N4BP2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following N4BP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N4BP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu23249 XM_025290139.1
Latest version!
Bubalus bubalis NEDD4-binding protein 2 (LOC102396318), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBu23249 XM_006068243.1
Latest version!
Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBu23249 XM_006068242.1
Latest version!
Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBu23250 XM_006068244.2
Latest version!
Bubalus bubalis NEDD4-binding protein 2 (LOC102396318), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OBu23250 XM_006068244.1 Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu23249
    Clone ID Related Accession (Same CDS sequence) XM_025290139.1 , XM_006068242.1 , XM_006068243.1
    Accession Version XM_025290139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025145924.1
    CDS36..5339
    Translation

    Target ORF information:

    RefSeq Version XM_025290139.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NEDD4-binding protein 2 (LOC102396318), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025290139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu23249
    Clone ID Related Accession (Same CDS sequence) XM_025290139.1 , XM_006068242.1 , XM_006068243.1
    Accession Version XM_006068242.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785306.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068304.1
    CDS230..5533
    Translation

    Target ORF information:

    RefSeq Version XM_006068242.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu23249
    Clone ID Related Accession (Same CDS sequence) XM_025290139.1 , XM_006068242.1 , XM_006068243.1
    Accession Version XM_006068243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product NEDD4-binding protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785306.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068305.1
    CDS230..5533
    Translation

    Target ORF information:

    RefSeq Version XM_006068243.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAGGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACCAC ACACTCTAAG 
    GAAGTTGTAT CCAGTGTTAC TAGTCATGAG GAGCCAACCA CTACTCTTCC TTCCATGTAT
    GAGACAAAGG TTGATCAGGA AGAACTCTTC ACCAGTGTCT CAGAGATGTT TTCTGATCTG
    GATCCTGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCCATG
    GATTGTCTAT TAGAATTATC AGCCACTGAT GCTAAGATAG AAGAATCGTC TTCAACAAAC
    TTCGTTGCTT CTGAGAAACA AGTAAATGCA GTGAAATGTG AAATAATGGA AAAATGTCCT
    CCAGAAGAAG AGAGTGAAGA TTTAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTAGATA CTTTAATACA AAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATAACCAA
    GCATACTCAT TTTTGCCTTT GCAAGATGTT AATAATTTTA GTGACCCGAG TTCATTTGTA
    AGTTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACCC AAAATATGGA TTTGAACAAT
    GAAAATTTAG AGCATTCTGT CTCTGCATTA AGTTCAAATT CCTTAACTTC ACATTCAGTT
    TCAAATGGGT CCAAAAGTTT TACAAAAGAT AACACAGTGG CATTGGAAGA TTCTCTTCTC
    AGTAGTTCTT TAAATGCAGA TAATGACACT TTGATGGATG GCAGCAACCA AATTCAGAAA
    GAGCTTTCGG AATCTGAGTG TGTTAAGACT CAGTTCTCTC AAGCCCCTGT AGATTTGGAT
    GCTAATGAGC TTCAGGCTCC TCTAAACCTT GCTGTCCAAA ATCCAGGGAT TGGTTTAATA
    GGTACAGGTG GAGATCAGAA GTCCACGTCT GTCCCTGATG TTTTTGTACC TTCTGAAGAA
    TTCAGTTTCC AGCCACACAA ACATACTGAA CTGCCACCAA AGGGGAAGGA TGTGAATTAC
    TGCCCAGTAC TAACCCCTCT CCCTCTACTG CTGCCTCCTC CCCCCCCTCC ACCAATGTGG
    AATCCAATGA TTCCTGCTTT TGACCTCTTT CAAGCAAACC ATGGCTTTGT AGCTCCTGTT
    GTAACCACAG CTGCACACTG GAGGCCTGTC AATTATACGT TTCCACCCCC AGTTATTTCT
    CATACTTCCC CAACAAAGGT ATGGAGAAGT AAAGAGGGAA CAAGTGCTTA CCAGGTACAA
    GAAACTCCAG TTTCTCAGGT TGTAAGAAAG AAGACATCAT CTTATGTTGG ACTAGTTCTC
    GTTCTTCTCA GAGGCCTTCC AGGATCAGGA AAATCTTCTT TGGCAAGGAC TTTGCAAGAG
    GATAATCCAA GTGGAGTTAT TCTTAGTACT GATGATTATT TTTACATAAA TGGACAATAC
    CAGTTTGATG TAAGATACTT AGGAGAAGCA CATGAATGGA ACCAGAATCG TGCAAAAGAA
    GCATTTGAGA AGAAAGTGTC TCCTGTAATA ATAGATAATA CAAACCTACA GGCATGGGAA
    ATGAAGCCAT ATGTTGCTTT GTCTCAAAAA CATAAATATA AAGTCCTTTT CCGGGAACCG
    GATACATGGT GGAAATTCAA ACCGAAGGAA CTTGCAAGGC GTAATATTCA TGGGATAAGC
    AAAGAAAAAA TAACAAGAAT GTTGGAACAC TATCAACGTT TTGTTTCAGT GCCAATAATC
    ATGAGTTCTT CAGTTCCAGA GAAAATGGAA CGCGTTGAAT TGTGTGCATA TTCTTGTGAC
    AGAAGTACTA GCCCAAGAGA CAATGAAGAT ATTACTTCTG AAAAAGAAGA AAATGTTTTA
    TCCTCGTCTT TGAAGCATCT AGAGTTAACT GAAGAGAAGA AACTTGATGT GACCAAAGAA
    AATATGTTAC CTGAGAATAT TGCATATCTC CCTAATGCAG ATTTAAACAA AGGAAAAAAA
    GAAATAAGTG ATATGAATCC CAATATTCAG AATGCTTTCA TTCAGGAAGC TCCAAACACG
    TACTTTCCTG ATTCTGAAAG TGAAGTACAA GTTACAGACA AAAGTGAAAA AGAAGAGCCA
    GTAGAAATGG CTCTTGAAAA AGAGTGTAGT GAAACCAATG CAGATAGTTT GTTAGAGAGA
    ATGTCACCAA GTAGTTGTTA TGGTGGAAAT AATCAAGGGG ACCGTGGTCT TTCAACTCCT
    GTACCCAATC AAAATGAAAA ATCTTTATCT AGTGAAATAT TGGAAGAAAG ACCGGCAGTA
    AAGAAAAAAG CCTTTGGAAA ACAAAAGGGC AAATCAACTT TGGAAAAGTT TCCAAAACAC
    GAGCTGTCTT TTGTTGGTGA CTGGCCAATT GATAAGACTA TTGGTCAGAG GACAAAAAGG
    AACCGAAAAA TGGAAAAAGC CTCATCTGTA CAAAATGACA AAAAGTATAA TCGCCCTCAG
    TCACATAAAA TATTAGATAA TAGTTTATCT GTGAGTGTAG ATTGTATGCA GCCACAAGGA
    CCTCTATATG AAAATGTAGA GGATGACGAC AGGTCACAGT GTGGTGATGC CTCGGAACCT
    TTCAGTAGCT GTGAATATGA TACTTACAAA AACCCGGAAA GAGATTCATT CAACATTGTG
    GGTGACTGGC CTTCCCTCGA TTCGTTAGCT CAGAGAGAGC ACAGATCAAG AATACCAAAG
    GCTGGCTTAA ATGAACCCAG CCTAGAATTT GGAACCAACA ACAGTATGAA TGAAATATCC
    TTATACTCTT CACATGAGGC CTGTTGGGGC ACAAGCCCTG AAGAGCTAAA AACATTGGGT
    GGTTGTACTC GAGGAAGTTC TGAAATGTTG CCCAATGAAA TGACCTATGA GAGTAAGTCT
    TGTCCAAGTA AAAAGATTCA TAGGGAACAC ACATTGTCTC TTCCTTATAT CAATAATGTG
    CCAACTGTCC CTGGAGAAAT AGGACCACAA ACTTTAGCTG AATTTTCAGA AGAAAAGACT
    AAAGGAGTCC CTGGAATAGG AGTAGGCATG TGTACCCAGA CTGAACCACA GGATTTTGCT
    CTCTTATGGA AAGTAGAAAA AAATAAGATT AGTGTTTCTG ATTCTCTGAG AGTATTAACA
    GGAAGATTAG ATGGATTTAA ACCCAAAACT TTCAATATTA ACACAAAATT GGATGTTCAA
    GAAGCAATTC CATATAGGGT AATGTATGAC AAAAGCACAT ATGTGGAAGA AAGTGAGCTC
    ACCAGTGCTG ATGAATCTGA AAATCTTAAC ATTCTTTGTA AACTATTTGG ATCTTTTTCA
    TTAGAAGCCC TAAAAGACTT ATATGAGAGG TGTAACAAAG ATATTATTTG GGCCACAAGC
    CTTTTATTGG ATTCTGAAAC TAAATTATGT GAGGATACTG AATTTGAGAA TATAAAAAAA
    TCATACGATG AAGCACAAAT TGGGCCATTT TCTCTGGGAC TGAATTTGAA GGAAATTATT
    AGCCAAAGAG GAAGCTTAGA GGATTCTAAT TTTTCTGTGC CAGAATTTAG CAATGGAATT
    GATATCAATA ACACTAATGT ACAGTCTACC TGTAATTCCA AAAAGGAAAA CTTAGAGCAG
    GCAGAAATAA GAGCTATACC TACTGAAAAA CCTAGATTAA TAGCAAGTAT GCTTCCTACT
    GTAGAGCTAA ATAATAGTAA TGAAGTACTT CCTAATAGTC AAACAGAACT TTCAGGTTTA
    TATAATGTGA AGCAGTTTTT TCCAGATACT CTAAAAGCTA CTACCACTAA AACTGTGAAT
    GAAATGGAAA AGAATTCAGA AGTTGCAGAG ACTGGAGGTA ACATACCACC TTCTTTGAAT
    TTGTCTGACA TTTTGAACTC TGTATCAAGC ACTTCAAATC TTGAGTTACA TGAAGAAACT
    TATTTTACTA ACTCTTTTGA AATAAATAAA AATGAAAATC TTCCAAAGGA CTATGTGAAA
    TTTCCAAACA CAGAAGATTT TATGATTGAA GAGGAACAGG AAATGGAGAC AATTCTAATG
    GCAGGGAGTG CTTTGTCAGC TGGAGTTACT GGAGAAGATA AAGCTGAGAC ACTGAATCCC
    ACTCCAGTGA CAACCAAATC TCTGACCATA GACTGTCTTG AATTGGCATT GCCCCCTGAA
    CTGGCTTTTC AGCTCAATGA ATTATTTGGC CCTGTTGGTA TTGATTCAGG GTCCCTAACA
    GTTGAGGATT GTGTGGTTCA TATAGATCTG AATCTGGCTA AAGTGATTCA TGAGAAATGG
    AAAGAATCTG TAATGGAGCG ACAAAGACAA GAAGAGGTAT CTTGTGGCAA GTTAATGCAA
    GATCCTTCCC TGGTTGGGCA TATTGGTCTT GATAATTCAG AACAAAAATC ATCTCAGAGA
    ACAGGCAAAA AATTACTGAA GACTTTAGCA GCACCTGAAA TGCTACCCTT ATTGGATCAT
    TGGAACACTC AGACTAAAAA AGTATCACTC AGAGAAATAA TGTCAGAAGA AATTGCCTTA
    CAGGAAAAGC ATGATTTGAA AAGGGAGACG CTTATGTTTG AGAAAGATTG TGCCACTAAG
    CTAAAGGAGA AGCAACTCTT TAAGATATTT CCAGCCATTA ACCAAAATTT CCTGGTGGAC
    ATTTTCAAGG ACCACAACTA TTCATTAGAA CACACAGTGC AATTTCTAAA TTGTGTTCTT
    GAAGGAGACC CTGTAAAAAC AGTGGTAGCA CAAGAATTTG TTCACCAAAA TGAGAATATC
    ACTTCTCATA CTGCACAGAA GTCTAAGGAG AAAAAGGCAA AGAAATTGAA AGAGACTGAA
    GATATACCAA GTGAACCATC TTTCCAGGAC TTTGAGTACC CAGAGTATGA TGACTACAGG
    GCAGAGGCTT TCCTGCACCA GCAGAAGAGG ATGGAATGCT ACAGCAAGGC AAAAGAAGCT
    TATCGGATGG GGAAGAAAAA CGTTGCCACA TTTTATGCCC AGCAGGGTAG TCTCCATGAG
    CAGAAGATGA AAGAAGCTAA TCACCTTGCT GCCGTGGAGA TATTTGAAAA AGTCAATGCC
    TCCCTACTGC CGCAGAATGT TCTAGACCTC CATGGGCTGC ATGTGGATGA GGCTATAGAA
    CATTTGACAA CAGTTTTACA GCAGAAAACT GAAGAATTTA AACAGAATGG TGGTAAGCCC
    TATCTCTCTG TGATAACGGG GAGAGGAAAC CACAGCCAGG GAGGGGTTGC GCGCATCAAA
    CCAGCCGTCA TTAAATACCT CACAAGCCAT AACTTTAGAT TCGCTGAAAT TAAACCAGGG
    TGCTTGAAAG TCATGCTAAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu23250
    Clone ID Related Accession (Same CDS sequence) XM_006068244.2 , XM_006068244.1
    Accession Version XM_006068244.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product NEDD4-binding protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037551.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006068244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCAGCCACT GATGCTAAGA TAGAAGAATC GTCTTCAACA 
    AACTTCGTTG CTTCTGAGAA ACAAGTAAAT GCAGTGAAAT GTGAAATAAT GGAAAAATGT
    CCTCCAGAAG AAGAGAGTGA AGATTTAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTAG ATACTTTAAT ACAAAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATAAC
    CAAGCATACT CATTTTTGCC TTTGCAAGAT GTTAATAATT TTAGTGACCC GAGTTCATTT
    GTAAGTTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CCCAAAATAT GGATTTGAAC
    AATGAAAATT TAGAGCATTC TGTCTCTGCA TTAAGTTCAA ATTCCTTAAC TTCACATTCA
    GTTTCAAATG GGTCCAAAAG TTTTACAAAA GATAACACAG TGGCATTGGA AGATTCTCTT
    CTCAGTAGTT CTTTAAATGC AGATAATGAC ACTTTGATGG ATGGCAGCAA CCAAATTCAG
    AAAGAGCTTT CGGAATCTGA GTGTGTTAAG ACTCAGTTCT CTCAAGCCCC TGTAGATTTG
    GATGCTAATG AGCTTCAGGC TCCTCTAAAC CTTGCTGTCC AAAATCCAGG GATTGGTTTA
    ATAGGTACAG GTGGAGATCA GAAGTCCACG TCTGTCCCTG ATGTTTTTGT ACCTTCTGAA
    GAATTCAGTT TCCAGCCACA CAAACATACT GAACTGCCAC CAAAGGGGAA GGATGTGAAT
    TACTGCCCAG TACTAACCCC TCTCCCTCTA CTGCTGCCTC CTCCCCCCCC TCCACCAATG
    TGGAATCCAA TGATTCCTGC TTTTGACCTC TTTCAAGCAA ACCATGGCTT TGTAGCTCCT
    GTTGTAACCA CAGCTGCACA CTGGAGGCCT GTCAATTATA CGTTTCCACC CCCAGTTATT
    TCTCATACTT CCCCAACAAA GGTATGGAGA AGTAAAGAGG GAACAAGTGC TTACCAGGTA
    CAAGAAACTC CAGTTTCTCA GGTTGTAAGA AAGAAGACAT CATCTTATGT TGGACTAGTT
    CTCGTTCTTC TCAGAGGCCT TCCAGGATCA GGAAAATCTT CTTTGGCAAG GACTTTGCAA
    GAGGATAATC CAAGTGGAGT TATTCTTAGT ACTGATGATT ATTTTTACAT AAATGGACAA
    TACCAGTTTG ATGTAAGATA CTTAGGAGAA GCACATGAAT GGAACCAGAA TCGTGCAAAA
    GAAGCATTTG AGAAGAAAGT GTCTCCTGTA ATAATAGATA ATACAAACCT ACAGGCATGG
    GAAATGAAGC CATATGTTGC TTTGTCTCAA AAACATAAAT ATAAAGTCCT TTTCCGGGAA
    CCGGATACAT GGTGGAAATT CAAACCGAAG GAACTTGCAA GGCGTAATAT TCATGGGATA
    AGCAAAGAAA AAATAACAAG AATGTTGGAA CACTATCAAC GTTTTGTTTC AGTGCCAATA
    ATCATGAGTT CTTCAGTTCC AGAGAAAATG GAACGCGTTG AATTGTGTGC ATATTCTTGT
    GACAGAAGTA CTAGCCCAAG AGACAATGAA GATATTACTT CTGAAAAAGA AGAAAATGTT
    TTATCCTCGT CTTTGAAGCA TCTAGAGTTA ACTGAAGAGA AGAAACTTGA TGTGACCAAA
    GAAAATATGT TACCTGAGAA TATTGCATAT CTCCCTAATG CAGATTTAAA CAAAGGAAAA
    AAAGAAATAA GTGATATGAA TCCCAATATT CAGAATGCTT TCATTCAGGA AGCTCCAAAC
    ACGTACTTTC CTGATTCTGA AAGTGAAGTA CAAGTTACAG ACAAAAGTGA AAAAGAAGAG
    CCAGTAGAAA TGGCTCTTGA AAAAGAGTGT AGTGAAACCA ATGCAGATAG TTTGTTAGAG
    AGAATGTCAC CAAGTAGTTG TTATGGTGGA AATAATCAAG GGGACCGTGG TCTTTCAACT
    CCTGTACCCA ATCAAAATGA AAAATCTTTA TCTAGTGAAA TATTGGAAGA AAGACCGGCA
    GTAAAGAAAA AAGCCTTTGG AAAACAAAAG GGCAAATCAA CTTTGGAAAA GTTTCCAAAA
    CACGAGCTGT CTTTTGTTGG TGACTGGCCA ATTGATAAGA CTATTGGTCA GAGGACAAAA
    AGGAACCGAA AAATGGAAAA AGCCTCATCT GTACAAAATG ACAAAAAGTA TAATCGCCCT
    CAGTCACATA AAATATTAGA TAATAGTTTA TCTGTGAGTG TAGATTGTAT GCAGCCACAA
    GGACCTCTAT ATGAAAATGT AGAGGATGAC GACAGGTCAC AGTGTGGTGA TGCCTCGGAA
    CCTTTCAGTA GCTGTGAATA TGATACTTAC AAAAACCCGG AAAGAGATTC ATTCAACATT
    GTGGGTGACT GGCCTTCCCT CGATTCGTTA GCTCAGAGAG AGCACAGATC AAGAATACCA
    AAGGCTGGCT TAAATGAACC CAGCCTAGAA TTTGGAACCA ACAACAGTAT GAATGAAATA
    TCCTTATACT CTTCACATGA GGCCTGTTGG GGCACAAGCC CTGAAGAGCT AAAAACATTG
    GGTGGTTGTA CTCGAGGAAG TTCTGAAATG TTGCCCAATG AAATGACCTA TGAGAGTAAG
    TCTTGTCCAA GTAAAAAGAT TCATAGGGAA CACACATTGT CTCTTCCTTA TATCAATAAT
    GTGCCAACTG TCCCTGGAGA AATAGGACCA CAAACTTTAG CTGAATTTTC AGAAGAAAAG
    ACTAAAGGAG TCCCTGGAAT AGGAGTAGGC ATGTGTACCC AGACTGAACC ACAGGATTTT
    GCTCTCTTAT GGAAAGTAGA AAAAAATAAG ATTAGTGTTT CTGATTCTCT GAGAGTATTA
    ACAGGAAGAT TAGATGGATT TAAACCCAAA ACTTTCAATA TTAACACAAA ATTGGATGTT
    CAAGAAGCAA TTCCATATAG GGTAATGTAT GACAAAAGCA CATATGTGGA AGAAAGTGAG
    CTCACCAGTG CTGATGAATC TGAAAATCTT AACATTCTTT GTAAACTATT TGGATCTTTT
    TCATTAGAAG CCCTAAAAGA CTTATATGAG AGGTGTAACA AAGATATTAT TTGGGCCACA
    AGCCTTTTAT TGGATTCTGA AACTAAATTA TGTGAGGATA CTGAATTTGA GAATATAAAA
    AAATCATACG ATGAAGCACA AATTGGGCCA TTTTCTCTGG GACTGAATTT GAAGGAAATT
    ATTAGCCAAA GAGGAAGCTT AGAGGATTCT AATTTTTCTG TGCCAGAATT TAGCAATGGA
    ATTGATATCA ATAACACTAA TGTACAGTCT ACCTGTAATT CCAAAAAGGA AAACTTAGAG
    CAGGCAGAAA TAAGAGCTAT ACCTACTGAA AAACCTAGAT TAATAGCAAG TATGCTTCCT
    ACTGTAGAGC TAAATAATAG TAATGAAGTA CTTCCTAATA GTCAAACAGA ACTTTCAGGT
    TTATATAATG TGAAGCAGTT TTTTCCAGAT ACTCTAAAAG CTACTACCAC TAAAACTGTG
    AATGAAATGG AAAAGAATTC AGAAGTTGCA GAGACTGGAG GTAACATACC ACCTTCTTTG
    AATTTGTCTG ACATTTTGAA CTCTGTATCA AGCACTTCAA ATCTTGAGTT ACATGAAGAA
    ACTTATTTTA CTAACTCTTT TGAAATAAAT AAAAATGAAA ATCTTCCAAA GGACTATGTG
    AAATTTCCAA ACACAGAAGA TTTTATGATT GAAGAGGAAC AGGAAATGGA GACAATTCTA
    ATGGCAGGGA GTGCTTTGTC AGCTGGAGTT ACTGGAGAAG ATAAAGCTGA GACACTGAAT
    CCCACTCCAG TGACAACCAA ATCTCTGACC ATAGACTGTC TTGAATTGGC ATTGCCCCCT
    GAACTGGCTT TTCAGCTCAA TGAATTATTT GGCCCTGTTG GTATTGATTC AGGGTCCCTA
    ACAGTTGAGG ATTGTGTGGT TCATATAGAT CTGAATCTGG CTAAAGTGAT TCATGAGAAA
    TGGAAAGAAT CTGTAATGGA GCGACAAAGA CAAGAAGAGG TATCTTGTGG CAAGTTAATG
    CAAGATCCTT CCCTGGTTGG GCATATTGGT CTTGATAATT CAGAACAAAA ATCATCTCAG
    AGAACAGGCA AAAAATTACT GAAGACTTTA GCAGCACCTG AAATGCTACC CTTATTGGAT
    CATTGGAACA CTCAGACTAA AAAAGTATCA CTCAGAGAAA TAATGTCAGA AGAAATTGCC
    TTACAGGAAA AGCATGATTT GAAAAGGGAG ACGCTTATGT TTGAGAAAGA TTGTGCCACT
    AAGCTAAAGG AGAAGCAACT CTTTAAGATA TTTCCAGCCA TTAACCAAAA TTTCCTGGTG
    GACATTTTCA AGGACCACAA CTATTCATTA GAACACACAG TGCAATTTCT AAATTGTGTT
    CTTGAAGGAG ACCCTGTAAA AACAGTGGTA GCACAAGAAT TTGTTCACCA AAATGAGAAT
    ATCACTTCTC ATACTGCACA GAAGTCTAAG GAGAAAAAGG CAAAGAAATT GAAAGAGACT
    GAAGATATAC CAAGTGAACC ATCTTTCCAG GACTTTGAGT ACCCAGAGTA TGATGACTAC
    AGGGCAGAGG CTTTCCTGCA CCAGCAGAAG AGGATGGAAT GCTACAGCAA GGCAAAAGAA
    GCTTATCGGA TGGGGAAGAA AAACGTTGCC ACATTTTATG CCCAGCAGGG TAGTCTCCAT
    GAGCAGAAGA TGAAAGAAGC TAATCACCTT GCTGCCGTGG AGATATTTGA AAAAGTCAAT
    GCCTCCCTAC TGCCGCAGAA TGTTCTAGAC CTCCATGGGC TGCATGTGGA TGAGGCTATA
    GAACATTTGA CAACAGTTTT ACAGCAGAAA ACTGAAGAAT TTAAACAGAA TGGTGGTAAG
    CCCTATCTCT CTGTGATAAC GGGGAGAGGA AACCACAGCC AGGGAGGGGT TGCGCGCATC
    AAACCAGCCG TCATTAAATA CCTCACAAGC CATAACTTTA GATTCGCTGA AATTAAACCA
    GGGTGCTTGA AAGTCATGCT AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068306.1
    CDS239..5305
    Translation

    Target ORF information:

    RefSeq Version XM_006068244.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NEDD4-binding protein 2 (LOC102396318), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCAGCCACT GATGCTAAGA TAGAAGAATC GTCTTCAACA 
    AACTTCGTTG CTTCTGAGAA ACAAGTAAAT GCAGTGAAAT GTGAAATAAT GGAAAAATGT
    CCTCCAGAAG AAGAGAGTGA AGATTTAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTAG ATACTTTAAT ACAAAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATAAC
    CAAGCATACT CATTTTTGCC TTTGCAAGAT GTTAATAATT TTAGTGACCC GAGTTCATTT
    GTAAGTTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CCCAAAATAT GGATTTGAAC
    AATGAAAATT TAGAGCATTC TGTCTCTGCA TTAAGTTCAA ATTCCTTAAC TTCACATTCA
    GTTTCAAATG GGTCCAAAAG TTTTACAAAA GATAACACAG TGGCATTGGA AGATTCTCTT
    CTCAGTAGTT CTTTAAATGC AGATAATGAC ACTTTGATGG ATGGCAGCAA CCAAATTCAG
    AAAGAGCTTT CGGAATCTGA GTGTGTTAAG ACTCAGTTCT CTCAAGCCCC TGTAGATTTG
    GATGCTAATG AGCTTCAGGC TCCTCTAAAC CTTGCTGTCC AAAATCCAGG GATTGGTTTA
    ATAGGTACAG GTGGAGATCA GAAGTCCACG TCTGTCCCTG ATGTTTTTGT ACCTTCTGAA
    GAATTCAGTT TCCAGCCACA CAAACATACT GAACTGCCAC CAAAGGGGAA GGATGTGAAT
    TACTGCCCAG TACTAACCCC TCTCCCTCTA CTGCTGCCTC CTCCCCCCCC TCCACCAATG
    TGGAATCCAA TGATTCCTGC TTTTGACCTC TTTCAAGCAA ACCATGGCTT TGTAGCTCCT
    GTTGTAACCA CAGCTGCACA CTGGAGGCCT GTCAATTATA CGTTTCCACC CCCAGTTATT
    TCTCATACTT CCCCAACAAA GGTATGGAGA AGTAAAGAGG GAACAAGTGC TTACCAGGTA
    CAAGAAACTC CAGTTTCTCA GGTTGTAAGA AAGAAGACAT CATCTTATGT TGGACTAGTT
    CTCGTTCTTC TCAGAGGCCT TCCAGGATCA GGAAAATCTT CTTTGGCAAG GACTTTGCAA
    GAGGATAATC CAAGTGGAGT TATTCTTAGT ACTGATGATT ATTTTTACAT AAATGGACAA
    TACCAGTTTG ATGTAAGATA CTTAGGAGAA GCACATGAAT GGAACCAGAA TCGTGCAAAA
    GAAGCATTTG AGAAGAAAGT GTCTCCTGTA ATAATAGATA ATACAAACCT ACAGGCATGG
    GAAATGAAGC CATATGTTGC TTTGTCTCAA AAACATAAAT ATAAAGTCCT TTTCCGGGAA
    CCGGATACAT GGTGGAAATT CAAACCGAAG GAACTTGCAA GGCGTAATAT TCATGGGATA
    AGCAAAGAAA AAATAACAAG AATGTTGGAA CACTATCAAC GTTTTGTTTC AGTGCCAATA
    ATCATGAGTT CTTCAGTTCC AGAGAAAATG GAACGCGTTG AATTGTGTGC ATATTCTTGT
    GACAGAAGTA CTAGCCCAAG AGACAATGAA GATATTACTT CTGAAAAAGA AGAAAATGTT
    TTATCCTCGT CTTTGAAGCA TCTAGAGTTA ACTGAAGAGA AGAAACTTGA TGTGACCAAA
    GAAAATATGT TACCTGAGAA TATTGCATAT CTCCCTAATG CAGATTTAAA CAAAGGAAAA
    AAAGAAATAA GTGATATGAA TCCCAATATT CAGAATGCTT TCATTCAGGA AGCTCCAAAC
    ACGTACTTTC CTGATTCTGA AAGTGAAGTA CAAGTTACAG ACAAAAGTGA AAAAGAAGAG
    CCAGTAGAAA TGGCTCTTGA AAAAGAGTGT AGTGAAACCA ATGCAGATAG TTTGTTAGAG
    AGAATGTCAC CAAGTAGTTG TTATGGTGGA AATAATCAAG GGGACCGTGG TCTTTCAACT
    CCTGTACCCA ATCAAAATGA AAAATCTTTA TCTAGTGAAA TATTGGAAGA AAGACCGGCA
    GTAAAGAAAA AAGCCTTTGG AAAACAAAAG GGCAAATCAA CTTTGGAAAA GTTTCCAAAA
    CACGAGCTGT CTTTTGTTGG TGACTGGCCA ATTGATAAGA CTATTGGTCA GAGGACAAAA
    AGGAACCGAA AAATGGAAAA AGCCTCATCT GTACAAAATG ACAAAAAGTA TAATCGCCCT
    CAGTCACATA AAATATTAGA TAATAGTTTA TCTGTGAGTG TAGATTGTAT GCAGCCACAA
    GGACCTCTAT ATGAAAATGT AGAGGATGAC GACAGGTCAC AGTGTGGTGA TGCCTCGGAA
    CCTTTCAGTA GCTGTGAATA TGATACTTAC AAAAACCCGG AAAGAGATTC ATTCAACATT
    GTGGGTGACT GGCCTTCCCT CGATTCGTTA GCTCAGAGAG AGCACAGATC AAGAATACCA
    AAGGCTGGCT TAAATGAACC CAGCCTAGAA TTTGGAACCA ACAACAGTAT GAATGAAATA
    TCCTTATACT CTTCACATGA GGCCTGTTGG GGCACAAGCC CTGAAGAGCT AAAAACATTG
    GGTGGTTGTA CTCGAGGAAG TTCTGAAATG TTGCCCAATG AAATGACCTA TGAGAGTAAG
    TCTTGTCCAA GTAAAAAGAT TCATAGGGAA CACACATTGT CTCTTCCTTA TATCAATAAT
    GTGCCAACTG TCCCTGGAGA AATAGGACCA CAAACTTTAG CTGAATTTTC AGAAGAAAAG
    ACTAAAGGAG TCCCTGGAAT AGGAGTAGGC ATGTGTACCC AGACTGAACC ACAGGATTTT
    GCTCTCTTAT GGAAAGTAGA AAAAAATAAG ATTAGTGTTT CTGATTCTCT GAGAGTATTA
    ACAGGAAGAT TAGATGGATT TAAACCCAAA ACTTTCAATA TTAACACAAA ATTGGATGTT
    CAAGAAGCAA TTCCATATAG GGTAATGTAT GACAAAAGCA CATATGTGGA AGAAAGTGAG
    CTCACCAGTG CTGATGAATC TGAAAATCTT AACATTCTTT GTAAACTATT TGGATCTTTT
    TCATTAGAAG CCCTAAAAGA CTTATATGAG AGGTGTAACA AAGATATTAT TTGGGCCACA
    AGCCTTTTAT TGGATTCTGA AACTAAATTA TGTGAGGATA CTGAATTTGA GAATATAAAA
    AAATCATACG ATGAAGCACA AATTGGGCCA TTTTCTCTGG GACTGAATTT GAAGGAAATT
    ATTAGCCAAA GAGGAAGCTT AGAGGATTCT AATTTTTCTG TGCCAGAATT TAGCAATGGA
    ATTGATATCA ATAACACTAA TGTACAGTCT ACCTGTAATT CCAAAAAGGA AAACTTAGAG
    CAGGCAGAAA TAAGAGCTAT ACCTACTGAA AAACCTAGAT TAATAGCAAG TATGCTTCCT
    ACTGTAGAGC TAAATAATAG TAATGAAGTA CTTCCTAATA GTCAAACAGA ACTTTCAGGT
    TTATATAATG TGAAGCAGTT TTTTCCAGAT ACTCTAAAAG CTACTACCAC TAAAACTGTG
    AATGAAATGG AAAAGAATTC AGAAGTTGCA GAGACTGGAG GTAACATACC ACCTTCTTTG
    AATTTGTCTG ACATTTTGAA CTCTGTATCA AGCACTTCAA ATCTTGAGTT ACATGAAGAA
    ACTTATTTTA CTAACTCTTT TGAAATAAAT AAAAATGAAA ATCTTCCAAA GGACTATGTG
    AAATTTCCAA ACACAGAAGA TTTTATGATT GAAGAGGAAC AGGAAATGGA GACAATTCTA
    ATGGCAGGGA GTGCTTTGTC AGCTGGAGTT ACTGGAGAAG ATAAAGCTGA GACACTGAAT
    CCCACTCCAG TGACAACCAA ATCTCTGACC ATAGACTGTC TTGAATTGGC ATTGCCCCCT
    GAACTGGCTT TTCAGCTCAA TGAATTATTT GGCCCTGTTG GTATTGATTC AGGGTCCCTA
    ACAGTTGAGG ATTGTGTGGT TCATATAGAT CTGAATCTGG CTAAAGTGAT TCATGAGAAA
    TGGAAAGAAT CTGTAATGGA GCGACAAAGA CAAGAAGAGG TATCTTGTGG CAAGTTAATG
    CAAGATCCTT CCCTGGTTGG GCATATTGGT CTTGATAATT CAGAACAAAA ATCATCTCAG
    AGAACAGGCA AAAAATTACT GAAGACTTTA GCAGCACCTG AAATGCTACC CTTATTGGAT
    CATTGGAACA CTCAGACTAA AAAAGTATCA CTCAGAGAAA TAATGTCAGA AGAAATTGCC
    TTACAGGAAA AGCATGATTT GAAAAGGGAG ACGCTTATGT TTGAGAAAGA TTGTGCCACT
    AAGCTAAAGG AGAAGCAACT CTTTAAGATA TTTCCAGCCA TTAACCAAAA TTTCCTGGTG
    GACATTTTCA AGGACCACAA CTATTCATTA GAACACACAG TGCAATTTCT AAATTGTGTT
    CTTGAAGGAG ACCCTGTAAA AACAGTGGTA GCACAAGAAT TTGTTCACCA AAATGAGAAT
    ATCACTTCTC ATACTGCACA GAAGTCTAAG GAGAAAAAGG CAAAGAAATT GAAAGAGACT
    GAAGATATAC CAAGTGAACC ATCTTTCCAG GACTTTGAGT ACCCAGAGTA TGATGACTAC
    AGGGCAGAGG CTTTCCTGCA CCAGCAGAAG AGGATGGAAT GCTACAGCAA GGCAAAAGAA
    GCTTATCGGA TGGGGAAGAA AAACGTTGCC ACATTTTATG CCCAGCAGGG TAGTCTCCAT
    GAGCAGAAGA TGAAAGAAGC TAATCACCTT GCTGCCGTGG AGATATTTGA AAAAGTCAAT
    GCCTCCCTAC TGCCGCAGAA TGTTCTAGAC CTCCATGGGC TGCATGTGGA TGAGGCTATA
    GAACATTTGA CAACAGTTTT ACAGCAGAAA ACTGAAGAAT TTAAACAGAA TGGTGGTAAG
    CCCTATCTCT CTGTGATAAC GGGGAGAGGA AACCACAGCC AGGGAGGGGT TGCGCGCATC
    AAACCAGCCG TCATTAAATA CCTCACAAGC CATAACTTTA GATTCGCTGA AATTAAACCA
    GGGTGCTTGA AAGTCATGCT AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu23250
    Clone ID Related Accession (Same CDS sequence) XM_006068244.2 , XM_006068244.1
    Accession Version XM_006068244.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product NEDD4-binding protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785306.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCAGCCACT GATGCTAAGA TAGAAGAATC GTCTTCAACA 
    AACTTCGTTG CTTCTGAGAA ACAAGTAAAT GCAGTGAAAT GTGAAATAAT GGAAAAATGT
    CCTCCAGAAG AAGAGAGTGA AGATTTAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTAG ATACTTTAAT ACAAAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATAAC
    CAAGCATACT CATTTTTGCC TTTGCAAGAT GTTAATAATT TTAGTGACCC GAGTTCATTT
    GTAAGTTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CCCAAAATAT GGATTTGAAC
    AATGAAAATT TAGAGCATTC TGTCTCTGCA TTAAGTTCAA ATTCCTTAAC TTCACATTCA
    GTTTCAAATG GGTCCAAAAG TTTTACAAAA GATAACACAG TGGCATTGGA AGATTCTCTT
    CTCAGTAGTT CTTTAAATGC AGATAATGAC ACTTTGATGG ATGGCAGCAA CCAAATTCAG
    AAAGAGCTTT CGGAATCTGA GTGTGTTAAG ACTCAGTTCT CTCAAGCCCC TGTAGATTTG
    GATGCTAATG AGCTTCAGGC TCCTCTAAAC CTTGCTGTCC AAAATCCAGG GATTGGTTTA
    ATAGGTACAG GTGGAGATCA GAAGTCCACG TCTGTCCCTG ATGTTTTTGT ACCTTCTGAA
    GAATTCAGTT TCCAGCCACA CAAACATACT GAACTGCCAC CAAAGGGGAA GGATGTGAAT
    TACTGCCCAG TACTAACCCC TCTCCCTCTA CTGCTGCCTC CTCCCCCCCC TCCACCAATG
    TGGAATCCAA TGATTCCTGC TTTTGACCTC TTTCAAGCAA ACCATGGCTT TGTAGCTCCT
    GTTGTAACCA CAGCTGCACA CTGGAGGCCT GTCAATTATA CGTTTCCACC CCCAGTTATT
    TCTCATACTT CCCCAACAAA GGTATGGAGA AGTAAAGAGG GAACAAGTGC TTACCAGGTA
    CAAGAAACTC CAGTTTCTCA GGTTGTAAGA AAGAAGACAT CATCTTATGT TGGACTAGTT
    CTCGTTCTTC TCAGAGGCCT TCCAGGATCA GGAAAATCTT CTTTGGCAAG GACTTTGCAA
    GAGGATAATC CAAGTGGAGT TATTCTTAGT ACTGATGATT ATTTTTACAT AAATGGACAA
    TACCAGTTTG ATGTAAGATA CTTAGGAGAA GCACATGAAT GGAACCAGAA TCGTGCAAAA
    GAAGCATTTG AGAAGAAAGT GTCTCCTGTA ATAATAGATA ATACAAACCT ACAGGCATGG
    GAAATGAAGC CATATGTTGC TTTGTCTCAA AAACATAAAT ATAAAGTCCT TTTCCGGGAA
    CCGGATACAT GGTGGAAATT CAAACCGAAG GAACTTGCAA GGCGTAATAT TCATGGGATA
    AGCAAAGAAA AAATAACAAG AATGTTGGAA CACTATCAAC GTTTTGTTTC AGTGCCAATA
    ATCATGAGTT CTTCAGTTCC AGAGAAAATG GAACGCGTTG AATTGTGTGC ATATTCTTGT
    GACAGAAGTA CTAGCCCAAG AGACAATGAA GATATTACTT CTGAAAAAGA AGAAAATGTT
    TTATCCTCGT CTTTGAAGCA TCTAGAGTTA ACTGAAGAGA AGAAACTTGA TGTGACCAAA
    GAAAATATGT TACCTGAGAA TATTGCATAT CTCCCTAATG CAGATTTAAA CAAAGGAAAA
    AAAGAAATAA GTGATATGAA TCCCAATATT CAGAATGCTT TCATTCAGGA AGCTCCAAAC
    ACGTACTTTC CTGATTCTGA AAGTGAAGTA CAAGTTACAG ACAAAAGTGA AAAAGAAGAG
    CCAGTAGAAA TGGCTCTTGA AAAAGAGTGT AGTGAAACCA ATGCAGATAG TTTGTTAGAG
    AGAATGTCAC CAAGTAGTTG TTATGGTGGA AATAATCAAG GGGACCGTGG TCTTTCAACT
    CCTGTACCCA ATCAAAATGA AAAATCTTTA TCTAGTGAAA TATTGGAAGA AAGACCGGCA
    GTAAAGAAAA AAGCCTTTGG AAAACAAAAG GGCAAATCAA CTTTGGAAAA GTTTCCAAAA
    CACGAGCTGT CTTTTGTTGG TGACTGGCCA ATTGATAAGA CTATTGGTCA GAGGACAAAA
    AGGAACCGAA AAATGGAAAA AGCCTCATCT GTACAAAATG ACAAAAAGTA TAATCGCCCT
    CAGTCACATA AAATATTAGA TAATAGTTTA TCTGTGAGTG TAGATTGTAT GCAGCCACAA
    GGACCTCTAT ATGAAAATGT AGAGGATGAC GACAGGTCAC AGTGTGGTGA TGCCTCGGAA
    CCTTTCAGTA GCTGTGAATA TGATACTTAC AAAAACCCGG AAAGAGATTC ATTCAACATT
    GTGGGTGACT GGCCTTCCCT CGATTCGTTA GCTCAGAGAG AGCACAGATC AAGAATACCA
    AAGGCTGGCT TAAATGAACC CAGCCTAGAA TTTGGAACCA ACAACAGTAT GAATGAAATA
    TCCTTATACT CTTCACATGA GGCCTGTTGG GGCACAAGCC CTGAAGAGCT AAAAACATTG
    GGTGGTTGTA CTCGAGGAAG TTCTGAAATG TTGCCCAATG AAATGACCTA TGAGAGTAAG
    TCTTGTCCAA GTAAAAAGAT TCATAGGGAA CACACATTGT CTCTTCCTTA TATCAATAAT
    GTGCCAACTG TCCCTGGAGA AATAGGACCA CAAACTTTAG CTGAATTTTC AGAAGAAAAG
    ACTAAAGGAG TCCCTGGAAT AGGAGTAGGC ATGTGTACCC AGACTGAACC ACAGGATTTT
    GCTCTCTTAT GGAAAGTAGA AAAAAATAAG ATTAGTGTTT CTGATTCTCT GAGAGTATTA
    ACAGGAAGAT TAGATGGATT TAAACCCAAA ACTTTCAATA TTAACACAAA ATTGGATGTT
    CAAGAAGCAA TTCCATATAG GGTAATGTAT GACAAAAGCA CATATGTGGA AGAAAGTGAG
    CTCACCAGTG CTGATGAATC TGAAAATCTT AACATTCTTT GTAAACTATT TGGATCTTTT
    TCATTAGAAG CCCTAAAAGA CTTATATGAG AGGTGTAACA AAGATATTAT TTGGGCCACA
    AGCCTTTTAT TGGATTCTGA AACTAAATTA TGTGAGGATA CTGAATTTGA GAATATAAAA
    AAATCATACG ATGAAGCACA AATTGGGCCA TTTTCTCTGG GACTGAATTT GAAGGAAATT
    ATTAGCCAAA GAGGAAGCTT AGAGGATTCT AATTTTTCTG TGCCAGAATT TAGCAATGGA
    ATTGATATCA ATAACACTAA TGTACAGTCT ACCTGTAATT CCAAAAAGGA AAACTTAGAG
    CAGGCAGAAA TAAGAGCTAT ACCTACTGAA AAACCTAGAT TAATAGCAAG TATGCTTCCT
    ACTGTAGAGC TAAATAATAG TAATGAAGTA CTTCCTAATA GTCAAACAGA ACTTTCAGGT
    TTATATAATG TGAAGCAGTT TTTTCCAGAT ACTCTAAAAG CTACTACCAC TAAAACTGTG
    AATGAAATGG AAAAGAATTC AGAAGTTGCA GAGACTGGAG GTAACATACC ACCTTCTTTG
    AATTTGTCTG ACATTTTGAA CTCTGTATCA AGCACTTCAA ATCTTGAGTT ACATGAAGAA
    ACTTATTTTA CTAACTCTTT TGAAATAAAT AAAAATGAAA ATCTTCCAAA GGACTATGTG
    AAATTTCCAA ACACAGAAGA TTTTATGATT GAAGAGGAAC AGGAAATGGA GACAATTCTA
    ATGGCAGGGA GTGCTTTGTC AGCTGGAGTT ACTGGAGAAG ATAAAGCTGA GACACTGAAT
    CCCACTCCAG TGACAACCAA ATCTCTGACC ATAGACTGTC TTGAATTGGC ATTGCCCCCT
    GAACTGGCTT TTCAGCTCAA TGAATTATTT GGCCCTGTTG GTATTGATTC AGGGTCCCTA
    ACAGTTGAGG ATTGTGTGGT TCATATAGAT CTGAATCTGG CTAAAGTGAT TCATGAGAAA
    TGGAAAGAAT CTGTAATGGA GCGACAAAGA CAAGAAGAGG TATCTTGTGG CAAGTTAATG
    CAAGATCCTT CCCTGGTTGG GCATATTGGT CTTGATAATT CAGAACAAAA ATCATCTCAG
    AGAACAGGCA AAAAATTACT GAAGACTTTA GCAGCACCTG AAATGCTACC CTTATTGGAT
    CATTGGAACA CTCAGACTAA AAAAGTATCA CTCAGAGAAA TAATGTCAGA AGAAATTGCC
    TTACAGGAAA AGCATGATTT GAAAAGGGAG ACGCTTATGT TTGAGAAAGA TTGTGCCACT
    AAGCTAAAGG AGAAGCAACT CTTTAAGATA TTTCCAGCCA TTAACCAAAA TTTCCTGGTG
    GACATTTTCA AGGACCACAA CTATTCATTA GAACACACAG TGCAATTTCT AAATTGTGTT
    CTTGAAGGAG ACCCTGTAAA AACAGTGGTA GCACAAGAAT TTGTTCACCA AAATGAGAAT
    ATCACTTCTC ATACTGCACA GAAGTCTAAG GAGAAAAAGG CAAAGAAATT GAAAGAGACT
    GAAGATATAC CAAGTGAACC ATCTTTCCAG GACTTTGAGT ACCCAGAGTA TGATGACTAC
    AGGGCAGAGG CTTTCCTGCA CCAGCAGAAG AGGATGGAAT GCTACAGCAA GGCAAAAGAA
    GCTTATCGGA TGGGGAAGAA AAACGTTGCC ACATTTTATG CCCAGCAGGG TAGTCTCCAT
    GAGCAGAAGA TGAAAGAAGC TAATCACCTT GCTGCCGTGG AGATATTTGA AAAAGTCAAT
    GCCTCCCTAC TGCCGCAGAA TGTTCTAGAC CTCCATGGGC TGCATGTGGA TGAGGCTATA
    GAACATTTGA CAACAGTTTT ACAGCAGAAA ACTGAAGAAT TTAAACAGAA TGGTGGTAAG
    CCCTATCTCT CTGTGATAAC GGGGAGAGGA AACCACAGCC AGGGAGGGGT TGCGCGCATC
    AAACCAGCCG TCATTAAATA CCTCACAAGC CATAACTTTA GATTCGCTGA AATTAAACCA
    GGGTGCTTGA AAGTCATGCT AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006068306.1
    CDS194..5260
    Translation

    Target ORF information:

    RefSeq Version XM_006068244.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006068244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCAGCCACT GATGCTAAGA TAGAAGAATC GTCTTCAACA 
    AACTTCGTTG CTTCTGAGAA ACAAGTAAAT GCAGTGAAAT GTGAAATAAT GGAAAAATGT
    CCTCCAGAAG AAGAGAGTGA AGATTTAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTAG ATACTTTAAT ACAAAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATAAC
    CAAGCATACT CATTTTTGCC TTTGCAAGAT GTTAATAATT TTAGTGACCC GAGTTCATTT
    GTAAGTTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CCCAAAATAT GGATTTGAAC
    AATGAAAATT TAGAGCATTC TGTCTCTGCA TTAAGTTCAA ATTCCTTAAC TTCACATTCA
    GTTTCAAATG GGTCCAAAAG TTTTACAAAA GATAACACAG TGGCATTGGA AGATTCTCTT
    CTCAGTAGTT CTTTAAATGC AGATAATGAC ACTTTGATGG ATGGCAGCAA CCAAATTCAG
    AAAGAGCTTT CGGAATCTGA GTGTGTTAAG ACTCAGTTCT CTCAAGCCCC TGTAGATTTG
    GATGCTAATG AGCTTCAGGC TCCTCTAAAC CTTGCTGTCC AAAATCCAGG GATTGGTTTA
    ATAGGTACAG GTGGAGATCA GAAGTCCACG TCTGTCCCTG ATGTTTTTGT ACCTTCTGAA
    GAATTCAGTT TCCAGCCACA CAAACATACT GAACTGCCAC CAAAGGGGAA GGATGTGAAT
    TACTGCCCAG TACTAACCCC TCTCCCTCTA CTGCTGCCTC CTCCCCCCCC TCCACCAATG
    TGGAATCCAA TGATTCCTGC TTTTGACCTC TTTCAAGCAA ACCATGGCTT TGTAGCTCCT
    GTTGTAACCA CAGCTGCACA CTGGAGGCCT GTCAATTATA CGTTTCCACC CCCAGTTATT
    TCTCATACTT CCCCAACAAA GGTATGGAGA AGTAAAGAGG GAACAAGTGC TTACCAGGTA
    CAAGAAACTC CAGTTTCTCA GGTTGTAAGA AAGAAGACAT CATCTTATGT TGGACTAGTT
    CTCGTTCTTC TCAGAGGCCT TCCAGGATCA GGAAAATCTT CTTTGGCAAG GACTTTGCAA
    GAGGATAATC CAAGTGGAGT TATTCTTAGT ACTGATGATT ATTTTTACAT AAATGGACAA
    TACCAGTTTG ATGTAAGATA CTTAGGAGAA GCACATGAAT GGAACCAGAA TCGTGCAAAA
    GAAGCATTTG AGAAGAAAGT GTCTCCTGTA ATAATAGATA ATACAAACCT ACAGGCATGG
    GAAATGAAGC CATATGTTGC TTTGTCTCAA AAACATAAAT ATAAAGTCCT TTTCCGGGAA
    CCGGATACAT GGTGGAAATT CAAACCGAAG GAACTTGCAA GGCGTAATAT TCATGGGATA
    AGCAAAGAAA AAATAACAAG AATGTTGGAA CACTATCAAC GTTTTGTTTC AGTGCCAATA
    ATCATGAGTT CTTCAGTTCC AGAGAAAATG GAACGCGTTG AATTGTGTGC ATATTCTTGT
    GACAGAAGTA CTAGCCCAAG AGACAATGAA GATATTACTT CTGAAAAAGA AGAAAATGTT
    TTATCCTCGT CTTTGAAGCA TCTAGAGTTA ACTGAAGAGA AGAAACTTGA TGTGACCAAA
    GAAAATATGT TACCTGAGAA TATTGCATAT CTCCCTAATG CAGATTTAAA CAAAGGAAAA
    AAAGAAATAA GTGATATGAA TCCCAATATT CAGAATGCTT TCATTCAGGA AGCTCCAAAC
    ACGTACTTTC CTGATTCTGA AAGTGAAGTA CAAGTTACAG ACAAAAGTGA AAAAGAAGAG
    CCAGTAGAAA TGGCTCTTGA AAAAGAGTGT AGTGAAACCA ATGCAGATAG TTTGTTAGAG
    AGAATGTCAC CAAGTAGTTG TTATGGTGGA AATAATCAAG GGGACCGTGG TCTTTCAACT
    CCTGTACCCA ATCAAAATGA AAAATCTTTA TCTAGTGAAA TATTGGAAGA AAGACCGGCA
    GTAAAGAAAA AAGCCTTTGG AAAACAAAAG GGCAAATCAA CTTTGGAAAA GTTTCCAAAA
    CACGAGCTGT CTTTTGTTGG TGACTGGCCA ATTGATAAGA CTATTGGTCA GAGGACAAAA
    AGGAACCGAA AAATGGAAAA AGCCTCATCT GTACAAAATG ACAAAAAGTA TAATCGCCCT
    CAGTCACATA AAATATTAGA TAATAGTTTA TCTGTGAGTG TAGATTGTAT GCAGCCACAA
    GGACCTCTAT ATGAAAATGT AGAGGATGAC GACAGGTCAC AGTGTGGTGA TGCCTCGGAA
    CCTTTCAGTA GCTGTGAATA TGATACTTAC AAAAACCCGG AAAGAGATTC ATTCAACATT
    GTGGGTGACT GGCCTTCCCT CGATTCGTTA GCTCAGAGAG AGCACAGATC AAGAATACCA
    AAGGCTGGCT TAAATGAACC CAGCCTAGAA TTTGGAACCA ACAACAGTAT GAATGAAATA
    TCCTTATACT CTTCACATGA GGCCTGTTGG GGCACAAGCC CTGAAGAGCT AAAAACATTG
    GGTGGTTGTA CTCGAGGAAG TTCTGAAATG TTGCCCAATG AAATGACCTA TGAGAGTAAG
    TCTTGTCCAA GTAAAAAGAT TCATAGGGAA CACACATTGT CTCTTCCTTA TATCAATAAT
    GTGCCAACTG TCCCTGGAGA AATAGGACCA CAAACTTTAG CTGAATTTTC AGAAGAAAAG
    ACTAAAGGAG TCCCTGGAAT AGGAGTAGGC ATGTGTACCC AGACTGAACC ACAGGATTTT
    GCTCTCTTAT GGAAAGTAGA AAAAAATAAG ATTAGTGTTT CTGATTCTCT GAGAGTATTA
    ACAGGAAGAT TAGATGGATT TAAACCCAAA ACTTTCAATA TTAACACAAA ATTGGATGTT
    CAAGAAGCAA TTCCATATAG GGTAATGTAT GACAAAAGCA CATATGTGGA AGAAAGTGAG
    CTCACCAGTG CTGATGAATC TGAAAATCTT AACATTCTTT GTAAACTATT TGGATCTTTT
    TCATTAGAAG CCCTAAAAGA CTTATATGAG AGGTGTAACA AAGATATTAT TTGGGCCACA
    AGCCTTTTAT TGGATTCTGA AACTAAATTA TGTGAGGATA CTGAATTTGA GAATATAAAA
    AAATCATACG ATGAAGCACA AATTGGGCCA TTTTCTCTGG GACTGAATTT GAAGGAAATT
    ATTAGCCAAA GAGGAAGCTT AGAGGATTCT AATTTTTCTG TGCCAGAATT TAGCAATGGA
    ATTGATATCA ATAACACTAA TGTACAGTCT ACCTGTAATT CCAAAAAGGA AAACTTAGAG
    CAGGCAGAAA TAAGAGCTAT ACCTACTGAA AAACCTAGAT TAATAGCAAG TATGCTTCCT
    ACTGTAGAGC TAAATAATAG TAATGAAGTA CTTCCTAATA GTCAAACAGA ACTTTCAGGT
    TTATATAATG TGAAGCAGTT TTTTCCAGAT ACTCTAAAAG CTACTACCAC TAAAACTGTG
    AATGAAATGG AAAAGAATTC AGAAGTTGCA GAGACTGGAG GTAACATACC ACCTTCTTTG
    AATTTGTCTG ACATTTTGAA CTCTGTATCA AGCACTTCAA ATCTTGAGTT ACATGAAGAA
    ACTTATTTTA CTAACTCTTT TGAAATAAAT AAAAATGAAA ATCTTCCAAA GGACTATGTG
    AAATTTCCAA ACACAGAAGA TTTTATGATT GAAGAGGAAC AGGAAATGGA GACAATTCTA
    ATGGCAGGGA GTGCTTTGTC AGCTGGAGTT ACTGGAGAAG ATAAAGCTGA GACACTGAAT
    CCCACTCCAG TGACAACCAA ATCTCTGACC ATAGACTGTC TTGAATTGGC ATTGCCCCCT
    GAACTGGCTT TTCAGCTCAA TGAATTATTT GGCCCTGTTG GTATTGATTC AGGGTCCCTA
    ACAGTTGAGG ATTGTGTGGT TCATATAGAT CTGAATCTGG CTAAAGTGAT TCATGAGAAA
    TGGAAAGAAT CTGTAATGGA GCGACAAAGA CAAGAAGAGG TATCTTGTGG CAAGTTAATG
    CAAGATCCTT CCCTGGTTGG GCATATTGGT CTTGATAATT CAGAACAAAA ATCATCTCAG
    AGAACAGGCA AAAAATTACT GAAGACTTTA GCAGCACCTG AAATGCTACC CTTATTGGAT
    CATTGGAACA CTCAGACTAA AAAAGTATCA CTCAGAGAAA TAATGTCAGA AGAAATTGCC
    TTACAGGAAA AGCATGATTT GAAAAGGGAG ACGCTTATGT TTGAGAAAGA TTGTGCCACT
    AAGCTAAAGG AGAAGCAACT CTTTAAGATA TTTCCAGCCA TTAACCAAAA TTTCCTGGTG
    GACATTTTCA AGGACCACAA CTATTCATTA GAACACACAG TGCAATTTCT AAATTGTGTT
    CTTGAAGGAG ACCCTGTAAA AACAGTGGTA GCACAAGAAT TTGTTCACCA AAATGAGAAT
    ATCACTTCTC ATACTGCACA GAAGTCTAAG GAGAAAAAGG CAAAGAAATT GAAAGAGACT
    GAAGATATAC CAAGTGAACC ATCTTTCCAG GACTTTGAGT ACCCAGAGTA TGATGACTAC
    AGGGCAGAGG CTTTCCTGCA CCAGCAGAAG AGGATGGAAT GCTACAGCAA GGCAAAAGAA
    GCTTATCGGA TGGGGAAGAA AAACGTTGCC ACATTTTATG CCCAGCAGGG TAGTCTCCAT
    GAGCAGAAGA TGAAAGAAGC TAATCACCTT GCTGCCGTGG AGATATTTGA AAAAGTCAAT
    GCCTCCCTAC TGCCGCAGAA TGTTCTAGAC CTCCATGGGC TGCATGTGGA TGAGGCTATA
    GAACATTTGA CAACAGTTTT ACAGCAGAAA ACTGAAGAAT TTAAACAGAA TGGTGGTAAG
    CCCTATCTCT CTGTGATAAC GGGGAGAGGA AACCACAGCC AGGGAGGGGT TGCGCGCATC
    AAACCAGCCG TCATTAAATA CCTCACAAGC CATAACTTTA GATTCGCTGA AATTAAACCA
    GGGTGCTTGA AAGTCATGCT AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.