CASKIN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CASKIN1
Entrez Gene ID	102404184
Full Name	CASK interacting protein 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006076884.1	XP_006076946.1	caskin-1
XM_025274994.1	XP_025130779.1	caskin-1 isoform X1
XM_025274998.1	XP_025130783.1	caskin-1 isoform X4
XM_025274996.1	XP_025130781.1	caskin-1 isoform X2
XM_025274997.1	XP_025130782.1	caskin-1 isoform X3

Pathways from BioSystems
Gene Ontology

The following CASKIN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASKIN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu59863	XM_025274994.1 Latest version!	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu59864	XM_025274996.1 Latest version!	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu59865	XM_025274997.1 Latest version!	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu59866	XM_025274998.1 Latest version!	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu30348	XM_006076884.1 Latest version!	Bubalus bubalis CASK interacting protein 1 (CASKIN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CASKIN1 ( XM_025274994.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130779.1 Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu59863
Clone ID Related Accession (Same CDS sequence)	XM_025274994.1
Accession Version	XM_025274994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	caskin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGG TAGGTGGGGT GGCGGCAGGG 
CCAGGGTGCC TGGGTCTGGA GCAGCACTGG CCAGCCAGCC TGTCTGGGTT CCAGCTCCTG 
GCAACGGTGC TCTCCCAGAA GCCGGTGTCC GACTCCAGCC CCGGGGACAG CCCGGTCAAG 
CCTCCGGAGG GCCCTGCAGG TGCGACCCGG GCCCAGCCTC CAGCGGCCCA CACAGGGCCG 
GGCTATGGGG AGCAGCCACC CAAGAAGCTG GAGGCGGCGT CCGAGGGCAA GGGCGCCGAG 
GCTGTCAGCC AGTGGCTTGC CACGTTCCAG CTGCAGCTGT ACGCCCCCAA CTTCATCAGC 
GCCGGGTATG ACCTGCCCAC CATCAGCCGC ATGACCCCCG AGGACCTCAC TGCCATCGGG 
GTCACCAAGC CCGGCCACCG GAAGAAAATC ACCGCGGAGA TCAGTGGTCT GAGCCTCCCG 
GACTGGCTGC CCGAGCACAA ACCTGCGAAC CTGGCCGTGT GGCTGTCCAT GATCGGCCTG 
GCGCAGTACT ACAAGGTGCT GGTGGACAAC GGCTACGAGA ACATTGACTT CATCACGGAC 
ATCACTTGGG AGGACCTGCA GGAGATCGGC ATCACCAAGC TGGGCCACCA GAAGAAGCTG 
ATGCTGGCTG TGCGGAAACT GGCGGAGCTG CAGAAGGCAG AGTACGCCAA GTACGAGGGG 
GGACCCCTGC GCCGGAAGGC ACCGCAGTCG CTGGAGGTGA TGGCCATCGA GTCGCCACCC 
CCACCCGAGC CTGCCCCGGC TGACTGCCAG TCTCCGAAGA TGACCACCTT CCAGGACAGC 
GAGCTCAGCG GGGAGCTGCA GGCGGCCTTG ACCGGCCCGA CCGAAGGGGC TGCTGCTGCC 
ACCGCCCCGG CTGAAAAGCC CGCCAACCAC TTGCCACCCG CCCCGAGGGC CTCGGGGCGG 
CAGGAGCCCA GCCTGGGGGG GAGGGCGCGG CACATGAGCA GCTCCCAGGA GCTGCTGGGC 
GACGGGCCCC TGGGACCCAG CAGCCCCATG TCACGGAGCC AGGAGTACCT GCTCGATGAG 
GGGCCGGCCC CTGGCACCCC TCCCAAGGAA GCCCGGCCCA GCCGCCACGG CCACAGTGTC 
AAGCGGGCCA GTGTGCCCCC GGTGCCCGGC AAGCCTCGGC AGGTCCTGCC GCCAGGGGCC 
AGCCACTTCA CGCCCCCCCA GACACCCACG AAACCCCGGC CAAGCTCCCC GCAGGCCCTG 
GGGGGGCCTC ATGGCCCAGC GCCAGCCACA GCCAAGGTGA AGCCCACCCC TCAGCTGCTG 
CCGCCCACGG AGCGACCCAT GTCCCCCCGC TCGCTGCCTC AGTCGCCCAC TCACCGCGGC 
TTTGCCTATG TGCTTCCCCA ACCGGTGGAG AGCGAGGCTG GGCCGGCTGC CCCAGGGCCT 
GCACCCGCAG CCGCGCCCAC GCCGGTGCCC ACGCTGTGCC TGCCGCCCGA GGCCGACGCA 
GAGCCAGGGA GGCCCAAGAA GCGTGCCCAC AGCCTGAATC GCTACGCAGC GTCGGACAGC 
GAGCCGGAGC GGGACGAGTT GCTGGTGCCG GCCGCCGCAG GGCCCTACGC CACGGTCCAG 
CGGCGCGTGG GCCGCAGCCA CTCAGTGCGG GCACCTGCCG GCGCAGACAA GAACGTCAAC 
CGCAGCCAGT CGTTTGCCGT GCGGCCGCGG AAGAAGGGGC CGCCCCCACC CCCACCCAAG 
CGCTCCAGCT CTGCCATGGC TAGTGCCAAC CTGGCCGATG AGCCGGCGCC AGACGCAGAG 
ACCGAGGGGG CCGGGGCTGA GGACAGCCGG CTGGGGGTCC GGGCACAGCG TCGGCGGGCC 
AGTGACCTGG CTGGCAGCGT GGACACAGGA AGTGCCGGCA GCGTGAAGAG CATCGCAGCC 
ATGCTTGAGC TGTCATCCAT CGGGGGTGGG GGCCGGGCAG CCCGTAGGCC CCCTGAGGGC 
CATCCCATGC CTCACCCTGC CAGCCCGGAG CCAGGCCGGG TGGCCACGGT GCTGGCCTCA 
GTGAAGCACA AGGAGGCCAT TGGACCTGAC GGCGAGGTGG TGAACCGGCG CCGCACACTG 
AGTGGGCCCG TCACGGGACT CCTGGCCACT GCTCGCCGGG GTTCCGGAGA GCCATCAGGG 
CCTGCAGACC ATGGCCAGTT GGTGGAAGAG GGTGCCACCC GGCAGCGGCC CCGAGGTCCA 
GCCAAGGGCG AGGCGGGTGC AGAGGGCCCG CCCCTAGCCA GGGTGGAGGC CAGTGCCACC 
CTCAAGAGGC GCATCCGGGC CAAGCAGAGC CAGCAGGAGA ACGTCAAGTT CATCCTAACC 
GAGTCTGACA CGGTCAAGCG CCGGCCCAAG GTCAAGGAGC GGGAGGCGGG TCCCGAGCCT 
CCCCCACCAC CGCTGTCCGT GTACCAGAAT GGAACGGGCA CTGTGCGCCG CCGGCCGGCC 
TCTGAGCAGG CTGGGCCCCC GGAGCTGCCC CCGCCACCCC CGCCTGCTGA GCCCCCGCCC 
TCTGACCTCA TGCACCTGCC CCCGCTGCCC CCGCCGGACA GCGATGCCCG GAAGCTGGCC 
AAGCCACCTG TTTCTCCCAA GCCCGTTCTG GCTCAGCCCG TGCCCAAGAT CCAGGGCTCA 
CCCACACCTG CCTCTAAGAA GGTGCCGCTG CCAGGTCCCA GCAGCCCAGA GGTGAAGCGT 
GCGCACGGCA CGCCGCCGCC CGTGTCTCCC AAGCCGCCGC CACCGCCCAC GGCGCCAAAG 
CCGGCCAAGG CCGCGGCGGG GCTGCAGTCG GGCAGTGCCA GCCCGTCGCC CGCCCCCTCG 
CCGGCGCGCC AGCAACCCGC CGCCCTCGCC AAGCCGGCCA GCACGCCGCC CGCGCTGAGC 
GCCAGCCCCG CCAGCCCCGC CAGGCCGCCG TCGCCCGGTG CGCCCGCGCT GCACGTGCCC 
GCCAAGCCGC CGCGCGCTGC CGCCGCGGCG ACAGGGCCCC CAGCTGCGCC CGACGGCGCC 
TCGCCGGGGG ACAGCGCCCG GCAGAAGCTG GAGGAGACGA GCGCGTGCCT GGCGGCGGCG 
CTGCAAGCCG TAGAAGAGAA GATCCGGCAG GAGGACGCGC AAGGCTCGCG CCCCTCGGCC 
GCGGAGGAGA AGAGCACCGG CAGCATCCTG GACGACATTG GCAGCATGTT TGACGACCTG 
GCCGACCAGC TGGACGCCAT GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130779.1
CDS	57..4466
Translation	MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELITLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCTALLEPRPGDTTDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDNGINAHVRNTYSQTALDIVHQFTTSQASKEIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGPRAGAEPSPPQGGSSAGPAAPPEEIWVLRKPFAGGDRSGSLSSVAGGRSGGGHTLHAGSEGVKVGGVAAGPGCLGLEQHWPASLSGFQLLATVLSQKPVSDSSPGDSPVKPPEGPAGATRAQPPAAHTGPGYGEQPPKKLEAASEGKGAEAVSQWLATFQLQLYAPNFISAGYDLPTISRMTPEDLTAIGVTKPGHRKKITAEISGLSLPDWLPEHKPANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPAPADCQSPKMTTFQDSELSGELQAALTGPTEGAAAATAPAEKPANHLPPAPRASGRQEPSLGGRARHMSSSQELLGDGPLGPSSPMSRSQEYLLDEGPAPGTPPKEARPSRHGHSVKRASVPPVPGKPRQVLPPGASHFTPPQTPTKPRPSSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVESEAGPAAPGPAPAAAPTPVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSAMASANLADEPAPDAETEGAGAEDSRLGVRAQRRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPMPHPASPEPGRVATVLASVKHKEAIGPDGEVVNRRRTLSGPVTGLLATARRGSGEPSGPADHGQLVEEGATRQRPRGPAKGEAGAEGPPLARVEASATLKRRIRAKQSQQENVKFILTESDTVKRRPKVKEREAGPEPPPPPLSVYQNGTGTVRRRPASEQAGPPELPPPPPPAEPPPSDLMHLPPLPPPDSDARKLAKPPVSPKPVLAQPVPKIQGSPTPASKKVPLPGPSSPEVKRAHGTPPPVSPKPPPPPTAPKPAKAAAGLQSGSASPSPAPSPARQQPAALAKPASTPPALSASPASPARPPSPGAPALHVPAKPPRAAAAATGPPAAPDGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGSRPSAAEEKSTGSILDDIGSMFDDLADQLDAMLE

Target ORF information:

RefSeq Version	XM_025274994.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274994.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGG TAGGTGGGGT GGCGGCAGGG 
CCAGGGTGCC TGGGTCTGGA GCAGCACTGG CCAGCCAGCC TGTCTGGGTT CCAGCTCCTG 
GCAACGGTGC TCTCCCAGAA GCCGGTGTCC GACTCCAGCC CCGGGGACAG CCCGGTCAAG 
CCTCCGGAGG GCCCTGCAGG TGCGACCCGG GCCCAGCCTC CAGCGGCCCA CACAGGGCCG 
GGCTATGGGG AGCAGCCACC CAAGAAGCTG GAGGCGGCGT CCGAGGGCAA GGGCGCCGAG 
GCTGTCAGCC AGTGGCTTGC CACGTTCCAG CTGCAGCTGT ACGCCCCCAA CTTCATCAGC 
GCCGGGTATG ACCTGCCCAC CATCAGCCGC ATGACCCCCG AGGACCTCAC TGCCATCGGG 
GTCACCAAGC CCGGCCACCG GAAGAAAATC ACCGCGGAGA TCAGTGGTCT GAGCCTCCCG 
GACTGGCTGC CCGAGCACAA ACCTGCGAAC CTGGCCGTGT GGCTGTCCAT GATCGGCCTG 
GCGCAGTACT ACAAGGTGCT GGTGGACAAC GGCTACGAGA ACATTGACTT CATCACGGAC 
ATCACTTGGG AGGACCTGCA GGAGATCGGC ATCACCAAGC TGGGCCACCA GAAGAAGCTG 
ATGCTGGCTG TGCGGAAACT GGCGGAGCTG CAGAAGGCAG AGTACGCCAA GTACGAGGGG 
GGACCCCTGC GCCGGAAGGC ACCGCAGTCG CTGGAGGTGA TGGCCATCGA GTCGCCACCC 
CCACCCGAGC CTGCCCCGGC TGACTGCCAG TCTCCGAAGA TGACCACCTT CCAGGACAGC 
GAGCTCAGCG GGGAGCTGCA GGCGGCCTTG ACCGGCCCGA CCGAAGGGGC TGCTGCTGCC 
ACCGCCCCGG CTGAAAAGCC CGCCAACCAC TTGCCACCCG CCCCGAGGGC CTCGGGGCGG 
CAGGAGCCCA GCCTGGGGGG GAGGGCGCGG CACATGAGCA GCTCCCAGGA GCTGCTGGGC 
GACGGGCCCC TGGGACCCAG CAGCCCCATG TCACGGAGCC AGGAGTACCT GCTCGATGAG 
GGGCCGGCCC CTGGCACCCC TCCCAAGGAA GCCCGGCCCA GCCGCCACGG CCACAGTGTC 
AAGCGGGCCA GTGTGCCCCC GGTGCCCGGC AAGCCTCGGC AGGTCCTGCC GCCAGGGGCC 
AGCCACTTCA CGCCCCCCCA GACACCCACG AAACCCCGGC CAAGCTCCCC GCAGGCCCTG 
GGGGGGCCTC ATGGCCCAGC GCCAGCCACA GCCAAGGTGA AGCCCACCCC TCAGCTGCTG 
CCGCCCACGG AGCGACCCAT GTCCCCCCGC TCGCTGCCTC AGTCGCCCAC TCACCGCGGC 
TTTGCCTATG TGCTTCCCCA ACCGGTGGAG AGCGAGGCTG GGCCGGCTGC CCCAGGGCCT 
GCACCCGCAG CCGCGCCCAC GCCGGTGCCC ACGCTGTGCC TGCCGCCCGA GGCCGACGCA 
GAGCCAGGGA GGCCCAAGAA GCGTGCCCAC AGCCTGAATC GCTACGCAGC GTCGGACAGC 
GAGCCGGAGC GGGACGAGTT GCTGGTGCCG GCCGCCGCAG GGCCCTACGC CACGGTCCAG 
CGGCGCGTGG GCCGCAGCCA CTCAGTGCGG GCACCTGCCG GCGCAGACAA GAACGTCAAC 
CGCAGCCAGT CGTTTGCCGT GCGGCCGCGG AAGAAGGGGC CGCCCCCACC CCCACCCAAG 
CGCTCCAGCT CTGCCATGGC TAGTGCCAAC CTGGCCGATG AGCCGGCGCC AGACGCAGAG 
ACCGAGGGGG CCGGGGCTGA GGACAGCCGG CTGGGGGTCC GGGCACAGCG TCGGCGGGCC 
AGTGACCTGG CTGGCAGCGT GGACACAGGA AGTGCCGGCA GCGTGAAGAG CATCGCAGCC 
ATGCTTGAGC TGTCATCCAT CGGGGGTGGG GGCCGGGCAG CCCGTAGGCC CCCTGAGGGC 
CATCCCATGC CTCACCCTGC CAGCCCGGAG CCAGGCCGGG TGGCCACGGT GCTGGCCTCA 
GTGAAGCACA AGGAGGCCAT TGGACCTGAC GGCGAGGTGG TGAACCGGCG CCGCACACTG 
AGTGGGCCCG TCACGGGACT CCTGGCCACT GCTCGCCGGG GTTCCGGAGA GCCATCAGGG 
CCTGCAGACC ATGGCCAGTT GGTGGAAGAG GGTGCCACCC GGCAGCGGCC CCGAGGTCCA 
GCCAAGGGCG AGGCGGGTGC AGAGGGCCCG CCCCTAGCCA GGGTGGAGGC CAGTGCCACC 
CTCAAGAGGC GCATCCGGGC CAAGCAGAGC CAGCAGGAGA ACGTCAAGTT CATCCTAACC 
GAGTCTGACA CGGTCAAGCG CCGGCCCAAG GTCAAGGAGC GGGAGGCGGG TCCCGAGCCT 
CCCCCACCAC CGCTGTCCGT GTACCAGAAT GGAACGGGCA CTGTGCGCCG CCGGCCGGCC 
TCTGAGCAGG CTGGGCCCCC GGAGCTGCCC CCGCCACCCC CGCCTGCTGA GCCCCCGCCC 
TCTGACCTCA TGCACCTGCC CCCGCTGCCC CCGCCGGACA GCGATGCCCG GAAGCTGGCC 
AAGCCACCTG TTTCTCCCAA GCCCGTTCTG GCTCAGCCCG TGCCCAAGAT CCAGGGCTCA 
CCCACACCTG CCTCTAAGAA GGTGCCGCTG CCAGGTCCCA GCAGCCCAGA GGTGAAGCGT 
GCGCACGGCA CGCCGCCGCC CGTGTCTCCC AAGCCGCCGC CACCGCCCAC GGCGCCAAAG 
CCGGCCAAGG CCGCGGCGGG GCTGCAGTCG GGCAGTGCCA GCCCGTCGCC CGCCCCCTCG 
CCGGCGCGCC AGCAACCCGC CGCCCTCGCC AAGCCGGCCA GCACGCCGCC CGCGCTGAGC 
GCCAGCCCCG CCAGCCCCGC CAGGCCGCCG TCGCCCGGTG CGCCCGCGCT GCACGTGCCC 
GCCAAGCCGC CGCGCGCTGC CGCCGCGGCG ACAGGGCCCC CAGCTGCGCC CGACGGCGCC 
TCGCCGGGGG ACAGCGCCCG GCAGAAGCTG GAGGAGACGA GCGCGTGCCT GGCGGCGGCG 
CTGCAAGCCG TAGAAGAGAA GATCCGGCAG GAGGACGCGC AAGGCTCGCG CCCCTCGGCC 
GCGGAGGAGA AGAGCACCGG CAGCATCCTG GACGACATTG GCAGCATGTT TGACGACCTG 
GCCGACCAGC TGGACGCCAT GCTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASKIN1 ( XM_025274996.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130781.1 Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu59864
Clone ID Related Accession (Same CDS sequence)	XM_025274996.1
Accession Version	XM_025274996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	caskin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGGCG CCGAGGCTGT CAGCCAGTGG 
CTTGCCACGT TCCAGCTGCA GCTGTACGCC CCCAACTTCA TCAGCGCCGG GTATGACCTG 
CCCACCATCA GCCGCATGAC CCCCGAGGAC CTCACTGCCA TCGGGGTCAC CAAGCCCGGC 
CACCGGAAGA AAATCACCGC GGAGATCAGT GGTCTGAGCC TCCCGGACTG GCTGCCCGAG 
CACAAACCTG CGAACCTGGC CGTGTGGCTG TCCATGATCG GCCTGGCGCA GTACTACAAG 
GTGCTGGTGG ACAACGGCTA CGAGAACATT GACTTCATCA CGGACATCAC TTGGGAGGAC 
CTGCAGGAGA TCGGCATCAC CAAGCTGGGC CACCAGAAGA AGCTGATGCT GGCTGTGCGG 
AAACTGGCGG AGCTGCAGAA GGCAGAGTAC GCCAAGTACG AGGGGGGACC CCTGCGCCGG 
AAGGCACCGC AGTCGCTGGA GGTGATGGCC ATCGAGTCGC CACCCCCACC CGAGCCTGCC 
CCGGCTGACT GCCAGTCTCC GAAGATGACC ACCTTCCAGG ACAGCGAGCT CAGCGGGGAG 
CTGCAGGCGG CCTTGACCGG CCCGACCGAA GGGGCTGCTG CTGCCACCGC CCCGGCTGAA 
AAGCCCGCCA ACCACTTGCC ACCCGCCCCG AGGGCCTCGG GGCGGCAGGA GCCCAGCCTG 
GGGGGGAGGG CGCGGCACAT GAGCAGCTCC CAGGAGCTGC TGGGCGACGG GCCCCTGGGA 
CCCAGCAGCC CCATGTCACG GAGCCAGGAG TACCTGCTCG ATGAGGGGCC GGCCCCTGGC 
ACCCCTCCCA AGGAAGCCCG GCCCAGCCGC CACGGCCACA GTGTCAAGCG GGCCAGTGTG 
CCCCCGGTGC CCGGCAAGCC TCGGCAGGTC CTGCCGCCAG GGGCCAGCCA CTTCACGCCC 
CCCCAGACAC CCACGAAACC CCGGCCAAGC TCCCCGCAGG CCCTGGGGGG GCCTCATGGC 
CCAGCGCCAG CCACAGCCAA GGTGAAGCCC ACCCCTCAGC TGCTGCCGCC CACGGAGCGA 
CCCATGTCCC CCCGCTCGCT GCCTCAGTCG CCCACTCACC GCGGCTTTGC CTATGTGCTT 
CCCCAACCGG TGGAGAGCGA GGCTGGGCCG GCTGCCCCAG GGCCTGCACC CGCAGCCGCG 
CCCACGCCGG TGCCCACGCT GTGCCTGCCG CCCGAGGCCG ACGCAGAGCC AGGGAGGCCC 
AAGAAGCGTG CCCACAGCCT GAATCGCTAC GCAGCGTCGG ACAGCGAGCC GGAGCGGGAC 
GAGTTGCTGG TGCCGGCCGC CGCAGGGCCC TACGCCACGG TCCAGCGGCG CGTGGGCCGC 
AGCCACTCAG TGCGGGCACC TGCCGGCGCA GACAAGAACG TCAACCGCAG CCAGTCGTTT 
GCCGTGCGGC CGCGGAAGAA GGGGCCGCCC CCACCCCCAC CCAAGCGCTC CAGCTCTGCC 
ATGGCTAGTG CCAACCTGGC CGATGAGCCG GCGCCAGACG CAGAGACCGA GGGGGCCGGG 
GCTGAGGACA GCCGGCTGGG GGTCCGGGCA CAGCGTCGGC GGGCCAGTGA CCTGGCTGGC 
AGCGTGGACA CAGGAAGTGC CGGCAGCGTG AAGAGCATCG CAGCCATGCT TGAGCTGTCA 
TCCATCGGGG GTGGGGGCCG GGCAGCCCGT AGGCCCCCTG AGGGCCATCC CATGCCTCAC 
CCTGCCAGCC CGGAGCCAGG CCGGGTGGCC ACGGTGCTGG CCTCAGTGAA GCACAAGGAG 
GCCATTGGAC CTGACGGCGA GGTGGTGAAC CGGCGCCGCA CACTGAGTGG GCCCGTCACG 
GGACTCCTGG CCACTGCTCG CCGGGGTTCC GGAGAGCCAT CAGGGCCTGC AGACCATGGC 
CAGTTGGTGG AAGAGGGTGC CACCCGGCAG CGGCCCCGAG GTCCAGCCAA GGGCGAGGCG 
GGTGCAGAGG GCCCGCCCCT AGCCAGGGTG GAGGCCAGTG CCACCCTCAA GAGGCGCATC 
CGGGCCAAGC AGAGCCAGCA GGAGAACGTC AAGTTCATCC TAACCGAGTC TGACACGGTC 
AAGCGCCGGC CCAAGGTCAA GGAGCGGGAG GCGGGTCCCG AGCCTCCCCC ACCACCGCTG 
TCCGTGTACC AGAATGGAAC GGGCACTGTG CGCCGCCGGC CGGCCTCTGA GCAGGCTGGG 
CCCCCGGAGC TGCCCCCGCC ACCCCCGCCT GCTGAGCCCC CGCCCTCTGA CCTCATGCAC 
CTGCCCCCGC TGCCCCCGCC GGACAGCGAT GCCCGGAAGC TGGCCAAGCC ACCTGTTTCT 
CCCAAGCCCG TTCTGGCTCA GCCCGTGCCC AAGATCCAGG GCTCACCCAC ACCTGCCTCT 
AAGAAGGTGC CGCTGCCAGG TCCCAGCAGC CCAGAGGTGA AGCGTGCGCA CGGCACGCCG 
CCGCCCGTGT CTCCCAAGCC GCCGCCACCG CCCACGGCGC CAAAGCCGGC CAAGGCCGCG 
GCGGGGCTGC AGTCGGGCAG TGCCAGCCCG TCGCCCGCCC CCTCGCCGGC GCGCCAGCAA 
CCCGCCGCCC TCGCCAAGCC GGCCAGCACG CCGCCCGCGC TGAGCGCCAG CCCCGCCAGC 
CCCGCCAGGC CGCCGTCGCC CGGTGCGCCC GCGCTGCACG TGCCCGCCAA GCCGCCGCGC 
GCTGCCGCCG CGGCGACAGG GCCCCCAGCT GCGCCCGACG GCGCCTCGCC GGGGGACAGC 
GCCCGGCAGA AGCTGGAGGA GACGAGCGCG TGCCTGGCGG CGGCGCTGCA AGCCGTAGAA 
GAGAAGATCC GGCAGGAGGA CGCGCAAGGC TCGCGCCCCT CGGCCGCGGA GGAGAAGAGC 
ACCGGCAGCA TCCTGGACGA CATTGGCAGC ATGTTTGACG ACCTGGCCGA CCAGCTGGAC 
GCCATGCTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130781.1
CDS	57..4391
Translation	MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELITLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCTALLEPRPGDTTDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDNGINAHVRNTYSQTALDIVHQFTTSQASKEIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGPRAGAEPSPPQGGSSAGPAAPPEEIWVLRKPFAGGDRSGSLSSVAGGRSGGGHTLHAGSEGVKLLATVLSQKPVSDSSPGDSPVKPPEGPAGATRAQPPAAHTGPGYGEQPPKKLEAASEGKGAEAVSQWLATFQLQLYAPNFISAGYDLPTISRMTPEDLTAIGVTKPGHRKKITAEISGLSLPDWLPEHKPANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPAPADCQSPKMTTFQDSELSGELQAALTGPTEGAAAATAPAEKPANHLPPAPRASGRQEPSLGGRARHMSSSQELLGDGPLGPSSPMSRSQEYLLDEGPAPGTPPKEARPSRHGHSVKRASVPPVPGKPRQVLPPGASHFTPPQTPTKPRPSSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVESEAGPAAPGPAPAAAPTPVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSAMASANLADEPAPDAETEGAGAEDSRLGVRAQRRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPMPHPASPEPGRVATVLASVKHKEAIGPDGEVVNRRRTLSGPVTGLLATARRGSGEPSGPADHGQLVEEGATRQRPRGPAKGEAGAEGPPLARVEASATLKRRIRAKQSQQENVKFILTESDTVKRRPKVKEREAGPEPPPPPLSVYQNGTGTVRRRPASEQAGPPELPPPPPPAEPPPSDLMHLPPLPPPDSDARKLAKPPVSPKPVLAQPVPKIQGSPTPASKKVPLPGPSSPEVKRAHGTPPPVSPKPPPPPTAPKPAKAAAGLQSGSASPSPAPSPARQQPAALAKPASTPPALSASPASPARPPSPGAPALHVPAKPPRAAAAATGPPAAPDGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGSRPSAAEEKSTGSILDDIGSMFDDLADQLDAMLE

Target ORF information:

RefSeq Version	XM_025274996.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274996.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGGCG CCGAGGCTGT CAGCCAGTGG 
CTTGCCACGT TCCAGCTGCA GCTGTACGCC CCCAACTTCA TCAGCGCCGG GTATGACCTG 
CCCACCATCA GCCGCATGAC CCCCGAGGAC CTCACTGCCA TCGGGGTCAC CAAGCCCGGC 
CACCGGAAGA AAATCACCGC GGAGATCAGT GGTCTGAGCC TCCCGGACTG GCTGCCCGAG 
CACAAACCTG CGAACCTGGC CGTGTGGCTG TCCATGATCG GCCTGGCGCA GTACTACAAG 
GTGCTGGTGG ACAACGGCTA CGAGAACATT GACTTCATCA CGGACATCAC TTGGGAGGAC 
CTGCAGGAGA TCGGCATCAC CAAGCTGGGC CACCAGAAGA AGCTGATGCT GGCTGTGCGG 
AAACTGGCGG AGCTGCAGAA GGCAGAGTAC GCCAAGTACG AGGGGGGACC CCTGCGCCGG 
AAGGCACCGC AGTCGCTGGA GGTGATGGCC ATCGAGTCGC CACCCCCACC CGAGCCTGCC 
CCGGCTGACT GCCAGTCTCC GAAGATGACC ACCTTCCAGG ACAGCGAGCT CAGCGGGGAG 
CTGCAGGCGG CCTTGACCGG CCCGACCGAA GGGGCTGCTG CTGCCACCGC CCCGGCTGAA 
AAGCCCGCCA ACCACTTGCC ACCCGCCCCG AGGGCCTCGG GGCGGCAGGA GCCCAGCCTG 
GGGGGGAGGG CGCGGCACAT GAGCAGCTCC CAGGAGCTGC TGGGCGACGG GCCCCTGGGA 
CCCAGCAGCC CCATGTCACG GAGCCAGGAG TACCTGCTCG ATGAGGGGCC GGCCCCTGGC 
ACCCCTCCCA AGGAAGCCCG GCCCAGCCGC CACGGCCACA GTGTCAAGCG GGCCAGTGTG 
CCCCCGGTGC CCGGCAAGCC TCGGCAGGTC CTGCCGCCAG GGGCCAGCCA CTTCACGCCC 
CCCCAGACAC CCACGAAACC CCGGCCAAGC TCCCCGCAGG CCCTGGGGGG GCCTCATGGC 
CCAGCGCCAG CCACAGCCAA GGTGAAGCCC ACCCCTCAGC TGCTGCCGCC CACGGAGCGA 
CCCATGTCCC CCCGCTCGCT GCCTCAGTCG CCCACTCACC GCGGCTTTGC CTATGTGCTT 
CCCCAACCGG TGGAGAGCGA GGCTGGGCCG GCTGCCCCAG GGCCTGCACC CGCAGCCGCG 
CCCACGCCGG TGCCCACGCT GTGCCTGCCG CCCGAGGCCG ACGCAGAGCC AGGGAGGCCC 
AAGAAGCGTG CCCACAGCCT GAATCGCTAC GCAGCGTCGG ACAGCGAGCC GGAGCGGGAC 
GAGTTGCTGG TGCCGGCCGC CGCAGGGCCC TACGCCACGG TCCAGCGGCG CGTGGGCCGC 
AGCCACTCAG TGCGGGCACC TGCCGGCGCA GACAAGAACG TCAACCGCAG CCAGTCGTTT 
GCCGTGCGGC CGCGGAAGAA GGGGCCGCCC CCACCCCCAC CCAAGCGCTC CAGCTCTGCC 
ATGGCTAGTG CCAACCTGGC CGATGAGCCG GCGCCAGACG CAGAGACCGA GGGGGCCGGG 
GCTGAGGACA GCCGGCTGGG GGTCCGGGCA CAGCGTCGGC GGGCCAGTGA CCTGGCTGGC 
AGCGTGGACA CAGGAAGTGC CGGCAGCGTG AAGAGCATCG CAGCCATGCT TGAGCTGTCA 
TCCATCGGGG GTGGGGGCCG GGCAGCCCGT AGGCCCCCTG AGGGCCATCC CATGCCTCAC 
CCTGCCAGCC CGGAGCCAGG CCGGGTGGCC ACGGTGCTGG CCTCAGTGAA GCACAAGGAG 
GCCATTGGAC CTGACGGCGA GGTGGTGAAC CGGCGCCGCA CACTGAGTGG GCCCGTCACG 
GGACTCCTGG CCACTGCTCG CCGGGGTTCC GGAGAGCCAT CAGGGCCTGC AGACCATGGC 
CAGTTGGTGG AAGAGGGTGC CACCCGGCAG CGGCCCCGAG GTCCAGCCAA GGGCGAGGCG 
GGTGCAGAGG GCCCGCCCCT AGCCAGGGTG GAGGCCAGTG CCACCCTCAA GAGGCGCATC 
CGGGCCAAGC AGAGCCAGCA GGAGAACGTC AAGTTCATCC TAACCGAGTC TGACACGGTC 
AAGCGCCGGC CCAAGGTCAA GGAGCGGGAG GCGGGTCCCG AGCCTCCCCC ACCACCGCTG 
TCCGTGTACC AGAATGGAAC GGGCACTGTG CGCCGCCGGC CGGCCTCTGA GCAGGCTGGG 
CCCCCGGAGC TGCCCCCGCC ACCCCCGCCT GCTGAGCCCC CGCCCTCTGA CCTCATGCAC 
CTGCCCCCGC TGCCCCCGCC GGACAGCGAT GCCCGGAAGC TGGCCAAGCC ACCTGTTTCT 
CCCAAGCCCG TTCTGGCTCA GCCCGTGCCC AAGATCCAGG GCTCACCCAC ACCTGCCTCT 
AAGAAGGTGC CGCTGCCAGG TCCCAGCAGC CCAGAGGTGA AGCGTGCGCA CGGCACGCCG 
CCGCCCGTGT CTCCCAAGCC GCCGCCACCG CCCACGGCGC CAAAGCCGGC CAAGGCCGCG 
GCGGGGCTGC AGTCGGGCAG TGCCAGCCCG TCGCCCGCCC CCTCGCCGGC GCGCCAGCAA 
CCCGCCGCCC TCGCCAAGCC GGCCAGCACG CCGCCCGCGC TGAGCGCCAG CCCCGCCAGC 
CCCGCCAGGC CGCCGTCGCC CGGTGCGCCC GCGCTGCACG TGCCCGCCAA GCCGCCGCGC 
GCTGCCGCCG CGGCGACAGG GCCCCCAGCT GCGCCCGACG GCGCCTCGCC GGGGGACAGC 
GCCCGGCAGA AGCTGGAGGA GACGAGCGCG TGCCTGGCGG CGGCGCTGCA AGCCGTAGAA 
GAGAAGATCC GGCAGGAGGA CGCGCAAGGC TCGCGCCCCT CGGCCGCGGA GGAGAAGAGC 
ACCGGCAGCA TCCTGGACGA CATTGGCAGC ATGTTTGACG ACCTGGCCGA CCAGCTGGAC 
GCCATGCTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASKIN1 ( XM_025274997.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130782.1 Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X3, mRNA.

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CloneID	OBu59865
Clone ID Related Accession (Same CDS sequence)	XM_025274997.1
Accession Version	XM_025274997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	caskin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGG TAGGTGGGGT GGCGGCAGGG 
CCAGGGTGCC TGGGTCTGGA GCAGCACTGG CCAGCCAGCC TGTCTGGGTT CCAGCTCCTG 
GCAACGGTGC TCTCCCAGAA GCCGGTGTCC GACTCCAGCC CCGGGGACAG CCCGGTCAAG 
CCTCCGGAGG GCCCTGCAGG TGCGACCCGG GCCCAGCCTC CAGCGGCCCA CACAGGGCCG 
GGCTATGGGG AGCAGCCACC CAAGAAGCTG GAGGCGGCGT CCGAGGGCAA GGCGAACCTG 
GCCGTGTGGC TGTCCATGAT CGGCCTGGCG CAGTACTACA AGGTGCTGGT GGACAACGGC 
TACGAGAACA TTGACTTCAT CACGGACATC ACTTGGGAGG ACCTGCAGGA GATCGGCATC 
ACCAAGCTGG GCCACCAGAA GAAGCTGATG CTGGCTGTGC GGAAACTGGC GGAGCTGCAG 
AAGGCAGAGT ACGCCAAGTA CGAGGGGGGA CCCCTGCGCC GGAAGGCACC GCAGTCGCTG 
GAGGTGATGG CCATCGAGTC GCCACCCCCA CCCGAGCCTG CCCCGGCTGA CTGCCAGTCT 
CCGAAGATGA CCACCTTCCA GGACAGCGAG CTCAGCGGGG AGCTGCAGGC GGCCTTGACC 
GGCCCGACCG AAGGGGCTGC TGCTGCCACC GCCCCGGCTG AAAAGCCCGC CAACCACTTG 
CCACCCGCCC CGAGGGCCTC GGGGCGGCAG GAGCCCAGCC TGGGGGGGAG GGCGCGGCAC 
ATGAGCAGCT CCCAGGAGCT GCTGGGCGAC GGGCCCCTGG GACCCAGCAG CCCCATGTCA 
CGGAGCCAGG AGTACCTGCT CGATGAGGGG CCGGCCCCTG GCACCCCTCC CAAGGAAGCC 
CGGCCCAGCC GCCACGGCCA CAGTGTCAAG CGGGCCAGTG TGCCCCCGGT GCCCGGCAAG 
CCTCGGCAGG TCCTGCCGCC AGGGGCCAGC CACTTCACGC CCCCCCAGAC ACCCACGAAA 
CCCCGGCCAA GCTCCCCGCA GGCCCTGGGG GGGCCTCATG GCCCAGCGCC AGCCACAGCC 
AAGGTGAAGC CCACCCCTCA GCTGCTGCCG CCCACGGAGC GACCCATGTC CCCCCGCTCG 
CTGCCTCAGT CGCCCACTCA CCGCGGCTTT GCCTATGTGC TTCCCCAACC GGTGGAGAGC 
GAGGCTGGGC CGGCTGCCCC AGGGCCTGCA CCCGCAGCCG CGCCCACGCC GGTGCCCACG 
CTGTGCCTGC CGCCCGAGGC CGACGCAGAG CCAGGGAGGC CCAAGAAGCG TGCCCACAGC 
CTGAATCGCT ACGCAGCGTC GGACAGCGAG CCGGAGCGGG ACGAGTTGCT GGTGCCGGCC 
GCCGCAGGGC CCTACGCCAC GGTCCAGCGG CGCGTGGGCC GCAGCCACTC AGTGCGGGCA 
CCTGCCGGCG CAGACAAGAA CGTCAACCGC AGCCAGTCGT TTGCCGTGCG GCCGCGGAAG 
AAGGGGCCGC CCCCACCCCC ACCCAAGCGC TCCAGCTCTG CCATGGCTAG TGCCAACCTG 
GCCGATGAGC CGGCGCCAGA CGCAGAGACC GAGGGGGCCG GGGCTGAGGA CAGCCGGCTG 
GGGGTCCGGG CACAGCGTCG GCGGGCCAGT GACCTGGCTG GCAGCGTGGA CACAGGAAGT 
GCCGGCAGCG TGAAGAGCAT CGCAGCCATG CTTGAGCTGT CATCCATCGG GGGTGGGGGC 
CGGGCAGCCC GTAGGCCCCC TGAGGGCCAT CCCATGCCTC ACCCTGCCAG CCCGGAGCCA 
GGCCGGGTGG CCACGGTGCT GGCCTCAGTG AAGCACAAGG AGGCCATTGG ACCTGACGGC 
GAGGTGGTGA ACCGGCGCCG CACACTGAGT GGGCCCGTCA CGGGACTCCT GGCCACTGCT 
CGCCGGGGTT CCGGAGAGCC ATCAGGGCCT GCAGACCATG GCCAGTTGGT GGAAGAGGGT 
GCCACCCGGC AGCGGCCCCG AGGTCCAGCC AAGGGCGAGG CGGGTGCAGA GGGCCCGCCC 
CTAGCCAGGG TGGAGGCCAG TGCCACCCTC AAGAGGCGCA TCCGGGCCAA GCAGAGCCAG 
CAGGAGAACG TCAAGTTCAT CCTAACCGAG TCTGACACGG TCAAGCGCCG GCCCAAGGTC 
AAGGAGCGGG AGGCGGGTCC CGAGCCTCCC CCACCACCGC TGTCCGTGTA CCAGAATGGA 
ACGGGCACTG TGCGCCGCCG GCCGGCCTCT GAGCAGGCTG GGCCCCCGGA GCTGCCCCCG 
CCACCCCCGC CTGCTGAGCC CCCGCCCTCT GACCTCATGC ACCTGCCCCC GCTGCCCCCG 
CCGGACAGCG ATGCCCGGAA GCTGGCCAAG CCACCTGTTT CTCCCAAGCC CGTTCTGGCT 
CAGCCCGTGC CCAAGATCCA GGGCTCACCC ACACCTGCCT CTAAGAAGGT GCCGCTGCCA 
GGTCCCAGCA GCCCAGAGGT GAAGCGTGCG CACGGCACGC CGCCGCCCGT GTCTCCCAAG 
CCGCCGCCAC CGCCCACGGC GCCAAAGCCG GCCAAGGCCG CGGCGGGGCT GCAGTCGGGC 
AGTGCCAGCC CGTCGCCCGC CCCCTCGCCG GCGCGCCAGC AACCCGCCGC CCTCGCCAAG 
CCGGCCAGCA CGCCGCCCGC GCTGAGCGCC AGCCCCGCCA GCCCCGCCAG GCCGCCGTCG 
CCCGGTGCGC CCGCGCTGCA CGTGCCCGCC AAGCCGCCGC GCGCTGCCGC CGCGGCGACA 
GGGCCCCCAG CTGCGCCCGA CGGCGCCTCG CCGGGGGACA GCGCCCGGCA GAAGCTGGAG 
GAGACGAGCG CGTGCCTGGC GGCGGCGCTG CAAGCCGTAG AAGAGAAGAT CCGGCAGGAG 
GACGCGCAAG GCTCGCGCCC CTCGGCCGCG GAGGAGAAGA GCACCGGCAG CATCCTGGAC 
GACATTGGCA GCATGTTTGA CGACCTGGCC GACCAGCTGG ACGCCATGCT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130782.1
CDS	57..4253
Translation	MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELITLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCTALLEPRPGDTTDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDNGINAHVRNTYSQTALDIVHQFTTSQASKEIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGPRAGAEPSPPQGGSSAGPAAPPEEIWVLRKPFAGGDRSGSLSSVAGGRSGGGHTLHAGSEGVKVGGVAAGPGCLGLEQHWPASLSGFQLLATVLSQKPVSDSSPGDSPVKPPEGPAGATRAQPPAAHTGPGYGEQPPKKLEAASEGKANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPAPADCQSPKMTTFQDSELSGELQAALTGPTEGAAAATAPAEKPANHLPPAPRASGRQEPSLGGRARHMSSSQELLGDGPLGPSSPMSRSQEYLLDEGPAPGTPPKEARPSRHGHSVKRASVPPVPGKPRQVLPPGASHFTPPQTPTKPRPSSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVESEAGPAAPGPAPAAAPTPVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSAMASANLADEPAPDAETEGAGAEDSRLGVRAQRRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPMPHPASPEPGRVATVLASVKHKEAIGPDGEVVNRRRTLSGPVTGLLATARRGSGEPSGPADHGQLVEEGATRQRPRGPAKGEAGAEGPPLARVEASATLKRRIRAKQSQQENVKFILTESDTVKRRPKVKEREAGPEPPPPPLSVYQNGTGTVRRRPASEQAGPPELPPPPPPAEPPPSDLMHLPPLPPPDSDARKLAKPPVSPKPVLAQPVPKIQGSPTPASKKVPLPGPSSPEVKRAHGTPPPVSPKPPPPPTAPKPAKAAAGLQSGSASPSPAPSPARQQPAALAKPASTPPALSASPASPARPPSPGAPALHVPAKPPRAAAAATGPPAAPDGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGSRPSAAEEKSTGSILDDIGSMFDDLADQLDAMLE

Target ORF information:

RefSeq Version	XM_025274997.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274997.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGG TAGGTGGGGT GGCGGCAGGG 
CCAGGGTGCC TGGGTCTGGA GCAGCACTGG CCAGCCAGCC TGTCTGGGTT CCAGCTCCTG 
GCAACGGTGC TCTCCCAGAA GCCGGTGTCC GACTCCAGCC CCGGGGACAG CCCGGTCAAG 
CCTCCGGAGG GCCCTGCAGG TGCGACCCGG GCCCAGCCTC CAGCGGCCCA CACAGGGCCG 
GGCTATGGGG AGCAGCCACC CAAGAAGCTG GAGGCGGCGT CCGAGGGCAA GGCGAACCTG 
GCCGTGTGGC TGTCCATGAT CGGCCTGGCG CAGTACTACA AGGTGCTGGT GGACAACGGC 
TACGAGAACA TTGACTTCAT CACGGACATC ACTTGGGAGG ACCTGCAGGA GATCGGCATC 
ACCAAGCTGG GCCACCAGAA GAAGCTGATG CTGGCTGTGC GGAAACTGGC GGAGCTGCAG 
AAGGCAGAGT ACGCCAAGTA CGAGGGGGGA CCCCTGCGCC GGAAGGCACC GCAGTCGCTG 
GAGGTGATGG CCATCGAGTC GCCACCCCCA CCCGAGCCTG CCCCGGCTGA CTGCCAGTCT 
CCGAAGATGA CCACCTTCCA GGACAGCGAG CTCAGCGGGG AGCTGCAGGC GGCCTTGACC 
GGCCCGACCG AAGGGGCTGC TGCTGCCACC GCCCCGGCTG AAAAGCCCGC CAACCACTTG 
CCACCCGCCC CGAGGGCCTC GGGGCGGCAG GAGCCCAGCC TGGGGGGGAG GGCGCGGCAC 
ATGAGCAGCT CCCAGGAGCT GCTGGGCGAC GGGCCCCTGG GACCCAGCAG CCCCATGTCA 
CGGAGCCAGG AGTACCTGCT CGATGAGGGG CCGGCCCCTG GCACCCCTCC CAAGGAAGCC 
CGGCCCAGCC GCCACGGCCA CAGTGTCAAG CGGGCCAGTG TGCCCCCGGT GCCCGGCAAG 
CCTCGGCAGG TCCTGCCGCC AGGGGCCAGC CACTTCACGC CCCCCCAGAC ACCCACGAAA 
CCCCGGCCAA GCTCCCCGCA GGCCCTGGGG GGGCCTCATG GCCCAGCGCC AGCCACAGCC 
AAGGTGAAGC CCACCCCTCA GCTGCTGCCG CCCACGGAGC GACCCATGTC CCCCCGCTCG 
CTGCCTCAGT CGCCCACTCA CCGCGGCTTT GCCTATGTGC TTCCCCAACC GGTGGAGAGC 
GAGGCTGGGC CGGCTGCCCC AGGGCCTGCA CCCGCAGCCG CGCCCACGCC GGTGCCCACG 
CTGTGCCTGC CGCCCGAGGC CGACGCAGAG CCAGGGAGGC CCAAGAAGCG TGCCCACAGC 
CTGAATCGCT ACGCAGCGTC GGACAGCGAG CCGGAGCGGG ACGAGTTGCT GGTGCCGGCC 
GCCGCAGGGC CCTACGCCAC GGTCCAGCGG CGCGTGGGCC GCAGCCACTC AGTGCGGGCA 
CCTGCCGGCG CAGACAAGAA CGTCAACCGC AGCCAGTCGT TTGCCGTGCG GCCGCGGAAG 
AAGGGGCCGC CCCCACCCCC ACCCAAGCGC TCCAGCTCTG CCATGGCTAG TGCCAACCTG 
GCCGATGAGC CGGCGCCAGA CGCAGAGACC GAGGGGGCCG GGGCTGAGGA CAGCCGGCTG 
GGGGTCCGGG CACAGCGTCG GCGGGCCAGT GACCTGGCTG GCAGCGTGGA CACAGGAAGT 
GCCGGCAGCG TGAAGAGCAT CGCAGCCATG CTTGAGCTGT CATCCATCGG GGGTGGGGGC 
CGGGCAGCCC GTAGGCCCCC TGAGGGCCAT CCCATGCCTC ACCCTGCCAG CCCGGAGCCA 
GGCCGGGTGG CCACGGTGCT GGCCTCAGTG AAGCACAAGG AGGCCATTGG ACCTGACGGC 
GAGGTGGTGA ACCGGCGCCG CACACTGAGT GGGCCCGTCA CGGGACTCCT GGCCACTGCT 
CGCCGGGGTT CCGGAGAGCC ATCAGGGCCT GCAGACCATG GCCAGTTGGT GGAAGAGGGT 
GCCACCCGGC AGCGGCCCCG AGGTCCAGCC AAGGGCGAGG CGGGTGCAGA GGGCCCGCCC 
CTAGCCAGGG TGGAGGCCAG TGCCACCCTC AAGAGGCGCA TCCGGGCCAA GCAGAGCCAG 
CAGGAGAACG TCAAGTTCAT CCTAACCGAG TCTGACACGG TCAAGCGCCG GCCCAAGGTC 
AAGGAGCGGG AGGCGGGTCC CGAGCCTCCC CCACCACCGC TGTCCGTGTA CCAGAATGGA 
ACGGGCACTG TGCGCCGCCG GCCGGCCTCT GAGCAGGCTG GGCCCCCGGA GCTGCCCCCG 
CCACCCCCGC CTGCTGAGCC CCCGCCCTCT GACCTCATGC ACCTGCCCCC GCTGCCCCCG 
CCGGACAGCG ATGCCCGGAA GCTGGCCAAG CCACCTGTTT CTCCCAAGCC CGTTCTGGCT 
CAGCCCGTGC CCAAGATCCA GGGCTCACCC ACACCTGCCT CTAAGAAGGT GCCGCTGCCA 
GGTCCCAGCA GCCCAGAGGT GAAGCGTGCG CACGGCACGC CGCCGCCCGT GTCTCCCAAG 
CCGCCGCCAC CGCCCACGGC GCCAAAGCCG GCCAAGGCCG CGGCGGGGCT GCAGTCGGGC 
AGTGCCAGCC CGTCGCCCGC CCCCTCGCCG GCGCGCCAGC AACCCGCCGC CCTCGCCAAG 
CCGGCCAGCA CGCCGCCCGC GCTGAGCGCC AGCCCCGCCA GCCCCGCCAG GCCGCCGTCG 
CCCGGTGCGC CCGCGCTGCA CGTGCCCGCC AAGCCGCCGC GCGCTGCCGC CGCGGCGACA 
GGGCCCCCAG CTGCGCCCGA CGGCGCCTCG CCGGGGGACA GCGCCCGGCA GAAGCTGGAG 
GAGACGAGCG CGTGCCTGGC GGCGGCGCTG CAAGCCGTAG AAGAGAAGAT CCGGCAGGAG 
GACGCGCAAG GCTCGCGCCC CTCGGCCGCG GAGGAGAAGA GCACCGGCAG CATCCTGGAC 
GACATTGGCA GCATGTTTGA CGACCTGGCC GACCAGCTGG ACGCCATGCT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASKIN1 ( XM_025274998.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025130783.1 Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X4, mRNA.

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CloneID	OBu59866
Clone ID Related Accession (Same CDS sequence)	XM_025274998.1
Accession Version	XM_025274998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	caskin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037568.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGCGA ACCTGGCCGT GTGGCTGTCC 
ATGATCGGCC TGGCGCAGTA CTACAAGGTG CTGGTGGACA ACGGCTACGA GAACATTGAC 
TTCATCACGG ACATCACTTG GGAGGACCTG CAGGAGATCG GCATCACCAA GCTGGGCCAC 
CAGAAGAAGC TGATGCTGGC TGTGCGGAAA CTGGCGGAGC TGCAGAAGGC AGAGTACGCC 
AAGTACGAGG GGGGACCCCT GCGCCGGAAG GCACCGCAGT CGCTGGAGGT GATGGCCATC 
GAGTCGCCAC CCCCACCCGA GCCTGCCCCG GCTGACTGCC AGTCTCCGAA GATGACCACC 
TTCCAGGACA GCGAGCTCAG CGGGGAGCTG CAGGCGGCCT TGACCGGCCC GACCGAAGGG 
GCTGCTGCTG CCACCGCCCC GGCTGAAAAG CCCGCCAACC ACTTGCCACC CGCCCCGAGG 
GCCTCGGGGC GGCAGGAGCC CAGCCTGGGG GGGAGGGCGC GGCACATGAG CAGCTCCCAG 
GAGCTGCTGG GCGACGGGCC CCTGGGACCC AGCAGCCCCA TGTCACGGAG CCAGGAGTAC 
CTGCTCGATG AGGGGCCGGC CCCTGGCACC CCTCCCAAGG AAGCCCGGCC CAGCCGCCAC 
GGCCACAGTG TCAAGCGGGC CAGTGTGCCC CCGGTGCCCG GCAAGCCTCG GCAGGTCCTG 
CCGCCAGGGG CCAGCCACTT CACGCCCCCC CAGACACCCA CGAAACCCCG GCCAAGCTCC 
CCGCAGGCCC TGGGGGGGCC TCATGGCCCA GCGCCAGCCA CAGCCAAGGT GAAGCCCACC 
CCTCAGCTGC TGCCGCCCAC GGAGCGACCC ATGTCCCCCC GCTCGCTGCC TCAGTCGCCC 
ACTCACCGCG GCTTTGCCTA TGTGCTTCCC CAACCGGTGG AGAGCGAGGC TGGGCCGGCT 
GCCCCAGGGC CTGCACCCGC AGCCGCGCCC ACGCCGGTGC CCACGCTGTG CCTGCCGCCC 
GAGGCCGACG CAGAGCCAGG GAGGCCCAAG AAGCGTGCCC ACAGCCTGAA TCGCTACGCA 
GCGTCGGACA GCGAGCCGGA GCGGGACGAG TTGCTGGTGC CGGCCGCCGC AGGGCCCTAC 
GCCACGGTCC AGCGGCGCGT GGGCCGCAGC CACTCAGTGC GGGCACCTGC CGGCGCAGAC 
AAGAACGTCA ACCGCAGCCA GTCGTTTGCC GTGCGGCCGC GGAAGAAGGG GCCGCCCCCA 
CCCCCACCCA AGCGCTCCAG CTCTGCCATG GCTAGTGCCA ACCTGGCCGA TGAGCCGGCG 
CCAGACGCAG AGACCGAGGG GGCCGGGGCT GAGGACAGCC GGCTGGGGGT CCGGGCACAG 
CGTCGGCGGG CCAGTGACCT GGCTGGCAGC GTGGACACAG GAAGTGCCGG CAGCGTGAAG 
AGCATCGCAG CCATGCTTGA GCTGTCATCC ATCGGGGGTG GGGGCCGGGC AGCCCGTAGG 
CCCCCTGAGG GCCATCCCAT GCCTCACCCT GCCAGCCCGG AGCCAGGCCG GGTGGCCACG 
GTGCTGGCCT CAGTGAAGCA CAAGGAGGCC ATTGGACCTG ACGGCGAGGT GGTGAACCGG 
CGCCGCACAC TGAGTGGGCC CGTCACGGGA CTCCTGGCCA CTGCTCGCCG GGGTTCCGGA 
GAGCCATCAG GGCCTGCAGA CCATGGCCAG TTGGTGGAAG AGGGTGCCAC CCGGCAGCGG 
CCCCGAGGTC CAGCCAAGGG CGAGGCGGGT GCAGAGGGCC CGCCCCTAGC CAGGGTGGAG 
GCCAGTGCCA CCCTCAAGAG GCGCATCCGG GCCAAGCAGA GCCAGCAGGA GAACGTCAAG 
TTCATCCTAA CCGAGTCTGA CACGGTCAAG CGCCGGCCCA AGGTCAAGGA GCGGGAGGCG 
GGTCCCGAGC CTCCCCCACC ACCGCTGTCC GTGTACCAGA ATGGAACGGG CACTGTGCGC 
CGCCGGCCGG CCTCTGAGCA GGCTGGGCCC CCGGAGCTGC CCCCGCCACC CCCGCCTGCT 
GAGCCCCCGC CCTCTGACCT CATGCACCTG CCCCCGCTGC CCCCGCCGGA CAGCGATGCC 
CGGAAGCTGG CCAAGCCACC TGTTTCTCCC AAGCCCGTTC TGGCTCAGCC CGTGCCCAAG 
ATCCAGGGCT CACCCACACC TGCCTCTAAG AAGGTGCCGC TGCCAGGTCC CAGCAGCCCA 
GAGGTGAAGC GTGCGCACGG CACGCCGCCG CCCGTGTCTC CCAAGCCGCC GCCACCGCCC 
ACGGCGCCAA AGCCGGCCAA GGCCGCGGCG GGGCTGCAGT CGGGCAGTGC CAGCCCGTCG 
CCCGCCCCCT CGCCGGCGCG CCAGCAACCC GCCGCCCTCG CCAAGCCGGC CAGCACGCCG 
CCCGCGCTGA GCGCCAGCCC CGCCAGCCCC GCCAGGCCGC CGTCGCCCGG TGCGCCCGCG 
CTGCACGTGC CCGCCAAGCC GCCGCGCGCT GCCGCCGCGG CGACAGGGCC CCCAGCTGCG 
CCCGACGGCG CCTCGCCGGG GGACAGCGCC CGGCAGAAGC TGGAGGAGAC GAGCGCGTGC 
CTGGCGGCGG CGCTGCAAGC CGTAGAAGAG AAGATCCGGC AGGAGGACGC GCAAGGCTCG 
CGCCCCTCGG CCGCGGAGGA GAAGAGCACC GGCAGCATCC TGGACGACAT TGGCAGCATG 
TTTGACGACC TGGCCGACCA GCTGGACGCC ATGCTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025130783.1
CDS	57..4178
Translation	MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELITLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCTALLEPRPGDTTDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDNGINAHVRNTYSQTALDIVHQFTTSQASKEIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGPRAGAEPSPPQGGSSAGPAAPPEEIWVLRKPFAGGDRSGSLSSVAGGRSGGGHTLHAGSEGVKLLATVLSQKPVSDSSPGDSPVKPPEGPAGATRAQPPAAHTGPGYGEQPPKKLEAASEGKANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPAPADCQSPKMTTFQDSELSGELQAALTGPTEGAAAATAPAEKPANHLPPAPRASGRQEPSLGGRARHMSSSQELLGDGPLGPSSPMSRSQEYLLDEGPAPGTPPKEARPSRHGHSVKRASVPPVPGKPRQVLPPGASHFTPPQTPTKPRPSSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVESEAGPAAPGPAPAAAPTPVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSAMASANLADEPAPDAETEGAGAEDSRLGVRAQRRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPMPHPASPEPGRVATVLASVKHKEAIGPDGEVVNRRRTLSGPVTGLLATARRGSGEPSGPADHGQLVEEGATRQRPRGPAKGEAGAEGPPLARVEASATLKRRIRAKQSQQENVKFILTESDTVKRRPKVKEREAGPEPPPPPLSVYQNGTGTVRRRPASEQAGPPELPPPPPPAEPPPSDLMHLPPLPPPDSDARKLAKPPVSPKPVLAQPVPKIQGSPTPASKKVPLPGPSSPEVKRAHGTPPPVSPKPPPPPTAPKPAKAAAGLQSGSASPSPAPSPARQQPAALAKPASTPPALSASPASPARPPSPGAPALHVPAKPPRAAAAATGPPAAPDGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGSRPSAAEEKSTGSILDDIGSMFDDLADQLDAMLE

Target ORF information:

RefSeq Version	XM_025274998.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CASK interacting protein 1 (CASKIN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025274998.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGTGA GTTTGGCCGC 
GTTGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGCGA ACCTGGCCGT GTGGCTGTCC 
ATGATCGGCC TGGCGCAGTA CTACAAGGTG CTGGTGGACA ACGGCTACGA GAACATTGAC 
TTCATCACGG ACATCACTTG GGAGGACCTG CAGGAGATCG GCATCACCAA GCTGGGCCAC 
CAGAAGAAGC TGATGCTGGC TGTGCGGAAA CTGGCGGAGC TGCAGAAGGC AGAGTACGCC 
AAGTACGAGG GGGGACCCCT GCGCCGGAAG GCACCGCAGT CGCTGGAGGT GATGGCCATC 
GAGTCGCCAC CCCCACCCGA GCCTGCCCCG GCTGACTGCC AGTCTCCGAA GATGACCACC 
TTCCAGGACA GCGAGCTCAG CGGGGAGCTG CAGGCGGCCT TGACCGGCCC GACCGAAGGG 
GCTGCTGCTG CCACCGCCCC GGCTGAAAAG CCCGCCAACC ACTTGCCACC CGCCCCGAGG 
GCCTCGGGGC GGCAGGAGCC CAGCCTGGGG GGGAGGGCGC GGCACATGAG CAGCTCCCAG 
GAGCTGCTGG GCGACGGGCC CCTGGGACCC AGCAGCCCCA TGTCACGGAG CCAGGAGTAC 
CTGCTCGATG AGGGGCCGGC CCCTGGCACC CCTCCCAAGG AAGCCCGGCC CAGCCGCCAC 
GGCCACAGTG TCAAGCGGGC CAGTGTGCCC CCGGTGCCCG GCAAGCCTCG GCAGGTCCTG 
CCGCCAGGGG CCAGCCACTT CACGCCCCCC CAGACACCCA CGAAACCCCG GCCAAGCTCC 
CCGCAGGCCC TGGGGGGGCC TCATGGCCCA GCGCCAGCCA CAGCCAAGGT GAAGCCCACC 
CCTCAGCTGC TGCCGCCCAC GGAGCGACCC ATGTCCCCCC GCTCGCTGCC TCAGTCGCCC 
ACTCACCGCG GCTTTGCCTA TGTGCTTCCC CAACCGGTGG AGAGCGAGGC TGGGCCGGCT 
GCCCCAGGGC CTGCACCCGC AGCCGCGCCC ACGCCGGTGC CCACGCTGTG CCTGCCGCCC 
GAGGCCGACG CAGAGCCAGG GAGGCCCAAG AAGCGTGCCC ACAGCCTGAA TCGCTACGCA 
GCGTCGGACA GCGAGCCGGA GCGGGACGAG TTGCTGGTGC CGGCCGCCGC AGGGCCCTAC 
GCCACGGTCC AGCGGCGCGT GGGCCGCAGC CACTCAGTGC GGGCACCTGC CGGCGCAGAC 
AAGAACGTCA ACCGCAGCCA GTCGTTTGCC GTGCGGCCGC GGAAGAAGGG GCCGCCCCCA 
CCCCCACCCA AGCGCTCCAG CTCTGCCATG GCTAGTGCCA ACCTGGCCGA TGAGCCGGCG 
CCAGACGCAG AGACCGAGGG GGCCGGGGCT GAGGACAGCC GGCTGGGGGT CCGGGCACAG 
CGTCGGCGGG CCAGTGACCT GGCTGGCAGC GTGGACACAG GAAGTGCCGG CAGCGTGAAG 
AGCATCGCAG CCATGCTTGA GCTGTCATCC ATCGGGGGTG GGGGCCGGGC AGCCCGTAGG 
CCCCCTGAGG GCCATCCCAT GCCTCACCCT GCCAGCCCGG AGCCAGGCCG GGTGGCCACG 
GTGCTGGCCT CAGTGAAGCA CAAGGAGGCC ATTGGACCTG ACGGCGAGGT GGTGAACCGG 
CGCCGCACAC TGAGTGGGCC CGTCACGGGA CTCCTGGCCA CTGCTCGCCG GGGTTCCGGA 
GAGCCATCAG GGCCTGCAGA CCATGGCCAG TTGGTGGAAG AGGGTGCCAC CCGGCAGCGG 
CCCCGAGGTC CAGCCAAGGG CGAGGCGGGT GCAGAGGGCC CGCCCCTAGC CAGGGTGGAG 
GCCAGTGCCA CCCTCAAGAG GCGCATCCGG GCCAAGCAGA GCCAGCAGGA GAACGTCAAG 
TTCATCCTAA CCGAGTCTGA CACGGTCAAG CGCCGGCCCA AGGTCAAGGA GCGGGAGGCG 
GGTCCCGAGC CTCCCCCACC ACCGCTGTCC GTGTACCAGA ATGGAACGGG CACTGTGCGC 
CGCCGGCCGG CCTCTGAGCA GGCTGGGCCC CCGGAGCTGC CCCCGCCACC CCCGCCTGCT 
GAGCCCCCGC CCTCTGACCT CATGCACCTG CCCCCGCTGC CCCCGCCGGA CAGCGATGCC 
CGGAAGCTGG CCAAGCCACC TGTTTCTCCC AAGCCCGTTC TGGCTCAGCC CGTGCCCAAG 
ATCCAGGGCT CACCCACACC TGCCTCTAAG AAGGTGCCGC TGCCAGGTCC CAGCAGCCCA 
GAGGTGAAGC GTGCGCACGG CACGCCGCCG CCCGTGTCTC CCAAGCCGCC GCCACCGCCC 
ACGGCGCCAA AGCCGGCCAA GGCCGCGGCG GGGCTGCAGT CGGGCAGTGC CAGCCCGTCG 
CCCGCCCCCT CGCCGGCGCG CCAGCAACCC GCCGCCCTCG CCAAGCCGGC CAGCACGCCG 
CCCGCGCTGA GCGCCAGCCC CGCCAGCCCC GCCAGGCCGC CGTCGCCCGG TGCGCCCGCG 
CTGCACGTGC CCGCCAAGCC GCCGCGCGCT GCCGCCGCGG CGACAGGGCC CCCAGCTGCG 
CCCGACGGCG CCTCGCCGGG GGACAGCGCC CGGCAGAAGC TGGAGGAGAC GAGCGCGTGC 
CTGGCGGCGG CGCTGCAAGC CGTAGAAGAG AAGATCCGGC AGGAGGACGC GCAAGGCTCG 
CGCCCCTCGG CCGCGGAGGA GAAGAGCACC GGCAGCATCC TGGACGACAT TGGCAGCATG 
TTTGACGACC TGGCCGACCA GCTGGACGCC ATGCTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASKIN1 ( XM_006076884.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076946.1 Bubalus bubalis CASK interacting protein 1 (CASKIN1), mRNA.

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CloneID	OBu30348
Clone ID Related Accession (Same CDS sequence)	XM_006076884.1
Accession Version	XM_006076884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	caskin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785716.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGCGA GTTTGGCCGC 
GTCGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGGCG CCGAGGCTGT CAGCCAGTGG 
CTTGCCACGT TCCAGCTGCA GCTGTACGCC CCCAACTTCA TCAGCGCCGG GTATGACCTG 
CCCACCATCA GCCGCATGAC CCCCGAGGAC CTCACTGCCA TCGGGGTCAC CAAGCCCGGC 
CACCGGAAGA AAATCACCGC GGAGATCAGT GGTCTGAGCC TCCCGGACTG GCTGCCCGAG 
CACAAACCTG CGAACCTGGC CGTGTGGCTG TCCATGATCG GCCTGGCGCA GTACTACAAG 
GTGCTGGTGG ACAACGGCTA CGAGAACATT GACTTCATCA CGGACATCAC TTGGGAGGAC 
CTGCAGGAGA TCGGCATCAC CAAGCTGGGC CACCAGAAGA AGCTGATGCT GGCTGTGCGG 
AAACTGGCGG AGCTGCAGAA GGCAGAGTAC GCCAAGTACG AGGGGGGACC CCTGCGCCGG 
AAGGCACCGC AGTCGCTGGA GGTGATGGCC ATCGAGTCGC CACCCCCACC CGAGCCTGCC 
CCGGCTGACT GCCAGTCTCC GAAGATGACC ACCTTCCAGG ACAGCGAGCT CAGCGGGGAG 
CTGCAGGCGG CCTTGACCGG CCCGACCGAA GGGGCTGCTG CTGCCACCGC CCCGGCTGAA 
AAGCCCGCCA ACCACTTGCC ACCCGCCCCG AGGGCCTCGG GGCGGCAGGA GCCCAGCCTG 
GGGGGGAGGG CGCGGCACAT GAGCAGCTCC CAGGAGCTGC TGGGCGACGG GCCCCTGGGA 
CCCAGCAGCC CCATGTCACG GAGCCAGGAG TACCTGCTCG ATGAGGGGCC GGCCCCTGGC 
ACCCCTCCCA AGGAAGCCCG GCCCAGCCGC CACGGCCACA GTGTCAAGCG GGCCAGTGTG 
CCCCCGGTGC CCGGCAAGCC TCGGCAGGTC CTGCCGCCAG GGGCCAGCCA CTTCACGCCC 
CCCCAGACAC CCACGAAACC CCGGCCAAGC TCCCCGCAGG CCCTGGGGGG GCCTCATGGC 
CCAGCGCCAG CCACAGCCAA GGTGAAGCCC ACCCCTCAGC TGCTGCCGCC CACGGAGCGA 
CCCATGTCCC CCCGCTCGCT GCCTCAGTCG CCCACTCACC GCGGCTTTGC CTATGTGCTT 
CCCCAACCGG TGGAGAGCGA GGCTGGGCCG GCTGCCCCAG GGCCTGCACC CGCAGCCGCG 
CCCACGCCGG TGCCCACGCT GTGCCTGCCG CCCGAGGCCG ACGCAGAGCC AGGGAGGCCC 
AAGAAGCGTG CCCACAGCCT GAATCGCTAC GCAGCGTCGG ACAGCGAGCC GGAGCGGGAC 
GAGTTGCTGG TGCCGGCCGC CGCAGGGCCC TACGCCACGG TCCAGCGGCG CGTGGGCCGC 
AGCCACTCAG TGCGGGCACC TGCCGGCGCA GACAAGAACG TCAACCGCAG CCAGTCGTTT 
GCCGTGCGGC CGCGGAAGAA GGGGCCGCCC CCACCCCCAC CCAAGCGCTC CAGCTCTGCC 
ATGGCTAGTG CCAACCTGGC CGATGAGCCG GCGCCAGACG CAGAGACCGA GGGGGCCGGG 
GCTGAGGACA GCCGGCTGGG GGTCCGGGCA CAGCGTCGGC GGGCCAGTGA CCTGGCTGGC 
AGCGTGGACA CAGGAAGTGC CGGCAGCGTG AAGAGCATCG CAGCCATGCT TGAGCTGTCA 
TCCATCGGGG GTGGGGGCCG GGCAGCCCGT AGGCCCCCTG AGGGCCATCC CATGCCTCAC 
CCTGCCAGCC CGGAGCCAGG CCGGGTGGCC ACGGTGCTGG CCTCAGTGAA GCACAAGGAG 
GCCATTGGAC CTGACGGCGA GGTGGTGAAC CGGCGCCGCA CACTGAGTGG GCCCGTCACG 
GGACTCCTGG CCACTGCTCG CCGGGGTTCC GGAGAGCCAT CAGGGCCTGC AGACCATGGC 
CAGTTGGTGG AAGAGGGTGC CACCCGGCAG CGGCCCCGAG GTCCAGCCAA GGGCGAGGCG 
GGTGCAGAGG GCCCGCCCCT AGCCAGGGTG GAGGCCAGTG CCACCCTCAA GAGGCGCATC 
CGGGCCAAGC AGAGCCAGCA GGAGAACGTC AAGTTCATCC TAACCGAGTC TGACACGGTC 
AAGCGCCGGC CCAAGGTCAA GGAGCGGGAG GCGGGTCCCG AGCCTCCCCC ACCACCGCTG 
TCCGTGTACC AGAATGGAAC GGGCACTGTG CGCCGCCGGC CGGCCTCTGA GCAGGCTGGG 
CCCCCGGAGC TGCCCCCGCC ACCCCCGCCT GCTGAGCCCC CGCCCTCTGA CCTCATGCAC 
CTGCCCCCGC TGCCCCCGCC GGACAGCGAT GCCCGGAAGC TGGCCAAGCC ACCTGTTTCT 
CCCAAGCCCG TTCTGGCTCA GCCCGTGCCC AAGATCCAGG GCTCACCCAC ACCTGCCTCT 
AAGAAGGTGC CGCTGCCAGG TCCCAGCAGC CCAGAGGTGA AGCGTGCGCA CGGCACGCCG 
CCGCCCGTGT CTCCCAAGCC GCCGNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCAGC TGCGCCCGAC 
GGCGCCTCGC CGGGGGACAG CGCCCGGCAG AAGCTGGAGG AGACGAGCGC GTGCCTGGCG 
GCGGCGCTGC AAGCCGTAGA AGAGAAGATC CGGCAGGAGG ACGCGCAAGG CTCGCGCCCC 
TCGGCCGCGG AGGAGAAGAG CACCGGCAGC ATCCTGGACG ACATTGGCAG CATGTTTGAC 
GACCTGGCCG ACCAGCTGGA CGCCATGCTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076946.1
CDS	14..4189
Translation	MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELITLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCTALLEPRPGDTTDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDNGINAHVRNTYSQTALDIVHQFTTSQASKEIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGPRAGAEPSPPQGGSSAGPAAPPEEIWVLRKPFAGGDRSGSLSSVAGGRSGGGHTLHAGSEGVKLLATVLSQKPVSDSSPGDSPVKPPEGPAGATRAQPPAAHTGPGYGEQPPKKLEAASEGKGAEAVSQWLATFQLQLYAPNFISAGYDLPTISRMTPEDLTAIGVTKPGHRKKITAEISGLSLPDWLPEHKPANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPAPADCQSPKMTTFQDSELSGELQAALTGPTEGAAAATAPAEKPANHLPPAPRASGRQEPSLGGRARHMSSSQELLGDGPLGPSSPMSRSQEYLLDEGPAPGTPPKEARPSRHGHSVKRASVPPVPGKPRQVLPPGASHFTPPQTPTKPRPSSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVESEAGPAAPGPAPAAAPTPVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSAMASANLADEPAPDAETEGAGAEDSRLGVRAQRRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPMPHPASPEPGRVATVLASVKHKEAIGPDGEVVNRRRTLSGPVTGLLATARRGSGEPSGPADHGQLVEEGATRQRPRGPAKGEAGAEGPPLARVEASATLKRRIRAKQSQQENVKFILTESDTVKRRPKVKEREAGPEPPPPPLSVYQNGTGTVRRRPASEQAGPPELPPPPPPAEPPPSDLMHLPPLPPPDSDARKLAKPPVSPKPVLAQPVPKIQGSPTPASKKVPLPGPSSPEVKRAHGTPPPVSPKPPnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullnullAAPDGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGSRPSAAEEKSTGSILDDIGSMFDDLADQLDAMLE

Target ORF information:

RefSeq Version	XM_006076884.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis CASK interacting protein 1 (CASKIN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076884.1

ORF Insert Sequence:

ATGGGGAAGG AGCAGGAGCT GGTGCAGGCA GTGAAGGCGG AGGACGTGGG GACCGCGCAG 
AGGCTGCTGC AGAGGCCGCG GCCCGGGAAG GCCAAACTCC TGGGCTCCAC CAAGAAGATC 
AATGTCAATT TCCAAGACCC TGATGGCTTT TCAGCCCTGC ACCACGCGGC CCTGAATGGC 
AACACAGAGC TGATCACCTT GCTGCTGGAG GCCCAGGCTG CTGTGGACAT CAAGGACAAC 
AAAGGCATGC GGCCTCTGCA CTACGCGGCC TGGCAGGGCC GGAAGGAGCC CATGAAGCTG 
GTGCTGAAGG CGGGCTCAGC GGTGAACATC CCGTCCGACG AGGGCCACAT CCCCCTGCAC 
TTGGCGGCCC AGCACGGTCA CTATGATGTG TCCGAAATGC TGCTGCAGCA CCAGTCCAAC 
CCCTGCATGG TGGACAACTC GGGCAAGACG CCCCTGGACC TGGCCTGCGA GTTTGGCCGC 
GTCGGGGTGG TCCAGCTGCT TCTGAGCAGC AACATGTGTA CGGCCTTGCT GGAGCCCCGG 
CCAGGGGACA CCACCGACCC CAACGGCACC AGCCCCCTGC ACCTGGCAGC CAAGAACGGC 
CACATCGACA TCATCAGACT GCTGCTCCAA GCCGGCATCG ACATTAACCG CCAGACCAAG 
TCCGGCACCG CCCTGCACGA GGCCGCGCTC TGTGGGAAGA CGGAGGTGGT TCGGCTGCTG 
CTGGATAATG GGATCAACGC CCACGTGAGG AACACCTACA GCCAGACCGC CCTGGACATT 
GTGCATCAGT TCACCACCTC CCAGGCCAGC AAGGAGATCA AGCAGCTGCT GCGAGAGGCC 
TCGGCGGCCC TGCAGGTCCG GGCAACCAAG GATTACTGCA ACAACTATGA CCTGACCAGC 
CTCAACGTGA AAGCTGGGGA CATCATCACA GTCCTTGAGC AGCACCCAGA TGGCCGCTGG 
AAGGGCTGTA TCCATGACAA CAGGACAGGC AATGACCGTG TGGGCTACTT CCCATCTTCC 
CTGGGCGAGG CCATCGTCAA GCGAGCAGGT CCCCGAGCAG GTGCTGAACC AAGTCCACCC 
CAGGGAGGCA GCTCCGCGGG GCCCGCTGCA CCCCCCGAAG AGATCTGGGT GCTGAGGAAG 
CCTTTTGCAG GTGGGGACCG CAGCGGCAGC TTGAGCAGCG TGGCTGGGGG CCGGAGCGGC 
GGGGGCCACA CCCTGCACGC TGGCTCTGAA GGGGTCAAGC TCCTGGCAAC GGTGCTCTCC 
CAGAAGCCGG TGTCCGACTC CAGCCCCGGG GACAGCCCGG TCAAGCCTCC GGAGGGCCCT 
GCAGGTGCGA CCCGGGCCCA GCCTCCAGCG GCCCACACAG GGCCGGGCTA TGGGGAGCAG 
CCACCCAAGA AGCTGGAGGC GGCGTCCGAG GGCAAGGGCG CCGAGGCTGT CAGCCAGTGG 
CTTGCCACGT TCCAGCTGCA GCTGTACGCC CCCAACTTCA TCAGCGCCGG GTATGACCTG 
CCCACCATCA GCCGCATGAC CCCCGAGGAC CTCACTGCCA TCGGGGTCAC CAAGCCCGGC 
CACCGGAAGA AAATCACCGC GGAGATCAGT GGTCTGAGCC TCCCGGACTG GCTGCCCGAG 
CACAAACCTG CGAACCTGGC CGTGTGGCTG TCCATGATCG GCCTGGCGCA GTACTACAAG 
GTGCTGGTGG ACAACGGCTA CGAGAACATT GACTTCATCA CGGACATCAC TTGGGAGGAC 
CTGCAGGAGA TCGGCATCAC CAAGCTGGGC CACCAGAAGA AGCTGATGCT GGCTGTGCGG 
AAACTGGCGG AGCTGCAGAA GGCAGAGTAC GCCAAGTACG AGGGGGGACC CCTGCGCCGG 
AAGGCACCGC AGTCGCTGGA GGTGATGGCC ATCGAGTCGC CACCCCCACC CGAGCCTGCC 
CCGGCTGACT GCCAGTCTCC GAAGATGACC ACCTTCCAGG ACAGCGAGCT CAGCGGGGAG 
CTGCAGGCGG CCTTGACCGG CCCGACCGAA GGGGCTGCTG CTGCCACCGC CCCGGCTGAA 
AAGCCCGCCA ACCACTTGCC ACCCGCCCCG AGGGCCTCGG GGCGGCAGGA GCCCAGCCTG 
GGGGGGAGGG CGCGGCACAT GAGCAGCTCC CAGGAGCTGC TGGGCGACGG GCCCCTGGGA 
CCCAGCAGCC CCATGTCACG GAGCCAGGAG TACCTGCTCG ATGAGGGGCC GGCCCCTGGC 
ACCCCTCCCA AGGAAGCCCG GCCCAGCCGC CACGGCCACA GTGTCAAGCG GGCCAGTGTG 
CCCCCGGTGC CCGGCAAGCC TCGGCAGGTC CTGCCGCCAG GGGCCAGCCA CTTCACGCCC 
CCCCAGACAC CCACGAAACC CCGGCCAAGC TCCCCGCAGG CCCTGGGGGG GCCTCATGGC 
CCAGCGCCAG CCACAGCCAA GGTGAAGCCC ACCCCTCAGC TGCTGCCGCC CACGGAGCGA 
CCCATGTCCC CCCGCTCGCT GCCTCAGTCG CCCACTCACC GCGGCTTTGC CTATGTGCTT 
CCCCAACCGG TGGAGAGCGA GGCTGGGCCG GCTGCCCCAG GGCCTGCACC CGCAGCCGCG 
CCCACGCCGG TGCCCACGCT GTGCCTGCCG CCCGAGGCCG ACGCAGAGCC AGGGAGGCCC 
AAGAAGCGTG CCCACAGCCT GAATCGCTAC GCAGCGTCGG ACAGCGAGCC GGAGCGGGAC 
GAGTTGCTGG TGCCGGCCGC CGCAGGGCCC TACGCCACGG TCCAGCGGCG CGTGGGCCGC 
AGCCACTCAG TGCGGGCACC TGCCGGCGCA GACAAGAACG TCAACCGCAG CCAGTCGTTT 
GCCGTGCGGC CGCGGAAGAA GGGGCCGCCC CCACCCCCAC CCAAGCGCTC CAGCTCTGCC 
ATGGCTAGTG CCAACCTGGC CGATGAGCCG GCGCCAGACG CAGAGACCGA GGGGGCCGGG 
GCTGAGGACA GCCGGCTGGG GGTCCGGGCA CAGCGTCGGC GGGCCAGTGA CCTGGCTGGC 
AGCGTGGACA CAGGAAGTGC CGGCAGCGTG AAGAGCATCG CAGCCATGCT TGAGCTGTCA 
TCCATCGGGG GTGGGGGCCG GGCAGCCCGT AGGCCCCCTG AGGGCCATCC CATGCCTCAC 
CCTGCCAGCC CGGAGCCAGG CCGGGTGGCC ACGGTGCTGG CCTCAGTGAA GCACAAGGAG 
GCCATTGGAC CTGACGGCGA GGTGGTGAAC CGGCGCCGCA CACTGAGTGG GCCCGTCACG 
GGACTCCTGG CCACTGCTCG CCGGGGTTCC GGAGAGCCAT CAGGGCCTGC AGACCATGGC 
CAGTTGGTGG AAGAGGGTGC CACCCGGCAG CGGCCCCGAG GTCCAGCCAA GGGCGAGGCG 
GGTGCAGAGG GCCCGCCCCT AGCCAGGGTG GAGGCCAGTG CCACCCTCAA GAGGCGCATC 
CGGGCCAAGC AGAGCCAGCA GGAGAACGTC AAGTTCATCC TAACCGAGTC TGACACGGTC 
AAGCGCCGGC CCAAGGTCAA GGAGCGGGAG GCGGGTCCCG AGCCTCCCCC ACCACCGCTG 
TCCGTGTACC AGAATGGAAC GGGCACTGTG CGCCGCCGGC CGGCCTCTGA GCAGGCTGGG 
CCCCCGGAGC TGCCCCCGCC ACCCCCGCCT GCTGAGCCCC CGCCCTCTGA CCTCATGCAC 
CTGCCCCCGC TGCCCCCGCC GGACAGCGAT GCCCGGAAGC TGGCCAAGCC ACCTGTTTCT 
CCCAAGCCCG TTCTGGCTCA GCCCGTGCCC AAGATCCAGG GCTCACCCAC ACCTGCCTCT 
AAGAAGGTGC CGCTGCCAGG TCCCAGCAGC CCAGAGGTGA AGCGTGCGCA CGGCACGCCG 
CCGCCCGTGT CTCCCAAGCC GCCGNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNCAGC TGCGCCCGAC 
GGCGCCTCGC CGGGGGACAG CGCCCGGCAG AAGCTGGAGG AGACGAGCGC GTGCCTGGCG 
GCGGCGCTGC AAGCCGTAGA AGAGAAGATC CGGCAGGAGG ACGCGCAAGG CTCGCGCCCC 
TCGGCCGCGG AGGAGAAGAG CACCGGCAGC ATCCTGGACG ACATTGGCAG CATGTTTGAC 
GACCTGGCCG ACCAGCTGGA CGCCATGCTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CASKIN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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