Frem3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frem3
Entrez Gene ID	333315
Full Name	Fras1 related extracellular matrix protein 3
Synonyms	Gm795,NV2
General protein information	Preferred Names Fras1 related extracellular matrix protein 3 Names FRAS1-related extracellular matrix protein 3 NV domain containing protein NV2 NV domain-containing protein 2 embryonic blastocoelar extracellular matrix protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001167898.1	NP_001161370.1	FRAS1-related extracellular matrix protein 3 precursor

Pathways from BioSystems

Homologies

	FREM3	XP_001092507.2
Rattus norvegicus (Norway rat)	Frem3	NP_001178628.1
Homo sapiens (human)	FREM3	NP_001161707.1
Pan troglodytes (chimpanzee)	FREM3	XP_517461.3
Canis lupus familiaris (dog)	FREM3	XP_005629502.1
Mus musculus (house mouse)	Frem3	NP_001161370.1
Bos taurus (cattle)	FREM3	XP_002694406.2

Gene Ontology
Bibliography

Related articles in PubMed

Tbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.
Diman NY, Brooks G, Kruithof BP, Elemento O, Seidman JG, Seidman CE, Basson CT, Hatcher CJ
Circulation research115(10)834-44(2014 Oct)

Segmental and restricted localization pattern of Fras1 in the developing meningeal basement membrane in mouse.
Makrygiannis AK, Pavlakis E, Petrou P, Kalogeraki E, Chalepakis G
Histochemistry and cell biology140(5)595-601(2013 Nov)

Expression of Fraser syndrome genes in normal and polycystic murine kidneys.
Kerecuk L, Long DA, Ali Z, Anders C, Kolatsi-Joannou M, Scambler PJ, Woolf AS
Pediatric nephrology (Berlin, Germany)27(6)991-8(2012 Jun)

The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation.
Yates LL, Papakrivopoulou J, Long DA, Goggolidou P, Connolly JO, Woolf AS, Dean CH
Human molecular genetics19(23)4663-76(2010 Dec)

Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice.
Pavlakis E, Makrygiannis AK, Chiotaki R, Chalepakis G
Histochemistry and cell biology130(4)785-93(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report Fras1 up-regulation/Frem down-regulation in nephrons from mice with polycystic kidney disease and Frem2 mutations.
Title: Expression of Fraser syndrome genes in normal and polycystic murine kidneys.

Frem3 was present in a broad range of epithelial basement membranes where Fras1, Frem1 and Frem2 were missing
Title: Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice.

in the absence of Fras1 the basement membrane localization of Frem3 remains unaffected in contrast to Frem1 and Frem2 which are completely abolished from the basement membrane.
Title: Basement membrane localization of Frem3 is independent of the Fras1/Frem1/Frem2 protein complex within the sublamina densa.

Frem3 is a basement membrane protein that is distinct from other 12 CSPG repeats-containing extracellular matrix protein family proteins in its tissue distribution and competence to assemble into the basement membrane.
Title: Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1.

Study reports the immunofluorescence pattern of Frem3 and a comparative analysis of the spatiotemporal localization of all Fras1/Frem proteins during mouse embryonic development.
Title: Spatiotemporal distribution of Fras1/Frem proteins during mouse embryonic development.

The following Frem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16054	NM_001167898.1 Latest version!	Mus musculus Fras1 related extracellular matrix protein 3 (Frem3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frem3 ( NM_001167898.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161370.1 Mus musculus Fras1 related extracellular matrix protein 3 (Frem3), mRNA.

Price & Availability↑

CloneID	OMu16054
Clone ID Related Accession (Same CDS sequence)	NM_001167898.1
Accession Version	NM_001167898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	FRAS1-related extracellular matrix protein 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130675.7 and AC132606.3. On Nov 18, 2009 this sequence version replaced XM_984073.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGAG ATTCTCTGAG CCTTCCTGGG ATGTCCCTGC AGCTGCTAGT GACTCTCACC 
TGCCTGCTTC TGACTTGTGC ACTGAGGGAA AGGGTACCCT TGAGTGAAAA CGTGTCCCAC 
TGTGAGCTTT ACCTGCCTCC CTGGGGAGCG CTTAATGGTG GTAGTCACCA GAACCCTGGC 
ATTCTGATCG CCAACTCCGG GTTCCAAGTG CCACAGGGCC GCTCGGTTTG GCTTAACCCA 
CTGCGGGATC TAGTGATTCG AGTGCAGCGC GGGGATCAGT GCAAGGTGAC AGTGCTGGAC 
TTCCCGCGGC TCCAAGGCGC GCTCTCCCCG CACCAATTTC CCTGTGATTT CGGGGCCCAA 
CAGGTTAAGT ACACTCACTT CGGCTCGCCC AGCTCCACAA GAACCCGGAT ACAGCTGCAG 
GTGCGCTATG ACGCAGGGAA TTCCACGCTG GTGCTGCCCT TCACTCTGCA AGTGAATGTA 
GTCTTCCCCA AGCTTCAGTT GGTGGCGCGC AACAGGCCAC TGAAAGTGTT TAAAGTGCTA 
GGCTGCAGCC ATGCCATCGA CAGGAGAGTG CTAAACTTTG CCTCCAGGAG CAGGTGCAGG 
CTCACCGTGC TTCCTCACCC TGGCGGACCA CTCCCCAAGT ATGGACTCTT GGTGGACACC 
GCTGGGAACC CTCTCTCCAG GGGCCATCTG GCCGACTGTG AGGCTTTTAT CCAGGCTGGC 
GTACGCTACC AGCATACCAC CACACCATCC TCACCCAGCA GGGACTATGT GCCCATGCTA 
GTGGAGCTGC TTGGACCAGA GTCACAAGGT ACTAGGTCAG TGGAGGTGCT GGCCCAGGAG 
TACTTCCAAA TTCAAGTCAG AATCCAGAAG CGAGCCAGGA GCACAGCACC AATACCTAGT 
CTCGCGGCTC TAATGATAGT GGAGGTTGAA CAGTCCTTAC TGACAGCCCT GACACCTGAT 
GTGCTGGCTG CAGAAGACAG CGAGTCAGAC CCTGACGACC TAGTCTTCAA CATTCTCAAT 
GTACCCGAGG CCCCACCTGG ACACCATGGT GGACAGGGTT ATGTGGTGAA CACAGATGAC 
CCCCTGGGCC TTCCAGTCTC TTTCTTTACC CAGAAAGAGC TTCGAGAGCT GAAAATAGCC 
TATCGACCCC CAACGGGGAG CTCAGAAGGG GACCACGTCT TTCAGCTCAA ACTGCAGGTG 
GTGGATGAAG ATGGTGACAC CTCTGAGCCT TTTGCTTTCA TGGTGGTGGT AAAGTCTATG 
AATGTGCTGG CTCCAATAGC TAGCTACAGA GGAGGACTTG TGGTGTTTGA AGGCCAGGCA 
AGGCCCTTGT CTGATGCCCT AAGCTTTCAG GTCAGTGATA AAGATAATTC CAAAGAAATA 
AAAATTGTTG CAGTCAGAGG TTTGCAACAC GGGCAGCTGG TAGTGCATGG GGCACCTGCT 
GGGTGCAAGA ATTTCACACT GGCAGACCTA AGTGCAGGGA GAGTGGTGTA CCAGCATGAT 
GGCAGTGACT CTTACAGTGA CAACATCATC CTCAAGATGG AGGGTGAGCA CCACCGGGTG 
GACTTCCTCT TCCCCATCAC TATCGTGCCC GTGGATGATG CGGCACCAGT GCTCACCGCC 
AACATGGGAC TCTCCGTGAC TGAAGGGCAA GTGGTCCAGA TATCCCCCTT TGTCTTATGT 
GCCACCGATA TTGATTCTGA GGACTCAGCT ATCCTCTTTG TGTTGGAAGA CGAACACCTG 
GAAGGAAAAG AGGAAGAAAC ACATGAAGAT CTGGCCCCTG GTTCCTACAG CTCAAGCCAG 
CACCCAGGCA ACATGCTGCT GAGGCAGGCT GAGCCCCCTT CGTCCCTTCT GTACAGTGAC 
TGGCACTATG TGGAAAAGGA GGGCCTTTAT GAGACGGTAG TGACTGAGTG GCTGCAGAGA 
GACATAATGG AAGGGAGACT GTTCTTCTCC CATCCTGGAC CACATAGCCC ATCTCCAGTG 
GCTCATCTGG CCTTCCATGT GCAGGATGAC CAGGACCCAC CAAACCTCTC CAACCAGCAC 
TTCTTCACCA TCAGCATCCA GCCTGCAGAT ACGTCGCAGC CTCAGCTATC TCCAGAAACG 
ACCCTAGAAA TGACAGTGCA AGGATACCAG CTCACTCCCT TCCAGCAGAA GTACCTCCAG 
TACACTGACC AGAACTCAGA TGAACAGAAC CTGTGGTACA CTCTACTGAC GCGACCAACA 
GACGCTGACA GCAACCATCA AGTCCAGGCT GGAGAGATCG TGCTCACTGA TTCACCAGGT 
AGACCCATCG TTCATTTTAC CCAAACCCAG GTCAATCATC AAAAAATTGC TTATCGACCC 
CCACAGAGGA ATCTTGGCAT TGTACCTCGA GTTGTACAAT TCACCTATAA GGTGGAAGAC 
ACTGCAGGCA ACAGTGTGTC AGGTACATTC ACAGTATTCC TGCAGCCTTT GAACAATCAG 
CCACCAAAAG TCATCAATAG AGGCTTCGCT GTCTTGGAGG GAGATAGCTT TATTCTTGGC 
AGAGGTGAAC TTAATGTTTC AGACCCTGAC ACCAATGATG ATCAAATTTT CTTCATTTTA 
GTTTGGGGTC CCCAGCATGG ACACTTGCAG TACTTTAAAA AATATATGGT TCCAGGAGAA 
TCATTTATGC TAGCTGATGT TATCAATGGG AGCATCTCTT ATCAGAACAG CAGAGACCAG 
ACTGACAGTG ACGTCATCTA CCTAGAGGTA AGTGATGGAG TGCACCATAT ACCCATCACT 
GTCCAGGTTT CTGTGCAGCC CACTGTGGCT GACAGAAGTC CTGGGATTTC TATCACAGGG 
ACTCCAGTAT TGGCTACTTC TCTAACTGTC CTTGAAAATG CAGCTTCTGA GATCACTATG 
GATGTTACTC ATGGCAGAAA GAACACAAAT GATTTGATGC TATCTTTCAT TGTGGAGGAC 
AAGCCTAAAC TGGGCATGAT TCTAGTGAAT GGTTGGCCTG CAGAGCAAGT CACCCAAGAG 
GACCTCCTTG GTGGGGCAGT CAGCTATGTT CACACTAGTG GTGAAATTGG CTTCCAAAGG 
GTGCATGATA CTTTCAGCTT CATCCTCTCC AAAGATGCTT ATCACCGGGC CATGAGAGAC 
AACATACTGG AGAGGGTACT GGTGGAAGTA ACAGTACTGC CCGTGGATAA CATGGCCCCC 
AAGGTCTTGG TAAGAAAGCC CTTCATCGTA TATGAAGGTG GGAAGAACCT CTTGACCCCA 
CAACATGTCA ACATTGAAGA TGTAGACACT TCCAAAGATG AAATCCTTTG CACTCTGACT 
GTACAGCCAT CCTCTGGCTA CCTGGAAAAC TTGGCACCAG CTCCTGGTTC AGAAGTGTCA 
CGGGCTGGCA ACCCCATCAG TGCCTTCTTC GTCAAGGATG TTCGAGTGGG GTGCATCAAT 
TATGTACAGA GTATCCACAA AGGAATAGAA CCAGGAGCAG ATCAATTCAC CTTTTATTGC 
TCTGACGGCA TCAACTTCTC CCCAAAGGTC CTCCTCCCTC TAACCATCTT GCCCACCAAT 
GATGAGCAGC CTCAACTTTT CACCCGTGAG TTTGTGGTAT TAGAGGGGAT GAGCCGGGTC 
ATAGACACCT CACTGGTCAA TGGAGTAGAT GCTGACTTCC CATCAGATAA GCTTCACTTC 
CGACTCACGG TCTTCCCCCA GCATGGACAA ATCACACAGC AGCTGGCCAC AGGCAGCAAG 
CCCATCCACA GCTTTACCTT GCAGGAGATC CAAGAGCCCT CCTCCATCGC CTATGAACAT 
GATGACTCAG AGAGCACGGA GGACAGCTTT GAGGTCTGGC TGAGTGATGG CAGGCACACC 
ACTCACACAA CGGTGCCCAT TGCTGTAATT TTGGTGGATG ATGAGGTTCC TCAGTTGACC 
ATCAATGACG GACTGGAAAT AGAAACTGGA CACTCTGAGA TCATCACAAA TCATATCCTC 
AAAGCCATAG ATCTCGACTC TGATAACAAG AGTCTCAGTT TTGTTCTCCA CTCTGAGCCT 
CAGCAGGGAC TTCTGCAGCG GCTGAGAAAG CCAGGAGGAG ATGCAAAGCA CAACCTCACT 
GTGGGAATGA ATTTCACCCA GGATGAAATA GACAGAGGCC TTATCCATTA TATCCACACA 
GGCCAGGGAG AGGCTGTTGA TCTCAAATTT GATGTGACTG ATGGTGTTAA CACTTTGAGA 
GATCGCTATT TCTATATCAC CATTGACAAC TCAAACAGTG CCGTCCCTGA GATAGTCAGC 
AAAAGAGTCA CCTTGACAGA AGGTAACAGA ATGACCCTCA CCACCGAACT GCTTAACACC 
AGTGACATCC ACAGCCCTGG TGAACAACTT CTCTACAGTA TCACACGAGC TCCTAGCACA 
GGCCACTTGG AAAGCTCTGA CCAGCCTGGG GAGCCCATGG CTTCTTTTAC TCAGCTTCAA 
CTGGCCAGAA ACAAGATATC CTATGTTCAT ATTTCAAATG ATAAAATAAA GTTGGACCAC 
TTTGAGCTTA GAGTGACTGG AGGACACCAC TCTGAGTCCA GAATCTTTAG AATATTTGTC 
ACAGAGCAGG ACAACAAGAA ACCTACCTTG ACTATCCAAG CACTAGCCCT GCAAAGAGGG 
GACAACATAG TGGTCACTCC TTCTCAGCTG ACAGTGGAAG ATGAGGACAC TCCAGCTGAT 
TTTATTCTTT TTACCATCAC ACAGATCCCC ATCCATGGCA GGATTCTATA TAATGGTAGC 
CGTCCTGTGA CCACTTTCAC CAAGAAGGAC CTGACAAAGA GCCTGATTCT CTATTGTCAC 
GATGGCAGTG AGACTAGCAA AGACAGTTTC TCCTTCACTG TGACTGATGG AATCCACACA 
GGCTTCTATG TCTTCCCAGA CACTTCCCTG GAGACACACG TGCCTCAGAC AGTGTGGATC 
CAGATAAGCC CCTTTGACGA CAGGCTCCCA CAGATGGGCA TCAACAGGGG TGCCACAGCC 
TTGAAGCTCC TTCACACTGG ACACTTGGGT TTCCTCATTA CCAACGAGTA TTTACAAGCA 
ACACATCAGG GCGTTCCCCA CAGACTCCTG ACATATAAAG TGACCAGAGG ACCGGAGCAT 
GGCTACATCG TCAATGCTGG CCTTGGAAAT GAGAACACCC ACATGTTCAC TCAAGCTGAC 
ATTGATGAGA TGCGGGTCTA CTACATCCTG AACAAGGGCA GAGGCCGCGC AACAAGAGAT 
ACCTTCTACT TCTCTGTTGA AACCCGTGGA GGAAAGCAGC TAAGAAACCA GCCTTTCCAT 
CTAAACTGGG CTTGGATTTC TTTGGAAAAA GAATACTACA TTGTGGATGA AGACTCCCCA 
TTCTTCGAAG TGACTCTCAC CCGCAGGGGA TACCTTGGAG GCACATCTGT CGTTAGCATT 
GGAACCAAAG GAGACACTGC AGAAGAAAAT AAGGATTTTA AAGGAAAGGC TCCTATGCTG 
GTCCAGTTCA GTCCCGGCCA GTCTACAGCC ACATGGAGGG TAGGACTTAT ACCCGATACC 
AAGTACGAGA CCTCAGAAAC TTTCCAGATC ATTCTCTCTG AACCTGGCGC GGCTGCACTG 
GAGTTTCCAG AGATGGCAAC AGTTGAAATT GTAGATCCTG AAGATGAATC AACAGTTTAC 
ATTCCAGAGG CAGAATACCA ACTGGCAGAA GCTGTTGGGG AGTTTCTGGT TCCTGTGAGA 
CGATCTGGAG ACACGAGCCA GGAGCTCACG GTCATCTGCT CTACTCGACC AGGTTCTGCC 
ACTGGCACAA TTCCTTCCGT GCAGTTATCT TTGTCAGATT ATGTCTCAAG ACCAGAAGAT 
AATACCAGCA TGCTCCACTT TGAAAAGGAT GAGAGCGAGA AGACCTGCCA GGTCCTGATT 
ATCAATGACT CCCTTTATGA GGAGGAAGAG TCCTTCAGCA TTGCATTGAG CCTGCCAACG 
GGAGGGCAGC TGGGAGCCAA ATTCCCCACT GCCAGGGTAA CGATCCTGGC TGACAGAGAG 
GATGAGCCTG CCCTGCACTT CGAATGCTCT GAGTACCATG CTGAGGAAAG TGCTGGCTAC 
GTGGAGGTGG CCGTCTGGAG AAGAGGCACT GGCCTTTCTC AAGCATCTTC TGTCATTGTG 
CGCTCTAGGG GGACAGAGGA ACAGGCAGCA GAAGCTGGAA CAGACTACAT TGGTGTCAGG 
CAACATCTGC ACTTTAGCCC AGGAGTGAGT GTGCAGAGAC TCCGAGTGAC AATCCTTGAT 
GACCTGCCAC AGCCTGCCTT TGAGGGTCCA GAGATGTTTG AATTACTTCT CCAGATGCCT 
ACAGGTGCAG TGATTGGAGA ACCAAATAAG ACCACTGTTA CCATCAATGA CAGTGTCACC 
AACTGTGAAG AGGAGTGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161370.1
CDS	42..6425
Translation	MAGDSLSLPGMSLQLLVTLTCLLLTCALRERVPLSENVSHCELYLPPWGALNGGSHQNPGILIANSGFQVPQGRSVWLNPLRDLVIRVQRGDQCKVTVLDFPRLQGALSPHQFPCDFGAQQVKYTHFGSPSSTRTRIQLQVRYDAGNSTLVLPFTLQVNVVFPKLQLVARNRPLKVFKVLGCSHAIDRRVLNFASRSRCRLTVLPHPGGPLPKYGLLVDTAGNPLSRGHLADCEAFIQAGVRYQHTTTPSSPSRDYVPMLVELLGPESQGTRSVEVLAQEYFQIQVRIQKRARSTAPIPSLAALMIVEVEQSLLTALTPDVLAAEDSESDPDDLVFNILNVPEAPPGHHGGQGYVVNTDDPLGLPVSFFTQKELRELKIAYRPPTGSSEGDHVFQLKLQVVDEDGDTSEPFAFMVVVKSMNVLAPIASYRGGLVVFEGQARPLSDALSFQVSDKDNSKEIKIVAVRGLQHGQLVVHGAPAGCKNFTLADLSAGRVVYQHDGSDSYSDNIILKMEGEHHRVDFLFPITIVPVDDAAPVLTANMGLSVTEGQVVQISPFVLCATDIDSEDSAILFVLEDEHLEGKEEETHEDLAPGSYSSSQHPGNMLLRQAEPPSSLLYSDWHYVEKEGLYETVVTEWLQRDIMEGRLFFSHPGPHSPSPVAHLAFHVQDDQDPPNLSNQHFFTISIQPADTSQPQLSPETTLEMTVQGYQLTPFQQKYLQYTDQNSDEQNLWYTLLTRPTDADSNHQVQAGEIVLTDSPGRPIVHFTQTQVNHQKIAYRPPQRNLGIVPRVVQFTYKVEDTAGNSVSGTFTVFLQPLNNQPPKVINRGFAVLEGDSFILGRGELNVSDPDTNDDQIFFILVWGPQHGHLQYFKKYMVPGESFMLADVINGSISYQNSRDQTDSDVIYLEVSDGVHHIPITVQVSVQPTVADRSPGISITGTPVLATSLTVLENAASEITMDVTHGRKNTNDLMLSFIVEDKPKLGMILVNGWPAEQVTQEDLLGGAVSYVHTSGEIGFQRVHDTFSFILSKDAYHRAMRDNILERVLVEVTVLPVDNMAPKVLVRKPFIVYEGGKNLLTPQHVNIEDVDTSKDEILCTLTVQPSSGYLENLAPAPGSEVSRAGNPISAFFVKDVRVGCINYVQSIHKGIEPGADQFTFYCSDGINFSPKVLLPLTILPTNDEQPQLFTREFVVLEGMSRVIDTSLVNGVDADFPSDKLHFRLTVFPQHGQITQQLATGSKPIHSFTLQEIQEPSSIAYEHDDSESTEDSFEVWLSDGRHTTHTTVPIAVILVDDEVPQLTINDGLEIETGHSEIITNHILKAIDLDSDNKSLSFVLHSEPQQGLLQRLRKPGGDAKHNLTVGMNFTQDEIDRGLIHYIHTGQGEAVDLKFDVTDGVNTLRDRYFYITIDNSNSAVPEIVSKRVTLTEGNRMTLTTELLNTSDIHSPGEQLLYSITRAPSTGHLESSDQPGEPMASFTQLQLARNKISYVHISNDKIKLDHFELRVTGGHHSESRIFRIFVTEQDNKKPTLTIQALALQRGDNIVVTPSQLTVEDEDTPADFILFTITQIPIHGRILYNGSRPVTTFTKKDLTKSLILYCHDGSETSKDSFSFTVTDGIHTGFYVFPDTSLETHVPQTVWIQISPFDDRLPQMGINRGATALKLLHTGHLGFLITNEYLQATHQGVPHRLLTYKVTRGPEHGYIVNAGLGNENTHMFTQADIDEMRVYYILNKGRGRATRDTFYFSVETRGGKQLRNQPFHLNWAWISLEKEYYIVDEDSPFFEVTLTRRGYLGGTSVVSIGTKGDTAEENKDFKGKAPMLVQFSPGQSTATWRVGLIPDTKYETSETFQIILSEPGAAALEFPEMATVEIVDPEDESTVYIPEAEYQLAEAVGEFLVPVRRSGDTSQELTVICSTRPGSATGTIPSVQLSLSDYVSRPEDNTSMLHFEKDESEKTCQVLIINDSLYEEEESFSIALSLPTGGQLGAKFPTARVTILADREDEPALHFECSEYHAEESAGYVEVAVWRRGTGLSQASSVIVRSRGTEEQAAEAGTDYIGVRQHLHFSPGVSVQRLRVTILDDLPQPAFEGPEMFELLLQMPTGAVIGEPNKTTVTINDSVTNCEEECV

Target ORF information:

RefSeq Version	NM_001167898.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Fras1 related extracellular matrix protein 3 (Frem3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167898.1

ORF Insert Sequence:

ATGGCTGGAG ATTCTCTGAG CCTTCCTGGG ATGTCCCTGC AGCTGCTAGT GACTCTCACC 
TGCCTGCTTC TGACTTGTGC ACTGAGGGAA AGGGTACCCT TGAGTGAAAA CGTGTCCCAC 
TGTGAGCTTT ACCTGCCTCC CTGGGGAGCG CTTAATGGTG GTAGTCACCA GAACCCTGGC 
ATTCTGATCG CCAACTCCGG GTTCCAAGTG CCACAGGGCC GCTCGGTTTG GCTTAACCCA 
CTGCGGGATC TAGTGATTCG AGTGCAGCGC GGGGATCAGT GCAAGGTGAC AGTGCTGGAC 
TTCCCGCGGC TCCAAGGCGC GCTCTCCCCG CACCAATTTC CCTGTGATTT CGGGGCCCAA 
CAGGTTAAGT ACACTCACTT CGGCTCGCCC AGCTCCACAA GAACCCGGAT ACAGCTGCAG 
GTGCGCTATG ACGCAGGGAA TTCCACGCTG GTGCTGCCCT TCACTCTGCA AGTGAATGTA 
GTCTTCCCCA AGCTTCAGTT GGTGGCGCGC AACAGGCCAC TGAAAGTGTT TAAAGTGCTA 
GGCTGCAGCC ATGCCATCGA CAGGAGAGTG CTAAACTTTG CCTCCAGGAG CAGGTGCAGG 
CTCACCGTGC TTCCTCACCC TGGCGGACCA CTCCCCAAGT ATGGACTCTT GGTGGACACC 
GCTGGGAACC CTCTCTCCAG GGGCCATCTG GCCGACTGTG AGGCTTTTAT CCAGGCTGGC 
GTACGCTACC AGCATACCAC CACACCATCC TCACCCAGCA GGGACTATGT GCCCATGCTA 
GTGGAGCTGC TTGGACCAGA GTCACAAGGT ACTAGGTCAG TGGAGGTGCT GGCCCAGGAG 
TACTTCCAAA TTCAAGTCAG AATCCAGAAG CGAGCCAGGA GCACAGCACC AATACCTAGT 
CTCGCGGCTC TAATGATAGT GGAGGTTGAA CAGTCCTTAC TGACAGCCCT GACACCTGAT 
GTGCTGGCTG CAGAAGACAG CGAGTCAGAC CCTGACGACC TAGTCTTCAA CATTCTCAAT 
GTACCCGAGG CCCCACCTGG ACACCATGGT GGACAGGGTT ATGTGGTGAA CACAGATGAC 
CCCCTGGGCC TTCCAGTCTC TTTCTTTACC CAGAAAGAGC TTCGAGAGCT GAAAATAGCC 
TATCGACCCC CAACGGGGAG CTCAGAAGGG GACCACGTCT TTCAGCTCAA ACTGCAGGTG 
GTGGATGAAG ATGGTGACAC CTCTGAGCCT TTTGCTTTCA TGGTGGTGGT AAAGTCTATG 
AATGTGCTGG CTCCAATAGC TAGCTACAGA GGAGGACTTG TGGTGTTTGA AGGCCAGGCA 
AGGCCCTTGT CTGATGCCCT AAGCTTTCAG GTCAGTGATA AAGATAATTC CAAAGAAATA 
AAAATTGTTG CAGTCAGAGG TTTGCAACAC GGGCAGCTGG TAGTGCATGG GGCACCTGCT 
GGGTGCAAGA ATTTCACACT GGCAGACCTA AGTGCAGGGA GAGTGGTGTA CCAGCATGAT 
GGCAGTGACT CTTACAGTGA CAACATCATC CTCAAGATGG AGGGTGAGCA CCACCGGGTG 
GACTTCCTCT TCCCCATCAC TATCGTGCCC GTGGATGATG CGGCACCAGT GCTCACCGCC 
AACATGGGAC TCTCCGTGAC TGAAGGGCAA GTGGTCCAGA TATCCCCCTT TGTCTTATGT 
GCCACCGATA TTGATTCTGA GGACTCAGCT ATCCTCTTTG TGTTGGAAGA CGAACACCTG 
GAAGGAAAAG AGGAAGAAAC ACATGAAGAT CTGGCCCCTG GTTCCTACAG CTCAAGCCAG 
CACCCAGGCA ACATGCTGCT GAGGCAGGCT GAGCCCCCTT CGTCCCTTCT GTACAGTGAC 
TGGCACTATG TGGAAAAGGA GGGCCTTTAT GAGACGGTAG TGACTGAGTG GCTGCAGAGA 
GACATAATGG AAGGGAGACT GTTCTTCTCC CATCCTGGAC CACATAGCCC ATCTCCAGTG 
GCTCATCTGG CCTTCCATGT GCAGGATGAC CAGGACCCAC CAAACCTCTC CAACCAGCAC 
TTCTTCACCA TCAGCATCCA GCCTGCAGAT ACGTCGCAGC CTCAGCTATC TCCAGAAACG 
ACCCTAGAAA TGACAGTGCA AGGATACCAG CTCACTCCCT TCCAGCAGAA GTACCTCCAG 
TACACTGACC AGAACTCAGA TGAACAGAAC CTGTGGTACA CTCTACTGAC GCGACCAACA 
GACGCTGACA GCAACCATCA AGTCCAGGCT GGAGAGATCG TGCTCACTGA TTCACCAGGT 
AGACCCATCG TTCATTTTAC CCAAACCCAG GTCAATCATC AAAAAATTGC TTATCGACCC 
CCACAGAGGA ATCTTGGCAT TGTACCTCGA GTTGTACAAT TCACCTATAA GGTGGAAGAC 
ACTGCAGGCA ACAGTGTGTC AGGTACATTC ACAGTATTCC TGCAGCCTTT GAACAATCAG 
CCACCAAAAG TCATCAATAG AGGCTTCGCT GTCTTGGAGG GAGATAGCTT TATTCTTGGC 
AGAGGTGAAC TTAATGTTTC AGACCCTGAC ACCAATGATG ATCAAATTTT CTTCATTTTA 
GTTTGGGGTC CCCAGCATGG ACACTTGCAG TACTTTAAAA AATATATGGT TCCAGGAGAA 
TCATTTATGC TAGCTGATGT TATCAATGGG AGCATCTCTT ATCAGAACAG CAGAGACCAG 
ACTGACAGTG ACGTCATCTA CCTAGAGGTA AGTGATGGAG TGCACCATAT ACCCATCACT 
GTCCAGGTTT CTGTGCAGCC CACTGTGGCT GACAGAAGTC CTGGGATTTC TATCACAGGG 
ACTCCAGTAT TGGCTACTTC TCTAACTGTC CTTGAAAATG CAGCTTCTGA GATCACTATG 
GATGTTACTC ATGGCAGAAA GAACACAAAT GATTTGATGC TATCTTTCAT TGTGGAGGAC 
AAGCCTAAAC TGGGCATGAT TCTAGTGAAT GGTTGGCCTG CAGAGCAAGT CACCCAAGAG 
GACCTCCTTG GTGGGGCAGT CAGCTATGTT CACACTAGTG GTGAAATTGG CTTCCAAAGG 
GTGCATGATA CTTTCAGCTT CATCCTCTCC AAAGATGCTT ATCACCGGGC CATGAGAGAC 
AACATACTGG AGAGGGTACT GGTGGAAGTA ACAGTACTGC CCGTGGATAA CATGGCCCCC 
AAGGTCTTGG TAAGAAAGCC CTTCATCGTA TATGAAGGTG GGAAGAACCT CTTGACCCCA 
CAACATGTCA ACATTGAAGA TGTAGACACT TCCAAAGATG AAATCCTTTG CACTCTGACT 
GTACAGCCAT CCTCTGGCTA CCTGGAAAAC TTGGCACCAG CTCCTGGTTC AGAAGTGTCA 
CGGGCTGGCA ACCCCATCAG TGCCTTCTTC GTCAAGGATG TTCGAGTGGG GTGCATCAAT 
TATGTACAGA GTATCCACAA AGGAATAGAA CCAGGAGCAG ATCAATTCAC CTTTTATTGC 
TCTGACGGCA TCAACTTCTC CCCAAAGGTC CTCCTCCCTC TAACCATCTT GCCCACCAAT 
GATGAGCAGC CTCAACTTTT CACCCGTGAG TTTGTGGTAT TAGAGGGGAT GAGCCGGGTC 
ATAGACACCT CACTGGTCAA TGGAGTAGAT GCTGACTTCC CATCAGATAA GCTTCACTTC 
CGACTCACGG TCTTCCCCCA GCATGGACAA ATCACACAGC AGCTGGCCAC AGGCAGCAAG 
CCCATCCACA GCTTTACCTT GCAGGAGATC CAAGAGCCCT CCTCCATCGC CTATGAACAT 
GATGACTCAG AGAGCACGGA GGACAGCTTT GAGGTCTGGC TGAGTGATGG CAGGCACACC 
ACTCACACAA CGGTGCCCAT TGCTGTAATT TTGGTGGATG ATGAGGTTCC TCAGTTGACC 
ATCAATGACG GACTGGAAAT AGAAACTGGA CACTCTGAGA TCATCACAAA TCATATCCTC 
AAAGCCATAG ATCTCGACTC TGATAACAAG AGTCTCAGTT TTGTTCTCCA CTCTGAGCCT 
CAGCAGGGAC TTCTGCAGCG GCTGAGAAAG CCAGGAGGAG ATGCAAAGCA CAACCTCACT 
GTGGGAATGA ATTTCACCCA GGATGAAATA GACAGAGGCC TTATCCATTA TATCCACACA 
GGCCAGGGAG AGGCTGTTGA TCTCAAATTT GATGTGACTG ATGGTGTTAA CACTTTGAGA 
GATCGCTATT TCTATATCAC CATTGACAAC TCAAACAGTG CCGTCCCTGA GATAGTCAGC 
AAAAGAGTCA CCTTGACAGA AGGTAACAGA ATGACCCTCA CCACCGAACT GCTTAACACC 
AGTGACATCC ACAGCCCTGG TGAACAACTT CTCTACAGTA TCACACGAGC TCCTAGCACA 
GGCCACTTGG AAAGCTCTGA CCAGCCTGGG GAGCCCATGG CTTCTTTTAC TCAGCTTCAA 
CTGGCCAGAA ACAAGATATC CTATGTTCAT ATTTCAAATG ATAAAATAAA GTTGGACCAC 
TTTGAGCTTA GAGTGACTGG AGGACACCAC TCTGAGTCCA GAATCTTTAG AATATTTGTC 
ACAGAGCAGG ACAACAAGAA ACCTACCTTG ACTATCCAAG CACTAGCCCT GCAAAGAGGG 
GACAACATAG TGGTCACTCC TTCTCAGCTG ACAGTGGAAG ATGAGGACAC TCCAGCTGAT 
TTTATTCTTT TTACCATCAC ACAGATCCCC ATCCATGGCA GGATTCTATA TAATGGTAGC 
CGTCCTGTGA CCACTTTCAC CAAGAAGGAC CTGACAAAGA GCCTGATTCT CTATTGTCAC 
GATGGCAGTG AGACTAGCAA AGACAGTTTC TCCTTCACTG TGACTGATGG AATCCACACA 
GGCTTCTATG TCTTCCCAGA CACTTCCCTG GAGACACACG TGCCTCAGAC AGTGTGGATC 
CAGATAAGCC CCTTTGACGA CAGGCTCCCA CAGATGGGCA TCAACAGGGG TGCCACAGCC 
TTGAAGCTCC TTCACACTGG ACACTTGGGT TTCCTCATTA CCAACGAGTA TTTACAAGCA 
ACACATCAGG GCGTTCCCCA CAGACTCCTG ACATATAAAG TGACCAGAGG ACCGGAGCAT 
GGCTACATCG TCAATGCTGG CCTTGGAAAT GAGAACACCC ACATGTTCAC TCAAGCTGAC 
ATTGATGAGA TGCGGGTCTA CTACATCCTG AACAAGGGCA GAGGCCGCGC AACAAGAGAT 
ACCTTCTACT TCTCTGTTGA AACCCGTGGA GGAAAGCAGC TAAGAAACCA GCCTTTCCAT 
CTAAACTGGG CTTGGATTTC TTTGGAAAAA GAATACTACA TTGTGGATGA AGACTCCCCA 
TTCTTCGAAG TGACTCTCAC CCGCAGGGGA TACCTTGGAG GCACATCTGT CGTTAGCATT 
GGAACCAAAG GAGACACTGC AGAAGAAAAT AAGGATTTTA AAGGAAAGGC TCCTATGCTG 
GTCCAGTTCA GTCCCGGCCA GTCTACAGCC ACATGGAGGG TAGGACTTAT ACCCGATACC 
AAGTACGAGA CCTCAGAAAC TTTCCAGATC ATTCTCTCTG AACCTGGCGC GGCTGCACTG 
GAGTTTCCAG AGATGGCAAC AGTTGAAATT GTAGATCCTG AAGATGAATC AACAGTTTAC 
ATTCCAGAGG CAGAATACCA ACTGGCAGAA GCTGTTGGGG AGTTTCTGGT TCCTGTGAGA 
CGATCTGGAG ACACGAGCCA GGAGCTCACG GTCATCTGCT CTACTCGACC AGGTTCTGCC 
ACTGGCACAA TTCCTTCCGT GCAGTTATCT TTGTCAGATT ATGTCTCAAG ACCAGAAGAT 
AATACCAGCA TGCTCCACTT TGAAAAGGAT GAGAGCGAGA AGACCTGCCA GGTCCTGATT 
ATCAATGACT CCCTTTATGA GGAGGAAGAG TCCTTCAGCA TTGCATTGAG CCTGCCAACG 
GGAGGGCAGC TGGGAGCCAA ATTCCCCACT GCCAGGGTAA CGATCCTGGC TGACAGAGAG 
GATGAGCCTG CCCTGCACTT CGAATGCTCT GAGTACCATG CTGAGGAAAG TGCTGGCTAC 
GTGGAGGTGG CCGTCTGGAG AAGAGGCACT GGCCTTTCTC AAGCATCTTC TGTCATTGTG 
CGCTCTAGGG GGACAGAGGA ACAGGCAGCA GAAGCTGGAA CAGACTACAT TGGTGTCAGG 
CAACATCTGC ACTTTAGCCC AGGAGTGAGT GTGCAGAGAC TCCGAGTGAC AATCCTTGAT 
GACCTGCCAC AGCCTGCCTT TGAGGGTCCA GAGATGTTTG AATTACTTCT CCAGATGCCT 
ACAGGTGCAG TGATTGGAGA ACCAAATAAG ACCACTGTTA CCATCAATGA CAGTGTCACC 
AACTGTGAAG AGGAGTGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.
Circulation research115(10)834-44(2014 Oct)
Diman NY,Brooks G,Kruithof BP,Elemento O,Seidman JG,Seidman CE,Basson CT,Hatcher CJ

Segmental and restricted localization pattern of Fras1 in the developing meningeal basement membrane in mouse.
Histochemistry and cell biology140(5)595-601(2013 Nov)
Makrygiannis AK,Pavlakis E,Petrou P,Kalogeraki E,Chalepakis G

Expression of Fraser syndrome genes in normal and polycystic murine kidneys.
Pediatric nephrology (Berlin, Germany)27(6)991-8(2012 Jun)
Kerecuk L,Long DA,Ali Z,Anders C,Kolatsi-Joannou M,Scambler PJ,Woolf AS

The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation.
Human molecular genetics19(23)4663-76(2010 Dec)
Yates LL,Papakrivopoulou J,Long DA,Goggolidou P,Connolly JO,Woolf AS,Dean CH

Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newborn and adult mice.
Histochemistry and cell biology130(4)785-93(2008 Oct)
Pavlakis E,Makrygiannis AK,Chiotaki R,Chalepakis G

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Frem3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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