AFAP1L2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFAP1L2
Entrez Gene ID	102404932
Full Name	actin filament associated protein 1 like 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006076962.1	XP_006077024.1	actin filament-associated protein 1-like 2
XM_025274269.1	XP_025130054.1	LOW QUALITY PROTEIN: actin filament-associated protein 1-like 2

Pathways from BioSystems
Gene Ontology

The following AFAP1L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu30411	XM_006076962.1 Latest version!	Bubalus bubalis actin filament associated protein 1-like 2 (AFAP1L2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu59284	XM_025274269.1 Latest version!	Bubalus bubalis actin filament associated protein 1 like 2 (AFAP1L2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFAP1L2 ( XM_006076962.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006077024.1 Bubalus bubalis actin filament associated protein 1-like 2 (AFAP1L2), mRNA.

Price & Availability↑

CloneID	OBu30411
Clone ID Related Accession (Same CDS sequence)	XM_006076962.1
Accession Version	XM_006076962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	actin filament-associated protein 1-like 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCAA AGCCAGATTC CAGACGTGGA GACTGCAGGC CCACAGTGGC CTCCCTTGGC 
TTTCTCGACT TGAGCCTCTT TCTGTCTGTT CCAGCACAGG GCTGTTGCTG TCTGCTGCTG 
CAGAGAAGGA TGCTGCAGGT TTCTGCTTCC CTGGAGCAGC TGCTGACAGA GCTGGATGAC 
TTCCTCAGGA TTCTTGACCA GGAGAACCTG AGCAGCACAG CCGTGGTGAA GAAGAGTGGC 
CTGGCCGAGC TTCTCCGCTT ATACACCAAG AGCAACAGTT CTGATGAGGA ATACATTTAT 
ATGAACAAAG TGACAGTCCA CAAGCAACAG AATGCCGAGT CCCAAGACAA AGCCCCCGAG 
CAGCAGAACC CGCTGACCAA CGGTGAGCCC CCCCAGCCCT CCTCGGCCCC TCAGAAGAGT 
CTCCCAGACC TCCCACCCCC CAAGATGATT CCAGAACGTA AACAGCTCTC TGTCCCGAAG 
ATCGAGTCTC CAGAGGGTTA CTATGAAGAG GCTGAGCCGT ATGACACATC CCTCAATGAG 
GACGGAGAGG CCGTGAGCAG CTCCTACGAG TCCTACGACG AGGAGGAGAG CAGCAAGGGC 
AAGTCGGCCC CCTACCAGTG GCCCTCGCCC GAGGCCAGCA TCGAGCTGAT GCGCGACGCC 
CGCATCTGCG CCTTCCTCTG GCGCAAGAAG TGGCTGGGCC AGTGGGCCAA GCAGCTGTGC 
GTCATAAAGG ACACCAGGCT CCTGTGTTAC AAATCCTCCA AGGACCACAG TCCTCAGCTG 
GACGTGAGCT TGCTGGGCAG CAGCGTGGTG CACAAGGAGA AGCAAGTGCG GAAGAAGGAG 
CACAAGCTGA AGATCACGCC GCTGAACGCC GACGTGATCG TGCTGGGCCT GCAGAGCAGG 
GACCAGGCGG AGCAGTGGCT CAGGGTCATT CAGGAGGTCA GCGGCCTGCC TTCTGAAGGA 
GCTTGTGAGG GAAGCCAGTT CACCCCAGAT GCCCAGCGCC TGAGCTGTCC AAAGCCAGAT 
ATAACGGAGA AGTACCTGTC AGCCTCGGAG TGTGGAAGCC CCATAGATGG TCACCCTGAA 
GTCCCAGAGA CCAAAGACGT CAAGAAGAAA TGCTCTGCTG GTCTCAAACT GAGCAACCTG 
ATGAACCTGG GCAGGAAGAA ATCTACCTCG CTGGAGCCTC CGGACAGGTC CCTTGAGACA 
TCCAGCTACC TGAACGTGCT GGTCAACAGC CAATGGAAGT CACGCTGGTG CTCCGTCAGG 
GACAGTCACC TGTACTTCTA CCAGGACCGG AACCGGAGCA AGGCGGCCCA GCAGCCCCTC 
AGCCTTCTGG GCTGTGAGGT GGTGCCGGAC CCGAGCCCGG ACCACCTCTA CTCCTTCCGC 
ATCCTCCACA ATGGCGAGGA GCTGGCCAAG CTCGAGGCCA AGTCTTCCGA GGAAATGGGC 
CACTGGCTAG GCCTCCTGCT CTCTGAGTCG GGCTCCAAGA CAGACCCGGA AGAATTCACC 
TATGACTATG TGGATGCCGA TAGAGTTTCC TGTATCGTGA GCGCTGCCAA AACCTCTCTG 
CTGCTGATGC AGAGAAAGTT CTCGGAGCCG AACACTTACA TTGACGGCCT GCCCAGCCAG 
GATCGCCAGG AGCTGCTGTA CGACGATGTG GAGGTGTCAG AGCTGACAAC CGCGGGGGAA 
GCCCCCGAGG AAGCCACCCC TGCAACAGAT GCTCCAGGTG AGCCTGACCC TGACCGTGTG 
TACCTGGACC TCACGCCCAT CAAGTCCTTC CTACACGGTG ACAGCGGAGC CCGGGCCCCA 
TCACCCACGC CGCCCCACCA GGACCCTCCG GCCGAGACTC TCCCTCTCCC TGAAGACTCG 
GACCCCGCCC CTGATGAGCC CCTCATAAAG TCTCCAGAGA ACCCCGAGTT GCAGATGCAG 
CAGGAGAGTC AGGAACCTGA GGAGCCCTCC CTGGGAGGCA CAGAGGTCAA GCTCCAGGCT 
GGACAGCAGA AGACCTCCCC TTCCCCGAGC TGCCCTGACA CGGTGGCTGT CACCCCGGCT 
GGGTCCAGCC CACCTGTGAA GGACAGGATG AAGGCAGCCA GCCCAGAGAT CAAGCTCGGC 
AAGAACCGGA CAGAAGCCGA GGTGAAGCGG TACACGGAGG AGAAGGAGAG GCTTGAGAAG 
AAGAAGGAAG AGATCCGAGG GCACCTGGCT CAGCTCCGGA GGGAGAAACG GGAGCTGAAG 
GAGACCCTGT TAAAGTGTAC AGACAAGGGG GCGGCGGCCA GCCTGGAGCA GAAGCTGAAG 
GAGGTGGATG AGGAGTGCAG GGTGGAGGAG CGCAGGCGTG TGGACCTAGA GCTCAGCATC 
GTGGAGGTGA AGGACAGCCT GAGGAAGGCG GAGGCCGGGC CCGTGACACT GGGCACCACC 
GTGGACACCA CCCACCTGGA GAGCGTGAGC CCCCGGCCCA AAGCGGCCAC CCCGATTCCT 
GCCCCCGACT GTACCCCGGT CAACTCCGCA ACTGCACTCA AGAACAGGCC TCTGTCGGTT 
ATGGTCACGG GCAAAGGCAC CGTCCTCCAG AAAGCCAAGG AATGGGAGAA GAAAGGAGCA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006077024.1
CDS	1..2586
Translation	MISKPDSRRGDCRPTVASLGFLDLSLFLSVPAQGCCCLLLQRRMLQVSASLEQLLTELDDFLRILDQENLSSTAVVKKSGLAELLRLYTKSNSSDEEYIYMNKVTVHKQQNAESQDKAPEQQNPLTNGEPPQPSSAPQKSLPDLPPPKMIPERKQLSVPKIESPEGYYEEAEPYDTSLNEDGEAVSSSYESYDEEESSKGKSAPYQWPSPEASIELMRDARICAFLWRKKWLGQWAKQLCVIKDTRLLCYKSSKDHSPQLDVSLLGSSVVHKEKQVRKKEHKLKITPLNADVIVLGLQSRDQAEQWLRVIQEVSGLPSEGACEGSQFTPDAQRLSCPKPDITEKYLSASECGSPIDGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEPPDRSLETSSYLNVLVNSQWKSRWCSVRDSHLYFYQDRNRSKAAQQPLSLLGCEVVPDPSPDHLYSFRILHNGEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIVSAAKTSLLLMQRKFSEPNTYIDGLPSQDRQELLYDDVEVSELTTAGEAPEEATPATDAPGEPDPDRVYLDLTPIKSFLHGDSGARAPSPTPPHQDPPAETLPLPEDSDPAPDEPLIKSPENPELQMQQESQEPEEPSLGGTEVKLQAGQQKTSPSPSCPDTVAVTPAGSSPPVKDRMKAASPEIKLGKNRTEAEVKRYTEEKERLEKKKEEIRGHLAQLRREKRELKETLLKCTDKGAAASLEQKLKEVDEECRVEERRRVDLELSIVEVKDSLRKAEAGPVTLGTTVDTTHLESVSPRPKAATPIPAPDCTPVNSATALKNRPLSVMVTGKGTVLQKAKEWEKKGAS

Target ORF information:

RefSeq Version	XM_006076962.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actin filament associated protein 1-like 2 (AFAP1L2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076962.1

ORF Insert Sequence:

ATGATCTCAA AGCCAGATTC CAGACGTGGA GACTGCAGGC CCACAGTGGC CTCCCTTGGC 
TTTCTCGACT TGAGCCTCTT TCTGTCTGTT CCAGCACAGG GCTGTTGCTG TCTGCTGCTG 
CAGAGAAGGA TGCTGCAGGT TTCTGCTTCC CTGGAGCAGC TGCTGACAGA GCTGGATGAC 
TTCCTCAGGA TTCTTGACCA GGAGAACCTG AGCAGCACAG CCGTGGTGAA GAAGAGTGGC 
CTGGCCGAGC TTCTCCGCTT ATACACCAAG AGCAACAGTT CTGATGAGGA ATACATTTAT 
ATGAACAAAG TGACAGTCCA CAAGCAACAG AATGCCGAGT CCCAAGACAA AGCCCCCGAG 
CAGCAGAACC CGCTGACCAA CGGTGAGCCC CCCCAGCCCT CCTCGGCCCC TCAGAAGAGT 
CTCCCAGACC TCCCACCCCC CAAGATGATT CCAGAACGTA AACAGCTCTC TGTCCCGAAG 
ATCGAGTCTC CAGAGGGTTA CTATGAAGAG GCTGAGCCGT ATGACACATC CCTCAATGAG 
GACGGAGAGG CCGTGAGCAG CTCCTACGAG TCCTACGACG AGGAGGAGAG CAGCAAGGGC 
AAGTCGGCCC CCTACCAGTG GCCCTCGCCC GAGGCCAGCA TCGAGCTGAT GCGCGACGCC 
CGCATCTGCG CCTTCCTCTG GCGCAAGAAG TGGCTGGGCC AGTGGGCCAA GCAGCTGTGC 
GTCATAAAGG ACACCAGGCT CCTGTGTTAC AAATCCTCCA AGGACCACAG TCCTCAGCTG 
GACGTGAGCT TGCTGGGCAG CAGCGTGGTG CACAAGGAGA AGCAAGTGCG GAAGAAGGAG 
CACAAGCTGA AGATCACGCC GCTGAACGCC GACGTGATCG TGCTGGGCCT GCAGAGCAGG 
GACCAGGCGG AGCAGTGGCT CAGGGTCATT CAGGAGGTCA GCGGCCTGCC TTCTGAAGGA 
GCTTGTGAGG GAAGCCAGTT CACCCCAGAT GCCCAGCGCC TGAGCTGTCC AAAGCCAGAT 
ATAACGGAGA AGTACCTGTC AGCCTCGGAG TGTGGAAGCC CCATAGATGG TCACCCTGAA 
GTCCCAGAGA CCAAAGACGT CAAGAAGAAA TGCTCTGCTG GTCTCAAACT GAGCAACCTG 
ATGAACCTGG GCAGGAAGAA ATCTACCTCG CTGGAGCCTC CGGACAGGTC CCTTGAGACA 
TCCAGCTACC TGAACGTGCT GGTCAACAGC CAATGGAAGT CACGCTGGTG CTCCGTCAGG 
GACAGTCACC TGTACTTCTA CCAGGACCGG AACCGGAGCA AGGCGGCCCA GCAGCCCCTC 
AGCCTTCTGG GCTGTGAGGT GGTGCCGGAC CCGAGCCCGG ACCACCTCTA CTCCTTCCGC 
ATCCTCCACA ATGGCGAGGA GCTGGCCAAG CTCGAGGCCA AGTCTTCCGA GGAAATGGGC 
CACTGGCTAG GCCTCCTGCT CTCTGAGTCG GGCTCCAAGA CAGACCCGGA AGAATTCACC 
TATGACTATG TGGATGCCGA TAGAGTTTCC TGTATCGTGA GCGCTGCCAA AACCTCTCTG 
CTGCTGATGC AGAGAAAGTT CTCGGAGCCG AACACTTACA TTGACGGCCT GCCCAGCCAG 
GATCGCCAGG AGCTGCTGTA CGACGATGTG GAGGTGTCAG AGCTGACAAC CGCGGGGGAA 
GCCCCCGAGG AAGCCACCCC TGCAACAGAT GCTCCAGGTG AGCCTGACCC TGACCGTGTG 
TACCTGGACC TCACGCCCAT CAAGTCCTTC CTACACGGTG ACAGCGGAGC CCGGGCCCCA 
TCACCCACGC CGCCCCACCA GGACCCTCCG GCCGAGACTC TCCCTCTCCC TGAAGACTCG 
GACCCCGCCC CTGATGAGCC CCTCATAAAG TCTCCAGAGA ACCCCGAGTT GCAGATGCAG 
CAGGAGAGTC AGGAACCTGA GGAGCCCTCC CTGGGAGGCA CAGAGGTCAA GCTCCAGGCT 
GGACAGCAGA AGACCTCCCC TTCCCCGAGC TGCCCTGACA CGGTGGCTGT CACCCCGGCT 
GGGTCCAGCC CACCTGTGAA GGACAGGATG AAGGCAGCCA GCCCAGAGAT CAAGCTCGGC 
AAGAACCGGA CAGAAGCCGA GGTGAAGCGG TACACGGAGG AGAAGGAGAG GCTTGAGAAG 
AAGAAGGAAG AGATCCGAGG GCACCTGGCT CAGCTCCGGA GGGAGAAACG GGAGCTGAAG 
GAGACCCTGT TAAAGTGTAC AGACAAGGGG GCGGCGGCCA GCCTGGAGCA GAAGCTGAAG 
GAGGTGGATG AGGAGTGCAG GGTGGAGGAG CGCAGGCGTG TGGACCTAGA GCTCAGCATC 
GTGGAGGTGA AGGACAGCCT GAGGAAGGCG GAGGCCGGGC CCGTGACACT GGGCACCACC 
GTGGACACCA CCCACCTGGA GAGCGTGAGC CCCCGGCCCA AAGCGGCCAC CCCGATTCCT 
GCCCCCGACT GTACCCCGGT CAACTCCGCA ACTGCACTCA AGAACAGGCC TCTGTCGGTT 
ATGGTCACGG GCAAAGGCAC CGTCCTCCAG AAAGCCAAGG AATGGGAGAA GAAAGGAGCA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFAP1L2 ( XM_025274269.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130054.1 Bubalus bubalis actin filament associated protein 1 like 2 (AFAP1L2), mRNA.

Price & Availability↑

CloneID	OBu59284
Clone ID Related Accession (Same CDS sequence)	XM_025274269.1
Accession Version	XM_025274269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: actin filament-associated protein 1-like 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037567.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGCGGT TCAAAGCACA GGGCTGTTGC TGTCTGCTGC TGCAGAGAAG GATGCTGCAG 
GTTTCTGCTT CCCTGGAGCA GCTGCTGACA GAGCTGGATG ACTTCCTCAG GATTCTTGAC 
CAGGAGAACC TGAGCAGCAC AGCCGTGGTG AAGAAGAGTG GCCTGGCCGA GCTTCTCCGC 
TTATACACCA AGAGCAACAG TTCTGATGAG GAATACATTT ATATGAACAA AGTGACAGTC 
CACAAGCAAC AGAATGCCGA GTCCCAAGAC AAAGCCCCCG AGCAGCAGAA CCCGCTGACC 
AACGGTGAGC CCCCCCAGCC CTCCTCGGCC CCTCAGAAGA GTCTCCCAGA CCTCCCACCC 
CCCAAGATGA TTCCAGAACG TAAACAGCTC TCTGTCCCGA AGATCGAGTC TCCAGAGGGT 
TACTATGAAG AGGCTGAGCC GTATGACACA TCCCTCAATG GCCATCCCAG CAGATATCCC 
CCCTGTGGAG TCCCCAGATG GGTGCAGGTG CCTGAAGGAG TTGTTTACGC CACCATCACC 
TTGGAGGACG GAGAGGCCGT GAGCAGCTCC TACGAGTCCT ACGACGAGGA GGAGAGCAGC 
AAGGGCAAGT CGGCCCCCTA CCAGTGGCCC TCGCCCGAGG CCAGCATCGA GCTGATGCGC 
GACGCCCGCA TCTGCGCCTT CCTCTGGCGC AAGAAGTGGC TGGGCCAGTG GGCCAAGCAG 
CTGTGCGTCA TAAAGGACAC CAGGCTCCTG TGTTACAAAT CCTCCAAGGA CCACAGTCCT 
CAGCTGGACG TGAGCTTGCT GGGCAGCAGC GTGGTGCACA AGGAGAAGCA AGTGCGGAAG 
AAGGAGCACA AGCTGAAGAT CACGCCGCTG AACGCCGACG TGATCGTGCT GGGCCTGCAG 
AGCAGGGACC AGGCGGAGCA GTGGCTCAGG GTCATTCAGG AGGTCAGCGG CCTGCCTTCC 
GAAGGAGCTT GTGAGGGAAG CCAGTTCACC CCAGATGCCC AGCGCCTGAG CTGTCCAAAG 
CCAGATATAA CGGAGAAGTA CCTGTCAGCC TCGGAGTGTG GAAGCCCCAT AGATGGTCAC 
CCTGAAGTCC CAGAGACCAA AGACGTCAAG AAGAAATGCT CTGCTGGTCT CAAACTGAGC 
AACCTGATGA ACCTGGGCAG GAAGAAATCT ACCTCGCTGG AGCCTCCGGA CAGGTCCCTT 
GAGACATCCA GCTACCTGAA CGTGCTGGTC AACAGCCAAT GGAAGTCACG CTGGTGCTCC 
GTCAGGGACA GTCACCTGTA CTTCTACCAG GACCGGAACC GGAGCAAGGC GGCCCAGCAG 
CCCCTCAGCC TTCTGGGCTG TGAGGTGGTG CCGGACCCGA GCCCGGACCA CCTCTACTCC 
TTCCGCATCC TCCACAATGG CGAGGAGCTG GCCAAGCTCG AGGCCAAGTC TTCCGAGGAA 
ATGGGCCACT GGCTAGGCCT CCTGCTCTCT GAGTCGGGCT CCAAGACAGA CCCGGAAGAA 
TTCACCTATG ACTATGTGGA TGCCGATAGA GTTTCCTGTA TCGTGAGCGC TGCCAAAACC 
TCTCTGCTGC TGATGCAGAG AAAGTTCTCG GAGCCGAACA CTTACATTGA CGGCCTGCCC 
AGCCAGGATC GCCAGGAGCT GCTGTACGAC GATGTGGAGG TGTCAGAGCT GACAACCGCG 
GGGGAAGCCC CCGAGGAAGC CACCCCTGCA ACAGATGCTC CAGGTGAGCC TGACCCTGAC 
CGTGTGTACC TGGACCTCAC GCCCATCAAG TCCTTCCTAC ACGGTGACAG CGGAGCCCGG 
GCCCCATCAC CCACGCCGCC CCACCAGGAC CCTCCGGCCG AGACTCTCCC TCTCCCTGAA 
GACTCGGACC CCGCCCCTGA TGAGCCCCTC ATAAAGTCTC CAGAGAACCC CGAGTTGCAG 
ATGCAGCAGG AGAGTCAGGA ACCTGAGGAG CCCTCCCTGG GAGGCACAGA GGTCAAGCTC 
CAGGCTGGAC AGCAGAAGAC CTCCCCTTCC CCGAGCTGCC CTGACACGGT GGCTGTCACC 
CCGGCTGGGT CCAGCCCACC TGTGAAGGAC AGGATGAAGG CAGCCAGCCC AGAGATCAAG 
CTCGGCAAGA ACCGGACAGA ANGCGAGGTG AAGCGGTACA CGGAGGAGAA GGAGAGGCTT 
GAGAAGAAGA AGGAAGAGAT CCGAGGGCAC CTGGCTCAGC TCCGGAGGGA GAAACGGGAG 
CTGAAGGAGA CCCTGTTAAA GTGTACAGAC AAGGGGGCGG CGGCCAGCCT GGAGCAGAAG 
CTGAAGGAGG TGGATGAGGA GTGCAGGGTG GAGGAGCGCA GGCGTGTGGA CCTAGAGCTC 
AGCATCGTGG AGGTGAAGGA CAGCCTGAGG AAGGCGGAGG CCGGGCCCGT GACACTGGGC 
ACCACCGTGG ACACCACCCA CCTGGAGAGC GTGAGCCCCC GGCCCAAAGC GGCCACCCCG 
ATTCCTGCCC CCGACTGTAC CCCGGTCAAC TCCGCAACTG CACTCAAGAA CAGGCCTCTG 
TCGGTTATGG TCACGGGCAA AGGCACCGTC CTCCAGAAAG CCAAGGAATG GGAGAAGAAA 
GGAGCAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130054.1
CDS	29..2620
Translation	MERFKAQGCCCLLLQRRMLQVSASLEQLLTELDDFLRILDQENLSSTAVVKKSGLAELLRLYTKSNSSDEEYIYMNKVTVHKQQNAESQDKAPEQQNPLTNGEPPQPSSAPQKSLPDLPPPKMIPERKQLSVPKIESPEGYYEEAEPYDTSLNGHPSRYPPCGVPRWVQVPEGVVYATITLEDGEAVSSSYESYDEEESSKGKSAPYQWPSPEASIELMRDARICAFLWRKKWLGQWAKQLCVIKDTRLLCYKSSKDHSPQLDVSLLGSSVVHKEKQVRKKEHKLKITPLNADVIVLGLQSRDQAEQWLRVIQEVSGLPSEGACEGSQFTPDAQRLSCPKPDITEKYLSASECGSPIDGHPEVPETKDVKKKCSAGLKLSNLMNLGRKKSTSLEPPDRSLETSSYLNVLVNSQWKSRWCSVRDSHLYFYQDRNRSKAAQQPLSLLGCEVVPDPSPDHLYSFRILHNGEELAKLEAKSSEEMGHWLGLLLSESGSKTDPEEFTYDYVDADRVSCIVSAAKTSLLLMQRKFSEPNTYIDGLPSQDRQELLYDDVEVSELTTAGEAPEEATPATDAPGEPDPDRVYLDLTPIKSFLHGDSGARAPSPTPPHQDPPAETLPLPEDSDPAPDEPLIKSPENPELQMQQESQEPEEPSLGGTEVKLQAGQQKTSPSPSCPDTVAVTPAGSSPPVKDRMKAASPEIKLGKNRTEnullEVKRYTEEKERLEKKKEEIRGHLAQLRREKRELKETLLKCTDKGAAASLEQKLKEVDEECRVEERRRVDLELSIVEVKDSLRKAEAGPVTLGTTVDTTHLESVSPRPKAATPIPAPDCTPVNSATALKNRPLSVMVTGKGTVLQKAKEWEKKGAS

Target ORF information:

RefSeq Version	XM_025274269.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actin filament associated protein 1 like 2 (AFAP1L2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274269.1

ORF Insert Sequence:

ATGGAGCGGT TCAAAGCACA GGGCTGTTGC TGTCTGCTGC TGCAGAGAAG GATGCTGCAG 
GTTTCTGCTT CCCTGGAGCA GCTGCTGACA GAGCTGGATG ACTTCCTCAG GATTCTTGAC 
CAGGAGAACC TGAGCAGCAC AGCCGTGGTG AAGAAGAGTG GCCTGGCCGA GCTTCTCCGC 
TTATACACCA AGAGCAACAG TTCTGATGAG GAATACATTT ATATGAACAA AGTGACAGTC 
CACAAGCAAC AGAATGCCGA GTCCCAAGAC AAAGCCCCCG AGCAGCAGAA CCCGCTGACC 
AACGGTGAGC CCCCCCAGCC CTCCTCGGCC CCTCAGAAGA GTCTCCCAGA CCTCCCACCC 
CCCAAGATGA TTCCAGAACG TAAACAGCTC TCTGTCCCGA AGATCGAGTC TCCAGAGGGT 
TACTATGAAG AGGCTGAGCC GTATGACACA TCCCTCAATG GCCATCCCAG CAGATATCCC 
CCCTGTGGAG TCCCCAGATG GGTGCAGGTG CCTGAAGGAG TTGTTTACGC CACCATCACC 
TTGGAGGACG GAGAGGCCGT GAGCAGCTCC TACGAGTCCT ACGACGAGGA GGAGAGCAGC 
AAGGGCAAGT CGGCCCCCTA CCAGTGGCCC TCGCCCGAGG CCAGCATCGA GCTGATGCGC 
GACGCCCGCA TCTGCGCCTT CCTCTGGCGC AAGAAGTGGC TGGGCCAGTG GGCCAAGCAG 
CTGTGCGTCA TAAAGGACAC CAGGCTCCTG TGTTACAAAT CCTCCAAGGA CCACAGTCCT 
CAGCTGGACG TGAGCTTGCT GGGCAGCAGC GTGGTGCACA AGGAGAAGCA AGTGCGGAAG 
AAGGAGCACA AGCTGAAGAT CACGCCGCTG AACGCCGACG TGATCGTGCT GGGCCTGCAG 
AGCAGGGACC AGGCGGAGCA GTGGCTCAGG GTCATTCAGG AGGTCAGCGG CCTGCCTTCC 
GAAGGAGCTT GTGAGGGAAG CCAGTTCACC CCAGATGCCC AGCGCCTGAG CTGTCCAAAG 
CCAGATATAA CGGAGAAGTA CCTGTCAGCC TCGGAGTGTG GAAGCCCCAT AGATGGTCAC 
CCTGAAGTCC CAGAGACCAA AGACGTCAAG AAGAAATGCT CTGCTGGTCT CAAACTGAGC 
AACCTGATGA ACCTGGGCAG GAAGAAATCT ACCTCGCTGG AGCCTCCGGA CAGGTCCCTT 
GAGACATCCA GCTACCTGAA CGTGCTGGTC AACAGCCAAT GGAAGTCACG CTGGTGCTCC 
GTCAGGGACA GTCACCTGTA CTTCTACCAG GACCGGAACC GGAGCAAGGC GGCCCAGCAG 
CCCCTCAGCC TTCTGGGCTG TGAGGTGGTG CCGGACCCGA GCCCGGACCA CCTCTACTCC 
TTCCGCATCC TCCACAATGG CGAGGAGCTG GCCAAGCTCG AGGCCAAGTC TTCCGAGGAA 
ATGGGCCACT GGCTAGGCCT CCTGCTCTCT GAGTCGGGCT CCAAGACAGA CCCGGAAGAA 
TTCACCTATG ACTATGTGGA TGCCGATAGA GTTTCCTGTA TCGTGAGCGC TGCCAAAACC 
TCTCTGCTGC TGATGCAGAG AAAGTTCTCG GAGCCGAACA CTTACATTGA CGGCCTGCCC 
AGCCAGGATC GCCAGGAGCT GCTGTACGAC GATGTGGAGG TGTCAGAGCT GACAACCGCG 
GGGGAAGCCC CCGAGGAAGC CACCCCTGCA ACAGATGCTC CAGGTGAGCC TGACCCTGAC 
CGTGTGTACC TGGACCTCAC GCCCATCAAG TCCTTCCTAC ACGGTGACAG CGGAGCCCGG 
GCCCCATCAC CCACGCCGCC CCACCAGGAC CCTCCGGCCG AGACTCTCCC TCTCCCTGAA 
GACTCGGACC CCGCCCCTGA TGAGCCCCTC ATAAAGTCTC CAGAGAACCC CGAGTTGCAG 
ATGCAGCAGG AGAGTCAGGA ACCTGAGGAG CCCTCCCTGG GAGGCACAGA GGTCAAGCTC 
CAGGCTGGAC AGCAGAAGAC CTCCCCTTCC CCGAGCTGCC CTGACACGGT GGCTGTCACC 
CCGGCTGGGT CCAGCCCACC TGTGAAGGAC AGGATGAAGG CAGCCAGCCC AGAGATCAAG 
CTCGGCAAGA ACCGGACAGA ANGCGAGGTG AAGCGGTACA CGGAGGAGAA GGAGAGGCTT 
GAGAAGAAGA AGGAAGAGAT CCGAGGGCAC CTGGCTCAGC TCCGGAGGGA GAAACGGGAG 
CTGAAGGAGA CCCTGTTAAA GTGTACAGAC AAGGGGGCGG CGGCCAGCCT GGAGCAGAAG 
CTGAAGGAGG TGGATGAGGA GTGCAGGGTG GAGGAGCGCA GGCGTGTGGA CCTAGAGCTC 
AGCATCGTGG AGGTGAAGGA CAGCCTGAGG AAGGCGGAGG CCGGGCCCGT GACACTGGGC 
ACCACCGTGG ACACCACCCA CCTGGAGAGC GTGAGCCCCC GGCCCAAAGC GGCCACCCCG 
ATTCCTGCCC CCGACTGTAC CCCGGTCAAC TCCGCAACTG CACTCAAGAA CAGGCCTCTG 
TCGGTTATGG TCACGGGCAA AGGCACCGTC CTCCAGAAAG CCAAGGAATG GGAGAAGAAA 
GGAGCAAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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AFAP1L2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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