PIEZO1 cDNA ORF clone, Bos taurus(cattle)

The following PIEZO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225442 XM_015475782.2
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225443 XM_015475783.2
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225444 XM_015475784.2
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225445 XM_015475785.2
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225446 XM_015475781.2
Latest version!
Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa93378 XM_015475781.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa93378 XM_015467307.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa93379 XM_015467308.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa93379 XM_015475782.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OBa93380 XM_015467309.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93380 XM_015475783.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93381 XM_015475784.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93381 XM_015467310.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93382 XM_015467311.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93382 XM_015475785.1
Latest version!
Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225442
    Clone ID Related Accession (Same CDS sequence) XM_015475782.2
    Accession Version XM_015475782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGGCGAGGAA
    GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG GCGGCGGGGG CAGCGACCTT GCGGAGAGAG
    AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC
    TGCCTGTCCC TGGCCCGGAG CATGTACTGG CCCGTGCGGC GCTTCTTCCA GGACATCCTG
    CACACGAAGT ACCGGGCCGC GACCGACGTC TACGCGCTCA TGTTCTTGGC CGACGTCATC
    GACTTCATCA TCATCATCTT CGGATTCTGG GCCTTTGGGA AGCACTCGGC AGCCACGGAC
    ATCACGTCCT CCTTGTCAGA TGACCAGGTG CCCGAGGCCT TCCTGGTCAT GCTGCTGATC
    CAGTTCAGCA CCATGGTCAT TGACCGCGCC CTCTACCTGC GCAAGACCGT GCTGGGCAAG
    CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC GTCCACCTCT GGATGTTCTT CATCCTGCCC
    GCTGTCACTG AGCGGATGTT CAGCCAGAAC GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA
    TGCATCTACT TCGCTCTGTC CGCCTACCAG ATCCGCTGTG GCTACCCCAC CCGCATCCTT
    GGCAACTTCC TCACCAAGAA GTACAACCAC CTCAACCTTT TCCTCTTCCA GGGGTTCCGG
    CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA GTGATGGACT GGGTGTGGAC GGACACCACG
    CTGTCCCTGT CCAACTGGAT GTGCGTGGAG GACATCTATG CCAACATCTT CATCATCAAG
    TGCAGCCGAG AGACTGAGAA GAAATACCCG CAGCCCAAGG GGCAGAAGAA GAAAAAGATT
    GTCAAGTACG GCATGGGCGG CCTCATCATC CTGTTCCTCG TGGCCATCAT CTGGTTCCCG
    CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG GTCGGCGTCG TCAACCAGCC CATCGACGTC
    ACCGTCACCC TCAAGCTGGG CGGCTATGAG GTGAGCATCA TAAGGGGGTC TGGCTGCACG
    TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT CCGGGCGCCA GGTCACCATG TCCCCACCAC
    CAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAGTGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331268.2
    CDS359..7990
    Translation

    Target ORF information:

    RefSeq Version XM_015475782.2
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGGCGAGGAA
    GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG GCGGCGGGGG CAGCGACCTT GCGGAGAGAG
    AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC
    TGCCTGTCCC TGGCCCGGAG CATGTACTGG CCCGTGCGGC GCTTCTTCCA GGACATCCTG
    CACACGAAGT ACCGGGCCGC GACCGACGTC TACGCGCTCA TGTTCTTGGC CGACGTCATC
    GACTTCATCA TCATCATCTT CGGATTCTGG GCCTTTGGGA AGCACTCGGC AGCCACGGAC
    ATCACGTCCT CCTTGTCAGA TGACCAGGTG CCCGAGGCCT TCCTGGTCAT GCTGCTGATC
    CAGTTCAGCA CCATGGTCAT TGACCGCGCC CTCTACCTGC GCAAGACCGT GCTGGGCAAG
    CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC GTCCACCTCT GGATGTTCTT CATCCTGCCC
    GCTGTCACTG AGCGGATGTT CAGCCAGAAC GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA
    TGCATCTACT TCGCTCTGTC CGCCTACCAG ATCCGCTGTG GCTACCCCAC CCGCATCCTT
    GGCAACTTCC TCACCAAGAA GTACAACCAC CTCAACCTTT TCCTCTTCCA GGGGTTCCGG
    CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA GTGATGGACT GGGTGTGGAC GGACACCACG
    CTGTCCCTGT CCAACTGGAT GTGCGTGGAG GACATCTATG CCAACATCTT CATCATCAAG
    TGCAGCCGAG AGACTGAGAA GAAATACCCG CAGCCCAAGG GGCAGAAGAA GAAAAAGATT
    GTCAAGTACG GCATGGGCGG CCTCATCATC CTGTTCCTCG TGGCCATCAT CTGGTTCCCG
    CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG GTCGGCGTCG TCAACCAGCC CATCGACGTC
    ACCGTCACCC TCAAGCTGGG CGGCTATGAG GTGAGCATCA TAAGGGGGTC TGGCTGCACG
    TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT CCGGGCGCCA GGTCACCATG TCCCCACCAC
    CAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAGTGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225443
    Clone ID Related Accession (Same CDS sequence) XM_015475783.2
    Accession Version XM_015475783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGT TATCCACTCT GGGGACTATT TCCTGTTCGA GTCGGACAGT
    GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC CCCAGGCCAT CGTCACAGAG CGCCTTTCAG
    ATGGCCTACC AGGCGTGGGT GACCAACGCG CAGACGGTGC TTCGGCAGCA GCGGCAGCAG
    AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC
    TTGGAGGACG AGGTGACCGG CCGCAGCCAT GTGATGCAGA GAGTCCTGAG CGCCGTGCAG
    TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG GACGGGCTGA CGCGCTGGCT GCACGACTTC
    ACGCGGCACC ACCGCGCCAT AAGCGACGTA CTGCGCGCAG AGCGCTACCT GCTCACGCAG
    GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG GACGTGCTGG ACCAGCTGTA CGCCAACGAA
    GCTGAGGCAG CCCGTGATGC GCTGAGCACA GCATCGAGCG GGCTGGGGGT GGAGGAGCCG
    CTGAGCAGCG TGACCGAGGA CACCAGCAGC CCCCTGAGCA CTGGCTACAA TACCCGCAGC
    AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC
    GCTGGCGGCC GCGCCCGAAT GCGCACGGCC AGCGAGCTGC TGGTGGACAG GCGCGTGCAC
    ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG
    CTGGAGGCCC TGTACCAGTG CGTGGCTGCC CACTCGGAGC TGCTGTGCTA CTTCGTGATC
    GTCCTCAACC ACATGGTCAC TGCCTCAGCT ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC
    CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC AGCAAGCGCT TCTGGATGAC GGCCATCATC
    TTCACGGAGG TCACAGTGGT CGCCAAGTAT CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    AGCCACGCTG TGCTGCGGCG CTATGAAAAC AAGCCCTACT TCCCGCCGCG CATCCTGGGC
    CTGGAGAAGA GCGACAGCTA CGTCAAGTAC GACCTGCTGC AGCTCATGGC GCTATTCTTC
    CACCGCGCAC AGCTGCTGTG CTACGGCCTC TGGGACCATG AGGACCCCCC ATTGTCCAAG
    GAGCACGATA GGGGCAGTGC AAAGGAGAAG GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG
    CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG CCCCCTGCTG AGGACAATGT CCAGGCGGAA
    GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG
    AGGAGGAAGG AGAGCACGGA AACCAGAGGA CAGGCCATTG AAGGCGAGGA AGAGGAGGAG
    GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG GCAGCGACCT TGCGGAGAGA GAAGAGGCGG
    AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC
    CTGGCCCGGA GCATGTACTG GCCCGTGCGG CGCTTCTTCC AGGACATCCT GCACACGAAG
    TACCGGGCCG CGACCGACGT CTACGCGCTC ATGTTCTTGG CCGACGTCAT CGACTTCATC
    ATCATCATCT TCGGATTCTG GGCCTTTGGG AAGCACTCGG CAGCCACGGA CATCACGTCC
    TCCTTGTCAG ATGACCAGGT GCCCGAGGCC TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC
    ACCATGGTCA TTGACCGCGC CCTCTACCTG CGCAAGACCG TGCTGGGCAA GCTGGCCTTC
    CAGGTCGTCC TGGTGCTGGC CGTCCACCTC TGGATGTTCT TCATCCTGCC CGCTGTCACT
    GAGCGGATGT TCAGCCAGAA CGCGGTGGCC CAGCTGTGGT ACTTTGTGAA ATGCATCTAC
    TTCGCTCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGCATCCT TGGCAACTTC
    CTCACCAAGA AGTACAACCA CCTCAACCTT TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA
    TTCCTGGTGG AGCTGCGGGC AGTGATGGAC TGGGTGTGGA CGGACACCAC GCTGTCCCTG
    TCCAACTGGA TGTGCGTGGA GGACATCTAT GCCAACATCT TCATCATCAA GTGCAGCCGA
    GAGACTGAGA AGAAATACCC GCAGCCCAAG GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC
    GGCATGGGCG GCCTCATCAT CCTGTTCCTC GTGGCCATCA TCTGGTTCCC GCTGCTCTTC
    ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC GTCAACCAGC CCATCGACGT CACCGTCACC
    CTCAAGCTGG GCGGCTATGA GGTGAGCATC ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG
    TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC AGGTCACCAT GTCCCCACCA CCAGCCCCTG
    TTCACCATGA GCGCCCAGCA GCCATCCATC GTGCCCTTCA CGCACCACGC CTACGAGGAG
    CTGTCCAAGC AGTTTGACCC CCACCCACTG GCCATGCAGT TCATCAGCCA GTACAGCCCT
    GAGGATATCG TCACAGTGCA CATCGAGGGC AGCTCCGGGG CACTGTGGCG CATCAGCCCG
    CCAAGCCGGG CACAGATGAA GCGGGAGCTG TATAACGGCA CGGCCGACAT CACCCTGCGC
    TTCACCTGGA ACTTCCAGAG GGACCTGGCC AAGGGCGGCA GCGTGGAGTA CACCAACGAG
    AAGCACACCC TGGACCTGGC CCCTAACAGC ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG
    GAAGGCACCT CGGACCAGTC CGTGGTCATC CCCCACCTCT TCCCCAAGTA CATCCGTGCC
    CCCAGTGGGC CTGAAGCCAA CCCCGTGAAG CAGCTGCAGC CCAACGAGGA GGCTGACTAT
    CTCGGCGTGC GTATCCAGCT GCGCAGGGAG CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC
    GAGTGGTGGG TCATTGAGCT GCAGGACTGC CAGGCCAACT GCAACCTACT GCCCATGGTC
    ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC CTGGGCTTCC TGGCCGGCTA TGGCATCATG
    GGGCTGTACG TGTCCATCGT GCTGGTCATC GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG
    ATCTCGCACT CCATCATGTT TGAGGAGCTG CCTTGCGTGG ACCGCATCCT CAAGCTATGC
    CAGGACATCT TCCTGGTGCG GGAGACGCGG GAGCTGGAGC TGGAGGAGGA GCTCTACGCC
    AAGCTCATCT TCCTGTACCG CTCACCCGAG ACCATGATCA AGTGGACGCG CGACAAGGAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331269.2
    CDS358..7920
    Translation

    Target ORF information:

    RefSeq Version XM_015475783.2
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGT TATCCACTCT GGGGACTATT TCCTGTTCGA GTCGGACAGT
    GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC CCCAGGCCAT CGTCACAGAG CGCCTTTCAG
    ATGGCCTACC AGGCGTGGGT GACCAACGCG CAGACGGTGC TTCGGCAGCA GCGGCAGCAG
    AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC
    TTGGAGGACG AGGTGACCGG CCGCAGCCAT GTGATGCAGA GAGTCCTGAG CGCCGTGCAG
    TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG GACGGGCTGA CGCGCTGGCT GCACGACTTC
    ACGCGGCACC ACCGCGCCAT AAGCGACGTA CTGCGCGCAG AGCGCTACCT GCTCACGCAG
    GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG GACGTGCTGG ACCAGCTGTA CGCCAACGAA
    GCTGAGGCAG CCCGTGATGC GCTGAGCACA GCATCGAGCG GGCTGGGGGT GGAGGAGCCG
    CTGAGCAGCG TGACCGAGGA CACCAGCAGC CCCCTGAGCA CTGGCTACAA TACCCGCAGC
    AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC
    GCTGGCGGCC GCGCCCGAAT GCGCACGGCC AGCGAGCTGC TGGTGGACAG GCGCGTGCAC
    ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG
    CTGGAGGCCC TGTACCAGTG CGTGGCTGCC CACTCGGAGC TGCTGTGCTA CTTCGTGATC
    GTCCTCAACC ACATGGTCAC TGCCTCAGCT ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC
    CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC AGCAAGCGCT TCTGGATGAC GGCCATCATC
    TTCACGGAGG TCACAGTGGT CGCCAAGTAT CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC
    AGCCACGCTG TGCTGCGGCG CTATGAAAAC AAGCCCTACT TCCCGCCGCG CATCCTGGGC
    CTGGAGAAGA GCGACAGCTA CGTCAAGTAC GACCTGCTGC AGCTCATGGC GCTATTCTTC
    CACCGCGCAC AGCTGCTGTG CTACGGCCTC TGGGACCATG AGGACCCCCC ATTGTCCAAG
    GAGCACGATA GGGGCAGTGC AAAGGAGAAG GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG
    CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG CCCCCTGCTG AGGACAATGT CCAGGCGGAA
    GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG
    AGGAGGAAGG AGAGCACGGA AACCAGAGGA CAGGCCATTG AAGGCGAGGA AGAGGAGGAG
    GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG GCAGCGACCT TGCGGAGAGA GAAGAGGCGG
    AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC
    CTGGCCCGGA GCATGTACTG GCCCGTGCGG CGCTTCTTCC AGGACATCCT GCACACGAAG
    TACCGGGCCG CGACCGACGT CTACGCGCTC ATGTTCTTGG CCGACGTCAT CGACTTCATC
    ATCATCATCT TCGGATTCTG GGCCTTTGGG AAGCACTCGG CAGCCACGGA CATCACGTCC
    TCCTTGTCAG ATGACCAGGT GCCCGAGGCC TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC
    ACCATGGTCA TTGACCGCGC CCTCTACCTG CGCAAGACCG TGCTGGGCAA GCTGGCCTTC
    CAGGTCGTCC TGGTGCTGGC CGTCCACCTC TGGATGTTCT TCATCCTGCC CGCTGTCACT
    GAGCGGATGT TCAGCCAGAA CGCGGTGGCC CAGCTGTGGT ACTTTGTGAA ATGCATCTAC
    TTCGCTCTGT CCGCCTACCA GATCCGCTGT GGCTACCCCA CCCGCATCCT TGGCAACTTC
    CTCACCAAGA AGTACAACCA CCTCAACCTT TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA
    TTCCTGGTGG AGCTGCGGGC AGTGATGGAC TGGGTGTGGA CGGACACCAC GCTGTCCCTG
    TCCAACTGGA TGTGCGTGGA GGACATCTAT GCCAACATCT TCATCATCAA GTGCAGCCGA
    GAGACTGAGA AGAAATACCC GCAGCCCAAG GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC
    GGCATGGGCG GCCTCATCAT CCTGTTCCTC GTGGCCATCA TCTGGTTCCC GCTGCTCTTC
    ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC GTCAACCAGC CCATCGACGT CACCGTCACC
    CTCAAGCTGG GCGGCTATGA GGTGAGCATC ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG
    TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC AGGTCACCAT GTCCCCACCA CCAGCCCCTG
    TTCACCATGA GCGCCCAGCA GCCATCCATC GTGCCCTTCA CGCACCACGC CTACGAGGAG
    CTGTCCAAGC AGTTTGACCC CCACCCACTG GCCATGCAGT TCATCAGCCA GTACAGCCCT
    GAGGATATCG TCACAGTGCA CATCGAGGGC AGCTCCGGGG CACTGTGGCG CATCAGCCCG
    CCAAGCCGGG CACAGATGAA GCGGGAGCTG TATAACGGCA CGGCCGACAT CACCCTGCGC
    TTCACCTGGA ACTTCCAGAG GGACCTGGCC AAGGGCGGCA GCGTGGAGTA CACCAACGAG
    AAGCACACCC TGGACCTGGC CCCTAACAGC ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG
    GAAGGCACCT CGGACCAGTC CGTGGTCATC CCCCACCTCT TCCCCAAGTA CATCCGTGCC
    CCCAGTGGGC CTGAAGCCAA CCCCGTGAAG CAGCTGCAGC CCAACGAGGA GGCTGACTAT
    CTCGGCGTGC GTATCCAGCT GCGCAGGGAG CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC
    GAGTGGTGGG TCATTGAGCT GCAGGACTGC CAGGCCAACT GCAACCTACT GCCCATGGTC
    ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC CTGGGCTTCC TGGCCGGCTA TGGCATCATG
    GGGCTGTACG TGTCCATCGT GCTGGTCATC GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG
    ATCTCGCACT CCATCATGTT TGAGGAGCTG CCTTGCGTGG ACCGCATCCT CAAGCTATGC
    CAGGACATCT TCCTGGTGCG GGAGACGCGG GAGCTGGAGC TGGAGGAGGA GCTCTACGCC
    AAGCTCATCT TCCTGTACCG CTCACCCGAG ACCATGATCA AGTGGACGCG CGACAAGGAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225444
    Clone ID Related Accession (Same CDS sequence) XM_015475784.2
    Accession Version XM_015475784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAGTGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331270.2
    CDS357..7898
    Translation

    Target ORF information:

    RefSeq Version XM_015475784.2
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAGTGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225445
    Clone ID Related Accession (Same CDS sequence) XM_015475785.2
    Accession Version XM_015475785.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGGTGAGCA TCATAAGGGG GTCTGGCTGC
    ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC TCTCCGGGCG CCAGGTCACC ATGTCCCCAC
    CACCAGCCCC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TCGTGCCCTT CACGCACCAC
    GCCTACGAGG AGCTGTCCAA GCAGTTTGAC CCCCACCCAC TGGCCATGCA GTTCATCAGC
    CAGTACAGCC CTGAGGATAT CGTCACAGTG CACATCGAGG GCAGCTCCGG GGCACTGTGG
    CGCATCAGCC CGCCAAGCCG GGCACAGATG AAGCGGGAGC TGTATAACGG CACGGCCGAC
    ATCACCCTGC GCTTCACCTG GAACTTCCAG AGGGACCTGG CCAAGGGCGG CAGCGTGGAG
    TACACCAACG AGAAGCACAC CCTGGACCTG GCCCCTAACA GCACTGCCCG GCGGCAGCTG
    GCCAGCCTGC TGGAAGGCAC CTCGGACCAG TCCGTGGTCA TCCCCCACCT CTTCCCCAAG
    TACATCCGTG CCCCCAGTGG GCCTGAAGCC AACCCCGTGA AGCAGCTGCA GCCCAACGAG
    GAGGCTGACT ATCTCGGCGT GCGTATCCAG CTGCGCAGGG AGCGTGTGGG CACAGGGGCA
    GCTGGCTTCC TCGAGTGGTG GGTCATTGAG CTGCAGGACT GCCAGGCCAA CTGCAACCTA
    CTGCCCATGG TCATCTTCAG TGACAAGCAT CATGGGGCTG TACGTGTCCA TCGTGCTGGT
    CATCGGCAAG TTCGTGCGCG GCTTCTTCAG CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331271.2
    CDS359..7795
    Translation

    Target ORF information:

    RefSeq Version XM_015475785.2
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475785.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGGTGAGCA TCATAAGGGG GTCTGGCTGC
    ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC TCTCCGGGCG CCAGGTCACC ATGTCCCCAC
    CACCAGCCCC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TCGTGCCCTT CACGCACCAC
    GCCTACGAGG AGCTGTCCAA GCAGTTTGAC CCCCACCCAC TGGCCATGCA GTTCATCAGC
    CAGTACAGCC CTGAGGATAT CGTCACAGTG CACATCGAGG GCAGCTCCGG GGCACTGTGG
    CGCATCAGCC CGCCAAGCCG GGCACAGATG AAGCGGGAGC TGTATAACGG CACGGCCGAC
    ATCACCCTGC GCTTCACCTG GAACTTCCAG AGGGACCTGG CCAAGGGCGG CAGCGTGGAG
    TACACCAACG AGAAGCACAC CCTGGACCTG GCCCCTAACA GCACTGCCCG GCGGCAGCTG
    GCCAGCCTGC TGGAAGGCAC CTCGGACCAG TCCGTGGTCA TCCCCCACCT CTTCCCCAAG
    TACATCCGTG CCCCCAGTGG GCCTGAAGCC AACCCCGTGA AGCAGCTGCA GCCCAACGAG
    GAGGCTGACT ATCTCGGCGT GCGTATCCAG CTGCGCAGGG AGCGTGTGGG CACAGGGGCA
    GCTGGCTTCC TCGAGTGGTG GGTCATTGAG CTGCAGGACT GCCAGGCCAA CTGCAACCTA
    CTGCCCATGG TCATCTTCAG TGACAAGCAT CATGGGGCTG TACGTGTCCA TCGTGCTGGT
    CATCGGCAAG TTCGTGCGCG GCTTCTTCAG CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225446
    Clone ID Related Accession (Same CDS sequence) XM_015475781.2
    Accession Version XM_015475781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGGTGAGCA TCATAAGGGG GTCTGGCTGC
    ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC TCTCCGGGCG CCAGGTCACC ATGTCCCCAC
    CACCAGCCCC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TCGTGCCCTT CACGCACCAC
    GCCTACGAGG AGCTGTCCAA GCAGTTTGAC CCCCACCCAC TGGCCATGCA GTTCATCAGC
    CAGTACAGCC CTGAGGATAT CGTCACAGTG CACATCGAGG GCAGCTCCGG GGCACTGTGG
    CGCATCAGCC CGCCAAGCCG GGCACAGATG AAGCGGGAGC TGTATAACGG CACGGCCGAC
    ATCACCCTGC GCTTCACCTG GAACTTCCAG AGGGACCTGG CCAAGGGCGG CAGCGTGGAG
    TACACCAACG AGAAGCACAC CCTGGACCTG GCCCCTAACA GCACTGCCCG GCGGCAGCTG
    GCCAGCCTGC TGGAAGGCAC CTCGGACCAG TCCGTGGTCA TCCCCCACCT CTTCCCCAAG
    TACATCCGTG CCCCCAGTGG GCCTGAAGCC AACCCCGTGA AGCAGCTGCA GCCCAACGAG
    GAGGCTGACT ATCTCGGCGT GCGTATCCAG CTGCGCAGGG AGCGTGTGGG CACAGGGGCA
    GCTGGCTTCC TCGAGTGGTG GGTCATTGAG CTGCAGGACT GCCAGGCCAA CTGCAACCTA
    CTGCCCATGG TCATCTTCAG TGACAAGGTC AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC
    TATGGCATCA TGGGGCTGTA CGTGTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC
    TTCTTCAGCG AGATCTCGCA CTCCATCATG TTTGAGGAGC TGCCTTGCGT GGACCGCATC
    CTCAAGCTAT GCCAGGACAT CTTCCTGGTG CGGGAGACGC GGGAGCTGGA GCTGGAGGAG
    GAGCTCTACG CCAAGCTCAT CTTCCTGTAC CGCTCACCCG AGACCATGAT CAAGTGGACG
    CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331267.2
    CDS393..8027
    Translation

    Target ORF information:

    RefSeq Version XM_015475781.2
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGCCGC ACGTGCTCGG CGCCGTCCTG TACTGGCTGC TGCTGCCCTT CGCGCTTCTG 
    GCCGCCTGCC TGTTCCGGGT CAACGCGCTC TCCCTGGTCT ACCTGCTGTT CCTGCTGCTG
    CTGCCCTGGT TCCCGGGCCC CTCCCGGCAC AGCATCCGAG GTCACACTGG TCGCCTCCTC
    CGAGCCCTGC TGGGCTTCAG CCTCCTCTTC TTGGCAGCCC ACATCACCTT CCAGATATGC
    CTGCATACTG TGCCCCGCCT GGACCAGCTT CTGGAGCCAA ACTGCAGCAC CGGGGACGCC
    ATCACCTGGG ATGCCATCTC CCAGCTCATT GGGGTCACGA GGCTGGACCT GAAGGACATT
    CCCAATGCCA TCCGCCTGGT GGCCCCTGAC CTGGGCATCC TAGTAGTCTC CTCCCTGTGC
    CTTGGGGTCT GCAGGCGCCT CACGAGGACA GCCCGGCGCA GCCAGCACGT CCAGGAGCCG
    GATGATGACA GTGAGGAACT GGACACTGGC TCACTGGGGG AGCTGCGGGA AGCCCCTGAA
    CTGTCCCCCA CGCGGAGATC TCGGCTGGCC GCCCGGTTCC GGATCACAGC TCACTGGCTG
    CTCGTGGCAG CTGGGCGGAC TCTGGCCATC ATGCTGCTCG CACTGGCAGG CATTGCCCAC
    CCCTCAGCCT TCTCCAGTGT CTACTTCCTG CTCTTCCTGG CCATTGGCAC TTGGTGGGCC
    TGTCACTTCC CCATCAGCCT GTTGGGCTTC AACACACTCT GCGTCATGGT GAGCTGCATT
    GGCACCGGCC ATCTCATCTG CCTCTATTGC TACCAGACAC CTCTCGCCCA GGCCACTCTC
    CCACCTGCTG GCATCTGGGC CAGGGTGTTT GGTCTCAAGG ACTTTTTGGC CCCCACCAAC
    TGCTCCAGCC CCAACGTGCT GGTCATCAAT GCTAACCACG ACTGGCCCGT GTACGTGAGC
    CCCGGCATCC TGCTGCTGCT CTGCTACACG GTGACCTCGC TCTTGAAGCT CCATGCCCAC
    CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC GGGGACGATG ACACACGGGA GGTGGAGTTG
    ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG GCCAGAGTTG TGGCCATCAA GGAGGAAGGG
    GCTGCCCAGC ACACAATGCC TACTCCCACA ACACCAGACC CCGAGGACAA CTGCATCATA
    CACGACCTCA CTGGCCACAG CGCCGTCCAG CAGCGAACCT CATGCCCCCG GCTGGCTGAG
    CCAAAGGAGA CGTCTCCGCT GCACGGCCTG GGCCACCTCA TCATGGACCA GAGCTACGTG
    TGTGCCCTCA TCGCCATGAT GGTGTGGAGC ATCACCTACC ACAGCTGGCT GACCTTCGTG
    CTGCTGCTCT GGGCCTGCCT CATCTGGACG GTACGCAGCC GCCACCAGCT GGCCATGCTC
    TGCTCGCCCT TCATCCTGCT CTATGGGCTG GCACTCTGCT GCCTGCGTTA CGTGTGGGCC
    ATGGACCTGC GGCCCGAGCT GCCCACCGCC CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG
    CTGGAGCACA CCCGCTACCC CTGCCTGGAC CTGGGTGCCA TGCTGCTGTG CACCCTGACC
    TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT
    GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC ACGGAGCCCA CGCGGACGCA GATGCTGCTG
    CGGAGCCTGG GGGAGCTGGT CAGGGGCATC TACGCCAAGT ACTGGATCTA CGTGTGCGCA
    GGCATGTTCA TCGTGGTCAG CTTTGCTGGC CGCCTGGTGG TCTACAAGAT CGTCTACATG
    TTGCTCTTCC TGCTCTGTCT CATCCTCTTC CAGGTCTACT ACAGCCTGTG GCGGAAGCTG
    CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC TACACCATGC TGGTGTTGGT GGCCGTCTAC
    ACCTTCCAGT TCCAGGACTT CCCGGCCTAC TGGCGCAACC TGACGGGCCT CACCGATGAG
    CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC AGCGTGTCCG AGCTCTTCTC CAGCATCCTG
    GTCCCCGGCT TCTTCCTGCT TGCCTGCATT CTGCAGCTGC ACTACTTCCA CCGGCCCTTC
    ATGCAGCTCA CCGACCCCGA GCACCGGCCC CTACCTGGTG CCTGCACCCC ACGCTGGGTT
    CCCGGGCGGG ACGCGGTGAG CCGGACCCCC CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA
    CCCAGGGACG AGGGGCTGGG CACGGCCAGC CCCCACCAGG TCACACAGGT CCCAGAGGCC
    AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG CTGGACCTGG CCACCGGCTT CTCGGACGTC
    ATCACCCGCG TGCAGGTGCT GGTGCGGCGC CTGCTGGAGC TGCACGTCTT CAAGCTGGTG
    GCCCTGTACA CCGTGTGGGT GGCCCTGAAG GAGGTGTCGG TGCTGAACTT CCTGCTGGTC
    GTGCTCTGGG CCTTCGCCCT GCCCTACCCA CGCTTCCGGC CCATGGCCTC ATGCCTGGCC
    ACTGTGTGGA CCTGCATCAT CATCGTGTGC AAGATGCTGT ACCAGCTCAA GGTGGTCAAC
    CCCCACGAGT ACGCCAGCAA CTGCACGGAG CCCTTCCCCA ATAGTACGAA CCTGCAGAAG
    ACGGAGATCC GCCAGTCCTT GCTGTATCGC GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG
    GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC ATCCAGAACC ACCTGCAGAT CCTGCTGCTG
    CTGGTGTTTG AGGCCATGGT GTACCGGAGC CAGGACTACC ACCACCGCCG GCACCAGCTG
    GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC GAGGGCACCC GCCAGCGGCT GGACCGCGAC
    CTGCCCAGCT GCCTCAAGTA CTACGTGAAC TTCTTCTTCT ACAAATTTGG GCTGGAGGTC
    TGCTTCCTGG CAGCCGTGAA CGTGATCGGG CAGCGCATGA ACTTCATGGT CATCCTGCAC
    GGCTGCTGGC TGGTGGCCAT CCTTACCCGC CGACGCCGGG AGGCCATCGC CCGCCTCTGG
    CCCAATTACT GCCTGTTCCT GGCGCTCTTC CTGCTGTACC AGTACCTGCT GTGCCTGGGT
    GTTCCCCCCG CCCTGTGCAT TGACTACCCA TGGCGCTGGA GCCGGGCCGT CCCCATGAAC
    TCCGCGCTCA TCAAGTGGCT ATACCTGCCT GATTTCTTCA GCACCCCCAA TGCCACCAAC
    CTCATCAGTG ACTTCCTGCT GCTGCTCTGC GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC
    GAGCGCATGG AGGAGTGGCG GCACATGGCT GGCGTCAATA CTGACCGCCT GGAGCCTCTG
    CGGGGGGAGC CCAACCCCGT GCCCAACTTC ATCCACTGCA GGTCCTACCT CGACATGCTG
    AAGGTGGCTG TCTTCCGCTA CTTCTTCTGG CTGGTGCTGG TGGTGGTGTT CATCACGGGG
    GCCACGCGCA TCAGCGTCTT TGGGCTGGGC TACCTACTGG CCTGCTTCTA CTTGCTGCTC
    TTCGGCACCA GCCTGCAGCA GAAGGACACG CGTGCCCGCC TCGTGCTGTG GGACTGCCTC
    ATCCTTTACA ATGTCACTGT CATCGTCTCC AAGAACATGC TCTCTCTCCT GTCCTGCGTC
    TTCGTGGAGC AGATGCAGAG CAGCTTCTGC TGGGTCATCC AGCTCTTCAG CCTCGTATGC
    ACCGTCAAGG GCTACTACGA CCCCAAGGAG ATGCTGGGCC GCGACCAGGA CTGCCTGCTG
    CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG
    CGCAGGGTGT TCCTCAGCTA CTACTTCCTG CACGTTCAGG CCGAGCTCCG CGCCACCGCC
    CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC AATGCTGCCA ATCTCAAGAC CATCGAGCTC
    CACCGCAGAG CGGAGGAGAA GTCACTGGCC CAGCTGAAAA GACAGATGGA GCGGATCCGG
    GCCAAGCAAG AGAAGCACAG GCAGGGCCGG GCCAACCGAG GCTGCCTTCA GGGCTCCCCG
    GATCCCGGCC AAGAGCCAGG GCCCAGCAGC CCAGGGGGCT CCTCCCCGCC ACAACAACAG
    TGGTGGCGGC CCTGGCTGGA CCACGCCACA GTTATCCACT CTGGGGACTA TTTCCTGTTC
    GAGTCGGACA GTGAGGAGGA GGAGGAAGCC CAGCCCGAGG ACCCCAGGCC ATCGTCACAG
    AGCGCCTTTC AGATGGCCTA CCAGGCGTGG GTGACCAACG CGCAGACGGT GCTTCGGCAG
    CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG
    CCGGCAGAGG GCTTGGAGGA CGAGGTGACC GGCCGCAGCC ATGTGATGCA GAGAGTCCTG
    AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC CAGGCGCTGG TGGACGGGCT GACGCGCTGG
    CTGCACGACT TCACGCGGCA CCACCGCGCC ATAAGCGACG TACTGCGCGC AGAGCGCTAC
    CTGCTCACGC AGGAGCTGCT CCGGGGCGGA GAGGTGCGCC GGGACGTGCT GGACCAGCTG
    TACGCCAACG AAGCTGAGGC AGCCCGTGAT GCGCTGAGCA CAGCATCGAG CGGGCTGGGG
    GTGGAGGAGC CGCTGAGCAG CGTGACCGAG GACACCAGCA GCCCCCTGAG CACTGGCTAC
    AATACCCGCA GCAGCAGCGA AGAGGTGGTC ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT
    GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA ATGCGCACGG CCAGCGAGCT GCTGGTGGAC
    AGGCGCGTGC ACATTCCGGA GCTGGAGGAG GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG
    GCGCTTCGGC TGCTGGAGGC CCTGTACCAG TGCGTGGCTG CCCACTCGGA GCTGCTGTGC
    TACTTCGTGA TCGTCCTCAA CCACATGGTC ACTGCCTCAG CTACCTCCCT TGTGCTGCCC
    GTGCTGGTCT TCCTGTGGGC CATGCTCTCC ATCCCGCGGC CCAGCAAGCG CTTCTGGATG
    ACGGCCATCA TCTTCACGGA GGTCACAGTG GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC
    TTCCCCTGGA ACAGCCACGC TGTGCTGCGG CGCTATGAAA ACAAGCCCTA CTTCCCGCCG
    CGCATCCTGG GCCTGGAGAA GAGCGACAGC TACGTCAAGT ACGACCTGCT GCAGCTCATG
    GCGCTATTCT TCCACCGCGC ACAGCTGCTG TGCTACGGCC TCTGGGACCA TGAGGACCCC
    CCATTGTCCA AGGAGCACGA TAGGGGCAGT GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA
    GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA GGGGCGGCCA GGCCCCCTGC TGAGGACAAT
    GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA GAGCCCGGCG GCAAGAGGCG CATCAGCCTC
    CGTTTCAGGA GGAGGAGGAA GGAGAGCACG GAAACCAGAG GACAGGCCAT TGAAGGCGAG
    GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA
    GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC
    TTCTGCCTGT CCCTGGCCCG GAGCATGTAC TGGCCCGTGC GGCGCTTCTT CCAGGACATC
    CTGCACACGA AGTACCGGGC CGCGACCGAC GTCTACGCGC TCATGTTCTT GGCCGACGTC
    ATCGACTTCA TCATCATCAT CTTCGGATTC TGGGCCTTTG GGAAGCACTC GGCAGCCACG
    GACATCACGT CCTCCTTGTC AGATGACCAG GTGCCCGAGG CCTTCCTGGT CATGCTGCTG
    ATCCAGTTCA GCACCATGGT CATTGACCGC GCCCTCTACC TGCGCAAGAC CGTGCTGGGC
    AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG GCCGTCCACC TCTGGATGTT CTTCATCCTG
    CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG AACGCGGTGG CCCAGCTGTG GTACTTTGTG
    AAATGCATCT ACTTCGCTCT GTCCGCCTAC CAGATCCGCT GTGGCTACCC CACCCGCATC
    CTTGGCAACT TCCTCACCAA GAAGTACAAC CACCTCAACC TTTTCCTCTT CCAGGGGTTC
    CGGCTGGTGC CATTCCTGGT GGAGCTGCGG GCAGTGATGG ACTGGGTGTG GACGGACACC
    ACGCTGTCCC TGTCCAACTG GATGTGCGTG GAGGACATCT ATGCCAACAT CTTCATCATC
    AAGTGCAGCC GAGAGACTGA GAAGAAATAC CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG
    ATTGTCAAGT ACGGCATGGG CGGCCTCATC ATCCTGTTCC TCGTGGCCAT CATCTGGTTC
    CCGCTGCTCT TCATGTCCCT GGTGCGCTCT GTGGTCGGCG TCGTCAACCA GCCCATCGAC
    GTCACCGTCA CCCTCAAGCT GGGCGGCTAT GAGGTGAGCA TCATAAGGGG GTCTGGCTGC
    ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC TCTCCGGGCG CCAGGTCACC ATGTCCCCAC
    CACCAGCCCC TGTTCACCAT GAGCGCCCAG CAGCCATCCA TCGTGCCCTT CACGCACCAC
    GCCTACGAGG AGCTGTCCAA GCAGTTTGAC CCCCACCCAC TGGCCATGCA GTTCATCAGC
    CAGTACAGCC CTGAGGATAT CGTCACAGTG CACATCGAGG GCAGCTCCGG GGCACTGTGG
    CGCATCAGCC CGCCAAGCCG GGCACAGATG AAGCGGGAGC TGTATAACGG CACGGCCGAC
    ATCACCCTGC GCTTCACCTG GAACTTCCAG AGGGACCTGG CCAAGGGCGG CAGCGTGGAG
    TACACCAACG AGAAGCACAC CCTGGACCTG GCCCCTAACA GCACTGCCCG GCGGCAGCTG
    GCCAGCCTGC TGGAAGGCAC CTCGGACCAG TCCGTGGTCA TCCCCCACCT CTTCCCCAAG
    TACATCCGTG CCCCCAGTGG GCCTGAAGCC AACCCCGTGA AGCAGCTGCA GCCCAACGAG
    GAGGCTGACT ATCTCGGCGT GCGTATCCAG CTGCGCAGGG AGCGTGTGGG CACAGGGGCA
    GCTGGCTTCC TCGAGTGGTG GGTCATTGAG CTGCAGGACT GCCAGGCCAA CTGCAACCTA
    CTGCCCATGG TCATCTTCAG TGACAAGGTC AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC
    TATGGCATCA TGGGGCTGTA CGTGTCCATC GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC
    TTCTTCAGCG AGATCTCGCA CTCCATCATG TTTGAGGAGC TGCCTTGCGT GGACCGCATC
    CTCAAGCTAT GCCAGGACAT CTTCCTGGTG CGGGAGACGC GGGAGCTGGA GCTGGAGGAG
    GAGCTCTACG CCAAGCTCAT CTTCCTGTAC CGCTCACCCG AGACCATGAT CAAGTGGACG
    CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93378
    Clone ID Related Accession (Same CDS sequence) XM_015467307.1 , XM_015475781.1
    Accession Version XM_015467307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC TATGGCATCA TGGGGCTGTA CGTGTCCATC
    GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC TTCTTCAGCG AGATCTCGCA CTCCATCATG
    TTTGAGGAGC TGCCTTGCGT GGACCGCATC CTCAAGCTAT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAGGAG GAGCTCTACG CCAAGCTCAT CTTCCTGTAC
    CGCTCACCCG AGACCATGAT CAAGTGGACG CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322793.1
    CDS174..7178
    Translation

    Target ORF information:

    RefSeq Version XM_015467307.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC TATGGCATCA TGGGGCTGTA CGTGTCCATC
    GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC TTCTTCAGCG AGATCTCGCA CTCCATCATG
    TTTGAGGAGC TGCCTTGCGT GGACCGCATC CTCAAGCTAT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAGGAG GAGCTCTACG CCAAGCTCAT CTTCCTGTAC
    CGCTCACCCG AGACCATGAT CAAGTGGACG CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93378
    Clone ID Related Accession (Same CDS sequence) XM_015467307.1 , XM_015475781.1
    Accession Version XM_015475781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC TATGGCATCA TGGGGCTGTA CGTGTCCATC
    GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC TTCTTCAGCG AGATCTCGCA CTCCATCATG
    TTTGAGGAGC TGCCTTGCGT GGACCGCATC CTCAAGCTAT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAGGAG GAGCTCTACG CCAAGCTCAT CTTCCTGTAC
    CGCTCACCCG AGACCATGAT CAAGTGGACG CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331267.1
    CDS167..7171
    Translation

    Target ORF information:

    RefSeq Version XM_015475781.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGGTC
    AGCCCGCCCA GCCTGGGCTT CCTGGCCGGC TATGGCATCA TGGGGCTGTA CGTGTCCATC
    GTGCTGGTCA TCGGCAAGTT CGTGCGCGGC TTCTTCAGCG AGATCTCGCA CTCCATCATG
    TTTGAGGAGC TGCCTTGCGT GGACCGCATC CTCAAGCTAT GCCAGGACAT CTTCCTGGTG
    CGGGAGACGC GGGAGCTGGA GCTGGAGGAG GAGCTCTACG CCAAGCTCAT CTTCCTGTAC
    CGCTCACCCG AGACCATGAT CAAGTGGACG CGCGACAAGG AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93379
    Clone ID Related Accession (Same CDS sequence) XM_015475782.1 , XM_015467308.1
    Accession Version XM_015475782.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGGCGAGGAA GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG
    GCGGCGGGGG CAGCGACCTT GCGGAGAGAG AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG
    AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC TGCCTGTCCC TGGCCCGGAG CATGTACTGG
    CCCGTGCGGC GCTTCTTCCA GGACATCCTG CACACGAAGT ACCGGGCCGC GACCGACGTC
    TACGCGCTCA TGTTCTTGGC CGACGTCATC GACTTCATCA TCATCATCTT CGGATTCTGG
    GCCTTTGGGA AGCACTCGGC AGCCACGGAC ATCACGTCCT CCTTGTCAGA TGACCAGGTG
    CCCGAGGCCT TCCTGGTCAT GCTGCTGATC CAGTTCAGCA CCATGGTCAT TGACCGCGCC
    CTCTACCTGC GCAAGACCGT GCTGGGCAAG CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC
    GTCCACCTCT GGATGTTCTT CATCCTGCCC GCTGTCACTG AGCGGATGTT CAGCCAGAAC
    GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA TGCATCTACT TCGCTCTGTC CGCCTACCAG
    ATCCGCTGTG GCTACCCCAC CCGCATCCTT GGCAACTTCC TCACCAAGAA GTACAACCAC
    CTCAACCTTT TCCTCTTCCA GGGGTTCCGG CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA
    GTGATGGACT GGGTGTGGAC GGACACCACG CTGTCCCTGT CCAACTGGAT GTGCGTGGAG
    GACATCTACG CCAACATCTT CATCATCAAG TGCAGCCGAG AGACTGAGAA GAAATACCCG
    CAGCCCAAGG GGCAGAAGAA GAAAAAGATT GTCAAGTACG GCATGGGCGG CCTCATCATC
    CTGTTCCTCG TGGCCATCAT CTGGTTCCCG CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG
    GTCGGCGTCG TCAACCAGCC CATCGACGTC ACCGTCACCC TCAAGCTGGG CGGCTATGAG
    GTGAGCATCA TAAGGGGGTC TGGCTGCACG TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT
    CCGGGCGCCA GGTCACCATG TCCCCACCAC CAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAATGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331268.1
    CDS168..7169
    Translation

    Target ORF information:

    RefSeq Version XM_015475782.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGGCGAGGAA GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG
    GCGGCGGGGG CAGCGACCTT GCGGAGAGAG AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG
    AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC TGCCTGTCCC TGGCCCGGAG CATGTACTGG
    CCCGTGCGGC GCTTCTTCCA GGACATCCTG CACACGAAGT ACCGGGCCGC GACCGACGTC
    TACGCGCTCA TGTTCTTGGC CGACGTCATC GACTTCATCA TCATCATCTT CGGATTCTGG
    GCCTTTGGGA AGCACTCGGC AGCCACGGAC ATCACGTCCT CCTTGTCAGA TGACCAGGTG
    CCCGAGGCCT TCCTGGTCAT GCTGCTGATC CAGTTCAGCA CCATGGTCAT TGACCGCGCC
    CTCTACCTGC GCAAGACCGT GCTGGGCAAG CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC
    GTCCACCTCT GGATGTTCTT CATCCTGCCC GCTGTCACTG AGCGGATGTT CAGCCAGAAC
    GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA TGCATCTACT TCGCTCTGTC CGCCTACCAG
    ATCCGCTGTG GCTACCCCAC CCGCATCCTT GGCAACTTCC TCACCAAGAA GTACAACCAC
    CTCAACCTTT TCCTCTTCCA GGGGTTCCGG CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA
    GTGATGGACT GGGTGTGGAC GGACACCACG CTGTCCCTGT CCAACTGGAT GTGCGTGGAG
    GACATCTACG CCAACATCTT CATCATCAAG TGCAGCCGAG AGACTGAGAA GAAATACCCG
    CAGCCCAAGG GGCAGAAGAA GAAAAAGATT GTCAAGTACG GCATGGGCGG CCTCATCATC
    CTGTTCCTCG TGGCCATCAT CTGGTTCCCG CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG
    GTCGGCGTCG TCAACCAGCC CATCGACGTC ACCGTCACCC TCAAGCTGGG CGGCTATGAG
    GTGAGCATCA TAAGGGGGTC TGGCTGCACG TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT
    CCGGGCGCCA GGTCACCATG TCCCCACCAC CAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAATGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93379
    Clone ID Related Accession (Same CDS sequence) XM_015475782.1 , XM_015467308.1
    Accession Version XM_015467308.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGGCGAGGAA GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG
    GCGGCGGGGG CAGCGACCTT GCGGAGAGAG AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG
    AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC TGCCTGTCCC TGGCCCGGAG CATGTACTGG
    CCCGTGCGGC GCTTCTTCCA GGACATCCTG CACACGAAGT ACCGGGCCGC GACCGACGTC
    TACGCGCTCA TGTTCTTGGC CGACGTCATC GACTTCATCA TCATCATCTT CGGATTCTGG
    GCCTTTGGGA AGCACTCGGC AGCCACGGAC ATCACGTCCT CCTTGTCAGA TGACCAGGTG
    CCCGAGGCCT TCCTGGTCAT GCTGCTGATC CAGTTCAGCA CCATGGTCAT TGACCGCGCC
    CTCTACCTGC GCAAGACCGT GCTGGGCAAG CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC
    GTCCACCTCT GGATGTTCTT CATCCTGCCC GCTGTCACTG AGCGGATGTT CAGCCAGAAC
    GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA TGCATCTACT TCGCTCTGTC CGCCTACCAG
    ATCCGCTGTG GCTACCCCAC CCGCATCCTT GGCAACTTCC TCACCAAGAA GTACAACCAC
    CTCAACCTTT TCCTCTTCCA GGGGTTCCGG CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA
    GTGATGGACT GGGTGTGGAC GGACACCACG CTGTCCCTGT CCAACTGGAT GTGCGTGGAG
    GACATCTACG CCAACATCTT CATCATCAAG TGCAGCCGAG AGACTGAGAA GAAATACCCG
    CAGCCCAAGG GGCAGAAGAA GAAAAAGATT GTCAAGTACG GCATGGGCGG CCTCATCATC
    CTGTTCCTCG TGGCCATCAT CTGGTTCCCG CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG
    GTCGGCGTCG TCAACCAGCC CATCGACGTC ACCGTCACCC TCAAGCTGGG CGGCTATGAG
    GTGAGCATCA TAAGGGGGTC TGGCTGCACG TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT
    CCGGGCGCCA GGTCACCATG TCCCCACCAC CAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAATGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322794.1
    CDS174..7175
    Translation

    Target ORF information:

    RefSeq Version XM_015467308.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467308.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGGCGAGGAA GAGGAGGAGG AGGCAGCAGT GGCGGTGGTG
    GCGGCGGGGG CAGCGACCTT GCGGAGAGAG AAGAGGCGGA GTCGCCCCCA GGAAAGAGTG
    AGGGTGCTGG GGGTCCGGCT GCAGAGCTTC TGCCTGTCCC TGGCCCGGAG CATGTACTGG
    CCCGTGCGGC GCTTCTTCCA GGACATCCTG CACACGAAGT ACCGGGCCGC GACCGACGTC
    TACGCGCTCA TGTTCTTGGC CGACGTCATC GACTTCATCA TCATCATCTT CGGATTCTGG
    GCCTTTGGGA AGCACTCGGC AGCCACGGAC ATCACGTCCT CCTTGTCAGA TGACCAGGTG
    CCCGAGGCCT TCCTGGTCAT GCTGCTGATC CAGTTCAGCA CCATGGTCAT TGACCGCGCC
    CTCTACCTGC GCAAGACCGT GCTGGGCAAG CTGGCCTTCC AGGTCGTCCT GGTGCTGGCC
    GTCCACCTCT GGATGTTCTT CATCCTGCCC GCTGTCACTG AGCGGATGTT CAGCCAGAAC
    GCGGTGGCCC AGCTGTGGTA CTTTGTGAAA TGCATCTACT TCGCTCTGTC CGCCTACCAG
    ATCCGCTGTG GCTACCCCAC CCGCATCCTT GGCAACTTCC TCACCAAGAA GTACAACCAC
    CTCAACCTTT TCCTCTTCCA GGGGTTCCGG CTGGTGCCAT TCCTGGTGGA GCTGCGGGCA
    GTGATGGACT GGGTGTGGAC GGACACCACG CTGTCCCTGT CCAACTGGAT GTGCGTGGAG
    GACATCTACG CCAACATCTT CATCATCAAG TGCAGCCGAG AGACTGAGAA GAAATACCCG
    CAGCCCAAGG GGCAGAAGAA GAAAAAGATT GTCAAGTACG GCATGGGCGG CCTCATCATC
    CTGTTCCTCG TGGCCATCAT CTGGTTCCCG CTGCTCTTCA TGTCCCTGGT GCGCTCTGTG
    GTCGGCGTCG TCAACCAGCC CATCGACGTC ACCGTCACCC TCAAGCTGGG CGGCTATGAG
    GTGAGCATCA TAAGGGGGTC TGGCTGCACG TTGCTGCTGT GGCGGGGCTG GGGGGCCTCT
    CCGGGCGCCA GGTCACCATG TCCCCACCAC CAGCCCCTGT TCACCATGAG CGCCCAGCAG
    CCATCCATCG TGCCCTTCAC GCACCACGCC TACGAGGAGC TGTCCAAGCA GTTTGACCCC
    CACCCACTGG CCATGCAGTT CATCAGCCAG TACAGCCCTG AGGATATCGT CACAGTGCAC
    ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC ATCAGCCCGC CAAGCCGGGC ACAGATGAAG
    CGGGAGCTGT ATAACGGCAC GGCCGACATC ACCCTGCGCT TCACCTGGAA CTTCCAGAGG
    GACCTGGCCA AGGGCGGCAG CGTGGAGTAC ACCAACGAGA AGCACACCCT GGACCTGGCC
    CCTAACAGCA CTGCCCGGCG GCAGCTGGCC AGCCTGCTGG AAGGCACCTC GGACCAGTCC
    GTGGTCATCC CCCACCTCTT CCCCAAGTAC ATCCGTGCCC CCAATGGGCC TGAAGCCAAC
    CCCGTGAAGC AGCTGCAGCC CAACGAGGAG GCTGACTATC TCGGCGTGCG TATCCAGCTG
    CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT GGCTTCCTCG AGTGGTGGGT CATTGAGCTG
    CAGGACTGCC AGGCCAACTG CAACCTACTG CCCATGGTCA TCTTCAGTGA CAAGGTCAGC
    CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT GGCATCATGG GGCTGTACGT GTCCATCGTG
    CTGGTCATCG GCAAGTTCGT GCGCGGCTTC TTCAGCGAGA TCTCGCACTC CATCATGTTT
    GAGGAGCTGC CTTGCGTGGA CCGCATCCTC AAGCTATGCC AGGACATCTT CCTGGTGCGG
    GAGACGCGGG AGCTGGAGCT GGAGGAGGAG CTCTACGCCA AGCTCATCTT CCTGTACCGC
    TCACCCGAGA CCATGATCAA GTGGACGCGC GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93380
    Clone ID Related Accession (Same CDS sequence) XM_015467309.1 , XM_015475783.1
    Accession Version XM_015467309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGT TATCCACTCT
    GGGGACTATT TCCTGTTCGA GTCGGACAGT GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC
    CCCAGGCCAT CGTCACAGAG CGCCTTTCAG ATGGCCTACC AGGCGTGGGT GACCAACGCG
    CAGACGGTGC TTCGGCAGCA GCGGCAGCAG AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC
    CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC TTGGAGGACG AGGTGACCGG CCGCAGCCAT
    GTGATGCAGA GAGTCCTGAG CGCCGTGCAG TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG
    GACGGGCTGA CGCGCTGGCT GCACGACTTC ACGCGGCACC ACCGCGCCAT AAGCGACGTA
    CTGCGCGCAG AGCGCTACCT GCTCACGCAG GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG
    GACGTGCTGG ACCAGCTGTA CGCCAACGAA GCTGAGGCAG CCCGTGATGC GCTGAGCACA
    GCATCGAGCG GGCTGGGGGT GGAGGAGCCG CTGAGCAGCG TGACCGAGGA CACCAGCAGC
    CCCCTGAGCA CTGGCTACAA TACCCGCAGC AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG
    GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC GCTGGCGGCC GCGCCCGAAT GCGCACGGCC
    AGCGAGCTGC TGGTGGACAG GCGCGTGCAC ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC
    GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG CTGGAGGCCC TGTACCAGTG CGTGGCTGCC
    CACTCGGAGC TGCTGTGCTA CTTCGTGATC GTCCTCAACC ACATGGTCAC TGCCTCAGCT
    ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC
    AGCAAGCGCT TCTGGATGAC GGCCATCATC TTCACGGAGG TCACAGTGGT CGCCAAGTAT
    CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGCCACGCTG TGCTGCGGCG CTATGAAAAC
    AAGCCCTACT TCCCGCCGCG CATCCTGGGC CTGGAGAAGA GCGACAGCTA CGTCAAGTAC
    GACCTGCTGC AGCTCATGGC GCTATTCTTC CACCGCGCAC AGCTGCTGTG CTACGGCCTC
    TGGGACCATG AGGACCCCCC ATTGTCCAAG GAGCACGATA GGGGCAGTGC AAAGGAGAAG
    GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG
    CCCCCTGCTG AGGACAATGT CCAGGCGGAA GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC
    AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG AGGAGGAAGG AGAGCACGGA AACCAGAGGA
    CAGGCCATTG AAGGCGAGGA AGAGGAGGAG GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG
    GCAGCGACCT TGCGGAGAGA GAAGAGGCGG AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG
    GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC CTGGCCCGGA GCATGTACTG GCCCGTGCGG
    CGCTTCTTCC AGGACATCCT GCACACGAAG TACCGGGCCG CGACCGACGT CTACGCGCTC
    ATGTTCTTGG CCGACGTCAT CGACTTCATC ATCATCATCT TCGGATTCTG GGCCTTTGGG
    AAGCACTCGG CAGCCACGGA CATCACGTCC TCCTTGTCAG ATGACCAGGT GCCCGAGGCC
    TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC ACCATGGTCA TTGACCGCGC CCTCTACCTG
    CGCAAGACCG TGCTGGGCAA GCTGGCCTTC CAGGTCGTCC TGGTGCTGGC CGTCCACCTC
    TGGATGTTCT TCATCCTGCC CGCTGTCACT GAGCGGATGT TCAGCCAGAA CGCGGTGGCC
    CAGCTGTGGT ACTTTGTGAA ATGCATCTAC TTCGCTCTGT CCGCCTACCA GATCCGCTGT
    GGCTACCCCA CCCGCATCCT TGGCAACTTC CTCACCAAGA AGTACAACCA CCTCAACCTT
    TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA TTCCTGGTGG AGCTGCGGGC AGTGATGGAC
    TGGGTGTGGA CGGACACCAC GCTGTCCCTG TCCAACTGGA TGTGCGTGGA GGACATCTAC
    GCCAACATCT TCATCATCAA GTGCAGCCGA GAGACTGAGA AGAAATACCC GCAGCCCAAG
    GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC GGCATGGGCG GCCTCATCAT CCTGTTCCTC
    GTGGCCATCA TCTGGTTCCC GCTGCTCTTC ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC
    GTCAACCAGC CCATCGACGT CACCGTCACC CTCAAGCTGG GCGGCTATGA GGTGAGCATC
    ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC
    AGGTCACCAT GTCCCCACCA CCAGCCCCTG TTCACCATGA GCGCCCAGCA GCCATCCATC
    GTGCCCTTCA CGCACCACGC CTACGAGGAG CTGTCCAAGC AGTTTGACCC CCACCCACTG
    GCCATGCAGT TCATCAGCCA GTACAGCCCT GAGGATATCG TCACAGTGCA CATCGAGGGC
    AGCTCCGGGG CACTGTGGCG CATCAGCCCG CCAAGCCGGG CACAGATGAA GCGGGAGCTG
    TATAACGGCA CGGCCGACAT CACCCTGCGC TTCACCTGGA ACTTCCAGAG GGACCTGGCC
    AAGGGCGGCA GCGTGGAGTA CACCAACGAG AAGCACACCC TGGACCTGGC CCCTAACAGC
    ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG GAAGGCACCT CGGACCAGTC CGTGGTCATC
    CCCCACCTCT TCCCCAAGTA CATCCGTGCC CCCAATGGGC CTGAAGCCAA CCCCGTGAAG
    CAGCTGCAGC CCAACGAGGA GGCTGACTAT CTCGGCGTGC GTATCCAGCT GCGCAGGGAG
    CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC GAGTGGTGGG TCATTGAGCT GCAGGACTGC
    CAGGCCAACT GCAACCTACT GCCCATGGTC ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC
    CTGGGCTTCC TGGCCGGCTA TGGCATCATG GGGCTGTACG TGTCCATCGT GCTGGTCATC
    GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG ATCTCGCACT CCATCATGTT TGAGGAGCTG
    CCTTGCGTGG ACCGCATCCT CAAGCTATGC CAGGACATCT TCCTGGTGCG GGAGACGCGG
    GAGCTGGAGC TGGAGGAGGA GCTCTACGCC AAGCTCATCT TCCTGTACCG CTCACCCGAG
    ACCATGATCA AGTGGACGCG CGACAAGGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322795.1
    CDS174..7106
    Translation

    Target ORF information:

    RefSeq Version XM_015467309.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGT TATCCACTCT
    GGGGACTATT TCCTGTTCGA GTCGGACAGT GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC
    CCCAGGCCAT CGTCACAGAG CGCCTTTCAG ATGGCCTACC AGGCGTGGGT GACCAACGCG
    CAGACGGTGC TTCGGCAGCA GCGGCAGCAG AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC
    CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC TTGGAGGACG AGGTGACCGG CCGCAGCCAT
    GTGATGCAGA GAGTCCTGAG CGCCGTGCAG TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG
    GACGGGCTGA CGCGCTGGCT GCACGACTTC ACGCGGCACC ACCGCGCCAT AAGCGACGTA
    CTGCGCGCAG AGCGCTACCT GCTCACGCAG GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG
    GACGTGCTGG ACCAGCTGTA CGCCAACGAA GCTGAGGCAG CCCGTGATGC GCTGAGCACA
    GCATCGAGCG GGCTGGGGGT GGAGGAGCCG CTGAGCAGCG TGACCGAGGA CACCAGCAGC
    CCCCTGAGCA CTGGCTACAA TACCCGCAGC AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG
    GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC GCTGGCGGCC GCGCCCGAAT GCGCACGGCC
    AGCGAGCTGC TGGTGGACAG GCGCGTGCAC ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC
    GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG CTGGAGGCCC TGTACCAGTG CGTGGCTGCC
    CACTCGGAGC TGCTGTGCTA CTTCGTGATC GTCCTCAACC ACATGGTCAC TGCCTCAGCT
    ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC
    AGCAAGCGCT TCTGGATGAC GGCCATCATC TTCACGGAGG TCACAGTGGT CGCCAAGTAT
    CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGCCACGCTG TGCTGCGGCG CTATGAAAAC
    AAGCCCTACT TCCCGCCGCG CATCCTGGGC CTGGAGAAGA GCGACAGCTA CGTCAAGTAC
    GACCTGCTGC AGCTCATGGC GCTATTCTTC CACCGCGCAC AGCTGCTGTG CTACGGCCTC
    TGGGACCATG AGGACCCCCC ATTGTCCAAG GAGCACGATA GGGGCAGTGC AAAGGAGAAG
    GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG
    CCCCCTGCTG AGGACAATGT CCAGGCGGAA GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC
    AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG AGGAGGAAGG AGAGCACGGA AACCAGAGGA
    CAGGCCATTG AAGGCGAGGA AGAGGAGGAG GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG
    GCAGCGACCT TGCGGAGAGA GAAGAGGCGG AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG
    GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC CTGGCCCGGA GCATGTACTG GCCCGTGCGG
    CGCTTCTTCC AGGACATCCT GCACACGAAG TACCGGGCCG CGACCGACGT CTACGCGCTC
    ATGTTCTTGG CCGACGTCAT CGACTTCATC ATCATCATCT TCGGATTCTG GGCCTTTGGG
    AAGCACTCGG CAGCCACGGA CATCACGTCC TCCTTGTCAG ATGACCAGGT GCCCGAGGCC
    TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC ACCATGGTCA TTGACCGCGC CCTCTACCTG
    CGCAAGACCG TGCTGGGCAA GCTGGCCTTC CAGGTCGTCC TGGTGCTGGC CGTCCACCTC
    TGGATGTTCT TCATCCTGCC CGCTGTCACT GAGCGGATGT TCAGCCAGAA CGCGGTGGCC
    CAGCTGTGGT ACTTTGTGAA ATGCATCTAC TTCGCTCTGT CCGCCTACCA GATCCGCTGT
    GGCTACCCCA CCCGCATCCT TGGCAACTTC CTCACCAAGA AGTACAACCA CCTCAACCTT
    TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA TTCCTGGTGG AGCTGCGGGC AGTGATGGAC
    TGGGTGTGGA CGGACACCAC GCTGTCCCTG TCCAACTGGA TGTGCGTGGA GGACATCTAC
    GCCAACATCT TCATCATCAA GTGCAGCCGA GAGACTGAGA AGAAATACCC GCAGCCCAAG
    GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC GGCATGGGCG GCCTCATCAT CCTGTTCCTC
    GTGGCCATCA TCTGGTTCCC GCTGCTCTTC ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC
    GTCAACCAGC CCATCGACGT CACCGTCACC CTCAAGCTGG GCGGCTATGA GGTGAGCATC
    ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC
    AGGTCACCAT GTCCCCACCA CCAGCCCCTG TTCACCATGA GCGCCCAGCA GCCATCCATC
    GTGCCCTTCA CGCACCACGC CTACGAGGAG CTGTCCAAGC AGTTTGACCC CCACCCACTG
    GCCATGCAGT TCATCAGCCA GTACAGCCCT GAGGATATCG TCACAGTGCA CATCGAGGGC
    AGCTCCGGGG CACTGTGGCG CATCAGCCCG CCAAGCCGGG CACAGATGAA GCGGGAGCTG
    TATAACGGCA CGGCCGACAT CACCCTGCGC TTCACCTGGA ACTTCCAGAG GGACCTGGCC
    AAGGGCGGCA GCGTGGAGTA CACCAACGAG AAGCACACCC TGGACCTGGC CCCTAACAGC
    ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG GAAGGCACCT CGGACCAGTC CGTGGTCATC
    CCCCACCTCT TCCCCAAGTA CATCCGTGCC CCCAATGGGC CTGAAGCCAA CCCCGTGAAG
    CAGCTGCAGC CCAACGAGGA GGCTGACTAT CTCGGCGTGC GTATCCAGCT GCGCAGGGAG
    CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC GAGTGGTGGG TCATTGAGCT GCAGGACTGC
    CAGGCCAACT GCAACCTACT GCCCATGGTC ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC
    CTGGGCTTCC TGGCCGGCTA TGGCATCATG GGGCTGTACG TGTCCATCGT GCTGGTCATC
    GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG ATCTCGCACT CCATCATGTT TGAGGAGCTG
    CCTTGCGTGG ACCGCATCCT CAAGCTATGC CAGGACATCT TCCTGGTGCG GGAGACGCGG
    GAGCTGGAGC TGGAGGAGGA GCTCTACGCC AAGCTCATCT TCCTGTACCG CTCACCCGAG
    ACCATGATCA AGTGGACGCG CGACAAGGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93380
    Clone ID Related Accession (Same CDS sequence) XM_015467309.1 , XM_015475783.1
    Accession Version XM_015475783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGT TATCCACTCT
    GGGGACTATT TCCTGTTCGA GTCGGACAGT GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC
    CCCAGGCCAT CGTCACAGAG CGCCTTTCAG ATGGCCTACC AGGCGTGGGT GACCAACGCG
    CAGACGGTGC TTCGGCAGCA GCGGCAGCAG AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC
    CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC TTGGAGGACG AGGTGACCGG CCGCAGCCAT
    GTGATGCAGA GAGTCCTGAG CGCCGTGCAG TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG
    GACGGGCTGA CGCGCTGGCT GCACGACTTC ACGCGGCACC ACCGCGCCAT AAGCGACGTA
    CTGCGCGCAG AGCGCTACCT GCTCACGCAG GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG
    GACGTGCTGG ACCAGCTGTA CGCCAACGAA GCTGAGGCAG CCCGTGATGC GCTGAGCACA
    GCATCGAGCG GGCTGGGGGT GGAGGAGCCG CTGAGCAGCG TGACCGAGGA CACCAGCAGC
    CCCCTGAGCA CTGGCTACAA TACCCGCAGC AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG
    GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC GCTGGCGGCC GCGCCCGAAT GCGCACGGCC
    AGCGAGCTGC TGGTGGACAG GCGCGTGCAC ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC
    GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG CTGGAGGCCC TGTACCAGTG CGTGGCTGCC
    CACTCGGAGC TGCTGTGCTA CTTCGTGATC GTCCTCAACC ACATGGTCAC TGCCTCAGCT
    ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC
    AGCAAGCGCT TCTGGATGAC GGCCATCATC TTCACGGAGG TCACAGTGGT CGCCAAGTAT
    CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGCCACGCTG TGCTGCGGCG CTATGAAAAC
    AAGCCCTACT TCCCGCCGCG CATCCTGGGC CTGGAGAAGA GCGACAGCTA CGTCAAGTAC
    GACCTGCTGC AGCTCATGGC GCTATTCTTC CACCGCGCAC AGCTGCTGTG CTACGGCCTC
    TGGGACCATG AGGACCCCCC ATTGTCCAAG GAGCACGATA GGGGCAGTGC AAAGGAGAAG
    GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG
    CCCCCTGCTG AGGACAATGT CCAGGCGGAA GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC
    AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG AGGAGGAAGG AGAGCACGGA AACCAGAGGA
    CAGGCCATTG AAGGCGAGGA AGAGGAGGAG GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG
    GCAGCGACCT TGCGGAGAGA GAAGAGGCGG AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG
    GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC CTGGCCCGGA GCATGTACTG GCCCGTGCGG
    CGCTTCTTCC AGGACATCCT GCACACGAAG TACCGGGCCG CGACCGACGT CTACGCGCTC
    ATGTTCTTGG CCGACGTCAT CGACTTCATC ATCATCATCT TCGGATTCTG GGCCTTTGGG
    AAGCACTCGG CAGCCACGGA CATCACGTCC TCCTTGTCAG ATGACCAGGT GCCCGAGGCC
    TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC ACCATGGTCA TTGACCGCGC CCTCTACCTG
    CGCAAGACCG TGCTGGGCAA GCTGGCCTTC CAGGTCGTCC TGGTGCTGGC CGTCCACCTC
    TGGATGTTCT TCATCCTGCC CGCTGTCACT GAGCGGATGT TCAGCCAGAA CGCGGTGGCC
    CAGCTGTGGT ACTTTGTGAA ATGCATCTAC TTCGCTCTGT CCGCCTACCA GATCCGCTGT
    GGCTACCCCA CCCGCATCCT TGGCAACTTC CTCACCAAGA AGTACAACCA CCTCAACCTT
    TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA TTCCTGGTGG AGCTGCGGGC AGTGATGGAC
    TGGGTGTGGA CGGACACCAC GCTGTCCCTG TCCAACTGGA TGTGCGTGGA GGACATCTAC
    GCCAACATCT TCATCATCAA GTGCAGCCGA GAGACTGAGA AGAAATACCC GCAGCCCAAG
    GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC GGCATGGGCG GCCTCATCAT CCTGTTCCTC
    GTGGCCATCA TCTGGTTCCC GCTGCTCTTC ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC
    GTCAACCAGC CCATCGACGT CACCGTCACC CTCAAGCTGG GCGGCTATGA GGTGAGCATC
    ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC
    AGGTCACCAT GTCCCCACCA CCAGCCCCTG TTCACCATGA GCGCCCAGCA GCCATCCATC
    GTGCCCTTCA CGCACCACGC CTACGAGGAG CTGTCCAAGC AGTTTGACCC CCACCCACTG
    GCCATGCAGT TCATCAGCCA GTACAGCCCT GAGGATATCG TCACAGTGCA CATCGAGGGC
    AGCTCCGGGG CACTGTGGCG CATCAGCCCG CCAAGCCGGG CACAGATGAA GCGGGAGCTG
    TATAACGGCA CGGCCGACAT CACCCTGCGC TTCACCTGGA ACTTCCAGAG GGACCTGGCC
    AAGGGCGGCA GCGTGGAGTA CACCAACGAG AAGCACACCC TGGACCTGGC CCCTAACAGC
    ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG GAAGGCACCT CGGACCAGTC CGTGGTCATC
    CCCCACCTCT TCCCCAAGTA CATCCGTGCC CCCAATGGGC CTGAAGCCAA CCCCGTGAAG
    CAGCTGCAGC CCAACGAGGA GGCTGACTAT CTCGGCGTGC GTATCCAGCT GCGCAGGGAG
    CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC GAGTGGTGGG TCATTGAGCT GCAGGACTGC
    CAGGCCAACT GCAACCTACT GCCCATGGTC ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC
    CTGGGCTTCC TGGCCGGCTA TGGCATCATG GGGCTGTACG TGTCCATCGT GCTGGTCATC
    GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG ATCTCGCACT CCATCATGTT TGAGGAGCTG
    CCTTGCGTGG ACCGCATCCT CAAGCTATGC CAGGACATCT TCCTGGTGCG GGAGACGCGG
    GAGCTGGAGC TGGAGGAGGA GCTCTACGCC AAGCTCATCT TCCTGTACCG CTCACCCGAG
    ACCATGATCA AGTGGACGCG CGACAAGGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331269.1
    CDS167..7099
    Translation

    Target ORF information:

    RefSeq Version XM_015475783.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGT TATCCACTCT
    GGGGACTATT TCCTGTTCGA GTCGGACAGT GAGGAGGAGG AGGAAGCCCA GCCCGAGGAC
    CCCAGGCCAT CGTCACAGAG CGCCTTTCAG ATGGCCTACC AGGCGTGGGT GACCAACGCG
    CAGACGGTGC TTCGGCAGCA GCGGCAGCAG AGGGCCGAGC AGCTGCGCAC AGGAGGCGAC
    CCAAGCCAGG AAGCAGGGCC GGCAGAGGGC TTGGAGGACG AGGTGACCGG CCGCAGCCAT
    GTGATGCAGA GAGTCCTGAG CGCCGTGCAG TTCCTGTGGG TGCTGGGCCA GGCGCTGGTG
    GACGGGCTGA CGCGCTGGCT GCACGACTTC ACGCGGCACC ACCGCGCCAT AAGCGACGTA
    CTGCGCGCAG AGCGCTACCT GCTCACGCAG GAGCTGCTCC GGGGCGGAGA GGTGCGCCGG
    GACGTGCTGG ACCAGCTGTA CGCCAACGAA GCTGAGGCAG CCCGTGATGC GCTGAGCACA
    GCATCGAGCG GGCTGGGGGT GGAGGAGCCG CTGAGCAGCG TGACCGAGGA CACCAGCAGC
    CCCCTGAGCA CTGGCTACAA TACCCGCAGC AGCAGCGAAG AGGTGGTCAC CGAGCCTGGG
    GCTTCTCTGC GCAGCTCTGG GGAGCTTCCC GCTGGCGGCC GCGCCCGAAT GCGCACGGCC
    AGCGAGCTGC TGGTGGACAG GCGCGTGCAC ATTCCGGAGC TGGAGGAGGC GGAGCAGTTC
    GCGGCGGGGC GGGGCCGGGC GCTTCGGCTG CTGGAGGCCC TGTACCAGTG CGTGGCTGCC
    CACTCGGAGC TGCTGTGCTA CTTCGTGATC GTCCTCAACC ACATGGTCAC TGCCTCAGCT
    ACCTCCCTTG TGCTGCCCGT GCTGGTCTTC CTGTGGGCCA TGCTCTCCAT CCCGCGGCCC
    AGCAAGCGCT TCTGGATGAC GGCCATCATC TTCACGGAGG TCACAGTGGT CGCCAAGTAT
    CTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGCCACGCTG TGCTGCGGCG CTATGAAAAC
    AAGCCCTACT TCCCGCCGCG CATCCTGGGC CTGGAGAAGA GCGACAGCTA CGTCAAGTAC
    GACCTGCTGC AGCTCATGGC GCTATTCTTC CACCGCGCAC AGCTGCTGTG CTACGGCCTC
    TGGGACCATG AGGACCCCCC ATTGTCCAAG GAGCACGATA GGGGCAGTGC AAAGGAGAAG
    GGGGCTGAGG AGGAGCCAGC CCTGCCTGCG CCCCAGGAGG AGCCAGGAGG GGCGGCCAGG
    CCCCCTGCTG AGGACAATGT CCAGGCGGAA GCGAAGGGTG GGCCCCTAGA GCCCGGCGGC
    AAGAGGCGCA TCAGCCTCCG TTTCAGGAGG AGGAGGAAGG AGAGCACGGA AACCAGAGGA
    CAGGCCATTG AAGGCGAGGA AGAGGAGGAG GAGGCAGCAG TGGCGGTGGT GGCGGCGGGG
    GCAGCGACCT TGCGGAGAGA GAAGAGGCGG AGTCGCCCCC AGGAAAGAGT GAGGGTGCTG
    GGGGTCCGGC TGCAGAGCTT CTGCCTGTCC CTGGCCCGGA GCATGTACTG GCCCGTGCGG
    CGCTTCTTCC AGGACATCCT GCACACGAAG TACCGGGCCG CGACCGACGT CTACGCGCTC
    ATGTTCTTGG CCGACGTCAT CGACTTCATC ATCATCATCT TCGGATTCTG GGCCTTTGGG
    AAGCACTCGG CAGCCACGGA CATCACGTCC TCCTTGTCAG ATGACCAGGT GCCCGAGGCC
    TTCCTGGTCA TGCTGCTGAT CCAGTTCAGC ACCATGGTCA TTGACCGCGC CCTCTACCTG
    CGCAAGACCG TGCTGGGCAA GCTGGCCTTC CAGGTCGTCC TGGTGCTGGC CGTCCACCTC
    TGGATGTTCT TCATCCTGCC CGCTGTCACT GAGCGGATGT TCAGCCAGAA CGCGGTGGCC
    CAGCTGTGGT ACTTTGTGAA ATGCATCTAC TTCGCTCTGT CCGCCTACCA GATCCGCTGT
    GGCTACCCCA CCCGCATCCT TGGCAACTTC CTCACCAAGA AGTACAACCA CCTCAACCTT
    TTCCTCTTCC AGGGGTTCCG GCTGGTGCCA TTCCTGGTGG AGCTGCGGGC AGTGATGGAC
    TGGGTGTGGA CGGACACCAC GCTGTCCCTG TCCAACTGGA TGTGCGTGGA GGACATCTAC
    GCCAACATCT TCATCATCAA GTGCAGCCGA GAGACTGAGA AGAAATACCC GCAGCCCAAG
    GGGCAGAAGA AGAAAAAGAT TGTCAAGTAC GGCATGGGCG GCCTCATCAT CCTGTTCCTC
    GTGGCCATCA TCTGGTTCCC GCTGCTCTTC ATGTCCCTGG TGCGCTCTGT GGTCGGCGTC
    GTCAACCAGC CCATCGACGT CACCGTCACC CTCAAGCTGG GCGGCTATGA GGTGAGCATC
    ATAAGGGGGT CTGGCTGCAC GTTGCTGCTG TGGCGGGGCT GGGGGGCCTC TCCGGGCGCC
    AGGTCACCAT GTCCCCACCA CCAGCCCCTG TTCACCATGA GCGCCCAGCA GCCATCCATC
    GTGCCCTTCA CGCACCACGC CTACGAGGAG CTGTCCAAGC AGTTTGACCC CCACCCACTG
    GCCATGCAGT TCATCAGCCA GTACAGCCCT GAGGATATCG TCACAGTGCA CATCGAGGGC
    AGCTCCGGGG CACTGTGGCG CATCAGCCCG CCAAGCCGGG CACAGATGAA GCGGGAGCTG
    TATAACGGCA CGGCCGACAT CACCCTGCGC TTCACCTGGA ACTTCCAGAG GGACCTGGCC
    AAGGGCGGCA GCGTGGAGTA CACCAACGAG AAGCACACCC TGGACCTGGC CCCTAACAGC
    ACTGCCCGGC GGCAGCTGGC CAGCCTGCTG GAAGGCACCT CGGACCAGTC CGTGGTCATC
    CCCCACCTCT TCCCCAAGTA CATCCGTGCC CCCAATGGGC CTGAAGCCAA CCCCGTGAAG
    CAGCTGCAGC CCAACGAGGA GGCTGACTAT CTCGGCGTGC GTATCCAGCT GCGCAGGGAG
    CGTGTGGGCA CAGGGGCAGC TGGCTTCCTC GAGTGGTGGG TCATTGAGCT GCAGGACTGC
    CAGGCCAACT GCAACCTACT GCCCATGGTC ATCTTCAGTG ACAAGGTCAG CCCGCCCAGC
    CTGGGCTTCC TGGCCGGCTA TGGCATCATG GGGCTGTACG TGTCCATCGT GCTGGTCATC
    GGCAAGTTCG TGCGCGGCTT CTTCAGCGAG ATCTCGCACT CCATCATGTT TGAGGAGCTG
    CCTTGCGTGG ACCGCATCCT CAAGCTATGC CAGGACATCT TCCTGGTGCG GGAGACGCGG
    GAGCTGGAGC TGGAGGAGGA GCTCTACGCC AAGCTCATCT TCCTGTACCG CTCACCCGAG
    ACCATGATCA AGTGGACGCG CGACAAGGAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93381
    Clone ID Related Accession (Same CDS sequence) XM_015475784.1 , XM_015467310.1
    Accession Version XM_015475784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAATGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331270.1
    CDS166..7077
    Translation

    Target ORF information:

    RefSeq Version XM_015475784.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAATGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93381
    Clone ID Related Accession (Same CDS sequence) XM_015475784.1 , XM_015467310.1
    Accession Version XM_015467310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAATGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322796.1
    CDS172..7083
    Translation

    Target ORF information:

    RefSeq Version XM_015467310.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGCCCCTGT TCACCATGAG CGCCCAGCAG CCATCCATCG TGCCCTTCAC GCACCACGCC
    TACGAGGAGC TGTCCAAGCA GTTTGACCCC CACCCACTGG CCATGCAGTT CATCAGCCAG
    TACAGCCCTG AGGATATCGT CACAGTGCAC ATCGAGGGCA GCTCCGGGGC ACTGTGGCGC
    ATCAGCCCGC CAAGCCGGGC ACAGATGAAG CGGGAGCTGT ATAACGGCAC GGCCGACATC
    ACCCTGCGCT TCACCTGGAA CTTCCAGAGG GACCTGGCCA AGGGCGGCAG CGTGGAGTAC
    ACCAACGAGA AGCACACCCT GGACCTGGCC CCTAACAGCA CTGCCCGGCG GCAGCTGGCC
    AGCCTGCTGG AAGGCACCTC GGACCAGTCC GTGGTCATCC CCCACCTCTT CCCCAAGTAC
    ATCCGTGCCC CCAATGGGCC TGAAGCCAAC CCCGTGAAGC AGCTGCAGCC CAACGAGGAG
    GCTGACTATC TCGGCGTGCG TATCCAGCTG CGCAGGGAGC GTGTGGGCAC AGGGGCAGCT
    GGCTTCCTCG AGTGGTGGGT CATTGAGCTG CAGGACTGCC AGGCCAACTG CAACCTACTG
    CCCATGGTCA TCTTCAGTGA CAAGGTCAGC CCGCCCAGCC TGGGCTTCCT GGCCGGCTAT
    GGCATCATGG GGCTGTACGT GTCCATCGTG CTGGTCATCG GCAAGTTCGT GCGCGGCTTC
    TTCAGCGAGA TCTCGCACTC CATCATGTTT GAGGAGCTGC CTTGCGTGGA CCGCATCCTC
    AAGCTATGCC AGGACATCTT CCTGGTGCGG GAGACGCGGG AGCTGGAGCT GGAGGAGGAG
    CTCTACGCCA AGCTCATCTT CCTGTACCGC TCACCCGAGA CCATGATCAA GTGGACGCGC
    GACAAGGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93382
    Clone ID Related Accession (Same CDS sequence) XM_015467311.1 , XM_015475785.1
    Accession Version XM_015467311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGCAT
    CATGGGGCTG TACGTGTCCA TCGTGCTGGT CATCGGCAAG TTCGTGCGCG GCTTCTTCAG
    CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322797.1
    CDS175..6981
    Translation

    Target ORF information:

    RefSeq Version XM_015467311.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGCAT
    CATGGGGCTG TACGTGTCCA TCGTGCTGGT CATCGGCAAG TTCGTGCGCG GCTTCTTCAG
    CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93382
    Clone ID Related Accession (Same CDS sequence) XM_015467311.1 , XM_015475785.1
    Accession Version XM_015475785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product piezo-type mechanosensitive ion channel component 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGCAT
    CATGGGGCTG TACGTGTCCA TCGTGCTGGT CATCGGCAAG TTCGTGCGCG GCTTCTTCAG
    CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331271.1
    CDS168..6974
    Translation

    Target ORF information:

    RefSeq Version XM_015475785.1
    Organism Bos taurus(cattle)
    Definition Bos taurus piezo-type mechanosensitive ion channel component 1 (PIEZO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGCTGCTCG CACTGGCAGG CATTGCCCAC CCCTCAGCCT TCTCCAGTGT CTACTTCCTG 
    CTCTTCCTGG CCATTGGCAC TTGGTGGGCC TGTCACTTCC CCATCAGCCT GTTGGGCTTC
    AACACACTCT GCGTCATGGT GAGCTGCATT GGCACCGGCC ATCTCATCTG CCTCTATTGC
    TACCAGACAC CTCTCGCCCA GGCCACTCTC CCACCTGCTG GCATCTGGGC CAGGGTGTTT
    GGTCTCAAGG ACTTTTTGGC CCCCACCAAC TGCTCCAGCC CCAACGTGCT GGTCATCAAT
    GCTAACCACG ACTGGCCCGT GTACGTGAGC CCCGGCATCC TGCTGCTGCT CTGCTACACG
    GTGACCTCGC TCTTGAAGCT CCATGCCCAC CAGCCTGTGG ACCAGAGGAA GGAGGCGGCC
    GGGGACGATG ACACACGGGA GGTGGAGTTG ACAGAGGTGG ACCAGTGGCC CCAGGGTCAG
    GCCAGAGTTG TGGCCATCAA GGAGGAAGGG GCTGCCCAGC ACACAATGCC TACTCCCACA
    ACACCAGACC CCGAGGACAA CTGCATCATA CACGACCTCA CTGGCCACAG CGCCGTCCAG
    CAGCGAACCT CATGCCCCCG GCTGGCTGAG CCAAAGGAGA CGTCTCCGCT GCACGGCCTG
    GGCCACCTCA TCATGGACCA GAGCTACGTG TGTGCCCTCA TCGCCATGAT GGTGTGGAGC
    ATCACCTACC ACAGCTGGCT GACCTTCGTG CTGCTGCTCT GGGCCTGCCT CATCTGGACG
    GTACGCAGCC GCCACCAGCT GGCCATGCTC TGCTCGCCCT TCATCCTGCT CTATGGGCTG
    GCACTCTGCT GCCTGCGTTA CGTGTGGGCC ATGGACCTGC GGCCCGAGCT GCCCACCGCC
    CTGGGCCCCG TCAGCCTGCG CCAGCTGGGG CTGGAGCACA CCCGCTACCC CTGCCTGGAC
    CTGGGTGCCA TGCTGCTGTG CACCCTGACC TTCTGGCTCC TGCTGCGCCA GTTTGTGAAG
    GAGAAGCTGC TGAGGAGGGC ACGGGCCCCT GTGGCGCTGA CGGAGGTCAC CGTGGCCGCC
    ACGGAGCCCA CGCGGACGCA GATGCTGCTG CGGAGCCTGG GGGAGCTGGT CAGGGGCATC
    TACGCCAAGT ACTGGATCTA CGTGTGCGCA GGCATGTTCA TCGTGGTCAG CTTTGCTGGC
    CGCCTGGTGG TCTACAAGAT CGTCTACATG TTGCTCTTCC TGCTCTGTCT CATCCTCTTC
    CAGGTCTACT ACAGCCTGTG GCGGAAGCTG CTCAAGGCAT TCTGGTGGCT GGTGGTGGCC
    TACACCATGC TGGTGTTGGT GGCCGTCTAC ACCTTCCAGT TCCAGGACTT CCCGGCCTAC
    TGGCGCAACC TGACGGGCCT CACCGATGAG CAGCTGGGGG ACTTGGGCCT GGAGCAGTTC
    AGCGTGTCCG AGCTCTTCTC CAGCATCCTG GTCCCCGGCT TCTTCCTGCT TGCCTGCATT
    CTGCAGCTGC ACTACTTCCA CCGGCCCTTC ATGCAGCTCA CCGACCCCGA GCACCGGCCC
    CTACCTGGTG CCTGCACCCC ACGCTGGGTT CCCGGGCGGG ACGCGGTGAG CCGGACCCCC
    CTGCTGCAGC AGGAGGAAGA GGAGGAGGTA CCCAGGGACG AGGGGCTGGG CACGGCCAGC
    CCCCACCAGG TCACACAGGT CCCAGAGGCC AGCAAGTGGG GCCTGGTGGC TGAGCGGCTG
    CTGGACCTGG CCACCGGCTT CTCGGACGTC ATCACCCGCG TGCAGGTGCT GGTGCGGCGC
    CTGCTGGAGC TGCACGTCTT CAAGCTGGTG GCCCTGTACA CCGTGTGGGT GGCCCTGAAG
    GAGGTGTCGG TGCTGAACTT CCTGCTGGTC GTGCTCTGGG CCTTCGCCCT GCCCTACCCA
    CGCTTCCGGC CCATGGCCTC ATGCCTGGCC ACTGTGTGGA CCTGCATCAT CATCGTGTGC
    AAGATGCTGT ACCAGCTCAA GGTGGTCAAC CCCCACGAGT ACGCCAGCAA CTGCACGGAG
    CCCTTCCCCA ATAGTACGAA CCTGCAGAAG ACGGAGATCC GCCAGTCCTT GCTGTATCGC
    GGGCCTGTTG ACCCTGCCAA CTGGTTTGGG GTGCGGAAGG GCTTCCCCAA CCTGGGCTAC
    ATCCAGAACC ACCTGCAGAT CCTGCTGCTG CTGGTGTTTG AGGCCATGGT GTACCGGAGC
    CAGGACTACC ACCACCGCCG GCACCAGCTG GCCCCGCTGC CTGCCCAGGC TGTCTGCGCC
    GAGGGCACCC GCCAGCGGCT GGACCGCGAC CTGCCCAGCT GCCTCAAGTA CTACGTGAAC
    TTCTTCTTCT ACAAATTTGG GCTGGAGGTC TGCTTCCTGG CAGCCGTGAA CGTGATCGGG
    CAGCGCATGA ACTTCATGGT CATCCTGCAC GGCTGCTGGC TGGTGGCCAT CCTTACCCGC
    CGACGCCGGG AGGCCATCGC CCGCCTCTGG CCCAATTACT GCCTGTTCCT GGCGCTCTTC
    CTGCTGTACC AGTACCTGCT GTGCCTGGGT GTTCCCCCCG CCCTGTGCAT TGACTACCCA
    TGGCGCTGGA GCCGGGCCGT CCCCATGAAC TCCGCGCTCA TCAAGTGGCT ATACCTGCCT
    GATTTCTTCA GCACCCCCAA TGCCACCAAC CTCATCAGTG ACTTCCTGCT GCTGCTCTGC
    GCCTCCCAGC AGTGGCAGGT GTTCTCGGCC GAGCGCATGG AGGAGTGGCG GCACATGGCT
    GGCGTCAATA CTGACCGCCT GGAGCCTCTG CGGGGGGAGC CCAACCCCGT GCCCAACTTC
    ATCCACTGCA GGTCCTACCT CGACATGCTG AAAGTGGCTG TCTTCCGCTA CTTCTTCTGG
    CTGGTGCTGG TGGTGGTGTT CATCACGGGG GCCACGCGCA TCAGCGTCTT TGGGCTGGGC
    TACCTACTGG CCTGCTTCTA CTTGCTGCTC TTCGGCACCA GCCTGCAGCA GAAGGACACG
    CGTGCCCGCC TCGTGCTGTG GGACTGCCTC ATCCTTTACA ATGTCACTGT CATCGTCTCC
    AAGAACATGC TCTCTCTCCT GTCCTGCGTC TTCGTGGAGC AGATGCAGAG CAGCTTCTGC
    TGGGTCATCC AGCTCTTCAG CCTCGTATGC ACCGTCAAGG GCTACTACGA CCCCAAGGAG
    ATGCTGGGCC GCGACCAGGA CTGCCTGCTG CCCGTGGAGG AGGCGGGCGT CCTGTGGGAC
    AGCGTCTGCT TCCTGTTTCT GCTGCTGCAG CGCAGGGTGT TCCTCAGCTA CTACTTCCTG
    CACGTTCAGG CCGAGCTCCG CGCCACCGCC CTGCAGGCCT CCAGGGGCTT TGCCCTCTAC
    AATGCTGCCA ATCTCAAGAC CATCGAGCTC CACCGCAGAG CGGAGGAGAA GTCACTGGCC
    CAGCTGAAAA GACAGATGGA GCGGATCCGG GCCAAGCAAG AGAAGCACAG GCAGGGCCGG
    GCCAACCGAG GCTGCCTTCA GGGCTCCCCG GATCCCGGCC AAGAGCCAGG GCCCAGCAGC
    CCAGGGGGCT CCTCCCCGCC ACAACAACAG TGGTGGCGGC CCTGGCTGGA CCACGCCACA
    GTTATCCACT CTGGGGACTA TTTCCTGTTC GAGTCGGACA GTGAGGAGGA GGAGGAAGCC
    CAGCCCGAGG ACCCCAGGCC ATCGTCACAG AGCGCCTTTC AGATGGCCTA CCAGGCGTGG
    GTGACCAACG CGCAGACGGT GCTTCGGCAG CAGCGGCAGC AGAGGGCCGA GCAGCTGCGC
    ACAGGAGGCG ACCCAAGCCA GGAAGCAGGG CCGGCAGAGG GCTTGGAGGA CGAGGTGACC
    GGCCGCAGCC ATGTGATGCA GAGAGTCCTG AGCGCCGTGC AGTTCCTGTG GGTGCTGGGC
    CAGGCGCTGG TGGACGGGCT GACGCGCTGG CTGCACGACT TCACGCGGCA CCACCGCGCC
    ATAAGCGACG TACTGCGCGC AGAGCGCTAC CTGCTCACGC AGGAGCTGCT CCGGGGCGGA
    GAGGTGCGCC GGGACGTGCT GGACCAGCTG TACGCCAACG AAGCTGAGGC AGCCCGTGAT
    GCGCTGAGCA CAGCATCGAG CGGGCTGGGG GTGGAGGAGC CGCTGAGCAG CGTGACCGAG
    GACACCAGCA GCCCCCTGAG CACTGGCTAC AATACCCGCA GCAGCAGCGA AGAGGTGGTC
    ACCGAGCCTG GGGCTTCTCT GCGCAGCTCT GGGGAGCTTC CCGCTGGCGG CCGCGCCCGA
    ATGCGCACGG CCAGCGAGCT GCTGGTGGAC AGGCGCGTGC ACATTCCGGA GCTGGAGGAG
    GCGGAGCAGT TCGCGGCGGG GCGGGGCCGG GCGCTTCGGC TGCTGGAGGC CCTGTACCAG
    TGCGTGGCTG CCCACTCGGA GCTGCTGTGC TACTTCGTGA TCGTCCTCAA CCACATGGTC
    ACTGCCTCAG CTACCTCCCT TGTGCTGCCC GTGCTGGTCT TCCTGTGGGC CATGCTCTCC
    ATCCCGCGGC CCAGCAAGCG CTTCTGGATG ACGGCCATCA TCTTCACGGA GGTCACAGTG
    GTCGCCAAGT ATCTGTTCCA GTTTGGCTTC TTCCCCTGGA ACAGCCACGC TGTGCTGCGG
    CGCTATGAAA ACAAGCCCTA CTTCCCGCCG CGCATCCTGG GCCTGGAGAA GAGCGACAGC
    TACGTCAAGT ACGACCTGCT GCAGCTCATG GCGCTATTCT TCCACCGCGC ACAGCTGCTG
    TGCTACGGCC TCTGGGACCA TGAGGACCCC CCATTGTCCA AGGAGCACGA TAGGGGCAGT
    GCAAAGGAGA AGGGGGCTGA GGAGGAGCCA GCCCTGCCTG CGCCCCAGGA GGAGCCAGGA
    GGGGCGGCCA GGCCCCCTGC TGAGGACAAT GTCCAGGCGG AAGCGAAGGG TGGGCCCCTA
    GAGCCCGGCG GCAAGAGGCG CATCAGCCTC CGTTTCAGGA GGAGGAGGAA GGAGAGCACG
    GAAACCAGAG GACAGGCCAT TGAAGGCGAG GAAGAGGAGG AGGAGGCAGC AGTGGCGGTG
    GTGGCGGCGG GGGCAGCGAC CTTGCGGAGA GAGAAGAGGC GGAGTCGCCC CCAGGAAAGA
    GTGAGGGTGC TGGGGGTCCG GCTGCAGAGC TTCTGCCTGT CCCTGGCCCG GAGCATGTAC
    TGGCCCGTGC GGCGCTTCTT CCAGGACATC CTGCACACGA AGTACCGGGC CGCGACCGAC
    GTCTACGCGC TCATGTTCTT GGCCGACGTC ATCGACTTCA TCATCATCAT CTTCGGATTC
    TGGGCCTTTG GGAAGCACTC GGCAGCCACG GACATCACGT CCTCCTTGTC AGATGACCAG
    GTGCCCGAGG CCTTCCTGGT CATGCTGCTG ATCCAGTTCA GCACCATGGT CATTGACCGC
    GCCCTCTACC TGCGCAAGAC CGTGCTGGGC AAGCTGGCCT TCCAGGTCGT CCTGGTGCTG
    GCCGTCCACC TCTGGATGTT CTTCATCCTG CCCGCTGTCA CTGAGCGGAT GTTCAGCCAG
    AACGCGGTGG CCCAGCTGTG GTACTTTGTG AAATGCATCT ACTTCGCTCT GTCCGCCTAC
    CAGATCCGCT GTGGCTACCC CACCCGCATC CTTGGCAACT TCCTCACCAA GAAGTACAAC
    CACCTCAACC TTTTCCTCTT CCAGGGGTTC CGGCTGGTGC CATTCCTGGT GGAGCTGCGG
    GCAGTGATGG ACTGGGTGTG GACGGACACC ACGCTGTCCC TGTCCAACTG GATGTGCGTG
    GAGGACATCT ACGCCAACAT CTTCATCATC AAGTGCAGCC GAGAGACTGA GAAGAAATAC
    CCGCAGCCCA AGGGGCAGAA GAAGAAAAAG ATTGTCAAGT ACGGCATGGG CGGCCTCATC
    ATCCTGTTCC TCGTGGCCAT CATCTGGTTC CCGCTGCTCT TCATGTCCCT GGTGCGCTCT
    GTGGTCGGCG TCGTCAACCA GCCCATCGAC GTCACCGTCA CCCTCAAGCT GGGCGGCTAT
    GAGGTGAGCA TCATAAGGGG GTCTGGCTGC ACGTTGCTGC TGTGGCGGGG CTGGGGGGCC
    TCTCCGGGCG CCAGGTCACC ATGTCCCCAC CACCAGCCCC TGTTCACCAT GAGCGCCCAG
    CAGCCATCCA TCGTGCCCTT CACGCACCAC GCCTACGAGG AGCTGTCCAA GCAGTTTGAC
    CCCCACCCAC TGGCCATGCA GTTCATCAGC CAGTACAGCC CTGAGGATAT CGTCACAGTG
    CACATCGAGG GCAGCTCCGG GGCACTGTGG CGCATCAGCC CGCCAAGCCG GGCACAGATG
    AAGCGGGAGC TGTATAACGG CACGGCCGAC ATCACCCTGC GCTTCACCTG GAACTTCCAG
    AGGGACCTGG CCAAGGGCGG CAGCGTGGAG TACACCAACG AGAAGCACAC CCTGGACCTG
    GCCCCTAACA GCACTGCCCG GCGGCAGCTG GCCAGCCTGC TGGAAGGCAC CTCGGACCAG
    TCCGTGGTCA TCCCCCACCT CTTCCCCAAG TACATCCGTG CCCCCAATGG GCCTGAAGCC
    AACCCCGTGA AGCAGCTGCA GCCCAACGAG GAGGCTGACT ATCTCGGCGT GCGTATCCAG
    CTGCGCAGGG AGCGTGTGGG CACAGGGGCA GCTGGCTTCC TCGAGTGGTG GGTCATTGAG
    CTGCAGGACT GCCAGGCCAA CTGCAACCTA CTGCCCATGG TCATCTTCAG TGACAAGCAT
    CATGGGGCTG TACGTGTCCA TCGTGCTGGT CATCGGCAAG TTCGTGCGCG GCTTCTTCAG
    CGAGATCTCG CACTCCATCA TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.