GIT1 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following GIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu62253 XM_025280963.1
Latest version!
Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu62254 XM_025280964.1
Latest version!
Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu62255 XM_025280965.1
Latest version!
Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu01796 XM_006042215.1
Latest version!
Bubalus bubalis G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu62253
    Clone ID Related Accession (Same CDS sequence) XM_025280963.1
    Accession Version XM_025280963.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product ARF GTPase-activating protein GIT1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GCCTGGACCT GTCCGAGTTG
    GCCAAAGCTG CCAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTAGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGG GAAAATGACG CTGTGTGGCT GGCGACCCAG
    AACCACAGCA CCCTGGTGAC CGAGCGCAGC GCCGTGCCCT TCCTGCCAGT GAACCCCGAG
    TACTCAGCCA CACGGAATCA GGGGCGACAG AAGCTGGCCC GCTTTAACGC CCGGGAGTTC
    GCCACCTTGA TCATCGACAT CCTCAGCGAG GCCAAGAGGA GACAGCAGGG CAAGAGCCTG
    GGCAGCCCCA CAGACAACCT CGAGCTGTCC GCGCGGGGCC AGAGTGACCT GGACGACCAG
    CACGACTACG ACAGCGTGGC CTCGGACGAG GACACAGACC AGGAGCCCCT GCGCAGTGCT
    GGTGCCACGC GGAACAACCG AGCTCGGAGC ATGGACTCCT CGGACCTGTC AGACGGGGCT
    GTGACGCTGC AAGAGTACCT GGAGCTGAAA AAGGCCCTGG CCGCTTCCGA GGCCAAGGTG
    CAGCAGCTCA TGAAGGTCAA TAGCAGCCTG AGCGATGAGC TCCGGAGGCT GCAGAGGGAG
    ATCCACAAGC TGCAGGCCGA GAACCTGCAG ATCCGGCAGC CACCAGGGCC GGTGCCCACA
    CCCCCCCTCC CCAGCGAGCG AGCTGAGCAC GCACCCGTGG GGCCTGGCGG GAGCACCCAC
    CGCAGGGACC GCCAGGCCTT TTCCATGTAT GAACCAGGCT CCGCCCTGAA GCCCTTTGGG
    GTCCCGCCCG GGGACGAGCT CACCACCCGG CTCCAGCCTT TCCACAGCAC CGAGCTGGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCCGGCCTTT ACCGGATCCG GAAGGGGGTG
    TCGGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCACTGC TGTCGTGCTC CCAGGAAGGA
    AGCCGCCACA CGAGCAAGCT CTCCCGCCAC GGCAGCGGCG CAGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCGCT GCTGGGCCTG GAAGGGAAGA GGTTTCTGGA GCTGGGCAAG
    GAAGAGGACT TCCACCCGGA GTTGGAAAGC CTGGATGGCG ACCTCGACCC TGGGCTTCCC
    AGCACGGAGG ATGTCATCCT GAAGACGGAG CAGGTCACCA AGAACATTCA GGAGTTACTG
    CGGGCGGCCC AGGAATTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCCGTGACTG AGATGGCCTC TCTCTTCCCG AAGAGGCCCG CCCTGGAGCC CGTGCGAAGC
    TCGCTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GCGAGTGCCG GAAGACGGTG
    CCCCCAGAGC CCGGCGCTCC CGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136748.1
    CDS107..2392
    Translation

    Target ORF information:

    RefSeq Version XM_025280963.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280963.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GCCTGGACCT GTCCGAGTTG
    GCCAAAGCTG CCAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTAGCC
    ATGGACGTGT ATGACGAGGT GGATCGAAGG GAAAATGACG CTGTGTGGCT GGCGACCCAG
    AACCACAGCA CCCTGGTGAC CGAGCGCAGC GCCGTGCCCT TCCTGCCAGT GAACCCCGAG
    TACTCAGCCA CACGGAATCA GGGGCGACAG AAGCTGGCCC GCTTTAACGC CCGGGAGTTC
    GCCACCTTGA TCATCGACAT CCTCAGCGAG GCCAAGAGGA GACAGCAGGG CAAGAGCCTG
    GGCAGCCCCA CAGACAACCT CGAGCTGTCC GCGCGGGGCC AGAGTGACCT GGACGACCAG
    CACGACTACG ACAGCGTGGC CTCGGACGAG GACACAGACC AGGAGCCCCT GCGCAGTGCT
    GGTGCCACGC GGAACAACCG AGCTCGGAGC ATGGACTCCT CGGACCTGTC AGACGGGGCT
    GTGACGCTGC AAGAGTACCT GGAGCTGAAA AAGGCCCTGG CCGCTTCCGA GGCCAAGGTG
    CAGCAGCTCA TGAAGGTCAA TAGCAGCCTG AGCGATGAGC TCCGGAGGCT GCAGAGGGAG
    ATCCACAAGC TGCAGGCCGA GAACCTGCAG ATCCGGCAGC CACCAGGGCC GGTGCCCACA
    CCCCCCCTCC CCAGCGAGCG AGCTGAGCAC GCACCCGTGG GGCCTGGCGG GAGCACCCAC
    CGCAGGGACC GCCAGGCCTT TTCCATGTAT GAACCAGGCT CCGCCCTGAA GCCCTTTGGG
    GTCCCGCCCG GGGACGAGCT CACCACCCGG CTCCAGCCTT TCCACAGCAC CGAGCTGGAG
    GACGACGCCA TCTATTCAGT GCACGTCCCT GCCGGCCTTT ACCGGATCCG GAAGGGGGTG
    TCGGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCACTGC TGTCGTGCTC CCAGGAAGGA
    AGCCGCCACA CGAGCAAGCT CTCCCGCCAC GGCAGCGGCG CAGACAGTGA CTATGAGAAC
    ACGCAAAGTG GGGACCCGCT GCTGGGCCTG GAAGGGAAGA GGTTTCTGGA GCTGGGCAAG
    GAAGAGGACT TCCACCCGGA GTTGGAAAGC CTGGATGGCG ACCTCGACCC TGGGCTTCCC
    AGCACGGAGG ATGTCATCCT GAAGACGGAG CAGGTCACCA AGAACATTCA GGAGTTACTG
    CGGGCGGCCC AGGAATTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG
    GCCGTGACTG AGATGGCCTC TCTCTTCCCG AAGAGGCCCG CCCTGGAGCC CGTGCGAAGC
    TCGCTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GCGAGTGCCG GAAGACGGTG
    CCCCCAGAGC CCGGCGCTCC CGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC
    GCCTATGACA TCGCCAAGGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAGAAGAAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62254
    Clone ID Related Accession (Same CDS sequence) XM_025280964.1
    Accession Version XM_025280964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product ARF GTPase-activating protein GIT1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA
    AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC
    GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG
    CTGGCCCGCT TTAACGCCCG GGAGTTCGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC
    AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCGCG
    CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACCGAGC TCGGAGCATG
    GACTCCTCGG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG
    GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGC
    GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC
    CGGCAGCCAC CAGGGCCGGT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA
    CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA
    CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC
    CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCC
    GGCCTTTACC GGGAAGGAAG CCGCCACACG AGCAAGCTCT CCCGCCACGG CAGCGGCGCA
    GACAGTGACT ATGAGAACAC GCAAAGTGGG GACCCGCTGC TGGGCCTGGA AGGGAAGAGG
    TTTCTGGAGC TGGGCAAGGA AGAGGACTTC CACCCGGAGT TGGAAAGCCT GGATGGCGAC
    CTCGACCCTG GGCTTCCCAG CACGGAGGAT GTCATCCTGA AGACGGAGCA GGTCACCAAG
    AACATTCAGG AGTTACTGCG GGCGGCCCAG GAATTCAAGC ATGACAGCTT CGTGCCCTGC
    TCAGAGAAGA TCCATTTGGC CGTGACTGAG ATGGCCTCTC TCTTCCCGAA GAGGCCCGCC
    CTGGAGCCCG TGCGAAGCTC GCTGCGGCTG CTCAACGCCA GCGCCTACCG GCTGCAGAGC
    GAGTGCCGGA AGACGGTGCC CCCAGAGCCC GGCGCTCCCG TGGACTTCCA GCTGCTGACT
    CAGCAGGTGA TCCAGTGCGC CTATGACATC GCCAAGGCCG CCAAGCAGCT GGTCACCATC
    ACCACCCGCG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136749.1
    CDS110..2353
    Translation

    Target ORF information:

    RefSeq Version XM_025280964.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA
    AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC
    GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG
    CTGGCCCGCT TTAACGCCCG GGAGTTCGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC
    AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCGCG
    CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACCGAGC TCGGAGCATG
    GACTCCTCGG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG
    GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGC
    GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC
    CGGCAGCCAC CAGGGCCGGT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA
    CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA
    CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC
    CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCC
    GGCCTTTACC GGGAAGGAAG CCGCCACACG AGCAAGCTCT CCCGCCACGG CAGCGGCGCA
    GACAGTGACT ATGAGAACAC GCAAAGTGGG GACCCGCTGC TGGGCCTGGA AGGGAAGAGG
    TTTCTGGAGC TGGGCAAGGA AGAGGACTTC CACCCGGAGT TGGAAAGCCT GGATGGCGAC
    CTCGACCCTG GGCTTCCCAG CACGGAGGAT GTCATCCTGA AGACGGAGCA GGTCACCAAG
    AACATTCAGG AGTTACTGCG GGCGGCCCAG GAATTCAAGC ATGACAGCTT CGTGCCCTGC
    TCAGAGAAGA TCCATTTGGC CGTGACTGAG ATGGCCTCTC TCTTCCCGAA GAGGCCCGCC
    CTGGAGCCCG TGCGAAGCTC GCTGCGGCTG CTCAACGCCA GCGCCTACCG GCTGCAGAGC
    GAGTGCCGGA AGACGGTGCC CCCAGAGCCC GGCGCTCCCG TGGACTTCCA GCTGCTGACT
    CAGCAGGTGA TCCAGTGCGC CTATGACATC GCCAAGGCCG CCAAGCAGCT GGTCACCATC
    ACCACCCGCG AGAAGAAGCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu62255
    Clone ID Related Accession (Same CDS sequence) XM_025280965.1
    Accession Version XM_025280965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product ARF GTPase-activating protein GIT1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA
    AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC
    GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG
    CTGGCCCGCT TTAACGCCCG GGAGTTCGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC
    AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCGCG
    CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACCGAGC TCGGAGCATG
    GACTCCTCGG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG
    GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGC
    GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC
    CGGCAGCCAC CAGGGCCGGT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA
    CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA
    CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC
    CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCC
    GGCCTTTACC GGATCCGGAA GGGGGTGTCG GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCACTGCTGT CGTGCTCCCA GGAAGGAAGC CGCCACACGA GCAAGCTCTC CCGCCACGGC
    AGCGGCGCAG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA
    GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA GAGGACTTCC ACCCGGAGTT GGAAAGCCTG
    GATGGCGACC TCGACCCTGG GCTTCCCAGC ACGGAGGATG TCATCCTGAA GACGGAGCAG
    GTCACCAAGA ACATTCAGGA GTTACTGCGG GCGGCCCAGG AATTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCC GTGACTGAGA TGGCCTCTCT CTTCCCGAAG
    AGGCCCGCCC TGGAGCCCGT GCGAAGCTCG CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGCG AGTGCCGGAA GACGGTGCCC CCAGAGCCCG GCGCTCCCGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCCGC CAAGCAGCTG
    GTCACCATCA CCACCCGCGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025136750.1
    CDS114..2426
    Translation

    Target ORF information:

    RefSeq Version XM_025280965.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis GIT ArfGAP 1 (GIT1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025280965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCCCGGA AGGGGCCGCG AGCGGAGGTG TGTGCAGACT GCAGCGCCCC GGACCCCGGC 
    TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG TGTGACGAGT GCTGCAGCGT GCACCGGAGT
    CTGGGCCGCC ACATCTCCAT TGTCAAGCAC CTCCGCCACA GCGCCTGGCC CCCCACCCTG
    CTGCAGATGG TGCACACGCT CGCCAGCAAC GGGGCCAACT CCATCTGGGA GCACTCCCTG
    CTGGACCCCG CGCAGGTGCA GAGCGGCCGG CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC
    CCCATCAAGT CTGAGTTCAT CAGGGCCAAG TACCAGATGC TGGCTTTTGT GCACAAGCTG
    CCCTGCCGGG ACGATGACGG AGTCACCGCC AAAGACCTCA GCAAGCAACT GCACTCAAGC
    GTGCGAACGG GCAACCTGGA GACATGTCTG CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC
    TTCTTCCACC CCGAAAAGGG CACCACACCT CTGCATGTGG CTGCCAAGGC AGGTCAGACG
    CTGCAGGCCG AGCTGCTTGT GGTGTATGGG GCTGACCCTG GCTCCCCAGA TGTTAACGGC
    CGTACACCCA TTGATTATGC CAGGCAGGCG GGGCACCATG AGCTTGCGGA ACGGCTAGTC
    GAGTGCCAGT ACGAGCTCAC TGACCGGTTG GCCTTCTATC TCTGTGGGCG CAAGCCGGAT
    CACAAGAATG GGCATTACAT CATCCCACAG ATGGCAGACA GATCTCGGCA AAAGTGCATG
    TCTCAGAGCC TGGACCTGTC CGAGTTGGCC AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC
    AGCAACCGGC TTTTTGAGGA ACTAGCCATG GACGTGTATG ACGAGGTGGA TCGAAGGGAA
    AATGACGCTG TGTGGCTGGC GACCCAGAAC CACAGCACCC TGGTGACCGA GCGCAGCGCC
    GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC TCAGCCACAC GGAATCAGGG GCGACAGAAG
    CTGGCCCGCT TTAACGCCCG GGAGTTCGCC ACCTTGATCA TCGACATCCT CAGCGAGGCC
    AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC AGCCCCACAG ACAACCTCGA GCTGTCCGCG
    CGGGGCCAGA GTGACCTGGA CGACCAGCAC GACTACGACA GCGTGGCCTC GGACGAGGAC
    ACAGACCAGG AGCCCCTGCG CAGTGCTGGT GCCACGCGGA ACAACCGAGC TCGGAGCATG
    GACTCCTCGG ACCTGTCAGA CGGGGCTGTG ACGCTGCAAG AGTACCTGGA GCTGAAAAAG
    GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG CAGCTCATGA AGGTCAATAG CAGCCTGAGC
    GATGAGCTCC GGAGGCTGCA GAGGGAGATC CACAAGCTGC AGGCCGAGAA CCTGCAGATC
    CGGCAGCCAC CAGGGCCGGT GCCCACACCC CCCCTCCCCA GCGAGCGAGC TGAGCACGCA
    CCCGTGGGGC CTGGCGGGAG CACCCACCGC AGGGACCGCC AGGCCTTTTC CATGTATGAA
    CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC CCGCCCGGGG ACGAGCTCAC CACCCGGCTC
    CAGCCTTTCC ACAGCACCGA GCTGGAGGAC GACGCCATCT ATTCAGTGCA CGTCCCTGCC
    GGCCTTTACC GGATCCGGAA GGGGGTGTCG GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC
    CCACTGCTGT CGTGCTCCCA GGAAGGAAGC CGCCACACGA GCAAGCTCTC CCGCCACGGC
    AGCGGCGCAG ACAGTGACTA TGAGAACACG CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA
    GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA GAGGACTTCC ACCCGGAGTT GGAAAGCCTG
    GATGGCGACC TCGACCCTGG GCTTCCCAGC ACGGAGGATG TCATCCTGAA GACGGAGCAG
    GTCACCAAGA ACATTCAGGA GTTACTGCGG GCGGCCCAGG AATTCAAGCA TGACAGCTTC
    GTGCCCTGCT CAGAGAAGAT CCATTTGGCC GTGACTGAGA TGGCCTCTCT CTTCCCGAAG
    AGGCCCGCCC TGGAGCCCGT GCGAAGCTCG CTGCGGCTGC TCAACGCCAG CGCCTACCGG
    CTGCAGAGCG AGTGCCGGAA GACGGTGCCC CCAGAGCCCG GCGCTCCCGT GGACTTCCAG
    CTGCTGACTC AGCAGGTGAT CCAGTGCGCC TATGACATCG CCAAGGCCGC CAAGCAGCTG
    GTCACCATCA CCACCCGCGA GAAGAAGCAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu01796
    Clone ID Related Accession (Same CDS sequence) XM_006042215.1
    Accession Version XM_006042215.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2943bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product ARF GTPase-activating protein GIT1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005782421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCGGGGAT GCGGCGGAGA GAGGAGGGGG ACGCGGATGC CCTGGGCAGC GCTTGTTATC 
    ACTGGGCCCC GCGGCATCAT GGGGCTTGTA GTCCGCCTCG TGGAACCGGG GCTGGCCCCG
    GCGCGGAACG ATGAAGGAGG AGTGGACTGC CTCGCCCCTT TCTCCAGGCT GGGCGGAGCC
    AGTTGGCGTC TCCCGCTGCC GGGGACCGCT GCCCACCTCC ACCACGAGGG GTCACCAGAG
    TGCCGTGGCT GCAGCCGGAG TTGGGGAGGG GGCTGGAGCG GGGAAGGGAC GGCAGGAGTG
    GCCGGGAGAA TGGGTTACTG TCCCTGGGCC CGAGCTCCTC AGGCCACCCT CAGCCTCGAA
    TATGAAGGGG ACGAGGCTGT GAGGCGAACT CGCAGCGTCT CGGCGGCAGG GGCGCACCGA
    GGCCCGGGCC GCAGGCGGCG CTGCGCGCTC GGGCCTGGCG GCGGCGGCGG CCCCTTTAAA
    GTCCTGTGTG CTCCTGGGAA CCCCCTCCCT CCGCCCCCAC CTCGGGCCCC GATCCAGAAA
    GCAGGTCAGG AGGAGGCAGG TGCTGAGGGG ACTGAGTGTG CAAATTGTGC GTGGAGAGCT
    AAGCACAGTG CAGGTCCAGG GCCGTGCTCG GGAGGCTTAT CCGTCTGGCT GTGGCTGACC
    CTTCTCCCTG CTCACCCCGC AGACCCCGGC TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG
    TGTGACGAGT GCTGCAGCGT GCACCGGAGT CTGGGCCGCC ACATCTCCAT TGTCAAGCAC
    CTCCGCCACA GCGCCTGGCC CCCCACCCTG CTGCAGATGG TGCACACGCT CGCCAGCAAC
    GGGGCCAACT CCATCTGGGA GCACTCCCTG CTGGACCCCG CGCAGGTGCA GAGCGGCCGG
    CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC CCCATCAAGT CTGAGTTCAT CAGGGCCAAG
    TACCAGATGC TGGCTTTTGT GCACAAGCTG CCCTGCCGGG ACGATGACGG AGTCACCGCC
    AAAGACCTCA GCAAGCAACT GCACTCAAGC GTGCGAACGG GCAACCTGGA GACATGTCTG
    CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC TTCTTCCACC CCGAAAAGGG CACCACACCT
    CTGCATGTGG CTGCCAAGGC AGGTCAGACG CTGCAGGCCG AGCTGCTTGT GGTGTATGGG
    GCTGACCCTG GCTCCCCAGA TGTTAACGGC CGTACACCCA TTGATTATGC CAGGCAGGCG
    GGGCACCATG AGCTTGCGGA ACGGCTAGTC GAGTGCCAGT ACGAGCTCAC TGACCGGTTG
    GCCTTCTATC TCTGTGGGCG CAAGCCGGAT CACAAGAATG GGCATTACAT CATCCCACAG
    ATGGCAGACA GATCTCGGCA AAAGTGCATG TCTCAGAGCC TGGACCTGTC CGAGTTGGCC
    AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC AGCAACCGGC TTTTTGAGGA ACTAGCCATG
    GACGTGTATG ACGAGGTGGA TCGAAGGGAA AATGACGCTG TGTGGCTGGC GACCCAGAAC
    CACAGCACCC TGGTGACCGA GCGCAGCGCC GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC
    TCAGCCACAC GGAATCAGGG GCGACAGAAG CTGGCCCGCT TTAACGCCCG GGAGTTCGCC
    ACCTTGATCA TCGACATCCT CAGCGAGGCC AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC
    AGCCCCACAG ACAACCTCGA GCTGTCCGCG CGGGGCCAGA GTGACCTGGA CGACCAGCAC
    GACTACGACA GCGTGGCCTC GGACGAGGAC ACAGACCAGG AGCCCCTGCG CAGTGCTGGT
    GCCACGCGGA ACAACCGAGC TCGGAGCATG GACTCCTCGG ACCTGTCAGA CGGGGCTGTG
    ACGCTGCAAG AGTACCTGGA GCTGAAAAAG GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG
    CAGCTCATGA AGGTCAATAG CAGCCTGAGC GATGAGCTCC GGAGGCTGCA GAGGGAGATC
    CACAAGCTGC AGGCCGAGAA CCTGCAGATC CGGCAGCCAC CAGGGCCGGT GCCCACACCC
    CCCCTCCCCA GCGAGCGAGC TGAGCACGCA CCCGTGGGGC CTGGCGGGAG CACCCACCGC
    AGGGACCGCC AGGCCTTTTC CATGTATGAA CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC
    CCGCCCGGGG ACGAGCTCAC CACCCGGCTC CAGCCTTTCC ACAGCACCGA GCTGGAGGAC
    GACGCCATCT ATTCAGTGCA CGTCCCTGCC GGCCTTTACC GGATCCGGAA GGGGGTGTCG
    GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC CCACTGCTGT CGTGCTCCCA GGAAGGAAGC
    CGCCACACGA GCAAGCTCTC CCGCCACGGC AGCGGCGCAG ACAGTGACTA TGAGAACACG
    CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA
    GAGGACTTCC ACCCGGAGTT GGAAAGCCTG GATGGCGACC TCGACCCTGG GCTTCCCAGC
    ACGGAGGATG TCATCCTGAA GACGGAGCAG GTCACCAAGA ACATTCAGGA GTTACTGCGG
    GCGGCCCAGG AATTCAAGCA TGACAGCTTC GTGCCCTGCT CAGAGAAGAT CCATTTGGCC
    GTGACTGAGA TGGCCTCTCT CTTCCCGAAG AGGCCCGCCC TGGAGCCCGT GCGAAGCTCG
    CTGCGGCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGCG AGTGCCGGAA GACGGTGCCC
    CCAGAGCCCG GCGCTCCCGT GGACTTCCAG CTGCTGACTC AGCAGGTGAT CCAGTGCGCC
    TATGACATCG CCAAGGCCGC CAAGCAGCTG GTCACCATCA CCACCCGCGA GAAGAAGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006042277.1
    CDS41..2983
    Translation

    Target ORF information:

    RefSeq Version XM_006042215.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006042215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCGGGGAT GCGGCGGAGA GAGGAGGGGG ACGCGGATGC CCTGGGCAGC GCTTGTTATC 
    ACTGGGCCCC GCGGCATCAT GGGGCTTGTA GTCCGCCTCG TGGAACCGGG GCTGGCCCCG
    GCGCGGAACG ATGAAGGAGG AGTGGACTGC CTCGCCCCTT TCTCCAGGCT GGGCGGAGCC
    AGTTGGCGTC TCCCGCTGCC GGGGACCGCT GCCCACCTCC ACCACGAGGG GTCACCAGAG
    TGCCGTGGCT GCAGCCGGAG TTGGGGAGGG GGCTGGAGCG GGGAAGGGAC GGCAGGAGTG
    GCCGGGAGAA TGGGTTACTG TCCCTGGGCC CGAGCTCCTC AGGCCACCCT CAGCCTCGAA
    TATGAAGGGG ACGAGGCTGT GAGGCGAACT CGCAGCGTCT CGGCGGCAGG GGCGCACCGA
    GGCCCGGGCC GCAGGCGGCG CTGCGCGCTC GGGCCTGGCG GCGGCGGCGG CCCCTTTAAA
    GTCCTGTGTG CTCCTGGGAA CCCCCTCCCT CCGCCCCCAC CTCGGGCCCC GATCCAGAAA
    GCAGGTCAGG AGGAGGCAGG TGCTGAGGGG ACTGAGTGTG CAAATTGTGC GTGGAGAGCT
    AAGCACAGTG CAGGTCCAGG GCCGTGCTCG GGAGGCTTAT CCGTCTGGCT GTGGCTGACC
    CTTCTCCCTG CTCACCCCGC AGACCCCGGC TGGGCCTCCA TCAGCAGGGG TGTGCTGGTG
    TGTGACGAGT GCTGCAGCGT GCACCGGAGT CTGGGCCGCC ACATCTCCAT TGTCAAGCAC
    CTCCGCCACA GCGCCTGGCC CCCCACCCTG CTGCAGATGG TGCACACGCT CGCCAGCAAC
    GGGGCCAACT CCATCTGGGA GCACTCCCTG CTGGACCCCG CGCAGGTGCA GAGCGGCCGG
    CGTAAAGCCA ACCCCCAAGA CAAAGTCCAC CCCATCAAGT CTGAGTTCAT CAGGGCCAAG
    TACCAGATGC TGGCTTTTGT GCACAAGCTG CCCTGCCGGG ACGATGACGG AGTCACCGCC
    AAAGACCTCA GCAAGCAACT GCACTCAAGC GTGCGAACGG GCAACCTGGA GACATGTCTG
    CGCCTGCTGT CCCTGGGTGC CCAGGCCAAC TTCTTCCACC CCGAAAAGGG CACCACACCT
    CTGCATGTGG CTGCCAAGGC AGGTCAGACG CTGCAGGCCG AGCTGCTTGT GGTGTATGGG
    GCTGACCCTG GCTCCCCAGA TGTTAACGGC CGTACACCCA TTGATTATGC CAGGCAGGCG
    GGGCACCATG AGCTTGCGGA ACGGCTAGTC GAGTGCCAGT ACGAGCTCAC TGACCGGTTG
    GCCTTCTATC TCTGTGGGCG CAAGCCGGAT CACAAGAATG GGCATTACAT CATCCCACAG
    ATGGCAGACA GATCTCGGCA AAAGTGCATG TCTCAGAGCC TGGACCTGTC CGAGTTGGCC
    AAAGCTGCCA AGAAGAAGCT GCAGGCGCTC AGCAACCGGC TTTTTGAGGA ACTAGCCATG
    GACGTGTATG ACGAGGTGGA TCGAAGGGAA AATGACGCTG TGTGGCTGGC GACCCAGAAC
    CACAGCACCC TGGTGACCGA GCGCAGCGCC GTGCCCTTCC TGCCAGTGAA CCCCGAGTAC
    TCAGCCACAC GGAATCAGGG GCGACAGAAG CTGGCCCGCT TTAACGCCCG GGAGTTCGCC
    ACCTTGATCA TCGACATCCT CAGCGAGGCC AAGAGGAGAC AGCAGGGCAA GAGCCTGGGC
    AGCCCCACAG ACAACCTCGA GCTGTCCGCG CGGGGCCAGA GTGACCTGGA CGACCAGCAC
    GACTACGACA GCGTGGCCTC GGACGAGGAC ACAGACCAGG AGCCCCTGCG CAGTGCTGGT
    GCCACGCGGA ACAACCGAGC TCGGAGCATG GACTCCTCGG ACCTGTCAGA CGGGGCTGTG
    ACGCTGCAAG AGTACCTGGA GCTGAAAAAG GCCCTGGCCG CTTCCGAGGC CAAGGTGCAG
    CAGCTCATGA AGGTCAATAG CAGCCTGAGC GATGAGCTCC GGAGGCTGCA GAGGGAGATC
    CACAAGCTGC AGGCCGAGAA CCTGCAGATC CGGCAGCCAC CAGGGCCGGT GCCCACACCC
    CCCCTCCCCA GCGAGCGAGC TGAGCACGCA CCCGTGGGGC CTGGCGGGAG CACCCACCGC
    AGGGACCGCC AGGCCTTTTC CATGTATGAA CCAGGCTCCG CCCTGAAGCC CTTTGGGGTC
    CCGCCCGGGG ACGAGCTCAC CACCCGGCTC CAGCCTTTCC ACAGCACCGA GCTGGAGGAC
    GACGCCATCT ATTCAGTGCA CGTCCCTGCC GGCCTTTACC GGATCCGGAA GGGGGTGTCG
    GCCTCAGCTG TGCCCTTCAC TCCCTCCTCC CCACTGCTGT CGTGCTCCCA GGAAGGAAGC
    CGCCACACGA GCAAGCTCTC CCGCCACGGC AGCGGCGCAG ACAGTGACTA TGAGAACACG
    CAAAGTGGGG ACCCGCTGCT GGGCCTGGAA GGGAAGAGGT TTCTGGAGCT GGGCAAGGAA
    GAGGACTTCC ACCCGGAGTT GGAAAGCCTG GATGGCGACC TCGACCCTGG GCTTCCCAGC
    ACGGAGGATG TCATCCTGAA GACGGAGCAG GTCACCAAGA ACATTCAGGA GTTACTGCGG
    GCGGCCCAGG AATTCAAGCA TGACAGCTTC GTGCCCTGCT CAGAGAAGAT CCATTTGGCC
    GTGACTGAGA TGGCCTCTCT CTTCCCGAAG AGGCCCGCCC TGGAGCCCGT GCGAAGCTCG
    CTGCGGCTGC TCAACGCCAG CGCCTACCGG CTGCAGAGCG AGTGCCGGAA GACGGTGCCC
    CCAGAGCCCG GCGCTCCCGT GGACTTCCAG CTGCTGACTC AGCAGGTGAT CCAGTGCGCC
    TATGACATCG CCAAGGCCGC CAAGCAGCTG GTCACCATCA CCACCCGCGA GAAGAAGCAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.