lin-5 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	lin-5
Entrez Gene ID	174267
Full Name	Spindle apparatus protein lin-5
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063253.5	NP_495654.1	Spindle apparatus protein lin-5
NM_063253.6	NP_495654.1	Spindle apparatus protein lin-5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LIN-5 is required for EMS spindle positioning, possibly through a Galpha- and DLG-1-independent mechanism.
Title: Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA.

Four distinct in vivo phosphorylated LIN-5 residues were found to have critical functions in spindle positioning. Two of these residues form part of a 30 amino acid binding site for GPR-1, which we identified by reverse two-hybrid screening. These LIN-5 phosphorylations promote LIN-5-GPR-1/2 interaction and contribute to cortical pulling forces
Title: Multisite Phosphorylation of NuMA-Related LIN-5 Controls Mitotic Spindle Positioning in C. elegans.

Data show that Phospho-LIN-5 co-localizes with PKC-3 at the anterior cell cortex and temporally coincides with a switch from anterior- to posterior-directed spindle movements in the one-cell embryo.
Title: aPKC phosphorylates NuMA-related LIN-5 to position the mitotic spindle during asymmetric division.

ASPM-1, together with calmodulin (CMD-1), promotes meiotic spindle organization and the accumulation of LIN-5 at meiotic and mitotic spindle poles.
Title: NuMA-related LIN-5, ASPM-1, calmodulin and dynein promote meiotic spindle rotation independently of cortical LIN-5/GPR/Galpha.

Dynamic localization of lin-5 to cell cortex forces generation of domains during spindle positioning.
Title: Dynamic localization of LIN-5 and GPR-1/2 to cortical force generation domains during spindle positioning.

The following lin-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lin-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa339497	NM_063253.6 Latest version!	Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa339497	NM_063253.5	Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lin-5 ( NM_063253.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495654.1 Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), partial mRNA.

Price & Availability↑

CloneID	OCa339497
Clone ID Related Accession (Same CDS sequence)	NM_063253.5 , NM_063253.6
Accession Version	NM_063253.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spindle apparatus protein lin-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063253.4. COMPLETENESS: incomplete on both ends.

ATGAGCGTGA GCACATCAGT TGTGAAGTCA ATGCTACAAT TGACGAACTA TTTTGGCTCC 
AATTTCACAC CGAAGACGGA GACAATCAGC TCATTAGCCG AGCTTTGGGA GAGGCACGGG 
AGTATGATCG CCAGTATGAT GTCATGCGAC ACTCGGGAGT TTGGCACAGC TGACAAATTC 
TATGAAGTTA TCTTCCGCAA AATCGCCACA ACGAGTCTCA GCTTCATGAC CGATTCATTG 
GAACCGAAGC TTGCAGCGAG CGGAGATTCA CTGGAGATTT GCAAATTCTA CGCTCTGTTT 
CTCGAGTATA TGAGAAAGGA TCACTCGGGA ATGCTCACTC GCGCATTGGA GCAAGCGTCA 
TCAGAGAATC GCGTCGACAC AGCTGTGCTT CTTGATATGT TTTTGTTTTT CGACAACGTG 
TATTCGGAAA GTACCGCTGA CGACTGGTGG GCATGTCTTC GGAGAGATGA CGAAGATCAG 
GAAGCATCCA GTGGCTTCAG GACTCCGAAA AGGAACAACT ACAGCTTGAC ATCATTGCAA 
ACACCAACAG CTACTGCCCG GCGACTACGC ACAGCTTCCT CAACTGCTCG GCGAAGCCCT 
ATAGCGGAGG CTGTCGATTC CCCTACCATG AAGTTCATGC GAAGCGAGCG GGAGCTGAAG 
CAGTCTAAAG CGAGAATCTT TGGTTTGGAG CAAATGTTAG GAGACCTTGA AGATGACAAG 
ACCAAGTTAT CGGCTGAGAA CAGAACTTTG AAGCTTTCGA ATTCCGATCT GAAAAGCGAC 
ATCGCTAAAC TGAAAGGTAT CGCCGAGGAG GCACAATTTC GTGCTGAAGA ATTGAGCTGT 
GATCTCGAAG CCAAAAAGGA TGAAGTCCAC AACATTCTCC AACAACTCAA TGAATCTCGA 
ATGACGTTGA GATCCGAGCA AAGAAGTCTG GAGGAAGCTG ACATAAAGAA AGAGAATCTT 
ACTGCTAAGC TGAAAACGGT GACCGAAGAC AATGGGAAGC TCATGAAGCA GGCCAGAGAG 
CTTCGAGATC TCAACGACTA TGAATTCGCT AGATTCCGTC AGCAGGAACA GGAGCTTACT 
GAGACTCTTC GGGCGACGCA GGACCAAATG GCTGACCTCC AGGAACAACT TACCGGAGTG 
GAAAAAATAA GAGCGTCTCT GAAATCTGAA AACGAGAGCC TTTCTGCCAG TGTTGAGGAG 
TTGAGTGTTG CTTCATTGAG AAACAAACAA GATGCTGACA ACTCTAAAAC TATGCTTTCC 
GAGGAACTGG CGCGTTTTGA AGAGACTGTG GACAAACTCC GACAAGAAAA GCTTGAAGCG 
TTGAGCATGG CCAACTCCAG AATCGATGCT CTTCGTTTGG AACACTCGGA AAGAGAGAAA 
ATGATGAAAT CCACGAATGC ACGCCTTCAG GCTGACCTTG ACGAAGAGAG GAACGAGAAA 
AAGCGCTTGA TGAATCTCTG GAATGAACTG AATGAAAAGT CCCTGAATGT CGACAAAGCT 
GTACATAAAA GCAACGAAGA ATTTCAGTCA ATGGGTTCTT GTCTTCAAAA TGCGAAAAGA 
CAAATTGAGC AGTTGGAAGT TGCGTGCAAG AGTAAGGACG ATATCATCAA ATTTCAAGAG 
GAGCAGAACA AACGAGCTGC CAATCTGATA AAGAATGAAA AGGCAATACG CGATCAGGCC 
TCAGCTCAAT TTGCCGAGAA GTTGGCAATT CTCAAGAATT CTCTAGCGGA AAAAGAGAAC 
GAAACAGTCA CCCTTAAAGA AAACTTCGCA AGTGTGGTAA TGAAGCATAA AGCCGAATTG 
GAGGAGAAAG AGCTGTTCCT TCAATCTAGA GTGGATCTGA TTCAACGTCT CGAGCACGAA 
GTTGCAGATC TTCGAGAGAA GGAAAGCGCC GAGATTAAGA AATCCACCTG GATGGGTGAG 
AGAATCAAAA TCTTCCAACA AGCTCCAATG AGAGGTTCGA GAATCAGATG CGGTTCGCCA 
GACAGCTTGC CAGACTTCCT CGCCGATAAT GGACCAACAA TTACCGAAGA AGAATTGCGA 
GCACAGCTTG CCACGTTCCC AAGAAAATCG ATTGCTCCTA GTGTGGACGA CAACGAATTC 
GATAAAGGAA CACCGATTGG ATTCAAGTCG AATATGTACG ATGGACGTGA GTCAATTTGC 
TCTTTGGATT TCTTCGACCG GTCTAGTCTT CAACGTTCGT CGATGCGATC GGAATCTATC 
CAATTGGCAT CGCCATCATC AGCTGGAGAG TTCAAGCAAC CATTCACACC ATCCGGAGTA 
ACCAAGGAAA GAGTTGGAGT GCTCACTGCC AGAAACGAGA AAGTGAAGCC ACACCTAAAA 
TGCTCGTACG CCTCTGAAGT TGGAAGTACG AATTCGCCAT CCGCAGACGA GGAGAATATC 
AAGAAGTCCA AGAAAAAGAA CCGTCGGGAT TCGATTTTTT CTGCTTTTTC GAGCAAAAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495654.1
CDS	1..2466
Translation	MSVSTSVVKSMLQLTNYFGSNFTPKTETISSLAELWERHGSMIASMMSCDTREFGTADKFYEVIFRKIATTSLSFMTDSLEPKLAASGDSLEICKFYALFLEYMRKDHSGMLTRALEQASSENRVDTAVLLDMFLFFDNVYSESTADDWWACLRRDDEDQEASSGFRTPKRNNYSLTSLQTPTATARRLRTASSTARRSPIAEAVDSPTMKFMRSERELKQSKARIFGLEQMLGDLEDDKTKLSAENRTLKLSNSDLKSDIAKLKGIAEEAQFRAEELSCDLEAKKDEVHNILQQLNESRMTLRSEQRSLEEADIKKENLTAKLKTVTEDNGKLMKQARELRDLNDYEFARFRQQEQELTETLRATQDQMADLQEQLTGVEKIRASLKSENESLSASVEELSVASLRNKQDADNSKTMLSEELARFEETVDKLRQEKLEALSMANSRIDALRLEHSEREKMMKSTNARLQADLDEERNEKKRLMNLWNELNEKSLNVDKAVHKSNEEFQSMGSCLQNAKRQIEQLEVACKSKDDIIKFQEEQNKRAANLIKNEKAIRDQASAQFAEKLAILKNSLAEKENETVTLKENFASVVMKHKAELEEKELFLQSRVDLIQRLEHEVADLREKESAEIKKSTWMGERIKIFQQAPMRGSRIRCGSPDSLPDFLADNGPTITEEELRAQLATFPRKSIAPSVDDNEFDKGTPIGFKSNMYDGRESICSLDFFDRSSLQRSSMRSESIQLASPSSAGEFKQPFTPSGVTKERVGVLTARNEKVKPHLKCSYASEVGSTNSPSADEENIKKSKKKNRRDSIFSAFSSKKQ

Target ORF information:

RefSeq Version	NM_063253.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063253.5

ORF Insert Sequence:

ATGAGCGTGA GCACATCAGT TGTGAAGTCA ATGCTACAAT TGACGAACTA TTTTGGCTCC 
AATTTCACAC CGAAGACGGA GACAATCAGC TCATTAGCCG AGCTTTGGGA GAGGCACGGG 
AGTATGATCG CCAGTATGAT GTCATGCGAC ACTCGGGAGT TTGGCACAGC TGACAAATTC 
TATGAAGTTA TCTTCCGCAA AATCGCCACA ACGAGTCTCA GCTTCATGAC CGATTCATTG 
GAACCGAAGC TTGCAGCGAG CGGAGATTCA CTGGAGATTT GCAAATTCTA CGCTCTGTTT 
CTCGAGTATA TGAGAAAGGA TCACTCGGGA ATGCTCACTC GCGCATTGGA GCAAGCGTCA 
TCAGAGAATC GCGTCGACAC AGCTGTGCTT CTTGATATGT TTTTGTTTTT CGACAACGTG 
TATTCGGAAA GTACCGCTGA CGACTGGTGG GCATGTCTTC GGAGAGATGA CGAAGATCAG 
GAAGCATCCA GTGGCTTCAG GACTCCGAAA AGGAACAACT ACAGCTTGAC ATCATTGCAA 
ACACCAACAG CTACTGCCCG GCGACTACGC ACAGCTTCCT CAACTGCTCG GCGAAGCCCT 
ATAGCGGAGG CTGTCGATTC CCCTACCATG AAGTTCATGC GAAGCGAGCG GGAGCTGAAG 
CAGTCTAAAG CGAGAATCTT TGGTTTGGAG CAAATGTTAG GAGACCTTGA AGATGACAAG 
ACCAAGTTAT CGGCTGAGAA CAGAACTTTG AAGCTTTCGA ATTCCGATCT GAAAAGCGAC 
ATCGCTAAAC TGAAAGGTAT CGCCGAGGAG GCACAATTTC GTGCTGAAGA ATTGAGCTGT 
GATCTCGAAG CCAAAAAGGA TGAAGTCCAC AACATTCTCC AACAACTCAA TGAATCTCGA 
ATGACGTTGA GATCCGAGCA AAGAAGTCTG GAGGAAGCTG ACATAAAGAA AGAGAATCTT 
ACTGCTAAGC TGAAAACGGT GACCGAAGAC AATGGGAAGC TCATGAAGCA GGCCAGAGAG 
CTTCGAGATC TCAACGACTA TGAATTCGCT AGATTCCGTC AGCAGGAACA GGAGCTTACT 
GAGACTCTTC GGGCGACGCA GGACCAAATG GCTGACCTCC AGGAACAACT TACCGGAGTG 
GAAAAAATAA GAGCGTCTCT GAAATCTGAA AACGAGAGCC TTTCTGCCAG TGTTGAGGAG 
TTGAGTGTTG CTTCATTGAG AAACAAACAA GATGCTGACA ACTCTAAAAC TATGCTTTCC 
GAGGAACTGG CGCGTTTTGA AGAGACTGTG GACAAACTCC GACAAGAAAA GCTTGAAGCG 
TTGAGCATGG CCAACTCCAG AATCGATGCT CTTCGTTTGG AACACTCGGA AAGAGAGAAA 
ATGATGAAAT CCACGAATGC ACGCCTTCAG GCTGACCTTG ACGAAGAGAG GAACGAGAAA 
AAGCGCTTGA TGAATCTCTG GAATGAACTG AATGAAAAGT CCCTGAATGT CGACAAAGCT 
GTACATAAAA GCAACGAAGA ATTTCAGTCA ATGGGTTCTT GTCTTCAAAA TGCGAAAAGA 
CAAATTGAGC AGTTGGAAGT TGCGTGCAAG AGTAAGGACG ATATCATCAA ATTTCAAGAG 
GAGCAGAACA AACGAGCTGC CAATCTGATA AAGAATGAAA AGGCAATACG CGATCAGGCC 
TCAGCTCAAT TTGCCGAGAA GTTGGCAATT CTCAAGAATT CTCTAGCGGA AAAAGAGAAC 
GAAACAGTCA CCCTTAAAGA AAACTTCGCA AGTGTGGTAA TGAAGCATAA AGCCGAATTG 
GAGGAGAAAG AGCTGTTCCT TCAATCTAGA GTGGATCTGA TTCAACGTCT CGAGCACGAA 
GTTGCAGATC TTCGAGAGAA GGAAAGCGCC GAGATTAAGA AATCCACCTG GATGGGTGAG 
AGAATCAAAA TCTTCCAACA AGCTCCAATG AGAGGTTCGA GAATCAGATG CGGTTCGCCA 
GACAGCTTGC CAGACTTCCT CGCCGATAAT GGACCAACAA TTACCGAAGA AGAATTGCGA 
GCACAGCTTG CCACGTTCCC AAGAAAATCG ATTGCTCCTA GTGTGGACGA CAACGAATTC 
GATAAAGGAA CACCGATTGG ATTCAAGTCG AATATGTACG ATGGACGTGA GTCAATTTGC 
TCTTTGGATT TCTTCGACCG GTCTAGTCTT CAACGTTCGT CGATGCGATC GGAATCTATC 
CAATTGGCAT CGCCATCATC AGCTGGAGAG TTCAAGCAAC CATTCACACC ATCCGGAGTA 
ACCAAGGAAA GAGTTGGAGT GCTCACTGCC AGAAACGAGA AAGTGAAGCC ACACCTAAAA 
TGCTCGTACG CCTCTGAAGT TGGAAGTACG AATTCGCCAT CCGCAGACGA GGAGAATATC 
AAGAAGTCCA AGAAAAAGAA CCGTCGGGAT TCGATTTTTT CTGCTTTTTC GAGCAAAAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lin-5 ( NM_063253.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495654.1 Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), mRNA.

Price & Availability↑

CloneID	OCa339497
Clone ID Related Accession (Same CDS sequence)	NM_063253.5 , NM_063253.6
Accession Version	NM_063253.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spindle apparatus protein lin-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063253.5.

ATGAGCGTGA GCACATCAGT TGTGAAGTCA ATGCTACAAT TGACGAACTA TTTTGGCTCC 
AATTTCACAC CGAAGACGGA GACAATCAGC TCATTAGCCG AGCTTTGGGA GAGGCACGGG 
AGTATGATCG CCAGTATGAT GTCATGCGAC ACTCGGGAGT TTGGCACAGC TGACAAATTC 
TATGAAGTTA TCTTCCGCAA AATCGCCACA ACGAGTCTCA GCTTCATGAC CGATTCATTG 
GAACCGAAGC TTGCAGCGAG CGGAGATTCA CTGGAGATTT GCAAATTCTA CGCTCTGTTT 
CTCGAGTATA TGAGAAAGGA TCACTCGGGA ATGCTCACTC GCGCATTGGA GCAAGCGTCA 
TCAGAGAATC GCGTCGACAC AGCTGTGCTT CTTGATATGT TTTTGTTTTT CGACAACGTG 
TATTCGGAAA GTACCGCTGA CGACTGGTGG GCATGTCTTC GGAGAGATGA CGAAGATCAG 
GAAGCATCCA GTGGCTTCAG GACTCCGAAA AGGAACAACT ACAGCTTGAC ATCATTGCAA 
ACACCAACAG CTACTGCCCG GCGACTACGC ACAGCTTCCT CAACTGCTCG GCGAAGCCCT 
ATAGCGGAGG CTGTCGATTC CCCTACCATG AAGTTCATGC GAAGCGAGCG GGAGCTGAAG 
CAGTCTAAAG CGAGAATCTT TGGTTTGGAG CAAATGTTAG GAGACCTTGA AGATGACAAG 
ACCAAGTTAT CGGCTGAGAA CAGAACTTTG AAGCTTTCGA ATTCCGATCT GAAAAGCGAC 
ATCGCTAAAC TGAAAGGTAT CGCCGAGGAG GCACAATTTC GTGCTGAAGA ATTGAGCTGT 
GATCTCGAAG CCAAAAAGGA TGAAGTCCAC AACATTCTCC AACAACTCAA TGAATCTCGA 
ATGACGTTGA GATCCGAGCA AAGAAGTCTG GAGGAAGCTG ACATAAAGAA AGAGAATCTT 
ACTGCTAAGC TGAAAACGGT GACCGAAGAC AATGGGAAGC TCATGAAGCA GGCCAGAGAG 
CTTCGAGATC TCAACGACTA TGAATTCGCT AGATTCCGTC AGCAGGAACA GGAGCTTACT 
GAGACTCTTC GGGCGACGCA GGACCAAATG GCTGACCTCC AGGAACAACT TACCGGAGTG 
GAAAAAATAA GAGCGTCTCT GAAATCTGAA AACGAGAGCC TTTCTGCCAG TGTTGAGGAG 
TTGAGTGTTG CTTCATTGAG AAACAAACAA GATGCTGACA ACTCTAAAAC TATGCTTTCC 
GAGGAACTGG CGCGTTTTGA AGAGACTGTG GACAAACTCC GACAAGAAAA GCTTGAAGCG 
TTGAGCATGG CCAACTCCAG AATCGATGCT CTTCGTTTGG AACACTCGGA AAGAGAGAAA 
ATGATGAAAT CCACGAATGC ACGCCTTCAG GCTGACCTTG ACGAAGAGAG GAACGAGAAA 
AAGCGCTTGA TGAATCTCTG GAATGAACTG AATGAAAAGT CCCTGAATGT CGACAAAGCT 
GTACATAAAA GCAACGAAGA ATTTCAGTCA ATGGGTTCTT GTCTTCAAAA TGCGAAAAGA 
CAAATTGAGC AGTTGGAAGT TGCGTGCAAG AGTAAGGACG ATATCATCAA ATTTCAAGAG 
GAGCAGAACA AACGAGCTGC CAATCTGATA AAGAATGAAA AGGCAATACG CGATCAGGCC 
TCAGCTCAAT TTGCCGAGAA GTTGGCAATT CTCAAGAATT CTCTAGCGGA AAAAGAGAAC 
GAAACAGTCA CCCTTAAAGA AAACTTCGCA AGTGTGGTAA TGAAGCATAA AGCCGAATTG 
GAGGAGAAAG AGCTGTTCCT TCAATCTAGA GTGGATCTGA TTCAACGTCT CGAGCACGAA 
GTTGCAGATC TTCGAGAGAA GGAAAGCGCC GAGATTAAGA AATCCACCTG GATGGGTGAG 
AGAATCAAAA TCTTCCAACA AGCTCCAATG AGAGGTTCGA GAATCAGATG CGGTTCGCCA 
GACAGCTTGC CAGACTTCCT CGCCGATAAT GGACCAACAA TTACCGAAGA AGAATTGCGA 
GCACAGCTTG CCACGTTCCC AAGAAAATCG ATTGCTCCTA GTGTGGACGA CAACGAATTC 
GATAAAGGAA CACCGATTGG ATTCAAGTCG AATATGTACG ATGGACGTGA GTCAATTTGC 
TCTTTGGATT TCTTCGACCG GTCTAGTCTT CAACGTTCGT CGATGCGATC GGAATCTATC 
CAATTGGCAT CGCCATCATC AGCTGGAGAG TTCAAGCAAC CATTCACACC ATCCGGAGTA 
ACCAAGGAAA GAGTTGGAGT GCTCACTGCC AGAAACGAGA AAGTGAAGCC ACACCTAAAA 
TGCTCGTACG CCTCTGAAGT TGGAAGTACG AATTCGCCAT CCGCAGACGA GGAGAATATC 
AAGAAGTCCA AGAAAAAGAA CCGTCGGGAT TCGATTTTTT CTGCTTTTTC GAGCAAAAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495654.1
CDS	2..2467
Translation	MSVSTSVVKSMLQLTNYFGSNFTPKTETISSLAELWERHGSMIASMMSCDTREFGTADKFYEVIFRKIATTSLSFMTDSLEPKLAASGDSLEICKFYALFLEYMRKDHSGMLTRALEQASSENRVDTAVLLDMFLFFDNVYSESTADDWWACLRRDDEDQEASSGFRTPKRNNYSLTSLQTPTATARRLRTASSTARRSPIAEAVDSPTMKFMRSERELKQSKARIFGLEQMLGDLEDDKTKLSAENRTLKLSNSDLKSDIAKLKGIAEEAQFRAEELSCDLEAKKDEVHNILQQLNESRMTLRSEQRSLEEADIKKENLTAKLKTVTEDNGKLMKQARELRDLNDYEFARFRQQEQELTETLRATQDQMADLQEQLTGVEKIRASLKSENESLSASVEELSVASLRNKQDADNSKTMLSEELARFEETVDKLRQEKLEALSMANSRIDALRLEHSEREKMMKSTNARLQADLDEERNEKKRLMNLWNELNEKSLNVDKAVHKSNEEFQSMGSCLQNAKRQIEQLEVACKSKDDIIKFQEEQNKRAANLIKNEKAIRDQASAQFAEKLAILKNSLAEKENETVTLKENFASVVMKHKAELEEKELFLQSRVDLIQRLEHEVADLREKESAEIKKSTWMGERIKIFQQAPMRGSRIRCGSPDSLPDFLADNGPTITEEELRAQLATFPRKSIAPSVDDNEFDKGTPIGFKSNMYDGRESICSLDFFDRSSLQRSSMRSESIQLASPSSAGEFKQPFTPSGVTKERVGVLTARNEKVKPHLKCSYASEVGSTNSPSADEENIKKSKKKNRRDSIFSAFSSKKQ

Target ORF information:

RefSeq Version	NM_063253.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spindle apparatus protein lin-5 (lin-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063253.6

ORF Insert Sequence:

ATGAGCGTGA GCACATCAGT TGTGAAGTCA ATGCTACAAT TGACGAACTA TTTTGGCTCC 
AATTTCACAC CGAAGACGGA GACAATCAGC TCATTAGCCG AGCTTTGGGA GAGGCACGGG 
AGTATGATCG CCAGTATGAT GTCATGCGAC ACTCGGGAGT TTGGCACAGC TGACAAATTC 
TATGAAGTTA TCTTCCGCAA AATCGCCACA ACGAGTCTCA GCTTCATGAC CGATTCATTG 
GAACCGAAGC TTGCAGCGAG CGGAGATTCA CTGGAGATTT GCAAATTCTA CGCTCTGTTT 
CTCGAGTATA TGAGAAAGGA TCACTCGGGA ATGCTCACTC GCGCATTGGA GCAAGCGTCA 
TCAGAGAATC GCGTCGACAC AGCTGTGCTT CTTGATATGT TTTTGTTTTT CGACAACGTG 
TATTCGGAAA GTACCGCTGA CGACTGGTGG GCATGTCTTC GGAGAGATGA CGAAGATCAG 
GAAGCATCCA GTGGCTTCAG GACTCCGAAA AGGAACAACT ACAGCTTGAC ATCATTGCAA 
ACACCAACAG CTACTGCCCG GCGACTACGC ACAGCTTCCT CAACTGCTCG GCGAAGCCCT 
ATAGCGGAGG CTGTCGATTC CCCTACCATG AAGTTCATGC GAAGCGAGCG GGAGCTGAAG 
CAGTCTAAAG CGAGAATCTT TGGTTTGGAG CAAATGTTAG GAGACCTTGA AGATGACAAG 
ACCAAGTTAT CGGCTGAGAA CAGAACTTTG AAGCTTTCGA ATTCCGATCT GAAAAGCGAC 
ATCGCTAAAC TGAAAGGTAT CGCCGAGGAG GCACAATTTC GTGCTGAAGA ATTGAGCTGT 
GATCTCGAAG CCAAAAAGGA TGAAGTCCAC AACATTCTCC AACAACTCAA TGAATCTCGA 
ATGACGTTGA GATCCGAGCA AAGAAGTCTG GAGGAAGCTG ACATAAAGAA AGAGAATCTT 
ACTGCTAAGC TGAAAACGGT GACCGAAGAC AATGGGAAGC TCATGAAGCA GGCCAGAGAG 
CTTCGAGATC TCAACGACTA TGAATTCGCT AGATTCCGTC AGCAGGAACA GGAGCTTACT 
GAGACTCTTC GGGCGACGCA GGACCAAATG GCTGACCTCC AGGAACAACT TACCGGAGTG 
GAAAAAATAA GAGCGTCTCT GAAATCTGAA AACGAGAGCC TTTCTGCCAG TGTTGAGGAG 
TTGAGTGTTG CTTCATTGAG AAACAAACAA GATGCTGACA ACTCTAAAAC TATGCTTTCC 
GAGGAACTGG CGCGTTTTGA AGAGACTGTG GACAAACTCC GACAAGAAAA GCTTGAAGCG 
TTGAGCATGG CCAACTCCAG AATCGATGCT CTTCGTTTGG AACACTCGGA AAGAGAGAAA 
ATGATGAAAT CCACGAATGC ACGCCTTCAG GCTGACCTTG ACGAAGAGAG GAACGAGAAA 
AAGCGCTTGA TGAATCTCTG GAATGAACTG AATGAAAAGT CCCTGAATGT CGACAAAGCT 
GTACATAAAA GCAACGAAGA ATTTCAGTCA ATGGGTTCTT GTCTTCAAAA TGCGAAAAGA 
CAAATTGAGC AGTTGGAAGT TGCGTGCAAG AGTAAGGACG ATATCATCAA ATTTCAAGAG 
GAGCAGAACA AACGAGCTGC CAATCTGATA AAGAATGAAA AGGCAATACG CGATCAGGCC 
TCAGCTCAAT TTGCCGAGAA GTTGGCAATT CTCAAGAATT CTCTAGCGGA AAAAGAGAAC 
GAAACAGTCA CCCTTAAAGA AAACTTCGCA AGTGTGGTAA TGAAGCATAA AGCCGAATTG 
GAGGAGAAAG AGCTGTTCCT TCAATCTAGA GTGGATCTGA TTCAACGTCT CGAGCACGAA 
GTTGCAGATC TTCGAGAGAA GGAAAGCGCC GAGATTAAGA AATCCACCTG GATGGGTGAG 
AGAATCAAAA TCTTCCAACA AGCTCCAATG AGAGGTTCGA GAATCAGATG CGGTTCGCCA 
GACAGCTTGC CAGACTTCCT CGCCGATAAT GGACCAACAA TTACCGAAGA AGAATTGCGA 
GCACAGCTTG CCACGTTCCC AAGAAAATCG ATTGCTCCTA GTGTGGACGA CAACGAATTC 
GATAAAGGAA CACCGATTGG ATTCAAGTCG AATATGTACG ATGGACGTGA GTCAATTTGC 
TCTTTGGATT TCTTCGACCG GTCTAGTCTT CAACGTTCGT CGATGCGATC GGAATCTATC 
CAATTGGCAT CGCCATCATC AGCTGGAGAG TTCAAGCAAC CATTCACACC ATCCGGAGTA 
ACCAAGGAAA GAGTTGGAGT GCTCACTGCC AGAAACGAGA AAGTGAAGCC ACACCTAAAA 
TGCTCGTACG CCTCTGAAGT TGGAAGTACG AATTCGCCAT CCGCAGACGA GGAGAATATC 
AAGAAGTCCA AGAAAAAGAA CCGTCGGGAT TCGATTTTTT CTGCTTTTTC GAGCAAAAAG 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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lin-5 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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