spd-5 cDNA ORF clone, Caenorhabditis elegans

The following spd-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spd-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa336644 NM_059138.5
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Caenorhabditis elegans Spindle-defective protein 5 (spd-5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
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OCa336644 NM_059138.4 Caenorhabditis elegans Spindle-defective protein 5 (spd-5), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa336644
    Clone ID Related Accession (Same CDS sequence) NM_059138.5 , NM_059138.4
    Accession Version NM_059138.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Spindle-defective protein 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059138.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
    CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC
    AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC
    CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA
    GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA
    GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG
    AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA
    ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG
    GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA
    TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC
    TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC
    AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT
    GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA
    ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT
    CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT
    GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT
    AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC
    ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT
    GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC
    GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG
    ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT
    CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA
    TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG
    AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG
    CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT
    CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG
    GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG
    TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG
    TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC
    CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT
    GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG
    AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA
    GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT
    AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC
    TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC
    ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT
    ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC
    AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA
    GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT
    AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT
    GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT
    TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG
    AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA
    GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG
    AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT
    GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC
    GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC
    GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG
    CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC
    GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG
    AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC
    GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG
    GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC
    CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC
    AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC
    ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA
    ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA
    TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA
    CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA
    ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_491539.1
    CDS83..3679
    Translation

    Target ORF information:

    RefSeq Version NM_059138.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Spindle-defective protein 5 (spd-5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059138.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
    CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC
    AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC
    CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA
    GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA
    GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG
    AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA
    ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG
    GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA
    TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC
    TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC
    AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT
    GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA
    ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT
    CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT
    GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT
    AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC
    ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT
    GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC
    GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG
    ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT
    CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA
    TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG
    AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG
    CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT
    CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG
    GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG
    TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG
    TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC
    CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT
    GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG
    AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA
    GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT
    AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC
    TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC
    ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT
    ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC
    AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA
    GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT
    AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT
    GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT
    TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG
    AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA
    GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG
    AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT
    GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC
    GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC
    GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG
    CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC
    GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG
    AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC
    GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG
    GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC
    CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC
    AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC
    ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA
    ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA
    TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA
    CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA
    ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa336644
    Clone ID Related Accession (Same CDS sequence) NM_059138.5 , NM_059138.4
    Accession Version NM_059138.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Spindle-defective protein 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059138.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
    CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC
    AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC
    CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA
    GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA
    GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG
    AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA
    ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG
    GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA
    TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC
    TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC
    AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT
    GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA
    ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT
    CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT
    GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT
    AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC
    ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT
    GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC
    GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG
    ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT
    CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA
    TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG
    AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG
    CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT
    CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG
    GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG
    TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG
    TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC
    CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT
    GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG
    AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA
    GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT
    AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC
    TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC
    ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT
    ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC
    AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA
    GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT
    AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT
    GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT
    TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG
    AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA
    GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG
    AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT
    GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC
    GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC
    GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG
    CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC
    GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG
    AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC
    GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG
    GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC
    CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC
    AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC
    ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA
    ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA
    TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA
    CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA
    ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_491539.1
    CDS1..3597
    Translation

    Target ORF information:

    RefSeq Version NM_059138.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Spindle-defective protein 5 (spd-5), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_059138.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
    CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC
    AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC
    CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA
    GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA
    GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG
    AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA
    ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG
    GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA
    TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC
    TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC
    AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT
    GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA
    ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT
    CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT
    GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT
    AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC
    ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT
    GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC
    GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG
    ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT
    CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA
    TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG
    AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG
    CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT
    CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG
    GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG
    TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG
    TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC
    CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT
    GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG
    AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA
    GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT
    AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC
    TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC
    ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT
    ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC
    AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA
    GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT
    AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT
    GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT
    TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG
    AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA
    GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG
    AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT
    GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC
    GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC
    GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG
    CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC
    GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG
    AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC
    GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG
    GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC
    CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC
    AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC
    ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA
    ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA
    TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA
    CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA
    ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.