LRRK1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRK1
Entrez Gene ID	102394512
Full Name	leucine rich repeat kinase 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025271634.1	XP_025127419.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
XM_025271631.1	XP_025127416.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_025271629.1	XP_025127414.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_025271632.1	XP_025127417.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_025271633.1	XP_025127418.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_006070641.1	XP_006070703.1	leucine-rich repeat serine/threonine-protein kinase 1
XM_025271635.1	XP_025127420.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LRRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu57061	XM_025271634.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu57062	XM_025271635.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu25198	XM_006070641.1 Latest version!	Bubalus bubalis leucine-rich repeat kinase 1 (LRRK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBu57060	XM_025271629.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu57060	XM_025271632.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu57060	XM_025271631.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBu57060	XM_025271633.1 Latest version!	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRK1 ( XM_025271634.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127419.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu57061
Clone ID Related Accession (Same CDS sequence)	XM_025271634.1
Accession Version	XM_025271634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA AACTCTCCAT GCCAGGCAGT GAGCCCATGG TGCAGAGCCC CAGGACGGAA 
GGCATTCATG CTGCGTACAG GCGAGGAGAC ATCAGCAGAG CCCAGCATCT GCTCCGAGAA 
GCTTGTGACG AGAGCACATC CCAGCTGGAA AAGGGCCAGC TCCTGAGTAT CTCTGCAGCC 
CATGGTGACC TGGAGACCGT CCGGTATCTT CTCACTGAGA GGCTGGTGGA GCTACCGACG 
GAGCCCACTG ACGACAACCC TGCCGTGGTT GCAGCACAGT TTGGGCACAC TGATGTCGTC 
CAGGAGCTAC TCGAGTCCTT GCCAGGTCCC TGCAGCCCCC AGCGGCTCTT GAATTGGATG 
CTGGCCTTGG CCTGCCAGCA GGGGCACCTG GCAGTGGTGA AGCTCCTGGT CCTGACCTAC 
GGGGCCGACC CGGAGAGCTA CGCAGTCAGG AAGAACGAGT TCCCTGTCAT CGTGCGCCTG 
CCGCTGTACG CGGCCATCAA GTCAGGGAAT GAAGATATTG CCATCTTCCT GATTCGGCAT 
GGAGCCTTTT TCTGCTCCTA CATTTTACTG GACAGTCCTG ACCCCAGCAA ACATCTCCTC 
AGGAAGTATT TTGTTGAAGC AAGCGCCTTG CCCAGCAGTT GTCCGGGAAA AATGGCTCTT 
CGTGTGAAGT GGTCCCACCT CAAGCTGCCC TGGGTGGATC TGGACTGGCT CATCGACATC 
TCCTGCCAGA TCACAGAGCT GGATCTTTCT GCCAACTGCC TGGCGTCCCT CCCCTCCGTC 
ATCCCCTGGG GCCTCATCAA CCTCCGGAAG CTGAACCTCT CAGACAACCA CCTGGGGGAG 
CTGCCCAGCG TGCAGTCATC AGATGAAATC ATCTGTTCCA GGCTACTTGA AATTGACATT 
TCCAGCAACA AGTTGTCCCA CCTCCCACCA GGATTCCTGC ATCTCTCAAA ACTTCAAAAA 
CTGACGGCTT CAAAAAACTA TTTGGAAAAA TTGTTTGAAG AAGAGAGTGC CACGAACTGG 
ATTGGTTTAC GGAAGCTACA GGAGCTCGAT GTTTCTGATA ACAAACTGAC AGAGCTCCCT 
GCACTTTTCC TCCATTCTTT CAAATCGCTC AGTTGTCTCA ACGTCTCCAG AAATAACCTG 
AAGGCGTTTC CAGATGCCTG GGCTTGCCCT TTGAAATGTT GCAAAGCATC CAAAAATGCC 
CTGGAGTCTC TACCAGACAA AATGGCTGTG TTTTGGAAGA ACCACCTGAA GGATGTGGAC 
TTCTCAGAAA ACGCACTGAA AGAAGTTCCC CTGGGACTTT TCCAACTTGA TGCCCTCATG 
TTCTTGAAGT TACAGGGAAA CCAGCTGGTG GCACTCCCAC CTCAAGAGAA GTGGACCTGC 
AGACAGCTCA AAACCCTGGA TCTCTCCAGA AACCACTTTG GCAAAAACGA AGACGTCCTT 
AAAACAAAGC GCATTTCCTT TTTCACCACC AGAGGCCGCC AGCGTTCGGG GACGGAGGCA 
GCGTGTGTAC TCGAATTTCC CGCCTTCCTA AGTGAGTCTT TGGAAGTCCT CTGTCTGAAC 
GACAACCACC TGGATGCAGT CCCACCCTCA GTTTGCCTAC TGAAGAGCTT ATCAGAGCTC 
TACTTGGGGA ACAATCCTAG CCTCCGAGAG CTCCCTCCTG AGCTGGGGCA GCTGGGGAAC 
CTCTGGCAGT TGGACATTGA AGACCTGAAT ATCGTCAATG TGCCTGCAGA GATCAGAAAA 
GAAGGCCCCA AAGCAATGCT GTCTTACCTG CGTGCTCAGC TGCGGAAAGC AGAGAAGTGC 
AAGCAGATGA AGATGATCAT CGTGGGTCCG CCGCGTCAGG GCAAGTCCAC CCTCCTGGAG 
ATCTTACAGA CGGGGAGGGC CCCCCAGGTG GTACACAGCG AGGCCACCAT CAGGACCACC 
AAGTGGGAGC TGCAGAGGCC GGCTGGCTCG AGAGCCAAGG TTGAGTCTGT GGAGTTCACC 
GTCTGGGACA TCGGCGGCCC CGCCAGCATG GCCACGGTCA ACCAGTGCTT CTTCACGGAC 
AAGGCCCTGT ATGTGGTGGT CTGGAACCTG GCTCTGGGGG AGGAGGCGGT GGCCAGCCTT 
CAGTTCTGGC TGCTCAACAT CGAGGCCAAG GCCCCAAACG CTGTGGTGCT GGTGGTGGGA 
ACACATCTGG ACTTAATTGA AACCAAATTC CGTGTGGAGA GGATCGCCAC TCTACGCGCC 
TACGTGCTGG CCCTCTGCCG CTCGCCCTCT GGGTCCAGGG CCACCGGCTT CCCGGACATC 
ACGTGTAAAC ACCTGCACGA ACTCTCCTGT AAGAGCTTGG AAGGTCAGGA AGGGCTGCGG 
CAGCTCATTT TCCAGGTCAC GTGCAACATG AAGGACGTGG GCAGCACCAT CGGCTGTCAG 
CGACTTGCAG GGAGGCTGAT CCCCAGGAGC TACTTGAGCC TCCAGGAGGC TGTGCTGGCG 
GAGCAGCAGC GCCGCAGCCT GAGCGACGAC GTGCAGTACC TGACGGACCG GCAGCTGGAG 
CAGCTGGTAG ACCAGATGCC CTGCAGCGAC ATCCAGGACT ACGAGGATCT GCAGTCTGCC 
ATCAGCTTCC TCATTGAAAC GGGCTCCCTG CTGCACTTCC CGGACACCAG CCACGGCCTG 
AGGAACCTCT ACTTCCTAGA CCCCATCTGG CTGTCCGAGT GTCTGCAGAG GATCTTCAAC 
ATCAAGGGCT CTCGGTCAGT GGCCAAGAAC GGGGTGATCA GGGCCGAGGA CCTTCGGCTG 
CTGCTGGTTG GCACGGGCTT CACGAAGCAG ACAGAAGAGC AGTACTTCCA GTTCCTGGCC 
AAATTTGAGA TCGCCCTACC CGTTGCCAAT GACAGCTATC TCCTGCCACA TCTCCTTCCA 
TCCAAACCCG GCCTGGACAC CCACGGCATG CGGCACCCCA CAGCCAACAC CATTCAGAGA 
GTGTTTAAGA TGAGCTTCGT CCCGGTTGGC TTCTGGCAGA GGTTTATAGC GCGGATGCTG 
ATCAGCTTGG CCGAGATGGA CCTCCAGCTG TTTGAAAACA GGAAGAATAC TAAGAGCAGG 
CACAGGAAAG TCACCATTTA TAGTTTTACA GGAAACCAGA GAAATCGTTG CAGCACATTC 
AGAGTGAAAA GGAACCAGAC CATCTATTGG CAGGAGGGGC TCCTGGTCAC TTTTGATGGG 
GGCTACCTCA GTGTAGAATC CTCGGATGTG AACTGGAAAA AGAAAAAAAG TGGAGGAATA 
AAAATCATGT GCCAATCAGA AATAAGGGAC TTCTCGGCAA TGGCTTTTAT CACAGACCAT 
GTCAATTCCT TGATCGATCA GTGGTTTCCC GCCCTGACGG CCACGGAAAG TGACGGGACC 
CCACTGATGG AGCAGTATGT GCCCTGCCCT GTCTGCGAGG CCTCTTGGGC TCAGCACAGC 
AACCCCAGCG AGAGGTCAGA GGATGTGCAG TACTTCGACA TGGAGGACTG CGTGCTGACA 
GCCATTGAGC GGGACTTCAT CTCCTGCCCC AGGCACCCTG ACCTCCCGGT GCCTCTCCAG 
GAACTGGTCC CCGAGCTGTT CATGACCGAC TTCCCAGCCA GGCTCTTCTT GGAGAACAGC 
AAGCTGGAGC ACAGCGAGGA TGAGAGTGCT GTCCTGGGCC AAGGTGGGAG TGGCACCGTC 
ATCTACCGGG CCAGGTACCA AGGCCAGCCT GTGGCTGTGA AGCGATTCCA GATCAAGAAA 
TTCAAGAACT GTGCAAATGC CCCTGCAGAT ACCATGCTGA GGCACCTGCG GGCCACTGAC 
GCCATGAGGA ACTTCTCGGA GTTCCGGCAG GAGGCCAGCA TGCTGCACGC CCTGCAGCAC 
CCCTGCATCG TGTCCCTCAT CGGCATCAGC ATCCACCCGC TCTGCTTCGC GCTGGAGCTT 
GCCCCGCTGG GCAGCCTCAA CACCGTGCTG TCCGAGAACG CCAGAGATTC CTCCTTTATG 
CCCTTGGGAC ACATGCTCAC CCAAAAAATA GCCTACCAGA TAGCCTCTGG CCTGGCCTAC 
CTGCACAAGA AAAACATCAT CTTCTGTGAC CTGAAGTCCG ACAACATCCT GGTCTGGTCT 
CTGGACATCA AGGAGCACAT CAACATCAAG CTGTCCGACT ACGGCATCTC CCGGCAGTCA 
TTCCACGAGG GTGCCCTCGG CGTGGAGGGT ACTCCCGGCT ACCAGGCCCC GGAGATCCGG 
CCTCGCATCG TGTATGACGA GAAGGTAGAC ATGTTCTCCT ATGGGATGGT GCTCTACGAG 
TTGCTGTCAG GACAGCGGCC CGCACTGGGC CAGCACCAGC TTCAGATCTC CAAGAAGCTG 
TCCAAGGGCA TCCGCCCAGT TCTGGGTCAG CCGGAGGAAG TGCAGTTCCA CCGACTGCAG 
GCGCTTATGA TGGAGTGCTG GGATACGAAG CCCGAGAAGC GACCACTGGC CCTGGCGGTG 
GTGAGCCAAA TGAAGGACCC GACCTTTGCG ACCTTCATGT ACCAACTGCC CTGCGGGAAG 
CAGACATCCT TCTTCTCATC CCAGGGCCAG GAGTACATGG TGGTGTTCTG GGAAGGCAAG 
GAGGAGTCCA GGAACTACAC GGTGGTGAAC ACGGAGAAGG GCCTTCTGGA AGTGCAGCAC 
ATGAGCTGTG CGGGAATGAA GCTGAGCTGC CAACTCAAGA TCCAGAACTC GCTGTGGACG 
GCCACCGAGG ACCAGAAGAT CTACATCTAC AGCCTCAAGG GCATGTGTCC ACTGAACACA 
CCCCAGCGGG CTTTGGACAC CCCGGCCATC GTCACCTGCT TCTTGGCGGT ACCTGTTATA 
AAAAAGAACT CCCACCTGGT GCTGGCAGGC CTAGCGGACG GCCTGGTGGC GGTGTTCCCG 
GCAGCGTGGG GCGCCCCGGA CTACGGCTGC TCCTACCTGT GCTCGCACAC AGCCAACAGG 
GCCAAGTTCA GCATCCCCGA CGAGGACGCC CGGCAGAACC CCTACCCCGT GACGGCCATG 
GAGGTGGTCA ACTGCGGGTC CGAGGTCTGG TACAGCAACG GGCCAGGCCT GCTGGTCATT 
GACTGTGCGG CCCTGGAGAT CCGCCGGCGG CTGGAGCCCT ACGCAGCGCC CTCGGTGGTC 
ACATCTGTGG TGTGTGGCTC GGAGTGCCGT GGCGAGGAGG TGGTGTGGTG CCTGGACGAT 
AGGGCCAACT GCCTGGTGCT ATACCACTCG GCGACCTACC AGCTGTGCGC CCGCTACTTC 
TGCGGGGACC CCAGCCCCCT AGGGGACGTG TTTCCCGTGT GCCCGATGGG CCCACCACCC 
CTGGACAGCC CCACAGCCAG CGCGAGGGAG CCCGCGAGGG GCTCCATCGC CGACGTGAGC 
TTCATGTTCA GCGAGGAGCT GGGCACGCAG ATCCTCACCC ACCAGGACTC GCTGACCGAC 
TACTGTTCCC TATCCTCCTG CTCCGCGGCC TCGCCACACC CGGCACCCAT GGCCCTGTCC 
AGCCTGCCCT GCTCCCCGGC CAGCTCTGCC AGCTTGCCCT TCTCCTCTGA CTGCGAGGAC 
ACCAGCCAGC TGCCCAAGCC CACTGTGGGC TCCGACAGGT CGGTGCATGA CCTGAGCCCA 
GTGGACGGCG AGGCGTTCAG CCAGCACCTG CAGGCTGTGA AGATCCTTGC CGTGAAAGAC 
GTCATCTGGG TCCCCAGGCG CGGTGGAGAT ATCATCGTCA TTAGCCTGGA GAAGGAGGCA 
GGAACTCAGC GGGGCCACGT GATTGCTGTC TTGAAAGCCC GAGAGCTGAC TCCGCACGGG 
GTTCTCACGG AGGCTGCCGT GGTGGCAAAG GACACAGTTG TGTGCGGCTT TGAGAATGAA 
AATCTGGAGT GGTGCCTGGC TGTCTGGAGG GGCTGGGGCA TGCAGGAGTT TGACATTTTC 
TACCAGTCCT ACGAGGAGCT GGGCCGGCTG GAGGCCTGCG TGCGGAAGAG GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127419.1
CDS	1920..7856
Translation	MGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271634.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271634.1

ORF Insert Sequence:

ATGGGCAGCA AACTCTCCAT GCCAGGCAGT GAGCCCATGG TGCAGAGCCC CAGGACGGAA 
GGCATTCATG CTGCGTACAG GCGAGGAGAC ATCAGCAGAG CCCAGCATCT GCTCCGAGAA 
GCTTGTGACG AGAGCACATC CCAGCTGGAA AAGGGCCAGC TCCTGAGTAT CTCTGCAGCC 
CATGGTGACC TGGAGACCGT CCGGTATCTT CTCACTGAGA GGCTGGTGGA GCTACCGACG 
GAGCCCACTG ACGACAACCC TGCCGTGGTT GCAGCACAGT TTGGGCACAC TGATGTCGTC 
CAGGAGCTAC TCGAGTCCTT GCCAGGTCCC TGCAGCCCCC AGCGGCTCTT GAATTGGATG 
CTGGCCTTGG CCTGCCAGCA GGGGCACCTG GCAGTGGTGA AGCTCCTGGT CCTGACCTAC 
GGGGCCGACC CGGAGAGCTA CGCAGTCAGG AAGAACGAGT TCCCTGTCAT CGTGCGCCTG 
CCGCTGTACG CGGCCATCAA GTCAGGGAAT GAAGATATTG CCATCTTCCT GATTCGGCAT 
GGAGCCTTTT TCTGCTCCTA CATTTTACTG GACAGTCCTG ACCCCAGCAA ACATCTCCTC 
AGGAAGTATT TTGTTGAAGC AAGCGCCTTG CCCAGCAGTT GTCCGGGAAA AATGGCTCTT 
CGTGTGAAGT GGTCCCACCT CAAGCTGCCC TGGGTGGATC TGGACTGGCT CATCGACATC 
TCCTGCCAGA TCACAGAGCT GGATCTTTCT GCCAACTGCC TGGCGTCCCT CCCCTCCGTC 
ATCCCCTGGG GCCTCATCAA CCTCCGGAAG CTGAACCTCT CAGACAACCA CCTGGGGGAG 
CTGCCCAGCG TGCAGTCATC AGATGAAATC ATCTGTTCCA GGCTACTTGA AATTGACATT 
TCCAGCAACA AGTTGTCCCA CCTCCCACCA GGATTCCTGC ATCTCTCAAA ACTTCAAAAA 
CTGACGGCTT CAAAAAACTA TTTGGAAAAA TTGTTTGAAG AAGAGAGTGC CACGAACTGG 
ATTGGTTTAC GGAAGCTACA GGAGCTCGAT GTTTCTGATA ACAAACTGAC AGAGCTCCCT 
GCACTTTTCC TCCATTCTTT CAAATCGCTC AGTTGTCTCA ACGTCTCCAG AAATAACCTG 
AAGGCGTTTC CAGATGCCTG GGCTTGCCCT TTGAAATGTT GCAAAGCATC CAAAAATGCC 
CTGGAGTCTC TACCAGACAA AATGGCTGTG TTTTGGAAGA ACCACCTGAA GGATGTGGAC 
TTCTCAGAAA ACGCACTGAA AGAAGTTCCC CTGGGACTTT TCCAACTTGA TGCCCTCATG 
TTCTTGAAGT TACAGGGAAA CCAGCTGGTG GCACTCCCAC CTCAAGAGAA GTGGACCTGC 
AGACAGCTCA AAACCCTGGA TCTCTCCAGA AACCACTTTG GCAAAAACGA AGACGTCCTT 
AAAACAAAGC GCATTTCCTT TTTCACCACC AGAGGCCGCC AGCGTTCGGG GACGGAGGCA 
GCGTGTGTAC TCGAATTTCC CGCCTTCCTA AGTGAGTCTT TGGAAGTCCT CTGTCTGAAC 
GACAACCACC TGGATGCAGT CCCACCCTCA GTTTGCCTAC TGAAGAGCTT ATCAGAGCTC 
TACTTGGGGA ACAATCCTAG CCTCCGAGAG CTCCCTCCTG AGCTGGGGCA GCTGGGGAAC 
CTCTGGCAGT TGGACATTGA AGACCTGAAT ATCGTCAATG TGCCTGCAGA GATCAGAAAA 
GAAGGCCCCA AAGCAATGCT GTCTTACCTG CGTGCTCAGC TGCGGAAAGC AGAGAAGTGC 
AAGCAGATGA AGATGATCAT CGTGGGTCCG CCGCGTCAGG GCAAGTCCAC CCTCCTGGAG 
ATCTTACAGA CGGGGAGGGC CCCCCAGGTG GTACACAGCG AGGCCACCAT CAGGACCACC 
AAGTGGGAGC TGCAGAGGCC GGCTGGCTCG AGAGCCAAGG TTGAGTCTGT GGAGTTCACC 
GTCTGGGACA TCGGCGGCCC CGCCAGCATG GCCACGGTCA ACCAGTGCTT CTTCACGGAC 
AAGGCCCTGT ATGTGGTGGT CTGGAACCTG GCTCTGGGGG AGGAGGCGGT GGCCAGCCTT 
CAGTTCTGGC TGCTCAACAT CGAGGCCAAG GCCCCAAACG CTGTGGTGCT GGTGGTGGGA 
ACACATCTGG ACTTAATTGA AACCAAATTC CGTGTGGAGA GGATCGCCAC TCTACGCGCC 
TACGTGCTGG CCCTCTGCCG CTCGCCCTCT GGGTCCAGGG CCACCGGCTT CCCGGACATC 
ACGTGTAAAC ACCTGCACGA ACTCTCCTGT AAGAGCTTGG AAGGTCAGGA AGGGCTGCGG 
CAGCTCATTT TCCAGGTCAC GTGCAACATG AAGGACGTGG GCAGCACCAT CGGCTGTCAG 
CGACTTGCAG GGAGGCTGAT CCCCAGGAGC TACTTGAGCC TCCAGGAGGC TGTGCTGGCG 
GAGCAGCAGC GCCGCAGCCT GAGCGACGAC GTGCAGTACC TGACGGACCG GCAGCTGGAG 
CAGCTGGTAG ACCAGATGCC CTGCAGCGAC ATCCAGGACT ACGAGGATCT GCAGTCTGCC 
ATCAGCTTCC TCATTGAAAC GGGCTCCCTG CTGCACTTCC CGGACACCAG CCACGGCCTG 
AGGAACCTCT ACTTCCTAGA CCCCATCTGG CTGTCCGAGT GTCTGCAGAG GATCTTCAAC 
ATCAAGGGCT CTCGGTCAGT GGCCAAGAAC GGGGTGATCA GGGCCGAGGA CCTTCGGCTG 
CTGCTGGTTG GCACGGGCTT CACGAAGCAG ACAGAAGAGC AGTACTTCCA GTTCCTGGCC 
AAATTTGAGA TCGCCCTACC CGTTGCCAAT GACAGCTATC TCCTGCCACA TCTCCTTCCA 
TCCAAACCCG GCCTGGACAC CCACGGCATG CGGCACCCCA CAGCCAACAC CATTCAGAGA 
GTGTTTAAGA TGAGCTTCGT CCCGGTTGGC TTCTGGCAGA GGTTTATAGC GCGGATGCTG 
ATCAGCTTGG CCGAGATGGA CCTCCAGCTG TTTGAAAACA GGAAGAATAC TAAGAGCAGG 
CACAGGAAAG TCACCATTTA TAGTTTTACA GGAAACCAGA GAAATCGTTG CAGCACATTC 
AGAGTGAAAA GGAACCAGAC CATCTATTGG CAGGAGGGGC TCCTGGTCAC TTTTGATGGG 
GGCTACCTCA GTGTAGAATC CTCGGATGTG AACTGGAAAA AGAAAAAAAG TGGAGGAATA 
AAAATCATGT GCCAATCAGA AATAAGGGAC TTCTCGGCAA TGGCTTTTAT CACAGACCAT 
GTCAATTCCT TGATCGATCA GTGGTTTCCC GCCCTGACGG CCACGGAAAG TGACGGGACC 
CCACTGATGG AGCAGTATGT GCCCTGCCCT GTCTGCGAGG CCTCTTGGGC TCAGCACAGC 
AACCCCAGCG AGAGGTCAGA GGATGTGCAG TACTTCGACA TGGAGGACTG CGTGCTGACA 
GCCATTGAGC GGGACTTCAT CTCCTGCCCC AGGCACCCTG ACCTCCCGGT GCCTCTCCAG 
GAACTGGTCC CCGAGCTGTT CATGACCGAC TTCCCAGCCA GGCTCTTCTT GGAGAACAGC 
AAGCTGGAGC ACAGCGAGGA TGAGAGTGCT GTCCTGGGCC AAGGTGGGAG TGGCACCGTC 
ATCTACCGGG CCAGGTACCA AGGCCAGCCT GTGGCTGTGA AGCGATTCCA GATCAAGAAA 
TTCAAGAACT GTGCAAATGC CCCTGCAGAT ACCATGCTGA GGCACCTGCG GGCCACTGAC 
GCCATGAGGA ACTTCTCGGA GTTCCGGCAG GAGGCCAGCA TGCTGCACGC CCTGCAGCAC 
CCCTGCATCG TGTCCCTCAT CGGCATCAGC ATCCACCCGC TCTGCTTCGC GCTGGAGCTT 
GCCCCGCTGG GCAGCCTCAA CACCGTGCTG TCCGAGAACG CCAGAGATTC CTCCTTTATG 
CCCTTGGGAC ACATGCTCAC CCAAAAAATA GCCTACCAGA TAGCCTCTGG CCTGGCCTAC 
CTGCACAAGA AAAACATCAT CTTCTGTGAC CTGAAGTCCG ACAACATCCT GGTCTGGTCT 
CTGGACATCA AGGAGCACAT CAACATCAAG CTGTCCGACT ACGGCATCTC CCGGCAGTCA 
TTCCACGAGG GTGCCCTCGG CGTGGAGGGT ACTCCCGGCT ACCAGGCCCC GGAGATCCGG 
CCTCGCATCG TGTATGACGA GAAGGTAGAC ATGTTCTCCT ATGGGATGGT GCTCTACGAG 
TTGCTGTCAG GACAGCGGCC CGCACTGGGC CAGCACCAGC TTCAGATCTC CAAGAAGCTG 
TCCAAGGGCA TCCGCCCAGT TCTGGGTCAG CCGGAGGAAG TGCAGTTCCA CCGACTGCAG 
GCGCTTATGA TGGAGTGCTG GGATACGAAG CCCGAGAAGC GACCACTGGC CCTGGCGGTG 
GTGAGCCAAA TGAAGGACCC GACCTTTGCG ACCTTCATGT ACCAACTGCC CTGCGGGAAG 
CAGACATCCT TCTTCTCATC CCAGGGCCAG GAGTACATGG TGGTGTTCTG GGAAGGCAAG 
GAGGAGTCCA GGAACTACAC GGTGGTGAAC ACGGAGAAGG GCCTTCTGGA AGTGCAGCAC 
ATGAGCTGTG CGGGAATGAA GCTGAGCTGC CAACTCAAGA TCCAGAACTC GCTGTGGACG 
GCCACCGAGG ACCAGAAGAT CTACATCTAC AGCCTCAAGG GCATGTGTCC ACTGAACACA 
CCCCAGCGGG CTTTGGACAC CCCGGCCATC GTCACCTGCT TCTTGGCGGT ACCTGTTATA 
AAAAAGAACT CCCACCTGGT GCTGGCAGGC CTAGCGGACG GCCTGGTGGC GGTGTTCCCG 
GCAGCGTGGG GCGCCCCGGA CTACGGCTGC TCCTACCTGT GCTCGCACAC AGCCAACAGG 
GCCAAGTTCA GCATCCCCGA CGAGGACGCC CGGCAGAACC CCTACCCCGT GACGGCCATG 
GAGGTGGTCA ACTGCGGGTC CGAGGTCTGG TACAGCAACG GGCCAGGCCT GCTGGTCATT 
GACTGTGCGG CCCTGGAGAT CCGCCGGCGG CTGGAGCCCT ACGCAGCGCC CTCGGTGGTC 
ACATCTGTGG TGTGTGGCTC GGAGTGCCGT GGCGAGGAGG TGGTGTGGTG CCTGGACGAT 
AGGGCCAACT GCCTGGTGCT ATACCACTCG GCGACCTACC AGCTGTGCGC CCGCTACTTC 
TGCGGGGACC CCAGCCCCCT AGGGGACGTG TTTCCCGTGT GCCCGATGGG CCCACCACCC 
CTGGACAGCC CCACAGCCAG CGCGAGGGAG CCCGCGAGGG GCTCCATCGC CGACGTGAGC 
TTCATGTTCA GCGAGGAGCT GGGCACGCAG ATCCTCACCC ACCAGGACTC GCTGACCGAC 
TACTGTTCCC TATCCTCCTG CTCCGCGGCC TCGCCACACC CGGCACCCAT GGCCCTGTCC 
AGCCTGCCCT GCTCCCCGGC CAGCTCTGCC AGCTTGCCCT TCTCCTCTGA CTGCGAGGAC 
ACCAGCCAGC TGCCCAAGCC CACTGTGGGC TCCGACAGGT CGGTGCATGA CCTGAGCCCA 
GTGGACGGCG AGGCGTTCAG CCAGCACCTG CAGGCTGTGA AGATCCTTGC CGTGAAAGAC 
GTCATCTGGG TCCCCAGGCG CGGTGGAGAT ATCATCGTCA TTAGCCTGGA GAAGGAGGCA 
GGAACTCAGC GGGGCCACGT GATTGCTGTC TTGAAAGCCC GAGAGCTGAC TCCGCACGGG 
GTTCTCACGG AGGCTGCCGT GGTGGCAAAG GACACAGTTG TGTGCGGCTT TGAGAATGAA 
AATCTGGAGT GGTGCCTGGC TGTCTGGAGG GGCTGGGGCA TGCAGGAGTT TGACATTTTC 
TACCAGTCCT ACGAGGAGCT GGGCCGGCTG GAGGCCTGCG TGCGGAAGAG GAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_025271635.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127420.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

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CloneID	OBu57062
Clone ID Related Accession (Same CDS sequence)	XM_025271635.1
Accession Version	XM_025271635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACTC GGGAGCCAGA GGCTCTTCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCCGTCATC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGATAACA AACTGACAGA GCTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTCAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACCTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AACTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAC 
CACTTTGGCA AAAACGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTCG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC ACCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAGCTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
GTCAATGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACGGACAAG GCCCTGTATG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCGGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAACGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGT 
GTGGAGAGGA TCGCCACTCT ACGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTGGCGGAG CAGCAGCGCC GCAGCCTGAG CGACGACGTG 
CAGTACCTGA CGGACCGGCA GCTGGAGCAG CTGGTAGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGATCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTAGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAACGGG 
GTGATCAGGG CCGAGGACCT TCGGCTGCTG CTGGTTGGCA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTACCCGT TGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCCGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGAGGT TTATAGCGCG GATGCTGATC AGCTTGGCCG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACTTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGTGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CTTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA TGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCCGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGGC TCTTCTTGGA GAACAGCAAG CTGGAGCACA GCGAGGATGA GAGTGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTGTG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAACT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCGCT GGAGCTTGCC CCGCTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAAA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATCAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCATTC CACGAGGGTG CCCTCGGCGT GGAGGGTACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT CTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTTC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAAATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG CGGGAAGCAG ACATCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCGGTACC TGTTATAAAA AAGAACTCCC ACCTGGTGCT GGCAGGCCTA 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCA GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCCT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CAGCGCCCTC GGTGGTCACA TCTGTGGTGT GTGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTATA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCACCCCTG GACAGCCCCA CAGCCAGCGC GAGGGAGCCC 
GCGAGGGGCT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACGCAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCACACCCGG CACCCATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG CGAGGACACC AGCCAGCTGC CCAAGCCCAC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTTGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCACGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCACGGGGTT CTCACGGAGG CTGCCGTGGT GGCAAAGGAC 
ACAGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCATGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGCTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127420.1
CDS	1141..6444
Translation	MMTREPEALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271635.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271635.1

ORF Insert Sequence:

ATGATGACTC GGGAGCCAGA GGCTCTTCGT GTGAAGTGGT CCCACCTCAA GCTGCCCTGG 
GTGGATCTGG ACTGGCTCAT CGACATCTCC TGCCAGATCA CAGAGCTGGA TCTTTCTGCC 
AACTGCCTGG CGTCCCTCCC CTCCGTCATC CCCTGGGGCC TCATCAACCT CCGGAAGCTG 
AACCTCTCAG ACAACCACCT GGGGGAGCTG CCCAGCGTGC AGTCATCAGA TGAAATCATC 
TGTTCCAGGC TACTTGAAAT TGACATTTCC AGCAACAAGT TGTCCCACCT CCCACCAGGA 
TTCCTGCATC TCTCAAAACT TCAAAAACTG ACGGCTTCAA AAAACTATTT GGAAAAATTG 
TTTGAAGAAG AGAGTGCCAC GAACTGGATT GGTTTACGGA AGCTACAGGA GCTCGATGTT 
TCTGATAACA AACTGACAGA GCTCCCTGCA CTTTTCCTCC ATTCTTTCAA ATCGCTCAGT 
TGTCTCAACG TCTCCAGAAA TAACCTGAAG GCGTTTCCAG ATGCCTGGGC TTGCCCTTTG 
AAATGTTGCA AAGCATCCAA AAATGCCCTG GAGTCTCTAC CAGACAAAAT GGCTGTGTTT 
TGGAAGAACC ACCTGAAGGA TGTGGACTTC TCAGAAAACG CACTGAAAGA AGTTCCCCTG 
GGACTTTTCC AACTTGATGC CCTCATGTTC TTGAAGTTAC AGGGAAACCA GCTGGTGGCA 
CTCCCACCTC AAGAGAAGTG GACCTGCAGA CAGCTCAAAA CCCTGGATCT CTCCAGAAAC 
CACTTTGGCA AAAACGAAGA CGTCCTTAAA ACAAAGCGCA TTTCCTTTTT CACCACCAGA 
GGCCGCCAGC GTTCGGGGAC GGAGGCAGCG TGTGTACTCG AATTTCCCGC CTTCCTAAGT 
GAGTCTTTGG AAGTCCTCTG TCTGAACGAC AACCACCTGG ATGCAGTCCC ACCCTCAGTT 
TGCCTACTGA AGAGCTTATC AGAGCTCTAC TTGGGGAACA ATCCTAGCCT CCGAGAGCTC 
CCTCCTGAGC TGGGGCAGCT GGGGAACCTC TGGCAGTTGG ACATTGAAGA CCTGAATATC 
GTCAATGTGC CTGCAGAGAT CAGAAAAGAA GGCCCCAAAG CAATGCTGTC TTACCTGCGT 
GCTCAGCTGC GGAAAGCAGA GAAGTGCAAG CAGATGAAGA TGATCATCGT GGGTCCGCCG 
CGTCAGGGCA AGTCCACCCT CCTGGAGATC TTACAGACGG GGAGGGCCCC CCAGGTGGTA 
CACAGCGAGG CCACCATCAG GACCACCAAG TGGGAGCTGC AGAGGCCGGC TGGCTCGAGA 
GCCAAGGTTG AGTCTGTGGA GTTCACCGTC TGGGACATCG GCGGCCCCGC CAGCATGGCC 
ACGGTCAACC AGTGCTTCTT CACGGACAAG GCCCTGTATG TGGTGGTCTG GAACCTGGCT 
CTGGGGGAGG AGGCGGTGGC CAGCCTTCAG TTCTGGCTGC TCAACATCGA GGCCAAGGCC 
CCAAACGCTG TGGTGCTGGT GGTGGGAACA CATCTGGACT TAATTGAAAC CAAATTCCGT 
GTGGAGAGGA TCGCCACTCT ACGCGCCTAC GTGCTGGCCC TCTGCCGCTC GCCCTCTGGG 
TCCAGGGCCA CCGGCTTCCC GGACATCACG TGTAAACACC TGCACGAACT CTCCTGTAAG 
AGCTTGGAAG GTCAGGAAGG GCTGCGGCAG CTCATTTTCC AGGTCACGTG CAACATGAAG 
GACGTGGGCA GCACCATCGG CTGTCAGCGA CTTGCAGGGA GGCTGATCCC CAGGAGCTAC 
TTGAGCCTCC AGGAGGCTGT GCTGGCGGAG CAGCAGCGCC GCAGCCTGAG CGACGACGTG 
CAGTACCTGA CGGACCGGCA GCTGGAGCAG CTGGTAGACC AGATGCCCTG CAGCGACATC 
CAGGACTACG AGGATCTGCA GTCTGCCATC AGCTTCCTCA TTGAAACGGG CTCCCTGCTG 
CACTTCCCGG ACACCAGCCA CGGCCTGAGG AACCTCTACT TCCTAGACCC CATCTGGCTG 
TCCGAGTGTC TGCAGAGGAT CTTCAACATC AAGGGCTCTC GGTCAGTGGC CAAGAACGGG 
GTGATCAGGG CCGAGGACCT TCGGCTGCTG CTGGTTGGCA CGGGCTTCAC GAAGCAGACA 
GAAGAGCAGT ACTTCCAGTT CCTGGCCAAA TTTGAGATCG CCCTACCCGT TGCCAATGAC 
AGCTATCTCC TGCCACATCT CCTTCCATCC AAACCCGGCC TGGACACCCA CGGCATGCGG 
CACCCCACAG CCAACACCAT TCAGAGAGTG TTTAAGATGA GCTTCGTCCC GGTTGGCTTC 
TGGCAGAGGT TTATAGCGCG GATGCTGATC AGCTTGGCCG AGATGGACCT CCAGCTGTTT 
GAAAACAGGA AGAATACTAA GAGCAGGCAC AGGAAAGTCA CCATTTATAG TTTTACAGGA 
AACCAGAGAA ATCGTTGCAG CACATTCAGA GTGAAAAGGA ACCAGACCAT CTATTGGCAG 
GAGGGGCTCC TGGTCACTTT TGATGGGGGC TACCTCAGTG TAGAATCCTC GGATGTGAAC 
TGGAAAAAGA AAAAAAGTGG AGGAATAAAA ATCATGTGCC AATCAGAAAT AAGGGACTTC 
TCGGCAATGG CTTTTATCAC AGACCATGTC AATTCCTTGA TCGATCAGTG GTTTCCCGCC 
CTGACGGCCA CGGAAAGTGA CGGGACCCCA CTGATGGAGC AGTATGTGCC CTGCCCTGTC 
TGCGAGGCCT CTTGGGCTCA GCACAGCAAC CCCAGCGAGA GGTCAGAGGA TGTGCAGTAC 
TTCGACATGG AGGACTGCGT GCTGACAGCC ATTGAGCGGG ACTTCATCTC CTGCCCCAGG 
CACCCTGACC TCCCGGTGCC TCTCCAGGAA CTGGTCCCCG AGCTGTTCAT GACCGACTTC 
CCAGCCAGGC TCTTCTTGGA GAACAGCAAG CTGGAGCACA GCGAGGATGA GAGTGCTGTC 
CTGGGCCAAG GTGGGAGTGG CACCGTCATC TACCGGGCCA GGTACCAAGG CCAGCCTGTG 
GCTGTGAAGC GATTCCAGAT CAAGAAATTC AAGAACTGTG CAAATGCCCC TGCAGATACC 
ATGCTGAGGC ACCTGCGGGC CACTGACGCC ATGAGGAACT TCTCGGAGTT CCGGCAGGAG 
GCCAGCATGC TGCACGCCCT GCAGCACCCC TGCATCGTGT CCCTCATCGG CATCAGCATC 
CACCCGCTCT GCTTCGCGCT GGAGCTTGCC CCGCTGGGCA GCCTCAACAC CGTGCTGTCC 
GAGAACGCCA GAGATTCCTC CTTTATGCCC TTGGGACACA TGCTCACCCA AAAAATAGCC 
TACCAGATAG CCTCTGGCCT GGCCTACCTG CACAAGAAAA ACATCATCTT CTGTGACCTG 
AAGTCCGACA ACATCCTGGT CTGGTCTCTG GACATCAAGG AGCACATCAA CATCAAGCTG 
TCCGACTACG GCATCTCCCG GCAGTCATTC CACGAGGGTG CCCTCGGCGT GGAGGGTACT 
CCCGGCTACC AGGCCCCGGA GATCCGGCCT CGCATCGTGT ATGACGAGAA GGTAGACATG 
TTCTCCTATG GGATGGTGCT CTACGAGTTG CTGTCAGGAC AGCGGCCCGC ACTGGGCCAG 
CACCAGCTTC AGATCTCCAA GAAGCTGTCC AAGGGCATCC GCCCAGTTCT GGGTCAGCCG 
GAGGAAGTGC AGTTCCACCG ACTGCAGGCG CTTATGATGG AGTGCTGGGA TACGAAGCCC 
GAGAAGCGAC CACTGGCCCT GGCGGTGGTG AGCCAAATGA AGGACCCGAC CTTTGCGACC 
TTCATGTACC AACTGCCCTG CGGGAAGCAG ACATCCTTCT TCTCATCCCA GGGCCAGGAG 
TACATGGTGG TGTTCTGGGA AGGCAAGGAG GAGTCCAGGA ACTACACGGT GGTGAACACG 
GAGAAGGGCC TTCTGGAAGT GCAGCACATG AGCTGTGCGG GAATGAAGCT GAGCTGCCAA 
CTCAAGATCC AGAACTCGCT GTGGACGGCC ACCGAGGACC AGAAGATCTA CATCTACAGC 
CTCAAGGGCA TGTGTCCACT GAACACACCC CAGCGGGCTT TGGACACCCC GGCCATCGTC 
ACCTGCTTCT TGGCGGTACC TGTTATAAAA AAGAACTCCC ACCTGGTGCT GGCAGGCCTA 
GCGGACGGCC TGGTGGCGGT GTTCCCGGCA GCGTGGGGCG CCCCGGACTA CGGCTGCTCC 
TACCTGTGCT CGCACACAGC CAACAGGGCC AAGTTCAGCA TCCCCGACGA GGACGCCCGG 
CAGAACCCCT ACCCCGTGAC GGCCATGGAG GTGGTCAACT GCGGGTCCGA GGTCTGGTAC 
AGCAACGGGC CAGGCCTGCT GGTCATTGAC TGTGCGGCCC TGGAGATCCG CCGGCGGCTG 
GAGCCCTACG CAGCGCCCTC GGTGGTCACA TCTGTGGTGT GTGGCTCGGA GTGCCGTGGC 
GAGGAGGTGG TGTGGTGCCT GGACGATAGG GCCAACTGCC TGGTGCTATA CCACTCGGCG 
ACCTACCAGC TGTGCGCCCG CTACTTCTGC GGGGACCCCA GCCCCCTAGG GGACGTGTTT 
CCCGTGTGCC CGATGGGCCC ACCACCCCTG GACAGCCCCA CAGCCAGCGC GAGGGAGCCC 
GCGAGGGGCT CCATCGCCGA CGTGAGCTTC ATGTTCAGCG AGGAGCTGGG CACGCAGATC 
CTCACCCACC AGGACTCGCT GACCGACTAC TGTTCCCTAT CCTCCTGCTC CGCGGCCTCG 
CCACACCCGG CACCCATGGC CCTGTCCAGC CTGCCCTGCT CCCCGGCCAG CTCTGCCAGC 
TTGCCCTTCT CCTCTGACTG CGAGGACACC AGCCAGCTGC CCAAGCCCAC TGTGGGCTCC 
GACAGGTCGG TGCATGACCT GAGCCCAGTG GACGGCGAGG CGTTCAGCCA GCACCTGCAG 
GCTGTGAAGA TCCTTGCCGT GAAAGACGTC ATCTGGGTCC CCAGGCGCGG TGGAGATATC 
ATCGTCATTA GCCTGGAGAA GGAGGCAGGA ACTCAGCGGG GCCACGTGAT TGCTGTCTTG 
AAAGCCCGAG AGCTGACTCC GCACGGGGTT CTCACGGAGG CTGCCGTGGT GGCAAAGGAC 
ACAGTTGTGT GCGGCTTTGA GAATGAAAAT CTGGAGTGGT GCCTGGCTGT CTGGAGGGGC 
TGGGGCATGC AGGAGTTTGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGCTGGAG 
GCCTGCGTGC GGAAGAGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_006070641.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006070703.1 Bubalus bubalis leucine-rich repeat kinase 1 (LRRK1), mRNA.

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CloneID	OBu25198
Clone ID Related Accession (Same CDS sequence)	XM_006070641.1
Accession Version	XM_006070641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785399.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG ACAGGAACTC CCGTGGGTTT 
TCTCAGACCC CTTCTCTCCG CAGGGTTCTC ACGGAGGCTG CCGTGGTGGC AAAGGACACA 
GTTGTGTGCG GCTTTGAGAA TGAAAATCTG GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG 
GGCATGCAGG AGTTTGACAT TTTCTACCAG TCCTACGAGG AGCTGGGCCG GCTGGAGGCC 
TGCGTGCGGA AGAGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006070703.1
CDS	154..6174
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLDRNSRGFSQTPSLRRVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_006070641.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine-rich repeat kinase 1 (LRRK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006070641.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG ACAGGAACTC CCGTGGGTTT 
TCTCAGACCC CTTCTCTCCG CAGGGTTCTC ACGGAGGCTG CCGTGGTGGC AAAGGACACA 
GTTGTGTGCG GCTTTGAGAA TGAAAATCTG GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG 
GGCATGCAGG AGTTTGACAT TTTCTACCAG TCCTACGAGG AGCTGGGCCG GCTGGAGGCC 
TGCGTGCGGA AGAGGAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_025271631.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127416.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

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CloneID	OBu57060
Clone ID Related Accession (Same CDS sequence)	XM_025271631.1 , XM_025271629.1 , XM_025271632.1 , XM_025271633.1
Accession Version	XM_025271631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127416.1
CDS	295..6342
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271631.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271631.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_025271629.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127414.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

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CloneID	OBu57060
Clone ID Related Accession (Same CDS sequence)	XM_025271631.1 , XM_025271629.1 , XM_025271632.1 , XM_025271633.1
Accession Version	XM_025271629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127414.1
CDS	304..6351
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271629.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271629.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_025271632.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127417.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

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CloneID	OBu57060
Clone ID Related Accession (Same CDS sequence)	XM_025271631.1 , XM_025271629.1 , XM_025271632.1 , XM_025271633.1
Accession Version	XM_025271632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127417.1
CDS	580..6627
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271632.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271632.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK1 ( XM_025271633.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025127418.1 Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

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CloneID	OBu57060
Clone ID Related Accession (Same CDS sequence)	XM_025271631.1 , XM_025271629.1 , XM_025271632.1 , XM_025271633.1
Accession Version	XM_025271633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037564.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025127418.1
CDS	1..6048
Translation	MAGMSRLPPSMYWCVGPAGSAVCPGRAMETQENGAGDMGSKLSMPGSEPMVQSPRTEGIHAAYRRGDISRAQHLLREACDESTSQLEKGQLLSISAAHGDLETVRYLLTERLVELPTEPTDDNPAVVAAQFGHTDVVQELLESLPGPCSPQRLLNWMLALACQQGHLAVVKLLVLTYGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLIRHGAFFCSYILLDSPDPSKHLLRKYFVEASALPSSCPGKMALRVKWSHLKLPWVDLDWLIDISCQITELDLSANCLASLPSVIPWGLINLRKLNLSDNHLGELPSVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLEKLFEEESATNWIGLRKLQELDVSDNKLTELPALFLHSFKSLSCLNVSRNNLKAFPDAWACPLKCCKASKNALESLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLKLQGNQLVALPPQEKWTCRQLKTLDLSRNHFGKNEDVLKTKRISFFTTRGRQRSGTEAACVLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKSLSELYLGNNPSLRELPPELGQLGNLWQLDIEDLNIVNVPAEIRKEGPKAMLSYLRAQLRKAEKCKQMKMIIVGPPRQGKSTLLEILQTGRAPQVVHSEATIRTTKWELQRPAGSRAKVESVEFTVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVASLQFWLLNIEAKAPNAVVLVVGTHLDLIETKFRVERIATLRAYVLALCRSPSGSRATGFPDITCKHLHELSCKSLEGQEGLRQLIFQVTCNMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSLSDDVQYLTDRQLEQLVDQMPCSDIQDYEDLQSAISFLIETGSLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRLLLVGTGFTKQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENRKNTKSRHRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIMCQSEIRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHSNPSERSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDESAVLGQGGSGTVIYRARYQGQPVAVKRFQIKKFKNCANAPADTMLRHLRATDAMRNFSEFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENARDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDIKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGQHQLQISKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALAVVSQMKDPTFATFMYQLPCGKQTSFFSSQGQEYMVVFWEGKEESRNYTVVNTEKGLLEVQHMSCAGMKLSCQLKIQNSLWTATEDQKIYIYSLKGMCPLNTPQRALDTPAIVTCFLAVPVIKKNSHLVLAGLADGLVAVFPAAWGAPDYGCSYLCSHTANRAKFSIPDEDARQNPYPVTAMEVVNCGSEVWYSNGPGLLVIDCAALEIRRRLEPYAAPSVVTSVVCGSECRGEEVVWCLDDRANCLVLYHSATYQLCARYFCGDPSPLGDVFPVCPMGPPPLDSPTASAREPARGSIADVSFMFSEELGTQILTHQDSLTDYCSLSSCSAASPHPAPMALSSLPCSPASSASLPFSSDCEDTSQLPKPTVGSDRSVHDLSPVDGEAFSQHLQAVKILAVKDVIWVPRRGGDIIVISLEKEAGTQRGHVIAVLKARELTPHGVLTEAAVVAKDTVVCGFENENLEWCLAVWRGWGMQEFDIFYQSYEELGRLEACVRKRR

Target ORF information:

RefSeq Version	XM_025271633.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis leucine rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025271633.1

ORF Insert Sequence:

ATGGCTGGAA TGTCTCGGCT ACCGCCCAGC ATGTACTGGT GTGTGGGGCC AGCAGGGTCA 
GCTGTGTGTC CAGGACGCGC CATGGAGACC CAGGAGAACG GTGCTGGGGA CATGGGCAGC 
AAACTCTCCA TGCCAGGCAG TGAGCCCATG GTGCAGAGCC CCAGGACGGA AGGCATTCAT 
GCTGCGTACA GGCGAGGAGA CATCAGCAGA GCCCAGCATC TGCTCCGAGA AGCTTGTGAC 
GAGAGCACAT CCCAGCTGGA AAAGGGCCAG CTCCTGAGTA TCTCTGCAGC CCATGGTGAC 
CTGGAGACCG TCCGGTATCT TCTCACTGAG AGGCTGGTGG AGCTACCGAC GGAGCCCACT 
GACGACAACC CTGCCGTGGT TGCAGCACAG TTTGGGCACA CTGATGTCGT CCAGGAGCTA 
CTCGAGTCCT TGCCAGGTCC CTGCAGCCCC CAGCGGCTCT TGAATTGGAT GCTGGCCTTG 
GCCTGCCAGC AGGGGCACCT GGCAGTGGTG AAGCTCCTGG TCCTGACCTA CGGGGCCGAC 
CCGGAGAGCT ACGCAGTCAG GAAGAACGAG TTCCCTGTCA TCGTGCGCCT GCCGCTGTAC 
GCGGCCATCA AGTCAGGGAA TGAAGATATT GCCATCTTCC TGATTCGGCA TGGAGCCTTT 
TTCTGCTCCT ACATTTTACT GGACAGTCCT GACCCCAGCA AACATCTCCT CAGGAAGTAT 
TTTGTTGAAG CAAGCGCCTT GCCCAGCAGT TGTCCGGGAA AAATGGCTCT TCGTGTGAAG 
TGGTCCCACC TCAAGCTGCC CTGGGTGGAT CTGGACTGGC TCATCGACAT CTCCTGCCAG 
ATCACAGAGC TGGATCTTTC TGCCAACTGC CTGGCGTCCC TCCCCTCCGT CATCCCCTGG 
GGCCTCATCA ACCTCCGGAA GCTGAACCTC TCAGACAACC ACCTGGGGGA GCTGCCCAGC 
GTGCAGTCAT CAGATGAAAT CATCTGTTCC AGGCTACTTG AAATTGACAT TTCCAGCAAC 
AAGTTGTCCC ACCTCCCACC AGGATTCCTG CATCTCTCAA AACTTCAAAA ACTGACGGCT 
TCAAAAAACT ATTTGGAAAA ATTGTTTGAA GAAGAGAGTG CCACGAACTG GATTGGTTTA 
CGGAAGCTAC AGGAGCTCGA TGTTTCTGAT AACAAACTGA CAGAGCTCCC TGCACTTTTC 
CTCCATTCTT TCAAATCGCT CAGTTGTCTC AACGTCTCCA GAAATAACCT GAAGGCGTTT 
CCAGATGCCT GGGCTTGCCC TTTGAAATGT TGCAAAGCAT CCAAAAATGC CCTGGAGTCT 
CTACCAGACA AAATGGCTGT GTTTTGGAAG AACCACCTGA AGGATGTGGA CTTCTCAGAA 
AACGCACTGA AAGAAGTTCC CCTGGGACTT TTCCAACTTG ATGCCCTCAT GTTCTTGAAG 
TTACAGGGAA ACCAGCTGGT GGCACTCCCA CCTCAAGAGA AGTGGACCTG CAGACAGCTC 
AAAACCCTGG ATCTCTCCAG AAACCACTTT GGCAAAAACG AAGACGTCCT TAAAACAAAG 
CGCATTTCCT TTTTCACCAC CAGAGGCCGC CAGCGTTCGG GGACGGAGGC AGCGTGTGTA 
CTCGAATTTC CCGCCTTCCT AAGTGAGTCT TTGGAAGTCC TCTGTCTGAA CGACAACCAC 
CTGGATGCAG TCCCACCCTC AGTTTGCCTA CTGAAGAGCT TATCAGAGCT CTACTTGGGG 
AACAATCCTA GCCTCCGAGA GCTCCCTCCT GAGCTGGGGC AGCTGGGGAA CCTCTGGCAG 
TTGGACATTG AAGACCTGAA TATCGTCAAT GTGCCTGCAG AGATCAGAAA AGAAGGCCCC 
AAAGCAATGC TGTCTTACCT GCGTGCTCAG CTGCGGAAAG CAGAGAAGTG CAAGCAGATG 
AAGATGATCA TCGTGGGTCC GCCGCGTCAG GGCAAGTCCA CCCTCCTGGA GATCTTACAG 
ACGGGGAGGG CCCCCCAGGT GGTACACAGC GAGGCCACCA TCAGGACCAC CAAGTGGGAG 
CTGCAGAGGC CGGCTGGCTC GAGAGCCAAG GTTGAGTCTG TGGAGTTCAC CGTCTGGGAC 
ATCGGCGGCC CCGCCAGCAT GGCCACGGTC AACCAGTGCT TCTTCACGGA CAAGGCCCTG 
TATGTGGTGG TCTGGAACCT GGCTCTGGGG GAGGAGGCGG TGGCCAGCCT TCAGTTCTGG 
CTGCTCAACA TCGAGGCCAA GGCCCCAAAC GCTGTGGTGC TGGTGGTGGG AACACATCTG 
GACTTAATTG AAACCAAATT CCGTGTGGAG AGGATCGCCA CTCTACGCGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCCTC TGGGTCCAGG GCCACCGGCT TCCCGGACAT CACGTGTAAA 
CACCTGCACG AACTCTCCTG TAAGAGCTTG GAAGGTCAGG AAGGGCTGCG GCAGCTCATT 
TTCCAGGTCA CGTGCAACAT GAAGGACGTG GGCAGCACCA TCGGCTGTCA GCGACTTGCA 
GGGAGGCTGA TCCCCAGGAG CTACTTGAGC CTCCAGGAGG CTGTGCTGGC GGAGCAGCAG 
CGCCGCAGCC TGAGCGACGA CGTGCAGTAC CTGACGGACC GGCAGCTGGA GCAGCTGGTA 
GACCAGATGC CCTGCAGCGA CATCCAGGAC TACGAGGATC TGCAGTCTGC CATCAGCTTC 
CTCATTGAAA CGGGCTCCCT GCTGCACTTC CCGGACACCA GCCACGGCCT GAGGAACCTC 
TACTTCCTAG ACCCCATCTG GCTGTCCGAG TGTCTGCAGA GGATCTTCAA CATCAAGGGC 
TCTCGGTCAG TGGCCAAGAA CGGGGTGATC AGGGCCGAGG ACCTTCGGCT GCTGCTGGTT 
GGCACGGGCT TCACGAAGCA GACAGAAGAG CAGTACTTCC AGTTCCTGGC CAAATTTGAG 
ATCGCCCTAC CCGTTGCCAA TGACAGCTAT CTCCTGCCAC ATCTCCTTCC ATCCAAACCC 
GGCCTGGACA CCCACGGCAT GCGGCACCCC ACAGCCAACA CCATTCAGAG AGTGTTTAAG 
ATGAGCTTCG TCCCGGTTGG CTTCTGGCAG AGGTTTATAG CGCGGATGCT GATCAGCTTG 
GCCGAGATGG ACCTCCAGCT GTTTGAAAAC AGGAAGAATA CTAAGAGCAG GCACAGGAAA 
GTCACCATTT ATAGTTTTAC AGGAAACCAG AGAAATCGTT GCAGCACATT CAGAGTGAAA 
AGGAACCAGA CCATCTATTG GCAGGAGGGG CTCCTGGTCA CTTTTGATGG GGGCTACCTC 
AGTGTAGAAT CCTCGGATGT GAACTGGAAA AAGAAAAAAA GTGGAGGAAT AAAAATCATG 
TGCCAATCAG AAATAAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAATTCC 
TTGATCGATC AGTGGTTTCC CGCCCTGACG GCCACGGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTATG TGCCCTGCCC TGTCTGCGAG GCCTCTTGGG CTCAGCACAG CAACCCCAGC 
GAGAGGTCAG AGGATGTGCA GTACTTCGAC ATGGAGGACT GCGTGCTGAC AGCCATTGAG 
CGGGACTTCA TCTCCTGCCC CAGGCACCCT GACCTCCCGG TGCCTCTCCA GGAACTGGTC 
CCCGAGCTGT TCATGACCGA CTTCCCAGCC AGGCTCTTCT TGGAGAACAG CAAGCTGGAG 
CACAGCGAGG ATGAGAGTGC TGTCCTGGGC CAAGGTGGGA GTGGCACCGT CATCTACCGG 
GCCAGGTACC AAGGCCAGCC TGTGGCTGTG AAGCGATTCC AGATCAAGAA ATTCAAGAAC 
TGTGCAAATG CCCCTGCAGA TACCATGCTG AGGCACCTGC GGGCCACTGA CGCCATGAGG 
AACTTCTCGG AGTTCCGGCA GGAGGCCAGC ATGCTGCACG CCCTGCAGCA CCCCTGCATC 
GTGTCCCTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CGCTGGAGCT TGCCCCGCTG 
GGCAGCCTCA ACACCGTGCT GTCCGAGAAC GCCAGAGATT CCTCCTTTAT GCCCTTGGGA 
CACATGCTCA CCCAAAAAAT AGCCTACCAG ATAGCCTCTG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTGAAGTCC GACAACATCC TGGTCTGGTC TCTGGACATC 
AAGGAGCACA TCAACATCAA GCTGTCCGAC TACGGCATCT CCCGGCAGTC ATTCCACGAG 
GGTGCCCTCG GCGTGGAGGG TACTCCCGGC TACCAGGCCC CGGAGATCCG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA CATGTTCTCC TATGGGATGG TGCTCTACGA GTTGCTGTCA 
GGACAGCGGC CCGCACTGGG CCAGCACCAG CTTCAGATCT CCAAGAAGCT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGTCA GCCGGAGGAA GTGCAGTTCC ACCGACTGCA GGCGCTTATG 
ATGGAGTGCT GGGATACGAA GCCCGAGAAG CGACCACTGG CCCTGGCGGT GGTGAGCCAA 
ATGAAGGACC CGACCTTTGC GACCTTCATG TACCAACTGC CCTGCGGGAA GCAGACATCC 
TTCTTCTCAT CCCAGGGCCA GGAGTACATG GTGGTGTTCT GGGAAGGCAA GGAGGAGTCC 
AGGAACTACA CGGTGGTGAA CACGGAGAAG GGCCTTCTGG AAGTGCAGCA CATGAGCTGT 
GCGGGAATGA AGCTGAGCTG CCAACTCAAG ATCCAGAACT CGCTGTGGAC GGCCACCGAG 
GACCAGAAGA TCTACATCTA CAGCCTCAAG GGCATGTGTC CACTGAACAC ACCCCAGCGG 
GCTTTGGACA CCCCGGCCAT CGTCACCTGC TTCTTGGCGG TACCTGTTAT AAAAAAGAAC 
TCCCACCTGG TGCTGGCAGG CCTAGCGGAC GGCCTGGTGG CGGTGTTCCC GGCAGCGTGG 
GGCGCCCCGG ACTACGGCTG CTCCTACCTG TGCTCGCACA CAGCCAACAG GGCCAAGTTC 
AGCATCCCCG ACGAGGACGC CCGGCAGAAC CCCTACCCCG TGACGGCCAT GGAGGTGGTC 
AACTGCGGGT CCGAGGTCTG GTACAGCAAC GGGCCAGGCC TGCTGGTCAT TGACTGTGCG 
GCCCTGGAGA TCCGCCGGCG GCTGGAGCCC TACGCAGCGC CCTCGGTGGT CACATCTGTG 
GTGTGTGGCT CGGAGTGCCG TGGCGAGGAG GTGGTGTGGT GCCTGGACGA TAGGGCCAAC 
TGCCTGGTGC TATACCACTC GGCGACCTAC CAGCTGTGCG CCCGCTACTT CTGCGGGGAC 
CCCAGCCCCC TAGGGGACGT GTTTCCCGTG TGCCCGATGG GCCCACCACC CCTGGACAGC 
CCCACAGCCA GCGCGAGGGA GCCCGCGAGG GGCTCCATCG CCGACGTGAG CTTCATGTTC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTGACCGA CTACTGTTCC 
CTATCCTCCT GCTCCGCGGC CTCGCCACAC CCGGCACCCA TGGCCCTGTC CAGCCTGCCC 
TGCTCCCCGG CCAGCTCTGC CAGCTTGCCC TTCTCCTCTG ACTGCGAGGA CACCAGCCAG 
CTGCCCAAGC CCACTGTGGG CTCCGACAGG TCGGTGCATG ACCTGAGCCC AGTGGACGGC 
GAGGCGTTCA GCCAGCACCT GCAGGCTGTG AAGATCCTTG CCGTGAAAGA CGTCATCTGG 
GTCCCCAGGC GCGGTGGAGA TATCATCGTC ATTAGCCTGG AGAAGGAGGC AGGAACTCAG 
CGGGGCCACG TGATTGCTGT CTTGAAAGCC CGAGAGCTGA CTCCGCACGG GGTTCTCACG 
GAGGCTGCCG TGGTGGCAAA GGACACAGTT GTGTGCGGCT TTGAGAATGA AAATCTGGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC ATGCAGGAGT TTGACATTTT CTACCAGTCC 
TACGAGGAGC TGGGCCGGCT GGAGGCCTGC GTGCGGAAGA GGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LRRK1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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