HCN4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCN4
Entrez Gene ID	785689
Full Name	hyperpolarization activated cyclic nucleotide gated potassium channel 4
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium channel 4 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002700513.4	XP_002700559.2	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
XM_002690493.4	XP_002690539.1	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
XM_024997938.1	XP_024853706.1	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 isoform X1
XM_002690493.5	XP_002690539.2	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 isoform X2

Pathways from BioSystems

Homologies

	HCN4	XP_002804905.1
Mus musculus (house mouse)	Hcn4	NP_001074661.1
Danio rerio (zebrafish)	hcn4	XP_686078.4
Xenopus tropicalis (tropical clawed frog)	hcn4	XP_004916024.1
Pan troglodytes (chimpanzee)	HCN4	XP_003952750.1
Bos taurus (cattle)	HCN4	XP_002690539.1
Gallus gallus (chicken)	HCN4	XP_425050.3
Homo sapiens (human)	HCN4	NP_005468.1
Danio rerio (zebrafish)	hcn4l	XP_684730.5
Rattus norvegicus (Norway rat)	Hcn4	NP_067690.1

Gene Ontology

The following HCN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219813	XM_024997938.1 Latest version!	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa219814	XM_002690493.5 Latest version!	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa23035	XM_002690493.4 Latest version!	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa23035	XM_002700513.4 Latest version!	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCN4 ( XM_024997938.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853706.1 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219813
Clone ID Related Accession (Same CDS sequence)	XM_024997938.1
Accession Version	XM_024997938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG TGAGCTGCCT 
GCCGGGAGGG AGGTGGCGGC GGGGCGGGGG CGGGGGCGGG GTACAGGCTT CTGTCTGCTC 
CCACCCCGGC CTAAGTGCCT GATCTTGTTC GGTGGTCCAG GCAAGAAAAA CTCCATCCTC 
CTCCACAAAG TCCAGCACGA CCTCAACTCA GGCGTCTTCA ACTACCAGGA AAACGAGATC 
ATCCAGCAGA TCGTGCAGCA CGACAGGGAG ATGGCGCACT GCGCGCATCG CGTCCAGGCC 
GCCGCCTCCG CCACGCCGAC CCCCACGCCG GTCATCTGGA CCCCGCTGAT CCAGGCGCCG 
CTGCAGGCCG CCGCCGCCAC AACCTCCGTG GCCATCGCCC TCACTCACCA CCCGCGCCTG 
CCGGCTGCCA TCTTCCGGCC TCCCCCGGGG CCCGGGCTCG GCGGCCTCGG GGCCGGGCAG 
ACGCCCCGGC ACCTGAAGCG GCTGCAGTCT CTGATCCCAT CGGCGCTGGG CTCCGCCTCG 
CCCGCCAGCA GCCCGTCCCA GGTGGACACG CCGTCTTCCT CCTCCTTTCA CATCCAACAA 
CTGGCTGGAT TCTCCGCGCC GGCCGGTCTG AGCCCGCTCC TCCCCTCGTC CAGCTCCTCC 
CCGCCGCCAG GGGTCGGCGG CTCCTCCCCA GCGCCCACGC CTTCCACCGC CGCCGCCGCC 
GCCACCGCCG GGTTCGGCCA CTTCCACAAG GCGCTGGGGG GCTCCCTGTC CTCGTCCGAC 
TCGCCCCTGC TCACCCCGCT GCAGCCGGGC GCCCGCTCCC CGCAGGCCAG CCAGCCGCCG 
CCCCCGCTGC CGGGGGCCCG GGGAGGCCTG GGACTCGCGG AGCCCTTCCT GCCGCCCCCG 
CCGTCGGCCA GGTCCCCGTC GTCCAGCCCC GGGCAGCTGG GCCAGCCTCC CGGGGAGCTG 
TCCCCCGGGC TGGCCGCCGG CCCGCCGAGT ACGCCAGAGA CACCCCCGCG GCAGCCTGAG 
CGGCTGTCCT TGGTGGCAGG GGCCTCAGGG GGGGCTTCTC CTGTCGCCTT CACCCCCCGA 
GGAGGGCCCA GCCCTCCTGG CCACAGCCCG GGCCCTCCAA GAACTTTCCC AAGTGCCCCG 
CCCCGGGCCT CTGGCTCCCA CGGATCCCTG CTCCTGCCCC CTGCATCCAG CCCCCCGCCG 
CCCCAGGTCC CCCAGCGCCG GGGCACGCCG CCCCTCACCC CTGGTCGCCT GACCCAGGAC 
CTCAAGCTCA TCTCAGCCTC TCAGCCGGCC CTCCCCCAGG ACGGGGCGCA GACTCTCCGC 
AGAGCCTCCC CGCACTCCTC TGGGGAGTCG GTGGCAGCCT TCCCGCTCTT TCCCAGAGGC 
GGGGGCGGCA GCGGGGGCAG CGGGGGCCTC GGTCTCCCCG GGAGGCCCTA TGGTGCCGTC 
CCTGGCCAGC ACGTCACTCT GCCTCGGAAG ACATCCTCAG GTTCTTTGCC ACCCCCTCTT 
TCTTTATTTG GGGCAAGAGC CACTTCTTCT GGGGGGCCCC CTCTGACTGC TGCTCCCCAG 
AGGGAACCTG GGGCCAGGTC TGAGCCGGTG CGCTCCAAAC TGCCGTCCAA CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853706.1
CDS	199..3915
Translation	MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTEPRGAAHGAADGEGSARGAGKSSTNGDCRRFRGSLASLGSRGGGGAGAGGGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERSGASAQPAASPPPPPPQQPPQPVPASCEQTSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGELPAGREVAAGRGRGRGTGFCLLPPRPKCLILFGGPGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGGLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGVGGSSPAPTPSTAAAAATAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQASQPPPPLPGARGGLGLAEPFLPPPPSARSPSSSPGQLGQPPGELSPGLAAGPPSTPETPPRQPERLSLVAGASGGASPVAFTPRGGPSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFPLFPRGGGGSGGSGGLGLPGRPYGAVPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAAPQREPGARSEPVRSKLPSNL

Target ORF information:

RefSeq Version	XM_024997938.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997938.1

ORF Insert Sequence:

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG TGAGCTGCCT 
GCCGGGAGGG AGGTGGCGGC GGGGCGGGGG CGGGGGCGGG GTACAGGCTT CTGTCTGCTC 
CCACCCCGGC CTAAGTGCCT GATCTTGTTC GGTGGTCCAG GCAAGAAAAA CTCCATCCTC 
CTCCACAAAG TCCAGCACGA CCTCAACTCA GGCGTCTTCA ACTACCAGGA AAACGAGATC 
ATCCAGCAGA TCGTGCAGCA CGACAGGGAG ATGGCGCACT GCGCGCATCG CGTCCAGGCC 
GCCGCCTCCG CCACGCCGAC CCCCACGCCG GTCATCTGGA CCCCGCTGAT CCAGGCGCCG 
CTGCAGGCCG CCGCCGCCAC AACCTCCGTG GCCATCGCCC TCACTCACCA CCCGCGCCTG 
CCGGCTGCCA TCTTCCGGCC TCCCCCGGGG CCCGGGCTCG GCGGCCTCGG GGCCGGGCAG 
ACGCCCCGGC ACCTGAAGCG GCTGCAGTCT CTGATCCCAT CGGCGCTGGG CTCCGCCTCG 
CCCGCCAGCA GCCCGTCCCA GGTGGACACG CCGTCTTCCT CCTCCTTTCA CATCCAACAA 
CTGGCTGGAT TCTCCGCGCC GGCCGGTCTG AGCCCGCTCC TCCCCTCGTC CAGCTCCTCC 
CCGCCGCCAG GGGTCGGCGG CTCCTCCCCA GCGCCCACGC CTTCCACCGC CGCCGCCGCC 
GCCACCGCCG GGTTCGGCCA CTTCCACAAG GCGCTGGGGG GCTCCCTGTC CTCGTCCGAC 
TCGCCCCTGC TCACCCCGCT GCAGCCGGGC GCCCGCTCCC CGCAGGCCAG CCAGCCGCCG 
CCCCCGCTGC CGGGGGCCCG GGGAGGCCTG GGACTCGCGG AGCCCTTCCT GCCGCCCCCG 
CCGTCGGCCA GGTCCCCGTC GTCCAGCCCC GGGCAGCTGG GCCAGCCTCC CGGGGAGCTG 
TCCCCCGGGC TGGCCGCCGG CCCGCCGAGT ACGCCAGAGA CACCCCCGCG GCAGCCTGAG 
CGGCTGTCCT TGGTGGCAGG GGCCTCAGGG GGGGCTTCTC CTGTCGCCTT CACCCCCCGA 
GGAGGGCCCA GCCCTCCTGG CCACAGCCCG GGCCCTCCAA GAACTTTCCC AAGTGCCCCG 
CCCCGGGCCT CTGGCTCCCA CGGATCCCTG CTCCTGCCCC CTGCATCCAG CCCCCCGCCG 
CCCCAGGTCC CCCAGCGCCG GGGCACGCCG CCCCTCACCC CTGGTCGCCT GACCCAGGAC 
CTCAAGCTCA TCTCAGCCTC TCAGCCGGCC CTCCCCCAGG ACGGGGCGCA GACTCTCCGC 
AGAGCCTCCC CGCACTCCTC TGGGGAGTCG GTGGCAGCCT TCCCGCTCTT TCCCAGAGGC 
GGGGGCGGCA GCGGGGGCAG CGGGGGCCTC GGTCTCCCCG GGAGGCCCTA TGGTGCCGTC 
CCTGGCCAGC ACGTCACTCT GCCTCGGAAG ACATCCTCAG GTTCTTTGCC ACCCCCTCTT 
TCTTTATTTG GGGCAAGAGC CACTTCTTCT GGGGGGCCCC CTCTGACTGC TGCTCCCCAG 
AGGGAACCTG GGGCCAGGTC TGAGCCGGTG CGCTCCAAAC TGCCGTCCAA CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN4 ( XM_002690493.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690539.2 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219814
Clone ID Related Accession (Same CDS sequence)	XM_002690493.5
Accession Version	XM_002690493.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690493.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGGCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690539.2
CDS	1..3606
Translation	MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTEPRGAAHGAADGEGSARGAGKSSTNGDCRRFRGSLASLGSRGGGGAGAGGGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERSGASAQPAASPPPPPPQQPPQPVPASCEQTSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGGLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGVGGSSPAPTPSTAAAAATAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQASQPPPPLPGARGGLGLAEPFLPPPPSARSPSSSPGQLGQPPGELSPGLAAGPPSTPETPPRQPERLSLVAGASGGASPVAFTPRGGPSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFPLFPRGGGGSGGSGGLGLPGRPYGAVPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAAPQREPGARSEPVRSKLPSNL

Target ORF information:

RefSeq Version	XM_002690493.5
Organism	Bos taurus(cattle)
Definition	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690493.5

ORF Insert Sequence:

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGGCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN4 ( XM_002700513.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700559.2 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.

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CloneID	OBa23035
Clone ID Related Accession (Same CDS sequence)	XM_002700513.4 , XM_002690493.4
Accession Version	XM_002700513.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700513.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGNCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700559.2
CDS	136..3741
Translation	MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTEPRGAAHGAADGEGSARGAGKSSTNGDCRRFRGSLASLGSRGGGGAGAGGGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERSGASAQPAASPPPPPPQQPPQPVPASCEQTSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGGLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGVGGSSPAPTPSTAAAAATAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQnullSQPPPPLPGARGGLGLAEPFLPPPPSARSPSSSPGQLGQPPGELSPGLAAGPPSTPETPPRQPERLSLVAGASGGASPVAFTPRGGPSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFPLFPRGGGGSGGSGGLGLPGRPYGAVPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAAPQREPGARSEPVRSKLPSNL

Target ORF information:

RefSeq Version	XM_002700513.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700513.4

ORF Insert Sequence:

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGNCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN4 ( XM_002690493.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690539.1 Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.

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CloneID	OBa23035
Clone ID Related Accession (Same CDS sequence)	XM_002700513.4 , XM_002690493.4
Accession Version	XM_002690493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	LOW QUALITY PROTEIN: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690493.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGNCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690539.1
CDS	136..3741
Translation	MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTEPRGAAHGAADGEGSARGAGKSSTNGDCRRFRGSLASLGSRGGGGAGAGGGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERSGASAQPAASPPPPPPQQPPQPVPASCEQTSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGGLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGVGGSSPAPTPSTAAAAATAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQnullSQPPPPLPGARGGLGLAEPFLPPPPSARSPSSSPGQLGQPPGELSPGLAAGPPSTPETPPRQPERLSLVAGASGGASPVAFTPRGGPSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFPLFPRGGGGSGGSGGLGLPGRPYGAVPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAAPQREPGARSEPVRSKLPSNL

Target ORF information:

RefSeq Version	XM_002690493.4
Organism	Bos taurus(cattle)
Definition	Bos taurus hyperpolarization activated cyclic nucleotide gated potassium channel 4 (HCN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690493.4

ORF Insert Sequence:

ATGGACAAGC TGCCGCCGTC CATGCGCAAG CGACTCTACA GCCTTCCGCA GCAGGTGGGG 
GCCAAGGCGT GGATCATGGA CGAGGAAGAG GACGCCGAGG AGGAGGGGGC TGGGGGCCGC 
CAGGACCCCA GCCGCAGGAG CATCCGGCTG CGGCCGCTGC CTTCGCCTTC ACCCTCGGCT 
GCCGCGGGCG GCACGGAGCC CCGGGGCGCG GCCCACGGGG CGGCAGACGG CGAGGGGTCA 
GCCCGCGGCG CGGGCAAGTC CAGCACGAAC GGCGACTGCA GGCGCTTCCG CGGGAGCCTG 
GCATCGCTCG GCAGCCGGGG CGGCGGCGGC GCGGGAGCGG GGGGCGGCAG CAGTCACGGG 
CACCTGCATG ACTCCGCGGA GGAGCGGCGG CTCATCGCCG AGGGCGACGC GTCCCCCGGT 
GAGGACAGGA CGCCCCCAGG CCTGGCGGCC GAACCCGAGC GCTCCGGCGC CTCGGCGCAA 
CCTGCAGCCT CGCCGCCGCC GCCGCCGCCC CAGCAGCCGC CGCAGCCGGT CCCCGCCTCC 
TGCGAGCAGA CCTCGGTGGA CACCGCGATC AAAGTGGAGG GAGGCGCGGC CGCTGGAGAC 
CAGATCCTCC CTGAGGCCGA GGTGCGCCTG GGCCAGGCCG GCTTTATGCA GCGCCAGTTC 
GGGGCCATGC TCCAGCCCGG GGTCAACAAA TTCTCCCTGA GAATGTTCGG CAGCCAGAAG 
GCGGTAGAAC GCGAGCAGGA GAGGGTTAAG TCGGCCGGGT TTTGGATTAT CCACCCCTAC 
AGCGACTTCA GATTTTACTG GGACCTGACC ATGCTGCTGC TGATGGTAGG AAACCTGATC 
ATCATTCCTG TGGGCATCAC CTTCTTCAAG GATGAGAACA CCACACCATG GATTGTCTTC 
AACGTGGTGT CGGACACGTT CTTCCTCATC GACTTGGTCC TCAACTTCCG CACAGGGATC 
GTGGTGGAGG ACAACACAGA GATCATCCTT GACCCGCAGC GGATTAAGAT GAAGTACCTA 
AAGAGCTGGT TCGTGGTGGA TTTCATCTCC TCCATCCCTG TGGACTACAT CTTCCTCATT 
GTGGAGACAC GCATCGACTC GGAGGTCTAC AAGACTGCCC GGGCTCTGCG CATTGTCCGG 
TTCACCAAGA TCCTCAGCCT CCTGCGCCTC TTGCGTCTCT CCCGCCTCAT TCGATATATT 
CACCAGTGGG AAGAGATCTT TCACATGACC TACGACCTGG CGAGTGCTGT GGTGCGCATC 
GTCAACCTCA TTGGCATGAT GCTGCTGCTC TGCCACTGGG ACGGCTGTCT GCAGTTCCTG 
GTGCCCATGC TGCAGGACTT CCCCGACGAC TGCTGGGTGT CCATCAACAA TATGGTGAAC 
AACTCCTGGG GAAAGCAGTA CTCCTACGCC CTCTTCAAGG CCATGAGCCA TATGCTGTGC 
ATCGGGTATG GCCGGCAGGC CCCGGTGGGC ATGTCGGACG TCTGGCTCAC CATGCTCAGC 
ATGATTGTGG GTGCCACCTG CTACGCCATG TTCATCGGCC ATGCCACCGC CCTCATCCAG 
TCCCTGGACT CCTCCCGGCG CCAGTACCAG GAGAAGTACA AGCAGGTGGA GCAGTACATG 
TCCTTCCACA AGCTCCCGCC CGACACCCGC CAGCGCATCC ATGACTACTA CGAGCACCGC 
TACCAGGGCA AGATGTTCGA CGAGGAGAGC ATCCTGGGCG AGCTGAGCGA GCCACTGCGG 
GAGGAGATCA TCAACTTTAA CTGCAGAAAG CTGGTGGCCT CCATGCCACT GTTTGCCAAC 
GCGGATCCCA ACTTTGTGAC CTCCATGCTG ACCAAGCTGC GCTTCGAGGT CTTCCAGCCT 
GGGGACTACA TCATCCGCGA AGGCACCATT GGCAAGAAGA TGTACTTCAT TCAGCACGGG 
GTGGTCAGCG TGCTCACCAA GGGCAACAAG GAGACCAAGC TGGCAGATGG CTCTTATTTT 
GGAGAAATCT GCCTGCTGAC GCGGGGCCGA CGCACAGCCA GCGTGAGGGC TGACACCTAC 
TGCCGCCTCT ACTCGCTGAG CGTGGACAAC TTCAATGAGG TGCTGGAGGA GTACCCCATG 
ATGCGGCGGG CCTTCGAGAC GGTGGCGCTG GACCGCCTGG ACCGCATCGG CAAGAAAAAC 
TCCATCCTCC TCCACAAAGT CCAGCACGAC CTCAACTCAG GCGTCTTCAA CTACCAGGAA 
AACGAGATCA TCCAGCAGAT CGTGCAGCAC GACAGGGAGA TGGCGCACTG CGCGCATCGC 
GTCCAGGCCG CCGCCTCCGC CACGCCGACC CCCACGCCGG TCATCTGGAC CCCGCTGATC 
CAGGCGCCGC TGCAGGCCGC CGCCGCCACA ACCTCCGTGG CCATCGCCCT CACTCACCAC 
CCGCGCCTGC CGGCTGCCAT CTTCCGGCCT CCCCCGGGGC CCGGGCTCGG CGGCCTCGGG 
GCCGGGCAGA CGCCCCGGCA CCTGAAGCGG CTGCAGTCTC TGATCCCATC GGCGCTGGGC 
TCCGCCTCGC CCGCCAGCAG CCCGTCCCAG GTGGACACGC CGTCTTCCTC CTCCTTTCAC 
ATCCAACAAC TGGCTGGATT CTCCGCGCCG GCCGGTCTGA GCCCGCTCCT CCCCTCGTCC 
AGCTCCTCCC CGCCGCCAGG GGTCGGCGGC TCCTCCCCAG CGCCCACGCC TTCCACCGCC 
GCCGCCGCCG CCACCGCCGG GTTCGGCCAC TTCCACAAGG CGCTGGGGGG CTCCCTGTCC 
TCGTCCGACT CGCCCCTGCT CACCCCGCTG CAGCCGGGCG CCCGCTCCCC GCAGNCCAGC 
CAGCCGCCGC CCCCGCTGCC GGGGGCCCGG GGAGGCCTGG GACTCGCGGA GCCCTTCCTG 
CCGCCCCCGC CGTCGGCCAG GTCCCCGTCG TCCAGCCCCG GGCAGCTGGG CCAGCCTCCC 
GGGGAGCTGT CCCCCGGGCT GGCCGCCGGC CCGCCGAGTA CGCCAGAGAC ACCCCCGCGG 
CAGCCTGAGC GGCTGTCCTT GGTGGCAGGG GCCTCAGGGG GGGCTTCTCC TGTCGCCTTC 
ACCCCCCGAG GAGGGCCCAG CCCTCCTGGC CACAGCCCGG GCCCTCCAAG AACTTTCCCA 
AGTGCCCCGC CCCGGGCCTC TGGCTCCCAC GGATCCCTGC TCCTGCCCCC TGCATCCAGC 
CCCCCGCCGC CCCAGGTCCC CCAGCGCCGG GGCACGCCGC CCCTCACCCC TGGTCGCCTG 
ACCCAGGACC TCAAGCTCAT CTCAGCCTCT CAGCCGGCCC TCCCCCAGGA CGGGGCGCAG 
ACTCTCCGCA GAGCCTCCCC GCACTCCTCT GGGGAGTCGG TGGCAGCCTT CCCGCTCTTT 
CCCAGAGGCG GGGGCGGCAG CGGGGGCAGC GGGGGCCTCG GTCTCCCCGG GAGGCCCTAT 
GGTGCCGTCC CTGGCCAGCA CGTCACTCTG CCTCGGAAGA CATCCTCAGG TTCTTTGCCA 
CCCCCTCTTT CTTTATTTGG GGCAAGAGCC ACTTCTTCTG GGGGGCCCCC TCTGACTGCT 
GCTCCCCAGA GGGAACCTGG GGCCAGGTCT GAGCCGGTGC GCTCCAAACT GCCGTCCAAC 
CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HCN4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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