LOC103872484 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103872484
Entrez Gene ID	103872484
Full Name	ATP-dependent DNA helicase 2 subunit KU70
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009150886.2	XP_009149134.1	ATP-dependent DNA helicase 2 subunit KU70

Pathways from BioSystems
Gene Ontology

The following LOC103872484 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103872484 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr155031	XM_009150886.2 Latest version!	Brassica rapa ATP-dependent DNA helicase 2 subunit KU70 (LOC103872484), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103872484 ( XM_009150886.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009149134.1 Brassica rapa ATP-dependent DNA helicase 2 subunit KU70 (LOC103872484), mRNA.

Price & Availability↑

CloneID	OBr155031
Clone ID Related Accession (Same CDS sequence)	XM_009150886.2
Accession Version	XM_009150886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	ATP-dependent DNA helicase 2 subunit KU70
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024800.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009150886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ACCCAGAAGA TGTTTTCAGA GATGAAGACG AAGACCCAGA AAGCCAATTC 
TTCCAGGAAA AAGAAGCTTC CAAAGAGTTT GTAGTCTATC TAATCGACGC TTCTCCCAAA 
ATGTTCAGCT CTACTTGCCC CTCTGAGGAT GAGAAACAAG AAAGCCATTT TCACATTGCT 
GTTAGTTGCA TTGCTCAGTC ACTCAAGTCT CATATTATCA ACCGTTCTAA TGATGAAATT 
GCAATATGCT TCTTTAACAC TCGTGAAAAG AAGAATCTTC AAGACCTGAA TGGTGTTTAT 
GTTTTTAATG TTCCACAAAG AGAGTGTATT GACCGTCCAA CAGCTAGGCT GATTAAAGAC 
TTTGATCTCA TTGAAGAATC ATTCATAAAA GATATTGGGA GTCAAAATGG TATAGTCTCA 
GATTCTCGGG AGAATTCTCT TTATAGTGCT CTTTGGGTTG CACAAGCACT ACTGCGCAAA 
GGATCTTCAA AGACAGCAGA TAAGCGCATA TTTCTGTTTA CCAATGAAGA TGATCCTTTT 
GGGAGTATGA GGATTTCTGT AAAAGAGGAT ATGACAAGGA CTACATTGCA GAGAGCTAAG 
GATGCACAAG ACCTTGGCAT CTCAATCGAG CTTCTTCCCC TCAGTCACCC TGATAAGCAA 
TTCGATATCT CTCTCTTCTA CAAGGATTTA ATTGGATTAA ATGGTGATGA GCTTACGGAG 
TTCATGCCAT CAGTTGGTCA GAAGTTAGAG GACATGAAGG ATCAACTGAA GAAGCGTGTA 
CTTGCCAAGA GAATAGCAAA ACGAATAACG TTTATGATAT GTGATGGTGT TTCTATTGAA 
CTGAATGGTT ATGCTTTACT CCGTCCTGCT ACTCCTGGAA CCATAACCTG GCTTGACTCT 
ACTACTAATC TTCCTATAAA GGTGGAAAGA TCATATGTTT GTGCGGACAC TGGTGCGATA 
ATGCAAGAAC CTATTCAACG GATCCAGCCT TACATGAATC GGGATGTCAT GTTTACAGCG 
GCAGAGCTAT CCGAACTAAA AAAGATATCA ACTGGACATT TAAGACTGCT GGGGTTCAAG 
CCTCTTAGTT GCCTCAAAGA TTATCACAAC ATGAAGCCTT CTACCTTTCT CTATCCAAGC 
GATAAGGAAG TTATTGGGAG CACTCGTGCA TTCATTGCCT TGCATAGATC CATGATACAG 
CTTGGACGTT TTGCTGTTGC CTTTTATGGT GGTACGACAC CTCCTAGATT GGTTGCACTT 
GTTGCTCAGG ATGAAATAGA GAGTGATGGT GGTCAAGTGG AGCCACCAGG GATGAATATG 
ATATACCTTC CATATGCTAA TGATATTAGA GATATCGAAG AGTTGCATTC AAAACCTGGT 
GTAGCTGCTC CTCGAGCATC AGAAGATCAG CTCAAAAAGG CATCAGCTCT AATGCGACGC 
CTAGAGTTAA AGGACTTTTC TGTTTGCCAA TTTGCTAATC CCGCTTTACA GAGACACTAC 
GCGATGTTGC AAGCCATTGC ATTGGATGAA GACCAGCTTG GAGAAACAAG AGATGAGACT 
CTTCCTGACG AAGGAGGAAT GAACAGACCT GGAGTTGTAA AAGCAGTAGA AGAATTTAAA 
GAGTCTATTT ACGGTGATGA CTCTGAGGAA GAAAGTGACT CAGGGGCAAA GGAAAAATCA 
AGAAAGCGAA AAGCTGCAGA TGCAGGAGAC TATGACTTTG TCGAGCTTGC AAAAACCGGG 
AAGCTTAAAG ACTTGACGGT TGTGGAGCTG AAGACATACT TGACTGCCAA CAATCTTCCC 
CTCAGCGGGA AGAAAGATGC TCTCATCAAT AGGATTCTGG CTCACGTCAG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009149134.1
CDS	67..1923
Translation	MELDPEDVFRDEDEDPESQFFQEKEASKEFVVYLIDASPKMFSSTCPSEDEKQESHFHIAVSCIAQSLKSHIINRSNDEIAICFFNTREKKNLQDLNGVYVFNVPQRECIDRPTARLIKDFDLIEESFIKDIGSQNGIVSDSRENSLYSALWVAQALLRKGSSKTADKRIFLFTNEDDPFGSMRISVKEDMTRTTLQRAKDAQDLGISIELLPLSHPDKQFDISLFYKDLIGLNGDELTEFMPSVGQKLEDMKDQLKKRVLAKRIAKRITFMICDGVSIELNGYALLRPATPGTITWLDSTTNLPIKVERSYVCADTGAIMQEPIQRIQPYMNRDVMFTAAELSELKKISTGHLRLLGFKPLSCLKDYHNMKPSTFLYPSDKEVIGSTRAFIALHRSMIQLGRFAVAFYGGTTPPRLVALVAQDEIESDGGQVEPPGMNMIYLPYANDIRDIEELHSKPGVAAPRASEDQLKKASALMRRLELKDFSVCQFANPALQRHYAMLQAIALDEDQLGETRDETLPDEGGMNRPGVVKAVEEFKESIYGDDSEEESDSGAKEKSRKRKAADAGDYDFVELAKTGKLKDLTVVELKTYLTANNLPLSGKKDALINRILAHVSK

Target ORF information:

RefSeq Version	XM_009150886.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa ATP-dependent DNA helicase 2 subunit KU70 (LOC103872484), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009150886.2

ORF Insert Sequence:

ATGGAACTTG ACCCAGAAGA TGTTTTCAGA GATGAAGACG AAGACCCAGA AAGCCAATTC 
TTCCAGGAAA AAGAAGCTTC CAAAGAGTTT GTAGTCTATC TAATCGACGC TTCTCCCAAA 
ATGTTCAGCT CTACTTGCCC CTCTGAGGAT GAGAAACAAG AAAGCCATTT TCACATTGCT 
GTTAGTTGCA TTGCTCAGTC ACTCAAGTCT CATATTATCA ACCGTTCTAA TGATGAAATT 
GCAATATGCT TCTTTAACAC TCGTGAAAAG AAGAATCTTC AAGACCTGAA TGGTGTTTAT 
GTTTTTAATG TTCCACAAAG AGAGTGTATT GACCGTCCAA CAGCTAGGCT GATTAAAGAC 
TTTGATCTCA TTGAAGAATC ATTCATAAAA GATATTGGGA GTCAAAATGG TATAGTCTCA 
GATTCTCGGG AGAATTCTCT TTATAGTGCT CTTTGGGTTG CACAAGCACT ACTGCGCAAA 
GGATCTTCAA AGACAGCAGA TAAGCGCATA TTTCTGTTTA CCAATGAAGA TGATCCTTTT 
GGGAGTATGA GGATTTCTGT AAAAGAGGAT ATGACAAGGA CTACATTGCA GAGAGCTAAG 
GATGCACAAG ACCTTGGCAT CTCAATCGAG CTTCTTCCCC TCAGTCACCC TGATAAGCAA 
TTCGATATCT CTCTCTTCTA CAAGGATTTA ATTGGATTAA ATGGTGATGA GCTTACGGAG 
TTCATGCCAT CAGTTGGTCA GAAGTTAGAG GACATGAAGG ATCAACTGAA GAAGCGTGTA 
CTTGCCAAGA GAATAGCAAA ACGAATAACG TTTATGATAT GTGATGGTGT TTCTATTGAA 
CTGAATGGTT ATGCTTTACT CCGTCCTGCT ACTCCTGGAA CCATAACCTG GCTTGACTCT 
ACTACTAATC TTCCTATAAA GGTGGAAAGA TCATATGTTT GTGCGGACAC TGGTGCGATA 
ATGCAAGAAC CTATTCAACG GATCCAGCCT TACATGAATC GGGATGTCAT GTTTACAGCG 
GCAGAGCTAT CCGAACTAAA AAAGATATCA ACTGGACATT TAAGACTGCT GGGGTTCAAG 
CCTCTTAGTT GCCTCAAAGA TTATCACAAC ATGAAGCCTT CTACCTTTCT CTATCCAAGC 
GATAAGGAAG TTATTGGGAG CACTCGTGCA TTCATTGCCT TGCATAGATC CATGATACAG 
CTTGGACGTT TTGCTGTTGC CTTTTATGGT GGTACGACAC CTCCTAGATT GGTTGCACTT 
GTTGCTCAGG ATGAAATAGA GAGTGATGGT GGTCAAGTGG AGCCACCAGG GATGAATATG 
ATATACCTTC CATATGCTAA TGATATTAGA GATATCGAAG AGTTGCATTC AAAACCTGGT 
GTAGCTGCTC CTCGAGCATC AGAAGATCAG CTCAAAAAGG CATCAGCTCT AATGCGACGC 
CTAGAGTTAA AGGACTTTTC TGTTTGCCAA TTTGCTAATC CCGCTTTACA GAGACACTAC 
GCGATGTTGC AAGCCATTGC ATTGGATGAA GACCAGCTTG GAGAAACAAG AGATGAGACT 
CTTCCTGACG AAGGAGGAAT GAACAGACCT GGAGTTGTAA AAGCAGTAGA AGAATTTAAA 
GAGTCTATTT ACGGTGATGA CTCTGAGGAA GAAAGTGACT CAGGGGCAAA GGAAAAATCA 
AGAAAGCGAA AAGCTGCAGA TGCAGGAGAC TATGACTTTG TCGAGCTTGC AAAAACCGGG 
AAGCTTAAAG ACTTGACGGT TGTGGAGCTG AAGACATACT TGACTGCCAA CAATCTTCCC 
CTCAGCGGGA AGAAAGATGC TCTCATCAAT AGGATTCTGG CTCACGTCAG TAAATAA