spd-5 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	spd-5
Entrez Gene ID	172155
Full Name	Spindle-defective protein 5
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_059138.5	NP_491539.1	Spindle-defective protein 5
NM_059138.4	NP_491539.1	Spindle-defective protein 5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recruitment of the scaffold component SPD-5 occurs throughout the pericentriolar material (PCM). Incorporation of additional PCM subunits is therefore not limited to specific nucleation sites near centrioles.
Title: Isotropic incorporation of SPD-5 underlies centrosome assembly in C. elegans.

The following spd-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spd-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336644	NM_059138.5 Latest version!	Caenorhabditis elegans Spindle-defective protein 5 (spd-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa336644	NM_059138.4	Caenorhabditis elegans Spindle-defective protein 5 (spd-5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spd-5 ( NM_059138.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491539.1 Caenorhabditis elegans Spindle-defective protein 5 (spd-5), mRNA.

Price & Availability↑

CloneID	OCa336644
Clone ID Related Accession (Same CDS sequence)	NM_059138.5 , NM_059138.4
Accession Version	NM_059138.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spindle-defective protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_059138.4.

ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC 
AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC 
CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA 
GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA 
GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG 
AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA 
ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG 
GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA 
TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC 
TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC 
AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT 
GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA 
ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT 
CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT 
GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT 
AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC 
ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT 
GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC 
GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG 
ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT 
CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA 
TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG 
AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG 
CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT 
CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG 
GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG 
TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG 
TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC 
CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT 
GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG 
AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA 
GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT 
AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC 
TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC 
ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT 
ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC 
AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA 
GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT 
AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT 
GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT 
TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG 
AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA 
GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG 
AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT 
GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC 
GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC 
GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG 
CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC 
GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG 
AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC 
GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG 
GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC 
CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC 
AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC 
ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA 
ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA 
TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA 
CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA 
ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491539.1
CDS	83..3679
Translation	MEDNSVLNEDSNLEHVEGQPRRSMSQPVLNVEGDKRTSSTSATQQQVLSGAFSSADVRSIPIIQTWEENKALKTKITILRGELQMYQRRYSEAKEASQKRVKEVMDDYVDLKLGQENVQEKMEQYKLMEEDLLAMQSRIETSEDNFARQMKEFEAQKHAMEERIKELELSATDANNTTVGSFRGTLDDILKKNDPDFTLTSGYEERKINDLEAKLLSEIDKVAELEDHIQQLRQELDDQSARLADSENVRAQLEAATGQGILGAAGNAMVPNSTFMIGNGRESQTRDQLNYIDDLETKLADAKKENDKARQALVEYMNKCSKLEHEIRTMVKNSTFDSSSMLLGGQTSDELKIQIGKVNGELNVLRAENRELRIRCDQLTGGDGNLSISLGQSRLMAGIATNDVDSIGQGNETGGTSMRILPRESQLDDLEESKLPLMDTSSAVRNQQQFASMWEDFESVKDSLQNNHNDTLEGSFNSSMPPPGRDATQSFLSQKSFKNSPIVMQKPKSLHLHLKSHQSEGAGEQIQNNSFSTKTASPHVSQSHIPILHDMQQILDSSAMFLEGQHDVAVNVEQMQEKMSQIREALARLFERLKSSAALFEEILERMGSSDPNADKIKKMKLAFETSINDKLNVSAILEAAEKDLHNMSLNFSILEKSIVSQAAEASRRFTIAPDAEDVASSSLLNASYSPLFKFTSNSDIVEKLQNEVSELKNELEMARTRDMRSPLNGSSGRLSDVQINTNRMFEDLEVSEATLQKAKEENSTLKSQFAELEANLHQVNSKLGEVRCELNEALARVDGEQETRVKAENALEEARQLISSLKHEENELKKTITDMGMRLNEAKKSDEFLKSELSTALEEEKKSQNLADELSEELNGWRMRTKEAENKVEHASSEKSEMLERIVHLETEMEKLSTSEIAADYCSTKMTERKKEIELAKYREDFENAAIVGLERISKEISELTKKTLKAKIIPSNISSIQLVCDELCRRLSREREQQHEYAKVMRDVNEKIEKLQLEKDALEHELKMMSSNNENVPPVGTSVSGMPTKTSNQKCAQPHYTSPTRQLLHESTMAVDAIVQKLKKTHNMSGMGPELKETIGNVINESRVLRDFLHQKLILFKGIDMSNWKNETVDQLITDLGQLHQDNLMLEEQIKKYKKELKLTKSAIPTLGVEFQDRIKTEIGKIATDMGGAVKEIRKK

Target ORF information:

RefSeq Version	NM_059138.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spindle-defective protein 5 (spd-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059138.5

ORF Insert Sequence:

ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC 
AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC 
CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA 
GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA 
GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG 
AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA 
ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG 
GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA 
TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC 
TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC 
AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT 
GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA 
ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT 
CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT 
GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT 
AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC 
ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT 
GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC 
GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG 
ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT 
CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA 
TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG 
AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG 
CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT 
CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG 
GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG 
TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG 
TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC 
CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT 
GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG 
AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA 
GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT 
AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC 
TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC 
ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT 
ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC 
AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA 
GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT 
AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT 
GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT 
TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG 
AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA 
GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG 
AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT 
GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC 
GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC 
GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG 
CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC 
GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG 
AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC 
GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG 
GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC 
CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC 
AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC 
ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA 
ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA 
TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA 
CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA 
ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spd-5 ( NM_059138.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_491539.1 Caenorhabditis elegans Spindle-defective protein 5 (spd-5), partial mRNA.

Price & Availability↑

CloneID	OCa336644
Clone ID Related Accession (Same CDS sequence)	NM_059138.5 , NM_059138.4
Accession Version	NM_059138.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spindle-defective protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_059138.3. COMPLETENESS: incomplete on both ends.

ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC 
AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC 
CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA 
GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA 
GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG 
AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA 
ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG 
GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA 
TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC 
TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC 
AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT 
GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA 
ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT 
CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT 
GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT 
AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC 
ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT 
GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC 
GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG 
ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT 
CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA 
TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG 
AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG 
CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT 
CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG 
GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG 
TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG 
TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC 
CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT 
GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG 
AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA 
GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT 
AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC 
TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC 
ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT 
ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC 
AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA 
GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT 
AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT 
GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT 
TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG 
AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA 
GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG 
AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT 
GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC 
GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC 
GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG 
CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC 
GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG 
AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC 
GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG 
GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC 
CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC 
AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC 
ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA 
ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA 
TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA 
CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA 
ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_491539.1
CDS	1..3597
Translation	MEDNSVLNEDSNLEHVEGQPRRSMSQPVLNVEGDKRTSSTSATQQQVLSGAFSSADVRSIPIIQTWEENKALKTKITILRGELQMYQRRYSEAKEASQKRVKEVMDDYVDLKLGQENVQEKMEQYKLMEEDLLAMQSRIETSEDNFARQMKEFEAQKHAMEERIKELELSATDANNTTVGSFRGTLDDILKKNDPDFTLTSGYEERKINDLEAKLLSEIDKVAELEDHIQQLRQELDDQSARLADSENVRAQLEAATGQGILGAAGNAMVPNSTFMIGNGRESQTRDQLNYIDDLETKLADAKKENDKARQALVEYMNKCSKLEHEIRTMVKNSTFDSSSMLLGGQTSDELKIQIGKVNGELNVLRAENRELRIRCDQLTGGDGNLSISLGQSRLMAGIATNDVDSIGQGNETGGTSMRILPRESQLDDLEESKLPLMDTSSAVRNQQQFASMWEDFESVKDSLQNNHNDTLEGSFNSSMPPPGRDATQSFLSQKSFKNSPIVMQKPKSLHLHLKSHQSEGAGEQIQNNSFSTKTASPHVSQSHIPILHDMQQILDSSAMFLEGQHDVAVNVEQMQEKMSQIREALARLFERLKSSAALFEEILERMGSSDPNADKIKKMKLAFETSINDKLNVSAILEAAEKDLHNMSLNFSILEKSIVSQAAEASRRFTIAPDAEDVASSSLLNASYSPLFKFTSNSDIVEKLQNEVSELKNELEMARTRDMRSPLNGSSGRLSDVQINTNRMFEDLEVSEATLQKAKEENSTLKSQFAELEANLHQVNSKLGEVRCELNEALARVDGEQETRVKAENALEEARQLISSLKHEENELKKTITDMGMRLNEAKKSDEFLKSELSTALEEEKKSQNLADELSEELNGWRMRTKEAENKVEHASSEKSEMLERIVHLETEMEKLSTSEIAADYCSTKMTERKKEIELAKYREDFENAAIVGLERISKEISELTKKTLKAKIIPSNISSIQLVCDELCRRLSREREQQHEYAKVMRDVNEKIEKLQLEKDALEHELKMMSSNNENVPPVGTSVSGMPTKTSNQKCAQPHYTSPTRQLLHESTMAVDAIVQKLKKTHNMSGMGPELKETIGNVINESRVLRDFLHQKLILFKGIDMSNWKNETVDQLITDLGQLHQDNLMLEEQIKKYKKELKLTKSAIPTLGVEFQDRIKTEIGKIATDMGGAVKEIRKK

Target ORF information:

RefSeq Version	NM_059138.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spindle-defective protein 5 (spd-5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_059138.4

ORF Insert Sequence:

ATGGAGGATA ATTCTGTGCT CAACGAGGAC TCCAATCTCG AGCATGTCGA GGGCCAGCCA 
CGAAGATCTA TGTCGCAACC AGTTCTGAAT GTTGAAGGAG ACAAAAGAAC GTCTTCGACC 
AGTGCCACTC AACAGCAAGT ATTGTCAGGA GCATTTTCGT CTGCTGATGT TCGTAGCATC 
CCCATCATCC AAACATGGGA AGAAAACAAA GCGCTTAAAA CAAAAATAAC TATCCTGAGA 
GGAGAACTGC AAATGTATCA GCGCCGGTAT AGTGAGGCAA AGGAAGCGTC GCAGAAGAGA 
GTTAAGGAAG TTATGGACGA TTATGTCGAT CTCAAATTAG GCCAAGAAAA TGTTCAAGAG 
AAAATGGAGC AATACAAACT GATGGAAGAG GATTTGCTAG CAATGCAATC GAGAATTGAA 
ACTTCCGAAG ACAATTTTGC CAGACAGATG AAAGAATTTG AGGCACAAAA GCATGCAATG 
GAGGAAAGAA TCAAGGAACT GGAATTGTCC GCTACTGATG CAAACAACAC AACTGTCGGA 
TCTTTTCGTG GAACTCTTGA TGACATTCTG AAGAAAAACG ATCCAGATTT CACATTAACC 
TCTGGTTATG AAGAAAGAAA GATCAACGAC CTGGAGGCAA AGCTCCTCTC TGAGATCGAC 
AAGGTAGCTG AGCTGGAAGA TCACATTCAG CAGCTCCGTC AAGAACTTGA CGACCAATCT 
GCAAGGCTTG CCGATTCAGA AAATGTTCGC GCTCAGCTTG AAGCGGCCAC TGGACAAGGA 
ATCCTCGGAG CTGCTGGAAA CGCTATGGTT CCAAATTCAA CGTTCATGAT CGGGAACGGT 
CGTGAATCAC AGACGCGAGA CCAGCTCAAT TACATTGATG ATCTTGAAAC GAAGTTAGCT 
GATGCGAAGA AGGAAAATGA TAAGGCTCGT CAGGCACTCG TTGAATACAT GAACAAGTGT 
AGCAAGCTTG AGCACGAGAT TCGAACCATG GTTAAAAATT CGACATTTGA TTCTTCTTCC 
ATGTTGCTTG GTGGTCAGAC ATCCGACGAG CTTAAAATCC AAATAGGAAA GGTGAACGGT 
GAATTGAATG TGTTGAGAGC TGAAAATCGA GAACTCCGCA TTCGTTGTGA TCAATTGACC 
GGAGGCGATG GAAATCTTAG CATTTCACTG GGACAAAGTC GACTGATGGC TGGTATTGCG 
ACTAATGATG TCGATAGCAT TGGACAAGGA AATGAGACTG GCGGAACATC GATGCGCATT 
CTTCCCAGAG AAAGTCAATT AGATGATCTT GAAGAAAGCA AATTGCCGCT CATGGATACA 
TCATCTGCTG TTCGAAATCA GCAACAATTT GCCTCCATGT GGGAAGATTT CGAGTCAGTG 
AAAGACAGCC TGCAGAACAA CCATAATGAC ACCCTTGAAG GTTCCTTCAA TTCGTCAATG 
CCACCACCTG GTAGAGATGC AACTCAATCG TTCCTCAGTC AGAAGTCTTT CAAGAATTCT 
CCGATTGTAA TGCAGAAACC AAAATCACTT CATCTTCACC TGAAGAGCCA TCAATCCGAG 
GGAGCTGGCG AACAAATACA GAATAATTCG TTTTCTACGA AGACTGCTTC TCCTCACGTG 
TCACAAAGTC ACATTCCAAT TCTGCACGAT ATGCAACAAA TTCTGGATTC TTCTGCAATG 
TTCCTTGAAG GCCAGCATGA TGTAGCTGTA AATGTTGAGC AAATGCAAGA GAAAATGTCC 
CAGATCAGAG AGGCTCTTGC TCGACTTTTC GAAAGACTTA AATCTTCTGC TGCTCTTTTT 
GAAGAGATTC TTGAAAGGAT GGGATCCAGT GATCCAAATG CGGATAAGAT CAAGAAAATG 
AAGCTCGCAT TTGAAACAAG TATTAACGAC AAGCTCAATG TATCTGCCAT CTTGGAAGCA 
GCGGAGAAGG ATCTTCATAA TATGTCACTC AATTTCTCAA TTCTCGAGAA GTCGATAGTT 
AGTCAGGCTG CGGAAGCTTC GAGAAGATTC ACTATTGCAC CCGATGCTGA AGATGTCGCC 
TCATCTTCTC TTCTCAATGC CTCGTATAGT CCACTCTTCA AGTTTACAAG CAATTCAGAC 
ATAGTGGAAA AGCTTCAGAA TGAGGTATCG GAGTTGAAAA ATGAATTGGA GATGGCTCGT 
ACACGAGATA TGAGATCTCC ATTGAACGGT TCGTCTGGAA GATTGTCGGA TGTGCAGATC 
AACACCAATC GCATGTTTGA AGATCTCGAA GTATCTGAAG CAACGCTACA AAAGGCTAAA 
GAGGAAAATT CTACATTGAA AAGTCAATTT GCTGAACTCG AAGCCAATCT CCATCAAGTT 
AATTCTAAGC TTGGAGAAGT GCGATGCGAG CTCAATGAAG CGTTGGCTCG TGTGGATGGT 
GAACAGGAGA CTCGTGTCAA AGCTGAAAAT GCATTGGAAG AGGCTCGTCA GCTCATCAGT 
TCTCTAAAAC ATGAGGAAAA TGAGCTCAAG AAAACCATTA CTGATATGGG GATGCGATTG 
AATGAAGCAA AGAAGAGCGA TGAATTCTTG AAAAGCGAGT TATCTACCGC GCTGGAGGAA 
GAAAAAAAAT CCCAAAATCT AGCAGATGAG TTGAGTGAAG AATTGAATGG GTGGAGAATG 
AGAACAAAAG AGGCGGAAAA TAAAGTTGAA CATGCTTCAT CCGAGAAATC TGAAATGCTT 
GAAAGAATTG TTCATTTGGA GACTGAGATG GAGAAGTTGT CGACTTCAGA AATTGCGGCC 
GACTATTGTT CTACGAAGAT GACAGAAAGA AAGAAGGAAA TTGAGTTGGC GAAGTATCGC 
GAAGATTTTG AGAATGCCGC TATTGTAGGA TTGGAACGTA TCAGTAAGGA GATATCTGAG 
CTAACCAAGA AGACTTTGAA AGCCAAGATT ATCCCAAGCA ACATCTCATC GATTCAACTC 
GTTTGCGATG AACTCTGTCG TCGTCTTTCC AGGGAACGAG AGCAACAGCA TGAATACGCG 
AAAGTTATGA GAGACGTCAA TGAAAAAATT GAAAAGCTTC AATTAGAGAA AGATGCTCTC 
GAACATGAGC TGAAAATGAT GAGCTCTAAT AACGAGAATG TCCCACCAGT TGGAACATCG 
GTCAGTGGCA TGCCTACTAA AACATCGAAT CAAAAGTGTG CTCAACCTCA TTACACGAGC 
CCTACTCGAC AATTGCTCCA CGAATCAACT ATGGCTGTCG ACGCAATTGT TCAGAAACTC 
AAGAAGACTC ACAATATGTC AGGAATGGGT CCAGAGCTTA AGGAGACGAT TGGCAACGTC 
ATCAATGAAT CCAGAGTTCT TCGCGATTTC CTCCATCAGA AGCTCATTCT ATTCAAAGGA 
ATCGATATGT CCAATTGGAA GAACGAGACC GTCGATCAAC TGATCACAGA TCTCGGACAA 
TTGCATCAGG ATAACTTGAT GCTTGAGGAG CAAATCAAGA AGTACAAAAA GGAACTGAAA 
CTGACAAAAT CCGCCATCCC GACACTTGGT GTCGAATTTC AAGACAGAAT CAAAACGGAA 
ATTGGCAAAA TCGCAACTGA TATGGGAGGA GCTGTGAAGG AAATTCGCAA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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spd-5 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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