PLCL1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCL1
Entrez Gene ID	102408301
Full Name	phospholipase C like 1 (inactive)
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006078169.1	XP_006078231.1	inactive phospholipase C-like protein 1 isoform X1
XM_006078169.2	XP_006078231.1	inactive phospholipase C-like protein 1 isoform X1
XM_025277942.1	XP_025133727.1	inactive phospholipase C-like protein 1 isoform X2
XM_006078171.2	XP_006078233.1	inactive phospholipase C-like protein 1 isoform X5
XM_006078170.2	XP_006078232.1	inactive phospholipase C-like protein 1 isoform X4
XM_006078170.1	XP_006078232.1	inactive phospholipase C-like protein 1 isoform X2
XM_025277943.1	XP_025133728.1	inactive phospholipase C-like protein 1 isoform X3
XM_006078171.1	XP_006078233.1	inactive phospholipase C-like protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following PLCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu31417	XM_006078171.1 Latest version!	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu55753	XM_006078171.2 Latest version!	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu31415	XM_006078169.1 Latest version!	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu31416	XM_006078170.1 Latest version!	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu55749	XM_006078169.2 Latest version!	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu55750	XM_025277942.1 Latest version!	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu55751	XM_025277943.1 Latest version!	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu55752	XM_006078170.2 Latest version!	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCL1 ( XM_006078171.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078233.1 Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu31417
Clone ID Related Accession (Same CDS sequence)	XM_006078171.1
Accession Version	XM_006078171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG AAAAGAAAAT CAGCAGCGCA AGCGACTGCA TCACTTTCAT GCAGGCTGGC 
TGTGAACTGA AGAAAGTGCG GCCGAATTCG CGCATTTACA ACAGGTTCTT CACTCTGGAC 
ACAGACCTGC AGGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTCGAGAA AGCTAAGCTT 
GATATTTCTG CCATAAAAGA GATCCGACTG GGGAAAAACA CGGAAACATT TAGAAACAAT 
GGCCTTGCTG ACCAGATTTG TGAGGATTGT GCCTTTTCCA TACTCCACGG GGAAAACTAC 
GAGTCTCTGG ACTTAGTGGC CAATTCAGCG GACGTAGCAA ACATCTGGGT GTCAGGATTG 
CGGTATCTGG TTTCTCGAAG TAAGCAACCC CTCGATTTTA TGGAGGGCAA CCAGAACACA 
CCAAGGTTCA TGTGGTTGAA GACAGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAACTCA ACCCTACCCT GAAGGAGTCC 
AAGATTAGGC TAAAATTTAA AGAAATCCAG AAGAGCAAAG AAAAACTGAC AACTCGAGTG 
ACGGAAGAGG AGTTTTGCGA AGCTTTTTGT GAACTTTGCA CCAGGCCGGA AGTGTATTTC 
TTACTTGTGC AGATATCAAA AAACAAAGAA TATTTGGATG CCAATGATCT TATGCTTTTT 
TTGGAAGCTG AGCAAGGAGT CACCCATATC ACTGAGGACA TGTGCTTAGA CATTATTCGG 
AGATATGAAC TTTCCGAAGA AGGACGTCAG AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATTTGT TGTCACCAGA ATGTAACATT TTTGATCCTG AACAAAATAA GGTTGTCCAA 
GACATGACCC AGCCTTTATC TCACTATTAC ATCAATGCCT CTCACAACAC CTATCTCATT 
GAAGACCAGT TCAGAGGACC AGCTGATATT AATGGGTATG TTAGAGCTTT GAAAATGGGC 
TGTCGCAGCA TTGAACTTGA TGTAAATGAT GGCTCAGACA ACGAACCGAT CCTTTGTAAT 
CGAAACAACA TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTGG CTTCTGAATA CCCACTCATT CTTTGTTTGG GGAATCACTG CTCCCTACCA 
CAGCAGAAGG TAATGGTCCA ACAGATGAAA AAGGTCTTTG GCAGTAAACT CTATACTGAA 
GCACCTTTGC TGTCAGAATC CTACCTCCCA TCACCAGAAA AATTAAAAAG AATGATCATT 
GTGAAAGGAA AGAAATTGCC TTCTGATACA GATATTTTAG AAGGAGAAGT TACAGATGAA 
GATGAAGAAG CCGAAATGTC TCGAAGGATG TCAGTAGATT ACAATGGTGA GCAGAAACAG 
ATCTTGCTGT GTAGGGAGCT CTCTGACTTG GTATCTATCT GTAAATCTGT TCAGTACAGG 
GATTTTGAAT TATCTATGAA AAGCCAAAAC TATTGGGAAA TTTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAAGTTC 
TTATCACGAA TCTATCCAAG CGCCATGAGA ATTGATTCCA GTAACCTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GACCTCCACA CAGGCTGGTT TCTTCAGAAT GGAGGCTGTG GTTATGTTCT AAGGCCGTCT 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG TGCTTCATAT TAAGATTATC AGTGGTCAGA ACTTCCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGGGATGT CATAGATCCC TATGTGTGTA TAGAGATACA TGGAATTCCA 
GCGGACTGTT CAGAACAAAG AACTAAAACT GTACAACAAA ACAGTGATAA TCCTATTTTT 
GATGAGACAT TTGAGTTTCA AGTGAACCTG CCTGAACTGG CCATGATCCG TTTTGTGGTT 
CTTGATGATG ACTACATTGG GGATGAGTTT ATAGGGCAAT ATACAATACC GTTTGAATGC 
TTGCAGCCTG GATATCGGCA TGTTCCCCTC CGTTCCTTTG TGGGTGACAT CATGGAGCAT 
GTAACTCTTT TTGTCCACAT AGCAATAACT AATAGAAGTG GAGGAGGAAA GCCACAGAAG 
CGCAGCCTTT CAGTGAGAAT GGGGAAGAAA GTTCGGGAGT GTACCATGCT CAGGAATATT 
GGTCTTAAAA ACATAGATGA CATCTTTAAG ATAGCTGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTGTTA AGGAATTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCTT CTCGGCTCAT CACCAGTGAC 
AATACTCCCT CCGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTAGA GCCTCTGGGG 
GCAATTCCAG ATATGCAGAA AAAGATGCTA GCTGCTTATG ATCTGATGAT CCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGACACAGTC CAGGAAAAGA TTGTGCAGTG TCAAAAAGCA 
GGCATGGAGT TCCATGAGGA GCTTCATAAT CTGGGGGCAA AAGAAGGCTT GAAAGGGAGA 
AAACTCAACA AAGCAACTGA GAGCTTTGCT TGGAACATTA CAGTATTGAA GGGCCAGGGA 
GACCTGTTGA AGAACGCCAA GAATGAAGCC ATAGAAAACA TGAAGCAGAT CCAGCTGGCG 
TGCTCGTCTT GCGGCCTCAG TAAAGCTCCC AGCAGTGGTG CTGAGGTGAA GAGTAAGCGC 
AGCCTGGAGG CCATAGAGGA GAAGGAGAGC TGTGAGGACA ATGGGAGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078233.1
CDS	78..3071
Translation	MPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078171.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078171.1

ORF Insert Sequence:

ATGCCGTCGG AAAAGAAAAT CAGCAGCGCA AGCGACTGCA TCACTTTCAT GCAGGCTGGC 
TGTGAACTGA AGAAAGTGCG GCCGAATTCG CGCATTTACA ACAGGTTCTT CACTCTGGAC 
ACAGACCTGC AGGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTCGAGAA AGCTAAGCTT 
GATATTTCTG CCATAAAAGA GATCCGACTG GGGAAAAACA CGGAAACATT TAGAAACAAT 
GGCCTTGCTG ACCAGATTTG TGAGGATTGT GCCTTTTCCA TACTCCACGG GGAAAACTAC 
GAGTCTCTGG ACTTAGTGGC CAATTCAGCG GACGTAGCAA ACATCTGGGT GTCAGGATTG 
CGGTATCTGG TTTCTCGAAG TAAGCAACCC CTCGATTTTA TGGAGGGCAA CCAGAACACA 
CCAAGGTTCA TGTGGTTGAA GACAGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAACTCA ACCCTACCCT GAAGGAGTCC 
AAGATTAGGC TAAAATTTAA AGAAATCCAG AAGAGCAAAG AAAAACTGAC AACTCGAGTG 
ACGGAAGAGG AGTTTTGCGA AGCTTTTTGT GAACTTTGCA CCAGGCCGGA AGTGTATTTC 
TTACTTGTGC AGATATCAAA AAACAAAGAA TATTTGGATG CCAATGATCT TATGCTTTTT 
TTGGAAGCTG AGCAAGGAGT CACCCATATC ACTGAGGACA TGTGCTTAGA CATTATTCGG 
AGATATGAAC TTTCCGAAGA AGGACGTCAG AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATTTGT TGTCACCAGA ATGTAACATT TTTGATCCTG AACAAAATAA GGTTGTCCAA 
GACATGACCC AGCCTTTATC TCACTATTAC ATCAATGCCT CTCACAACAC CTATCTCATT 
GAAGACCAGT TCAGAGGACC AGCTGATATT AATGGGTATG TTAGAGCTTT GAAAATGGGC 
TGTCGCAGCA TTGAACTTGA TGTAAATGAT GGCTCAGACA ACGAACCGAT CCTTTGTAAT 
CGAAACAACA TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTGG CTTCTGAATA CCCACTCATT CTTTGTTTGG GGAATCACTG CTCCCTACCA 
CAGCAGAAGG TAATGGTCCA ACAGATGAAA AAGGTCTTTG GCAGTAAACT CTATACTGAA 
GCACCTTTGC TGTCAGAATC CTACCTCCCA TCACCAGAAA AATTAAAAAG AATGATCATT 
GTGAAAGGAA AGAAATTGCC TTCTGATACA GATATTTTAG AAGGAGAAGT TACAGATGAA 
GATGAAGAAG CCGAAATGTC TCGAAGGATG TCAGTAGATT ACAATGGTGA GCAGAAACAG 
ATCTTGCTGT GTAGGGAGCT CTCTGACTTG GTATCTATCT GTAAATCTGT TCAGTACAGG 
GATTTTGAAT TATCTATGAA AAGCCAAAAC TATTGGGAAA TTTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAAGTTC 
TTATCACGAA TCTATCCAAG CGCCATGAGA ATTGATTCCA GTAACCTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GACCTCCACA CAGGCTGGTT TCTTCAGAAT GGAGGCTGTG GTTATGTTCT AAGGCCGTCT 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG TGCTTCATAT TAAGATTATC AGTGGTCAGA ACTTCCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGGGATGT CATAGATCCC TATGTGTGTA TAGAGATACA TGGAATTCCA 
GCGGACTGTT CAGAACAAAG AACTAAAACT GTACAACAAA ACAGTGATAA TCCTATTTTT 
GATGAGACAT TTGAGTTTCA AGTGAACCTG CCTGAACTGG CCATGATCCG TTTTGTGGTT 
CTTGATGATG ACTACATTGG GGATGAGTTT ATAGGGCAAT ATACAATACC GTTTGAATGC 
TTGCAGCCTG GATATCGGCA TGTTCCCCTC CGTTCCTTTG TGGGTGACAT CATGGAGCAT 
GTAACTCTTT TTGTCCACAT AGCAATAACT AATAGAAGTG GAGGAGGAAA GCCACAGAAG 
CGCAGCCTTT CAGTGAGAAT GGGGAAGAAA GTTCGGGAGT GTACCATGCT CAGGAATATT 
GGTCTTAAAA ACATAGATGA CATCTTTAAG ATAGCTGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTGTTA AGGAATTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCTT CTCGGCTCAT CACCAGTGAC 
AATACTCCCT CCGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTAGA GCCTCTGGGG 
GCAATTCCAG ATATGCAGAA AAAGATGCTA GCTGCTTATG ATCTGATGAT CCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGACACAGTC CAGGAAAAGA TTGTGCAGTG TCAAAAAGCA 
GGCATGGAGT TCCATGAGGA GCTTCATAAT CTGGGGGCAA AAGAAGGCTT GAAAGGGAGA 
AAACTCAACA AAGCAACTGA GAGCTTTGCT TGGAACATTA CAGTATTGAA GGGCCAGGGA 
GACCTGTTGA AGAACGCCAA GAATGAAGCC ATAGAAAACA TGAAGCAGAT CCAGCTGGCG 
TGCTCGTCTT GCGGCCTCAG TAAAGCTCCC AGCAGTGGTG CTGAGGTGAA GAGTAAGCGC 
AGCCTGGAGG CCATAGAGGA GAAGGAGAGC TGTGAGGACA ATGGGAGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_006078171.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078233.1 Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu55753
Clone ID Related Accession (Same CDS sequence)	XM_006078171.2
Accession Version	XM_006078171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG AAAAGAAAAT CAGCAGCGCA AGCGACTGCA TCACTTTCAT GCAGGCTGGC 
TGTGAACTGA AGAAAGTGCG GCCGAATTCG CGCATTTACA ACAGGTTCTT CACTCTGGAC 
ACAGACCTGC AGGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTCGAGAA AGCTAAGCTT 
GATATTTCTG CCATAAAAGA GATCCGACTG GGGAAAAACA CGGAAACATT TAGAAACAAT 
GGCCTTGCTG ACCAGATTTG TGAGGATTGT GCCTTTTCCA TACTCCACGG GGAAAACTAC 
GAGTCTCTGG ACTTAGTGGC CAATTCAGCG GACGTAGCAA ACATCTGGGT GTCAGGATTG 
CGGTATCTGG TTTCTCGAAG TAAGCAACCC CTCGATTTTA TGGAGGGCAA CCAGAACACA 
CCAAGGTTCA TGTGGTTGAA GACAGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAACTCA ACCCTACTCT GAAGGAGTCC 
AAGATTAGGC TAAAATTTAA AGAAATCCAG AAGAGCAAAG AAAAACTGAC AACTCGAGTG 
ACGGAAGAGG AGTTTTGCGA AGCTTTTTGT GAACTTTGCA CCAGGCCGGA AGTGTATTTC 
TTACTTGTGC AGATATCAAA AAACAAAGAA TATTTGGATG CCAATGATCT TATGCTTTTT 
TTGGAAGCTG AGCAAGGAGT CACCCATATC ACTGAGGACA TGTGCTTAGA CATTATTCGG 
AGATATGAAC TTTCCGAAGA AGGACGTCAG AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATTTGT TGTCACCAGA ATGTAACATT TTTGATCCTG AACAAAATAA GGTTGTCCAA 
GACATGACCC AGCCTTTATC TCACTATTAC ATCAATGCCT CTCACAACAC CTATCTCATT 
GAAGACCAGT TCAGAGGACC AGCTGATATT AATGGGTATG TCAGAGCTTT GAAAATGGGC 
TGTCGCAGCA TTGAACTTGA TGTAAATGAT GGCTCAGACA ACGAACCGAT CCTTTGTAAT 
CGAAACAACA TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTGG CTTCTGAATA CCCACTCATT CTTTGTTTGG GGAATCACTG CTCCCTACCA 
CAGCAGAAGG TAATGGTCCA ACAGATGAAA AAGGTCTTTG GCAGTAAACT CTATACTGAA 
GCACCTTTGC TGTCAGAATC CTACCTCCCA TCACCAGAAA AATTAAAAAG AATGATCATT 
GTGAAAGGAA AGAAATTGCC TTCTGATACA GATATTTTAG AAGGAGAAGT TACAGATGAA 
GATGAAGAAG CCGAAATGTC TCGAAGGATG TCAGTAGATT ACAATGGTGA GCAGAAACAG 
ATCTTGCTGT GTAGGGAGCT CTCTGACTTG GTATCTATCT GTAAATCTGT TCAGTACAGG 
GATTTTGAAT TATCTATGAA AAGCCAAAAC TATTGGGAAA TTTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAAGTTC 
TTATCACGAA TCTATCCAAG CGCCATGAGA ATTGATTCCA GTAACCTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GACCTCCACA CAGGCTGGTT TCTTCAGAAT GGAGGCTGTG GTTATGTTCT AAGGCCGTCT 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG TGCTTCATAT TAAGATTATC AGTGGTCAGA ACTTCCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGGGATGT CATAGATCCC TATGTGTGTA TAGAGATACA TGGAATTCCA 
GCGGACTGTT CAGAACAAAG AACTAAAACT GTACAACAAA ACAGTGATAA TCCTATTTTT 
GATGAGACAT TTGAGTTTCA AGTGAACCTG CCTGAACTGG CCATGATCCG TTTTGTGGTT 
CTTGATGATG ACTACATTGG GGATGAGTTT ATAGGGCAAT ATACAATACC GTTTGAATGC 
TTGCAGCCTG GATATCGGCA TGTTCCCCTC CGTTCCTTTG TGGGTGACAT CATGGAGCAT 
GTAACTCTTT TTGTCCACAT AGCAATAACT AATAGAAGTG GAGGAGGAAA GCCACAGAAG 
CGCAGCCTTT CAGTGAGAAT GGGGAAGAAA GTTCGGGAGT GTACCATGCT CAGGAATATT 
GGTCTTAAAA ACATAGATGA CATCTTTAAG ATAGCTGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTGTTA AGGAATTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCTT CTCGGCTCAT CACCAGTGAC 
AATACTCCCT CCGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTAGA GCCTCTGGGG 
GCAATTCCAG ATATGCAGAA AAAGATGCTA GCTGCTTATG ATCTGATGAT CCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGACACAGTC CAGGAAAAGA TTGTGCAGTG TCAAAAAGCA 
GGCATGGAGT TCCATGAGGA GCTTCATAAT CTGGGGGCAA AAGAAGGCTT GAAAGGGAGA 
AAACTCAACA AAGCAACTGA GAGCTTTGCT TGGAACATTA CAGTATTGAA GGGCCAGGGA 
GACCTGTTGA AGAACGCCAA GAATGAAGCC ATAGAAAACA TGAAGCAGAT CCAGCTGGCG 
TGCTCGTCTT GCGGCCTCAG TAAAGCTCCC AGCAGTGGTG CTGAGGTGAA GAGTAAGCGC 
AGCCTGGAGG CCATAGAGGA GAAGGAGAGC TGTGAGGACA ATGGGAGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078233.1
CDS	2041..5034
Translation	MPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078171.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078171.2

ORF Insert Sequence:

ATGCCGTCGG AAAAGAAAAT CAGCAGCGCA AGCGACTGCA TCACTTTCAT GCAGGCTGGC 
TGTGAACTGA AGAAAGTGCG GCCGAATTCG CGCATTTACA ACAGGTTCTT CACTCTGGAC 
ACAGACCTGC AGGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTCGAGAA AGCTAAGCTT 
GATATTTCTG CCATAAAAGA GATCCGACTG GGGAAAAACA CGGAAACATT TAGAAACAAT 
GGCCTTGCTG ACCAGATTTG TGAGGATTGT GCCTTTTCCA TACTCCACGG GGAAAACTAC 
GAGTCTCTGG ACTTAGTGGC CAATTCAGCG GACGTAGCAA ACATCTGGGT GTCAGGATTG 
CGGTATCTGG TTTCTCGAAG TAAGCAACCC CTCGATTTTA TGGAGGGCAA CCAGAACACA 
CCAAGGTTCA TGTGGTTGAA GACAGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAACTCA ACCCTACTCT GAAGGAGTCC 
AAGATTAGGC TAAAATTTAA AGAAATCCAG AAGAGCAAAG AAAAACTGAC AACTCGAGTG 
ACGGAAGAGG AGTTTTGCGA AGCTTTTTGT GAACTTTGCA CCAGGCCGGA AGTGTATTTC 
TTACTTGTGC AGATATCAAA AAACAAAGAA TATTTGGATG CCAATGATCT TATGCTTTTT 
TTGGAAGCTG AGCAAGGAGT CACCCATATC ACTGAGGACA TGTGCTTAGA CATTATTCGG 
AGATATGAAC TTTCCGAAGA AGGACGTCAG AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATTTGT TGTCACCAGA ATGTAACATT TTTGATCCTG AACAAAATAA GGTTGTCCAA 
GACATGACCC AGCCTTTATC TCACTATTAC ATCAATGCCT CTCACAACAC CTATCTCATT 
GAAGACCAGT TCAGAGGACC AGCTGATATT AATGGGTATG TCAGAGCTTT GAAAATGGGC 
TGTCGCAGCA TTGAACTTGA TGTAAATGAT GGCTCAGACA ACGAACCGAT CCTTTGTAAT 
CGAAACAACA TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTGG CTTCTGAATA CCCACTCATT CTTTGTTTGG GGAATCACTG CTCCCTACCA 
CAGCAGAAGG TAATGGTCCA ACAGATGAAA AAGGTCTTTG GCAGTAAACT CTATACTGAA 
GCACCTTTGC TGTCAGAATC CTACCTCCCA TCACCAGAAA AATTAAAAAG AATGATCATT 
GTGAAAGGAA AGAAATTGCC TTCTGATACA GATATTTTAG AAGGAGAAGT TACAGATGAA 
GATGAAGAAG CCGAAATGTC TCGAAGGATG TCAGTAGATT ACAATGGTGA GCAGAAACAG 
ATCTTGCTGT GTAGGGAGCT CTCTGACTTG GTATCTATCT GTAAATCTGT TCAGTACAGG 
GATTTTGAAT TATCTATGAA AAGCCAAAAC TATTGGGAAA TTTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAAGTTC 
TTATCACGAA TCTATCCAAG CGCCATGAGA ATTGATTCCA GTAACCTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GACCTCCACA CAGGCTGGTT TCTTCAGAAT GGAGGCTGTG GTTATGTTCT AAGGCCGTCT 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG TGCTTCATAT TAAGATTATC AGTGGTCAGA ACTTCCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGGGATGT CATAGATCCC TATGTGTGTA TAGAGATACA TGGAATTCCA 
GCGGACTGTT CAGAACAAAG AACTAAAACT GTACAACAAA ACAGTGATAA TCCTATTTTT 
GATGAGACAT TTGAGTTTCA AGTGAACCTG CCTGAACTGG CCATGATCCG TTTTGTGGTT 
CTTGATGATG ACTACATTGG GGATGAGTTT ATAGGGCAAT ATACAATACC GTTTGAATGC 
TTGCAGCCTG GATATCGGCA TGTTCCCCTC CGTTCCTTTG TGGGTGACAT CATGGAGCAT 
GTAACTCTTT TTGTCCACAT AGCAATAACT AATAGAAGTG GAGGAGGAAA GCCACAGAAG 
CGCAGCCTTT CAGTGAGAAT GGGGAAGAAA GTTCGGGAGT GTACCATGCT CAGGAATATT 
GGTCTTAAAA ACATAGATGA CATCTTTAAG ATAGCTGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTGTTA AGGAATTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCTT CTCGGCTCAT CACCAGTGAC 
AATACTCCCT CCGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTAGA GCCTCTGGGG 
GCAATTCCAG ATATGCAGAA AAAGATGCTA GCTGCTTATG ATCTGATGAT CCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGACACAGTC CAGGAAAAGA TTGTGCAGTG TCAAAAAGCA 
GGCATGGAGT TCCATGAGGA GCTTCATAAT CTGGGGGCAA AAGAAGGCTT GAAAGGGAGA 
AAACTCAACA AAGCAACTGA GAGCTTTGCT TGGAACATTA CAGTATTGAA GGGCCAGGGA 
GACCTGTTGA AGAACGCCAA GAATGAAGCC ATAGAAAACA TGAAGCAGAT CCAGCTGGCG 
TGCTCGTCTT GCGGCCTCAG TAAAGCTCCC AGCAGTGGTG CTGAGGTGAA GAGTAAGCGC 
AGCCTGGAGG CCATAGAGGA GAAGGAGAGC TGTGAGGACA ATGGGAGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_006078169.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078231.1 Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu31415
Clone ID Related Accession (Same CDS sequence)	XM_006078169.1
Accession Version	XM_006078169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACCCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTTA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGATGATCCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA CACAGTCCAG GAAAAGATTG TGCAGTGTCA AAAAGCAGGC 
ATGGAGTTCC ATGAGGAGCT TCATAATCTG GGGGCAAAAG AAGGCTTGAA AGGGAGAAAA 
CTCAACAAAG CAACTGAGAG CTTTGCTTGG AACATTACAG TATTGAAGGG CCAGGGAGAC 
CTGTTGAAGA ACGCCAAGAA TGAAGCCATA GAAAACATGA AGCAGATCCA GCTGGCGTGC 
TCGTCTTGCG GCCTCAGTAA AGCTCCCAGC AGTGGTGCTG AGGTGAAGAG TAAGCGCAGC 
CTGGAGGCCA TAGAGGAGAA GGAGAGCTGT GAGGACAATG GGAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078231.1
CDS	363..3653
Translation	MAEGAASREGSAPPDAAGSEDDPRVGPDVTSGDCVAAAPGGRWRDRRSGVALPGAAGPPADSEAALLEAARATPRRSSIIKDPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078169.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078169.1

ORF Insert Sequence:

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACCCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTTA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGATGATCCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA CACAGTCCAG GAAAAGATTG TGCAGTGTCA AAAAGCAGGC 
ATGGAGTTCC ATGAGGAGCT TCATAATCTG GGGGCAAAAG AAGGCTTGAA AGGGAGAAAA 
CTCAACAAAG CAACTGAGAG CTTTGCTTGG AACATTACAG TATTGAAGGG CCAGGGAGAC 
CTGTTGAAGA ACGCCAAGAA TGAAGCCATA GAAAACATGA AGCAGATCCA GCTGGCGTGC 
TCGTCTTGCG GCCTCAGTAA AGCTCCCAGC AGTGGTGCTG AGGTGAAGAG TAAGCGCAGC 
CTGGAGGCCA TAGAGGAGAA GGAGAGCTGT GAGGACAATG GGAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_006078170.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078232.1 Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu31416
Clone ID Related Accession (Same CDS sequence)	XM_006078170.1
Accession Version	XM_006078170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785813.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGC TGACTAGCTC GCAACCCCTA CTGTCTACTC GGAAAAAAGT GATTGCAGAT 
CCTGCAAACC AAAAATGTGG TGGAAGAAAG AAAACCGTGT CTTTCAGCAG CATGCCGTCG 
GAAAAGAAAA TCAGCAGCGC AAGCGACTGC ATCACTTTCA TGCAGGCTGG CTGTGAACTG 
AAGAAAGTGC GGCCGAATTC GCGCATTTAC AACAGGTTCT TCACTCTGGA CACAGACCTG 
CAGGCTCTTC GCTGGGAACC TTCCAAGAAA GACCTCGAGA AAGCTAAGCT TGATATTTCT 
GCCATAAAAG AGATCCGACT GGGGAAAAAC ACGGAAACAT TTAGAAACAA TGGCCTTGCT 
GACCAGATTT GTGAGGATTG TGCCTTTTCC ATACTCCACG GGGAAAACTA CGAGTCTCTG 
GACTTAGTGG CCAATTCAGC GGACGTAGCA AACATCTGGG TGTCAGGATT GCGGTATCTG 
GTTTCTCGAA GTAAGCAACC CCTCGATTTT ATGGAGGGCA ACCAGAACAC ACCAAGGTTC 
ATGTGGTTGA AGACAGTGTT TGAAGCAGCA GATATCGATG GGAATGGGAT TATGTTGGAA 
GACACCTCTG TAGAGTTAAT AAAACAACTC AACCCTACCC TGAAGGAGTC CAAGATTAGG 
CTAAAATTTA AAGAAATCCA GAAGAGCAAA GAAAAACTGA CAACTCGAGT GACGGAAGAG 
GAGTTTTGCG AAGCTTTTTG TGAACTTTGC ACCAGGCCGG AAGTGTATTT CTTACTTGTG 
CAGATATCAA AAAACAAAGA ATATTTGGAT GCCAATGATC TTATGCTTTT TTTGGAAGCT 
GAGCAAGGAG TCACCCATAT CACTGAGGAC ATGTGCTTAG ACATTATTCG GAGATATGAA 
CTTTCCGAAG AAGGACGTCA GAAAGGGTTT CTTGCAATTG ATGGCTTTAC CCAGTATTTG 
TTGTCACCAG AATGTAACAT TTTTGATCCT GAACAAAATA AGGTTGTCCA AGACATGACC 
CAGCCTTTAT CTCACTATTA CATCAATGCC TCTCACAACA CCTATCTCAT TGAAGACCAG 
TTCAGAGGAC CAGCTGATAT TAATGGGTAT GTTAGAGCTT TGAAAATGGG CTGTCGCAGC 
ATTGAACTTG ATGTAAATGA TGGCTCAGAC AACGAACCGA TCCTTTGTAA TCGAAACAAC 
ATGACAACAC ACCTTTCCTT TCGAAGTGTC ATAGAGGTGA TAAATAAATT TGCCTTTGTG 
GCTTCTGAAT ACCCACTCAT TCTTTGTTTG GGGAATCACT GCTCCCTACC ACAGCAGAAG 
GTAATGGTCC AACAGATGAA AAAGGTCTTT GGCAGTAAAC TCTATACTGA AGCACCTTTG 
CTGTCAGAAT CCTACCTCCC ATCACCAGAA AAATTAAAAA GAATGATCAT TGTGAAAGGA 
AAGAAATTGC CTTCTGATAC AGATATTTTA GAAGGAGAAG TTACAGATGA AGATGAAGAA 
GCCGAAATGT CTCGAAGGAT GTCAGTAGAT TACAATGGTG AGCAGAAACA GATCTTGCTG 
TGTAGGGAGC TCTCTGACTT GGTATCTATC TGTAAATCTG TTCAGTACAG GGATTTTGAA 
TTATCTATGA AAAGCCAAAA CTATTGGGAA ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC 
CGAATTGCAA ATGAATACCC AGAGGATTTT GTAAATTATA ATAAGAAGTT CTTATCACGA 
ATCTATCCAA GCGCCATGAG AATTGATTCC AGTAACCTGA ATCCACAGGA CTTTTGGAAT 
TGTGGCTGTC AGATTGTGGC AATGAATTTT CAGACTCCAG GTCCAATGAT GGACCTCCAC 
ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT GGTTATGTTC TAAGGCCGTC TATCATGCGA 
GATGAAGTTT CTTACTTCAG TGCAAACACA AAGGGCATTG TACCTGGGGT GTCTCCTCTA 
GTGCTTCATA TTAAGATTAT CAGTGGTCAG AACTTCCCAA AGCCCAAGGG AGCTTGTGCC 
AAAGGGGATG TCATAGATCC CTATGTGTGT ATAGAGATAC ATGGAATTCC AGCGGACTGT 
TCAGAACAAA GAACTAAAAC TGTACAACAA AACAGTGATA ATCCTATTTT TGATGAGACA 
TTTGAGTTTC AAGTGAACCT GCCTGAACTG GCCATGATCC GTTTTGTGGT TCTTGATGAT 
GACTACATTG GGGATGAGTT TATAGGGCAA TATACAATAC CGTTTGAATG CTTGCAGCCT 
GGATATCGGC ATGTTCCCCT CCGTTCCTTT GTGGGTGACA TCATGGAGCA TGTAACTCTT 
TTTGTCCACA TAGCAATAAC TAATAGAAGT GGAGGAGGAA AGCCACAGAA GCGCAGCCTT 
TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG TGTACCATGC TCAGGAATAT TGGTCTTAAA 
AACATAGATG ACATCTTTAA GATAGCTGTT CATCCCTTAC GAGAAGCCAT AGATATGAGA 
GAAAATATGC AGAATGCCAT AGTGTCTGTT AAGGAATTGT GTGGACTTCC TCCAATTGCC 
AGTCTGAAGC AGTGCCTGTT GACCCTGTCT TCTCGGCTCA TCACCAGTGA CAATACTCCC 
TCCGTCTCTC TTGTGATGAA AGACAACTTT CCTTATCTAG AGCCTCTGGG GGCAATTCCA 
GATATGCAGA AAAAGATGCT AGCTGCTTAT GATCTGATGA TCCAAGAGAG CCGATTTCTC 
ATAGAAATGG CAGACACAGT CCAGGAAAAG ATTGTGCAGT GTCAAAAAGC AGGCATGGAG 
TTCCATGAGG AGCTTCATAA TCTGGGGGCA AAAGAAGGCT TGAAAGGGAG AAAACTCAAC 
AAAGCAACTG AGAGCTTTGC TTGGAACATT ACAGTATTGA AGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078232.1
CDS	12..3116
Translation	MERLTSSQPLLSTRKKVIADPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078170.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078170.1

ORF Insert Sequence:

ATGGAAAGGC TGACTAGCTC GCAACCCCTA CTGTCTACTC GGAAAAAAGT GATTGCAGAT 
CCTGCAAACC AAAAATGTGG TGGAAGAAAG AAAACCGTGT CTTTCAGCAG CATGCCGTCG 
GAAAAGAAAA TCAGCAGCGC AAGCGACTGC ATCACTTTCA TGCAGGCTGG CTGTGAACTG 
AAGAAAGTGC GGCCGAATTC GCGCATTTAC AACAGGTTCT TCACTCTGGA CACAGACCTG 
CAGGCTCTTC GCTGGGAACC TTCCAAGAAA GACCTCGAGA AAGCTAAGCT TGATATTTCT 
GCCATAAAAG AGATCCGACT GGGGAAAAAC ACGGAAACAT TTAGAAACAA TGGCCTTGCT 
GACCAGATTT GTGAGGATTG TGCCTTTTCC ATACTCCACG GGGAAAACTA CGAGTCTCTG 
GACTTAGTGG CCAATTCAGC GGACGTAGCA AACATCTGGG TGTCAGGATT GCGGTATCTG 
GTTTCTCGAA GTAAGCAACC CCTCGATTTT ATGGAGGGCA ACCAGAACAC ACCAAGGTTC 
ATGTGGTTGA AGACAGTGTT TGAAGCAGCA GATATCGATG GGAATGGGAT TATGTTGGAA 
GACACCTCTG TAGAGTTAAT AAAACAACTC AACCCTACCC TGAAGGAGTC CAAGATTAGG 
CTAAAATTTA AAGAAATCCA GAAGAGCAAA GAAAAACTGA CAACTCGAGT GACGGAAGAG 
GAGTTTTGCG AAGCTTTTTG TGAACTTTGC ACCAGGCCGG AAGTGTATTT CTTACTTGTG 
CAGATATCAA AAAACAAAGA ATATTTGGAT GCCAATGATC TTATGCTTTT TTTGGAAGCT 
GAGCAAGGAG TCACCCATAT CACTGAGGAC ATGTGCTTAG ACATTATTCG GAGATATGAA 
CTTTCCGAAG AAGGACGTCA GAAAGGGTTT CTTGCAATTG ATGGCTTTAC CCAGTATTTG 
TTGTCACCAG AATGTAACAT TTTTGATCCT GAACAAAATA AGGTTGTCCA AGACATGACC 
CAGCCTTTAT CTCACTATTA CATCAATGCC TCTCACAACA CCTATCTCAT TGAAGACCAG 
TTCAGAGGAC CAGCTGATAT TAATGGGTAT GTTAGAGCTT TGAAAATGGG CTGTCGCAGC 
ATTGAACTTG ATGTAAATGA TGGCTCAGAC AACGAACCGA TCCTTTGTAA TCGAAACAAC 
ATGACAACAC ACCTTTCCTT TCGAAGTGTC ATAGAGGTGA TAAATAAATT TGCCTTTGTG 
GCTTCTGAAT ACCCACTCAT TCTTTGTTTG GGGAATCACT GCTCCCTACC ACAGCAGAAG 
GTAATGGTCC AACAGATGAA AAAGGTCTTT GGCAGTAAAC TCTATACTGA AGCACCTTTG 
CTGTCAGAAT CCTACCTCCC ATCACCAGAA AAATTAAAAA GAATGATCAT TGTGAAAGGA 
AAGAAATTGC CTTCTGATAC AGATATTTTA GAAGGAGAAG TTACAGATGA AGATGAAGAA 
GCCGAAATGT CTCGAAGGAT GTCAGTAGAT TACAATGGTG AGCAGAAACA GATCTTGCTG 
TGTAGGGAGC TCTCTGACTT GGTATCTATC TGTAAATCTG TTCAGTACAG GGATTTTGAA 
TTATCTATGA AAAGCCAAAA CTATTGGGAA ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC 
CGAATTGCAA ATGAATACCC AGAGGATTTT GTAAATTATA ATAAGAAGTT CTTATCACGA 
ATCTATCCAA GCGCCATGAG AATTGATTCC AGTAACCTGA ATCCACAGGA CTTTTGGAAT 
TGTGGCTGTC AGATTGTGGC AATGAATTTT CAGACTCCAG GTCCAATGAT GGACCTCCAC 
ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT GGTTATGTTC TAAGGCCGTC TATCATGCGA 
GATGAAGTTT CTTACTTCAG TGCAAACACA AAGGGCATTG TACCTGGGGT GTCTCCTCTA 
GTGCTTCATA TTAAGATTAT CAGTGGTCAG AACTTCCCAA AGCCCAAGGG AGCTTGTGCC 
AAAGGGGATG TCATAGATCC CTATGTGTGT ATAGAGATAC ATGGAATTCC AGCGGACTGT 
TCAGAACAAA GAACTAAAAC TGTACAACAA AACAGTGATA ATCCTATTTT TGATGAGACA 
TTTGAGTTTC AAGTGAACCT GCCTGAACTG GCCATGATCC GTTTTGTGGT TCTTGATGAT 
GACTACATTG GGGATGAGTT TATAGGGCAA TATACAATAC CGTTTGAATG CTTGCAGCCT 
GGATATCGGC ATGTTCCCCT CCGTTCCTTT GTGGGTGACA TCATGGAGCA TGTAACTCTT 
TTTGTCCACA TAGCAATAAC TAATAGAAGT GGAGGAGGAA AGCCACAGAA GCGCAGCCTT 
TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG TGTACCATGC TCAGGAATAT TGGTCTTAAA 
AACATAGATG ACATCTTTAA GATAGCTGTT CATCCCTTAC GAGAAGCCAT AGATATGAGA 
GAAAATATGC AGAATGCCAT AGTGTCTGTT AAGGAATTGT GTGGACTTCC TCCAATTGCC 
AGTCTGAAGC AGTGCCTGTT GACCCTGTCT TCTCGGCTCA TCACCAGTGA CAATACTCCC 
TCCGTCTCTC TTGTGATGAA AGACAACTTT CCTTATCTAG AGCCTCTGGG GGCAATTCCA 
GATATGCAGA AAAAGATGCT AGCTGCTTAT GATCTGATGA TCCAAGAGAG CCGATTTCTC 
ATAGAAATGG CAGACACAGT CCAGGAAAAG ATTGTGCAGT GTCAAAAAGC AGGCATGGAG 
TTCCATGAGG AGCTTCATAA TCTGGGGGCA AAAGAAGGCT TGAAAGGGAG AAAACTCAAC 
AAAGCAACTG AGAGCTTTGC TTGGAACATT ACAGTATTGA AGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_006078169.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078231.1 Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.

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CloneID	OBu55749
Clone ID Related Accession (Same CDS sequence)	XM_006078169.2
Accession Version	XM_006078169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACTCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTCA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGATGATCCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA CACAGTCCAG GAAAAGATTG TGCAGTGTCA AAAAGCAGGC 
ATGGAGTTCC ATGAGGAGCT TCATAATCTG GGGGCAAAAG AAGGCTTGAA AGGGAGAAAA 
CTCAACAAAG CAACTGAGAG CTTTGCTTGG AACATTACAG TATTGAAGGG CCAGGGAGAC 
CTGTTGAAGA ACGCCAAGAA TGAAGCCATA GAAAACATGA AGCAGATCCA GCTGGCGTGC 
TCGTCTTGCG GCCTCAGTAA AGCTCCCAGC AGTGGTGCTG AGGTGAAGAG TAAGCGCAGC 
CTGGAGGCCA TAGAGGAGAA GGAGAGCTGT GAGGACAATG GGAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078231.1
CDS	467..3757
Translation	MAEGAASREGSAPPDAAGSEDDPRVGPDVTSGDCVAAAPGGRWRDRRSGVALPGAAGPPADSEAALLEAARATPRRSSIIKDPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078169.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078169.2

ORF Insert Sequence:

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACTCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTCA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGATGATCCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA CACAGTCCAG GAAAAGATTG TGCAGTGTCA AAAAGCAGGC 
ATGGAGTTCC ATGAGGAGCT TCATAATCTG GGGGCAAAAG AAGGCTTGAA AGGGAGAAAA 
CTCAACAAAG CAACTGAGAG CTTTGCTTGG AACATTACAG TATTGAAGGG CCAGGGAGAC 
CTGTTGAAGA ACGCCAAGAA TGAAGCCATA GAAAACATGA AGCAGATCCA GCTGGCGTGC 
TCGTCTTGCG GCCTCAGTAA AGCTCCCAGC AGTGGTGCTG AGGTGAAGAG TAAGCGCAGC 
CTGGAGGCCA TAGAGGAGAA GGAGAGCTGT GAGGACAATG GGAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_025277942.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133727.1 Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.

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CloneID	OBu55750
Clone ID Related Accession (Same CDS sequence)	XM_025277942.1
Accession Version	XM_025277942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACTCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTCA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133727.1
CDS	471..3575
Translation	MAEGAASREGSAPPDAAGSEDDPRVGPDVTSGDCVAAAPGGRWRDRRSGVALPGAAGPPADSEAALLEAARATPRRSSIIKDPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_025277942.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025277942.1

ORF Insert Sequence:

ATGGCTGAGG GCGCAGCCAG CAGGGAGGGT TCAGCGCCGC CTGACGCGGC CGGGAGCGAA 
GACGACCCCC GAGTGGGCCC GGATGTCACC TCCGGGGACT GTGTGGCGGC GGCCCCTGGG 
GGCCGGTGGA GGGACCGTCG CAGTGGGGTC GCCCTGCCCG GAGCTGCGGG GCCCCCGGCG 
GACAGTGAGG CCGCCCTCCT GGAGGCTGCT CGGGCGACCC CTCGGCGCAG CAGCATCATC 
AAGGATCCTG CAAACCAAAA ATGTGGTGGA AGAAAGAAAA CCGTGTCTTT CAGCAGCATG 
CCGTCGGAAA AGAAAATCAG CAGCGCAAGC GACTGCATCA CTTTCATGCA GGCTGGCTGT 
GAACTGAAGA AAGTGCGGCC GAATTCGCGC ATTTACAACA GGTTCTTCAC TCTGGACACA 
GACCTGCAGG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TCGAGAAAGC TAAGCTTGAT 
ATTTCTGCCA TAAAAGAGAT CCGACTGGGG AAAAACACGG AAACATTTAG AAACAATGGC 
CTTGCTGACC AGATTTGTGA GGATTGTGCC TTTTCCATAC TCCACGGGGA AAACTACGAG 
TCTCTGGACT TAGTGGCCAA TTCAGCGGAC GTAGCAAACA TCTGGGTGTC AGGATTGCGG 
TATCTGGTTT CTCGAAGTAA GCAACCCCTC GATTTTATGG AGGGCAACCA GAACACACCA 
AGGTTCATGT GGTTGAAGAC AGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAACTCAACC CTACTCTGAA GGAGTCCAAG 
ATTAGGCTAA AATTTAAAGA AATCCAGAAG AGCAAAGAAA AACTGACAAC TCGAGTGACG 
GAAGAGGAGT TTTGCGAAGC TTTTTGTGAA CTTTGCACCA GGCCGGAAGT GTATTTCTTA 
CTTGTGCAGA TATCAAAAAA CAAAGAATAT TTGGATGCCA ATGATCTTAT GCTTTTTTTG 
GAAGCTGAGC AAGGAGTCAC CCATATCACT GAGGACATGT GCTTAGACAT TATTCGGAGA 
TATGAACTTT CCGAAGAAGG ACGTCAGAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATTTGTTGT CACCAGAATG TAACATTTTT GATCCTGAAC AAAATAAGGT TGTCCAAGAC 
ATGACCCAGC CTTTATCTCA CTATTACATC AATGCCTCTC ACAACACCTA TCTCATTGAA 
GACCAGTTCA GAGGACCAGC TGATATTAAT GGGTATGTCA GAGCTTTGAA AATGGGCTGT 
CGCAGCATTG AACTTGATGT AAATGATGGC TCAGACAACG AACCGATCCT TTGTAATCGA 
AACAACATGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTGGCTT CTGAATACCC ACTCATTCTT TGTTTGGGGA ATCACTGCTC CCTACCACAG 
CAGAAGGTAA TGGTCCAACA GATGAAAAAG GTCTTTGGCA GTAAACTCTA TACTGAAGCA 
CCTTTGCTGT CAGAATCCTA CCTCCCATCA CCAGAAAAAT TAAAAAGAAT GATCATTGTG 
AAAGGAAAGA AATTGCCTTC TGATACAGAT ATTTTAGAAG GAGAAGTTAC AGATGAAGAT 
GAAGAAGCCG AAATGTCTCG AAGGATGTCA GTAGATTACA ATGGTGAGCA GAAACAGATC 
TTGCTGTGTA GGGAGCTCTC TGACTTGGTA TCTATCTGTA AATCTGTTCA GTACAGGGAT 
TTTGAATTAT CTATGAAAAG CCAAAACTAT TGGGAAATTT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAAGTTCTTA 
TCACGAATCT ATCCAAGCGC CATGAGAATT GATTCCAGTA ACCTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAC 
CTCCACACAG GCTGGTTTCT TCAGAATGGA GGCTGTGGTT ATGTTCTAAG GCCGTCTATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGTGC TTCATATTAA GATTATCAGT GGTCAGAACT TCCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GGGATGTCAT AGATCCCTAT GTGTGTATAG AGATACATGG AATTCCAGCG 
GACTGTTCAG AACAAAGAAC TAAAACTGTA CAACAAAACA GTGATAATCC TATTTTTGAT 
GAGACATTTG AGTTTCAAGT GAACCTGCCT GAACTGGCCA TGATCCGTTT TGTGGTTCTT 
GATGATGACT ACATTGGGGA TGAGTTTATA GGGCAATATA CAATACCGTT TGAATGCTTG 
CAGCCTGGAT ATCGGCATGT TCCCCTCCGT TCCTTTGTGG GTGACATCAT GGAGCATGTA 
ACTCTTTTTG TCCACATAGC AATAACTAAT AGAAGTGGAG GAGGAAAGCC ACAGAAGCGC 
AGCCTTTCAG TGAGAATGGG GAAGAAAGTT CGGGAGTGTA CCATGCTCAG GAATATTGGT 
CTTAAAAACA TAGATGACAT CTTTAAGATA GCTGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTGTTAAGG AATTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCTTCTC GGCTCATCAC CAGTGACAAT 
ACTCCCTCCG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTAGAGCC TCTGGGGGCA 
ATTCCAGATA TGCAGAAAAA GATGCTAGCT GCTTATGATC TGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_025277943.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025133728.1 Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.

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CloneID	OBu55751
Clone ID Related Accession (Same CDS sequence)	XM_025277943.1
Accession Version	XM_025277943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCTC CGGGGACTGT GTGGCGGCGG CCCCTGGGGG CCGGTGGAGG GACCGTCGCA 
GTGGGGTCGC CCTGCCCGGA GCTGCGGGGC CCCCGGCGGA CAGTGAGGCC GCCCTCCTGG 
AGGCTGCTCG GGCGACCCCT CGGCGCAGCA GCATCATCAA GGCGGATTCT TTACCAGCTG 
AGCTACCAGG GAAGTCCTAA AAATAAGGAT CCTGCAAACC AAAAATGTGG TGGAAGAAAG 
AAAACCGTGT CTTTCAGCAG CATGCCGTCG GAAAAGAAAA TCAGCAGCGC AAGCGACTGC 
ATCACTTTCA TGCAGGCTGG CTGTGAACTG AAGAAAGTGC GGCCGAATTC GCGCATTTAC 
AACAGGTTCT TCACTCTGGA CACAGACCTG CAGGCTCTTC GCTGGGAACC TTCCAAGAAA 
GACCTCGAGA AAGCTAAGCT TGATATTTCT GCCATAAAAG AGATCCGACT GGGGAAAAAC 
ACGGAAACAT TTAGAAACAA TGGCCTTGCT GACCAGATTT GTGAGGATTG TGCCTTTTCC 
ATACTCCACG GGGAAAACTA CGAGTCTCTG GACTTAGTGG CCAATTCAGC GGACGTAGCA 
AACATCTGGG TGTCAGGATT GCGGTATCTG GTTTCTCGAA GTAAGCAACC CCTCGATTTT 
ATGGAGGGCA ACCAGAACAC ACCAAGGTTC ATGTGGTTGA AGACAGTGTT TGAAGCAGCA 
GATATCGATG GGAATGGGAT TATGTTGGAA GACACCTCTG TAGAGTTAAT AAAACAACTC 
AACCCTACTC TGAAGGAGTC CAAGATTAGG CTAAAATTTA AAGAAATCCA GAAGAGCAAA 
GAAAAACTGA CAACTCGAGT GACGGAAGAG GAGTTTTGCG AAGCTTTTTG TGAACTTTGC 
ACCAGGCCGG AAGTGTATTT CTTACTTGTG CAGATATCAA AAAACAAAGA ATATTTGGAT 
GCCAATGATC TTATGCTTTT TTTGGAAGCT GAGCAAGGAG TCACCCATAT CACTGAGGAC 
ATGTGCTTAG ACATTATTCG GAGATATGAA CTTTCCGAAG AAGGACGTCA GAAAGGGTTT 
CTTGCAATTG ATGGCTTTAC CCAGTATTTG TTGTCACCAG AATGTAACAT TTTTGATCCT 
GAACAAAATA AGGTTGTCCA AGACATGACC CAGCCTTTAT CTCACTATTA CATCAATGCC 
TCTCACAACA CCTATCTCAT TGAAGACCAG TTCAGAGGAC CAGCTGATAT TAATGGGTAT 
GTCAGAGCTT TGAAAATGGG CTGTCGCAGC ATTGAACTTG ATGTAAATGA TGGCTCAGAC 
AACGAACCGA TCCTTTGTAA TCGAAACAAC ATGACAACAC ACCTTTCCTT TCGAAGTGTC 
ATAGAGGTGA TAAATAAATT TGCCTTTGTG GCTTCTGAAT ACCCACTCAT TCTTTGTTTG 
GGGAATCACT GCTCCCTACC ACAGCAGAAG GTAATGGTCC AACAGATGAA AAAGGTCTTT 
GGCAGTAAAC TCTATACTGA AGCACCTTTG CTGTCAGAAT CCTACCTCCC ATCACCAGAA 
AAATTAAAAA GAATGATCAT TGTGAAAGGA AAGAAATTGC CTTCTGATAC AGATATTTTA 
GAAGGAGAAG TTACAGATGA AGATGAAGAA GCCGAAATGT CTCGAAGGAT GTCAGTAGAT 
TACAATGGTG AGCAGAAACA GATCTTGCTG TGTAGGGAGC TCTCTGACTT GGTATCTATC 
TGTAAATCTG TTCAGTACAG GGATTTTGAA TTATCTATGA AAAGCCAAAA CTATTGGGAA 
ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC CGAATTGCAA ATGAATACCC AGAGGATTTT 
GTAAATTATA ATAAGAAGTT CTTATCACGA ATCTATCCAA GCGCCATGAG AATTGATTCC 
AGTAACCTGA ATCCACAGGA CTTTTGGAAT TGTGGCTGTC AGATTGTGGC AATGAATTTT 
CAGACTCCAG GTCCAATGAT GGACCTCCAC ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT 
GGTTATGTTC TAAGGCCGTC TATCATGCGA GATGAAGTTT CTTACTTCAG TGCAAACACA 
AAGGGCATTG TACCTGGGGT GTCTCCTCTA GTGCTTCATA TTAAGATTAT CAGTGGTCAG 
AACTTCCCAA AGCCCAAGGG AGCTTGTGCC AAAGGGGATG TCATAGATCC CTATGTGTGT 
ATAGAGATAC ATGGAATTCC AGCGGACTGT TCAGAACAAA GAACTAAAAC TGTACAACAA 
AACAGTGATA ATCCTATTTT TGATGAGACA TTTGAGTTTC AAGTGAACCT GCCTGAACTG 
GCCATGATCC GTTTTGTGGT TCTTGATGAT GACTACATTG GGGATGAGTT TATAGGGCAA 
TATACAATAC CGTTTGAATG CTTGCAGCCT GGATATCGGC ATGTTCCCCT CCGTTCCTTT 
GTGGGTGACA TCATGGAGCA TGTAACTCTT TTTGTCCACA TAGCAATAAC TAATAGAAGT 
GGAGGAGGAA AGCCACAGAA GCGCAGCCTT TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG 
TGTACCATGC TCAGGAATAT TGGTCTTAAA AACATAGATG ACATCTTTAA GATAGCTGTT 
CATCCCTTAC GAGAAGCCAT AGATATGAGA GAAAATATGC AGAATGCCAT AGTGTCTGTT 
AAGGAATTGT GTGGACTTCC TCCAATTGCC AGTCTGAAGC AGTGCCTGTT GACCCTGTCT 
TCTCGGCTCA TCACCAGTGA CAATACTCCC TCCGTCTCTC TTGTGATGAA AGACAACTTT 
CCTTATCTAG AGCCTCTGGG GGCAATTCCA GATATGCAGA AAAAGATGCT AGCTGCTTAT 
GATCTGATGA TCCAAGAGAG CCGATTTCTC ATAGAAATGG CAGACACAGT CCAGGAAAAG 
ATTGTGCAGT GTCAAAAAGC AGGCATGGAG TTCCATGAGG AGCTTCATAA TCTGGGGGCA 
AAAGAAGGCT TGAAAGGGAG AAAACTCAAC AAAGCAACTG AGAGCTTTGC TTGGAACATT 
ACAGTATTGA AGGGCCAGGG AGACCTGTTG AAGAACGCCA AGAATGAAGC CATAGAAAAC 
ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT 
GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG GCCATAGAGG AGAAGGAGAG CTGTGAGGAC 
AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025133728.1
CDS	546..3800
Translation	MSPPGTVWRRPLGAGGGTVAVGSPCPELRGPRRTVRPPSWRLLGRPLGAAASSRRILYQLSYQGSPKNKDPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_025277943.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025277943.1

ORF Insert Sequence:

ATGTCACCTC CGGGGACTGT GTGGCGGCGG CCCCTGGGGG CCGGTGGAGG GACCGTCGCA 
GTGGGGTCGC CCTGCCCGGA GCTGCGGGGC CCCCGGCGGA CAGTGAGGCC GCCCTCCTGG 
AGGCTGCTCG GGCGACCCCT CGGCGCAGCA GCATCATCAA GGCGGATTCT TTACCAGCTG 
AGCTACCAGG GAAGTCCTAA AAATAAGGAT CCTGCAAACC AAAAATGTGG TGGAAGAAAG 
AAAACCGTGT CTTTCAGCAG CATGCCGTCG GAAAAGAAAA TCAGCAGCGC AAGCGACTGC 
ATCACTTTCA TGCAGGCTGG CTGTGAACTG AAGAAAGTGC GGCCGAATTC GCGCATTTAC 
AACAGGTTCT TCACTCTGGA CACAGACCTG CAGGCTCTTC GCTGGGAACC TTCCAAGAAA 
GACCTCGAGA AAGCTAAGCT TGATATTTCT GCCATAAAAG AGATCCGACT GGGGAAAAAC 
ACGGAAACAT TTAGAAACAA TGGCCTTGCT GACCAGATTT GTGAGGATTG TGCCTTTTCC 
ATACTCCACG GGGAAAACTA CGAGTCTCTG GACTTAGTGG CCAATTCAGC GGACGTAGCA 
AACATCTGGG TGTCAGGATT GCGGTATCTG GTTTCTCGAA GTAAGCAACC CCTCGATTTT 
ATGGAGGGCA ACCAGAACAC ACCAAGGTTC ATGTGGTTGA AGACAGTGTT TGAAGCAGCA 
GATATCGATG GGAATGGGAT TATGTTGGAA GACACCTCTG TAGAGTTAAT AAAACAACTC 
AACCCTACTC TGAAGGAGTC CAAGATTAGG CTAAAATTTA AAGAAATCCA GAAGAGCAAA 
GAAAAACTGA CAACTCGAGT GACGGAAGAG GAGTTTTGCG AAGCTTTTTG TGAACTTTGC 
ACCAGGCCGG AAGTGTATTT CTTACTTGTG CAGATATCAA AAAACAAAGA ATATTTGGAT 
GCCAATGATC TTATGCTTTT TTTGGAAGCT GAGCAAGGAG TCACCCATAT CACTGAGGAC 
ATGTGCTTAG ACATTATTCG GAGATATGAA CTTTCCGAAG AAGGACGTCA GAAAGGGTTT 
CTTGCAATTG ATGGCTTTAC CCAGTATTTG TTGTCACCAG AATGTAACAT TTTTGATCCT 
GAACAAAATA AGGTTGTCCA AGACATGACC CAGCCTTTAT CTCACTATTA CATCAATGCC 
TCTCACAACA CCTATCTCAT TGAAGACCAG TTCAGAGGAC CAGCTGATAT TAATGGGTAT 
GTCAGAGCTT TGAAAATGGG CTGTCGCAGC ATTGAACTTG ATGTAAATGA TGGCTCAGAC 
AACGAACCGA TCCTTTGTAA TCGAAACAAC ATGACAACAC ACCTTTCCTT TCGAAGTGTC 
ATAGAGGTGA TAAATAAATT TGCCTTTGTG GCTTCTGAAT ACCCACTCAT TCTTTGTTTG 
GGGAATCACT GCTCCCTACC ACAGCAGAAG GTAATGGTCC AACAGATGAA AAAGGTCTTT 
GGCAGTAAAC TCTATACTGA AGCACCTTTG CTGTCAGAAT CCTACCTCCC ATCACCAGAA 
AAATTAAAAA GAATGATCAT TGTGAAAGGA AAGAAATTGC CTTCTGATAC AGATATTTTA 
GAAGGAGAAG TTACAGATGA AGATGAAGAA GCCGAAATGT CTCGAAGGAT GTCAGTAGAT 
TACAATGGTG AGCAGAAACA GATCTTGCTG TGTAGGGAGC TCTCTGACTT GGTATCTATC 
TGTAAATCTG TTCAGTACAG GGATTTTGAA TTATCTATGA AAAGCCAAAA CTATTGGGAA 
ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC CGAATTGCAA ATGAATACCC AGAGGATTTT 
GTAAATTATA ATAAGAAGTT CTTATCACGA ATCTATCCAA GCGCCATGAG AATTGATTCC 
AGTAACCTGA ATCCACAGGA CTTTTGGAAT TGTGGCTGTC AGATTGTGGC AATGAATTTT 
CAGACTCCAG GTCCAATGAT GGACCTCCAC ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT 
GGTTATGTTC TAAGGCCGTC TATCATGCGA GATGAAGTTT CTTACTTCAG TGCAAACACA 
AAGGGCATTG TACCTGGGGT GTCTCCTCTA GTGCTTCATA TTAAGATTAT CAGTGGTCAG 
AACTTCCCAA AGCCCAAGGG AGCTTGTGCC AAAGGGGATG TCATAGATCC CTATGTGTGT 
ATAGAGATAC ATGGAATTCC AGCGGACTGT TCAGAACAAA GAACTAAAAC TGTACAACAA 
AACAGTGATA ATCCTATTTT TGATGAGACA TTTGAGTTTC AAGTGAACCT GCCTGAACTG 
GCCATGATCC GTTTTGTGGT TCTTGATGAT GACTACATTG GGGATGAGTT TATAGGGCAA 
TATACAATAC CGTTTGAATG CTTGCAGCCT GGATATCGGC ATGTTCCCCT CCGTTCCTTT 
GTGGGTGACA TCATGGAGCA TGTAACTCTT TTTGTCCACA TAGCAATAAC TAATAGAAGT 
GGAGGAGGAA AGCCACAGAA GCGCAGCCTT TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG 
TGTACCATGC TCAGGAATAT TGGTCTTAAA AACATAGATG ACATCTTTAA GATAGCTGTT 
CATCCCTTAC GAGAAGCCAT AGATATGAGA GAAAATATGC AGAATGCCAT AGTGTCTGTT 
AAGGAATTGT GTGGACTTCC TCCAATTGCC AGTCTGAAGC AGTGCCTGTT GACCCTGTCT 
TCTCGGCTCA TCACCAGTGA CAATACTCCC TCCGTCTCTC TTGTGATGAA AGACAACTTT 
CCTTATCTAG AGCCTCTGGG GGCAATTCCA GATATGCAGA AAAAGATGCT AGCTGCTTAT 
GATCTGATGA TCCAAGAGAG CCGATTTCTC ATAGAAATGG CAGACACAGT CCAGGAAAAG 
ATTGTGCAGT GTCAAAAAGC AGGCATGGAG TTCCATGAGG AGCTTCATAA TCTGGGGGCA 
AAAGAAGGCT TGAAAGGGAG AAAACTCAAC AAAGCAACTG AGAGCTTTGC TTGGAACATT 
ACAGTATTGA AGGGCCAGGG AGACCTGTTG AAGAACGCCA AGAATGAAGC CATAGAAAAC 
ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT 
GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG GCCATAGAGG AGAAGGAGAG CTGTGAGGAC 
AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_006078170.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006078232.1 Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X4, mRNA.

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CloneID	OBu55752
Clone ID Related Accession (Same CDS sequence)	XM_006078170.2
Accession Version	XM_006078170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	inactive phospholipase C-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006078170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGC TGACTAGCTC GCAACCCCTA CTGTCTACTC GGAAAAAAGT GATTGCAGAT 
CCTGCAAACC AAAAATGTGG TGGAAGAAAG AAAACCGTGT CTTTCAGCAG CATGCCGTCG 
GAAAAGAAAA TCAGCAGCGC AAGCGACTGC ATCACTTTCA TGCAGGCTGG CTGTGAACTG 
AAGAAAGTGC GGCCGAATTC GCGCATTTAC AACAGGTTCT TCACTCTGGA CACAGACCTG 
CAGGCTCTTC GCTGGGAACC TTCCAAGAAA GACCTCGAGA AAGCTAAGCT TGATATTTCT 
GCCATAAAAG AGATCCGACT GGGGAAAAAC ACGGAAACAT TTAGAAACAA TGGCCTTGCT 
GACCAGATTT GTGAGGATTG TGCCTTTTCC ATACTCCACG GGGAAAACTA CGAGTCTCTG 
GACTTAGTGG CCAATTCAGC GGACGTAGCA AACATCTGGG TGTCAGGATT GCGGTATCTG 
GTTTCTCGAA GTAAGCAACC CCTCGATTTT ATGGAGGGCA ACCAGAACAC ACCAAGGTTC 
ATGTGGTTGA AGACAGTGTT TGAAGCAGCA GATATCGATG GGAATGGGAT TATGTTGGAA 
GACACCTCTG TAGAGTTAAT AAAACAACTC AACCCTACTC TGAAGGAGTC CAAGATTAGG 
CTAAAATTTA AAGAAATCCA GAAGAGCAAA GAAAAACTGA CAACTCGAGT GACGGAAGAG 
GAGTTTTGCG AAGCTTTTTG TGAACTTTGC ACCAGGCCGG AAGTGTATTT CTTACTTGTG 
CAGATATCAA AAAACAAAGA ATATTTGGAT GCCAATGATC TTATGCTTTT TTTGGAAGCT 
GAGCAAGGAG TCACCCATAT CACTGAGGAC ATGTGCTTAG ACATTATTCG GAGATATGAA 
CTTTCCGAAG AAGGACGTCA GAAAGGGTTT CTTGCAATTG ATGGCTTTAC CCAGTATTTG 
TTGTCACCAG AATGTAACAT TTTTGATCCT GAACAAAATA AGGTTGTCCA AGACATGACC 
CAGCCTTTAT CTCACTATTA CATCAATGCC TCTCACAACA CCTATCTCAT TGAAGACCAG 
TTCAGAGGAC CAGCTGATAT TAATGGGTAT GTCAGAGCTT TGAAAATGGG CTGTCGCAGC 
ATTGAACTTG ATGTAAATGA TGGCTCAGAC AACGAACCGA TCCTTTGTAA TCGAAACAAC 
ATGACAACAC ACCTTTCCTT TCGAAGTGTC ATAGAGGTGA TAAATAAATT TGCCTTTGTG 
GCTTCTGAAT ACCCACTCAT TCTTTGTTTG GGGAATCACT GCTCCCTACC ACAGCAGAAG 
GTAATGGTCC AACAGATGAA AAAGGTCTTT GGCAGTAAAC TCTATACTGA AGCACCTTTG 
CTGTCAGAAT CCTACCTCCC ATCACCAGAA AAATTAAAAA GAATGATCAT TGTGAAAGGA 
AAGAAATTGC CTTCTGATAC AGATATTTTA GAAGGAGAAG TTACAGATGA AGATGAAGAA 
GCCGAAATGT CTCGAAGGAT GTCAGTAGAT TACAATGGTG AGCAGAAACA GATCTTGCTG 
TGTAGGGAGC TCTCTGACTT GGTATCTATC TGTAAATCTG TTCAGTACAG GGATTTTGAA 
TTATCTATGA AAAGCCAAAA CTATTGGGAA ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC 
CGAATTGCAA ATGAATACCC AGAGGATTTT GTAAATTATA ATAAGAAGTT CTTATCACGA 
ATCTATCCAA GCGCCATGAG AATTGATTCC AGTAACCTGA ATCCACAGGA CTTTTGGAAT 
TGTGGCTGTC AGATTGTGGC AATGAATTTT CAGACTCCAG GTCCAATGAT GGACCTCCAC 
ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT GGTTATGTTC TAAGGCCGTC TATCATGCGA 
GATGAAGTTT CTTACTTCAG TGCAAACACA AAGGGCATTG TACCTGGGGT GTCTCCTCTA 
GTGCTTCATA TTAAGATTAT CAGTGGTCAG AACTTCCCAA AGCCCAAGGG AGCTTGTGCC 
AAAGGGGATG TCATAGATCC CTATGTGTGT ATAGAGATAC ATGGAATTCC AGCGGACTGT 
TCAGAACAAA GAACTAAAAC TGTACAACAA AACAGTGATA ATCCTATTTT TGATGAGACA 
TTTGAGTTTC AAGTGAACCT GCCTGAACTG GCCATGATCC GTTTTGTGGT TCTTGATGAT 
GACTACATTG GGGATGAGTT TATAGGGCAA TATACAATAC CGTTTGAATG CTTGCAGCCT 
GGATATCGGC ATGTTCCCCT CCGTTCCTTT GTGGGTGACA TCATGGAGCA TGTAACTCTT 
TTTGTCCACA TAGCAATAAC TAATAGAAGT GGAGGAGGAA AGCCACAGAA GCGCAGCCTT 
TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG TGTACCATGC TCAGGAATAT TGGTCTTAAA 
AACATAGATG ACATCTTTAA GATAGCTGTT CATCCCTTAC GAGAAGCCAT AGATATGAGA 
GAAAATATGC AGAATGCCAT AGTGTCTGTT AAGGAATTGT GTGGACTTCC TCCAATTGCC 
AGTCTGAAGC AGTGCCTGTT GACCCTGTCT TCTCGGCTCA TCACCAGTGA CAATACTCCC 
TCCGTCTCTC TTGTGATGAA AGACAACTTT CCTTATCTAG AGCCTCTGGG GGCAATTCCA 
GATATGCAGA AAAAGATGCT AGCTGCTTAT GATCTGATGA TCCAAGAGAG CCGATTTCTC 
ATAGAAATGG CAGACACAGT CCAGGAAAAG ATTGTGCAGT GTCAAAAAGC AGGCATGGAG 
TTCCATGAGG AGCTTCATAA TCTGGGGGCA AAAGAAGGCT TGAAAGGGAG AAAACTCAAC 
AAAGCAACTG AGAGCTTTGC TTGGAACATT ACAGTATTGA AGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006078232.1
CDS	516..3620
Translation	MERLTSSQPLLSTRKKVIADPANQKCGGRKKTVSFSSMPSEKKISSASDCITFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECNIFDPEQNKVVQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVNDGSDNEPILCNRNNMTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMVQQMKKVFGSKLYTEAPLLSESYLPSPEKLKRMIIVKGKKLPSDTDILEGEVTDEDEEAEMSRRMSVDYNGEQKQILLCRELSDLVSICKSVQYRDFELSMKSQNYWEICSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLVLHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKPQKRSLSVRMGKKVRECTMLRNIGLKNIDDIFKIAVHPLREAIDMRENMQNAIVSVKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDNFPYLEPLGAIPDMQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACSSCGLSKAPSSGAEVKSKRSLEAIEEKESCEDNGRL

Target ORF information:

RefSeq Version	XM_006078170.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis phospholipase C like 1 (inactive) (PLCL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006078170.2

ORF Insert Sequence:

ATGGAAAGGC TGACTAGCTC GCAACCCCTA CTGTCTACTC GGAAAAAAGT GATTGCAGAT 
CCTGCAAACC AAAAATGTGG TGGAAGAAAG AAAACCGTGT CTTTCAGCAG CATGCCGTCG 
GAAAAGAAAA TCAGCAGCGC AAGCGACTGC ATCACTTTCA TGCAGGCTGG CTGTGAACTG 
AAGAAAGTGC GGCCGAATTC GCGCATTTAC AACAGGTTCT TCACTCTGGA CACAGACCTG 
CAGGCTCTTC GCTGGGAACC TTCCAAGAAA GACCTCGAGA AAGCTAAGCT TGATATTTCT 
GCCATAAAAG AGATCCGACT GGGGAAAAAC ACGGAAACAT TTAGAAACAA TGGCCTTGCT 
GACCAGATTT GTGAGGATTG TGCCTTTTCC ATACTCCACG GGGAAAACTA CGAGTCTCTG 
GACTTAGTGG CCAATTCAGC GGACGTAGCA AACATCTGGG TGTCAGGATT GCGGTATCTG 
GTTTCTCGAA GTAAGCAACC CCTCGATTTT ATGGAGGGCA ACCAGAACAC ACCAAGGTTC 
ATGTGGTTGA AGACAGTGTT TGAAGCAGCA GATATCGATG GGAATGGGAT TATGTTGGAA 
GACACCTCTG TAGAGTTAAT AAAACAACTC AACCCTACTC TGAAGGAGTC CAAGATTAGG 
CTAAAATTTA AAGAAATCCA GAAGAGCAAA GAAAAACTGA CAACTCGAGT GACGGAAGAG 
GAGTTTTGCG AAGCTTTTTG TGAACTTTGC ACCAGGCCGG AAGTGTATTT CTTACTTGTG 
CAGATATCAA AAAACAAAGA ATATTTGGAT GCCAATGATC TTATGCTTTT TTTGGAAGCT 
GAGCAAGGAG TCACCCATAT CACTGAGGAC ATGTGCTTAG ACATTATTCG GAGATATGAA 
CTTTCCGAAG AAGGACGTCA GAAAGGGTTT CTTGCAATTG ATGGCTTTAC CCAGTATTTG 
TTGTCACCAG AATGTAACAT TTTTGATCCT GAACAAAATA AGGTTGTCCA AGACATGACC 
CAGCCTTTAT CTCACTATTA CATCAATGCC TCTCACAACA CCTATCTCAT TGAAGACCAG 
TTCAGAGGAC CAGCTGATAT TAATGGGTAT GTCAGAGCTT TGAAAATGGG CTGTCGCAGC 
ATTGAACTTG ATGTAAATGA TGGCTCAGAC AACGAACCGA TCCTTTGTAA TCGAAACAAC 
ATGACAACAC ACCTTTCCTT TCGAAGTGTC ATAGAGGTGA TAAATAAATT TGCCTTTGTG 
GCTTCTGAAT ACCCACTCAT TCTTTGTTTG GGGAATCACT GCTCCCTACC ACAGCAGAAG 
GTAATGGTCC AACAGATGAA AAAGGTCTTT GGCAGTAAAC TCTATACTGA AGCACCTTTG 
CTGTCAGAAT CCTACCTCCC ATCACCAGAA AAATTAAAAA GAATGATCAT TGTGAAAGGA 
AAGAAATTGC CTTCTGATAC AGATATTTTA GAAGGAGAAG TTACAGATGA AGATGAAGAA 
GCCGAAATGT CTCGAAGGAT GTCAGTAGAT TACAATGGTG AGCAGAAACA GATCTTGCTG 
TGTAGGGAGC TCTCTGACTT GGTATCTATC TGTAAATCTG TTCAGTACAG GGATTTTGAA 
TTATCTATGA AAAGCCAAAA CTATTGGGAA ATTTGTTCAT TTAGTGAAAC AGAGGCCAGC 
CGAATTGCAA ATGAATACCC AGAGGATTTT GTAAATTATA ATAAGAAGTT CTTATCACGA 
ATCTATCCAA GCGCCATGAG AATTGATTCC AGTAACCTGA ATCCACAGGA CTTTTGGAAT 
TGTGGCTGTC AGATTGTGGC AATGAATTTT CAGACTCCAG GTCCAATGAT GGACCTCCAC 
ACAGGCTGGT TTCTTCAGAA TGGAGGCTGT GGTTATGTTC TAAGGCCGTC TATCATGCGA 
GATGAAGTTT CTTACTTCAG TGCAAACACA AAGGGCATTG TACCTGGGGT GTCTCCTCTA 
GTGCTTCATA TTAAGATTAT CAGTGGTCAG AACTTCCCAA AGCCCAAGGG AGCTTGTGCC 
AAAGGGGATG TCATAGATCC CTATGTGTGT ATAGAGATAC ATGGAATTCC AGCGGACTGT 
TCAGAACAAA GAACTAAAAC TGTACAACAA AACAGTGATA ATCCTATTTT TGATGAGACA 
TTTGAGTTTC AAGTGAACCT GCCTGAACTG GCCATGATCC GTTTTGTGGT TCTTGATGAT 
GACTACATTG GGGATGAGTT TATAGGGCAA TATACAATAC CGTTTGAATG CTTGCAGCCT 
GGATATCGGC ATGTTCCCCT CCGTTCCTTT GTGGGTGACA TCATGGAGCA TGTAACTCTT 
TTTGTCCACA TAGCAATAAC TAATAGAAGT GGAGGAGGAA AGCCACAGAA GCGCAGCCTT 
TCAGTGAGAA TGGGGAAGAA AGTTCGGGAG TGTACCATGC TCAGGAATAT TGGTCTTAAA 
AACATAGATG ACATCTTTAA GATAGCTGTT CATCCCTTAC GAGAAGCCAT AGATATGAGA 
GAAAATATGC AGAATGCCAT AGTGTCTGTT AAGGAATTGT GTGGACTTCC TCCAATTGCC 
AGTCTGAAGC AGTGCCTGTT GACCCTGTCT TCTCGGCTCA TCACCAGTGA CAATACTCCC 
TCCGTCTCTC TTGTGATGAA AGACAACTTT CCTTATCTAG AGCCTCTGGG GGCAATTCCA 
GATATGCAGA AAAAGATGCT AGCTGCTTAT GATCTGATGA TCCAAGAGAG CCGATTTCTC 
ATAGAAATGG CAGACACAGT CCAGGAAAAG ATTGTGCAGT GTCAAAAAGC AGGCATGGAG 
TTCCATGAGG AGCTTCATAA TCTGGGGGCA AAAGAAGGCT TGAAAGGGAG AAAACTCAAC 
AAAGCAACTG AGAGCTTTGC TTGGAACATT ACAGTATTGA AGGGCCAGGG AGACCTGTTG 
AAGAACGCCA AGAATGAAGC CATAGAAAAC ATGAAGCAGA TCCAGCTGGC GTGCTCGTCT 
TGCGGCCTCA GTAAAGCTCC CAGCAGTGGT GCTGAGGTGA AGAGTAAGCG CAGCCTGGAG 
GCCATAGAGG AGAAGGAGAG CTGTGAGGAC AATGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PLCL1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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