AGTTCAAGAC CAAACTGCGG ATACCAAAGC AGATGAAAAT 
TGGGAGTTCG GAGATCTTGA CATCATGGAA GGTCTTGATG ATGGAGATAC TGAAATCATG 
AAAGATCTTG GACTGGAAAC TCCAAGCAGT GCTGCAATTC GACAGACTGG CGGACCTTCA 
ATGTTTTTCA GCGAAGATTC GTCTTCCATT GAAATTCAAA ATCAACCTCT TGCATCTGGA 
TACTACATCT CCACAGCACC GGCTCCCGAA CTTCCAGCAT GGTTCTGGGC AACATGTCCA 
TCTGCAAAAC GTCATTCTCC TGTTCATCTG AAATCCTCGT TACATATTCA TATCAGTGAA 
GTAAAGAACG ACGATATTGC AATGGAATCT ACAAAAGAAA AAGAAAAGGA CAAGGAAAAA 
GATGAAAAAG ATGCTCATCC ATTGGAATCT CGACAAACTG AGGAAGTGCT TCGACATGTA 
CTCGAGTCGT ATAATGCTCT CTCGTGGCTC AATCTCAATC GACAAACTGG TGACCGTTAC 
AGTTGTCTGC CAATTCACAT TCAACATCTT CTGAGACTCT ACCACAGTGT GGCTCGTCTA 
CTTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495866.2
CDS	1..8589
Translation	MSSAKDSDKDASRKMKLQKRPEWNNGGSLEDCISNVYALLELPGIKWKCYRTKPNAPRGVALTADFVLKAYSKCLLDGILCTWRRKPLPPTDNKETPKLTNFSNDAPKELWVFWYDDEPALLQKNCEGLDNDDELSSANQMNIVSYEVRTIIFKALHVVLERDLTKDGFVRFGRWFTVPYDARENYLHFTYPSHSPAIRFNFFVHGSSTICASVQAQRQPTLITLARRHLECKTKRVVVVVGPWSMRGQLVQDQIALLADPKIQESAEKEWNQWKDYLQMEEKEPENVSEERQKSPDSDPPAPPQTTRVLLADSSDEEQSQSFEAIDESKKDDIPQRLRWMYEPDYRQEKSKEETPEEKEKREQSREVRKQRRKLREERRKEVERQRTEVKNPDDYDSDVVTDEDVGEKEECVNNDVPKMVLLDIDGVRLLYPSKFLCVTIDEDRQMLESIGLKTNQQPPELQAQNRRPRNKLVVNVANPILSTMAVYQYCENERLISKENVPQKSATSGQLLPPLKEKFLNWKFTAKKYGCRGDVSCRHCAEAEYQGESDGQVILDVDRHNRFLKYGPYTENSIRWHKIRSRIQKKFDFHAYHKKHPIGPITPRKAGDSVKQTKQLSAVKRRRRARRERAQKKRRDKKRPTGSTRADSEDDSFRMDKLSQDDWANFEGEHPKGIPEIEVDKDFELHNDIKIPKVPNDDNYKPKSFLPITYLTNNVDNNDFESMPGDDDMSEERFNQIFKDKYNRWKPGMSRAEKIEHAHYQFDPSNQMTGSPREYHLKDLPKKIQKYESILPTPKGNKFFKGSICRKGLRGYDEICADQDEKEMMRKAKWLKKARRKVSKQQIKKAGQYHIDMQKQIVKRSLKRAIRKLKLTTKKDSQSKGTKQQKLVLLSYKKKKVDEKKFQQYLKEVENVLDKLDVNSFCKKNEKAGFSEKAIPSGYIIPDYESSIHCGMEEESDSEIDEPRHGLGEGTCGQYLEGESVDLADMEHHHNELPMEVTSIGHIRTVGEHIGPDGMLSPPASNEMPKGGPLSVGPPSIESQGLNQIYPTPPSVQMLLGDAAQAHSPVMHTKSRYAMNTLEDDVERIAVPGGVGSLVEIEADDIETKNMQKWSKLSNQSKLSKFIALSCKDPFLKSKKKKYDTPVTSKFKIENEPPSMSAELVYAPMVTRNFYSAPHRTFGTLAQKVVDKRPSQFAIPSTSTAAHPGLTTPMASIFQSPTMPPPHTPSPFSAGPMSHHPMGPPAYPGTPGSFPPTTPTYPGMTPRPGPNFGPPGPVYPNSQQMMGPGGYPPQMGMINSQRHFQNQQMYQQQMVRMQQMRQMGGPPGYGAPGVSPYQQVNAITSPAGFGMPPNGPSSVQRLNNFVNQSQFGPNNGHPTPPYGYMQQMIPQSPNFNQQYNGMGNQLMSPIHQHQFHQQQMVQQQQQQQMQMHRQQMQMQNQNMSQPFQPPTAQMLQMQQNEQMRIAFSTLSERDKAKFQKRQGQLRSGIMPKSLQPAVLKNLKNPPDANIPYMKQDALFRPPESNSLQGESLTIAIVLSDTLLDLHFDSVFDACPICSCSVSIRSRDIGMYITPHTVLYSTERGHTNMREHTTGPWSGFNVNSATNCTCGFSAIRHRYLSCCSGLFEEDADEATASEHAVAPVIPPLDYPKKSTARDMSWFDSKSVHDLALLDQIRQMAFSNSLGKAVSHMATIKEHNRNLAIAVDLGNDVTVPSEYILSHVDALELLMLGNSVLGPMKNTSIANQVASHNKFLGFFHPWGIQIANGMTELEASEWVDLLGVVTPTLEGSMKQARHIPAETPFELDGPMTWKQIVTKSNRGKPPADDEEDFSLPEPVPAIMRALTKEAIRAAPNIEQYYEQAGLGPVDQPKDVMYITVIPDDDDIYSRTVEFMDTLTRTYEKMRLGRHIPFPVSTGTATRFREAFKSCQSQFPFQEQQHLAYTNQTLRNMPQTLADTPDPVNSDPKVWSRRAKRKLDFEEELKLPEAGSETIDESDDDEENEEVSKEENNELIDSEDVMDEVPKGFYEREGILRVGAPMEPSRSPHTVTNTPEFDNMTKYLSDENGFVSRLRLYLQQMEDLVHFALSENPEAFDRRGYRYQLAVEGRLKRQKHKMDIIEERLRFEASKDFDEQSDRKEPLEENDPEITPGIVISNNPVESWIDDELEEKRQQRQKENEQYPPEAQQAPSPVPAGMIHIPETLSKEERAVQPTLEMILADPATVAPINAQNIVWKQRDTRVPNPFPSSNQPPVAFEAAGSPDTDAYSTLPHVIVVYVVNPFSYGPEGHSALHMRIAILAFIRAFNSIMCKIPYEKRPQLQLEIVGMEGMDNVAKPIPDYFNDAKIPFDLLNDRPIRVERPGESVQGELARSLSIAVYTHPRVFFPDVYKSASARCMTAFGPGSQLMNTINKIEALNKDSFARMAKRSKTTLDTMDMYRHPGMIQAQQSTEKKNYIAYRVPSNIAVLAPPPMVYQMDEKGKAIMNQLDEQTLFISYCLVGTDFLVATATDAQGKLIDNCISNIKPRRQSNQVYRYRNKTQILDGMGKLWSFILGIMASETKNWRLVVGRLGRIGHGEFRAWTHLLNKTSLLRYSGSLKDICGACRSMPSVIGTPAILSACLITLEPEPSIRIMPEFHDQELSTKKSFLFQTPGDLSCTHILTFPVGTEINLEVQDQTADTKADENWEFGDLDIMEGLDDGDTEIMKDLGLETPSSAAIRQTGGPSMFFSEDSSSIEIQNQPLASGYYISTAPAPELPAWFWATCPSAKRHSPVHLKSSLHIHISEVKNDDIAMESTKEKEKDKEKDEKDAHPLESRQTEEVLRHVLESYNALSWLNLNRQTGDRYSCLPIHIQHLLRLYHSVARLLA

Target ORF information:

RefSeq Version	NM_063465.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Mediator of RNA polymerase II transcription subunit 13 (let-19), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063465.4

ORF Insert Sequence:

ATGTCCTCCG CCAAGGATAG CGACAAGGAT GCCTCACGCA AAATGAAGCT CCAGAAGCGT 
CCAGAGTGGA ACAACGGTGG CAGCTTAGAA GACTGTATCT CAAATGTTTA TGCACTACTT 
GAATTGCCAG GCATTAAATG GAAATGTTAC CGCACAAAAC CAAATGCTCC ACGTGGTGTT 
GCACTGACTG CTGATTTTGT ACTCAAAGCA TACAGTAAAT GTTTATTGGA CGGAATTCTT 
TGCACATGGA GACGTAAACC ATTACCCCCA ACTGATAACA AAGAAACTCC AAAACTGACG 
AATTTTTCAA ATGATGCTCC AAAAGAGCTC TGGGTATTTT GGTATGATGA TGAGCCAGCA 
TTGCTTCAGA AAAATTGTGA AGGATTGGAT AACGACGATG AATTATCATC AGCAAATCAA 
ATGAATATTG TGAGCTACGA AGTACGCACA ATTATCTTCA AAGCTTTGCA CGTTGTTTTG 
GAAAGAGATC TTACAAAAGA CGGATTCGTT CGATTTGGAA GATGGTTTAC AGTTCCATAT 
GATGCACGAG AAAATTATCT ACATTTTACG TATCCATCAC ATTCTCCAGC AATTCGATTC 
AACTTTTTTG TTCATGGATC CAGCACGATA TGTGCTTCTG TTCAAGCACA AAGGCAACCA 
ACTTTGATAA CTTTGGCTAG ACGACATTTG GAGTGCAAAA CAAAACGTGT TGTGGTTGTA 
GTAGGTCCAT GGTCCATGCG CGGTCAACTT GTTCAAGACC AAATAGCTCT TCTTGCCGAC 
CCAAAAATTC AAGAATCAGC AGAAAAAGAA TGGAATCAAT GGAAAGATTA TTTGCAAATG 
GAAGAAAAAG AACCAGAAAA TGTGTCAGAA GAAAGGCAAA AATCTCCAGA TTCGGATCCA 
CCAGCTCCAC CACAAACAAC TCGAGTGTTA CTTGCAGATT CATCTGATGA AGAGCAATCA 
CAGAGTTTTG AGGCAATTGA TGAGTCAAAA AAAGACGATA TTCCACAGAG ATTACGATGG 
ATGTACGAGC CTGATTACCG TCAAGAAAAA TCAAAAGAAG AGACACCAGA AGAAAAAGAA 
AAAAGAGAAC AATCAAGAGA AGTGAGGAAA CAAAGAAGGA AATTAAGAGA AGAACGACGA 
AAAGAGGTTG AAAGGCAACG TACAGAAGTT AAAAATCCTG ATGATTATGA TTCGGATGTA 
GTGACAGATG AAGATGTTGG AGAGAAGGAA GAATGTGTGA ATAATGATGT GCCGAAAATG 
GTTCTTCTTG ATATTGACGG TGTTCGATTA TTATACCCAT CGAAATTCCT TTGTGTCACA 
ATTGATGAGG ATCGGCAAAT GCTTGAGTCA ATTGGTCTGA AGACAAATCA ACAACCACCT 
GAATTACAAG CCCAAAATCG TCGCCCACGT AACAAACTTG TTGTCAACGT TGCGAATCCA 
ATTCTATCTA CAATGGCAGT CTACCAATAC TGTGAAAATG AACGGTTAAT TTCAAAAGAA 
AATGTTCCAC AAAAAAGTGC CACTAGTGGG CAGCTCTTGC CCCCACTAAA AGAAAAATTC 
TTAAATTGGA AGTTTACTGC AAAAAAGTAT GGATGCAGAG GAGATGTATC ATGTCGACAT 
TGTGCTGAAG CTGAATACCA AGGAGAATCC GATGGACAAG TTATACTAGA CGTTGATCGT 
CACAATAGAT TTCTAAAATA TGGTCCATAT ACGGAAAATT CGATTAGATG GCACAAAATT 
CGGAGTCGAA TTCAAAAGAA ATTTGATTTT CATGCCTATC ACAAGAAGCA TCCAATTGGG 
CCAATTACAC CTAGAAAGGC TGGAGACAGT GTGAAACAGA CAAAACAACT GAGTGCGGTC 
AAACGAAGAA GGAGAGCTCG TCGAGAAAGA GCACAAAAGA AGAGGCGTGA CAAAAAACGT 
CCAACTGGAT CAACTCGAGC TGATAGCGAA GATGATTCAT TTCGAATGGA CAAACTTTCT 
CAGGATGATT GGGCTAATTT TGAGGGAGAG CACCCAAAGG GAATCCCTGA AATTGAAGTT 
GATAAGGATT TTGAACTTCA TAATGACATC AAAATTCCCA AAGTTCCAAA TGATGACAAC 
TACAAGCCAA AATCGTTTTT GCCAATTACA TATCTCACCA ACAATGTAGA TAATAATGAC 
TTTGAGTCAA TGCCTGGAGA TGATGATATG TCTGAAGAGC GTTTCAATCA GATATTCAAA 
GACAAATACA ATCGATGGAA GCCTGGAATG TCGAGAGCAG AAAAAATCGA GCACGCTCAT 
TATCAATTTG ATCCATCAAA TCAAATGACC GGTTCACCAC GAGAATACCA TTTGAAGGAT 
TTGCCAAAGA AGATTCAAAA ATATGAATCA ATACTTCCTA CACCAAAAGG AAATAAGTTT 
TTCAAAGGAA GCATTTGTCG AAAAGGATTA AGAGGTTATG ATGAAATTTG TGCGGATCAA 
GATGAAAAAG AAATGATGAG AAAAGCAAAA TGGTTGAAGA AAGCAAGAAG GAAAGTTAGC 
AAACAACAGA TAAAGAAGGC GGGGCAGTAT CATATTGATA TGCAGAAACA AATAGTCAAA 
CGTTCATTGA AAAGAGCCAT CAGGAAATTA AAACTAACTA CCAAAAAGGA TTCACAATCG 
AAAGGAACAA AACAGCAGAA ACTCGTTCTA TTATCATATA AAAAGAAGAA AGTCGACGAG 
AAAAAGTTCC AACAATACCT GAAAGAAGTG GAAAATGTTT TAGACAAACT TGATGTAAAC 
TCATTTTGTA AGAAAAATGA GAAAGCTGGA TTTTCGGAGA AAGCAATACC AAGTGGATAT 
ATTATCCCTG ACTATGAATC GTCAATTCAC TGCGGGATGG AAGAAGAATC TGATTCTGAG 
ATAGACGAGC CACGCCATGG ACTTGGAGAA GGAACTTGTG GGCAATATCT GGAGGGAGAA 
TCTGTGGATC TTGCTGACAT GGAACATCAT CATAACGAAT TGCCTATGGA AGTGACCAGT 
ATCGGACATA TCAGAACAGT CGGCGAGCAT ATTGGTCCAG ATGGAATGTT ATCGCCACCT 
GCCAGCAACG AAATGCCAAA AGGAGGACCT CTATCGGTGG GTCCACCATC CATCGAGTCG 
CAAGGACTCA ATCAGATCTA CCCGACGCCG CCATCAGTTC AGATGCTCCT GGGAGATGCA 
GCTCAAGCAC ATTCTCCTGT TATGCATACA AAGTCACGTT ACGCGATGAA TACGTTGGAA 
GATGATGTTG AAAGAATCGC TGTGCCTGGT GGAGTCGGAA GTCTTGTGGA AATCGAGGCT 
GACGATATTG AAACAAAGAA TATGCAAAAG TGGAGCAAAC TTTCAAATCA ATCAAAACTG 
TCGAAGTTCA TTGCATTGTC TTGCAAAGAT CCGTTCCTGA AATCGAAGAA GAAGAAATAT 
GATACACCTG TCACTAGCAA GTTTAAGATT GAAAATGAAC CTCCAAGTAT GAGCGCAGAG 
CTGGTGTACG CACCGATGGT CACTCGCAAC TTCTACTCGG CGCCGCATCG AACATTTGGT 
ACCTTGGCTC AGAAAGTGGT TGACAAACGA CCTTCTCAAT TTGCAATCCC TTCAACATCA 
ACAGCAGCTC ATCCTGGATT GACTACACCT ATGGCATCAA TTTTCCAATC ACCCACAATG 
CCACCTCCAC ATACCCCATC ACCTTTTTCT GCTGGTCCAA TGAGTCATCA TCCAATGGGA 
CCACCTGCGT ATCCAGGAAC TCCTGGCTCA TTTCCACCAA CAACGCCAAC ATATCCTGGA 
ATGACACCAC GACCAGGGCC AAACTTTGGA CCTCCTGGTC CAGTCTATCC AAACTCTCAA 
CAAATGATGG GCCCGGGTGG ATATCCACCA CAAATGGGTA TGATAAACTC TCAACGGCAT 
TTCCAAAATC AGCAGATGTA TCAACAGCAA ATGGTGAGAA TGCAGCAAAT GCGCCAGATG 
GGTGGACCTC CAGGGTATGG TGCACCTGGT GTATCTCCTT ATCAACAGGT TAACGCAATT 
ACAAGTCCAG CAGGATTTGG AATGCCACCC AACGGTCCGT CTTCTGTTCA AAGGCTTAAC 
AATTTTGTTA ATCAATCACA ATTCGGCCCG AACAATGGTC ATCCAACACC ACCGTACGGT 
TATATGCAAC AAATGATTCC ACAATCACCA AACTTCAATC AACAGTACAA TGGAATGGGA 
AATCAACTAA TGAGTCCAAT ACATCAACAC CAATTCCATC AGCAACAAAT GGTTCAGCAG 
CAGCAGCAAC AACAAATGCA GATGCATAGA CAACAGATGC AGATGCAAAA TCAAAATATG 
TCCCAACCGT TCCAACCACC AACTGCTCAA ATGTTACAAA TGCAGCAAAA TGAGCAAATG 
AGAATAGCCT TTTCGACATT GTCAGAACGT GATAAGGCTA AATTCCAAAA ACGACAAGGT 
CAACTGAGGA GTGGAATTAT GCCCAAATCA CTACAGCCAG CAGTTCTAAA GAATCTGAAA 
AATCCACCTG ATGCTAACAT TCCATATATG AAACAGGATG CATTATTTCG ACCCCCAGAA 
TCGAATTCAC TTCAAGGAGA GAGTCTTACA ATTGCAATTG TTCTCTCGGA TACACTTCTT 
GATTTACATT TTGATTCTGT GTTTGATGCT TGCCCAATCT GTTCTTGTTC AGTTTCAATT 
CGTTCTCGAG ATATTGGAAT GTACATCACT CCACATACAG TCCTCTACTC CACAGAACGT 
GGACATACAA ATATGAGAGA ACACACTACT GGACCATGGA GTGGTTTCAA TGTCAATTCG 
GCAACTAATT GTACTTGTGG ATTTAGTGCA ATTCGACACC GCTATCTGTC TTGCTGTTCT 
GGCCTTTTTG AAGAAGATGC TGATGAAGCA ACAGCTTCAG AGCATGCAGT GGCACCAGTC 
ATTCCTCCTT TGGATTATCC GAAAAAATCA ACTGCACGGG ATATGTCTTG GTTCGATTCA 
AAATCAGTTC ACGACCTGGC TCTTCTTGAT CAAATCCGAC AAATGGCTTT CTCGAACAGT 
CTTGGAAAAG CGGTTTCACA TATGGCAACG ATCAAAGAAC ACAATAGAAA TCTTGCGATT 
GCTGTTGATC TTGGCAATGA TGTTACTGTT CCCTCCGAAT ATATCCTTTC TCATGTCGAT 
GCTTTGGAAC TTCTGATGTT GGGAAATTCG GTTCTGGGTC CAATGAAAAA CACAAGTATT 
GCTAATCAAG TTGCATCTCA CAATAAGTTC CTTGGATTTT TCCATCCATG GGGAATTCAA 
ATTGCAAATG GAATGACTGA ACTTGAAGCA TCGGAATGGG TTGACTTGCT TGGCGTTGTA 
ACCCCAACAT TGGAAGGTTC AATGAAACAA GCAAGACACA TACCTGCAGA AACTCCATTT 
GAACTTGATG GTCCAATGAC GTGGAAACAA ATTGTCACCA AGAGTAACAG AGGAAAACCT 
CCAGCAGATG ATGAAGAAGA TTTTTCTCTA CCTGAACCAG TTCCTGCAAT AATGAGAGCA 
TTGACAAAGG AAGCAATTCG AGCTGCTCCA AACATTGAAC AATACTACGA ACAAGCAGGA 
TTAGGACCAG TGGATCAGCC AAAAGATGTC ATGTACATTA CAGTTATTCC AGACGATGAT 
GACATTTATT CACGAACTGT CGAATTCATG GATACTTTGA CACGTACTTA TGAAAAAATG 
AGACTAGGAC GTCATATTCC ATTCCCTGTC TCAACTGGTA CAGCAACTCG TTTCCGCGAA 
GCATTCAAGT CATGTCAATC TCAATTCCCA TTCCAAGAGC AACAACACTT GGCATACACA 
AATCAAACAC TTCGAAATAT GCCTCAAACA TTGGCTGATA CTCCAGACCC GGTGAACAGC 
GATCCAAAAG TTTGGTCGAG GCGAGCGAAG AGAAAGTTGG ATTTTGAAGA AGAACTGAAA 
CTCCCTGAAG CTGGCTCAGA AACTATTGAT GAATCCGATG ATGACGAAGA AAACGAAGAA 
GTTTCAAAAG AAGAGAACAA CGAGCTCATT GATTCAGAAG ACGTTATGGA CGAGGTTCCA 
AAAGGTTTCT ACGAACGGGA AGGAATTTTG CGTGTTGGGG CTCCAATGGA GCCGTCAAGA 
AGTCCGCATA CTGTAACAAA CACTCCTGAA TTTGACAACA TGACAAAATA TCTGAGTGAT 
GAGAATGGAT TTGTGAGCAG ACTTCGGCTT TATTTACAAC AAATGGAGGA TCTAGTTCAT 
TTTGCATTGA GTGAGAATCC AGAAGCGTTT GATAGAAGAG GGTATCGATA TCAATTAGCA 
GTTGAAGGAA GACTCAAGAG ACAGAAACAT AAAATGGATA TCATAGAAGA ACGTCTCAGA 
TTTGAAGCTT CGAAAGACTT TGATGAACAA AGTGATCGTA AGGAACCTCT TGAAGAAAAT 
GATCCTGAGA TAACTCCTGG AATAGTAATA AGTAACAATC CGGTTGAATC TTGGATTGAT 
GATGAACTAG AAGAGAAACG TCAGCAGCGG CAGAAGGAAA ATGAACAGTA CCCACCAGAA 
GCTCAACAAG CACCATCTCC AGTACCTGCG GGAATGATTC ATATTCCTGA AACATTATCC 
AAAGAGGAAA GAGCCGTTCA ACCAACTCTT GAAATGATCT TAGCCGATCC AGCAACTGTC 
GCTCCTATCA ATGCTCAAAA TATTGTTTGG AAACAGCGCG ACACTCGAGT TCCAAACCCA 
TTCCCCTCAT CGAATCAGCC ACCTGTCGCA TTTGAAGCTG CTGGATCTCC AGACACGGAT 
GCTTATTCAA CCCTTCCACA CGTTATCGTC GTCTACGTGG TTAATCCGTT TTCATACGGA 
CCAGAAGGAC ATTCAGCATT GCATATGAGA ATAGCCATTC TTGCATTCAT TCGGGCATTC 
AATTCGATTA TGTGCAAAAT TCCTTACGAA AAACGTCCTC AACTTCAGCT GGAAATCGTT 
GGAATGGAAG GCATGGATAA TGTTGCAAAG CCGATTCCAG ATTACTTCAA TGATGCCAAG 
ATTCCATTCG ATTTACTCAA TGATCGTCCG ATTCGAGTCG AACGACCTGG AGAGTCTGTT 
CAAGGAGAGC TAGCTCGGTC TTTATCAATT GCTGTATACA CCCATCCACG TGTATTCTTC 
CCGGATGTCT ACAAGTCTGC TTCAGCAAGA TGTATGACAG CATTTGGTCC TGGAAGTCAG 
CTGATGAATA CAATCAACAA AATTGAGGCG CTAAACAAGG ACTCATTTGC TCGAATGGCA 
AAACGATCAA AGACTACACT GGACACCATG GACATGTACC GACATCCAGG AATGATTCAA 
GCACAGCAGT CGACAGAAAA GAAAAACTAT ATTGCTTATC GAGTTCCATC GAACATCGCT 
GTTCTTGCAC CTCCACCAAT GGTATATCAA ATGGATGAAA AAGGAAAAGC GATAATGAAT 
CAACTGGATG AGCAAACTCT TTTCATCTCA TATTGCCTTG TTGGTACTGA TTTCCTTGTA 
GCTACAGCAA CTGATGCTCA AGGAAAACTG ATTGATAACT GTATTTCGAA TATCAAACCA 
AGACGCCAGA GCAATCAGGT GTATCGATAC AGAAATAAGA CACAAATCCT AGATGGAATG 
GGAAAACTTT GGTCATTTAT TCTTGGAATC ATGGCTTCGG AAACTAAAAA TTGGAGACTT 
GTTGTTGGAA GACTCGGACG CATCGGACAT GGAGAGTTCA GAGCCTGGAC TCATCTTCTC 
AATAAAACTT CTCTTCTTCG TTATTCGGGA AGTTTGAAGG ATATTTGTGG TGCCTGTAGA 
AGCATGCCTT CAGTCATTGG AACTCCAGCT ATTCTCTCAG CATGTCTCAT TACTCTTGAG 
CCTGAACCAA GTATTCGAAT CATGCCAGAG TTCCATGATC AGGAATTGTC AACAAAGAAG 
AGCTTTCTAT TCCAAACTCC TGGAGATTTA TCATGCACTC ACATTCTCAC TTTCCCAGTT 
GGAACCGAGA TTAACCTAGA AGTTCAAGAC CAAACTGCGG ATACCAAAGC AGATGAAAAT 
TGGGAGTTCG GAGATCTTGA CATCATGGAA GGTCTTGATG ATGGAGATAC TGAAATCATG 
AAAGATCTTG GACTGGAAAC TCCAAGCAGT GCTGCAATTC GACAGACTGG CGGACCTTCA 
ATGTTTTTCA GCGAAGATTC GTCTTCCATT GAAATTCAAA ATCAACCTCT TGCATCTGGA 
TACTACATCT CCACAGCACC GGCTCCCGAA CTTCCAGCAT GGTTCTGGGC AACATGTCCA 
TCTGCAAAAC GTCATTCTCC TGTTCATCTG AAATCCTCGT TACATATTCA TATCAGTGAA 
GTAAAGAACG ACGATATTGC AATGGAATCT ACAAAAGAAA AAGAAAAGGA CAAGGAAAAA 
GATGAAAAAG ATGCTCATCC ATTGGAATCT CGACAAACTG AGGAAGTGCT TCGACATGTA 
CTCGAGTCGT ATAATGCTCT CTCGTGGCTC AATCTCAATC GACAAACTGG TGACCGTTAC 
AGTTGTCTGC CAATTCACAT TCAACATCTT CTGAGACTCT ACCACAGTGT GGCTCGTCTA 
CTTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-19 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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