FREM3 cDNA ORF clone, Bos taurus(cattle)

The following FREM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa93132 XM_015467026.1
Latest version!
Bos taurus FRAS1-related extracellular matrix protein 3 (LOC781161), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa93132 XM_015475386.1
Latest version!
Bos taurus FRAS1-related extracellular matrix protein 3 (LOC781161), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa224487 XM_024977562.1
Latest version!
Bos taurus FRAS1 related extracellular matrix 3 (FREM3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa93132
    Clone ID Related Accession (Same CDS sequence) XM_015475386.1 , XM_015467026.1
    Accession Version XM_015475386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104447.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCAACTG CTGAAATTAT GGACCCTGAA GATGGTTCTG CCATAGGCAC CCTTTCTTCT 
    TCAGGGTTAT CTTTCTCTGA CTACATCTCA CGTCCAAAAG ACCACACCAG CAGCCTCCAT
    TTTGACAAGG ATGAGGCTGA GAACACCTGC CAAGTCCTGA TCATCAATGA CTCTCTTTAT
    GAAGAGGAGG AATCCTTCAG CGTTTCACTG AGCCTGCCAA AGGGAGGGCA ACTGGGGGCC
    AAATTCCCCA CCGCCAAGGT GATAATTCTG GCTGACTGTG ATGATGAGCC TGCCCTGCAC
    TTTGGAGATG CTGAATATCA CGTTGATCAA AGTGCTGGCT CTGTGGAGGT TTGTGTCCGG
    AGGACAGGCA CGGACCTTTC CCAAGCGTCC TCTGTCACCG TGCGCTCCAA GAGAAGAGAG
    CTGGCCTCAG CAGAAGCTGG ATTAGACTAC ACTGGCATCA GCCAAAAACT GAACTTTGCT
    CCAGGAGTGC GTGTTCAGAT GTTCCGAGTG ACAATCCTTG CTGACCTGGG ACAGCCCATC
    TTTGAGAGTT CAGAGCGATT TGAATTACTT CTTCAGATGC CGCTGGGTGC AGCACTTGGA
    GAGCCCGATA AAACCATTTT CATCAATGAC ACGGTCACTG ACTATAAGCA GAGTGCTTGT
    CCTCCTTTTG GTTGTTTAAG AGAGAGGAAG CAGCCAAAAC AGCTCTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330872.1
    CDS348..1058
    Translation

    Target ORF information:

    RefSeq Version XM_015475386.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1-related extracellular matrix protein 3 (LOC781161), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCAACTG CTGAAATTAT GGACCCTGAA GATGGTTCTG CCATAGGCAC CCTTTCTTCT 
    TCAGGGTTAT CTTTCTCTGA CTACATCTCA CGTCCAAAAG ACCACACCAG CAGCCTCCAT
    TTTGACAAGG ATGAGGCTGA GAACACCTGC CAAGTCCTGA TCATCAATGA CTCTCTTTAT
    GAAGAGGAGG AATCCTTCAG CGTTTCACTG AGCCTGCCAA AGGGAGGGCA ACTGGGGGCC
    AAATTCCCCA CCGCCAAGGT GATAATTCTG GCTGACTGTG ATGATGAGCC TGCCCTGCAC
    TTTGGAGATG CTGAATATCA CGTTGATCAA AGTGCTGGCT CTGTGGAGGT TTGTGTCCGG
    AGGACAGGCA CGGACCTTTC CCAAGCGTCC TCTGTCACCG TGCGCTCCAA GAGAAGAGAG
    CTGGCCTCAG CAGAAGCTGG ATTAGACTAC ACTGGCATCA GCCAAAAACT GAACTTTGCT
    CCAGGAGTGC GTGTTCAGAT GTTCCGAGTG ACAATCCTTG CTGACCTGGG ACAGCCCATC
    TTTGAGAGTT CAGAGCGATT TGAATTACTT CTTCAGATGC CGCTGGGTGC AGCACTTGGA
    GAGCCCGATA AAACCATTTT CATCAATGAC ACGGTCACTG ACTATAAGCA GAGTGCTTGT
    CCTCCTTTTG GTTGTTTAAG AGAGAGGAAG CAGCCAAAAC AGCTCTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93132
    Clone ID Related Accession (Same CDS sequence) XM_015475386.1 , XM_015467026.1
    Accession Version XM_015467026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FRAS1-related extracellular matrix protein 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646344.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCAACTG CTGAAATTAT GGACCCTGAA GATGGTTCTG CCATAGGCAC CCTTTCTTCT 
    TCAGGGTTAT CTTTCTCTGA CTACATCTCA CGTCCAAAAG ACCACACCAG CAGCCTCCAT
    TTTGACAAGG ATGAGGCTGA GAACACCTGC CAAGTCCTGA TCATCAATGA CTCTCTTTAT
    GAAGAGGAGG AATCCTTCAG CGTTTCACTG AGCCTGCCAA AGGGAGGGCA ACTGGGGGCC
    AAATTCCCCA CCGCCAAGGT GATAATTCTG GCTGACTGTG ATGATGAGCC TGCCCTGCAC
    TTTGGAGATG CTGAATATCA CGTTGATCAA AGTGCTGGCT CTGTGGAGGT TTGTGTCCGG
    AGGACAGGCA CGGACCTTTC CCAAGCGTCC TCTGTCACCG TGCGCTCCAA GAGAAGAGAG
    CTGGCCTCAG CAGAAGCTGG ATTAGACTAC ACTGGCATCA GCCAAAAACT GAACTTTGCT
    CCAGGAGTGC GTGTTCAGAT GTTCCGAGTG ACAATCCTTG CTGACCTGGG ACAGCCCATC
    TTTGAGAGTT CAGAGCGATT TGAATTACTT CTTCAGATGC CGCTGGGTGC AGCACTTGGA
    GAGCCCGATA AAACCATTTT CATCAATGAC ACGGTCACTG ACTATAAGCA GAGTGCTTGT
    CCTCCTTTTG GTTGTTTAAG AGAGAGGAAG CAGCCAAAAC AGCTCTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322512.1
    CDS348..1058
    Translation

    Target ORF information:

    RefSeq Version XM_015467026.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1-related extracellular matrix protein 3 (LOC781161), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCAACTG CTGAAATTAT GGACCCTGAA GATGGTTCTG CCATAGGCAC CCTTTCTTCT 
    TCAGGGTTAT CTTTCTCTGA CTACATCTCA CGTCCAAAAG ACCACACCAG CAGCCTCCAT
    TTTGACAAGG ATGAGGCTGA GAACACCTGC CAAGTCCTGA TCATCAATGA CTCTCTTTAT
    GAAGAGGAGG AATCCTTCAG CGTTTCACTG AGCCTGCCAA AGGGAGGGCA ACTGGGGGCC
    AAATTCCCCA CCGCCAAGGT GATAATTCTG GCTGACTGTG ATGATGAGCC TGCCCTGCAC
    TTTGGAGATG CTGAATATCA CGTTGATCAA AGTGCTGGCT CTGTGGAGGT TTGTGTCCGG
    AGGACAGGCA CGGACCTTTC CCAAGCGTCC TCTGTCACCG TGCGCTCCAA GAGAAGAGAG
    CTGGCCTCAG CAGAAGCTGG ATTAGACTAC ACTGGCATCA GCCAAAAACT GAACTTTGCT
    CCAGGAGTGC GTGTTCAGAT GTTCCGAGTG ACAATCCTTG CTGACCTGGG ACAGCCCATC
    TTTGAGAGTT CAGAGCGATT TGAATTACTT CTTCAGATGC CGCTGGGTGC AGCACTTGGA
    GAGCCCGATA AAACCATTTT CATCAATGAC ACGGTCACTG ACTATAAGCA GAGTGCTTGT
    CCTCCTTTTG GTTGTTTAAG AGAGAGGAAG CAGCCAAAAC AGCTCTCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224487
    Clone ID Related Accession (Same CDS sequence) XM_024977562.1
    Accession Version XM_024977562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: FRAS1-related extracellular matrix protein 3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 5 indels internal stop codons :: corrected 3 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTGGCA TTTCTCCGCG CCCTGCTGGG ACGCCCCGGC AGCTTGCCGC CGCGCTCGCC 
    TATCTGCTCC TGAGCTGCTC TGCCCTACAG GGACAGACAC CCTCGCCTGA GGCTGAGCCC
    CCTCTGGTGC CCTACCTGCC CATCTGGGGC GCCCTCGGCC GCCTCATTCC CGATGGCCCC
    AGCATCCTCC TTACCAACCC CGGGCTCCGG GTACCCGTGG GCCGCTCGCT TTGGCTCGAC
    CCGCTCCGGG ACCTGGTGAT TCTAGTGCAG CCGNGGGACC GGTGCGAGGT GACGGTGTTG
    GACGCCCTGC CGCCGCGCCA CAGCGCGCTC TCCCCGCGCG GCTTCCCCTG CGCCTTCGGG
    CCCCGCCAGG TCCGCTACAC TCACTGGGGC GCCCGCAGCC CCGGACGCGC GCGGGTGAGG
    CTGCAGCTGC GCTACGACGC CGCGACTTCC ACGCTCGCGC TGCCCTTCAT GCTCGCGGTG
    GACGTGGTCT TCCACCGGCT GAAGCTGGTG ACGCGCAGCA GGCCTTTGGC GGTGGAGGAG
    CTGCGCGGCT GGAGCCACGC CATTGACAGG AGAGTGCTGG CCTTTGCTTC CCCGGAGCCC
    GGGACCGCGG CCACCCGCAG GTGCCGGCTC ACCCCTGTTC CCCCCGAGGG TGGCCCACTG
    CCCAAGTATG GGCGTTTGGC GGATGCCGCG GGGGCCCCTC TCCCCAGGGC CAAGGCCATA
    GACTGTGAGG CTTTTATCCG GGCTGGGGTG CGCTACCAGC ACACCTCCAC CACCCCCTCG
    CCCAACCGGG ACTGTATGCC CATGGTGGCG GAGCTGCTAG GGCCAGGGGA CCCAGGAGCT
    GAGTCAGAGG AGTTGCTGCT CCGAGAACAT TTCCAGCTTC TGGTGAGGAT CCTTCAGGGA
    TCCGAGAACA CGGTGCCCAG GCCCAGCCTC GAAGCTCCGA TGATGCTGGA GGTCCATCAG
    TTCCTGCGGA CAGCCCTGAC GCCCGTAGTC CTGGCTGCGG AGGACAAGGA GTCTAATGCC
    GATGACCCGG TCTTCAACAT TCTCAATGCT CCCATCACCA TGCCGGGGCA CCAGGGCTAC
    ATGGTCAGCA CTGACAACCC CCTGGGCCTT TCAGTGTCCT TCAGCCAGCG GGAGCTGCGG
    GAATTGAAAA TTGCTTATCA GCCCCCTACG GAGAAATCGG ATGCAGAGCA CCTCTTCCAA
    CTGGAGATGG AGGTGGTGGA CGCAGATGGT GCCACCTCAG ACCCCTTTGC CTTTATGGTG
    GTCGTGAAAC CCCTGAACAC CCTGGCCCCG GTGGCTACCT ATGACCAGGG ACTACTACTT
    TTTGAAGGTC AGTCAAGGCC CCTCTCCAGC CCCAACAGCC TTCAGATCAG TGATAGTGAT
    CACCTGGGAG AGGTGAAAGT GGCTGCCATG AGGGGCTTGA GACATGGGCA GCTGGTCGTG
    CTTGGGGCGC CAGCAGGGTG CAAGCATTTC ACGCCAACGG ACCTGGCAGC AGGGCGAGTG
    GCATACCAGC ATGATGGCAG CAACACCTAC AGTGACAACA TCTTCTTCCG GATGGAGGAT
    GGGCGCCACC AGGTGNGATT CCTCTTCCCT CTCGCCATCA TCCCTGTTGA TGATGAACCA
    CCAATGGTCA CTGCCGACAC TGGGCTCGCG GTGACAGAAG GGCAGGCAGT CCAGATCTCC
    CCCTTTGTCC TGAGTGCAAT GGATATTGAC TCTGAGGACC TGACCATCCA TTTTGTGCTG
    GAGGACCAGC CGCTGGAAGG AGAAGAGGAA GAACGAAGGG GGGATCTGGC TCCCAGTTCC
    TTCAACTCAA AGCAGCATCT GGAGGAGATG CTGCTGTGGC AGTCGGAACC ACCTCGGGCT
    CCTGAAGATG AAGGCTGGTA TTACGTGGGA AAGGGAGGAC TTTATAAGAA GGTGGTGACT
    GAGTAGCTTC GACAAGACAT AGTGGAAGGG AGACTCTTCT ACCGCCATCT TGGACCCCTT
    GGCCCTCAAT CCATAATGGC CCAGATAACT TTCCATGTGC AGGATGACCA TGACCCACCC
    AATCTATCCA AGCAACATTT CTTGACCATC AGAGTCCAAC CAGTGGATCT GCAGAGTCCA
    GAGCTGTTTC CAGGAACTAC CCTAGAAATG ACCATGCAGC AATACCAACT CACTCACTTC
    CAGAAGAAAT TCCTCCAATA TATTGACCAG GACTCAGACG ACCAGCACCT GTGGTACACC
    CTGCTGATGC CTCCAACTGA CACAGATGAC AACCACCAAG TCCGGGCTGG AGACATTGTG
    CTCACTGATT CACCAGACAC ACCCACTGTG CACTTCACCC AGGCCCAGGT AAATCACCAT
    GAAGTTGCCT ACCAGCCCCT CTGGGAGAAG CTGGGAATTG TACCATGGAT TGTGCAGTTC
    ACCTACCAGG TGGAGGATGC TGCAGGCAAT AGTGTACCTG GCACATTCAC ATTATTCCTG
    AGGCCTGTGC ACAACCAGCC TCCCCAAGTC ACCGACAGAG GCTTTACTGT CTTAGAAGGA
    GAGAGTTTCA GCCTCAGCAG TAATGAGCTG CATGTTTCCG ACCCTGACAC AGACATCAAC
    CAGATTGTCT TCATATTAGT TTGGGGTCCC CAACATGGAC ATTTGCAGTA CTTAAAAAAA
    TGTGTGGTCC CAGGGGAATA TTTCATGCAA GCTGATATTG TTAATGGGAG TATCTCTTAT
    CGGAACCACA GAAACCAGGC GACTAGTGAT ACCTTCCATC TGGAGGTAAG TGACAAGGTG
    CATCACAAAC CCATCACTGT CCAGATGTCT GTGCATCCTG TAGCAGCTGA CAAAAGTCCC
    AGGACCTCTA TTATAGGTAG TGCATTATTA GATGTGTCCA TAGATGTACT AGAAAATACA
    GCCACTGAGA TCACCATGGA CATCATCTAT GGTCTAAAGG ACACAGGCAA CTTGATGCTC
    TCTTTCACTG TGGAAGATAG TCCCAAATTG GGCATTATTC TGGTGAATGG TTTACCCACA
    GAACAGTTCA CCCAAGAGGA CCTCATCAGT GGGACAGTAG TCTATGTTCA TGCGGGAGGT
    GAAGTTGGAT TCCAGAAGCA GCCTGATGCC TTCAGGCTTG CCTTCTCCAA GGATNCTCAT
    CAGCGGATGA TGGGAGACAG CACAGTGGAA GAAGTACAGG TTCAAGTGGT GGTGCTGCCC
    GTCAACAACA TGGCTCCCAA GGTCTTGGTC AGGGAGCCTT ACGTTGTCCA TGAAGGTGGG
    AAGGGTGCCT TGACCTTACA ACATCTCAGC ATTGAAGATG TAGACACTCC CCAAGATGAA
    ATTCTCTGCA CAGCAAGTGG TCAGCCAGTC TCTGGCTACC TGGAAAATAT GACACCAGCT
    CCTGGATCAG AAGAGACCCG AGCTGGTTAT CCCATCAGCG CTTTCTCTAT CAGAGATGTC
    CAGCTAAGAC ATGTCAACTA GGTACAGAGT ATCCACAAGG GGGTAGAACC ACAAAAAGAT
    CAATTCACCT TTTATTGCTC TGATGGCATC AACTTCTCCC CAAATGTCTT CTTCCCTATA
    ATGATCTTAC CCACCAATGA TGAGCAGCCT GAACTTTTTG CCCGTGAGTT TGTGGTACTA
    GAGGAAATAA GCATCATCAT AGACACCCCA CTGCTCAATG GAGCCGATAC TGACCTGCCA
    CCAAATGAGC TTCACTTTCA GCTCACAGCC CTCCCTCGGC ATGGGCGAAT CACACAGCAG
    CTGGCCACAG AGAGCCAGCC CATCCGTAGC TTCACTTTAC AGGAGATTCA GGAAGCCTCC
    ACCATTGTGT ATGAGCACGA TGACTCTGAG ACCACAGAGG ACAGTTTTGA GGTCTGGCTG
    AGTGACAGCA AGCACGCCAC CCACAGGACG GTACCCATTG TGGTGATCCT GGTGGACGAT
    GAAACCCCTC AGCTGACCAT CAACAATGGG CTGGAAGTAG AGACTGGACA CACTGAGGTC
    ATCACCAATC GGGTCCTCAA AGCCACAGAT CTTGACTCAG ATGATAAGAG CCTCAGTTTT
    ATCCTCCGCT CTGGACCTCA AAAAGGGCTT CTACAGCGAC TGAGAAAACC CAGAGGGGAG
    GTGAGGAACA ACCTCACCTT GGGAATGAAC TTTACCCAGG GTGAGATTGA CCAAGGCCTC
    ATCTGTTACA CCCACACAAG CCAAGGACTT CATGACCTTA TCAAGTTTGG TGTGACTGAT
    GGGATTAACC CTTTGCTAGA CTGCTATTTC AACATCACCA TTGGCAGCTT AGACAGGGTC
    TTCTTACCTG AGGTGGTTAG CAAAGGCGTC ACCTTCACAG AAGGTGACAG AGTGACCGTC
    ACCATGGATC TGCTTAGCAC CAATGATGTC AACATCTTTG ATGAACAGCT GCACTTCAGC
    ATCACAAGGG CTCCTAGCCT GAGGCATTTG GAAATCTCTG ACCACCCTGG GGAGCCCATT
    ACCTCTTTCA CTCAGTGGCA GTTGGCTGGC AACAAGATAG TCTATGTTCG CACTTCAGAT
    GATGAGATAA AGATGGACAG CTTTGAGTTT CAAGAGACTG ATGAACATAG TCCCATGTTT
    CAAACCTTCA GGATCTTCAT CACGGGTGTT AATAATAAGA AACCTATCAT GACCATCCAT
    TAACTGATAC TGCAAGAAGG GGAAAGAAAA TGCATCATTC CCTTTGAGTT GGCAGCGGAA
    GACAAGGATA CCCCAGATGA TCTCCTTCTC TTTACCATCA CCCAGGTCCC CATCCATGGC
    AGGATTTTGT ATAATGGCAG TCGTCCTGTG ACCACCTTCA CCCAGCGGGA CCTGAACGAG
    AACTTGGTTT GCTACTGGCA CGATGGCAGT GCGATAGCTG AAGACAGTTT CTCCTTGACT
    GTGACAGATG GCACTCATAC TGATTTCTAT GTCTTCCCAA ACACTGCTGT GGAGACACAC
    AGACCTCAGG TAATGTGGAT TCACGTAAGA AACTCCCTAG ACAACAGGTT CTCCCAGACT
    GCCTTTAACA GAGATGCCCT GGCCTTGGAA CACCTTCACC CTGGACATGT GGGCTTCCTG
    ATTACCAGCA GGTCTCTCAA GACAGAGGAT TGGGACAGTT CCCACAGACT CCAGAAGTAT
    AAAGTAACCA GAGGACCGGT GCATGGCTTC ATTATCAACA CTGGCCTTGG AAATGACAGC
    ACGCAAGTTT TTACACAAGA TGACATTGAT GAGATGAGGA TCTACTACAT CTTGAATGAA
    GGCAGCAAGG CAACTAAAGA CATCTTCTAC TTCTCGGCAG AAGACAATGG AGGAAATAAG
    CTAACAAATC AACCTTTCCG CCTCTCCTGG GCTCGGATTG CATTGGAGAA AGAGTATTAC
    ATTGTGGATG AAGACTCCAC GTTCTTAGAA GTGACCCTGA CACGCAGAGG ATCCCTTGGA
    GAAACATCCT CTATCAGCAT TGTAACCAAA GATGAAACTG CAAAAACAGA TAAAGATTTT
    AAAGGAAACA TCCAGAAACA AGTCCAGTTC AATCCCAGAC AGACTACAGC CACGTGGAAA
    GTGAGAATTC CATCAGACAA TGAATATGAG GCTTCAGAGA CCTTCCAGAT CATTCTGACA
    AACCCTATCA AGGCTGTACT GGAGTTTCCA GAAATGGCAA CTGCTGAAAT TATGGACCCT
    GAAGATGGTT CTGCCATAGG CACCCTTTCT TCTTCAGGGT TATCTTTCTC TGACTACATC
    TCACGTCCAA AAGACCACAC CAGCAGCCTC CATTTTGACA AGGATGAGGC TGAGAACACC
    TGCCAAGTCC TGATCATCAA TGACTCTCTT TATGAAGAGG AGGAATCCTT CAGCGTTTCA
    CTGAGCCTGC CAAAGGGAGG GCAACTGGGG GCCAAATTCC CCACCGCCAA GGTGATAATT
    CTGGCTGACT GTGATGATGA GCCTGCCCTG CACTTTGGAG ATGCTGAATA TCACGTTGAT
    CAAAGTGCTG GCTCTGTGGA GGTTTGTGTC CGGAGGACAG GCACGGACCT TTCCCAAGCG
    TCCTCTGTCA CCGTGCGCTC CAAGAGAAGA GAGCTGGCCT CAGCAGAAGC TGGATTAGAC
    TACACTGGCA TCAGCCAAAA ACTGAACTTT GCTCCAGGAG TGCGTGTTCA GATGTTCCGA
    GTGACAATCC TTGCTGACCT GGGACAGCCC ATCTTTGAGA GTTCAGAGCG ATTTGAATTA
    CTTCTTCAGA TGCCGCTGGG TGCAGCACTT GGAGAGCCCG ATAAAACCAT TTTCATCAAT
    GACACGGTCA CTGACTATAA GCAGAGTGCT TGTCCTCCTT TTGGTTGTTT AAGAGAGAGG
    AAGCAGCCAA AACAGCTCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833330.1
    CDS1759..8082
    Translation

    Target ORF information:

    RefSeq Version XM_024977562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FRAS1 related extracellular matrix 3 (FREM3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTGGCA TTTCTCCGCG CCCTGCTGGG ACGCCCCGGC AGCTTGCCGC CGCGCTCGCC 
    TATCTGCTCC TGAGCTGCTC TGCCCTACAG GGACAGACAC CCTCGCCTGA GGCTGAGCCC
    CCTCTGGTGC CCTACCTGCC CATCTGGGGC GCCCTCGGCC GCCTCATTCC CGATGGCCCC
    AGCATCCTCC TTACCAACCC CGGGCTCCGG GTACCCGTGG GCCGCTCGCT TTGGCTCGAC
    CCGCTCCGGG ACCTGGTGAT TCTAGTGCAG CCGNGGGACC GGTGCGAGGT GACGGTGTTG
    GACGCCCTGC CGCCGCGCCA CAGCGCGCTC TCCCCGCGCG GCTTCCCCTG CGCCTTCGGG
    CCCCGCCAGG TCCGCTACAC TCACTGGGGC GCCCGCAGCC CCGGACGCGC GCGGGTGAGG
    CTGCAGCTGC GCTACGACGC CGCGACTTCC ACGCTCGCGC TGCCCTTCAT GCTCGCGGTG
    GACGTGGTCT TCCACCGGCT GAAGCTGGTG ACGCGCAGCA GGCCTTTGGC GGTGGAGGAG
    CTGCGCGGCT GGAGCCACGC CATTGACAGG AGAGTGCTGG CCTTTGCTTC CCCGGAGCCC
    GGGACCGCGG CCACCCGCAG GTGCCGGCTC ACCCCTGTTC CCCCCGAGGG TGGCCCACTG
    CCCAAGTATG GGCGTTTGGC GGATGCCGCG GGGGCCCCTC TCCCCAGGGC CAAGGCCATA
    GACTGTGAGG CTTTTATCCG GGCTGGGGTG CGCTACCAGC ACACCTCCAC CACCCCCTCG
    CCCAACCGGG ACTGTATGCC CATGGTGGCG GAGCTGCTAG GGCCAGGGGA CCCAGGAGCT
    GAGTCAGAGG AGTTGCTGCT CCGAGAACAT TTCCAGCTTC TGGTGAGGAT CCTTCAGGGA
    TCCGAGAACA CGGTGCCCAG GCCCAGCCTC GAAGCTCCGA TGATGCTGGA GGTCCATCAG
    TTCCTGCGGA CAGCCCTGAC GCCCGTAGTC CTGGCTGCGG AGGACAAGGA GTCTAATGCC
    GATGACCCGG TCTTCAACAT TCTCAATGCT CCCATCACCA TGCCGGGGCA CCAGGGCTAC
    ATGGTCAGCA CTGACAACCC CCTGGGCCTT TCAGTGTCCT TCAGCCAGCG GGAGCTGCGG
    GAATTGAAAA TTGCTTATCA GCCCCCTACG GAGAAATCGG ATGCAGAGCA CCTCTTCCAA
    CTGGAGATGG AGGTGGTGGA CGCAGATGGT GCCACCTCAG ACCCCTTTGC CTTTATGGTG
    GTCGTGAAAC CCCTGAACAC CCTGGCCCCG GTGGCTACCT ATGACCAGGG ACTACTACTT
    TTTGAAGGTC AGTCAAGGCC CCTCTCCAGC CCCAACAGCC TTCAGATCAG TGATAGTGAT
    CACCTGGGAG AGGTGAAAGT GGCTGCCATG AGGGGCTTGA GACATGGGCA GCTGGTCGTG
    CTTGGGGCGC CAGCAGGGTG CAAGCATTTC ACGCCAACGG ACCTGGCAGC AGGGCGAGTG
    GCATACCAGC ATGATGGCAG CAACACCTAC AGTGACAACA TCTTCTTCCG GATGGAGGAT
    GGGCGCCACC AGGTGNGATT CCTCTTCCCT CTCGCCATCA TCCCTGTTGA TGATGAACCA
    CCAATGGTCA CTGCCGACAC TGGGCTCGCG GTGACAGAAG GGCAGGCAGT CCAGATCTCC
    CCCTTTGTCC TGAGTGCAAT GGATATTGAC TCTGAGGACC TGACCATCCA TTTTGTGCTG
    GAGGACCAGC CGCTGGAAGG AGAAGAGGAA GAACGAAGGG GGGATCTGGC TCCCAGTTCC
    TTCAACTCAA AGCAGCATCT GGAGGAGATG CTGCTGTGGC AGTCGGAACC ACCTCGGGCT
    CCTGAAGATG AAGGCTGGTA TTACGTGGGA AAGGGAGGAC TTTATAAGAA GGTGGTGACT
    GAGTAGCTTC GACAAGACAT AGTGGAAGGG AGACTCTTCT ACCGCCATCT TGGACCCCTT
    GGCCCTCAAT CCATAATGGC CCAGATAACT TTCCATGTGC AGGATGACCA TGACCCACCC
    AATCTATCCA AGCAACATTT CTTGACCATC AGAGTCCAAC CAGTGGATCT GCAGAGTCCA
    GAGCTGTTTC CAGGAACTAC CCTAGAAATG ACCATGCAGC AATACCAACT CACTCACTTC
    CAGAAGAAAT TCCTCCAATA TATTGACCAG GACTCAGACG ACCAGCACCT GTGGTACACC
    CTGCTGATGC CTCCAACTGA CACAGATGAC AACCACCAAG TCCGGGCTGG AGACATTGTG
    CTCACTGATT CACCAGACAC ACCCACTGTG CACTTCACCC AGGCCCAGGT AAATCACCAT
    GAAGTTGCCT ACCAGCCCCT CTGGGAGAAG CTGGGAATTG TACCATGGAT TGTGCAGTTC
    ACCTACCAGG TGGAGGATGC TGCAGGCAAT AGTGTACCTG GCACATTCAC ATTATTCCTG
    AGGCCTGTGC ACAACCAGCC TCCCCAAGTC ACCGACAGAG GCTTTACTGT CTTAGAAGGA
    GAGAGTTTCA GCCTCAGCAG TAATGAGCTG CATGTTTCCG ACCCTGACAC AGACATCAAC
    CAGATTGTCT TCATATTAGT TTGGGGTCCC CAACATGGAC ATTTGCAGTA CTTAAAAAAA
    TGTGTGGTCC CAGGGGAATA TTTCATGCAA GCTGATATTG TTAATGGGAG TATCTCTTAT
    CGGAACCACA GAAACCAGGC GACTAGTGAT ACCTTCCATC TGGAGGTAAG TGACAAGGTG
    CATCACAAAC CCATCACTGT CCAGATGTCT GTGCATCCTG TAGCAGCTGA CAAAAGTCCC
    AGGACCTCTA TTATAGGTAG TGCATTATTA GATGTGTCCA TAGATGTACT AGAAAATACA
    GCCACTGAGA TCACCATGGA CATCATCTAT GGTCTAAAGG ACACAGGCAA CTTGATGCTC
    TCTTTCACTG TGGAAGATAG TCCCAAATTG GGCATTATTC TGGTGAATGG TTTACCCACA
    GAACAGTTCA CCCAAGAGGA CCTCATCAGT GGGACAGTAG TCTATGTTCA TGCGGGAGGT
    GAAGTTGGAT TCCAGAAGCA GCCTGATGCC TTCAGGCTTG CCTTCTCCAA GGATNCTCAT
    CAGCGGATGA TGGGAGACAG CACAGTGGAA GAAGTACAGG TTCAAGTGGT GGTGCTGCCC
    GTCAACAACA TGGCTCCCAA GGTCTTGGTC AGGGAGCCTT ACGTTGTCCA TGAAGGTGGG
    AAGGGTGCCT TGACCTTACA ACATCTCAGC ATTGAAGATG TAGACACTCC CCAAGATGAA
    ATTCTCTGCA CAGCAAGTGG TCAGCCAGTC TCTGGCTACC TGGAAAATAT GACACCAGCT
    CCTGGATCAG AAGAGACCCG AGCTGGTTAT CCCATCAGCG CTTTCTCTAT CAGAGATGTC
    CAGCTAAGAC ATGTCAACTA GGTACAGAGT ATCCACAAGG GGGTAGAACC ACAAAAAGAT
    CAATTCACCT TTTATTGCTC TGATGGCATC AACTTCTCCC CAAATGTCTT CTTCCCTATA
    ATGATCTTAC CCACCAATGA TGAGCAGCCT GAACTTTTTG CCCGTGAGTT TGTGGTACTA
    GAGGAAATAA GCATCATCAT AGACACCCCA CTGCTCAATG GAGCCGATAC TGACCTGCCA
    CCAAATGAGC TTCACTTTCA GCTCACAGCC CTCCCTCGGC ATGGGCGAAT CACACAGCAG
    CTGGCCACAG AGAGCCAGCC CATCCGTAGC TTCACTTTAC AGGAGATTCA GGAAGCCTCC
    ACCATTGTGT ATGAGCACGA TGACTCTGAG ACCACAGAGG ACAGTTTTGA GGTCTGGCTG
    AGTGACAGCA AGCACGCCAC CCACAGGACG GTACCCATTG TGGTGATCCT GGTGGACGAT
    GAAACCCCTC AGCTGACCAT CAACAATGGG CTGGAAGTAG AGACTGGACA CACTGAGGTC
    ATCACCAATC GGGTCCTCAA AGCCACAGAT CTTGACTCAG ATGATAAGAG CCTCAGTTTT
    ATCCTCCGCT CTGGACCTCA AAAAGGGCTT CTACAGCGAC TGAGAAAACC CAGAGGGGAG
    GTGAGGAACA ACCTCACCTT GGGAATGAAC TTTACCCAGG GTGAGATTGA CCAAGGCCTC
    ATCTGTTACA CCCACACAAG CCAAGGACTT CATGACCTTA TCAAGTTTGG TGTGACTGAT
    GGGATTAACC CTTTGCTAGA CTGCTATTTC AACATCACCA TTGGCAGCTT AGACAGGGTC
    TTCTTACCTG AGGTGGTTAG CAAAGGCGTC ACCTTCACAG AAGGTGACAG AGTGACCGTC
    ACCATGGATC TGCTTAGCAC CAATGATGTC AACATCTTTG ATGAACAGCT GCACTTCAGC
    ATCACAAGGG CTCCTAGCCT GAGGCATTTG GAAATCTCTG ACCACCCTGG GGAGCCCATT
    ACCTCTTTCA CTCAGTGGCA GTTGGCTGGC AACAAGATAG TCTATGTTCG CACTTCAGAT
    GATGAGATAA AGATGGACAG CTTTGAGTTT CAAGAGACTG ATGAACATAG TCCCATGTTT
    CAAACCTTCA GGATCTTCAT CACGGGTGTT AATAATAAGA AACCTATCAT GACCATCCAT
    TAACTGATAC TGCAAGAAGG GGAAAGAAAA TGCATCATTC CCTTTGAGTT GGCAGCGGAA
    GACAAGGATA CCCCAGATGA TCTCCTTCTC TTTACCATCA CCCAGGTCCC CATCCATGGC
    AGGATTTTGT ATAATGGCAG TCGTCCTGTG ACCACCTTCA CCCAGCGGGA CCTGAACGAG
    AACTTGGTTT GCTACTGGCA CGATGGCAGT GCGATAGCTG AAGACAGTTT CTCCTTGACT
    GTGACAGATG GCACTCATAC TGATTTCTAT GTCTTCCCAA ACACTGCTGT GGAGACACAC
    AGACCTCAGG TAATGTGGAT TCACGTAAGA AACTCCCTAG ACAACAGGTT CTCCCAGACT
    GCCTTTAACA GAGATGCCCT GGCCTTGGAA CACCTTCACC CTGGACATGT GGGCTTCCTG
    ATTACCAGCA GGTCTCTCAA GACAGAGGAT TGGGACAGTT CCCACAGACT CCAGAAGTAT
    AAAGTAACCA GAGGACCGGT GCATGGCTTC ATTATCAACA CTGGCCTTGG AAATGACAGC
    ACGCAAGTTT TTACACAAGA TGACATTGAT GAGATGAGGA TCTACTACAT CTTGAATGAA
    GGCAGCAAGG CAACTAAAGA CATCTTCTAC TTCTCGGCAG AAGACAATGG AGGAAATAAG
    CTAACAAATC AACCTTTCCG CCTCTCCTGG GCTCGGATTG CATTGGAGAA AGAGTATTAC
    ATTGTGGATG AAGACTCCAC GTTCTTAGAA GTGACCCTGA CACGCAGAGG ATCCCTTGGA
    GAAACATCCT CTATCAGCAT TGTAACCAAA GATGAAACTG CAAAAACAGA TAAAGATTTT
    AAAGGAAACA TCCAGAAACA AGTCCAGTTC AATCCCAGAC AGACTACAGC CACGTGGAAA
    GTGAGAATTC CATCAGACAA TGAATATGAG GCTTCAGAGA CCTTCCAGAT CATTCTGACA
    AACCCTATCA AGGCTGTACT GGAGTTTCCA GAAATGGCAA CTGCTGAAAT TATGGACCCT
    GAAGATGGTT CTGCCATAGG CACCCTTTCT TCTTCAGGGT TATCTTTCTC TGACTACATC
    TCACGTCCAA AAGACCACAC CAGCAGCCTC CATTTTGACA AGGATGAGGC TGAGAACACC
    TGCCAAGTCC TGATCATCAA TGACTCTCTT TATGAAGAGG AGGAATCCTT CAGCGTTTCA
    CTGAGCCTGC CAAAGGGAGG GCAACTGGGG GCCAAATTCC CCACCGCCAA GGTGATAATT
    CTGGCTGACT GTGATGATGA GCCTGCCCTG CACTTTGGAG ATGCTGAATA TCACGTTGAT
    CAAAGTGCTG GCTCTGTGGA GGTTTGTGTC CGGAGGACAG GCACGGACCT TTCCCAAGCG
    TCCTCTGTCA CCGTGCGCTC CAAGAGAAGA GAGCTGGCCT CAGCAGAAGC TGGATTAGAC
    TACACTGGCA TCAGCCAAAA ACTGAACTTT GCTCCAGGAG TGCGTGTTCA GATGTTCCGA
    GTGACAATCC TTGCTGACCT GGGACAGCCC ATCTTTGAGA GTTCAGAGCG ATTTGAATTA
    CTTCTTCAGA TGCCGCTGGG TGCAGCACTT GGAGAGCCCG ATAAAACCAT TTTCATCAAT
    GACACGGTCA CTGACTATAA GCAGAGTGCT TGTCCTCCTT TTGGTTGTTT AAGAGAGAGG
    AAGCAGCCAA AACAGCTCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.