SHANK2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015461386.1	XP_015316872.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
XM_015461388.1	XP_015316874.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X5
XM_015470108.1	XP_015325594.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X5
XM_015461384.1	XP_015316870.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_015470105.1	XP_015325591.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_005227382.3	XP_005227439.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
XM_005227382.4	XP_005227439.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_015461388.2	XP_015316874.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
XM_015461387.2	XP_015316873.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
XM_015470106.1	XP_015325592.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
XM_015470107.1	XP_015325593.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X4
XM_015470104.1	XP_015325590.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_015461387.1	XP_015316873.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X4
XM_015461385.1	XP_015316871.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_010826361.2	XP_010824663.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_005197378.3	XP_005197435.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
XM_010821273.2	XP_010819575.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
XM_024987497.1	XP_024843265.1	SH3 and multiple ankyrin repeat domains protein 2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232280	XM_005227382.4 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232281	XM_015461387.2 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232282	XM_015461388.2 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232283	XM_024987497.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa12810	XM_005227382.3 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa12810	XM_005197378.3 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_015461385.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_015461384.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_010826361.2 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_015470105.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_015470104.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa56748	XM_010821273.2 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89183	XM_015461386.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89183	XM_015470106.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89184	XM_015461387.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89184	XM_015470107.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89185	XM_015461388.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa89185	XM_015470108.1 Latest version!	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

SHANK2 ( XM_005227382.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227439.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232280
Clone ID Related Accession (Same CDS sequence)	XM_005227382.4
Accession Version	XM_005227382.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227382.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGTGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTGCAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227439.1
CDS	288..5972
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_005227382.4
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227382.4

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGTGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTGCAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461387.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316873.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

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CloneID	OBa232281
Clone ID Related Accession (Same CDS sequence)	XM_015461387.2
Accession Version	XM_015461387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGTGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTGCAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316873.1
CDS	288..5891
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461387.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461387.2

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGTGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTGCAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461388.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316874.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

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CloneID	OBa232282
Clone ID Related Accession (Same CDS sequence)	XM_015461388.2
Accession Version	XM_015461388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGTGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTGCAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316874.1
CDS	288..5822
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461388.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461388.2

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGTGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTGCAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_024987497.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843265.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

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CloneID	OBa232283
Clone ID Related Accession (Same CDS sequence)	XM_024987497.1
Accession Version	XM_024987497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGTGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTGCAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGATAAA 
CCCGAGGAGA TCATCCCAGC CTCCAAGCCA TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG 
TCCAGGGTGG CCACCATCAG GCAGCGGCCC ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC 
ATGAACTCCG TGTACGAACG CCAGGGCATT GCCGTGATGA CGCCCACTGT CCCCGGGAGC 
CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA GGTACGATGC GAAGGCAGAA GTCAATAGAC 
AGCAGAATCT TTCTATCAGG GATAACGGAG GAGGAGCGGC AGTTTCTGGC CCCGCCAATG 
CTCAAGTTCA CCAGAAGCCT GTCCATGCCG GACACCTCCG AGGACATCCC GCCCCCGCCG 
CAGTCAGTGC CCCCGTCTCC ACCGCCACCC TCCCCCACCG CCTATAACTG CCCCAAGTCT 
CCAACTCCAC GAGTCTACGG GACAATCAAG CCCGCGTTCA ACCAGAACCC CGCCACCAAG 
GTGTCCCCGG CCGCTCGCTC CGACACGGTG GCCACCATCG TGCGGGAGAA GGGGCTCTAC 
CACAGGCGGG AGGTTGACCG CTACTCCCTG GACTCCGAGG ACCTCTACAG TCGCGGCACC 
GGCCCCCAGG CCAACTTCCG CGGCAAGAGG GGCCAGATGC CCGAAAACCC GTACTCCGAG 
GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC 
ATGCTGGTGA AACAGTCCAA CGTGGAGGAC AGCCCCGAGA AGACGTGCTC CATCCCCATC 
CCCACCATCA TCGTCAAGGA GCCCTCCACC AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC 
AGTGTGGAGA CCGACCCACA GGCCTCGGAG CAGCCTGGCC AGCTGCGGCC GGACGACAGC 
CTGACCGTCA GCAGCCCCTT CGCCGCCGCC ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG 
CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC TTCCTGTCCA CGGACCTGGG GGACGAGGAC 
GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC 
TTTGGCGAGG AGGACGGCGC GGAGCCTCTG TCGTCACCGG CGCCCCAGGA GCCTGAGAAC 
CACTTTGTGG GTGCGGGCGA GGCTGGAGCT CAGGGTGAGG CTGGCAGGCT GCCAAACGCC 
CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC CCCGCGGCGA CCCCCCGGAG CATCAACATC 
GCCAGCCCCG AGAACTACGT GCACCCGCTC ACGGGGCGGC TGCTGGACCC CAGCTCGCCG 
CTGGCCCTGG CGCTGTCAGC AAGGGACCGG GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA 
GGGGAGGCCC CCAAGGCCGA CCTCAACAAG CCCCTCTACA TCGACACCAA AATGCGGCCC 
AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG 
CAGGAGACGG AGAACAAGTA CGAGACGGAC CCGGGCAAGG AGCGCAGGGA CGACAAGAAG 
GGCGTGCTCG TCAGCATCGT AGACGCCTCC CAGCAGAAAT CCGCCGGGCT GCTGATGGTC 
CACACGGTGG ACGCGGCCAG GCCGGAGGAC TTCCTGCAGG AAGAGGACGA GAAAGCCGAC 
CCGGAAACGC AGCCAGACCA TCTGCCATCC GAGGCGCCCG AAGGCGCCCC CGAAACCGAA 
GGTGCTTTCC CGACCCCCGC AGGCCCCGAG CCCACGGCCG CCGCCGCCAC CGCGCCCGGC 
AGGACCATCG TGGCGGCGGG CTCCGTGGAG GAGGCGGTGA TCCTGCCGTT CCGCATCCCT 
CCCCCGCCTC TGGCATCCGT GGACCTGGAC GAGGATTTTA TTTTCACAGA GCCATTGCCT 
CCTCCCCTGG AATTTGCCAA TAGTTTTGAT ATCCCCGATG ACCGGGTTGC TTCTGTCCCC 
GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA AACGACACCT CCCAGTCCCC TTCCTTGAAC 
TCCAGCCAGC CAACCAACTC CGCAGACAGT AAGAAGCCGG CTGGTCTCTC AAACTGTCTG 
CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC TTTGACGCCG TCACGGACTC CGGGATCGAG 
GAGGTGGACA GCCGGGGCAG CAGCGACCAC CACCTCGAGA CGACCAGCAC CATCTCCACG 
GTGTCCAGCA TCTCCACCCT GTCCTCCGAG GGCGGGGAGA ACGCGGACAC CTGCACAGTC 
TATGCAGACG GGCAAGCCTT CATGGTTGAC AAACCCCCAG TACCTCCCAA GCCAAAAATG 
AAGCCCATAA TCCACAAAGG CAACACCCTC TACCACGATG CGCTGCTGGA GGAGGACGTG 
GACAGCTTCG TGGTCCCCCC GCCTGCACCT CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC 
CCCACGAAGG TCATCCAGCC AAGGACCTCC AGGCTGTGGG GGGACGTCTC CGAGGTCAAA 
AGCCCGATTC TCTCAGGCCC AAAGGCGAAC GTGATTAGTG AGTTAAACTC AATCCTGCAG 
CAAATGAACC GAGAGAAATC TGCAAAGCCG GGGGAAGGAC TGGACCCAAC GCCAGGAACC 
AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC GAGGTCATAA GCACCGTCTC AGGTACACGG 
AGCACGACAG TCACCTTCAC CGTCCGCCCT GGGACCTCCC AGCCCATCAC CCTGCAGAGC 
CGGCCCCCGG ACTACGAGAG CAGGAACTCA GGACCCAGAC GCGCCCCCAG CCCTGTGGTC 
TCACCAACAG AGCTTAACAA AGAGGTCCTG CCCGCCCCCC TGCCTGCCGC CACTGCGGCC 
CCCTCACCCA CCCTCTCGGA TGTCTTCAGC CTTCCGAGCC AGCCCCCGCC CGGGGACCTG 
TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG TCGCCGTCCC CCTCCATCCT GCAACAGCCA 
ATCTCCAATA AGCCTTTTAC AACTAAGCCT GTCCACCTGT GGACTAAACC CGACGTGGCA 
GACTGGCTGG AAAGTCTAAA CCTGGGTGAA CATAAAGAGG CCTTCATGGA CAATGAGATC 
GACGGCAGCC ACCTCCCAAA CCTCCAGAAA GAAGACCTCA TAGATCTGGG CGTGACTCGA 
GTCGGGCACA GAATGAACAT AGAAAGGGCT CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843265.1
CDS	287..5800
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_024987497.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987497.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT CTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGTGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTGCAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGATAAA 
CCCGAGGAGA TCATCCCAGC CTCCAAGCCA TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG 
TCCAGGGTGG CCACCATCAG GCAGCGGCCC ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC 
ATGAACTCCG TGTACGAACG CCAGGGCATT GCCGTGATGA CGCCCACTGT CCCCGGGAGC 
CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA GGTACGATGC GAAGGCAGAA GTCAATAGAC 
AGCAGAATCT TTCTATCAGG GATAACGGAG GAGGAGCGGC AGTTTCTGGC CCCGCCAATG 
CTCAAGTTCA CCAGAAGCCT GTCCATGCCG GACACCTCCG AGGACATCCC GCCCCCGCCG 
CAGTCAGTGC CCCCGTCTCC ACCGCCACCC TCCCCCACCG CCTATAACTG CCCCAAGTCT 
CCAACTCCAC GAGTCTACGG GACAATCAAG CCCGCGTTCA ACCAGAACCC CGCCACCAAG 
GTGTCCCCGG CCGCTCGCTC CGACACGGTG GCCACCATCG TGCGGGAGAA GGGGCTCTAC 
CACAGGCGGG AGGTTGACCG CTACTCCCTG GACTCCGAGG ACCTCTACAG TCGCGGCACC 
GGCCCCCAGG CCAACTTCCG CGGCAAGAGG GGCCAGATGC CCGAAAACCC GTACTCCGAG 
GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC 
ATGCTGGTGA AACAGTCCAA CGTGGAGGAC AGCCCCGAGA AGACGTGCTC CATCCCCATC 
CCCACCATCA TCGTCAAGGA GCCCTCCACC AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC 
AGTGTGGAGA CCGACCCACA GGCCTCGGAG CAGCCTGGCC AGCTGCGGCC GGACGACAGC 
CTGACCGTCA GCAGCCCCTT CGCCGCCGCC ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG 
CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC TTCCTGTCCA CGGACCTGGG GGACGAGGAC 
GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC 
TTTGGCGAGG AGGACGGCGC GGAGCCTCTG TCGTCACCGG CGCCCCAGGA GCCTGAGAAC 
CACTTTGTGG GTGCGGGCGA GGCTGGAGCT CAGGGTGAGG CTGGCAGGCT GCCAAACGCC 
CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC CCCGCGGCGA CCCCCCGGAG CATCAACATC 
GCCAGCCCCG AGAACTACGT GCACCCGCTC ACGGGGCGGC TGCTGGACCC CAGCTCGCCG 
CTGGCCCTGG CGCTGTCAGC AAGGGACCGG GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA 
GGGGAGGCCC CCAAGGCCGA CCTCAACAAG CCCCTCTACA TCGACACCAA AATGCGGCCC 
AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG 
CAGGAGACGG AGAACAAGTA CGAGACGGAC CCGGGCAAGG AGCGCAGGGA CGACAAGAAG 
GGCGTGCTCG TCAGCATCGT AGACGCCTCC CAGCAGAAAT CCGCCGGGCT GCTGATGGTC 
CACACGGTGG ACGCGGCCAG GCCGGAGGAC TTCCTGCAGG AAGAGGACGA GAAAGCCGAC 
CCGGAAACGC AGCCAGACCA TCTGCCATCC GAGGCGCCCG AAGGCGCCCC CGAAACCGAA 
GGTGCTTTCC CGACCCCCGC AGGCCCCGAG CCCACGGCCG CCGCCGCCAC CGCGCCCGGC 
AGGACCATCG TGGCGGCGGG CTCCGTGGAG GAGGCGGTGA TCCTGCCGTT CCGCATCCCT 
CCCCCGCCTC TGGCATCCGT GGACCTGGAC GAGGATTTTA TTTTCACAGA GCCATTGCCT 
CCTCCCCTGG AATTTGCCAA TAGTTTTGAT ATCCCCGATG ACCGGGTTGC TTCTGTCCCC 
GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA AACGACACCT CCCAGTCCCC TTCCTTGAAC 
TCCAGCCAGC CAACCAACTC CGCAGACAGT AAGAAGCCGG CTGGTCTCTC AAACTGTCTG 
CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC TTTGACGCCG TCACGGACTC CGGGATCGAG 
GAGGTGGACA GCCGGGGCAG CAGCGACCAC CACCTCGAGA CGACCAGCAC CATCTCCACG 
GTGTCCAGCA TCTCCACCCT GTCCTCCGAG GGCGGGGAGA ACGCGGACAC CTGCACAGTC 
TATGCAGACG GGCAAGCCTT CATGGTTGAC AAACCCCCAG TACCTCCCAA GCCAAAAATG 
AAGCCCATAA TCCACAAAGG CAACACCCTC TACCACGATG CGCTGCTGGA GGAGGACGTG 
GACAGCTTCG TGGTCCCCCC GCCTGCACCT CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC 
CCCACGAAGG TCATCCAGCC AAGGACCTCC AGGCTGTGGG GGGACGTCTC CGAGGTCAAA 
AGCCCGATTC TCTCAGGCCC AAAGGCGAAC GTGATTAGTG AGTTAAACTC AATCCTGCAG 
CAAATGAACC GAGAGAAATC TGCAAAGCCG GGGGAAGGAC TGGACCCAAC GCCAGGAACC 
AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC GAGGTCATAA GCACCGTCTC AGGTACACGG 
AGCACGACAG TCACCTTCAC CGTCCGCCCT GGGACCTCCC AGCCCATCAC CCTGCAGAGC 
CGGCCCCCGG ACTACGAGAG CAGGAACTCA GGACCCAGAC GCGCCCCCAG CCCTGTGGTC 
TCACCAACAG AGCTTAACAA AGAGGTCCTG CCCGCCCCCC TGCCTGCCGC CACTGCGGCC 
CCCTCACCCA CCCTCTCGGA TGTCTTCAGC CTTCCGAGCC AGCCCCCGCC CGGGGACCTG 
TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG TCGCCGTCCC CCTCCATCCT GCAACAGCCA 
ATCTCCAATA AGCCTTTTAC AACTAAGCCT GTCCACCTGT GGACTAAACC CGACGTGGCA 
GACTGGCTGG AAAGTCTAAA CCTGGGTGAA CATAAAGAGG CCTTCATGGA CAATGAGATC 
GACGGCAGCC ACCTCCCAAA CCTCCAGAAA GAAGACCTCA TAGATCTGGG CGTGACTCGA 
GTCGGGCACA GAATGAACAT AGAAAGGGCT CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_005227382.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227439.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

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CloneID	OBa12810
Clone ID Related Accession (Same CDS sequence)	XM_005227382.3 , XM_005197378.3
Accession Version	XM_005227382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227439.1
CDS	243..5927
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_005227382.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227382.3

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_005197378.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197435.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

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CloneID	OBa12810
Clone ID Related Accession (Same CDS sequence)	XM_005227382.3 , XM_005197378.3
Accession Version	XM_005197378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197435.1
CDS	243..5927
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_005197378.3
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197378.3

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGATAA ACCCGAGGAG 
ATCATCCCAG CCTCCAAGCC ATCCCGGGCT GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG 
GCCACCATCA GGCAGCGGCC CACCAGCCGG TGCTTCCCCT CCGTCGCGGA CATGAACTCC 
GTGTACGAAC GCCAGGGCAT TGCCGTGATG ACGCCCACTG TCCCCGGGAG CCCAAAGGGC 
CCGTTCCTGG GCCTCCCTCG AGGTACGATG CGAAGGCAGA AGTCAATAGA CAGCAGAATC 
TTTCTATCAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461384.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316870.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_015461384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316870.1
CDS	243..5948
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461384.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461384.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015470105.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325591.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_015470105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325591.1
CDS	208..5913
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015470105.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470105.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015470104.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325590.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_015470104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325590.1
CDS	243..5948
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015470104.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470104.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461385.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316871.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_015461385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316871.1
CDS	208..5913
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461385.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461385.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_010826361.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824663.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_010826361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824663.1
CDS	333..6038
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_010826361.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826361.2

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_010821273.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819575.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

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CloneID	OBa56748
Clone ID Related Accession (Same CDS sequence)	XM_015461384.1 , XM_015470105.1 , XM_015470104.1 , XM_015461385.1 , XM_010826361.2 , XM_010821273.2
Accession Version	XM_010821273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819575.1
CDS	333..6038
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_010821273.2
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821273.2

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG ACAGCAGAAT CTTTCTATCA GGGATAACGG AGGAGGAGCG GCAGTTTCTG 
GCCCCGCCAA TGCTCAAGTT CACCAGAAGC CTGTCCATGC CGGACACCTC CGAGGACATC 
CCGCCCCCGC CGCAGTCAGT GCCCCCGTCT CCACCGCCAC CCTCCCCCAC CGCCTATAAC 
TGCCCCAAGT CTCCAACTCC ACGAGTCTAC GGGACAATCA AGCCCGCGTT CAACCAGAAC 
CCCGCCACCA AGGTGTCCCC GGCCGCTCGC TCCGACACGG TGGCCACCAT CGTGCGGGAG 
AAGGGGCTCT ACCACAGGCG GGAGGTTGAC CGCTACTCCC TGGACTCCGA GGACCTCTAC 
AGTCGCGGCA CCGGCCCCCA GGCCAACTTC CGCGGCAAGA GGGGCCAGAT GCCCGAAAAC 
CCGTACTCCG AGGTGGGGAA GATCGCCAGC AAGGCCGTGT ACGTGCCAGC CAAGCCCGCC 
CGGCGGAAGG GCATGCTGGT GAAACAGTCC AACGTGGAGG ACAGCCCCGA GAAGACGTGC 
TCCATCCCCA TCCCCACCAT CATCGTCAAG GAGCCCTCCA CCAGCAGCAG CGGCAAGAGC 
AGCCAGGGCA GCAGTGTGGA GACCGACCCA CAGGCCTCGG AGCAGCCTGG CCAGCTGCGG 
CCGGACGACA GCCTGACCGT CAGCAGCCCC TTCGCCGCCG CCATCGCCGG GGCTGTCCGC 
GACCGCGAGA AGCGGCTGGA GGCCCGGAGG AACTCCCCAG CCTTCCTGTC CACGGACCTG 
GGGGACGAGG ACGTGGGCCT CGGGCCGCCC GCCCCCCGGG CGCGCCCCTC CAAGTTCCCC 
GAGGAGGGTG GCTTTGGCGA GGAGGACGGC GCGGAGCCTC TGTCGTCACC GGCGCCCCAG 
GAGCCTGAGA ACCACTTTGT GGGTGCGGGC GAGGCTGGAG CTCAGGGTGA GGCTGGCAGG 
CTGCCAAACG CCCCGGGCCA AGCCAAGGTG CCGGAGGGCA GCCCCGCGGC GACCCCCCGG 
AGCATCAACA TCGCCAGCCC CGAGAACTAC GTGCACCCGC TCACGGGGCG GCTGCTGGAC 
CCCAGCTCGC CGCTGGCCCT GGCGCTGTCA GCAAGGGACC GGGCCCTGAA GGAGTCCCAG 
CAGGGCCCCA AAGGGGAGGC CCCCAAGGCC GACCTCAACA AGCCCCTCTA CATCGACACC 
AAAATGCGGC CCAGCGGGGA AGCGGGCTTC CCTCCGGTCG GCAGGCAGAG CGGGCGGGGG 
CCCCTGCGGA GGCAGGAGAC GGAGAACAAG TACGAGACGG ACCCGGGCAA GGAGCGCAGG 
GACGACAAGA AGGGCGTGCT CGTCAGCATC GTAGACGCCT CCCAGCAGAA ATCCGCCGGG 
CTGCTGATGG TCCACACGGT GGACGCGGCC AGGCCGGAGG ACTTCCTGCA GGAAGAGGAC 
GAGAAAGCCG ACCCGGAAAC GCAGCCAGAC CATCTGCCAT CCGAGGCGCC CGAAGGCGCC 
CCCGAAACCG AAGGTGCTTT CCCGACCCCC GCAGGCCCCG AGCCCACGGC CGCCGCCGCC 
ACCGCGCCCG GCAGGACCAT CGTGGCGGCG GGCTCCGTGG AGGAGGCGGT GATCCTGCCG 
TTCCGCATCC CTCCCCCGCC TCTGGCATCC GTGGACCTGG ACGAGGATTT TATTTTCACA 
GAGCCATTGC CTCCTCCCCT GGAATTTGCC AATAGTTTTG ATATCCCCGA TGACCGGGTT 
GCTTCTGTCC CCGCTCTCTC AGACCTGGTC AAGCAGAAAA AAAACGACAC CTCCCAGTCC 
CCTTCCTTGA ACTCCAGCCA GCCAACCAAC TCCGCAGACA GTAAGAAGCC GGCTGGTCTC 
TCAAACTGTC TGCCCGCCTC ATTCCTGCCG CCCCCCGAAA GCTTTGACGC CGTCACGGAC 
TCCGGGATCG AGGAGGTGGA CAGCCGGGGC AGCAGCGACC ACCACCTCGA GACGACCAGC 
ACCATCTCCA CGGTGTCCAG CATCTCCACC CTGTCCTCCG AGGGCGGGGA GAACGCGGAC 
ACCTGCACAG TCTATGCAGA CGGGCAAGCC TTCATGGTTG ACAAACCCCC AGTACCTCCC 
AAGCCAAAAA TGAAGCCCAT AATCCACAAA GGCAACACCC TCTACCACGA TGCGCTGCTG 
GAGGAGGACG TGGACAGCTT CGTGGTCCCC CCGCCTGCAC CTCCGCCGCC GCCTGGCGGG 
GCCCAGCCCG GCCCCACGAA GGTCATCCAG CCAAGGACCT CCAGGCTGTG GGGGGACGTC 
TCCGAGGTCA AAAGCCCGAT TCTCTCAGGC CCAAAGGCGA ACGTGATTAG TGAGTTAAAC 
TCAATCCTGC AGCAAATGAA CCGAGAGAAA TCTGCAAAGC CGGGGGAAGG ACTGGACCCA 
ACGCCAGGAA CCAAGCCCGC CGGCCTCGCC CCCAGAGGCC CCGAGGTCAT AAGCACCGTC 
TCAGGTACAC GGAGCACGAC AGTCACCTTC ACCGTCCGCC CTGGGACCTC CCAGCCCATC 
ACCCTGCAGA GCCGGCCCCC GGACTACGAG AGCAGGAACT CAGGACCCAG ACGCGCCCCC 
AGCCCTGTGG TCTCACCAAC AGAGCTTAAC AAAGAGGTCC TGCCCGCCCC CCTGCCTGCC 
GCCACTGCGG CCCCCTCACC CACCCTCTCG GATGTCTTCA GCCTTCCGAG CCAGCCCCCG 
CCCGGGGACC TGTTTGGCTT GAACCCAGTG GGACGCAGCA GGTCGCCGTC CCCCTCCATC 
CTGCAACAGC CAATCTCCAA TAAGCCTTTT ACAACTAAGC CTGTCCACCT GTGGACTAAA 
CCCGACGTGG CAGACTGGCT GGAAAGTCTA AACCTGGGTG AACATAAAGA GGCCTTCATG 
GACAATGAGA TCGACGGCAG CCACCTCCCA AACCTCCAGA AAGAAGACCT CATAGATCTG 
GGCGTGACTC GAGTCGGGCA CAGAATGAAC ATAGAAAGGG CTCTCAAACA GCTGCTGGAC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461386.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316872.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.

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CloneID	OBa89183
Clone ID Related Accession (Same CDS sequence)	XM_015461386.1 , XM_015470106.1
Accession Version	XM_015461386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316872.1
CDS	243..5927
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461386.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461386.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015470106.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325592.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.

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CloneID	OBa89183
Clone ID Related Accession (Same CDS sequence)	XM_015461386.1 , XM_015470106.1
Accession Version	XM_015470106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325592.1
CDS	243..5927
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015470106.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470106.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGCTCCT CCGCCTCCAA AGCGAGCTCC GACCACGGCC 
CTCACCCTGC GCTCCAAGTC CATGACCTCG GAGCTGGAGG AGCTCGCCTC CGTCCGGAAG 
AAGAAGGATA AACCCGAGGA GATCATCCCA GCCTCCAAGC CATCCCGGGC TGCCGAGAGC 
GTGGCGCTGG AGTCCAGGGT GGCCACCATC AGGCAGCGGC CCACCAGCCG GTGCTTCCCC 
TCCGTCGCGG ACATGAACTC CGTGTACGAA CGCCAGGGCA TTGCCGTGAT GACGCCCACT 
GTCCCCGGGA GCCCAAAGGG CCCGTTCCTG GGCCTCCCTC GAGGTACGAT GCGAAGGCAG 
AAGTCAATAG GGATAACGGA GGAGGAGCGG CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC 
ACCAGAAGCC TGTCCATGCC GGACACCTCC GAGGACATCC CGCCCCCGCC GCAGTCAGTG 
CCCCCGTCTC CACCGCCACC CTCCCCCACC GCCTATAACT GCCCCAAGTC TCCAACTCCA 
CGAGTCTACG GGACAATCAA GCCCGCGTTC AACCAGAACC CCGCCACCAA GGTGTCCCCG 
GCCGCTCGCT CCGACACGGT GGCCACCATC GTGCGGGAGA AGGGGCTCTA CCACAGGCGG 
GAGGTTGACC GCTACTCCCT GGACTCCGAG GACCTCTACA GTCGCGGCAC CGGCCCCCAG 
GCCAACTTCC GCGGCAAGAG GGGCCAGATG CCCGAAAACC CGTACTCCGA GGTGGGGAAG 
ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC AAGCCCGCCC GGCGGAAGGG CATGCTGGTG 
AAACAGTCCA ACGTGGAGGA CAGCCCCGAG AAGACGTGCT CCATCCCCAT CCCCACCATC 
ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG 
ACCGACCCAC AGGCCTCGGA GCAGCCTGGC CAGCTGCGGC CGGACGACAG CCTGACCGTC 
AGCAGCCCCT TCGCCGCCGC CATCGCCGGG GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG 
GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC ACGGACCTGG GGGACGAGGA CGTGGGCCTC 
GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG 
GAGGACGGCG CGGAGCCTCT GTCGTCACCG GCGCCCCAGG AGCCTGAGAA CCACTTTGTG 
GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG GCTGGCAGGC TGCCAAACGC CCCGGGCCAA 
GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG ACCCCCCGGA GCATCAACAT CGCCAGCCCC 
GAGAACTACG TGCACCCGCT CACGGGGCGG CTGCTGGACC CCAGCTCGCC GCTGGCCCTG 
GCGCTGTCAG CAAGGGACCG GGCCCTGAAG GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC 
CCCAAGGCCG ACCTCAACAA GCCCCTCTAC ATCGACACCA AAATGCGGCC CAGCGGGGAA 
GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC GGGCGGGGGC CCCTGCGGAG GCAGGAGACG 
GAGAACAAGT ACGAGACGGA CCCGGGCAAG GAGCGCAGGG ACGACAAGAA GGGCGTGCTC 
GTCAGCATCG TAGACGCCTC CCAGCAGAAA TCCGCCGGGC TGCTGATGGT CCACACGGTG 
GACGCGGCCA GGCCGGAGGA CTTCCTGCAG GAAGAGGACG AGAAAGCCGA CCCGGAAACG 
CAGCCAGACC ATCTGCCATC CGAGGCGCCC GAAGGCGCCC CCGAAACCGA AGGTGCTTTC 
CCGACCCCCG CAGGCCCCGA GCCCACGGCC GCCGCCGCCA CCGCGCCCGG CAGGACCATC 
GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG ATCCTGCCGT TCCGCATCCC TCCCCCGCCT 
CTGGCATCCG TGGACCTGGA CGAGGATTTT ATTTTCACAG AGCCATTGCC TCCTCCCCTG 
GAATTTGCCA ATAGTTTTGA TATCCCCGAT GACCGGGTTG CTTCTGTCCC CGCTCTCTCA 
GACCTGGTCA AGCAGAAAAA AAACGACACC TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG 
CCAACCAACT CCGCAGACAG TAAGAAGCCG GCTGGTCTCT CAAACTGTCT GCCCGCCTCA 
TTCCTGCCGC CCCCCGAAAG CTTTGACGCC GTCACGGACT CCGGGATCGA GGAGGTGGAC 
AGCCGGGGCA GCAGCGACCA CCACCTCGAG ACGACCAGCA CCATCTCCAC GGTGTCCAGC 
ATCTCCACCC TGTCCTCCGA GGGCGGGGAG AACGCGGACA CCTGCACAGT CTATGCAGAC 
GGGCAAGCCT TCATGGTTGA CAAACCCCCA GTACCTCCCA AGCCAAAAAT GAAGCCCATA 
ATCCACAAAG GCAACACCCT CTACCACGAT GCGCTGCTGG AGGAGGACGT GGACAGCTTC 
GTGGTCCCCC CGCCTGCACC TCCGCCGCCG CCTGGCGGGG CCCAGCCCGG CCCCACGAAG 
GTCATCCAGC CAAGGACCTC CAGGCTGTGG GGGGACGTCT CCGAGGTCAA AAGCCCGATT 
CTCTCAGGCC CAAAGGCGAA CGTGATTAGT GAGTTAAACT CAATCCTGCA GCAAATGAAC 
CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA CTGGACCCAA CGCCAGGAAC CAAGCCCGCC 
GGCCTCGCCC CCAGAGGCCC CGAGGTCATA AGCACCGTCT CAGGTACACG GAGCACGACA 
GTCACCTTCA CCGTCCGCCC TGGGACCTCC CAGCCCATCA CCCTGCAGAG CCGGCCCCCG 
GACTACGAGA GCAGGAACTC AGGACCCAGA CGCGCCCCCA GCCCTGTGGT CTCACCAACA 
GAGCTTAACA AAGAGGTCCT GCCCGCCCCC CTGCCTGCCG CCACTGCGGC CCCCTCACCC 
ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC CAGCCCCCGC CCGGGGACCT GTTTGGCTTG 
AACCCAGTGG GACGCAGCAG GTCGCCGTCC CCCTCCATCC TGCAACAGCC AATCTCCAAT 
AAGCCTTTTA CAACTAAGCC TGTCCACCTG TGGACTAAAC CCGACGTGGC AGACTGGCTG 
GAAAGTCTAA ACCTGGGTGA ACATAAAGAG GCCTTCATGG ACAATGAGAT CGACGGCAGC 
CACCTCCCAA ACCTCCAGAA AGAAGACCTC ATAGATCTGG GCGTGACTCG AGTCGGGCAC 
AGAATGAACA TAGAAAGGGC TCTCAAACAG CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015470107.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325593.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.

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CloneID	OBa89184
Clone ID Related Accession (Same CDS sequence)	XM_015470107.1 , XM_015461387.1
Accession Version	XM_015470107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325593.1
CDS	243..5846
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015470107.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470107.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461387.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316873.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.

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CloneID	OBa89184
Clone ID Related Accession (Same CDS sequence)	XM_015470107.1 , XM_015461387.1
Accession Version	XM_015461387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316873.1
CDS	243..5846
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPLVPTRRTVPAAPPLSHRPAGGWAQSCILEEPSGLFPPAPGLHRTCSGEQAHWTSSVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461387.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461387.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTACCCC TCGTGCCAAC CAGGAGGACT 
GTCCCTGCGG CTCCTCCCCT GTCCCATCGC CCTGCCGGCG GCTGGGCCCA GTCTTGCATC 
TTGGAAGAGC CCTCTGGCCT GTTCCCTCCA GCCCCGGGCT TACACCGGAC GTGCAGCGGG 
GAGCAGGCCC ACTGGACCAG CAGCGTGCCC TTCCGAGAGG CTCCGGCGTA CTCCAACCGC 
AGGCGGCGGC CCCCCAGCAC GCTGGCCGCC CCGCGGCTCC TGCTGCGCTC CAACAGCGAT 
AACAACCTCC ACGCCGGCGC GCCCGACTGG GCTGTCTGCG CGGCCACCTC CCGCCTCAGC 
CTCTCGCCCC AGCTGCTGCA GCAGACGCCC GGCAAGGCCG ACGGGGCCGC CAAGACCCTC 
GGGAGCTACA CCCCCGGGCC CCGCAGCCGG TCGCCCTCGC TCAACAGGCT GGGCGGAGCC 
GGCGAGGATG CCAAGAGGCC CCAGCAGCCC TGGCACGTCG GCCCAGCTTT CCCGCCAGGA 
GCCAACAAGG ACGTGCTGTC TGCCTTTGAG TACCCGGGGC CCAGACGGAA GCTGTACAGC 
GCGGTGCCTG GGAGGCTCTT CGTCGTCGTC AAGCCATACC AACCCCAGGT CGATGGCGAG 
ATCCCCCTGC ACCGTGGCGA CAGGGTCAAA GTTCTGAGCA TCGGCGAAGG CGGCTTCTGG 
GAAGGCAGCG CCCGCGGCCA CATCGGATGG TTCCCGGCAG AGTGTGTGGA GGAGGTCCAC 
TGCAAGCCCC GGGACAGCCA GGCAGAAACT CGTGCAGACC GCAGCAAGAA ACTCTTCCGG 
CACTACACAG TTGGCTCCTA CGACAGCTTC GATGCCTCCA GCGACTGCGT TATCGAGGAG 
AAGACCGTGG TCCTGCAGAA GAAAGACAAT GAGGGCTTCG GATTCGTGCT CCGAGGGGCG 
AAAGCGGATA CGCCCATCGA AGAATTCACG CCCACGCCGG CGTTCCCGGC CCTACAGTAC 
CTGGAGTCTG TGGATGAAGG CGGGGTGGCA TGGCAAGCCG GACTGAGGAC CGGGGACTTC 
TTGATTGAGG TGAACAACGA GAACGTCGTC AAGGTTGGCC ACCGGCAGGT CGTGACCATG 
ATCCGCCAGG GAGGGAACCA CCTGGTCCTC AAGGTGGTCA CGGTGACCAG GAGTCTGGAC 
CCGGATGACA CCGCCCGGAA GAAAGATAAA CCCGAGGAGA TCATCCCAGC CTCCAAGCCA 
TCCCGGGCTG CCGAGAGCGT GGCGCTGGAG TCCAGGGTGG CCACCATCAG GCAGCGGCCC 
ACCAGCCGGT GCTTCCCCTC CGTCGCGGAC ATGAACTCCG TGTACGAACG CCAGGGCATT 
GCCGTGATGA CGCCCACTGT CCCCGGGAGC CCAAAGGGCC CGTTCCTGGG CCTCCCTCGA 
GGTACGATGC GAAGGCAGAA GTCAATAGAC AGCAGAATCT TTCTATCAGG GATAACGGAG 
GAGGAGCGGC AGTTTCTGGC CCCGCCAATG CTCAAGTTCA CCAGAAGCCT GTCCATGCCG 
GACACCTCCG AGGACATCCC GCCCCCGCCG CAGTCAGTGC CCCCGTCTCC ACCGCCACCC 
TCCCCCACCG CCTATAACTG CCCCAAGTCT CCAACTCCAC GAGTCTACGG GACAATCAAG 
CCCGCGTTCA ACCAGAACCC CGCCACCAAG GTGTCCCCGG CCGCTCGCTC CGACACGGTG 
GCCACCATCG TGCGGGAGAA GGGGCTCTAC CACAGGCGGG AGGTTGACCG CTACTCCCTG 
GACTCCGAGG ACCTCTACAG TCGCGGCACC GGCCCCCAGG CCAACTTCCG CGGCAAGAGG 
GGCCAGATGC CCGAAAACCC GTACTCCGAG GTGGGGAAGA TCGCCAGCAA GGCCGTGTAC 
GTGCCAGCCA AGCCCGCCCG GCGGAAGGGC ATGCTGGTGA AACAGTCCAA CGTGGAGGAC 
AGCCCCGAGA AGACGTGCTC CATCCCCATC CCCACCATCA TCGTCAAGGA GCCCTCCACC 
AGCAGCAGCG GCAAGAGCAG CCAGGGCAGC AGTGTGGAGA CCGACCCACA GGCCTCGGAG 
CAGCCTGGCC AGCTGCGGCC GGACGACAGC CTGACCGTCA GCAGCCCCTT CGCCGCCGCC 
ATCGCCGGGG CTGTCCGCGA CCGCGAGAAG CGGCTGGAGG CCCGGAGGAA CTCCCCAGCC 
TTCCTGTCCA CGGACCTGGG GGACGAGGAC GTGGGCCTCG GGCCGCCCGC CCCCCGGGCG 
CGCCCCTCCA AGTTCCCCGA GGAGGGTGGC TTTGGCGAGG AGGACGGCGC GGAGCCTCTG 
TCGTCACCGG CGCCCCAGGA GCCTGAGAAC CACTTTGTGG GTGCGGGCGA GGCTGGAGCT 
CAGGGTGAGG CTGGCAGGCT GCCAAACGCC CCGGGCCAAG CCAAGGTGCC GGAGGGCAGC 
CCCGCGGCGA CCCCCCGGAG CATCAACATC GCCAGCCCCG AGAACTACGT GCACCCGCTC 
ACGGGGCGGC TGCTGGACCC CAGCTCGCCG CTGGCCCTGG CGCTGTCAGC AAGGGACCGG 
GCCCTGAAGG AGTCCCAGCA GGGCCCCAAA GGGGAGGCCC CCAAGGCCGA CCTCAACAAG 
CCCCTCTACA TCGACACCAA AATGCGGCCC AGCGGGGAAG CGGGCTTCCC TCCGGTCGGC 
AGGCAGAGCG GGCGGGGGCC CCTGCGGAGG CAGGAGACGG AGAACAAGTA CGAGACGGAC 
CCGGGCAAGG AGCGCAGGGA CGACAAGAAG GGCGTGCTCG TCAGCATCGT AGACGCCTCC 
CAGCAGAAAT CCGCCGGGCT GCTGATGGTC CACACGGTGG ACGCGGCCAG GCCGGAGGAC 
TTCCTGCAGG AAGAGGACGA GAAAGCCGAC CCGGAAACGC AGCCAGACCA TCTGCCATCC 
GAGGCGCCCG AAGGCGCCCC CGAAACCGAA GGTGCTTTCC CGACCCCCGC AGGCCCCGAG 
CCCACGGCCG CCGCCGCCAC CGCGCCCGGC AGGACCATCG TGGCGGCGGG CTCCGTGGAG 
GAGGCGGTGA TCCTGCCGTT CCGCATCCCT CCCCCGCCTC TGGCATCCGT GGACCTGGAC 
GAGGATTTTA TTTTCACAGA GCCATTGCCT CCTCCCCTGG AATTTGCCAA TAGTTTTGAT 
ATCCCCGATG ACCGGGTTGC TTCTGTCCCC GCTCTCTCAG ACCTGGTCAA GCAGAAAAAA 
AACGACACCT CCCAGTCCCC TTCCTTGAAC TCCAGCCAGC CAACCAACTC CGCAGACAGT 
AAGAAGCCGG CTGGTCTCTC AAACTGTCTG CCCGCCTCAT TCCTGCCGCC CCCCGAAAGC 
TTTGACGCCG TCACGGACTC CGGGATCGAG GAGGTGGACA GCCGGGGCAG CAGCGACCAC 
CACCTCGAGA CGACCAGCAC CATCTCCACG GTGTCCAGCA TCTCCACCCT GTCCTCCGAG 
GGCGGGGAGA ACGCGGACAC CTGCACAGTC TATGCAGACG GGCAAGCCTT CATGGTTGAC 
AAACCCCCAG TACCTCCCAA GCCAAAAATG AAGCCCATAA TCCACAAAGG CAACACCCTC 
TACCACGATG CGCTGCTGGA GGAGGACGTG GACAGCTTCG TGGTCCCCCC GCCTGCACCT 
CCGCCGCCGC CTGGCGGGGC CCAGCCCGGC CCCACGAAGG TCATCCAGCC AAGGACCTCC 
AGGCTGTGGG GGGACGTCTC CGAGGTCAAA AGCCCGATTC TCTCAGGCCC AAAGGCGAAC 
GTGATTAGTG AGTTAAACTC AATCCTGCAG CAAATGAACC GAGAGAAATC TGCAAAGCCG 
GGGGAAGGAC TGGACCCAAC GCCAGGAACC AAGCCCGCCG GCCTCGCCCC CAGAGGCCCC 
GAGGTCATAA GCACCGTCTC AGGTACACGG AGCACGACAG TCACCTTCAC CGTCCGCCCT 
GGGACCTCCC AGCCCATCAC CCTGCAGAGC CGGCCCCCGG ACTACGAGAG CAGGAACTCA 
GGACCCAGAC GCGCCCCCAG CCCTGTGGTC TCACCAACAG AGCTTAACAA AGAGGTCCTG 
CCCGCCCCCC TGCCTGCCGC CACTGCGGCC CCCTCACCCA CCCTCTCGGA TGTCTTCAGC 
CTTCCGAGCC AGCCCCCGCC CGGGGACCTG TTTGGCTTGA ACCCAGTGGG ACGCAGCAGG 
TCGCCGTCCC CCTCCATCCT GCAACAGCCA ATCTCCAATA AGCCTTTTAC AACTAAGCCT 
GTCCACCTGT GGACTAAACC CGACGTGGCA GACTGGCTGG AAAGTCTAAA CCTGGGTGAA 
CATAAAGAGG CCTTCATGGA CAATGAGATC GACGGCAGCC ACCTCCCAAA CCTCCAGAAA 
GAAGACCTCA TAGATCTGGG CGTGACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT 
CTCAAACAGC TGCTGGACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015461388.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316874.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.

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CloneID	OBa89185
Clone ID Related Accession (Same CDS sequence)	XM_015461388.1 , XM_015470108.1
Accession Version	XM_015461388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGCGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTACAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316874.1
CDS	243..5777
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015461388.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461388.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGCGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTACAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHANK2 ( XM_015470108.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325594.1 Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.

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CloneID	OBa89185
Clone ID Related Accession (Same CDS sequence)	XM_015461388.1 , XM_015470108.1
Accession Version	XM_015470108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	SH3 and multiple ankyrin repeat domains protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGCGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTACAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325594.1
CDS	243..5777
Translation	MPRSPTSSEDEMAQSYSDSSEESDSDSSKEETIYDTIRATAQKPGGARAEEGQAHTLVLRIVIEDLQQTKCIRFNPDATVWVAKQRILCSLNQSLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPVGKGIPSLEFRYKKRVYRQTNLDEKQLAKLHTKTNLKKCMDHIQHRSVEKIVKMLDRGLDPNFHDLETGETPLTLAAQLDDPEEIIKALRNGGAHLDFRAKDGMTALHKAARTRNLLALKTLLELGASPDYKDSYGLTPLYHTAVVGGDPCCCELLLHEHAAVSCKDENGWHEIHQACRYGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQDSCARVLLFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDVVPFREAPAYSNRRRRPPSTLAAPRLLLRSNSDNNLHAGAPDWAVCAATSRLSLSPQLLQQTPGKADGAAKTLGSYTPGPRSRSPSLNRLGGAGEDAKRPQQPWHVGPAFPPGANKDVLSAFEYPGPRRKLYSAVPGRLFVVVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVHCKPRDSQAETRADRSKKLFRHYTVGSYDSFDASSDCVIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVTMIRQGGNHLVLKVVTVTRSLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELASVRKKKDKPEEIIPASKPSRAAESVALESRVATIRQRPTSRCFPSVADMNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTAYNCPKSPTPRVYGTIKPAFNQNPATKVSPAARSDTVATIVREKGLYHRREVDRYSLDSEDLYSRGTGPQANFRGKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSVETDPQASEQPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRARPSKFPEEGGFGEEDGAEPLSSPAPQEPENHFVGAGEAGAQGEAGRLPNAPGQAKVPEGSPAATPRSINIASPENYVHPLTGRLLDPSSPLALALSARDRALKESQQGPKGEAPKADLNKPLYIDTKMRPSGEAGFPPVGRQSGRGPLRRQETENKYETDPGKERRDDKKGVLVSIVDASQQKSAGLLMVHTVDAARPEDFLQEEDEKADPETQPDHLPSEAPEGAPETEGAFPTPAGPEPTAAAATAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRVASVPALSDLVKQKKNDTSQSPSLNSSQPTNSADSKKPAGLSNCLPASFLPPPESFDAVTDSGIEEVDSRGSSDHHLETTSTISTVSSISTLSSEGGENADTCTVYADGQAFMVDKPPVPPKPKMKPIIHKGNTLYHDALLEEDVDSFVVPPPAPPPPPGGAQPGPTKVIQPRTSRLWGDVSEVKSPILSGPKANVISELNSILQQMNREKSAKPGEGLDPTPGTKPAGLAPRGPEVISTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRNSGPRRAPSPVVSPTELNKEVLPAPLPAATAAPSPTLSDVFSLPSQPPPGDLFGLNPVGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Target ORF information:

RefSeq Version	XM_015470108.1
Organism	Bos taurus(cattle)
Definition	Bos taurus SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470108.1

ORF Insert Sequence:

ATGCCACGCA GCCCCACGTC CAGCGAGGAC GAGATGGCCC AGAGCTACTC AGACTCCTCG 
GAGGAGTCGG ACTCGGACAG TTCCAAGGAG GAGACCATCT ACGACACCAT CCGGGCCACG 
GCCCAGAAGC CGGGCGGCGC CAGGGCGGAG GAGGGCCAGG CCCACACGCT GGTGCTGCGC 
ATCGTCATCG AGGACCTGCA GCAGACGAAG TGCATTCGAT TCAACCCGGA TGCGACAGTG 
TGGGTTGCAA AGCAGCGGAT TCTGTGCTCG CTGAACCAGA GCCTGAAGGA CGTCCTGAAC 
TACGGGCTGT TCCAGCCGGC CAGCAACGGG CGCGACGGCA AGTTCCTGGA TGAGGAGCGG 
CTGCTGCGCG AGTACCCGCA GCCCGTGGGC AAGGGCATCC CGTCCCTGGA GTTTCGCTAC 
AAGAAGCGGG TGTATAGACA GACCAACCTG GACGAGAAGC AGCTGGCCAA GCTGCACACG 
AAGACCAATT TGAAAAAATG CATGGATCAC ATCCAGCATC GGTCAGTGGA GAAGATCGTC 
AAAATGCTGG ACCGAGGCCT GGATCCGAAT TTCCACGACC TGGAGACTGG AGAGACTCCC 
CTGACCTTGG CCGCTCAGCT GGACGACCCC GAGGAGATTA TCAAGGCCCT CCGGAACGGC 
GGAGCCCACC TAGACTTCCG TGCCAAGGAT GGGATGACAG CCTTGCACAA AGCCGCCCGC 
ACCAGGAACC TGCTGGCCCT GAAGACCCTC TTGGAGCTTG GCGCATCCCC GGATTATAAG 
GACAGCTACG GCCTGACCCC ACTGTATCAT ACGGCGGTGG TCGGGGGCGA CCCCTGCTGC 
TGTGAGCTGC TGCTCCACGA GCACGCGGCC GTGAGCTGCA AAGACGAGAA CGGCTGGCAC 
GAAATCCACC AGGCCTGCAG GTACGGCCAC GTGCAGCATC TGGAGCACCT GCTCTTCTAC 
GGGGCCGACA TGGGGGCCCA GAACGCCTCG GGGAACACCG CCCTGCACAT TTGCGCCCTC 
TACAACCAGG ACAGCTGTGC TCGCGTGCTT CTGTTTCGAG GTGGAGATAA GGAACTGAAG 
AACTACAACA GCCAGACCCC GTTCCAGGTG GCGATCATCG CCGGCAACTT TGAGCTAGCC 
GAGTACATCA AGAACCACAA GGAGACCGAC GTTGTGCCCT TCCGAGAGGC TCCGGCGTAC 
TCCAACCGCA GGCGGCGGCC CCCCAGCACG CTGGCCGCCC CGCGGCTCCT GCTGCGCTCC 
AACAGCGATA ACAACCTCCA CGCCGGCGCG CCCGACTGGG CTGTCTGCGC GGCCACCTCC 
CGCCTCAGCC TCTCGCCCCA GCTGCTGCAG CAGACGCCCG GCAAGGCCGA CGGGGCCGCC 
AAGACCCTCG GGAGCTACAC CCCCGGGCCC CGCAGCCGGT CGCCCTCGCT CAACAGGCTG 
GGCGGAGCCG GCGAGGATGC CAAGAGGCCC CAGCAGCCCT GGCACGTCGG CCCAGCTTTC 
CCGCCAGGAG CCAACAAGGA CGTGCTGTCT GCCTTTGAGT ACCCGGGGCC CAGACGGAAG 
CTGTACAGCG CGGTGCCTGG GAGGCTCTTC GTCGTCGTCA AGCCATACCA ACCCCAGGTC 
GATGGCGAGA TCCCCCTGCA CCGTGGCGAC AGGGTCAAAG TTCTGAGCAT CGGCGAAGGC 
GGCTTCTGGG AAGGCAGCGC CCGCGGCCAC ATCGGATGGT TCCCGGCAGA GTGTGTGGAG 
GAGGTCCACT GCAAGCCCCG GGACAGCCAG GCAGAAACTC GTGCAGACCG CAGCAAGAAA 
CTCTTCCGGC ACTACACAGT TGGCTCCTAC GACAGCTTCG ATGCCTCCAG CGACTGCGTT 
ATCGAGGAGA AGACCGTGGT CCTGCAGAAG AAAGACAATG AGGGCTTCGG ATTCGTGCTC 
CGAGGGGCGA AAGCGGATAC GCCCATCGAA GAATTCACGC CCACGCCGGC GTTCCCGGCC 
CTACAGTACC TGGAGTCTGT GGATGAAGGC GGGGTGGCAT GGCAAGCCGG ACTGAGGACC 
GGGGACTTCT TGATTGAGGT GAACAACGAG AACGTCGTCA AGGTTGGCCA CCGGCAGGTC 
GTGACCATGA TCCGCCAGGG AGGGAACCAC CTGGTCCTCA AGGTGGTCAC GGTGACCAGG 
AGTCTGGACC CGGATGACAC CGCCCGGAAG AAAGCTCCTC CGCCTCCAAA GCGAGCTCCG 
ACCACGGCCC TCACCCTGCG CTCCAAGTCC ATGACCTCGG AGCTGGAGGA GCTCGCCTCC 
GTCCGGAAGA AGAAGGATAA ACCCGAGGAG ATCATCCCAG CCTCCAAGCC ATCCCGGGCT 
GCCGAGAGCG TGGCGCTGGA GTCCAGGGTG GCCACCATCA GGCAGCGGCC CACCAGCCGG 
TGCTTCCCCT CCGTCGCGGA CATGAACTCC GTGTACGAAC GCCAGGGCAT TGCCGTGATG 
ACGCCCACTG TCCCCGGGAG CCCAAAGGGC CCGTTCCTGG GCCTCCCTCG AGGTACGATG 
CGAAGGCAGA AGTCAATAGA CAGCAGAATC TTTCTATCAG GGATAACGGA GGAGGAGCGG 
CAGTTTCTGG CCCCGCCAAT GCTCAAGTTC ACCAGAAGCC TGTCCATGCC GGACACCTCC 
GAGGACATCC CGCCCCCGCC GCAGTCAGTG CCCCCGTCTC CACCGCCACC CTCCCCCACC 
GCCTATAACT GCCCCAAGTC TCCAACTCCA CGAGTCTACG GGACAATCAA GCCCGCGTTC 
AACCAGAACC CCGCCACCAA GGTGTCCCCG GCCGCTCGCT CCGACACGGT GGCCACCATC 
GTGCGGGAGA AGGGGCTCTA CCACAGGCGG GAGGTTGACC GCTACTCCCT GGACTCCGAG 
GACCTCTACA GTCGCGGCAC CGGCCCCCAG GCCAACTTCC GCGGCAAGAG GGGCCAGATG 
CCCGAAAACC CGTACTCCGA GGTGGGGAAG ATCGCCAGCA AGGCCGTGTA CGTGCCAGCC 
AAGCCCGCCC GGCGGAAGGG CATGCTGGTG AAACAGTCCA ACGTGGAGGA CAGCCCCGAG 
AAGACGTGCT CCATCCCCAT CCCCACCATC ATCGTCAAGG AGCCCTCCAC CAGCAGCAGC 
GGCAAGAGCA GCCAGGGCAG CAGTGTGGAG ACCGACCCAC AGGCCTCGGA GCAGCCTGGC 
CAGCTGCGGC CGGACGACAG CCTGACCGTC AGCAGCCCCT TCGCCGCCGC CATCGCCGGG 
GCTGTCCGCG ACCGCGAGAA GCGGCTGGAG GCCCGGAGGA ACTCCCCAGC CTTCCTGTCC 
ACGGACCTGG GGGACGAGGA CGTGGGCCTC GGGCCGCCCG CCCCCCGGGC GCGCCCCTCC 
AAGTTCCCCG AGGAGGGTGG CTTTGGCGAG GAGGACGGCG CGGAGCCTCT GTCGTCACCG 
GCGCCCCAGG AGCCTGAGAA CCACTTTGTG GGTGCGGGCG AGGCTGGAGC TCAGGGTGAG 
GCTGGCAGGC TGCCAAACGC CCCGGGCCAA GCCAAGGTGC CGGAGGGCAG CCCCGCGGCG 
ACCCCCCGGA GCATCAACAT CGCCAGCCCC GAGAACTACG TGCACCCGCT CACGGGGCGG 
CTGCTGGACC CCAGCTCGCC GCTGGCCCTG GCGCTGTCAG CAAGGGACCG GGCCCTGAAG 
GAGTCCCAGC AGGGCCCCAA AGGGGAGGCC CCCAAGGCCG ACCTCAACAA GCCCCTCTAC 
ATCGACACCA AAATGCGGCC CAGCGGGGAA GCGGGCTTCC CTCCGGTCGG CAGGCAGAGC 
GGGCGGGGGC CCCTGCGGAG GCAGGAGACG GAGAACAAGT ACGAGACGGA CCCGGGCAAG 
GAGCGCAGGG ACGACAAGAA GGGCGTGCTC GTCAGCATCG TAGACGCCTC CCAGCAGAAA 
TCCGCCGGGC TGCTGATGGT CCACACGGTG GACGCGGCCA GGCCGGAGGA CTTCCTGCAG 
GAAGAGGACG AGAAAGCCGA CCCGGAAACG CAGCCAGACC ATCTGCCATC CGAGGCGCCC 
GAAGGCGCCC CCGAAACCGA AGGTGCTTTC CCGACCCCCG CAGGCCCCGA GCCCACGGCC 
GCCGCCGCCA CCGCGCCCGG CAGGACCATC GTGGCGGCGG GCTCCGTGGA GGAGGCGGTG 
ATCCTGCCGT TCCGCATCCC TCCCCCGCCT CTGGCATCCG TGGACCTGGA CGAGGATTTT 
ATTTTCACAG AGCCATTGCC TCCTCCCCTG GAATTTGCCA ATAGTTTTGA TATCCCCGAT 
GACCGGGTTG CTTCTGTCCC CGCTCTCTCA GACCTGGTCA AGCAGAAAAA AAACGACACC 
TCCCAGTCCC CTTCCTTGAA CTCCAGCCAG CCAACCAACT CCGCAGACAG TAAGAAGCCG 
GCTGGTCTCT CAAACTGTCT GCCCGCCTCA TTCCTGCCGC CCCCCGAAAG CTTTGACGCC 
GTCACGGACT CCGGGATCGA GGAGGTGGAC AGCCGGGGCA GCAGCGACCA CCACCTCGAG 
ACGACCAGCA CCATCTCCAC GGTGTCCAGC ATCTCCACCC TGTCCTCCGA GGGCGGGGAG 
AACGCGGACA CCTGCACAGT CTATGCAGAC GGGCAAGCCT TCATGGTTGA CAAACCCCCA 
GTACCTCCCA AGCCAAAAAT GAAGCCCATA ATCCACAAAG GCAACACCCT CTACCACGAT 
GCGCTGCTGG AGGAGGACGT GGACAGCTTC GTGGTCCCCC CGCCTGCACC TCCGCCGCCG 
CCTGGCGGGG CCCAGCCCGG CCCCACGAAG GTCATCCAGC CAAGGACCTC CAGGCTGTGG 
GGGGACGTCT CCGAGGTCAA AAGCCCGATT CTCTCAGGCC CAAAGGCGAA CGTGATTAGT 
GAGTTAAACT CAATCCTGCA GCAAATGAAC CGAGAGAAAT CTGCAAAGCC GGGGGAAGGA 
CTGGACCCAA CGCCAGGAAC CAAGCCCGCC GGCCTCGCCC CCAGAGGCCC CGAGGTCATA 
AGCACCGTCT CAGGTACACG GAGCACGACA GTCACCTTCA CCGTCCGCCC TGGGACCTCC 
CAGCCCATCA CCCTGCAGAG CCGGCCCCCG GACTACGAGA GCAGGAACTC AGGACCCAGA 
CGCGCCCCCA GCCCTGTGGT CTCACCAACA GAGCTTAACA AAGAGGTCCT GCCCGCCCCC 
CTGCCTGCCG CCACTGCGGC CCCCTCACCC ACCCTCTCGG ATGTCTTCAG CCTTCCGAGC 
CAGCCCCCGC CCGGGGACCT GTTTGGCTTG AACCCAGTGG GACGCAGCAG GTCGCCGTCC 
CCCTCCATCC TGCAACAGCC AATCTCCAAT AAGCCTTTTA CAACTAAGCC TGTCCACCTG 
TGGACTAAAC CCGACGTGGC AGACTGGCTG GAAAGTCTAA ACCTGGGTGA ACATAAAGAG 
GCCTTCATGG ACAATGAGAT CGACGGCAGC CACCTCCCAA ACCTCCAGAA AGAAGACCTC 
ATAGATCTGG GCGTGACTCG AGTCGGGCAC AGAATGAACA TAGAAAGGGC TCTCAAACAG 
CTGCTGGACA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SHANK2
Entrez Gene ID	618649
Full Name	SH3 and multiple ankyrin repeat domains 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	SHANK2	XP_005631799.1
Bos taurus (cattle)	SHANK2	XP_002699471.1
Gallus gallus (chicken)	SHANK2	XP_426415.4
Danio rerio (zebrafish)	shank2	NP_001121819.1
Rattus norvegicus (Norway rat)	Shank2	NP_958738.1
	SHANK2	XP_001099714.2
Homo sapiens (human)	SHANK2	NP_036441.2
Mus musculus (house mouse)	Shank2	NP_001106844.2
Xenopus tropicalis (tropical clawed frog)	shank2	NP_001096183.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SHANK2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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