ACTN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTN1
Entrez Gene ID	102402259
Full Name	actinin alpha 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006056163.1	XP_006056225.1	alpha-actinin-1 isoform X1
XM_025295300.1	XP_025151085.1	alpha-actinin-1 isoform X2
XM_025295301.1	XP_025151086.1	alpha-actinin-1 isoform X3
XM_025295303.1	XP_025151088.1	alpha-actinin-1 isoform X5
XM_006056164.1	XP_006056226.1	alpha-actinin-1 isoform X2
XM_025295299.1	XP_025151084.1	alpha-actinin-1 isoform X1
XM_025295302.1	XP_025151087.1	alpha-actinin-1 isoform X4

Pathways from BioSystems
Gene Ontology

The following ACTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu13328	XM_006056163.1 Latest version!	Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu13328	XM_025295299.1 Latest version!	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu13329	XM_006056164.1 Latest version!	Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu13329	XM_025295302.1 Latest version!	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu44539	XM_025295300.1 Latest version!	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu44540	XM_025295301.1 Latest version!	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu44541	XM_025295303.1 Latest version!	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTN1 ( XM_006056163.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056225.1 Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu13328
Clone ID Related Accession (Same CDS sequence)	XM_006056163.1 , XM_025295299.1
Accession Version	XM_006056163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784733.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC 
GTGGACCCCA ACCGCCTTGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGC 
GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG 
GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCTCCTGA CCAGGCTGAG 
TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056225.1
CDS	304..3048
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006056163.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056163.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC 
GTGGACCCCA ACCGCCTTGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGC 
GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG 
GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCTCCTGA CCAGGCTGAG 
TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_025295299.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151084.1 Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu13328
Clone ID Related Accession (Same CDS sequence)	XM_006056163.1 , XM_025295299.1
Accession Version	XM_025295299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC 
GTGGACCCCA ACCGCCTTGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGC 
GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG 
GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCTCCTGA CCAGGCTGAG 
TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151084.1
CDS	293..3037
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_025295299.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295299.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GAAGAAGACA GGCATGATGG ACACGGATGA TTTCCGCGCC 
TGCCTTATCT CCATGGGTTA CAACATGGGA GAGGCAGAGT TTGCCCGCAT CATGAGCATC 
GTGGACCCCA ACCGCCTTGG GGTGGTGACG TTCCAGGCCT TCATCGACTT TATGTCTCGC 
GAGACAGCTG ATACAGATAC AGCAGACCAA GTCATGGCTT CCTTCAAGAT CCTGGCCGGG 
GATAAGAACT ACATCACGGT GGACGAGCTG CGCAGAGAGC TGCCTCCTGA CCAGGCTGAG 
TACTGCATCG CGCGAATGGC CCCCTACACC GGCCCCGACG CCGTGCCCGG TGCTCTGGAC 
TACATGTCCT TCTCCACGGC GCTGTACGGC GAGAGTGACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_006056164.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056226.1 Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu13329
Clone ID Related Accession (Same CDS sequence)	XM_006056164.1 , XM_025295302.1
Accession Version	XM_006056164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784733.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC 
ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTTGGGGTG 
GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGCGAGA CAGCTGATAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC 
GAGCTGCGCA GAGAGCTGCC TCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC 
TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056226.1
CDS	304..2964
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006056164.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin, alpha 1 (ACTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056164.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC 
ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTTGGGGTG 
GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGCGAGA CAGCTGATAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC 
GAGCTGCGCA GAGAGCTGCC TCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC 
TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_025295302.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151087.1 Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu13329
Clone ID Related Accession (Same CDS sequence)	XM_006056164.1 , XM_025295302.1
Accession Version	XM_025295302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC 
ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTTGGGGTG 
GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGCGAGA CAGCTGATAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC 
GAGCTGCGCA GAGAGCTGCC TCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC 
TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151087.1
CDS	293..2953
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_025295302.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295302.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGACAGGCA TGATGGACAC GGATGATTTC CGCGCCTGCC TTATCTCCAT GGGTTACAAC 
ATGGGAGAGG CAGAGTTTGC CCGCATCATG AGCATCGTGG ACCCCAACCG CCTTGGGGTG 
GTGACGTTCC AGGCCTTCAT CGACTTTATG TCTCGCGAGA CAGCTGATAC AGATACAGCA 
GACCAAGTCA TGGCTTCCTT CAAGATCCTG GCCGGGGATA AGAACTACAT CACGGTGGAC 
GAGCTGCGCA GAGAGCTGCC TCCTGACCAG GCTGAGTACT GCATCGCGCG AATGGCCCCC 
TACACCGGCC CCGACGCCGT GCCCGGTGCT CTGGACTACA TGTCCTTCTC CACGGCGCTG 
TACGGCGAGA GTGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_025295300.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151085.1 Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu44539
Clone ID Related Accession (Same CDS sequence)	XM_025295300.1
Accession Version	XM_025295300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAGGCA GAGTTTGCCC GCATCATGAG CATCGTGGAC 
CCCAACCGCC TTGGGGTGGT GACGTTCCAG GCCTTCATCG ACTTTATGTC TCGCGAGACA 
GCTGATACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC CGGGGATAAG 
AACTACATCA CGGTGGACGA GCTGCGCAGA GAGCTGCCTC CTGACCAGGC TGAGTACTGC 
ATCGCGCGAA TGGCCCCCTA CACCGGCCCC GACGCCGTGC CCGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151085.1
CDS	292..2970
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_025295300.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295300.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
GATCACTCCG GCACGCTGGG TCCCGAGGAG TTCAAAGCCT GCCTCATCAG CTTGGGTTAT 
GATATTGGCA ACGACCCCCA GGGAGAGGCA GAGTTTGCCC GCATCATGAG CATCGTGGAC 
CCCAACCGCC TTGGGGTGGT GACGTTCCAG GCCTTCATCG ACTTTATGTC TCGCGAGACA 
GCTGATACAG ATACAGCAGA CCAAGTCATG GCTTCCTTCA AGATCCTGGC CGGGGATAAG 
AACTACATCA CGGTGGACGA GCTGCGCAGA GAGCTGCCTC CTGACCAGGC TGAGTACTGC 
ATCGCGCGAA TGGCCCCCTA CACCGGCCCC GACGCCGTGC CCGGTGCTCT GGACTACATG 
TCCTTCTCCA CGGCGCTGTA CGGCGAGAGT GACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_025295301.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151086.1 Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu44540
Clone ID Related Accession (Same CDS sequence)	XM_025295301.1
Accession Version	XM_025295301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC 
AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTTGGG 
GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGCG AGACAGCTGA TACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG 
GACGAGCTGC GCAGAGAGCT GCCTCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC 
CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151086.1
CDS	292..2955
Translation	MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_025295301.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295301.1

ORF Insert Sequence:

ATGGACCATT ATGATTCCCA GCAAACCAAT GACTACATGC AGCCCGAAGA GGACTGGGAT 
CGAGACCTGC TCCTGGACCC AGCGTGGGAG AAGCAGCAGA GAAAGACGTT TACAGCATGG 
TGCAACTCCC ACCTCCGGAA GGCAGGAACA CAGATCGAGA ACATCGAGGA GGACTTCCGG 
GACGGCCTGA AGCTGATGCT GCTGCTGGAG GTCATCTCAG GTGAACGTTT GGCCAAGCCC 
GAGAGAGGCA AAATGAGGGT GCACAAGATC TCCAATGTCA ACAAGGCCTT GGACTTCATA 
GCCAGCAAGG GGGTGAAGCT GGTGTCCATA GGAGCTGAAG AAATTGTGGA TGGGAATGTG 
AAAATGACCC TGGGCATGAT CTGGACCATC ATCCTCCGCT TTGCCATCCA GGACATCTCC 
GTGGAAGAGA CTTCAGCGAA GGAAGGGCTT CTCTTGTGGT GTCAGAGAAA GACAGCCCCT 
TACAAGAATG TCAACATCCA GAACTTCCAC ATAAGCTGGA AGGACGGCCT CGGCTTCTGT 
GCCTTGATCC ACCGACACCG GCCCGAGCTG ATCGACTACG GGAAGCTGCG GAAGGATGAC 
CCACTGACAA ACTTGAACAC AGCTTTCGAT GTAGCGGAGA AGTACCTGGA CATTCCCAAG 
ATGTTGGATG CGGAAGACAT CGTCGGAACC GCCCGCCCAG ACGAGAAAGC CATCATGACT 
TACGTGTCCA GCTTCTACCA CGCCTTCTCT GGAGCCCAGA AGGCCGAGAC AGCAGCCAAT 
CGCATCTGCA AGGTGCTGGC CGTCAACCAG GAGAACGAGC AGCTCATGGA GGACTACGAG 
AAGCTGGCCA GCGACCTGCT GGAATGGATC CGCCGCACCA TCCCATGGCT GGAGAACCGG 
GCACCCGAGA ACACCATGCA CGCCATGCAG CAGAAGCTGG AGGACTTCCG GGACTACCGG 
CGCCTGCACA AGCCGCCCAA GGTGCAGGAG AAGTGCCAGC TGGAGATCAA CTTCAACACG 
CTGCAGACCA AGCTGCGCCT CAGCAACCGG CCCGCCTTCA TGCCCTCAGA GGGCAGGATG 
GTCTCGGACA TCAACAACGC CTGGGGCTGC CTGGAGCAGG CGGAGAAGGG CTATGAGGAG 
TGGCTGCTGA ATGAGATCCG GAGGCTGGAG CGGCTCGACC ACCTGGCAGA GAAGTTCCGA 
CAGAAGGCCT CCATCCACGA GGCCTGGACG GACGGCAAAG AGGCCATGCT GCGGCAGAAG 
GATTACGAGA CGGCTACCCT CTCAGAGATC AAGGCCCTGC TCAAGAAGCA CGAGGCCTTT 
GAGAGTGACC TGGCTGCCCA CCAGGACCGC GTGGAGCAGA TTGCCGCCAT TGCCCAGGAG 
CTCAATGAGC TGGACTATTA CGACTCACCC AGTGTCAACG CCCGGTGCCA AAAGATCTGT 
GACCAGTGGG ACAATCTAGG GGCCCTAACC CAGAAGCGAA GGGAAGCTCT GGAGCGCACG 
GAGAAACTGC TGGAGACCAT TGACCAACTG TACTTGGAGT ACGCTAAGCG GGCCGCACCC 
TTCAACAACT GGATGGAGGG GGCCATGGAG GACCTGCAGG ACACCTTCAT TGTGCACACC 
ATCGAGGAGA TCCAGGGACT GACCACAGCC CATGAGCAGT TCAAAGCCAC CCTCCCCGAT 
GCCGACAAGG AGCGCCTGGC TATCCTGGGC ATCCACAACG AGGTGTCCAA GATTGTCCAG 
ACCTACCACG TCAACATGGC AGGCACCAAC CCCTACACAA CCATCACGCC TCAGGAGATC 
AATGGCAAAT GGGACCATGT GCGGCAGCTG GTGCCACGGA GGGACCAGGC CCTAACGGAG 
GAGCACGCCC GCCAGCAGCA CAACGAAAGG CTACGCAAGC AGTTTGGGGC CCAGGCCAAT 
GTCATTGGGC CCTGGATCCA GACCAAGATG GAGGAGATTG GAAGGATCTC CATTGAGATG 
CACGGGACCC TGGAGGACCA GCTCAACCAC CTGCGGCAAT ACGAGAAGAG CATCGTCAAC 
TACAAGCCCA AGATCGACCA GCTGGAGGGC GACCACCAGC TGATCCAGGA GGCGCTCATC 
TTTGACAACA AGCACACCAA CTACACCATG GAGCACATCC GTGTGGGCTG GGAGCAGCTC 
CTAACCACCA TCGCCAGGAC CATCAATGAG GTGGAGAACC AGATCCTGAC CAGGGATGCC 
AAGGGCATCA GCCAGGAGCA GATGAATGAG TTCCGGGCCT CCTTCAACCA CTTTGACCGG 
AAGAAGACAG GCATGATGGA CACGGATGAT TTCCGCGCCT GCCTTATCTC CATGGGTTAC 
AACATGGGAG AGGCAGAGTT TGCCCGCATC ATGAGCATCG TGGACCCCAA CCGCCTTGGG 
GTGGTGACGT TCCAGGCCTT CATCGACTTT ATGTCTCGCG AGACAGCTGA TACAGATACA 
GCAGACCAAG TCATGGCTTC CTTCAAGATC CTGGCCGGGG ATAAGAACTA CATCACGGTG 
GACGAGCTGC GCAGAGAGCT GCCTCCTGAC CAGGCTGAGT ACTGCATCGC GCGAATGGCC 
CCCTACACCG GCCCCGACGC CGTGCCCGGT GCTCTGGACT ACATGTCCTT CTCCACGGCG 
CTGTACGGCG AGAGTGACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN1 ( XM_025295303.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025151088.1 Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X5, mRNA.

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CloneID	OBu44541
Clone ID Related Accession (Same CDS sequence)	XM_025295303.1
Accession Version	XM_025295303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	alpha-actinin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCTTGGACT TCATAGCCAG CAAGGGGGTG 
AAGCTGGTGT CCATAGGAGC TGAAGAAATT GTGGATGGGA ATGTGAAAAT GACCCTGGGC 
ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCCGTGGA AGAGACTTCA 
GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC 
ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA 
CACCGGCCCG AGCTGATCGA CTACGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG 
AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA 
GACATCGTCG GAACCGCCCG CCCAGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC 
TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG 
CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC 
CTGCTGGAAT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC 
ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT GCACAAGCCG 
CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG 
CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCAGAGGGCA GGATGGTCTC GGACATCAAC 
AACGCCTGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG 
ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGACAGAA GGCCTCCATC 
CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT 
ACCCTCTCAG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT 
GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC 
TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT 
CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG 
ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCCG CACCCTTCAA CAACTGGATG 
GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATTGTGC ACACCATCGA GGAGATCCAG 
GGACTGACCA CAGCCCATGA GCAGTTCAAA GCCACCCTCC CCGATGCCGA CAAGGAGCGC 
CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC 
ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC 
CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTAA CGGAGGAGCA CGCCCGCCAG 
CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCCCAGG CCAATGTCAT TGGGCCCTGG 
ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG 
GACCAGCTCA ACCACCTGCG GCAATACGAG AAGAGCATCG TCAACTACAA GCCCAAGATC 
GACCAGCTGG AGGGCGACCA CCAGCTGATC CAGGAGGCGC TCATCTTTGA CAACAAGCAC 
ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC 
AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG 
GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG 
CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC 
CCCCAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT TATCTCCATG 
GGTTACAACA TGGGAGAGGC AGAGTTTGCC CGCATCATGA GCATCGTGGA CCCCAACCGC 
CTTGGGGTGG TGACGTTCCA GGCCTTCATC GACTTTATGT CTCGCGAGAC AGCTGATACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CCGGGGATAA GAACTACATC 
ACGGTGGACG AGCTGCGCAG AGAGCTGCCT CCTGACCAGG CTGAGTACTG CATCGCGCGA 
ATGGCCCCCT ACACCGGCCC CGACGCCGTG CCCGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025151088.1
CDS	186..2735
Translation	MLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRAPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQKKTGMMDTDDFRACLISMGYNMGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITVDELRRELPPDQAEYCIARMAPYTGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_025295303.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis actinin alpha 1 (ACTN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025295303.1

ORF Insert Sequence:

ATGCTGCTGC TGGAGGTCAT CTCAGGTGAA CGTTTGGCCA AGCCCGAGAG AGGCAAAATG 
AGGGTGCACA AGATCTCCAA TGTCAACAAG GCCTTGGACT TCATAGCCAG CAAGGGGGTG 
AAGCTGGTGT CCATAGGAGC TGAAGAAATT GTGGATGGGA ATGTGAAAAT GACCCTGGGC 
ATGATCTGGA CCATCATCCT CCGCTTTGCC ATCCAGGACA TCTCCGTGGA AGAGACTTCA 
GCGAAGGAAG GGCTTCTCTT GTGGTGTCAG AGAAAGACAG CCCCTTACAA GAATGTCAAC 
ATCCAGAACT TCCACATAAG CTGGAAGGAC GGCCTCGGCT TCTGTGCCTT GATCCACCGA 
CACCGGCCCG AGCTGATCGA CTACGGGAAG CTGCGGAAGG ATGACCCACT GACAAACTTG 
AACACAGCTT TCGATGTAGC GGAGAAGTAC CTGGACATTC CCAAGATGTT GGATGCGGAA 
GACATCGTCG GAACCGCCCG CCCAGACGAG AAAGCCATCA TGACTTACGT GTCCAGCTTC 
TACCACGCCT TCTCTGGAGC CCAGAAGGCC GAGACAGCAG CCAATCGCAT CTGCAAGGTG 
CTGGCCGTCA ACCAGGAGAA CGAGCAGCTC ATGGAGGACT ACGAGAAGCT GGCCAGCGAC 
CTGCTGGAAT GGATCCGCCG CACCATCCCA TGGCTGGAGA ACCGGGCACC CGAGAACACC 
ATGCACGCCA TGCAGCAGAA GCTGGAGGAC TTCCGGGACT ACCGGCGCCT GCACAAGCCG 
CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG 
CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCAGAGGGCA GGATGGTCTC GGACATCAAC 
AACGCCTGGG GCTGCCTGGA GCAGGCGGAG AAGGGCTATG AGGAGTGGCT GCTGAATGAG 
ATCCGGAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGACAGAA GGCCTCCATC 
CACGAGGCCT GGACGGACGG CAAAGAGGCC ATGCTGCGGC AGAAGGATTA CGAGACGGCT 
ACCCTCTCAG AGATCAAGGC CCTGCTCAAG AAGCACGAGG CCTTTGAGAG TGACCTGGCT 
GCCCACCAGG ACCGCGTGGA GCAGATTGCC GCCATTGCCC AGGAGCTCAA TGAGCTGGAC 
TATTACGACT CACCCAGTGT CAACGCCCGG TGCCAAAAGA TCTGTGACCA GTGGGACAAT 
CTAGGGGCCC TAACCCAGAA GCGAAGGGAA GCTCTGGAGC GCACGGAGAA ACTGCTGGAG 
ACCATTGACC AACTGTACTT GGAGTACGCT AAGCGGGCCG CACCCTTCAA CAACTGGATG 
GAGGGGGCCA TGGAGGACCT GCAGGACACC TTCATTGTGC ACACCATCGA GGAGATCCAG 
GGACTGACCA CAGCCCATGA GCAGTTCAAA GCCACCCTCC CCGATGCCGA CAAGGAGCGC 
CTGGCTATCC TGGGCATCCA CAACGAGGTG TCCAAGATTG TCCAGACCTA CCACGTCAAC 
ATGGCAGGCA CCAACCCCTA CACAACCATC ACGCCTCAGG AGATCAATGG CAAATGGGAC 
CATGTGCGGC AGCTGGTGCC ACGGAGGGAC CAGGCCCTAA CGGAGGAGCA CGCCCGCCAG 
CAGCACAACG AAAGGCTACG CAAGCAGTTT GGGGCCCAGG CCAATGTCAT TGGGCCCTGG 
ATCCAGACCA AGATGGAGGA GATTGGAAGG ATCTCCATTG AGATGCACGG GACCCTGGAG 
GACCAGCTCA ACCACCTGCG GCAATACGAG AAGAGCATCG TCAACTACAA GCCCAAGATC 
GACCAGCTGG AGGGCGACCA CCAGCTGATC CAGGAGGCGC TCATCTTTGA CAACAAGCAC 
ACCAACTACA CCATGGAGCA CATCCGTGTG GGCTGGGAGC AGCTCCTAAC CACCATCGCC 
AGGACCATCA ATGAGGTGGA GAACCAGATC CTGACCAGGG ATGCCAAGGG CATCAGCCAG 
GAGCAGATGA ATGAGTTCCG GGCCTCCTTC AACCACTTTG ACCGGGATCA CTCCGGCACG 
CTGGGTCCCG AGGAGTTCAA AGCCTGCCTC ATCAGCTTGG GTTATGATAT TGGCAACGAC 
CCCCAGAAGA AGACAGGCAT GATGGACACG GATGATTTCC GCGCCTGCCT TATCTCCATG 
GGTTACAACA TGGGAGAGGC AGAGTTTGCC CGCATCATGA GCATCGTGGA CCCCAACCGC 
CTTGGGGTGG TGACGTTCCA GGCCTTCATC GACTTTATGT CTCGCGAGAC AGCTGATACA 
GATACAGCAG ACCAAGTCAT GGCTTCCTTC AAGATCCTGG CCGGGGATAA GAACTACATC 
ACGGTGGACG AGCTGCGCAG AGAGCTGCCT CCTGACCAGG CTGAGTACTG CATCGCGCGA 
ATGGCCCCCT ACACCGGCCC CGACGCCGTG CCCGGTGCTC TGGACTACAT GTCCTTCTCC 
ACGGCGCTGT ACGGCGAGAG TGACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ACTN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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