CUL5 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CUL5
Entrez Gene ID	102397493
Full Name	cullin 5
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006053333.2	XP_006053395.1	cullin-5 isoform X1
XM_006053334.2	XP_006053396.1	cullin-5 isoform X2
XM_006053334.1	XP_006053396.1	cullin-5 isoform X2
XM_025266947.1	XP_025122732.1	cullin-5 isoform X3
XM_006053333.1	XP_006053395.1	cullin-5 isoform X1
XM_025266946.1	XP_025122731.1	cullin-5 isoform X3

Pathways from BioSystems
Gene Ontology

The following CUL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu10930	XM_006053333.2 Latest version!	Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu10931	XM_006053334.2 Latest version!	Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu50898	XM_025266946.1 Latest version!	Bubalus bubalis cullin 5 (CUL5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBu50898	XM_025266947.1 Latest version!	Bubalus bubalis cullin 5 (CUL5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBu10931	XM_006053334.1	Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu10930	XM_006053333.1	Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CUL5 ( XM_006053333.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053395.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu10930
Clone ID Related Accession (Same CDS sequence)	XM_006053333.2 , XM_006053333.1
Accession Version	XM_006053333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTTC AGTTTGAAGA CAAATGGGAT 
TTCATGCGTC CAATTGTTCT GAAGCTTTTA CGCCAGGAAT CTGTTACAAA ACAGCAGTGG 
TTTGATCTGT TTTCGGATGT ACATGCAGTC TGTCTCTGGG ATGATAAAGG CCCAGCAAAA 
ATTCATCAGG CTTTGAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCACGAGTA 
CTGAGCCATC AAGATGATAC AGCTTTGCTA AAAGCTTACA TTGTTGAATG GCGAAAATTC 
TTTACACAGT GTGATATTTT ACCGAAGCCT TTCTGTCAAC TAGAGATTAC TTTAATGGGT 
AAACAGGGCA GCAATAAAAA ATCAAATGTA GAAGACAGTA TTGTTAGAAA GCTCATGCTA 
GATACATGGA ATGAATCAAT CTTTTCAAAT ATAAAAAACA GACTTCAAGA TAGTGCAATG 
AAGCTGGTCC ATGCTGAAAG ATTAGGAGAA GCTTTTGATT CTCAGCTTGT CATTGGAGTA 
AGAGAATCCT ATGTTAACCT TTGTTCTAAT CCAGAGGATA AGCTCCAAAT TTATAGGGAC 
AATTTTGAGA AGGCATACTT GGATTCAACA GAGAGATTTT ATAGAACACA GGCACCCTCG 
TATTTACAAC AAAATGGTGT ACAGAATTAT ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAC GAGCCCTACG TTATTTAGAA ACAAGACGAG AATGTAACTC TGTTGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCCCTGGTG ACATCCTTTA AAGAGACTAT CTTAGCAGAG 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAATTAC ATTTAATGTT TTCATTGATG 
GACAAAGTTC CTAATGGAAT AGAACCAATG TTAAAAGACT TGGAGGAGCA TATCATCAGT 
GCGGGCCTGG CAGATATGGT AGCAGCTGCT GAAACTATTA CTACTGACTC TGAGAAGTAT 
GTTGAGCAGT TACTTACACT ATTTAATAGA TTCAGTAAAC TTGTCAAAGA AGCTTTTCAA 
GATGACCCAC GATTTCTTAC TGCAAGAGAC AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACCATATTTA AACTTGAATT ACCTTTGAAG CAGAAGGGGG TGGGATTAAA AACTCAGCCT 
GAATCAAAAT GCCCTGAGCT GCTTGCCAAT TACTGTGACA TGTTGCTAAG AAAAACGCCA 
TTAAGCAAAA AGCTAACCTC TGAAGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATACG TACAAAACAA AGACGTTTTT ATGAGATATC ACAAAGCTCA TTTGACACGA 
CGTCTTATAT TAGACATCTC TGCTGATAGT GAAATTGAAG AGAATATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTA AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGATTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAACAA TAAATTGGCG 
TTACCAGCTG ATTCAGTTAA TATAAAAATT CTGAATGCTG GTGCCTGGTC AAGAAGCTCT 
GAAAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTCTACAAAA AGAACCATAG TGGAAGAAAA TTACATTGGC ATCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAGAATGA AGTAGGCCAA TATGATTTGG AGGTAACAAC GTTTCAGCTG 
GCTGTATTGT TTGCATGGAA CCAAAGACCC AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACTG AACTCCCTGA TGCTGAGCTT AGGAGGACTT TATGGTCTTT AGTAGCTTTT 
CCAAAGCTCA AACGGCAAGT TTTATTGTAT GAACCTCAAG TCAACTCACC CAAAGACTTT 
ACAGAAGGTA CCCTCTTCTC AGTGAACCAG GAGTTCAGTT TAATAAAAAA TGCAAAAGTT 
CAGAAAAGGG GTAAAATCAA CTTGATTGGA CGCTTGCAGC TCACTACAGA AAGAATGAGA 
GAGGAAGAGA ATGAAGGAAT AGTTCAACTA AGAATACTGA GAACCCAGGA AGCTATCATA 
CAAATAATGA AGATGAGAAA GAAAATTAGT AATGCTCAGC TGCAGACTGA ATTAGTAGAA 
ATTTTGAAAA ACATGTTCCT GCCACAAAAG AAAATGATAA AAGAGCAAAT AGAATGGCTA 
ATAGAGCACA AATACATCAG AAGAGATGAA TCTGATATCA ACACTTTCAT ATATATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053395.1
CDS	438..2780
Translation	MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_006053333.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053333.2

ORF Insert Sequence:

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTTC AGTTTGAAGA CAAATGGGAT 
TTCATGCGTC CAATTGTTCT GAAGCTTTTA CGCCAGGAAT CTGTTACAAA ACAGCAGTGG 
TTTGATCTGT TTTCGGATGT ACATGCAGTC TGTCTCTGGG ATGATAAAGG CCCAGCAAAA 
ATTCATCAGG CTTTGAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCACGAGTA 
CTGAGCCATC AAGATGATAC AGCTTTGCTA AAAGCTTACA TTGTTGAATG GCGAAAATTC 
TTTACACAGT GTGATATTTT ACCGAAGCCT TTCTGTCAAC TAGAGATTAC TTTAATGGGT 
AAACAGGGCA GCAATAAAAA ATCAAATGTA GAAGACAGTA TTGTTAGAAA GCTCATGCTA 
GATACATGGA ATGAATCAAT CTTTTCAAAT ATAAAAAACA GACTTCAAGA TAGTGCAATG 
AAGCTGGTCC ATGCTGAAAG ATTAGGAGAA GCTTTTGATT CTCAGCTTGT CATTGGAGTA 
AGAGAATCCT ATGTTAACCT TTGTTCTAAT CCAGAGGATA AGCTCCAAAT TTATAGGGAC 
AATTTTGAGA AGGCATACTT GGATTCAACA GAGAGATTTT ATAGAACACA GGCACCCTCG 
TATTTACAAC AAAATGGTGT ACAGAATTAT ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAC GAGCCCTACG TTATTTAGAA ACAAGACGAG AATGTAACTC TGTTGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCCCTGGTG ACATCCTTTA AAGAGACTAT CTTAGCAGAG 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAATTAC ATTTAATGTT TTCATTGATG 
GACAAAGTTC CTAATGGAAT AGAACCAATG TTAAAAGACT TGGAGGAGCA TATCATCAGT 
GCGGGCCTGG CAGATATGGT AGCAGCTGCT GAAACTATTA CTACTGACTC TGAGAAGTAT 
GTTGAGCAGT TACTTACACT ATTTAATAGA TTCAGTAAAC TTGTCAAAGA AGCTTTTCAA 
GATGACCCAC GATTTCTTAC TGCAAGAGAC AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACCATATTTA AACTTGAATT ACCTTTGAAG CAGAAGGGGG TGGGATTAAA AACTCAGCCT 
GAATCAAAAT GCCCTGAGCT GCTTGCCAAT TACTGTGACA TGTTGCTAAG AAAAACGCCA 
TTAAGCAAAA AGCTAACCTC TGAAGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATACG TACAAAACAA AGACGTTTTT ATGAGATATC ACAAAGCTCA TTTGACACGA 
CGTCTTATAT TAGACATCTC TGCTGATAGT GAAATTGAAG AGAATATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTA AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGATTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAACAA TAAATTGGCG 
TTACCAGCTG ATTCAGTTAA TATAAAAATT CTGAATGCTG GTGCCTGGTC AAGAAGCTCT 
GAAAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTCTACAAAA AGAACCATAG TGGAAGAAAA TTACATTGGC ATCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAGAATGA AGTAGGCCAA TATGATTTGG AGGTAACAAC GTTTCAGCTG 
GCTGTATTGT TTGCATGGAA CCAAAGACCC AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACTG AACTCCCTGA TGCTGAGCTT AGGAGGACTT TATGGTCTTT AGTAGCTTTT 
CCAAAGCTCA AACGGCAAGT TTTATTGTAT GAACCTCAAG TCAACTCACC CAAAGACTTT 
ACAGAAGGTA CCCTCTTCTC AGTGAACCAG GAGTTCAGTT TAATAAAAAA TGCAAAAGTT 
CAGAAAAGGG GTAAAATCAA CTTGATTGGA CGCTTGCAGC TCACTACAGA AAGAATGAGA 
GAGGAAGAGA ATGAAGGAAT AGTTCAACTA AGAATACTGA GAACCCAGGA AGCTATCATA 
CAAATAATGA AGATGAGAAA GAAAATTAGT AATGCTCAGC TGCAGACTGA ATTAGTAGAA 
ATTTTGAAAA ACATGTTCCT GCCACAAAAG AAAATGATAA AAGAGCAAAT AGAATGGCTA 
ATAGAGCACA AATACATCAG AAGAGATGAA TCTGATATCA ACACTTTCAT ATATATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL5 ( XM_006053333.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053395.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu10930
Clone ID Related Accession (Same CDS sequence)	XM_006053333.2 , XM_006053333.1
Accession Version	XM_006053333.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784514.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTTC AGTTTGAAGA CAAATGGGAT 
TTCATGCGTC CAATTGTTCT GAAGCTTTTA CGCCAGGAAT CTGTTACAAA ACAGCAGTGG 
TTTGATCTGT TTTCGGATGT ACATGCAGTC TGTCTCTGGG ATGATAAAGG CCCAGCAAAA 
ATTCATCAGG CTTTGAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCACGAGTA 
CTGAGCCATC AAGATGATAC AGCTTTGCTA AAAGCTTACA TTGTTGAATG GCGAAAATTC 
TTTACACAGT GTGATATTTT ACCGAAGCCT TTCTGTCAAC TAGAGATTAC TTTAATGGGT 
AAACAGGGCA GCAATAAAAA ATCAAATGTA GAAGACAGTA TTGTTAGAAA GCTCATGCTA 
GATACATGGA ATGAATCAAT CTTTTCAAAT ATAAAAAACA GACTTCAAGA TAGTGCAATG 
AAGCTGGTCC ATGCTGAAAG ATTAGGAGAA GCTTTTGATT CTCAGCTTGT CATTGGAGTA 
AGAGAATCCT ATGTTAACCT TTGTTCTAAT CCAGAGGATA AGCTCCAAAT TTATAGGGAC 
AATTTTGAGA AGGCATACTT GGATTCAACA GAGAGATTTT ATAGAACACA GGCACCCTCG 
TATTTACAAC AAAATGGTGT ACAGAATTAT ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAC GAGCCCTACG TTATTTAGAA ACAAGACGAG AATGTAACTC TGTTGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCCCTGGTG ACATCCTTTA AAGAGACTAT CTTAGCAGAG 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAATTAC ATTTAATGTT TTCATTGATG 
GACAAAGTTC CTAATGGAAT AGAACCAATG TTAAAAGACT TGGAGGAGCA TATCATCAGT 
GCGGGCCTGG CAGATATGGT AGCAGCTGCT GAAACTATTA CTACTGACTC TGAGAAGTAT 
GTTGAGCAGT TACTTACACT ATTTAATAGA TTCAGTAAAC TTGTCAAAGA AGCTTTTCAA 
GATGACCCAC GATTTCTTAC TGCAAGAGAC AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACCATATTTA AACTTGAATT ACCTTTGAAG CAGAAGGGGG TGGGATTAAA AACTCAGCCT 
GAATCAAAAT GCCCTGAGCT GCTTGCCAAT TACTGTGACA TGTTGCTAAG AAAAACGCCA 
TTAAGCAAAA AGCTAACCTC TGAAGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATACG TACAAAACAA AGACGTTTTT ATGAGATATC ACAAAGCTCA TTTGACACGA 
CGTCTTATAT TAGACATCTC TGCTGATAGT GAAATTGAAG AGAATATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTA AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGATTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAACAA TAAATTGGCG 
TTACCAGCTG ATTCAGTTAA TATAAAAATT CTGAATGCTG GTGCCTGGTC AAGAAGCTCT 
GAAAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTCTACAAAA AGAACCATAG TGGAAGAAAA TTACATTGGC ATCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAGAATGA AGTAGGCCAA TATGATTTGG AGGTAACAAC GTTTCAGCTG 
GCTGTATTGT TTGCATGGAA CCAAAGACCC AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACTG AACTCCCTGA TGCTGAGCTT AGGAGGACTT TATGGTCTTT AGTAGCTTTT 
CCAAAGCTCA AACGGCAAGT TTTATTGTAT GAACCTCAAG TCAACTCACC CAAAGACTTT 
ACAGAAGGTA CCCTCTTCTC AGTGAACCAG GAGTTCAGTT TAATAAAAAA TGCAAAAGTT 
CAGAAAAGGG GTAAAATCAA CTTGATTGGA CGCTTGCAGC TCACTACAGA AAGAATGAGA 
GAGGAAGAGA ATGAAGGAAT AGTTCAACTA AGAATACTGA GAACCCAGGA AGCTATCATA 
CAAATAATGA AGATGAGAAA GAAAATTAGT AATGCTCAGC TGCAGACTGA ATTAGTAGAA 
ATTTTGAAAA ACATGTTCCT GCCACAAAAG AAAATGATAA AAGAGCAAAT AGAATGGCTA 
ATAGAGCACA AATACATCAG AAGAGATGAA TCTGATATCA ACACTTTCAT ATATATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053395.1
CDS	435..2777
Translation	MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_006053333.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053333.1

ORF Insert Sequence:

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTTC AGTTTGAAGA CAAATGGGAT 
TTCATGCGTC CAATTGTTCT GAAGCTTTTA CGCCAGGAAT CTGTTACAAA ACAGCAGTGG 
TTTGATCTGT TTTCGGATGT ACATGCAGTC TGTCTCTGGG ATGATAAAGG CCCAGCAAAA 
ATTCATCAGG CTTTGAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCACGAGTA 
CTGAGCCATC AAGATGATAC AGCTTTGCTA AAAGCTTACA TTGTTGAATG GCGAAAATTC 
TTTACACAGT GTGATATTTT ACCGAAGCCT TTCTGTCAAC TAGAGATTAC TTTAATGGGT 
AAACAGGGCA GCAATAAAAA ATCAAATGTA GAAGACAGTA TTGTTAGAAA GCTCATGCTA 
GATACATGGA ATGAATCAAT CTTTTCAAAT ATAAAAAACA GACTTCAAGA TAGTGCAATG 
AAGCTGGTCC ATGCTGAAAG ATTAGGAGAA GCTTTTGATT CTCAGCTTGT CATTGGAGTA 
AGAGAATCCT ATGTTAACCT TTGTTCTAAT CCAGAGGATA AGCTCCAAAT TTATAGGGAC 
AATTTTGAGA AGGCATACTT GGATTCAACA GAGAGATTTT ATAGAACACA GGCACCCTCG 
TATTTACAAC AAAATGGTGT ACAGAATTAT ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAC GAGCCCTACG TTATTTAGAA ACAAGACGAG AATGTAACTC TGTTGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCCCTGGTG ACATCCTTTA AAGAGACTAT CTTAGCAGAG 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAATTAC ATTTAATGTT TTCATTGATG 
GACAAAGTTC CTAATGGAAT AGAACCAATG TTAAAAGACT TGGAGGAGCA TATCATCAGT 
GCGGGCCTGG CAGATATGGT AGCAGCTGCT GAAACTATTA CTACTGACTC TGAGAAGTAT 
GTTGAGCAGT TACTTACACT ATTTAATAGA TTCAGTAAAC TTGTCAAAGA AGCTTTTCAA 
GATGACCCAC GATTTCTTAC TGCAAGAGAC AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACCATATTTA AACTTGAATT ACCTTTGAAG CAGAAGGGGG TGGGATTAAA AACTCAGCCT 
GAATCAAAAT GCCCTGAGCT GCTTGCCAAT TACTGTGACA TGTTGCTAAG AAAAACGCCA 
TTAAGCAAAA AGCTAACCTC TGAAGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATACG TACAAAACAA AGACGTTTTT ATGAGATATC ACAAAGCTCA TTTGACACGA 
CGTCTTATAT TAGACATCTC TGCTGATAGT GAAATTGAAG AGAATATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTA AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGATTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAACAA TAAATTGGCG 
TTACCAGCTG ATTCAGTTAA TATAAAAATT CTGAATGCTG GTGCCTGGTC AAGAAGCTCT 
GAAAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTCTACAAAA AGAACCATAG TGGAAGAAAA TTACATTGGC ATCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAGAATGA AGTAGGCCAA TATGATTTGG AGGTAACAAC GTTTCAGCTG 
GCTGTATTGT TTGCATGGAA CCAAAGACCC AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACTG AACTCCCTGA TGCTGAGCTT AGGAGGACTT TATGGTCTTT AGTAGCTTTT 
CCAAAGCTCA AACGGCAAGT TTTATTGTAT GAACCTCAAG TCAACTCACC CAAAGACTTT 
ACAGAAGGTA CCCTCTTCTC AGTGAACCAG GAGTTCAGTT TAATAAAAAA TGCAAAAGTT 
CAGAAAAGGG GTAAAATCAA CTTGATTGGA CGCTTGCAGC TCACTACAGA AAGAATGAGA 
GAGGAAGAGA ATGAAGGAAT AGTTCAACTA AGAATACTGA GAACCCAGGA AGCTATCATA 
CAAATAATGA AGATGAGAAA GAAAATTAGT AATGCTCAGC TGCAGACTGA ATTAGTAGAA 
ATTTTGAAAA ACATGTTCCT GCCACAAAAG AAAATGATAA AAGAGCAAAT AGAATGGCTA 
ATAGAGCACA AATACATCAG AAGAGATGAA TCTGATATCA ACACTTTCAT ATATATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL5 ( XM_006053334.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053396.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu10931
Clone ID Related Accession (Same CDS sequence)	XM_006053334.2 , XM_006053334.1
Accession Version	XM_006053334.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCCAA TTGTTCTGAA GCTTTTACGC CAGGAATCTG TTACAAAACA GCAGTGGTTT 
GATCTGTTTT CGGATGTACA TGCAGTCTGT CTCTGGGATG ATAAAGGCCC AGCAAAAATT 
CATCAGGCTT TGAAAGAAGA TATTCTTGAG TTTATTAAGC AAGCACAGGC ACGAGTACTG 
AGCCATCAAG ATGATACAGC TTTGCTAAAA GCTTACATTG TTGAATGGCG AAAATTCTTT 
ACACAGTGTG ATATTTTACC GAAGCCTTTC TGTCAACTAG AGATTACTTT AATGGGTAAA 
CAGGGCAGCA ATAAAAAATC AAATGTAGAA GACAGTATTG TTAGAAAGCT CATGCTAGAT 
ACATGGAATG AATCAATCTT TTCAAATATA AAAAACAGAC TTCAAGATAG TGCAATGAAG 
CTGGTCCATG CTGAAAGATT AGGAGAAGCT TTTGATTCTC AGCTTGTCAT TGGAGTAAGA 
GAATCCTATG TTAACCTTTG TTCTAATCCA GAGGATAAGC TCCAAATTTA TAGGGACAAT 
TTTGAGAAGG CATACTTGGA TTCAACAGAG AGATTTTATA GAACACAGGC ACCCTCGTAT 
TTACAACAAA ATGGTGTACA GAATTATATG AAATATGCAG ATGCTAAATT AAAAGAAGAA 
GAAAAACGAG CCCTACGTTA TTTAGAAACA AGACGAGAAT GTAACTCTGT TGAAGCACTC 
ATGGAATGCT GTGTAAATGC CCTGGTGACA TCCTTTAAAG AGACTATCTT AGCAGAGTGC 
CAAGGCATGA TCAAGCGAAA TGAAACTGAA AAATTACATT TAATGTTTTC ATTGATGGAC 
AAAGTTCCTA ATGGAATAGA ACCAATGTTA AAAGACTTGG AGGAGCATAT CATCAGTGCG 
GGCCTGGCAG ATATGGTAGC AGCTGCTGAA ACTATTACTA CTGACTCTGA GAAGTATGTT 
GAGCAGTTAC TTACACTATT TAATAGATTC AGTAAACTTG TCAAAGAAGC TTTTCAAGAT 
GACCCACGAT TTCTTACTGC AAGAGACAAG GCATATAAAG CAGTTGTTAA TGATGCTACC 
ATATTTAAAC TTGAATTACC TTTGAAGCAG AAGGGGGTGG GATTAAAAAC TCAGCCTGAA 
TCAAAATGCC CTGAGCTGCT TGCCAATTAC TGTGACATGT TGCTAAGAAA AACGCCATTA 
AGCAAAAAGC TAACCTCTGA AGAGATTGAA GCAAAGCTTA AAGAAGTGCT CTTGGTACTT 
AAATACGTAC AAAACAAAGA CGTTTTTATG AGATATCACA AAGCTCATTT GACACGACGT 
CTTATATTAG ACATCTCTGC TGATAGTGAA ATTGAAGAGA ATATGGTAGA GTGGCTAAGA 
GAAGTTGGTA TGCCAGCAGA TTATGTAAAC AAGCTTGCTA GAATGTTTCA GGACATAAAA 
GTATCTGAAG ATTTGAACCA AGCTTTTAAG GAAATGCACA AAAACAATAA ATTGGCGTTA 
CCAGCTGATT CAGTTAATAT AAAAATTCTG AATGCTGGTG CCTGGTCAAG AAGCTCTGAA 
AAAGTCTTTG TCTCACTTCC TACTGAACTG GAGGATTTGA TACCTGAAGT AGAAGAATTC 
TACAAAAAGA ACCATAGTGG AAGAAAATTA CATTGGCATC ATCTCATGTC AAATGGAATT 
ATAACATTTA AGAATGAAGT AGGCCAATAT GATTTGGAGG TAACAACGTT TCAGCTGGCT 
GTATTGTTTG CATGGAACCA AAGACCCAGA GAGAAAATCA GCTTTGAAAA TCTAAAACTT 
GCAACTGAAC TCCCTGATGC TGAGCTTAGG AGGACTTTAT GGTCTTTAGT AGCTTTTCCA 
AAGCTCAAAC GGCAAGTTTT ATTGTATGAA CCTCAAGTCA ACTCACCCAA AGACTTTACA 
GAAGGTACCC TCTTCTCAGT GAACCAGGAG TTCAGTTTAA TAAAAAATGC AAAAGTTCAG 
AAAAGGGGTA AAATCAACTT GATTGGACGC TTGCAGCTCA CTACAGAAAG AATGAGAGAG 
GAAGAGAATG AAGGAATAGT TCAACTAAGA ATACTGAGAA CCCAGGAAGC TATCATACAA 
ATAATGAAGA TGAGAAAGAA AATTAGTAAT GCTCAGCTGC AGACTGAATT AGTAGAAATT 
TTGAAAAACA TGTTCCTGCC ACAAAAGAAA ATGATAAAAG AGCAAATAGA ATGGCTAATA 
GAGCACAAAT ACATCAGAAG AGATGAATCT GATATCAACA CTTTCATATA TATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053396.1
CDS	550..2829
Translation	MRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_006053334.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053334.2

ORF Insert Sequence:

ATGCGTCCAA TTGTTCTGAA GCTTTTACGC CAGGAATCTG TTACAAAACA GCAGTGGTTT 
GATCTGTTTT CGGATGTACA TGCAGTCTGT CTCTGGGATG ATAAAGGCCC AGCAAAAATT 
CATCAGGCTT TGAAAGAAGA TATTCTTGAG TTTATTAAGC AAGCACAGGC ACGAGTACTG 
AGCCATCAAG ATGATACAGC TTTGCTAAAA GCTTACATTG TTGAATGGCG AAAATTCTTT 
ACACAGTGTG ATATTTTACC GAAGCCTTTC TGTCAACTAG AGATTACTTT AATGGGTAAA 
CAGGGCAGCA ATAAAAAATC AAATGTAGAA GACAGTATTG TTAGAAAGCT CATGCTAGAT 
ACATGGAATG AATCAATCTT TTCAAATATA AAAAACAGAC TTCAAGATAG TGCAATGAAG 
CTGGTCCATG CTGAAAGATT AGGAGAAGCT TTTGATTCTC AGCTTGTCAT TGGAGTAAGA 
GAATCCTATG TTAACCTTTG TTCTAATCCA GAGGATAAGC TCCAAATTTA TAGGGACAAT 
TTTGAGAAGG CATACTTGGA TTCAACAGAG AGATTTTATA GAACACAGGC ACCCTCGTAT 
TTACAACAAA ATGGTGTACA GAATTATATG AAATATGCAG ATGCTAAATT AAAAGAAGAA 
GAAAAACGAG CCCTACGTTA TTTAGAAACA AGACGAGAAT GTAACTCTGT TGAAGCACTC 
ATGGAATGCT GTGTAAATGC CCTGGTGACA TCCTTTAAAG AGACTATCTT AGCAGAGTGC 
CAAGGCATGA TCAAGCGAAA TGAAACTGAA AAATTACATT TAATGTTTTC ATTGATGGAC 
AAAGTTCCTA ATGGAATAGA ACCAATGTTA AAAGACTTGG AGGAGCATAT CATCAGTGCG 
GGCCTGGCAG ATATGGTAGC AGCTGCTGAA ACTATTACTA CTGACTCTGA GAAGTATGTT 
GAGCAGTTAC TTACACTATT TAATAGATTC AGTAAACTTG TCAAAGAAGC TTTTCAAGAT 
GACCCACGAT TTCTTACTGC AAGAGACAAG GCATATAAAG CAGTTGTTAA TGATGCTACC 
ATATTTAAAC TTGAATTACC TTTGAAGCAG AAGGGGGTGG GATTAAAAAC TCAGCCTGAA 
TCAAAATGCC CTGAGCTGCT TGCCAATTAC TGTGACATGT TGCTAAGAAA AACGCCATTA 
AGCAAAAAGC TAACCTCTGA AGAGATTGAA GCAAAGCTTA AAGAAGTGCT CTTGGTACTT 
AAATACGTAC AAAACAAAGA CGTTTTTATG AGATATCACA AAGCTCATTT GACACGACGT 
CTTATATTAG ACATCTCTGC TGATAGTGAA ATTGAAGAGA ATATGGTAGA GTGGCTAAGA 
GAAGTTGGTA TGCCAGCAGA TTATGTAAAC AAGCTTGCTA GAATGTTTCA GGACATAAAA 
GTATCTGAAG ATTTGAACCA AGCTTTTAAG GAAATGCACA AAAACAATAA ATTGGCGTTA 
CCAGCTGATT CAGTTAATAT AAAAATTCTG AATGCTGGTG CCTGGTCAAG AAGCTCTGAA 
AAAGTCTTTG TCTCACTTCC TACTGAACTG GAGGATTTGA TACCTGAAGT AGAAGAATTC 
TACAAAAAGA ACCATAGTGG AAGAAAATTA CATTGGCATC ATCTCATGTC AAATGGAATT 
ATAACATTTA AGAATGAAGT AGGCCAATAT GATTTGGAGG TAACAACGTT TCAGCTGGCT 
GTATTGTTTG CATGGAACCA AAGACCCAGA GAGAAAATCA GCTTTGAAAA TCTAAAACTT 
GCAACTGAAC TCCCTGATGC TGAGCTTAGG AGGACTTTAT GGTCTTTAGT AGCTTTTCCA 
AAGCTCAAAC GGCAAGTTTT ATTGTATGAA CCTCAAGTCA ACTCACCCAA AGACTTTACA 
GAAGGTACCC TCTTCTCAGT GAACCAGGAG TTCAGTTTAA TAAAAAATGC AAAAGTTCAG 
AAAAGGGGTA AAATCAACTT GATTGGACGC TTGCAGCTCA CTACAGAAAG AATGAGAGAG 
GAAGAGAATG AAGGAATAGT TCAACTAAGA ATACTGAGAA CCCAGGAAGC TATCATACAA 
ATAATGAAGA TGAGAAAGAA AATTAGTAAT GCTCAGCTGC AGACTGAATT AGTAGAAATT 
TTGAAAAACA TGTTCCTGCC ACAAAAGAAA ATGATAAAAG AGCAAATAGA ATGGCTAATA 
GAGCACAAAT ACATCAGAAG AGATGAATCT GATATCAACA CTTTCATATA TATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL5 ( XM_006053334.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053396.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.

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CloneID	OBu10931
Clone ID Related Accession (Same CDS sequence)	XM_006053334.2 , XM_006053334.1
Accession Version	XM_006053334.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784514.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCCAA TTGTTCTGAA GCTTTTACGC CAGGAATCTG TTACAAAACA GCAGTGGTTT 
GATCTGTTTT CGGATGTACA TGCAGTCTGT CTCTGGGATG ATAAAGGCCC AGCAAAAATT 
CATCAGGCTT TGAAAGAAGA TATTCTTGAG TTTATTAAGC AAGCACAGGC ACGAGTACTG 
AGCCATCAAG ATGATACAGC TTTGCTAAAA GCTTACATTG TTGAATGGCG AAAATTCTTT 
ACACAGTGTG ATATTTTACC GAAGCCTTTC TGTCAACTAG AGATTACTTT AATGGGTAAA 
CAGGGCAGCA ATAAAAAATC AAATGTAGAA GACAGTATTG TTAGAAAGCT CATGCTAGAT 
ACATGGAATG AATCAATCTT TTCAAATATA AAAAACAGAC TTCAAGATAG TGCAATGAAG 
CTGGTCCATG CTGAAAGATT AGGAGAAGCT TTTGATTCTC AGCTTGTCAT TGGAGTAAGA 
GAATCCTATG TTAACCTTTG TTCTAATCCA GAGGATAAGC TCCAAATTTA TAGGGACAAT 
TTTGAGAAGG CATACTTGGA TTCAACAGAG AGATTTTATA GAACACAGGC ACCCTCGTAT 
TTACAACAAA ATGGTGTACA GAATTATATG AAATATGCAG ATGCTAAATT AAAAGAAGAA 
GAAAAACGAG CCCTACGTTA TTTAGAAACA AGACGAGAAT GTAACTCTGT TGAAGCACTC 
ATGGAATGCT GTGTAAATGC CCTGGTGACA TCCTTTAAAG AGACTATCTT AGCAGAGTGC 
CAAGGCATGA TCAAGCGAAA TGAAACTGAA AAATTACATT TAATGTTTTC ATTGATGGAC 
AAAGTTCCTA ATGGAATAGA ACCAATGTTA AAAGACTTGG AGGAGCATAT CATCAGTGCG 
GGCCTGGCAG ATATGGTAGC AGCTGCTGAA ACTATTACTA CTGACTCTGA GAAGTATGTT 
GAGCAGTTAC TTACACTATT TAATAGATTC AGTAAACTTG TCAAAGAAGC TTTTCAAGAT 
GACCCACGAT TTCTTACTGC AAGAGACAAG GCATATAAAG CAGTTGTTAA TGATGCTACC 
ATATTTAAAC TTGAATTACC TTTGAAGCAG AAGGGGGTGG GATTAAAAAC TCAGCCTGAA 
TCAAAATGCC CTGAGCTGCT TGCCAATTAC TGTGACATGT TGCTAAGAAA AACGCCATTA 
AGCAAAAAGC TAACCTCTGA AGAGATTGAA GCAAAGCTTA AAGAAGTGCT CTTGGTACTT 
AAATACGTAC AAAACAAAGA CGTTTTTATG AGATATCACA AAGCTCATTT GACACGACGT 
CTTATATTAG ACATCTCTGC TGATAGTGAA ATTGAAGAGA ATATGGTAGA GTGGCTAAGA 
GAAGTTGGTA TGCCAGCAGA TTATGTAAAC AAGCTTGCTA GAATGTTTCA GGACATAAAA 
GTATCTGAAG ATTTGAACCA AGCTTTTAAG GAAATGCACA AAAACAATAA ATTGGCGTTA 
CCAGCTGATT CAGTTAATAT AAAAATTCTG AATGCTGGTG CCTGGTCAAG AAGCTCTGAA 
AAAGTCTTTG TCTCACTTCC TACTGAACTG GAGGATTTGA TACCTGAAGT AGAAGAATTC 
TACAAAAAGA ACCATAGTGG AAGAAAATTA CATTGGCATC ATCTCATGTC AAATGGAATT 
ATAACATTTA AGAATGAAGT AGGCCAATAT GATTTGGAGG TAACAACGTT TCAGCTGGCT 
GTATTGTTTG CATGGAACCA AAGACCCAGA GAGAAAATCA GCTTTGAAAA TCTAAAACTT 
GCAACTGAAC TCCCTGATGC TGAGCTTAGG AGGACTTTAT GGTCTTTAGT AGCTTTTCCA 
AAGCTCAAAC GGCAAGTTTT ATTGTATGAA CCTCAAGTCA ACTCACCCAA AGACTTTACA 
GAAGGTACCC TCTTCTCAGT GAACCAGGAG TTCAGTTTAA TAAAAAATGC AAAAGTTCAG 
AAAAGGGGTA AAATCAACTT GATTGGACGC TTGCAGCTCA CTACAGAAAG AATGAGAGAG 
GAAGAGAATG AAGGAATAGT TCAACTAAGA ATACTGAGAA CCCAGGAAGC TATCATACAA 
ATAATGAAGA TGAGAAAGAA AATTAGTAAT GCTCAGCTGC AGACTGAATT AGTAGAAATT 
TTGAAAAACA TGTTCCTGCC ACAAAAGAAA ATGATAAAAG AGCAAATAGA ATGGCTAATA 
GAGCACAAAT ACATCAGAAG AGATGAATCT GATATCAACA CTTTCATATA TATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053396.1
CDS	548..2827
Translation	MRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_006053334.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053334.1

ORF Insert Sequence:

ATGCGTCCAA TTGTTCTGAA GCTTTTACGC CAGGAATCTG TTACAAAACA GCAGTGGTTT 
GATCTGTTTT CGGATGTACA TGCAGTCTGT CTCTGGGATG ATAAAGGCCC AGCAAAAATT 
CATCAGGCTT TGAAAGAAGA TATTCTTGAG TTTATTAAGC AAGCACAGGC ACGAGTACTG 
AGCCATCAAG ATGATACAGC TTTGCTAAAA GCTTACATTG TTGAATGGCG AAAATTCTTT 
ACACAGTGTG ATATTTTACC GAAGCCTTTC TGTCAACTAG AGATTACTTT AATGGGTAAA 
CAGGGCAGCA ATAAAAAATC AAATGTAGAA GACAGTATTG TTAGAAAGCT CATGCTAGAT 
ACATGGAATG AATCAATCTT TTCAAATATA AAAAACAGAC TTCAAGATAG TGCAATGAAG 
CTGGTCCATG CTGAAAGATT AGGAGAAGCT TTTGATTCTC AGCTTGTCAT TGGAGTAAGA 
GAATCCTATG TTAACCTTTG TTCTAATCCA GAGGATAAGC TCCAAATTTA TAGGGACAAT 
TTTGAGAAGG CATACTTGGA TTCAACAGAG AGATTTTATA GAACACAGGC ACCCTCGTAT 
TTACAACAAA ATGGTGTACA GAATTATATG AAATATGCAG ATGCTAAATT AAAAGAAGAA 
GAAAAACGAG CCCTACGTTA TTTAGAAACA AGACGAGAAT GTAACTCTGT TGAAGCACTC 
ATGGAATGCT GTGTAAATGC CCTGGTGACA TCCTTTAAAG AGACTATCTT AGCAGAGTGC 
CAAGGCATGA TCAAGCGAAA TGAAACTGAA AAATTACATT TAATGTTTTC ATTGATGGAC 
AAAGTTCCTA ATGGAATAGA ACCAATGTTA AAAGACTTGG AGGAGCATAT CATCAGTGCG 
GGCCTGGCAG ATATGGTAGC AGCTGCTGAA ACTATTACTA CTGACTCTGA GAAGTATGTT 
GAGCAGTTAC TTACACTATT TAATAGATTC AGTAAACTTG TCAAAGAAGC TTTTCAAGAT 
GACCCACGAT TTCTTACTGC AAGAGACAAG GCATATAAAG CAGTTGTTAA TGATGCTACC 
ATATTTAAAC TTGAATTACC TTTGAAGCAG AAGGGGGTGG GATTAAAAAC TCAGCCTGAA 
TCAAAATGCC CTGAGCTGCT TGCCAATTAC TGTGACATGT TGCTAAGAAA AACGCCATTA 
AGCAAAAAGC TAACCTCTGA AGAGATTGAA GCAAAGCTTA AAGAAGTGCT CTTGGTACTT 
AAATACGTAC AAAACAAAGA CGTTTTTATG AGATATCACA AAGCTCATTT GACACGACGT 
CTTATATTAG ACATCTCTGC TGATAGTGAA ATTGAAGAGA ATATGGTAGA GTGGCTAAGA 
GAAGTTGGTA TGCCAGCAGA TTATGTAAAC AAGCTTGCTA GAATGTTTCA GGACATAAAA 
GTATCTGAAG ATTTGAACCA AGCTTTTAAG GAAATGCACA AAAACAATAA ATTGGCGTTA 
CCAGCTGATT CAGTTAATAT AAAAATTCTG AATGCTGGTG CCTGGTCAAG AAGCTCTGAA 
AAAGTCTTTG TCTCACTTCC TACTGAACTG GAGGATTTGA TACCTGAAGT AGAAGAATTC 
TACAAAAAGA ACCATAGTGG AAGAAAATTA CATTGGCATC ATCTCATGTC AAATGGAATT 
ATAACATTTA AGAATGAAGT AGGCCAATAT GATTTGGAGG TAACAACGTT TCAGCTGGCT 
GTATTGTTTG CATGGAACCA AAGACCCAGA GAGAAAATCA GCTTTGAAAA TCTAAAACTT 
GCAACTGAAC TCCCTGATGC TGAGCTTAGG AGGACTTTAT GGTCTTTAGT AGCTTTTCCA 
AAGCTCAAAC GGCAAGTTTT ATTGTATGAA CCTCAAGTCA ACTCACCCAA AGACTTTACA 
GAAGGTACCC TCTTCTCAGT GAACCAGGAG TTCAGTTTAA TAAAAAATGC AAAAGTTCAG 
AAAAGGGGTA AAATCAACTT GATTGGACGC TTGCAGCTCA CTACAGAAAG AATGAGAGAG 
GAAGAGAATG AAGGAATAGT TCAACTAAGA ATACTGAGAA CCCAGGAAGC TATCATACAA 
ATAATGAAGA TGAGAAAGAA AATTAGTAAT GCTCAGCTGC AGACTGAATT AGTAGAAATT 
TTGAAAAACA TGTTCCTGCC ACAAAAGAAA ATGATAAAAG AGCAAATAGA ATGGCTAATA 
GAGCACAAAT ACATCAGAAG AGATGAATCT GATATCAACA CTTTCATATA TATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL5 ( XM_025266947.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122732.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X4, mRNA.

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CloneID	OBu50898
Clone ID Related Accession (Same CDS sequence)	XM_025266947.1 , XM_025266946.1
Accession Version	XM_025266947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAC AGGGCAGCAA TAAAAAATCA AATGTAGAAG ACAGTATTGT TAGAAAGCTC 
ATGCTAGATA CATGGAATGA ATCAATCTTT TCAAATATAA AAAACAGACT TCAAGATAGT 
GCAATGAAGC TGGTCCATGC TGAAAGATTA GGAGAAGCTT TTGATTCTCA GCTTGTCATT 
GGAGTAAGAG AATCCTATGT TAACCTTTGT TCTAATCCAG AGGATAAGCT CCAAATTTAT 
AGGGACAATT TTGAGAAGGC ATACTTGGAT TCAACAGAGA GATTTTATAG AACACAGGCA 
CCCTCGTATT TACAACAAAA TGGTGTACAG AATTATATGA AATATGCAGA TGCTAAATTA 
AAAGAAGAAG AAAAACGAGC CCTACGTTAT TTAGAAACAA GACGAGAATG TAACTCTGTT 
GAAGCACTCA TGGAATGCTG TGTAAATGCC CTGGTGACAT CCTTTAAAGA GACTATCTTA 
GCAGAGTGCC AAGGCATGAT CAAGCGAAAT GAAACTGAAA AATTACATTT AATGTTTTCA 
TTGATGGACA AAGTTCCTAA TGGAATAGAA CCAATGTTAA AAGACTTGGA GGAGCATATC 
ATCAGTGCGG GCCTGGCAGA TATGGTAGCA GCTGCTGAAA CTATTACTAC TGACTCTGAG 
AAGTATGTTG AGCAGTTACT TACACTATTT AATAGATTCA GTAAACTTGT CAAAGAAGCT 
TTTCAAGATG ACCCACGATT TCTTACTGCA AGAGACAAGG CATATAAAGC AGTTGTTAAT 
GATGCTACCA TATTTAAACT TGAATTACCT TTGAAGCAGA AGGGGGTGGG ATTAAAAACT 
CAGCCTGAAT CAAAATGCCC TGAGCTGCTT GCCAATTACT GTGACATGTT GCTAAGAAAA 
ACGCCATTAA GCAAAAAGCT AACCTCTGAA GAGATTGAAG CAAAGCTTAA AGAAGTGCTC 
TTGGTACTTA AATACGTACA AAACAAAGAC GTTTTTATGA GATATCACAA AGCTCATTTG 
ACACGACGTC TTATATTAGA CATCTCTGCT GATAGTGAAA TTGAAGAGAA TATGGTAGAG 
TGGCTAAGAG AAGTTGGTAT GCCAGCAGAT TATGTAAACA AGCTTGCTAG AATGTTTCAG 
GACATAAAAG TATCTGAAGA TTTGAACCAA GCTTTTAAGG AAATGCACAA AAACAATAAA 
TTGGCGTTAC CAGCTGATTC AGTTAATATA AAAATTCTGA ATGCTGGTGC CTGGTCAAGA 
AGCTCTGAAA AAGTCTTTGT CTCACTTCCT ACTGAACTGG AGGATTTGAT ACCTGAAGTA 
GAAGAATTCT ACAAAAAGAA CCATAGTGGA AGAAAATTAC ATTGGCATCA TCTCATGTCA 
AATGGAATTA TAACATTTAA GAATGAAGTA GGCCAATATG ATTTGGAGGT AACAACGTTT 
CAGCTGGCTG TATTGTTTGC ATGGAACCAA AGACCCAGAG AGAAAATCAG CTTTGAAAAT 
CTAAAACTTG CAACTGAACT CCCTGATGCT GAGCTTAGGA GGACTTTATG GTCTTTAGTA 
GCTTTTCCAA AGCTCAAACG GCAAGTTTTA TTGTATGAAC CTCAAGTCAA CTCACCCAAA 
GACTTTACAG AAGGTACCCT CTTCTCAGTG AACCAGGAGT TCAGTTTAAT AAAAAATGCA 
AAAGTTCAGA AAAGGGGTAA AATCAACTTG ATTGGACGCT TGCAGCTCAC TACAGAAAGA 
ATGAGAGAGG AAGAGAATGA AGGAATAGTT CAACTAAGAA TACTGAGAAC CCAGGAAGCT 
ATCATACAAA TAATGAAGAT GAGAAAGAAA ATTAGTAATG CTCAGCTGCA GACTGAATTA 
GTAGAAATTT TGAAAAACAT GTTCCTGCCA CAAAAGAAAA TGATAAAAGA GCAAATAGAA 
TGGCTAATAG AGCACAAATA CATCAGAAGA GATGAATCTG ATATCAACAC TTTCATATAT 
ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122732.1
CDS	205..2193
Translation	MGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_025266947.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266947.1

ORF Insert Sequence:

ATGGGTAAAC AGGGCAGCAA TAAAAAATCA AATGTAGAAG ACAGTATTGT TAGAAAGCTC 
ATGCTAGATA CATGGAATGA ATCAATCTTT TCAAATATAA AAAACAGACT TCAAGATAGT 
GCAATGAAGC TGGTCCATGC TGAAAGATTA GGAGAAGCTT TTGATTCTCA GCTTGTCATT 
GGAGTAAGAG AATCCTATGT TAACCTTTGT TCTAATCCAG AGGATAAGCT CCAAATTTAT 
AGGGACAATT TTGAGAAGGC ATACTTGGAT TCAACAGAGA GATTTTATAG AACACAGGCA 
CCCTCGTATT TACAACAAAA TGGTGTACAG AATTATATGA AATATGCAGA TGCTAAATTA 
AAAGAAGAAG AAAAACGAGC CCTACGTTAT TTAGAAACAA GACGAGAATG TAACTCTGTT 
GAAGCACTCA TGGAATGCTG TGTAAATGCC CTGGTGACAT CCTTTAAAGA GACTATCTTA 
GCAGAGTGCC AAGGCATGAT CAAGCGAAAT GAAACTGAAA AATTACATTT AATGTTTTCA 
TTGATGGACA AAGTTCCTAA TGGAATAGAA CCAATGTTAA AAGACTTGGA GGAGCATATC 
ATCAGTGCGG GCCTGGCAGA TATGGTAGCA GCTGCTGAAA CTATTACTAC TGACTCTGAG 
AAGTATGTTG AGCAGTTACT TACACTATTT AATAGATTCA GTAAACTTGT CAAAGAAGCT 
TTTCAAGATG ACCCACGATT TCTTACTGCA AGAGACAAGG CATATAAAGC AGTTGTTAAT 
GATGCTACCA TATTTAAACT TGAATTACCT TTGAAGCAGA AGGGGGTGGG ATTAAAAACT 
CAGCCTGAAT CAAAATGCCC TGAGCTGCTT GCCAATTACT GTGACATGTT GCTAAGAAAA 
ACGCCATTAA GCAAAAAGCT AACCTCTGAA GAGATTGAAG CAAAGCTTAA AGAAGTGCTC 
TTGGTACTTA AATACGTACA AAACAAAGAC GTTTTTATGA GATATCACAA AGCTCATTTG 
ACACGACGTC TTATATTAGA CATCTCTGCT GATAGTGAAA TTGAAGAGAA TATGGTAGAG 
TGGCTAAGAG AAGTTGGTAT GCCAGCAGAT TATGTAAACA AGCTTGCTAG AATGTTTCAG 
GACATAAAAG TATCTGAAGA TTTGAACCAA GCTTTTAAGG AAATGCACAA AAACAATAAA 
TTGGCGTTAC CAGCTGATTC AGTTAATATA AAAATTCTGA ATGCTGGTGC CTGGTCAAGA 
AGCTCTGAAA AAGTCTTTGT CTCACTTCCT ACTGAACTGG AGGATTTGAT ACCTGAAGTA 
GAAGAATTCT ACAAAAAGAA CCATAGTGGA AGAAAATTAC ATTGGCATCA TCTCATGTCA 
AATGGAATTA TAACATTTAA GAATGAAGTA GGCCAATATG ATTTGGAGGT AACAACGTTT 
CAGCTGGCTG TATTGTTTGC ATGGAACCAA AGACCCAGAG AGAAAATCAG CTTTGAAAAT 
CTAAAACTTG CAACTGAACT CCCTGATGCT GAGCTTAGGA GGACTTTATG GTCTTTAGTA 
GCTTTTCCAA AGCTCAAACG GCAAGTTTTA TTGTATGAAC CTCAAGTCAA CTCACCCAAA 
GACTTTACAG AAGGTACCCT CTTCTCAGTG AACCAGGAGT TCAGTTTAAT AAAAAATGCA 
AAAGTTCAGA AAAGGGGTAA AATCAACTTG ATTGGACGCT TGCAGCTCAC TACAGAAAGA 
ATGAGAGAGG AAGAGAATGA AGGAATAGTT CAACTAAGAA TACTGAGAAC CCAGGAAGCT 
ATCATACAAA TAATGAAGAT GAGAAAGAAA ATTAGTAATG CTCAGCTGCA GACTGAATTA 
GTAGAAATTT TGAAAAACAT GTTCCTGCCA CAAAAGAAAA TGATAAAAGA GCAAATAGAA 
TGGCTAATAG AGCACAAATA CATCAGAAGA GATGAATCTG ATATCAACAC TTTCATATAT 
ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL5 ( XM_025266946.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122731.1 Bubalus bubalis cullin 5 (CUL5), transcript variant X3, mRNA.

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CloneID	OBu50898
Clone ID Related Accession (Same CDS sequence)	XM_025266947.1 , XM_025266946.1
Accession Version	XM_025266946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	cullin-5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAC AGGGCAGCAA TAAAAAATCA AATGTAGAAG ACAGTATTGT TAGAAAGCTC 
ATGCTAGATA CATGGAATGA ATCAATCTTT TCAAATATAA AAAACAGACT TCAAGATAGT 
GCAATGAAGC TGGTCCATGC TGAAAGATTA GGAGAAGCTT TTGATTCTCA GCTTGTCATT 
GGAGTAAGAG AATCCTATGT TAACCTTTGT TCTAATCCAG AGGATAAGCT CCAAATTTAT 
AGGGACAATT TTGAGAAGGC ATACTTGGAT TCAACAGAGA GATTTTATAG AACACAGGCA 
CCCTCGTATT TACAACAAAA TGGTGTACAG AATTATATGA AATATGCAGA TGCTAAATTA 
AAAGAAGAAG AAAAACGAGC CCTACGTTAT TTAGAAACAA GACGAGAATG TAACTCTGTT 
GAAGCACTCA TGGAATGCTG TGTAAATGCC CTGGTGACAT CCTTTAAAGA GACTATCTTA 
GCAGAGTGCC AAGGCATGAT CAAGCGAAAT GAAACTGAAA AATTACATTT AATGTTTTCA 
TTGATGGACA AAGTTCCTAA TGGAATAGAA CCAATGTTAA AAGACTTGGA GGAGCATATC 
ATCAGTGCGG GCCTGGCAGA TATGGTAGCA GCTGCTGAAA CTATTACTAC TGACTCTGAG 
AAGTATGTTG AGCAGTTACT TACACTATTT AATAGATTCA GTAAACTTGT CAAAGAAGCT 
TTTCAAGATG ACCCACGATT TCTTACTGCA AGAGACAAGG CATATAAAGC AGTTGTTAAT 
GATGCTACCA TATTTAAACT TGAATTACCT TTGAAGCAGA AGGGGGTGGG ATTAAAAACT 
CAGCCTGAAT CAAAATGCCC TGAGCTGCTT GCCAATTACT GTGACATGTT GCTAAGAAAA 
ACGCCATTAA GCAAAAAGCT AACCTCTGAA GAGATTGAAG CAAAGCTTAA AGAAGTGCTC 
TTGGTACTTA AATACGTACA AAACAAAGAC GTTTTTATGA GATATCACAA AGCTCATTTG 
ACACGACGTC TTATATTAGA CATCTCTGCT GATAGTGAAA TTGAAGAGAA TATGGTAGAG 
TGGCTAAGAG AAGTTGGTAT GCCAGCAGAT TATGTAAACA AGCTTGCTAG AATGTTTCAG 
GACATAAAAG TATCTGAAGA TTTGAACCAA GCTTTTAAGG AAATGCACAA AAACAATAAA 
TTGGCGTTAC CAGCTGATTC AGTTAATATA AAAATTCTGA ATGCTGGTGC CTGGTCAAGA 
AGCTCTGAAA AAGTCTTTGT CTCACTTCCT ACTGAACTGG AGGATTTGAT ACCTGAAGTA 
GAAGAATTCT ACAAAAAGAA CCATAGTGGA AGAAAATTAC ATTGGCATCA TCTCATGTCA 
AATGGAATTA TAACATTTAA GAATGAAGTA GGCCAATATG ATTTGGAGGT AACAACGTTT 
CAGCTGGCTG TATTGTTTGC ATGGAACCAA AGACCCAGAG AGAAAATCAG CTTTGAAAAT 
CTAAAACTTG CAACTGAACT CCCTGATGCT GAGCTTAGGA GGACTTTATG GTCTTTAGTA 
GCTTTTCCAA AGCTCAAACG GCAAGTTTTA TTGTATGAAC CTCAAGTCAA CTCACCCAAA 
GACTTTACAG AAGGTACCCT CTTCTCAGTG AACCAGGAGT TCAGTTTAAT AAAAAATGCA 
AAAGTTCAGA AAAGGGGTAA AATCAACTTG ATTGGACGCT TGCAGCTCAC TACAGAAAGA 
ATGAGAGAGG AAGAGAATGA AGGAATAGTT CAACTAAGAA TACTGAGAAC CCAGGAAGCT 
ATCATACAAA TAATGAAGAT GAGAAAGAAA ATTAGTAATG CTCAGCTGCA GACTGAATTA 
GTAGAAATTT TGAAAAACAT GTTCCTGCCA CAAAAGAAAA TGATAAAAGA GCAAATAGAA 
TGGCTAATAG AGCACAAATA CATCAGAAGA GATGAATCTG ATATCAACAC TTTCATATAT 
ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122731.1
CDS	680..2668
Translation	MGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

Target ORF information:

RefSeq Version	XM_025266946.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cullin 5 (CUL5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266946.1

ORF Insert Sequence:

ATGGGTAAAC AGGGCAGCAA TAAAAAATCA AATGTAGAAG ACAGTATTGT TAGAAAGCTC 
ATGCTAGATA CATGGAATGA ATCAATCTTT TCAAATATAA AAAACAGACT TCAAGATAGT 
GCAATGAAGC TGGTCCATGC TGAAAGATTA GGAGAAGCTT TTGATTCTCA GCTTGTCATT 
GGAGTAAGAG AATCCTATGT TAACCTTTGT TCTAATCCAG AGGATAAGCT CCAAATTTAT 
AGGGACAATT TTGAGAAGGC ATACTTGGAT TCAACAGAGA GATTTTATAG AACACAGGCA 
CCCTCGTATT TACAACAAAA TGGTGTACAG AATTATATGA AATATGCAGA TGCTAAATTA 
AAAGAAGAAG AAAAACGAGC CCTACGTTAT TTAGAAACAA GACGAGAATG TAACTCTGTT 
GAAGCACTCA TGGAATGCTG TGTAAATGCC CTGGTGACAT CCTTTAAAGA GACTATCTTA 
GCAGAGTGCC AAGGCATGAT CAAGCGAAAT GAAACTGAAA AATTACATTT AATGTTTTCA 
TTGATGGACA AAGTTCCTAA TGGAATAGAA CCAATGTTAA AAGACTTGGA GGAGCATATC 
ATCAGTGCGG GCCTGGCAGA TATGGTAGCA GCTGCTGAAA CTATTACTAC TGACTCTGAG 
AAGTATGTTG AGCAGTTACT TACACTATTT AATAGATTCA GTAAACTTGT CAAAGAAGCT 
TTTCAAGATG ACCCACGATT TCTTACTGCA AGAGACAAGG CATATAAAGC AGTTGTTAAT 
GATGCTACCA TATTTAAACT TGAATTACCT TTGAAGCAGA AGGGGGTGGG ATTAAAAACT 
CAGCCTGAAT CAAAATGCCC TGAGCTGCTT GCCAATTACT GTGACATGTT GCTAAGAAAA 
ACGCCATTAA GCAAAAAGCT AACCTCTGAA GAGATTGAAG CAAAGCTTAA AGAAGTGCTC 
TTGGTACTTA AATACGTACA AAACAAAGAC GTTTTTATGA GATATCACAA AGCTCATTTG 
ACACGACGTC TTATATTAGA CATCTCTGCT GATAGTGAAA TTGAAGAGAA TATGGTAGAG 
TGGCTAAGAG AAGTTGGTAT GCCAGCAGAT TATGTAAACA AGCTTGCTAG AATGTTTCAG 
GACATAAAAG TATCTGAAGA TTTGAACCAA GCTTTTAAGG AAATGCACAA AAACAATAAA 
TTGGCGTTAC CAGCTGATTC AGTTAATATA AAAATTCTGA ATGCTGGTGC CTGGTCAAGA 
AGCTCTGAAA AAGTCTTTGT CTCACTTCCT ACTGAACTGG AGGATTTGAT ACCTGAAGTA 
GAAGAATTCT ACAAAAAGAA CCATAGTGGA AGAAAATTAC ATTGGCATCA TCTCATGTCA 
AATGGAATTA TAACATTTAA GAATGAAGTA GGCCAATATG ATTTGGAGGT AACAACGTTT 
CAGCTGGCTG TATTGTTTGC ATGGAACCAA AGACCCAGAG AGAAAATCAG CTTTGAAAAT 
CTAAAACTTG CAACTGAACT CCCTGATGCT GAGCTTAGGA GGACTTTATG GTCTTTAGTA 
GCTTTTCCAA AGCTCAAACG GCAAGTTTTA TTGTATGAAC CTCAAGTCAA CTCACCCAAA 
GACTTTACAG AAGGTACCCT CTTCTCAGTG AACCAGGAGT TCAGTTTAAT AAAAAATGCA 
AAAGTTCAGA AAAGGGGTAA AATCAACTTG ATTGGACGCT TGCAGCTCAC TACAGAAAGA 
ATGAGAGAGG AAGAGAATGA AGGAATAGTT CAACTAAGAA TACTGAGAAC CCAGGAAGCT 
ATCATACAAA TAATGAAGAT GAGAAAGAAA ATTAGTAATG CTCAGCTGCA GACTGAATTA 
GTAGAAATTT TGAAAAACAT GTTCCTGCCA CAAAAGAAAA TGATAAAAGA GCAAATAGAA 
TGGCTAATAG AGCACAAATA CATCAGAAGA GATGAATCTG ATATCAACAC TTTCATATAT 
ATGGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CUL5 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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