tol-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	tol-1
Entrez Gene ID	171635
Full Name	TOLl (Drosophila) family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025812.4	NP_001020983.1	TIR domain-containing protein
NM_001025812.3	NP_001020983.1	TOLl (Drosophila) family

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Toll-7	NP_523797.1
Anopheles gambiae (African malaria mosquito)	TOLL7	XP_320221.4
Caenorhabditis elegans (roundworm)	tol-1	NP_001020983.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that pathologies in tol-1(-) mutant nematodes are indistinguishable from wild type animals, indicating that nematodes do not employ this orthologue for detection of Coxiella burnetii
Title: Analysis of the Caenorhabditis elegans innate immune response to Coxiella burnetii.

For pathogen avoidance TOL-1 signaling is required in the chemosensory BAG neurons, where it regulates gene expression and is necessary for their chemosensory function. TOL-1 acts together with many conserved components of TLR signaling.
Title: Toll-like Receptor Signaling Promotes Development and Function of Sensory Neurons Required for a C.?elegans Pathogen-Avoidance Behavior.

The results indicate that TOL-1 has a direct role in defence response to certain Gram-negative bacteria and indicate that part of the TLR-mediated immunity might be evolutionarily conserved.
Title: A conserved Toll-like receptor is required for Caenorhabditis elegans innate immunity.

The following tol-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tol-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa336436	NM_001025812.4 Latest version!	Caenorhabditis elegans TIR domain-containing protein (tol-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa336436	NM_001025812.3	Caenorhabditis elegans TOLl (Drosophila) family (tol-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tol-1 ( NM_001025812.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001020983.1 Caenorhabditis elegans TIR domain-containing protein (tol-1), mRNA.

Price & Availability↑

CloneID	OCa336436
Clone ID Related Accession (Same CDS sequence)	NM_001025812.4 , NM_001025812.3
Accession Version	NM_001025812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	TIR domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_001025812.3.

ATGAGGAGAA AAATGAAGTT ATTCCTATTT TTATTATTAG TAATTAATAT ATGTCGGTCG 
GCCGCTGCTA ACGGTGACGA ATGCCCGAAA TTCTGTAAAT GTGCTCCGGA TCCGGTGCAG 
CCGACGTCTA AACTCCTATT ATGCGACTAT TCTTCGAAAA ACACGACAAT TACACCTATT 
GCGTCGTCGA ATTATGATCA GGTTGCTAAT ATTCGATCAC TATTCATATC TTGTGATAAT 
AATAATTTCC AATTTCCGGA TGCCTACTTC AAGTCGTTAA CCGCGTTGCA TCATCTGCGG 
ATAGTGGGAT GCGAGACCAC ACATTTCTCT GTGAAATTGT TCGAAGATTT GGCCGCTTTG 
AGAAGATTGG AACTCGATCA GATCTCCACC GCCTCAACCT CTTTCGAAAT GACCGAAGAC 
GTCCTAATGC CGTTGGCTCG TCTCGAAAAG TTTTCCCTCA CGAGATCACG GAATATCGAG 
CTTCCACAGC GACTTTTGTG CTCTCTGCCG CATTTACAGG TTCTGAATAT CTCGTCAAAT 
GAGCTTCCAT CACTGCGAAG AGAGGAATCG TGTGTCGCTC AGCAGCTTCT GATCGTCGAT 
TTGTCTAGAA ATCGGCTCAC CAACATCGAG CAATTCCTAC GTGGCATCCC GGCAATCCGG 
CAAATTTCGG TGGCTTACAA CTCGATCGCC GAGCTCGATT TATCGCTGGC AACTCCATTT 
CTACAACAAC TCGATGCTGA AGCCAATCGA ATCGTCGACT TGACGTCACT TCCAGGCACT 
GTTGTACACG TGAATTTGGC TGGAAACGCG CTGAAAAGGG TGCCGGATGC GGTAGCCGAG 
TTGGCGAGTC TTGTGGCGTT AAATGTGTCG AGAAATGAGA TTGAAGCCGG AAATTCGTCG 
GTTTTTTCTT CCCCAGAACT CGAAATGCTC GACGCCTCTT ACAACAAATT GGACAGTCTG 
CCCGTCGAAT GGCTTCAAAA ATGCGAAAAA CGCATTGCTC ACCTCCATTT GGAGCACAAT 
TCGATTGAGC AGTTGACTGG TGGAGTGCTG GCGAATGCGA CTAATTTACA GACGTTGGAC 
CTTTCATCGA ATCAACTTCG AGTTTTTCGA GACGAAGTAC TTCCGGAGAA TTCGAAAATT 
GGAAATTTGA GACTTTCCAA CAACTCTTTG GAGCTCCTCG AGCCGTCGAG CCTGAGCGGC 
TTGAAATTGG AATCCCTTGA TCTGAGTCAT AATAAGCTGA CAGAAGTGCC CGCCGCAATT 
GGAAAAGTCG AGCAGCTGAA AAAAGTGGAT TTGAGCCATA ATAGAATTGC GAAGGTTTAT 
CAATATGTGC TCAATAAGAT TAAGCAATTG CATACTGTTG ATTTGTCGAA TAATCAGTTG 
CAAAGTATCG GCCCCTACAT CTTCTCCGAC AGTTCTGAAC TTCATTCCCT GGACGTGTCG 
AATAATGAGA TTTCACTGCT GTTCAAGGAC GCTTTTGCGA GATGCCCAAA GCTGAGGAAA 
ATTTCGATGA AAATGAATAA AATTAAATCC CTCGACGAAG GTCTCACAGA AGCTTCCGGC 
CTCCGACGTC TCGACGTATC TCATAACGAG ATCCTCGTGC TGAAATGGTC GGCTTTACCT 
GAAAACTTGG AGATTCTCAA CGCTGATAAC AATGATATCA ATCTCCTGAC CGCCGCCTCA 
ATGTCCCCAA GCACCGCAAA CTTGAAGTCC GTTTCGCTTT CCAACAACGG CATCACCATA 
ATGAATGCGG ACCAGATTCC GAATTCGCTC GAGTCGCTGG ACGTGTCGAA TAATCGACTT 
GCAAAGCTCG GGAAGACAGC GTTGGCCGCG AAATCTCAGT TGAGAAGGCT CAACTTGAAG 
GGCAATCTGC TTACCGTAGT GGCCACCGAG TCGATGAAAG TCGTAGAGGC TGTGCATCCG 
TTGAAAGTGG AAATCTCGGA GAATCCTCTG ATCTGTGATT GTCAGATGGG ATGGATGATT 
GGTGGAGCGA AGCCAAAGGT TCTCATTCAG GACTCTGAAA CCGCAAGCTG TTCCCATGCC 
GTTGATGGGC ATCAGATCCA GATTCAAAGT CTCAGCAAGA AGGATCTACT GTGCCCATAC 
AAAAGTGTAT GTGAGCCGGA ATGTATCTGC TGTCAATACG GAAATTGCGA TTGCAAATCC 
GTATGCCCCG CCAATTGCCG ATGCTTCAGA GATGATCAGT TTAATATCAA CATTGTCAGA 
TGCCACGGGA ACTCATCAAT GGTGCCCAAA AGAGAATTCG TGGTCTCCGA GCTCCCGGTC 
TCTGCGACAG AGATCATTCT GAGCGGAGTC ACCCTTCCAC AGCTCCGAAC TCACAGCTTC 
ATCGGAAGAC TTCGTCTCCA GAGGCTTCAT ATCAATGGAA CCGGGCTCCG ATCCATCCAA 
CCGAAGGCTT TCCATACTCT TCCAGCACTG AAGACGCTGG ATTTGTCGGA TAACTCGTTG 
ATCTCGCTGA GCGGGGAGGA ATTTCTAAAG TGTGGAGAAG TCTCGCAGCT TTTCCTCAAT 
GGAAATCGAT TTTCCACGCT ATCCCGTGGA ATCTTCGAGA AGCTTCCGAA CTTGAAATAT 
CTGACACTTC ATAACAACTC CCTCGAAGAC ATCCCTCAGG TTCTTCACTC GACGGCGCTC 
TCCAAGATCT CCCTGTCATC GAACCCCTTG AGATGCGACT GCTCGGGAGG ATCCCAACAG 
CACCTTCACC ATCGTCGTGA CCCAAAAGCT CATCCATTCT GGGAGCATAA TGCGGCCGAG 
TGGTTCTCGT TGCATCGGCA TCTTGTCGTT GATTTCCCCA AGGTTGAATG CTGGGAGAAC 
GTGACGAAGG CCTTCCTGAC GAACGATACG ACAGTGCTGA GCGCCTATCC ACCTAATATG 
GGAAATGACG TCTTTGTGAT GCCTATTGAA GAATTCCTGC GCGACTACAA CTCAACAATC 
TGTGTTCCAT TCTCATCTGG ATTCTTTGGA CAAGACCCTC AGAATAGTAT ACTCTTTGTA 
ATAATAACTA TATCGATTGC TGTTCTCCTC TGTGTCCTCG TTATTCTCGC AATTTCATTT 
ATTCGAAAAT CTCACGACGC AATCAATCAA CGAAGATACA AAGCATCATC TCTAAATTGT 
TCAACATCAG CCGGCTCGTC GCCTCTTCCG GTTCCGCTGT TGAGTTATCA CGCATTTGTG 
AGCTATTCGA AGAAGGACGA GAAAATGGTG ATTGATCAAT TGTGTCGACC GCTGGAAGAT 
GAAGATTATC AGTTGTGCCT GTTGCATCGG GATGGGCCGA CTTATTGCTC GAATTTGCAC 
GCGATATCTG ATGAACTCAT AGCTCAGATG GACTCGTCGC AATGCTTGAT TCTTGTGTTG 
ACTAAACACT TTTTGGAGAA CGAGTGGAAA ACGCTGCAGA TTAAGACCTC CCACCAACTA 
TTCGCCAAAA ACCGTGCAAA ACGAGTGATC GCCGTGCTCG GCGACGGTGT GGACGCGAAT 
CTGCTGGACG ATGAGCTCGG ACAGATTCTA CGGAAGCACA CGAGAATCGA GATGCGGAGC 
CATTTATTCT GGACACTTTT GCACTCATCA CTTCCATCAC GACTTCCATT ACCATCGAAT 
AGTGGCGATG ATTCGTCTCA ACTATATTCG GATATCTATG GAATTGTGCC TTCCGATGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020983.1
CDS	20..3685
Translation	MRRKMKLFLFLLLVINICRSAAANGDECPKFCKCAPDPVQPTSKLLLCDYSSKNTTITPIASSNYDQVANIRSLFISCDNNNFQFPDAYFKSLTALHHLRIVGCETTHFSVKLFEDLAALRRLELDQISTASTSFEMTEDVLMPLARLEKFSLTRSRNIELPQRLLCSLPHLQVLNISSNELPSLRREESCVAQQLLIVDLSRNRLTNIEQFLRGIPAIRQISVAYNSIAELDLSLATPFLQQLDAEANRIVDLTSLPGTVVHVNLAGNALKRVPDAVAELASLVALNVSRNEIEAGNSSVFSSPELEMLDASYNKLDSLPVEWLQKCEKRIAHLHLEHNSIEQLTGGVLANATNLQTLDLSSNQLRVFRDEVLPENSKIGNLRLSNNSLELLEPSSLSGLKLESLDLSHNKLTEVPAAIGKVEQLKKVDLSHNRIAKVYQYVLNKIKQLHTVDLSNNQLQSIGPYIFSDSSELHSLDVSNNEISLLFKDAFARCPKLRKISMKMNKIKSLDEGLTEASGLRRLDVSHNEILVLKWSALPENLEILNADNNDINLLTAASMSPSTANLKSVSLSNNGITIMNADQIPNSLESLDVSNNRLAKLGKTALAAKSQLRRLNLKGNLLTVVATESMKVVEAVHPLKVEISENPLICDCQMGWMIGGAKPKVLIQDSETASCSHAVDGHQIQIQSLSKKDLLCPYKSVCEPECICCQYGNCDCKSVCPANCRCFRDDQFNINIVRCHGNSSMVPKREFVVSELPVSATEIILSGVTLPQLRTHSFIGRLRLQRLHINGTGLRSIQPKAFHTLPALKTLDLSDNSLISLSGEEFLKCGEVSQLFLNGNRFSTLSRGIFEKLPNLKYLTLHNNSLEDIPQVLHSTALSKISLSSNPLRCDCSGGSQQHLHHRRDPKAHPFWEHNAAEWFSLHRHLVVDFPKVECWENVTKAFLTNDTTVLSAYPPNMGNDVFVMPIEEFLRDYNSTICVPFSSGFFGQDPQNSILFVIITISIAVLLCVLVILAISFIRKSHDAINQRRYKASSLNCSTSAGSSPLPVPLLSYHAFVSYSKKDEKMVIDQLCRPLEDEDYQLCLLHRDGPTYCSNLHAISDELIAQMDSSQCLILVLTKHFLENEWKTLQIKTSHQLFAKNRAKRVIAVLGDGVDANLLDDELGQILRKHTRIEMRSHLFWTLLHSSLPSRLPLPSNSGDDSSQLYSDIYGIVPSDVV

Target ORF information:

RefSeq Version	NM_001025812.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TIR domain-containing protein (tol-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025812.4

ORF Insert Sequence:

ATGAGGAGAA AAATGAAGTT ATTCCTATTT TTATTATTAG TAATTAATAT ATGTCGGTCG 
GCCGCTGCTA ACGGTGACGA ATGCCCGAAA TTCTGTAAAT GTGCTCCGGA TCCGGTGCAG 
CCGACGTCTA AACTCCTATT ATGCGACTAT TCTTCGAAAA ACACGACAAT TACACCTATT 
GCGTCGTCGA ATTATGATCA GGTTGCTAAT ATTCGATCAC TATTCATATC TTGTGATAAT 
AATAATTTCC AATTTCCGGA TGCCTACTTC AAGTCGTTAA CCGCGTTGCA TCATCTGCGG 
ATAGTGGGAT GCGAGACCAC ACATTTCTCT GTGAAATTGT TCGAAGATTT GGCCGCTTTG 
AGAAGATTGG AACTCGATCA GATCTCCACC GCCTCAACCT CTTTCGAAAT GACCGAAGAC 
GTCCTAATGC CGTTGGCTCG TCTCGAAAAG TTTTCCCTCA CGAGATCACG GAATATCGAG 
CTTCCACAGC GACTTTTGTG CTCTCTGCCG CATTTACAGG TTCTGAATAT CTCGTCAAAT 
GAGCTTCCAT CACTGCGAAG AGAGGAATCG TGTGTCGCTC AGCAGCTTCT GATCGTCGAT 
TTGTCTAGAA ATCGGCTCAC CAACATCGAG CAATTCCTAC GTGGCATCCC GGCAATCCGG 
CAAATTTCGG TGGCTTACAA CTCGATCGCC GAGCTCGATT TATCGCTGGC AACTCCATTT 
CTACAACAAC TCGATGCTGA AGCCAATCGA ATCGTCGACT TGACGTCACT TCCAGGCACT 
GTTGTACACG TGAATTTGGC TGGAAACGCG CTGAAAAGGG TGCCGGATGC GGTAGCCGAG 
TTGGCGAGTC TTGTGGCGTT AAATGTGTCG AGAAATGAGA TTGAAGCCGG AAATTCGTCG 
GTTTTTTCTT CCCCAGAACT CGAAATGCTC GACGCCTCTT ACAACAAATT GGACAGTCTG 
CCCGTCGAAT GGCTTCAAAA ATGCGAAAAA CGCATTGCTC ACCTCCATTT GGAGCACAAT 
TCGATTGAGC AGTTGACTGG TGGAGTGCTG GCGAATGCGA CTAATTTACA GACGTTGGAC 
CTTTCATCGA ATCAACTTCG AGTTTTTCGA GACGAAGTAC TTCCGGAGAA TTCGAAAATT 
GGAAATTTGA GACTTTCCAA CAACTCTTTG GAGCTCCTCG AGCCGTCGAG CCTGAGCGGC 
TTGAAATTGG AATCCCTTGA TCTGAGTCAT AATAAGCTGA CAGAAGTGCC CGCCGCAATT 
GGAAAAGTCG AGCAGCTGAA AAAAGTGGAT TTGAGCCATA ATAGAATTGC GAAGGTTTAT 
CAATATGTGC TCAATAAGAT TAAGCAATTG CATACTGTTG ATTTGTCGAA TAATCAGTTG 
CAAAGTATCG GCCCCTACAT CTTCTCCGAC AGTTCTGAAC TTCATTCCCT GGACGTGTCG 
AATAATGAGA TTTCACTGCT GTTCAAGGAC GCTTTTGCGA GATGCCCAAA GCTGAGGAAA 
ATTTCGATGA AAATGAATAA AATTAAATCC CTCGACGAAG GTCTCACAGA AGCTTCCGGC 
CTCCGACGTC TCGACGTATC TCATAACGAG ATCCTCGTGC TGAAATGGTC GGCTTTACCT 
GAAAACTTGG AGATTCTCAA CGCTGATAAC AATGATATCA ATCTCCTGAC CGCCGCCTCA 
ATGTCCCCAA GCACCGCAAA CTTGAAGTCC GTTTCGCTTT CCAACAACGG CATCACCATA 
ATGAATGCGG ACCAGATTCC GAATTCGCTC GAGTCGCTGG ACGTGTCGAA TAATCGACTT 
GCAAAGCTCG GGAAGACAGC GTTGGCCGCG AAATCTCAGT TGAGAAGGCT CAACTTGAAG 
GGCAATCTGC TTACCGTAGT GGCCACCGAG TCGATGAAAG TCGTAGAGGC TGTGCATCCG 
TTGAAAGTGG AAATCTCGGA GAATCCTCTG ATCTGTGATT GTCAGATGGG ATGGATGATT 
GGTGGAGCGA AGCCAAAGGT TCTCATTCAG GACTCTGAAA CCGCAAGCTG TTCCCATGCC 
GTTGATGGGC ATCAGATCCA GATTCAAAGT CTCAGCAAGA AGGATCTACT GTGCCCATAC 
AAAAGTGTAT GTGAGCCGGA ATGTATCTGC TGTCAATACG GAAATTGCGA TTGCAAATCC 
GTATGCCCCG CCAATTGCCG ATGCTTCAGA GATGATCAGT TTAATATCAA CATTGTCAGA 
TGCCACGGGA ACTCATCAAT GGTGCCCAAA AGAGAATTCG TGGTCTCCGA GCTCCCGGTC 
TCTGCGACAG AGATCATTCT GAGCGGAGTC ACCCTTCCAC AGCTCCGAAC TCACAGCTTC 
ATCGGAAGAC TTCGTCTCCA GAGGCTTCAT ATCAATGGAA CCGGGCTCCG ATCCATCCAA 
CCGAAGGCTT TCCATACTCT TCCAGCACTG AAGACGCTGG ATTTGTCGGA TAACTCGTTG 
ATCTCGCTGA GCGGGGAGGA ATTTCTAAAG TGTGGAGAAG TCTCGCAGCT TTTCCTCAAT 
GGAAATCGAT TTTCCACGCT ATCCCGTGGA ATCTTCGAGA AGCTTCCGAA CTTGAAATAT 
CTGACACTTC ATAACAACTC CCTCGAAGAC ATCCCTCAGG TTCTTCACTC GACGGCGCTC 
TCCAAGATCT CCCTGTCATC GAACCCCTTG AGATGCGACT GCTCGGGAGG ATCCCAACAG 
CACCTTCACC ATCGTCGTGA CCCAAAAGCT CATCCATTCT GGGAGCATAA TGCGGCCGAG 
TGGTTCTCGT TGCATCGGCA TCTTGTCGTT GATTTCCCCA AGGTTGAATG CTGGGAGAAC 
GTGACGAAGG CCTTCCTGAC GAACGATACG ACAGTGCTGA GCGCCTATCC ACCTAATATG 
GGAAATGACG TCTTTGTGAT GCCTATTGAA GAATTCCTGC GCGACTACAA CTCAACAATC 
TGTGTTCCAT TCTCATCTGG ATTCTTTGGA CAAGACCCTC AGAATAGTAT ACTCTTTGTA 
ATAATAACTA TATCGATTGC TGTTCTCCTC TGTGTCCTCG TTATTCTCGC AATTTCATTT 
ATTCGAAAAT CTCACGACGC AATCAATCAA CGAAGATACA AAGCATCATC TCTAAATTGT 
TCAACATCAG CCGGCTCGTC GCCTCTTCCG GTTCCGCTGT TGAGTTATCA CGCATTTGTG 
AGCTATTCGA AGAAGGACGA GAAAATGGTG ATTGATCAAT TGTGTCGACC GCTGGAAGAT 
GAAGATTATC AGTTGTGCCT GTTGCATCGG GATGGGCCGA CTTATTGCTC GAATTTGCAC 
GCGATATCTG ATGAACTCAT AGCTCAGATG GACTCGTCGC AATGCTTGAT TCTTGTGTTG 
ACTAAACACT TTTTGGAGAA CGAGTGGAAA ACGCTGCAGA TTAAGACCTC CCACCAACTA 
TTCGCCAAAA ACCGTGCAAA ACGAGTGATC GCCGTGCTCG GCGACGGTGT GGACGCGAAT 
CTGCTGGACG ATGAGCTCGG ACAGATTCTA CGGAAGCACA CGAGAATCGA GATGCGGAGC 
CATTTATTCT GGACACTTTT GCACTCATCA CTTCCATCAC GACTTCCATT ACCATCGAAT 
AGTGGCGATG ATTCGTCTCA ACTATATTCG GATATCTATG GAATTGTGCC TTCCGATGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tol-1 ( NM_001025812.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001020983.1 Caenorhabditis elegans TOLl (Drosophila) family (tol-1), partial mRNA.

Price & Availability↑

CloneID	OCa336436
Clone ID Related Accession (Same CDS sequence)	NM_001025812.4 , NM_001025812.3
Accession Version	NM_001025812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	TOLl (Drosophila) family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_001025812.2. COMPLETENESS: incomplete on both ends.

ATGAGGAGAA AAATGAAGTT ATTCCTATTT TTATTATTAG TAATTAATAT ATGTCGGTCG 
GCCGCTGCTA ACGGTGACGA ATGCCCGAAA TTCTGTAAAT GTGCTCCGGA TCCGGTGCAG 
CCGACGTCTA AACTCCTATT ATGCGACTAT TCTTCGAAAA ACACGACAAT TACACCTATT 
GCGTCGTCGA ATTATGATCA GGTTGCTAAT ATTCGATCAC TATTCATATC TTGTGATAAT 
AATAATTTCC AATTTCCGGA TGCCTACTTC AAGTCGTTAA CCGCGTTGCA TCATCTGCGG 
ATAGTGGGAT GCGAGACCAC ACATTTCTCT GTGAAATTGT TCGAAGATTT GGCCGCTTTG 
AGAAGATTGG AACTCGATCA GATCTCCACC GCCTCAACCT CTTTCGAAAT GACCGAAGAC 
GTCCTAATGC CGTTGGCTCG TCTCGAAAAG TTTTCCCTCA CGAGATCACG GAATATCGAG 
CTTCCACAGC GACTTTTGTG CTCTCTGCCG CATTTACAGG TTCTGAATAT CTCGTCAAAT 
GAGCTTCCAT CACTGCGAAG AGAGGAATCG TGTGTCGCTC AGCAGCTTCT GATCGTCGAT 
TTGTCTAGAA ATCGGCTCAC CAACATCGAG CAATTCCTAC GTGGCATCCC GGCAATCCGG 
CAAATTTCGG TGGCTTACAA CTCGATCGCC GAGCTCGATT TATCGCTGGC AACTCCATTT 
CTACAACAAC TCGATGCTGA AGCCAATCGA ATCGTCGACT TGACGTCACT TCCAGGCACT 
GTTGTACACG TGAATTTGGC TGGAAACGCG CTGAAAAGGG TGCCGGATGC GGTAGCCGAG 
TTGGCGAGTC TTGTGGCGTT AAATGTGTCG AGAAATGAGA TTGAAGCCGG AAATTCGTCG 
GTTTTTTCTT CCCCAGAACT CGAAATGCTC GACGCCTCTT ACAACAAATT GGACAGTCTG 
CCCGTCGAAT GGCTTCAAAA ATGCGAAAAA CGCATTGCTC ACCTCCATTT GGAGCACAAT 
TCGATTGAGC AGTTGACTGG TGGAGTGCTG GCGAATGCGA CTAATTTACA GACGTTGGAC 
CTTTCATCGA ATCAACTTCG AGTTTTTCGA GACGAAGTAC TTCCGGAGAA TTCGAAAATT 
GGAAATTTGA GACTTTCCAA CAACTCTTTG GAGCTCCTCG AGCCGTCGAG CCTGAGCGGC 
TTGAAATTGG AATCCCTTGA TCTGAGTCAT AATAAGCTGA CAGAAGTGCC CGCCGCAATT 
GGAAAAGTCG AGCAGCTGAA AAAAGTGGAT TTGAGCCATA ATAGAATTGC GAAGGTTTAT 
CAATATGTGC TCAATAAGAT TAAGCAATTG CATACTGTTG ATTTGTCGAA TAATCAGTTG 
CAAAGTATCG GCCCCTACAT CTTCTCCGAC AGTTCTGAAC TTCATTCCCT GGACGTGTCG 
AATAATGAGA TTTCACTGCT GTTCAAGGAC GCTTTTGCGA GATGCCCAAA GCTGAGGAAA 
ATTTCGATGA AAATGAATAA AATTAAATCC CTCGACGAAG GTCTCACAGA AGCTTCCGGC 
CTCCGACGTC TCGACGTATC TCATAACGAG ATCCTCGTGC TGAAATGGTC GGCTTTACCT 
GAAAACTTGG AGATTCTCAA CGCTGATAAC AATGATATCA ATCTCCTGAC CGCCGCCTCA 
ATGTCCCCAA GCACCGCAAA CTTGAAGTCC GTTTCGCTTT CCAACAACGG CATCACCATA 
ATGAATGCGG ACCAGATTCC GAATTCGCTC GAGTCGCTGG ACGTGTCGAA TAATCGACTT 
GCAAAGCTCG GGAAGACAGC GTTGGCCGCG AAATCTCAGT TGAGAAGGCT CAACTTGAAG 
GGCAATCTGC TTACCGTAGT GGCCACCGAG TCGATGAAAG TCGTAGAGGC TGTGCATCCG 
TTGAAAGTGG AAATCTCGGA GAATCCTCTG ATCTGTGATT GTCAGATGGG ATGGATGATT 
GGTGGAGCGA AGCCAAAGGT TCTCATTCAG GACTCTGAAA CCGCAAGCTG TTCCCATGCC 
GTTGATGGGC ATCAGATCCA GATTCAAAGT CTCAGCAAGA AGGATCTACT GTGCCCATAC 
AAAAGTGTAT GTGAGCCGGA ATGTATCTGC TGTCAATACG GAAATTGCGA TTGCAAATCC 
GTATGCCCCG CCAATTGCCG ATGCTTCAGA GATGATCAGT TTAATATCAA CATTGTCAGA 
TGCCACGGGA ACTCATCAAT GGTGCCCAAA AGAGAATTCG TGGTCTCCGA GCTCCCGGTC 
TCTGCGACAG AGATCATTCT GAGCGGAGTC ACCCTTCCAC AGCTCCGAAC TCACAGCTTC 
ATCGGAAGAC TTCGTCTCCA GAGGCTTCAT ATCAATGGAA CCGGGCTCCG ATCCATCCAA 
CCGAAGGCTT TCCATACTCT TCCAGCACTG AAGACGCTGG ATTTGTCGGA TAACTCGTTG 
ATCTCGCTGA GCGGGGAGGA ATTTCTAAAG TGTGGAGAAG TCTCGCAGCT TTTCCTCAAT 
GGAAATCGAT TTTCCACGCT ATCCCGTGGA ATCTTCGAGA AGCTTCCGAA CTTGAAATAT 
CTGACACTTC ATAACAACTC CCTCGAAGAC ATCCCTCAGG TTCTTCACTC GACGGCGCTC 
TCCAAGATCT CCCTGTCATC GAACCCCTTG AGATGCGACT GCTCGGGAGG ATCCCAACAG 
CACCTTCACC ATCGTCGTGA CCCAAAAGCT CATCCATTCT GGGAGCATAA TGCGGCCGAG 
TGGTTCTCGT TGCATCGGCA TCTTGTCGTT GATTTCCCCA AGGTTGAATG CTGGGAGAAC 
GTGACGAAGG CCTTCCTGAC GAACGATACG ACAGTGCTGA GCGCCTATCC ACCTAATATG 
GGAAATGACG TCTTTGTGAT GCCTATTGAA GAATTCCTGC GCGACTACAA CTCAACAATC 
TGTGTTCCAT TCTCATCTGG ATTCTTTGGA CAAGACCCTC AGAATAGTAT ACTCTTTGTA 
ATAATAACTA TATCGATTGC TGTTCTCCTC TGTGTCCTCG TTATTCTCGC AATTTCATTT 
ATTCGAAAAT CTCACGACGC AATCAATCAA CGAAGATACA AAGCATCATC TCTAAATTGT 
TCAACATCAG CCGGCTCGTC GCCTCTTCCG GTTCCGCTGT TGAGTTATCA CGCATTTGTG 
AGCTATTCGA AGAAGGACGA GAAAATGGTG ATTGATCAAT TGTGTCGACC GCTGGAAGAT 
GAAGATTATC AGTTGTGCCT GTTGCATCGG GATGGGCCGA CTTATTGCTC GAATTTGCAC 
GCGATATCTG ATGAACTCAT AGCTCAGATG GACTCGTCGC AATGCTTGAT TCTTGTGTTG 
ACTAAACACT TTTTGGAGAA CGAGTGGAAA ACGCTGCAGA TTAAGACCTC CCACCAACTA 
TTCGCCAAAA ACCGTGCAAA ACGAGTGATC GCCGTGCTCG GCGACGGTGT GGACGCGAAT 
CTGCTGGACG ATGAGCTCGG ACAGATTCTA CGGAAGCACA CGAGAATCGA GATGCGGAGC 
CATTTATTCT GGACACTTTT GCACTCATCA CTTCCATCAC GACTTCCATT ACCATCGAAT 
AGTGGCGATG ATTCGTCTCA ACTATATTCG GATATCTATG GAATTGTGCC TTCCGATGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020983.1
CDS	1..3666
Translation	MRRKMKLFLFLLLVINICRSAAANGDECPKFCKCAPDPVQPTSKLLLCDYSSKNTTITPIASSNYDQVANIRSLFISCDNNNFQFPDAYFKSLTALHHLRIVGCETTHFSVKLFEDLAALRRLELDQISTASTSFEMTEDVLMPLARLEKFSLTRSRNIELPQRLLCSLPHLQVLNISSNELPSLRREESCVAQQLLIVDLSRNRLTNIEQFLRGIPAIRQISVAYNSIAELDLSLATPFLQQLDAEANRIVDLTSLPGTVVHVNLAGNALKRVPDAVAELASLVALNVSRNEIEAGNSSVFSSPELEMLDASYNKLDSLPVEWLQKCEKRIAHLHLEHNSIEQLTGGVLANATNLQTLDLSSNQLRVFRDEVLPENSKIGNLRLSNNSLELLEPSSLSGLKLESLDLSHNKLTEVPAAIGKVEQLKKVDLSHNRIAKVYQYVLNKIKQLHTVDLSNNQLQSIGPYIFSDSSELHSLDVSNNEISLLFKDAFARCPKLRKISMKMNKIKSLDEGLTEASGLRRLDVSHNEILVLKWSALPENLEILNADNNDINLLTAASMSPSTANLKSVSLSNNGITIMNADQIPNSLESLDVSNNRLAKLGKTALAAKSQLRRLNLKGNLLTVVATESMKVVEAVHPLKVEISENPLICDCQMGWMIGGAKPKVLIQDSETASCSHAVDGHQIQIQSLSKKDLLCPYKSVCEPECICCQYGNCDCKSVCPANCRCFRDDQFNINIVRCHGNSSMVPKREFVVSELPVSATEIILSGVTLPQLRTHSFIGRLRLQRLHINGTGLRSIQPKAFHTLPALKTLDLSDNSLISLSGEEFLKCGEVSQLFLNGNRFSTLSRGIFEKLPNLKYLTLHNNSLEDIPQVLHSTALSKISLSSNPLRCDCSGGSQQHLHHRRDPKAHPFWEHNAAEWFSLHRHLVVDFPKVECWENVTKAFLTNDTTVLSAYPPNMGNDVFVMPIEEFLRDYNSTICVPFSSGFFGQDPQNSILFVIITISIAVLLCVLVILAISFIRKSHDAINQRRYKASSLNCSTSAGSSPLPVPLLSYHAFVSYSKKDEKMVIDQLCRPLEDEDYQLCLLHRDGPTYCSNLHAISDELIAQMDSSQCLILVLTKHFLENEWKTLQIKTSHQLFAKNRAKRVIAVLGDGVDANLLDDELGQILRKHTRIEMRSHLFWTLLHSSLPSRLPLPSNSGDDSSQLYSDIYGIVPSDVV

Target ORF information:

RefSeq Version	NM_001025812.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans TOLl (Drosophila) family (tol-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025812.3

ORF Insert Sequence:

ATGAGGAGAA AAATGAAGTT ATTCCTATTT TTATTATTAG TAATTAATAT ATGTCGGTCG 
GCCGCTGCTA ACGGTGACGA ATGCCCGAAA TTCTGTAAAT GTGCTCCGGA TCCGGTGCAG 
CCGACGTCTA AACTCCTATT ATGCGACTAT TCTTCGAAAA ACACGACAAT TACACCTATT 
GCGTCGTCGA ATTATGATCA GGTTGCTAAT ATTCGATCAC TATTCATATC TTGTGATAAT 
AATAATTTCC AATTTCCGGA TGCCTACTTC AAGTCGTTAA CCGCGTTGCA TCATCTGCGG 
ATAGTGGGAT GCGAGACCAC ACATTTCTCT GTGAAATTGT TCGAAGATTT GGCCGCTTTG 
AGAAGATTGG AACTCGATCA GATCTCCACC GCCTCAACCT CTTTCGAAAT GACCGAAGAC 
GTCCTAATGC CGTTGGCTCG TCTCGAAAAG TTTTCCCTCA CGAGATCACG GAATATCGAG 
CTTCCACAGC GACTTTTGTG CTCTCTGCCG CATTTACAGG TTCTGAATAT CTCGTCAAAT 
GAGCTTCCAT CACTGCGAAG AGAGGAATCG TGTGTCGCTC AGCAGCTTCT GATCGTCGAT 
TTGTCTAGAA ATCGGCTCAC CAACATCGAG CAATTCCTAC GTGGCATCCC GGCAATCCGG 
CAAATTTCGG TGGCTTACAA CTCGATCGCC GAGCTCGATT TATCGCTGGC AACTCCATTT 
CTACAACAAC TCGATGCTGA AGCCAATCGA ATCGTCGACT TGACGTCACT TCCAGGCACT 
GTTGTACACG TGAATTTGGC TGGAAACGCG CTGAAAAGGG TGCCGGATGC GGTAGCCGAG 
TTGGCGAGTC TTGTGGCGTT AAATGTGTCG AGAAATGAGA TTGAAGCCGG AAATTCGTCG 
GTTTTTTCTT CCCCAGAACT CGAAATGCTC GACGCCTCTT ACAACAAATT GGACAGTCTG 
CCCGTCGAAT GGCTTCAAAA ATGCGAAAAA CGCATTGCTC ACCTCCATTT GGAGCACAAT 
TCGATTGAGC AGTTGACTGG TGGAGTGCTG GCGAATGCGA CTAATTTACA GACGTTGGAC 
CTTTCATCGA ATCAACTTCG AGTTTTTCGA GACGAAGTAC TTCCGGAGAA TTCGAAAATT 
GGAAATTTGA GACTTTCCAA CAACTCTTTG GAGCTCCTCG AGCCGTCGAG CCTGAGCGGC 
TTGAAATTGG AATCCCTTGA TCTGAGTCAT AATAAGCTGA CAGAAGTGCC CGCCGCAATT 
GGAAAAGTCG AGCAGCTGAA AAAAGTGGAT TTGAGCCATA ATAGAATTGC GAAGGTTTAT 
CAATATGTGC TCAATAAGAT TAAGCAATTG CATACTGTTG ATTTGTCGAA TAATCAGTTG 
CAAAGTATCG GCCCCTACAT CTTCTCCGAC AGTTCTGAAC TTCATTCCCT GGACGTGTCG 
AATAATGAGA TTTCACTGCT GTTCAAGGAC GCTTTTGCGA GATGCCCAAA GCTGAGGAAA 
ATTTCGATGA AAATGAATAA AATTAAATCC CTCGACGAAG GTCTCACAGA AGCTTCCGGC 
CTCCGACGTC TCGACGTATC TCATAACGAG ATCCTCGTGC TGAAATGGTC GGCTTTACCT 
GAAAACTTGG AGATTCTCAA CGCTGATAAC AATGATATCA ATCTCCTGAC CGCCGCCTCA 
ATGTCCCCAA GCACCGCAAA CTTGAAGTCC GTTTCGCTTT CCAACAACGG CATCACCATA 
ATGAATGCGG ACCAGATTCC GAATTCGCTC GAGTCGCTGG ACGTGTCGAA TAATCGACTT 
GCAAAGCTCG GGAAGACAGC GTTGGCCGCG AAATCTCAGT TGAGAAGGCT CAACTTGAAG 
GGCAATCTGC TTACCGTAGT GGCCACCGAG TCGATGAAAG TCGTAGAGGC TGTGCATCCG 
TTGAAAGTGG AAATCTCGGA GAATCCTCTG ATCTGTGATT GTCAGATGGG ATGGATGATT 
GGTGGAGCGA AGCCAAAGGT TCTCATTCAG GACTCTGAAA CCGCAAGCTG TTCCCATGCC 
GTTGATGGGC ATCAGATCCA GATTCAAAGT CTCAGCAAGA AGGATCTACT GTGCCCATAC 
AAAAGTGTAT GTGAGCCGGA ATGTATCTGC TGTCAATACG GAAATTGCGA TTGCAAATCC 
GTATGCCCCG CCAATTGCCG ATGCTTCAGA GATGATCAGT TTAATATCAA CATTGTCAGA 
TGCCACGGGA ACTCATCAAT GGTGCCCAAA AGAGAATTCG TGGTCTCCGA GCTCCCGGTC 
TCTGCGACAG AGATCATTCT GAGCGGAGTC ACCCTTCCAC AGCTCCGAAC TCACAGCTTC 
ATCGGAAGAC TTCGTCTCCA GAGGCTTCAT ATCAATGGAA CCGGGCTCCG ATCCATCCAA 
CCGAAGGCTT TCCATACTCT TCCAGCACTG AAGACGCTGG ATTTGTCGGA TAACTCGTTG 
ATCTCGCTGA GCGGGGAGGA ATTTCTAAAG TGTGGAGAAG TCTCGCAGCT TTTCCTCAAT 
GGAAATCGAT TTTCCACGCT ATCCCGTGGA ATCTTCGAGA AGCTTCCGAA CTTGAAATAT 
CTGACACTTC ATAACAACTC CCTCGAAGAC ATCCCTCAGG TTCTTCACTC GACGGCGCTC 
TCCAAGATCT CCCTGTCATC GAACCCCTTG AGATGCGACT GCTCGGGAGG ATCCCAACAG 
CACCTTCACC ATCGTCGTGA CCCAAAAGCT CATCCATTCT GGGAGCATAA TGCGGCCGAG 
TGGTTCTCGT TGCATCGGCA TCTTGTCGTT GATTTCCCCA AGGTTGAATG CTGGGAGAAC 
GTGACGAAGG CCTTCCTGAC GAACGATACG ACAGTGCTGA GCGCCTATCC ACCTAATATG 
GGAAATGACG TCTTTGTGAT GCCTATTGAA GAATTCCTGC GCGACTACAA CTCAACAATC 
TGTGTTCCAT TCTCATCTGG ATTCTTTGGA CAAGACCCTC AGAATAGTAT ACTCTTTGTA 
ATAATAACTA TATCGATTGC TGTTCTCCTC TGTGTCCTCG TTATTCTCGC AATTTCATTT 
ATTCGAAAAT CTCACGACGC AATCAATCAA CGAAGATACA AAGCATCATC TCTAAATTGT 
TCAACATCAG CCGGCTCGTC GCCTCTTCCG GTTCCGCTGT TGAGTTATCA CGCATTTGTG 
AGCTATTCGA AGAAGGACGA GAAAATGGTG ATTGATCAAT TGTGTCGACC GCTGGAAGAT 
GAAGATTATC AGTTGTGCCT GTTGCATCGG GATGGGCCGA CTTATTGCTC GAATTTGCAC 
GCGATATCTG ATGAACTCAT AGCTCAGATG GACTCGTCGC AATGCTTGAT TCTTGTGTTG 
ACTAAACACT TTTTGGAGAA CGAGTGGAAA ACGCTGCAGA TTAAGACCTC CCACCAACTA 
TTCGCCAAAA ACCGTGCAAA ACGAGTGATC GCCGTGCTCG GCGACGGTGT GGACGCGAAT 
CTGCTGGACG ATGAGCTCGG ACAGATTCTA CGGAAGCACA CGAGAATCGA GATGCGGAGC 
CATTTATTCT GGACACTTTT GCACTCATCA CTTCCATCAC GACTTCCATT ACCATCGAAT 
AGTGGCGATG ATTCGTCTCA ACTATATTCG GATATCTATG GAATTGTGCC TTCCGATGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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tol-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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