G2E3 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following G2E3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G2E3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu19581 XM_006063755.2
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu19582 XM_006063756.2
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu19583 XM_006063757.2
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBu56995 XM_025271541.1
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu56995 XM_025271539.1
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu56995 XM_025271540.1
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu56996 XM_025271542.1
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBu56997 XM_025271543.1
Latest version!
Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBu19581 XM_006063755.1 Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBu19583 XM_006063757.1 Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OBu19582 XM_006063756.1 Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu19581
    Clone ID Related Accession (Same CDS sequence) XM_006063755.2 , XM_006063755.1
    Accession Version XM_006063755.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006063755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGTTTTA AACAGGGTCT GAAAACTCTT GGTGTTTTGG AGAAAATTCA GACTTACCCA
    GAAGCATTTT TTCACATCTT CTGTCATAAA CCTGAGAATC TTTCTGCAAA AATACTTAGT
    GATCTTTTTA CAGTACACAC ATTGTCTGAT GTACAGACTT TGGGGTTTTG GAATAGTTAC
    TTACAGGCTG TTGAGGATGG TAAATCTACA GTAACAATGG AAGACATTCT TATTTTTGCT
    ACCGGTTGCA AGTCCATTCC ACCTGCTGGA TTTAAACCCA ATCCTTCAAT TGAGTGCCTT
    CATATGGATT TTCCTGTAGG AAACAAGTGT GATAACTGTT TAGCTCTTCC TATCACCAGT
    ACATATAAAG AATTTCAAGA AAGTATGGAC TTTGCCATAA AAAACACTCT AAGATTACAA
    AAAGAAGAAA ATTCTCACTT CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063817.1
    CDS125..2311
    Translation

    Target ORF information:

    RefSeq Version XM_006063755.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063755.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGTTTTA AACAGGGTCT GAAAACTCTT GGTGTTTTGG AGAAAATTCA GACTTACCCA
    GAAGCATTTT TTCACATCTT CTGTCATAAA CCTGAGAATC TTTCTGCAAA AATACTTAGT
    GATCTTTTTA CAGTACACAC ATTGTCTGAT GTACAGACTT TGGGGTTTTG GAATAGTTAC
    TTACAGGCTG TTGAGGATGG TAAATCTACA GTAACAATGG AAGACATTCT TATTTTTGCT
    ACCGGTTGCA AGTCCATTCC ACCTGCTGGA TTTAAACCCA ATCCTTCAAT TGAGTGCCTT
    CATATGGATT TTCCTGTAGG AAACAAGTGT GATAACTGTT TAGCTCTTCC TATCACCAGT
    ACATATAAAG AATTTCAAGA AAGTATGGAC TTTGCCATAA AAAACACTCT AAGATTACAA
    AAAGAAGAAA ATTCTCACTT CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu19581
    Clone ID Related Accession (Same CDS sequence) XM_006063755.2 , XM_006063755.1
    Accession Version XM_006063755.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785113.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGTTTTA AACAGGGTCT GAAAACTCTT GGTGTTTTGG AGAAAATTCA GACTTACCCA
    GAAGCATTTT TTCACATCTT CTGTCATAAA CCTGAGAATC TTTCTGCAAA AATACTTAGT
    GATCTTTTTA CAGTACACAC ATTGTCTGAT GTACAGACTT TGGGGTTTTG GAATAGTTAC
    TTACAGGCTG TTGAGGATGG TAAATCTACA GTAACAATGG AAGACATTCT TATTTTTGCT
    ACCGGTTGCA AGTCCATTCC ACCTGCTGGA TTTAAACCCA ATCCTTCAAT TGAGTGCCTT
    CATATGGATT TTCCTGTAGG AAACAAGTGT GATAACTGTT TAGCTCTTCC TATCACCAGT
    ACATATAAAG AATTTCAAGA AAGTATGGAC TTTGCCATAA AAAACACTCT AAGATTACAA
    AAAGAAGAAA ATTCTCACTT CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063817.1
    CDS366..2552
    Translation

    Target ORF information:

    RefSeq Version XM_006063755.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGTTTTA AACAGGGTCT GAAAACTCTT GGTGTTTTGG AGAAAATTCA GACTTACCCA
    GAAGCATTTT TTCACATCTT CTGTCATAAA CCTGAGAATC TTTCTGCAAA AATACTTAGT
    GATCTTTTTA CAGTACACAC ATTGTCTGAT GTACAGACTT TGGGGTTTTG GAATAGTTAC
    TTACAGGCTG TTGAGGATGG TAAATCTACA GTAACAATGG AAGACATTCT TATTTTTGCT
    ACCGGTTGCA AGTCCATTCC ACCTGCTGGA TTTAAACCCA ATCCTTCAAT TGAGTGCCTT
    CATATGGATT TTCCTGTAGG AAACAAGTGT GATAACTGTT TAGCTCTTCC TATCACCAGT
    ACATATAAAG AATTTCAAGA AAGTATGGAC TTTGCCATAA AAAACACTCT AAGATTACAA
    AAAGAAGAAA ATTCTCACTT CATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu19582
    Clone ID Related Accession (Same CDS sequence) XM_006063756.1 , XM_006063756.2
    Accession Version XM_006063756.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785113.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG AAATGCTCTG TTTGCAAGAA AATGGGTGCT
    TCAATTGGGT GTGTCGCACC CCGATGTAAA CGAAGTTATC ATTTCCCATG TGGACTTCAG
    AGAGAATGTA TTTTCCAGTT CACTGGCAAT TTTGCGTCAT TTTGTTGGCA ACATCGACCT
    GTTCAAATAA TTACATCTAA TAATTGTAGA GACTCCTTAC TGTGCACCAT TTGCTTGGAA
    TTTATAGAGC CTATTCCAAC TTACAGCGTG TTACGAAGTC CTTGTTGTAA GAATGCTTGG
    TTTCATAGAG ACTGTTTGCA GGTGCAAGCA ATAAATGCAG GAATGTTTTT CTTTAGGTGC
    ACTATATGCA ATAATAGTGA TATATTTCAG AAAGAGATGT TGAGAATGGG AATTCATATT
    CCTGAAAAAG ATGCATCTTG GGAATTAGAG GAAAATGCTT ATCAAGAGCT TCTGCAGCAC
    TATGAGCATT GTGATGTCCG AAGATGTCGT TGCAAAGAAG GGCGAGACTA TAATGTGCCT
    GATAGCAAAT GGGAAATAAA GCGCTGTCAG TGTTGTGGTT CTAGCGGAAC ACATTTAGCC
    TGTTCCTCAC TACAGTCATG GGAACAAAAT TGGGAATGTT TGGAATGTAG AAGTATTCTC
    TACAGTGCAG GCGAATTCCA GAAAGCCAAA AAACATGCAT TACCCAACAC TAATGTGGGA
    ATAAATGATT GTTTGTTGGA GGAGTCTTCA CCTAAATTAC CCAGACAGTC ACCTGGAGAC
    CAGTGTAAAG ATCTGCTGAG GCAAGGCAGC AAATTTAGAA GAGATATCTC GACTCTAATT
    ATAGAGTTAG CATTCCAAGT TAAGAAAAGG CCTAAAAAAG TATTTATCAA CAAAGCCAAT
    TTCTGGAATA GTGCCTTAGA TGGATTCAGA AATCAAAACT TTAATCCTTC ATATGCAATT
    GAAATAGTGT ATGTTAATGA AAATGATCAT TTTGGAAGAG AGCAGCCTGG ATCAAAGCAA
    GAATTCCTAA GTCTCTTAAT GCAGCAACTT GAGAACTCAC CATTATTTGA AGGGTCCTTA
    TCAAAGAACT TGTCTTTAAA TTCTCAAGCT CTGAAAGAGA ACCTTTACTA TGAAGCTGGC
    AAAATGGTTG CCATTTCTTT GGTTCATGGT GGTCCTTCAC CTGGTTTCTT TTCTCAGACC
    TTATTTAACT GCCTTGCTTA TGGACCAGAA AATACCCAAC CAATTTTAGA CGATGTTTCA
    GACTTTGATG TGGCACAGAT TATGATCAGG GTCAATACTG CAGCAACTGT GGCTGACTTA
    AACTCTTTAG TACATGACTA TTATAAGTAC TTAGAGCTTA TTGGATGTCT AAGACTTATA
    ACATCGATAA GTGATAAATA TATGTTGGCA AAAGACATAC TTGTTTATCA TGTAATTAAA
    AGAGTCCAAG CACCTTTTGA GAGTTTTAAA CAGGGTCTGA AAACTCTTGG TGTTTTGGAG
    AAAATTCAGA CTTACCCAGA AGCATTTTTT CACATCTTCT GTCATAAACC TGAGAATCTT
    TCTGCAAAAA TACTTAGTGA TCTTTTTACA GTACACACAT TGTCTGATGT ACAGACTTTG
    GGGTTTTGGA ATAGTTACTT ACAGGCTGTT GAGGATGGTA AATCTACAGT AACAATGGAA
    GACATTCTTA TTTTTGCTAC CGGTTGCAAG TCCATTCCAC CTGCTGGATT TAAACCCAAT
    CCTTCAATTG AGTGCCTTCA TATGGATTTT CCTGTAGGAA ACAAGTGTGA TAACTGTTTA
    GCTCTTCCTA TCACCAGTAC ATATAAAGAA TTTCAAGAAA GTATGGACTT TGCCATAAAA
    AACACTCTAA GATTACAAAA AGAAGAAAAT TCTCACTTCA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063818.1
    CDS366..2450
    Translation

    Target ORF information:

    RefSeq Version XM_006063756.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG AAATGCTCTG TTTGCAAGAA AATGGGTGCT
    TCAATTGGGT GTGTCGCACC CCGATGTAAA CGAAGTTATC ATTTCCCATG TGGACTTCAG
    AGAGAATGTA TTTTCCAGTT CACTGGCAAT TTTGCGTCAT TTTGTTGGCA ACATCGACCT
    GTTCAAATAA TTACATCTAA TAATTGTAGA GACTCCTTAC TGTGCACCAT TTGCTTGGAA
    TTTATAGAGC CTATTCCAAC TTACAGCGTG TTACGAAGTC CTTGTTGTAA GAATGCTTGG
    TTTCATAGAG ACTGTTTGCA GGTGCAAGCA ATAAATGCAG GAATGTTTTT CTTTAGGTGC
    ACTATATGCA ATAATAGTGA TATATTTCAG AAAGAGATGT TGAGAATGGG AATTCATATT
    CCTGAAAAAG ATGCATCTTG GGAATTAGAG GAAAATGCTT ATCAAGAGCT TCTGCAGCAC
    TATGAGCATT GTGATGTCCG AAGATGTCGT TGCAAAGAAG GGCGAGACTA TAATGTGCCT
    GATAGCAAAT GGGAAATAAA GCGCTGTCAG TGTTGTGGTT CTAGCGGAAC ACATTTAGCC
    TGTTCCTCAC TACAGTCATG GGAACAAAAT TGGGAATGTT TGGAATGTAG AAGTATTCTC
    TACAGTGCAG GCGAATTCCA GAAAGCCAAA AAACATGCAT TACCCAACAC TAATGTGGGA
    ATAAATGATT GTTTGTTGGA GGAGTCTTCA CCTAAATTAC CCAGACAGTC ACCTGGAGAC
    CAGTGTAAAG ATCTGCTGAG GCAAGGCAGC AAATTTAGAA GAGATATCTC GACTCTAATT
    ATAGAGTTAG CATTCCAAGT TAAGAAAAGG CCTAAAAAAG TATTTATCAA CAAAGCCAAT
    TTCTGGAATA GTGCCTTAGA TGGATTCAGA AATCAAAACT TTAATCCTTC ATATGCAATT
    GAAATAGTGT ATGTTAATGA AAATGATCAT TTTGGAAGAG AGCAGCCTGG ATCAAAGCAA
    GAATTCCTAA GTCTCTTAAT GCAGCAACTT GAGAACTCAC CATTATTTGA AGGGTCCTTA
    TCAAAGAACT TGTCTTTAAA TTCTCAAGCT CTGAAAGAGA ACCTTTACTA TGAAGCTGGC
    AAAATGGTTG CCATTTCTTT GGTTCATGGT GGTCCTTCAC CTGGTTTCTT TTCTCAGACC
    TTATTTAACT GCCTTGCTTA TGGACCAGAA AATACCCAAC CAATTTTAGA CGATGTTTCA
    GACTTTGATG TGGCACAGAT TATGATCAGG GTCAATACTG CAGCAACTGT GGCTGACTTA
    AACTCTTTAG TACATGACTA TTATAAGTAC TTAGAGCTTA TTGGATGTCT AAGACTTATA
    ACATCGATAA GTGATAAATA TATGTTGGCA AAAGACATAC TTGTTTATCA TGTAATTAAA
    AGAGTCCAAG CACCTTTTGA GAGTTTTAAA CAGGGTCTGA AAACTCTTGG TGTTTTGGAG
    AAAATTCAGA CTTACCCAGA AGCATTTTTT CACATCTTCT GTCATAAACC TGAGAATCTT
    TCTGCAAAAA TACTTAGTGA TCTTTTTACA GTACACACAT TGTCTGATGT ACAGACTTTG
    GGGTTTTGGA ATAGTTACTT ACAGGCTGTT GAGGATGGTA AATCTACAGT AACAATGGAA
    GACATTCTTA TTTTTGCTAC CGGTTGCAAG TCCATTCCAC CTGCTGGATT TAAACCCAAT
    CCTTCAATTG AGTGCCTTCA TATGGATTTT CCTGTAGGAA ACAAGTGTGA TAACTGTTTA
    GCTCTTCCTA TCACCAGTAC ATATAAAGAA TTTCAAGAAA GTATGGACTT TGCCATAAAA
    AACACTCTAA GATTACAAAA AGAAGAAAAT TCTCACTTCA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu19582
    Clone ID Related Accession (Same CDS sequence) XM_006063756.1 , XM_006063756.2
    Accession Version XM_006063756.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006063756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG AAATGCTCTG TTTGCAAGAA AATGGGTGCT
    TCAATTGGGT GTGTCGCACC CCGATGTAAA CGAAGTTATC ATTTCCCATG TGGACTTCAG
    AGAGAATGTA TTTTCCAGTT CACTGGCAAT TTTGCGTCAT TTTGTTGGCA ACATCGACCT
    GTTCAAATAA TTACATCTAA TAATTGTAGA GACTCCTTAC TGTGCACCAT TTGCTTGGAA
    TTTATAGAGC CTATTCCAAC TTACAGCGTG TTACGAAGTC CTTGTTGTAA GAATGCTTGG
    TTTCATAGAG ACTGTTTGCA GGTGCAAGCA ATAAATGCAG GAATGTTTTT CTTTAGGTGC
    ACTATATGCA ATAATAGTGA TATATTTCAG AAAGAGATGT TGAGAATGGG AATTCATATT
    CCTGAAAAAG ATGCATCTTG GGAATTAGAG GAAAATGCTT ATCAAGAGCT TCTGCAGCAC
    TATGAGCATT GTGATGTCCG AAGATGTCGT TGCAAAGAAG GGCGAGACTA TAATGTGCCT
    GATAGCAAAT GGGAAATAAA GCGCTGTCAG TGTTGTGGTT CTAGCGGAAC ACATTTAGCC
    TGTTCCTCAC TACAGTCATG GGAACAAAAT TGGGAATGTT TGGAATGTAG AAGTATTCTC
    TACAGTGCAG GCGAATTCCA GAAAGCCAAA AAACATGCAT TACCCAACAC TAATGTGGGA
    ATAAATGATT GTTTGTTGGA GGAGTCTTCA CCTAAATTAC CCAGACAGTC ACCTGGAGAC
    CAGTGTAAAG ATCTGCTGAG GCAAGGCAGC AAATTTAGAA GAGATATCTC GACTCTAATT
    ATAGAGTTAG CATTCCAAGT TAAGAAAAGG CCTAAAAAAG TATTTATCAA CAAAGCCAAT
    TTCTGGAATA GTGCCTTAGA TGGATTCAGA AATCAAAACT TTAATCCTTC ATATGCAATT
    GAAATAGTGT ATGTTAATGA AAATGATCAT TTTGGAAGAG AGCAGCCTGG ATCAAAGCAA
    GAATTCCTAA GTCTCTTAAT GCAGCAACTT GAGAACTCAC CATTATTTGA AGGGTCCTTA
    TCAAAGAACT TGTCTTTAAA TTCTCAAGCT CTGAAAGAGA ACCTTTACTA TGAAGCTGGC
    AAAATGGTTG CCATTTCTTT GGTTCATGGT GGTCCTTCAC CTGGTTTCTT TTCTCAGACC
    TTATTTAACT GCCTTGCTTA TGGACCAGAA AATACCCAAC CAATTTTAGA CGATGTTTCA
    GACTTTGATG TGGCACAGAT TATGATCAGG GTCAATACTG CAGCAACTGT GGCTGACTTA
    AACTCTTTAG TACATGACTA TTATAAGTAC TTAGAGCTTA TTGGATGTCT AAGACTTATA
    ACATCGATAA GTGATAAATA TATGTTGGCA AAAGACATAC TTGTTTATCA TGTAATTAAA
    AGAGTCCAAG CACCTTTTGA GAGTTTTAAA CAGGGTCTGA AAACTCTTGG TGTTTTGGAG
    AAAATTCAGA CTTACCCAGA AGCATTTTTT CACATCTTCT GTCATAAACC TGAGAATCTT
    TCTGCAAAAA TACTTAGTGA TCTTTTTACA GTACACACAT TGTCTGATGT ACAGACTTTG
    GGGTTTTGGA ATAGTTACTT ACAGGCTGTT GAGGATGGTA AATCTACAGT AACAATGGAA
    GACATTCTTA TTTTTGCTAC CGGTTGCAAG TCCATTCCAC CTGCTGGATT TAAACCCAAT
    CCTTCAATTG AGTGCCTTCA TATGGATTTT CCTGTAGGAA ACAAGTGTGA TAACTGTTTA
    GCTCTTCCTA TCACCAGTAC ATATAAAGAA TTTCAAGAAA GTATGGACTT TGCCATAAAA
    AACACTCTAA GATTACAAAA AGAAGAAAAT TCTCACTTCA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063818.1
    CDS125..2209
    Translation

    Target ORF information:

    RefSeq Version XM_006063756.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063756.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG AAATGCTCTG TTTGCAAGAA AATGGGTGCT
    TCAATTGGGT GTGTCGCACC CCGATGTAAA CGAAGTTATC ATTTCCCATG TGGACTTCAG
    AGAGAATGTA TTTTCCAGTT CACTGGCAAT TTTGCGTCAT TTTGTTGGCA ACATCGACCT
    GTTCAAATAA TTACATCTAA TAATTGTAGA GACTCCTTAC TGTGCACCAT TTGCTTGGAA
    TTTATAGAGC CTATTCCAAC TTACAGCGTG TTACGAAGTC CTTGTTGTAA GAATGCTTGG
    TTTCATAGAG ACTGTTTGCA GGTGCAAGCA ATAAATGCAG GAATGTTTTT CTTTAGGTGC
    ACTATATGCA ATAATAGTGA TATATTTCAG AAAGAGATGT TGAGAATGGG AATTCATATT
    CCTGAAAAAG ATGCATCTTG GGAATTAGAG GAAAATGCTT ATCAAGAGCT TCTGCAGCAC
    TATGAGCATT GTGATGTCCG AAGATGTCGT TGCAAAGAAG GGCGAGACTA TAATGTGCCT
    GATAGCAAAT GGGAAATAAA GCGCTGTCAG TGTTGTGGTT CTAGCGGAAC ACATTTAGCC
    TGTTCCTCAC TACAGTCATG GGAACAAAAT TGGGAATGTT TGGAATGTAG AAGTATTCTC
    TACAGTGCAG GCGAATTCCA GAAAGCCAAA AAACATGCAT TACCCAACAC TAATGTGGGA
    ATAAATGATT GTTTGTTGGA GGAGTCTTCA CCTAAATTAC CCAGACAGTC ACCTGGAGAC
    CAGTGTAAAG ATCTGCTGAG GCAAGGCAGC AAATTTAGAA GAGATATCTC GACTCTAATT
    ATAGAGTTAG CATTCCAAGT TAAGAAAAGG CCTAAAAAAG TATTTATCAA CAAAGCCAAT
    TTCTGGAATA GTGCCTTAGA TGGATTCAGA AATCAAAACT TTAATCCTTC ATATGCAATT
    GAAATAGTGT ATGTTAATGA AAATGATCAT TTTGGAAGAG AGCAGCCTGG ATCAAAGCAA
    GAATTCCTAA GTCTCTTAAT GCAGCAACTT GAGAACTCAC CATTATTTGA AGGGTCCTTA
    TCAAAGAACT TGTCTTTAAA TTCTCAAGCT CTGAAAGAGA ACCTTTACTA TGAAGCTGGC
    AAAATGGTTG CCATTTCTTT GGTTCATGGT GGTCCTTCAC CTGGTTTCTT TTCTCAGACC
    TTATTTAACT GCCTTGCTTA TGGACCAGAA AATACCCAAC CAATTTTAGA CGATGTTTCA
    GACTTTGATG TGGCACAGAT TATGATCAGG GTCAATACTG CAGCAACTGT GGCTGACTTA
    AACTCTTTAG TACATGACTA TTATAAGTAC TTAGAGCTTA TTGGATGTCT AAGACTTATA
    ACATCGATAA GTGATAAATA TATGTTGGCA AAAGACATAC TTGTTTATCA TGTAATTAAA
    AGAGTCCAAG CACCTTTTGA GAGTTTTAAA CAGGGTCTGA AAACTCTTGG TGTTTTGGAG
    AAAATTCAGA CTTACCCAGA AGCATTTTTT CACATCTTCT GTCATAAACC TGAGAATCTT
    TCTGCAAAAA TACTTAGTGA TCTTTTTACA GTACACACAT TGTCTGATGT ACAGACTTTG
    GGGTTTTGGA ATAGTTACTT ACAGGCTGTT GAGGATGGTA AATCTACAGT AACAATGGAA
    GACATTCTTA TTTTTGCTAC CGGTTGCAAG TCCATTCCAC CTGCTGGATT TAAACCCAAT
    CCTTCAATTG AGTGCCTTCA TATGGATTTT CCTGTAGGAA ACAAGTGTGA TAACTGTTTA
    GCTCTTCCTA TCACCAGTAC ATATAAAGAA TTTCAAGAAA GTATGGACTT TGCCATAAAA
    AACACTCTAA GATTACAAAA AGAAGAAAAT TCTCACTTCA TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu19583
    Clone ID Related Accession (Same CDS sequence) XM_006063757.2 , XM_006063757.1
    Accession Version XM_006063757.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006063757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTAAAATGCT CTGTTTGCAA GAAAATGGGT 
    GCTTCAATTG GGTGTGTCGC ACCCCGATGT AAACGAAGTT ATCATTTCCC ATGTGGACTT
    CAGAGAGAAT GTATTTTCCA GTTCACTGGC AATTTTGCGT CATTTTGTTG GCAACATCGA
    CCTGTTCAAA TAATTACATC TAATAATTGT AGAGACTCCT TACTGTGCAC CATTTGCTTG
    GAATTTATAG AGCCTATTCC AACTTACAGC GTGTTACGAA GTCCTTGTTG TAAGAATGCT
    TGGTTTCATA GAGACTGTTT GCAGGTGCAA GCAATAAATG CAGGAATGTT TTTCTTTAGG
    TGCACTATAT GCAATAATAG TGATATATTT CAGAAAGAGA TGTTGAGAAT GGGAATTCAT
    ATTCCTGAAA AAGATGCATC TTGGGAATTA GAGGAAAATG CTTATCAAGA GCTTCTGCAG
    CACTATGAGC ATTGTGATGT CCGAAGATGT CGTTGCAAAG AAGGGCGAGA CTATAATGTG
    CCTGATAGCA AATGGGAAAT AAAGCGCTGT CAGTGTTGTG GTTCTAGCGG AACACATTTA
    GCCTGTTCCT CACTACAGTC ATGGGAACAA AATTGGGAAT GTTTGGAATG TAGAAGTATT
    CTCTACAGTG CAGGCGAATT CCAGAAAGCC AAAAAACATG CATTACCCAA CACTAATGTG
    GGAATAAATG ATTGTTTGTT GGAGGAGTCT TCACCTAAAT TACCCAGACA GTCACCTGGA
    GACCAGTGTA AAGATCTGCT GAGGCAAGGC AGCAAATTTA GAAGAGATAT CTCGACTCTA
    ATTATAGAGT TAGCATTCCA AGTTAAGAAA AGGCCTAAAA AAGTATTTAT CAACAAAGCC
    AATTTCTGGA ATAGTGCCTT AGATGGATTC AGAAATCAAA ACTTTAATCC TTCATATGCA
    ATTGAAATAG TGTATGTTAA TGAAAATGAT CATTTTGGAA GAGAGCAGCC TGGATCAAAG
    CAAGAATTCC TAAGTCTCTT AATGCAGCAA CTTGAGAACT CACCATTATT TGAAGGGTCC
    TTATCAAAGA ACTTGTCTTT AAATTCTCAA GCTCTGAAAG AGAACCTTTA CTATGAAGCT
    GGCAAAATGG TTGCCATTTC TTTGGTTCAT GGTGGTCCTT CACCTGGTTT CTTTTCTCAG
    ACCTTATTTA ACTGCCTTGC TTATGGACCA GAAAATACCC AACCAATTTT AGACGATGTT
    TCAGACTTTG ATGTGGCACA GATTATGATC AGGGTCAATA CTGCAGCAAC TGTGGCTGAC
    TTAAACTCTT TAGTACATGA CTATTATAAG TACTTAGAGC TTATTGGATG TCTAAGACTT
    ATAACATCGA TAAGTGATAA ATATATGTTG GCAAAAGACA TACTTGTTTA TCATGTAATT
    AAAAGAGTCC AAGCACCTTT TGAGAGTTTT AAACAGGGTC TGAAAACTCT TGGTGTTTTG
    GAGAAAATTC AGACTTACCC AGAAGCATTT TTTCACATCT TCTGTCATAA ACCTGAGAAT
    CTTTCTGCAA AAATACTTAG TGATCTTTTT ACAGTACACA CATTGTCTGA TGTACAGACT
    TTGGGGTTTT GGAATAGTTA CTTACAGGCT GTTGAGGATG GTAAATCTAC AGTAACAATG
    GAAGACATTC TTATTTTTGC TACCGGTTGC AAGTCCATTC CACCTGCTGG ATTTAAACCC
    AATCCTTCAA TTGAGTGCCT TCATATGGAT TTTCCTGTAG GAAACAAGTG TGATAACTGT
    TTAGCTCTTC CTATCACCAG TACATATAAA GAATTTCAAG AAAGTATGGA CTTTGCCATA
    AAAAACACTC TAAGATTACA AAAAGAAGAA AATTCTCACT TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063819.1
    CDS143..2050
    Translation

    Target ORF information:

    RefSeq Version XM_006063757.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063757.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTAAAATGCT CTGTTTGCAA GAAAATGGGT 
    GCTTCAATTG GGTGTGTCGC ACCCCGATGT AAACGAAGTT ATCATTTCCC ATGTGGACTT
    CAGAGAGAAT GTATTTTCCA GTTCACTGGC AATTTTGCGT CATTTTGTTG GCAACATCGA
    CCTGTTCAAA TAATTACATC TAATAATTGT AGAGACTCCT TACTGTGCAC CATTTGCTTG
    GAATTTATAG AGCCTATTCC AACTTACAGC GTGTTACGAA GTCCTTGTTG TAAGAATGCT
    TGGTTTCATA GAGACTGTTT GCAGGTGCAA GCAATAAATG CAGGAATGTT TTTCTTTAGG
    TGCACTATAT GCAATAATAG TGATATATTT CAGAAAGAGA TGTTGAGAAT GGGAATTCAT
    ATTCCTGAAA AAGATGCATC TTGGGAATTA GAGGAAAATG CTTATCAAGA GCTTCTGCAG
    CACTATGAGC ATTGTGATGT CCGAAGATGT CGTTGCAAAG AAGGGCGAGA CTATAATGTG
    CCTGATAGCA AATGGGAAAT AAAGCGCTGT CAGTGTTGTG GTTCTAGCGG AACACATTTA
    GCCTGTTCCT CACTACAGTC ATGGGAACAA AATTGGGAAT GTTTGGAATG TAGAAGTATT
    CTCTACAGTG CAGGCGAATT CCAGAAAGCC AAAAAACATG CATTACCCAA CACTAATGTG
    GGAATAAATG ATTGTTTGTT GGAGGAGTCT TCACCTAAAT TACCCAGACA GTCACCTGGA
    GACCAGTGTA AAGATCTGCT GAGGCAAGGC AGCAAATTTA GAAGAGATAT CTCGACTCTA
    ATTATAGAGT TAGCATTCCA AGTTAAGAAA AGGCCTAAAA AAGTATTTAT CAACAAAGCC
    AATTTCTGGA ATAGTGCCTT AGATGGATTC AGAAATCAAA ACTTTAATCC TTCATATGCA
    ATTGAAATAG TGTATGTTAA TGAAAATGAT CATTTTGGAA GAGAGCAGCC TGGATCAAAG
    CAAGAATTCC TAAGTCTCTT AATGCAGCAA CTTGAGAACT CACCATTATT TGAAGGGTCC
    TTATCAAAGA ACTTGTCTTT AAATTCTCAA GCTCTGAAAG AGAACCTTTA CTATGAAGCT
    GGCAAAATGG TTGCCATTTC TTTGGTTCAT GGTGGTCCTT CACCTGGTTT CTTTTCTCAG
    ACCTTATTTA ACTGCCTTGC TTATGGACCA GAAAATACCC AACCAATTTT AGACGATGTT
    TCAGACTTTG ATGTGGCACA GATTATGATC AGGGTCAATA CTGCAGCAAC TGTGGCTGAC
    TTAAACTCTT TAGTACATGA CTATTATAAG TACTTAGAGC TTATTGGATG TCTAAGACTT
    ATAACATCGA TAAGTGATAA ATATATGTTG GCAAAAGACA TACTTGTTTA TCATGTAATT
    AAAAGAGTCC AAGCACCTTT TGAGAGTTTT AAACAGGGTC TGAAAACTCT TGGTGTTTTG
    GAGAAAATTC AGACTTACCC AGAAGCATTT TTTCACATCT TCTGTCATAA ACCTGAGAAT
    CTTTCTGCAA AAATACTTAG TGATCTTTTT ACAGTACACA CATTGTCTGA TGTACAGACT
    TTGGGGTTTT GGAATAGTTA CTTACAGGCT GTTGAGGATG GTAAATCTAC AGTAACAATG
    GAAGACATTC TTATTTTTGC TACCGGTTGC AAGTCCATTC CACCTGCTGG ATTTAAACCC
    AATCCTTCAA TTGAGTGCCT TCATATGGAT TTTCCTGTAG GAAACAAGTG TGATAACTGT
    TTAGCTCTTC CTATCACCAG TACATATAAA GAATTTCAAG AAAGTATGGA CTTTGCCATA
    AAAAACACTC TAAGATTACA AAAAGAAGAA AATTCTCACT TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu19583
    Clone ID Related Accession (Same CDS sequence) XM_006063757.2 , XM_006063757.1
    Accession Version XM_006063757.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785113.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTAAAATGCT CTGTTTGCAA GAAAATGGGT 
    GCTTCAATTG GGTGTGTCGC ACCCCGATGT AAACGAAGTT ATCATTTCCC ATGTGGACTT
    CAGAGAGAAT GTATTTTCCA GTTCACTGGC AATTTTGCGT CATTTTGTTG GCAACATCGA
    CCTGTTCAAA TAATTACATC TAATAATTGT AGAGACTCCT TACTGTGCAC CATTTGCTTG
    GAATTTATAG AGCCTATTCC AACTTACAGC GTGTTACGAA GTCCTTGTTG TAAGAATGCT
    TGGTTTCATA GAGACTGTTT GCAGGTGCAA GCAATAAATG CAGGAATGTT TTTCTTTAGG
    TGCACTATAT GCAATAATAG TGATATATTT CAGAAAGAGA TGTTGAGAAT GGGAATTCAT
    ATTCCTGAAA AAGATGCATC TTGGGAATTA GAGGAAAATG CTTATCAAGA GCTTCTGCAG
    CACTATGAGC ATTGTGATGT CCGAAGATGT CGTTGCAAAG AAGGGCGAGA CTATAATGTG
    CCTGATAGCA AATGGGAAAT AAAGCGCTGT CAGTGTTGTG GTTCTAGCGG AACACATTTA
    GCCTGTTCCT CACTACAGTC ATGGGAACAA AATTGGGAAT GTTTGGAATG TAGAAGTATT
    CTCTACAGTG CAGGCGAATT CCAGAAAGCC AAAAAACATG CATTACCCAA CACTAATGTG
    GGAATAAATG ATTGTTTGTT GGAGGAGTCT TCACCTAAAT TACCCAGACA GTCACCTGGA
    GACCAGTGTA AAGATCTGCT GAGGCAAGGC AGCAAATTTA GAAGAGATAT CTCGACTCTA
    ATTATAGAGT TAGCATTCCA AGTTAAGAAA AGGCCTAAAA AAGTATTTAT CAACAAAGCC
    AATTTCTGGA ATAGTGCCTT AGATGGATTC AGAAATCAAA ACTTTAATCC TTCATATGCA
    ATTGAAATAG TGTATGTTAA TGAAAATGAT CATTTTGGAA GAGAGCAGCC TGGATCAAAG
    CAAGAATTCC TAAGTCTCTT AATGCAGCAA CTTGAGAACT CACCATTATT TGAAGGGTCC
    TTATCAAAGA ACTTGTCTTT AAATTCTCAA GCTCTGAAAG AGAACCTTTA CTATGAAGCT
    GGCAAAATGG TTGCCATTTC TTTGGTTCAT GGTGGTCCTT CACCTGGTTT CTTTTCTCAG
    ACCTTATTTA ACTGCCTTGC TTATGGACCA GAAAATACCC AACCAATTTT AGACGATGTT
    TCAGACTTTG ATGTGGCACA GATTATGATC AGGGTCAATA CTGCAGCAAC TGTGGCTGAC
    TTAAACTCTT TAGTACATGA CTATTATAAG TACTTAGAGC TTATTGGATG TCTAAGACTT
    ATAACATCGA TAAGTGATAA ATATATGTTG GCAAAAGACA TACTTGTTTA TCATGTAATT
    AAAAGAGTCC AAGCACCTTT TGAGAGTTTT AAACAGGGTC TGAAAACTCT TGGTGTTTTG
    GAGAAAATTC AGACTTACCC AGAAGCATTT TTTCACATCT TCTGTCATAA ACCTGAGAAT
    CTTTCTGCAA AAATACTTAG TGATCTTTTT ACAGTACACA CATTGTCTGA TGTACAGACT
    TTGGGGTTTT GGAATAGTTA CTTACAGGCT GTTGAGGATG GTAAATCTAC AGTAACAATG
    GAAGACATTC TTATTTTTGC TACCGGTTGC AAGTCCATTC CACCTGCTGG ATTTAAACCC
    AATCCTTCAA TTGAGTGCCT TCATATGGAT TTTCCTGTAG GAAACAAGTG TGATAACTGT
    TTAGCTCTTC CTATCACCAG TACATATAAA GAATTTCAAG AAAGTATGGA CTTTGCCATA
    AAAAACACTC TAAGATTACA AAAAGAAGAA AATTCTCACT TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006063819.1
    CDS56..1963
    Translation

    Target ORF information:

    RefSeq Version XM_006063757.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006063757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTAAAATGCT CTGTTTGCAA GAAAATGGGT 
    GCTTCAATTG GGTGTGTCGC ACCCCGATGT AAACGAAGTT ATCATTTCCC ATGTGGACTT
    CAGAGAGAAT GTATTTTCCA GTTCACTGGC AATTTTGCGT CATTTTGTTG GCAACATCGA
    CCTGTTCAAA TAATTACATC TAATAATTGT AGAGACTCCT TACTGTGCAC CATTTGCTTG
    GAATTTATAG AGCCTATTCC AACTTACAGC GTGTTACGAA GTCCTTGTTG TAAGAATGCT
    TGGTTTCATA GAGACTGTTT GCAGGTGCAA GCAATAAATG CAGGAATGTT TTTCTTTAGG
    TGCACTATAT GCAATAATAG TGATATATTT CAGAAAGAGA TGTTGAGAAT GGGAATTCAT
    ATTCCTGAAA AAGATGCATC TTGGGAATTA GAGGAAAATG CTTATCAAGA GCTTCTGCAG
    CACTATGAGC ATTGTGATGT CCGAAGATGT CGTTGCAAAG AAGGGCGAGA CTATAATGTG
    CCTGATAGCA AATGGGAAAT AAAGCGCTGT CAGTGTTGTG GTTCTAGCGG AACACATTTA
    GCCTGTTCCT CACTACAGTC ATGGGAACAA AATTGGGAAT GTTTGGAATG TAGAAGTATT
    CTCTACAGTG CAGGCGAATT CCAGAAAGCC AAAAAACATG CATTACCCAA CACTAATGTG
    GGAATAAATG ATTGTTTGTT GGAGGAGTCT TCACCTAAAT TACCCAGACA GTCACCTGGA
    GACCAGTGTA AAGATCTGCT GAGGCAAGGC AGCAAATTTA GAAGAGATAT CTCGACTCTA
    ATTATAGAGT TAGCATTCCA AGTTAAGAAA AGGCCTAAAA AAGTATTTAT CAACAAAGCC
    AATTTCTGGA ATAGTGCCTT AGATGGATTC AGAAATCAAA ACTTTAATCC TTCATATGCA
    ATTGAAATAG TGTATGTTAA TGAAAATGAT CATTTTGGAA GAGAGCAGCC TGGATCAAAG
    CAAGAATTCC TAAGTCTCTT AATGCAGCAA CTTGAGAACT CACCATTATT TGAAGGGTCC
    TTATCAAAGA ACTTGTCTTT AAATTCTCAA GCTCTGAAAG AGAACCTTTA CTATGAAGCT
    GGCAAAATGG TTGCCATTTC TTTGGTTCAT GGTGGTCCTT CACCTGGTTT CTTTTCTCAG
    ACCTTATTTA ACTGCCTTGC TTATGGACCA GAAAATACCC AACCAATTTT AGACGATGTT
    TCAGACTTTG ATGTGGCACA GATTATGATC AGGGTCAATA CTGCAGCAAC TGTGGCTGAC
    TTAAACTCTT TAGTACATGA CTATTATAAG TACTTAGAGC TTATTGGATG TCTAAGACTT
    ATAACATCGA TAAGTGATAA ATATATGTTG GCAAAAGACA TACTTGTTTA TCATGTAATT
    AAAAGAGTCC AAGCACCTTT TGAGAGTTTT AAACAGGGTC TGAAAACTCT TGGTGTTTTG
    GAGAAAATTC AGACTTACCC AGAAGCATTT TTTCACATCT TCTGTCATAA ACCTGAGAAT
    CTTTCTGCAA AAATACTTAG TGATCTTTTT ACAGTACACA CATTGTCTGA TGTACAGACT
    TTGGGGTTTT GGAATAGTTA CTTACAGGCT GTTGAGGATG GTAAATCTAC AGTAACAATG
    GAAGACATTC TTATTTTTGC TACCGGTTGC AAGTCCATTC CACCTGCTGG ATTTAAACCC
    AATCCTTCAA TTGAGTGCCT TCATATGGAT TTTCCTGTAG GAAACAAGTG TGATAACTGT
    TTAGCTCTTC CTATCACCAG TACATATAAA GAATTTCAAG AAAGTATGGA CTTTGCCATA
    AAAAACACTC TAAGATTACA AAAAGAAGAA AATTCTCACT TCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56995
    Clone ID Related Accession (Same CDS sequence) XM_025271539.1 , XM_025271541.1 , XM_025271540.1
    Accession Version XM_025271539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127324.1
    CDS277..2388
    Translation

    Target ORF information:

    RefSeq Version XM_025271539.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56995
    Clone ID Related Accession (Same CDS sequence) XM_025271539.1 , XM_025271541.1 , XM_025271540.1
    Accession Version XM_025271541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127326.1
    CDS917..3028
    Translation

    Target ORF information:

    RefSeq Version XM_025271541.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56995
    Clone ID Related Accession (Same CDS sequence) XM_025271539.1 , XM_025271541.1 , XM_025271540.1
    Accession Version XM_025271540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127325.1
    CDS439..2550
    Translation

    Target ORF information:

    RefSeq Version XM_025271540.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAATGAAA GTAAACCTGG AGGCTCACAG AATTCTACTT GTGTTTTCTG TCGAAAAAAT 
    GATGACTGTC CTATTAAATA TGGAGAAAAG AAAACTAATG AGAAATGGAA TCTCACTGTA
    CATTACTATT GTTTGTTGAT GTCAAGTGGA ATTTGGCAGA GGGGTAAAGA AGAAGAAGGA
    GTTTATGGTT TTCTAATAGA AGATATCAGG AAAGAAGTGA CTAGGGCTTC TAAACTGAAA
    TGCTCTGTTT GCAAGAAAAT GGGTGCTTCA ATTGGGTGTG TCGCACCCCG ATGTAAACGA
    AGTTATCATT TCCCATGTGG ACTTCAGAGA GAATGTATTT TCCAGTTCAC TGGCAATTTT
    GCGTCATTTT GTTGGCAACA TCGACCTGTT CAAATAATTA CATCTAATAA TTGTAGAGAC
    TCCTTACTGT GCACCATTTG CTTGGAATTT ATAGAGCCTA TTCCAACTTA CAGCGTGTTA
    CGAAGTCCTT GTTGTAAGAA TGCTTGGTTT CATAGAGACT GTTTGCAGGT GCAAGCAATA
    AATGCAGGAA TGTTTTTCTT TAGGTGCACT ATATGCAATA ATAGTGATAT ATTTCAGAAA
    GAGATGTTGA GAATGGGAAT TCATATTCCT GAAAAAGATG CATCTTGGGA ATTAGAGGAA
    AATGCTTATC AAGAGCTTCT GCAGCACTAT GAGCATTGTG ATGTCCGAAG ATGTCGTTGC
    AAAGAAGGGC GAGACTATAA TGTGCCTGAT AGCAAATGGG AAATAAAGCG CTGTCAGTGT
    TGTGGTTCTA GCGGAACACA TTTAGCCTGT TCCTCACTAC AGTCATGGGA ACAAAATTGG
    GAATGTTTGG AATGTAGAAG TATTCTCTAC AGTGCAGGCG AATTCCAGAA AGCCAAAAAA
    CATGCATTAC CCAACACTAA TGTGGGAATA AATGATTGTT TGTTGGAGGA GTCTTCACCT
    AAATTACCCA GACAGTCACC TGGAGACCAG TGTAAAGATC TGCTGAGGCA AGGCAGCAAA
    TTTAGAAGAG ATATCTCGAC TCTAATTATA GAGTTAGCAT TCCAAGTTAA GAAAAGGCCT
    AAAAAAGTAT TTATCAACAA AGCCAATTTC TGGAATAGTG CCTTAGATGG ATTCAGAAAT
    CAAAACTTTA ATCCTTCATA TGCAATTGAA ATAGTGTATG TTAATGAAAA TGATCATTTT
    GGAAGAGAGC AGCCTGGATC AAAGCAAGAA TTCCTAAGTC TCTTAATGCA GCAACTTGAG
    AACTCACCAT TATTTGAAGG GTCCTTATCA AAGAACTTGT CTTTAAATTC TCAAGCTCTG
    AAAGAGAACC TTTACTATGA AGCTGGCAAA ATGGTTGCCA TTTCTTTGGT TCATGGTGGT
    CCTTCACCTG GTTTCTTTTC TCAGACCTTA TTTAACTGCC TTGCTTATGG ACCAGAAAAT
    ACCCAACCAA TTTTAGACGA TGTTTCAGAC TTTGATGTGG CACAGATTAT GATCAGGGTC
    AATACTGCAG CAACTGTGGC TGACTTAAAC TCTTTAGTAC ATGACTATTA TAAGTACTTA
    GAGCTTATTG GATGTCTAAG ACTTATAACA TCGATAAGTG ATAAATATAT GTTGGCAAAA
    GACATACTTG TTTATCATGT AATTAAAAGA GTCCAAGCAC CTTTTGAGAG TTTTAAACAG
    GGTCTGAAAA CTCTTGGTGT TTTGGAGAAA ATTCAGACTT ACCCAGAAGC ATTTTTTCAC
    ATCTTCTGTC ATAAACCTGA GAATCTTTCT GCAAAAATAC TTAGTGATCT TTTTACAGTA
    CACACATTGT CTGATGTACA GACTTTGGGG TTTTGGAATA GTTACTTACA GGCTGTTGAG
    GATGGTAAAT CTACAGTAAC AATGGAAGAC ATTCTTATTT TTGCTACCGG TTGCAAGTCC
    ATTCCACCTG CTGGATTTAA ACCCAATCCT TCAATTGAGT GCCTTCATAT GGATTTTCCT
    GTAGGAAACA AGTGTGATAA CTGTTTAGCT CTTCCTATCA CCAGTACATA TAAAGAATTT
    CAAGAAAGTA TGGACTTTGC CATAAAAAAC ACTCTAAGAT TACAAAAAGA AGAAAATTCT
    CACTTCATTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56996
    Clone ID Related Accession (Same CDS sequence) XM_025271542.1
    Accession Version XM_025271542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTATTGATGT CAAGTGGAAT TTGGCAGAGG 
    GGTAAAGAAG AAGAAGGAGT TTATGGTTTT CTAATAGAAG ATATCAGGAA AGAAGTGACT
    AGGGCTTCTA AACTGAAATG CTCTGTTTGC AAGAAAATGG GTGCTTCAAT TGGGTGTGTC
    GCACCCCGAT GTAAACGAAG TTATCATTTC CCATGTGGAC TTCAGAGAGA ATGTATTTTC
    CAGTTCACTG GCAATTTTGC GTCATTTTGT TGGCAACATC GACCTGTTCA AATAATTACA
    TCTAATAATT GTAGAGACTC CTTACTGTGC ACCATTTGCT TGGAATTTAT AGAGCCTATT
    CCAACTTACA GCGTGTTACG AAGTCCTTGT TGTAAGAATG CTTGGTTTCA TAGAGACTGT
    TTGCAGGTGC AAGCAATAAA TGCAGGAATG TTTTTCTTTA GGTGCACTAT ATGCAATAAT
    AGTGATATAT TTCAGAAAGA GATGTTGAGA ATGGGAATTC ATATTCCTGA AAAAGATGCA
    TCTTGGGAAT TAGAGGAAAA TGCTTATCAA GAGCTTCTGC AGCACTATGA GCATTGTGAT
    GTCCGAAGAT GTCGTTGCAA AGAAGGGCGA GACTATAATG TGCCTGATAG CAAATGGGAA
    ATAAAGCGCT GTCAGTGTTG TGGTTCTAGC GGAACACATT TAGCCTGTTC CTCACTACAG
    TCATGGGAAC AAAATTGGGA ATGTTTGGAA TGTAGAAGTA TTCTCTACAG TGCAGGCGAA
    TTCCAGAAAG CCAAAAAACA TGCATTACCC AACACTAATG TGGGAATAAA TGATTGTTTG
    TTGGAGGAGT CTTCACCTAA ATTACCCAGA CAGTCACCTG GAGACCAGTG TAAAGATCTG
    CTGAGGCAAG GCAGCAAATT TAGAAGAGAT ATCTCGACTC TAATTATAGA GTTAGCATTC
    CAAGTTAAGA AAAGGCCTAA AAAAGTATTT ATCAACAAAG CCAATTTCTG GAATAGTGCC
    TTAGATGGAT TCAGAAATCA AAACTTTAAT CCTTCATATG CAATTGAAAT AGTGTATGTT
    AATGAAAATG ATCATTTTGG AAGAGAGCAG CCTGGATCAA AGCAAGAATT CCTAAGTCTC
    TTAATGCAGC AACTTGAGAA CTCACCATTA TTTGAAGGGT CCTTATCAAA GAACTTGTCT
    TTAAATTCTC AAGCTCTGAA AGAGAACCTT TACTATGAAG CTGGCAAAAT GGTTGCCATT
    TCTTTGGTTC ATGGTGGTCC TTCACCTGGT TTCTTTTCTC AGACCTTATT TAACTGCCTT
    GCTTATGGAC CAGAAAATAC CCAACCAATT TTAGACGATG TTTCAGACTT TGATGTGGCA
    CAGATTATGA TCAGGGTCAA TACTGCAGCA ACTGTGGCTG ACTTAAACTC TTTAGTACAT
    GACTATTATA AGTACTTAGA GCTTATTGGA TGTCTAAGAC TTATAACATC GATAAGTGAT
    AAATATATGT TGGCAAAAGA CATACTTGTT TATCATGTAA TTAAAAGAGT CCAAGCACCT
    TTTGAGAGTT TTAAACAGGG TCTGAAAACT CTTGGTGTTT TGGAGAAAAT TCAGACTTAC
    CCAGAAGCAT TTTTTCACAT CTTCTGTCAT AAACCTGAGA ATCTTTCTGC AAAAATACTT
    AGTGATCTTT TTACAGTACA CACATTGTCT GATGTACAGA CTTTGGGGTT TTGGAATAGT
    TACTTACAGG CTGTTGAGGA TGGTAAATCT ACAGTAACAA TGGAAGACAT TCTTATTTTT
    GCTACCGGTT GCAAGTCCAT TCCACCTGCT GGATTTAAAC CCAATCCTTC AATTGAGTGC
    CTTCATATGG ATTTTCCTGT AGGAAACAAG TGTGATAACT GTTTAGCTCT TCCTATCACC
    AGTACATATA AAGAATTTCA AGAAAGTATG GACTTTGCCA TAAAAAACAC TCTAAGATTA
    CAAAAAGAAG AAAATTCTCA CTTCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127327.1
    CDS143..2152
    Translation

    Target ORF information:

    RefSeq Version XM_025271542.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAAGTAA ACCTGGAGGC TCACAGAATT CTATTGATGT CAAGTGGAAT TTGGCAGAGG 
    GGTAAAGAAG AAGAAGGAGT TTATGGTTTT CTAATAGAAG ATATCAGGAA AGAAGTGACT
    AGGGCTTCTA AACTGAAATG CTCTGTTTGC AAGAAAATGG GTGCTTCAAT TGGGTGTGTC
    GCACCCCGAT GTAAACGAAG TTATCATTTC CCATGTGGAC TTCAGAGAGA ATGTATTTTC
    CAGTTCACTG GCAATTTTGC GTCATTTTGT TGGCAACATC GACCTGTTCA AATAATTACA
    TCTAATAATT GTAGAGACTC CTTACTGTGC ACCATTTGCT TGGAATTTAT AGAGCCTATT
    CCAACTTACA GCGTGTTACG AAGTCCTTGT TGTAAGAATG CTTGGTTTCA TAGAGACTGT
    TTGCAGGTGC AAGCAATAAA TGCAGGAATG TTTTTCTTTA GGTGCACTAT ATGCAATAAT
    AGTGATATAT TTCAGAAAGA GATGTTGAGA ATGGGAATTC ATATTCCTGA AAAAGATGCA
    TCTTGGGAAT TAGAGGAAAA TGCTTATCAA GAGCTTCTGC AGCACTATGA GCATTGTGAT
    GTCCGAAGAT GTCGTTGCAA AGAAGGGCGA GACTATAATG TGCCTGATAG CAAATGGGAA
    ATAAAGCGCT GTCAGTGTTG TGGTTCTAGC GGAACACATT TAGCCTGTTC CTCACTACAG
    TCATGGGAAC AAAATTGGGA ATGTTTGGAA TGTAGAAGTA TTCTCTACAG TGCAGGCGAA
    TTCCAGAAAG CCAAAAAACA TGCATTACCC AACACTAATG TGGGAATAAA TGATTGTTTG
    TTGGAGGAGT CTTCACCTAA ATTACCCAGA CAGTCACCTG GAGACCAGTG TAAAGATCTG
    CTGAGGCAAG GCAGCAAATT TAGAAGAGAT ATCTCGACTC TAATTATAGA GTTAGCATTC
    CAAGTTAAGA AAAGGCCTAA AAAAGTATTT ATCAACAAAG CCAATTTCTG GAATAGTGCC
    TTAGATGGAT TCAGAAATCA AAACTTTAAT CCTTCATATG CAATTGAAAT AGTGTATGTT
    AATGAAAATG ATCATTTTGG AAGAGAGCAG CCTGGATCAA AGCAAGAATT CCTAAGTCTC
    TTAATGCAGC AACTTGAGAA CTCACCATTA TTTGAAGGGT CCTTATCAAA GAACTTGTCT
    TTAAATTCTC AAGCTCTGAA AGAGAACCTT TACTATGAAG CTGGCAAAAT GGTTGCCATT
    TCTTTGGTTC ATGGTGGTCC TTCACCTGGT TTCTTTTCTC AGACCTTATT TAACTGCCTT
    GCTTATGGAC CAGAAAATAC CCAACCAATT TTAGACGATG TTTCAGACTT TGATGTGGCA
    CAGATTATGA TCAGGGTCAA TACTGCAGCA ACTGTGGCTG ACTTAAACTC TTTAGTACAT
    GACTATTATA AGTACTTAGA GCTTATTGGA TGTCTAAGAC TTATAACATC GATAAGTGAT
    AAATATATGT TGGCAAAAGA CATACTTGTT TATCATGTAA TTAAAAGAGT CCAAGCACCT
    TTTGAGAGTT TTAAACAGGG TCTGAAAACT CTTGGTGTTT TGGAGAAAAT TCAGACTTAC
    CCAGAAGCAT TTTTTCACAT CTTCTGTCAT AAACCTGAGA ATCTTTCTGC AAAAATACTT
    AGTGATCTTT TTACAGTACA CACATTGTCT GATGTACAGA CTTTGGGGTT TTGGAATAGT
    TACTTACAGG CTGTTGAGGA TGGTAAATCT ACAGTAACAA TGGAAGACAT TCTTATTTTT
    GCTACCGGTT GCAAGTCCAT TCCACCTGCT GGATTTAAAC CCAATCCTTC AATTGAGTGC
    CTTCATATGG ATTTTCCTGT AGGAAACAAG TGTGATAACT GTTTAGCTCT TCCTATCACC
    AGTACATATA AAGAATTTCA AGAAAGTATG GACTTTGCCA TAAAAAACAC TCTAAGATTA
    CAAAAAGAAG AAAATTCTCA CTTCATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu56997
    Clone ID Related Accession (Same CDS sequence) XM_025271543.1
    Accession Version XM_025271543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product G2/M phase-specific E3 ubiquitin-protein ligase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGATGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025127328.1
    CDS126..1877
    Translation

    Target ORF information:

    RefSeq Version XM_025271543.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis G2/M-phase specific E3 ubiquitin protein ligase (G2E3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025271543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGTGTG GCGCAGCGCG GGAAGGGAAA AGTGACACGG CCTCTCTGGA ACTTCTGCCC 
    AGTGCGCCTC GTAAAATGAA TGAAAGTAAA CCTGGAGGCT CACAGAATTC TACTTGTGTT
    TTCTGTCGAA AAAATGATGA CTGTCCTATT AAATATGGAG AAAAGAAAAC TAATGAGAAA
    TGGAATCTCA CTGTACATTA CTATTGTTTG TTGATGTCAA GTGGAATTTG GCAGAGGGGT
    AAAGAAGAAG AAGGAGTTTA TGGTTTTCTA ATAGAAGATA TCAGGAAAGA AGTGACTAGG
    GCTTCTAAAC TGAAATGCTC TGTTTGCAAG AAAATGGGTG CTTCAATTGG GTGTGTCGCA
    CCCCGATGTA AACGAAGTTA TCATTTCCCA TGTGGACTTC AGAGAGAATG TATTTTCCAG
    TTCACTGGCA ATTTTGCGTC ATTTTGTTGG CAACATCGAC CTGTTCAAAT AATTACATCT
    AATAATTGTA GAGACTCCTT ACTGTGCACC ATTTGCTTGG AATTTATAGA GCCTATTCCA
    ACTTACAGCG TGTTACGAAG TCCTTGTTGT AAGAATGCTT GGTTTCATAG AGACTGTTTG
    CAGGTGCAAG CAATAAATGC AGGAATGTTT TTCTTTAGGT GCACTATATG CAATAATAGT
    GATATATTTC AGAAAGAGAT GTTGAGAATG GGAATTCATA TTCCTGAAAA AGATGCATCT
    TGGGAATTAG AGGAAAATGC TTATCAAGAG CTTCTGCAGC ACTATGAGCA TTGTGATGTC
    CGAAGATGTC GTTGCAAAGA AGGGCGAGAC TATAATGTGC CTGATAGCAA ATGGGAAATA
    AAGCGCTGTC AGTGTTGTGG TTCTAGCGGA ACACATTTAG CCTGTTCCTC ACTACAGTCA
    TGGGAACAAA ATTGGGAATG TTTGGAATGT AGAAGTATTC TCTACAGTGC AGGCGAATTC
    CAGAAAGCCA AAAAACATGC ATTACCCAAC ACTAATGTGG GAATAAATGA TTGTTTGTTG
    GAGGAGTCTT CACCTAAATT ACCCAGACAG TCACCTGGAG ACCAGTGTAA AGATCTGCTG
    AGGCAAGGCA GCAAATTTAG AAGAGATATC TCGACTCTAA TTATAGAGTT AGCATTCCAA
    GTTAAGAAAA GGCCTAAAAA AGTATTTATC AACAAAGCCA ATTTCTGGAA TAGTGCCTTA
    GATGGATTCA GAAATCAAAA CTTTAATCCT TCATATGCAA TTGAAATAGT GTATGTTAAT
    GAAAATGATC ATTTTGGAAG AGAGCAGCCT GGATCAAAGC AAGAATTCCT AAGTCTCTTA
    ATGCAGCAAC TTGAGAACTC ACCATTATTT GAAGGGTCCT TATCAAAGAA CTTGTCTTTA
    AATTCTCAAG CTCTGAAAGA GAACCTTTAC TATGAAGCTG GCAAAATGGT TGCCATTTCT
    TTGGTTCATG GTGGTCCTTC ACCTGGTTTC TTTTCTCAGA CCTTATTTAA CTGCCTTGCT
    TATGGACCAG AAAATACCCA ACCAATTTTA GACGATGTTT CAGACTTTGA TGTGGCACAG
    ATTATGATCA GGGTCAATAC TGCAGCAACT GTGGCTGACT TAAACTCTTT AGTACATGAC
    TATTATAAGT ACTTAGAGCT TATTGGATGT CTAAGACTTA TAACATCGAT AAGTGATAAA
    TATATGTTGG CAAAAGACAT ACTTGTTTAT CATGTAATTA AAAGAGTCCA AGCACCTTTT
    GAGAGATGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.