ITCH cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITCH
Entrez Gene ID	102390921
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006048070.1	XP_006048132.1	E3 ubiquitin-protein ligase Itchy homolog
XM_025263616.1	XP_025119401.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_006048070.2	XP_006048132.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_025263617.1	XP_025119402.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu06574	XM_006048070.2 Latest version!	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu06574	XM_025263616.1 Latest version!	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu48170	XM_025263617.1 Latest version!	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OBu06574	XM_006048070.1	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITCH ( XM_006048070.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048132.1 Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), mRNA.

Price & Availability↑

CloneID	OBu06574
Clone ID Related Accession (Same CDS sequence)	XM_006048070.1 , XM_025263616.1 , XM_006048070.2
Accession Version	XM_006048070.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784085.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048132.1
CDS	204..2792
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVVTLQLVGDKEPAETIGDLSVCLDGLQLESEVVANGETTCSESASQNDDGSRPKDETRANASGSDDPEDTGSGENRRINGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNSSTSEGAASGLIIPLTISGGAGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_006048070.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048070.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_025263616.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025119401.1 Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu06574
Clone ID Related Accession (Same CDS sequence)	XM_006048070.1 , XM_025263616.1 , XM_006048070.2
Accession Version	XM_025263616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037558.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025119401.1
CDS	381..2969
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVVTLQLVGDKEPAETIGDLSVCLDGLQLESEVVANGETTCSESASQNDDGSRPKDETRANASGSDDPEDTGSGENRRINGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNSSTSEGAASGLIIPLTISGGAGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_025263616.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263616.1

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_006048070.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048132.1 Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu06574
Clone ID Related Accession (Same CDS sequence)	XM_006048070.1 , XM_025263616.1 , XM_006048070.2
Accession Version	XM_006048070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037558.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006048070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048132.1
CDS	244..2832
Translation	MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVVTLQLVGDKEPAETIGDLSVCLDGLQLESEVVANGETTCSESASQNDDGSRPKDETRANASGSDDPEDTGSGENRRINGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNSSTSEGAASGLIIPLTISGGAGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_006048070.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048070.2

ORF Insert Sequence:

ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT 
CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG 
AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT 
TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG 
GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA 
CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT 
GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTACATGTTC AGAAAGTGCT 
TCACAGAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCAAACGC AAGTGGATCA 
GATGACCCTG AGGATACAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA 
TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA 
CCTACCCCCC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT 
GGCTCTAGCA CAGGCTCTCT GCCACCAACG AATACAAATT CAAGTACATC TGAAGGAGCA 
GCATCTGGAT TAATCATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT 
CCTGTACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA 
GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT 
CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA 
AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG 
CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG 
AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG 
CCTGGATGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT 
CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT 
GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA 
AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG 
ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA 
CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC 
TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT 
CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA 
CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC 
TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT 
GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG 
CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT 
CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAAAACA ATATTGAGGA GTGTGGTTTG 
GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA 
CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA 
GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT 
AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGACG CAAAGGAATT AGAGGTTCTT 
TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC 
TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT 
GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA 
TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA 
GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG 
AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA 
CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITCH ( XM_025263617.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025119402.1 Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu48170
Clone ID Related Accession (Same CDS sequence)	XM_025263617.1
Accession Version	XM_025263617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037558.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTTG AGGAAGTAGT TGTGACTTTA CAGCTTGTAG GTGACAAAGA ACCAGCAGAG 
ACAATAGGAG ATTTGTCAGT CTGTCTTGAT GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCACAGAATG ATGATGGCTC CAGGCCCAAG 
GATGAAACAA GAGCAAACGC AAGTGGATCA GATGACCCTG AGGATACAGG GTCTGGTGAA 
AATAGGAGGA TCAATGGGAA TAATTCTCCA TCACTCTCAA ATGGCGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCCC GTAGACCAGC ATCTGTCAAT 
GGCTCACCAT CTACCACTTC TGAAAGTGAT GGCTCTAGCA CAGGCTCTCT GCCACCAACG 
AATACAAATT CAAGTACATC TGAAGGAGCA GCATCTGGAT TAATCATTCC TCTTACTATA 
TCTGGAGGTG CAGGCCCTAG ACCATTAAAT CCTGTACCTC AAGCTCCCCT CCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AGCGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCAGTCC GGAACTATGA ACAATGGCAG CTCCAGCGTA GTCAACTTCA GGGAGCTATG 
CAGCAGTTTA ACCAGAGGTT CATTTATGGG AATCAAGATT TGTTTGCTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTGGG TCCCCTGCCG CCTGGATGGG AGAAGAGAAC GGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAAT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACGGTAGAC 
GGAATTCCGT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATAGA TCCCCGCACC 
GGAAAATCTG CCTTAGACAA TGGACCCCAG ATAGCCTATG TACGGGACTT CAAGGCAAAG 
GTTCAATATT TCCGTTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAGCAGA TAATGAGCTT CAGTCCTCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATCTT GAATAAACCA 
GTTGGACTGA AGGATTTAGA ATCGATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA GTGTGGTTTG GAAATGTATT TCTCCGTTGA TAAAGAAATC 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCCAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GATTGTCTCG TGGTGTTGAA 
GAACAGACGC AAGCTTTCTT TGAGGGCTTT AATGAAATTC TTCCCCAGCA GTATTTACAG 
TACTTCGACG CAAAGGAATT AGAGGTTCTT TTATGTGGGA TGCAAGAGAT TGATTTGAAT 
GATTGGCAAA GACATGCCAT CTACCGTCAC TATACCAGGA CAAGTAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATCGATAAT GAGAAGAGAA TGCGACTTTT GCAGTTTGTT 
ACTGGGACCT GCCGACTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGGCCA 
CAGAAGTTCT GCATTGAAAA AGTCGGGAAA GAAAATTGGT TGCCTAGAAG TCATACCTGT 
TTTAACCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTA 
TTTGCTATTG AAGAAACAGA AGGCTTTGGA CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025119402.1
CDS	210..2468
Translation	MKLEEVVVTLQLVGDKEPAETIGDLSVCLDGLQLESEVVANGETTCSESASQNDDGSRPKDETRANASGSDDPEDTGSGENRRINGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNSSTSEGAASGLIIPLTISGGAGPRPLNPVPQAPLPPGWEQRVDQHGRVYYVDHIEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_025263617.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis itchy E3 ubiquitin protein ligase (ITCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263617.1

ORF Insert Sequence:

ATGAAACTTG AGGAAGTAGT TGTGACTTTA CAGCTTGTAG GTGACAAAGA ACCAGCAGAG 
ACAATAGGAG ATTTGTCAGT CTGTCTTGAT GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC 
AATGGTGAAA CTACATGTTC AGAAAGTGCT TCACAGAATG ATGATGGCTC CAGGCCCAAG 
GATGAAACAA GAGCAAACGC AAGTGGATCA GATGACCCTG AGGATACAGG GTCTGGTGAA 
AATAGGAGGA TCAATGGGAA TAATTCTCCA TCACTCTCAA ATGGCGGTTT TAAACCTTCT 
AGGCCTCCAA GACCTTCACG ACCACCTCCA CCTACCCCCC GTAGACCAGC ATCTGTCAAT 
GGCTCACCAT CTACCACTTC TGAAAGTGAT GGCTCTAGCA CAGGCTCTCT GCCACCAACG 
AATACAAATT CAAGTACATC TGAAGGAGCA GCATCTGGAT TAATCATTCC TCTTACTATA 
TCTGGAGGTG CAGGCCCTAG ACCATTAAAT CCTGTACCTC AAGCTCCCCT CCCACCTGGT 
TGGGAGCAGA GAGTGGACCA GCATGGGCGA GTTTACTATG TAGATCATAT TGAAAAAAGA 
ACAACGTGGG ATAGACCAGA ACCTCTACCT CCTGGCTGGG AGCGGCGGGT TGACAACATG 
GGACGTATTT ATTATGTTGA CCATTTCACA AGAACAACAA CGTGGCAGAG GCCAACACTG 
GAATCAGTCC GGAACTATGA ACAATGGCAG CTCCAGCGTA GTCAACTTCA GGGAGCTATG 
CAGCAGTTTA ACCAGAGGTT CATTTATGGG AATCAAGATT TGTTTGCTAC ATCACAAAAT 
AAAGAATTTG ATCCCCTGGG TCCCCTGCCG CCTGGATGGG AGAAGAGAAC GGACAGCAAT 
GGTAGAGTAT ATTTTGTCAA CCACAACACT CGTATTACAC AATGGGAAGA CCCCAGAAAT 
CAAGGTCAAT TAAATGAAAA GCCCTTACCT GAAGGCTGGG AAATGAGATT CACGGTAGAC 
GGAATTCCGT ATTTTGTGGA CCACAATAGA AGAACAACCA CCTATATAGA TCCCCGCACC 
GGAAAATCTG CCTTAGACAA TGGACCCCAG ATAGCCTATG TACGGGACTT CAAGGCAAAG 
GTTCAATATT TCCGTTTCTG GTGTCAGCAA CTGGCCATGC CACAGCACAT AAAGATTACA 
GTGACAAGAA AAACATTGTT TGAGGATTCC TTTCAGCAGA TAATGAGCTT CAGTCCTCAA 
GATCTGCGAA GACGTTTGTG GGTGATTTTT CCAGGAGAAG AAGGTTTAGA TTATGGAGGT 
GTAGCAAGAG AATGGTTCTT TCTTTTGTCA CATGAGGTGT TGAACCCAAT GTATTGCCTG 
TTTGAATATG CAGGGAAGGA TAACTACTGC TTGCAGATAA ACCCCGCTTC TTACATCAAT 
CCAGATCACC TGAAATATTT TCGTTTTATT GGCAGGTTTA TTGCTATGGC TCTGTTCCAT 
GGGAAATTCA TAGACACAGG TTTTTCTTTG CCATTCTATA AGCGTATCTT GAATAAACCA 
GTTGGACTGA AGGATTTAGA ATCGATTGAT CCAGAATTTT ACAATTCTCT CATCTGGGTT 
AAAGAAAACA ATATTGAGGA GTGTGGTTTG GAAATGTATT TCTCCGTTGA TAAAGAAATC 
TTAGGTGAAA TCAAGAGTCA TGATTTGAAA CCCAATGGTG GCAATATTCT TGTAACAGAA 
GAAAATAAAG AGGAATATAT CAGAATGGTA GCTGAATGGA GATTGTCTCG TGGTGTTGAA 
GAACAGACGC AAGCTTTCTT TGAGGGCTTT AATGAAATTC TTCCCCAGCA GTATTTACAG 
TACTTCGACG CAAAGGAATT AGAGGTTCTT TTATGTGGGA TGCAAGAGAT TGATTTGAAT 
GATTGGCAAA GACATGCCAT CTACCGTCAC TATACCAGGA CAAGTAAACA AATCATGTGG 
TTTTGGCAGT TTGTTAAAGA AATCGATAAT GAGAAGAGAA TGCGACTTTT GCAGTTTGTT 
ACTGGGACCT GCCGACTGCC AGTTGGAGGA TTTGCTGACC TCATGGGGAG CAATGGGCCA 
CAGAAGTTCT GCATTGAAAA AGTCGGGAAA GAAAATTGGT TGCCTAGAAG TCATACCTGT 
TTTAACCGCC TGGACCTGCC ACCCTACAAG AGCTATGAGC AACTGAAGGA AAAGCTGTTA 
TTTGCTATTG AAGAAACAGA AGGCTTTGGA CAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ITCH cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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