MEGF8 cDNA ORF clone, Bos taurus(cattle)

The following MEGF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEGF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225577 XM_024979100.1
Latest version!
Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OBa225575 XM_024979098.1
Latest version!
Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa225576 XM_024979099.1
Latest version!
Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93525 XM_015467508.1
Latest version!
Bos taurus multiple EGF like domains 8 (MEGF8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93525 XM_015458238.1
Latest version!
Bos taurus multiple EGF like domains 8 (MEGF8), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225577
    Clone ID Related Accession (Same CDS sequence) XM_024979100.1
    Accession Version XM_024979100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGGCC AGCTGTGCCC CTCTCCTCCT TCCCCAGGGG GCAACGTGCA CACCCACTAC 
    CAGGAGGAAA AGTGCTATGA AGACGGCATC TTCTTCTACC ACCTGGGCTG CCATCAGTGG
    GTGTCAGGGG CTGAGCTTGC CCCTCCAGGA ACTCCAGAGG GCCGAGCAGC GCCTCCTAGT
    GGCCGGTACT CACACGTGGC GGCTGTGCTT GGTGGCAGTG TCCTGTTGGT GGCCGGGGGG
    TACAGTGGCC GGCCTCGTGG GGACTTGATG GCCTACAAAG TGCCCCCCTT TGTGTTCCAG
    GCGCCTGCTC CAGATTACCA CTTGGACTAT TGCTCCATGT ACACGGACCA CAGCGTCTGC
    TCCCGAGACC CCGAATGCAG TTGGTGTCAG GGGGCCTGCC AAGCCGCCCC ACCTCCTGGG
    ACTCCCTCAG GGGCTTGTCC GGCTGCCAGC TGCCTGGGCC TGAGCCGTCT CCTGGGTGAC
    TGCCAGGCCT GCCTGGCGTT CAGCAGCAGT GCGGCTCCCC CCCAGGGGCC CGGTGCCCTG
    GGCTGGTGCG TGCACAATGA GAGCTGCCTC CCTCGGCCTG AGCAGGCCCG CTGCCGCGGG
    GAGCAGATCT CCGGCACCGT GGGCTGGTGG GGGCCCGCTC CTGTCTTTGT GACCTCCCTG
    GAGGCCTGTG TCACCCAGAG CTTCCTGCCT GGCCTGCACC TGCTCACCTT CCAGCAGCCA
    CCCAACGCCT CCCAGCCTGA CAAGGTCTTG ATCGTCCGCA GCACGACCAT CACCCTGACG
    CCGAGCTCAG AGACCGACGT GTCCCTGGTC TACCGTGGCT TCATCCACCC GCTGCTGCCG
    GGAGGCCCTG GGGGGTCTGG GGCTGAGGAT GTGGCCGTGT GGGCCCGGGC CCAGCGCCTG
    CATGTGCTGG CCCGGATGGC CCGGGGCCCG GACACAGAAA ACATGGAGGA GGTGGGGCGC
    TGGGTGGCAC AGCAGGAGAA GGAGACGAGG CGGCTGCAGC GCCCAGGGTC AGCTCGCCTC
    TTCCCACTGC CCGGCCGGGG CCACAAGTAT GCAGTAGAGA TCCGGGGCCA GCTCAATGGC
    TCAGCAGGCC CCGGGCACAG TGAGCTCACC CTGCTGTGGG ACCGGACTGG CGTGCCAGGT
    GGCAGCGAGA TCTCCTTCTT CTTCCTGGAG CCCTACCGCT CGTCGGCCTG CGCCTCCTAC
    TCCTCCTGCC TGGGCTGCCT GGCAGACCAG GGCTGCGGCT GGTGCCTGGC CAGCGCCACC
    TGCCACCTGC GCCAGAGCGG CGCCCACTGT AGGGACGCTG GGGCCGGCGG GTCCCTGCTC
    GTGCTGGTGC CTGCCCTCTG CCCGCTCTGC GAGGAGCATC GCGACTGCCA CGCCTGCACC
    CAGGACCCCT TCTGTGAGTG GCATCAGAGC ACCAGCCGCA AGGGGGACGC GGCGTGCAGC
    CGGCGCGGCC GGGGTCGGGG CGCCCTGAAG AGTCCGGAAG AGTGCCCCCC ACTCTGCAGC
    CAGCGCCTGA CCTGTGACGA CTGCCTGGCC AACTCGAGCC AGTGCGCCTG GTGCCAGTCC
    ACCCGCACCT GCTTCCTGTT CGCCGCCTAC CTGGCCCGGT ACCCGCACGG GGGCTGCCGA
    GGCTGGGACG ACAGCGTGCA CTCGGAGCCT CGGTGCCGGA GCTGTGACCG CTTCCCGAGC
    TGTCACGAGT GTCTGCAGAG CCATGAGTGT GGCTGGTGTG GAAACGAGGA CAACCCCACG
    CTGGGCCGTT GCCTCCAAGG GGACTTCTCA GGGCCTCTGA GTGGGGGGAA CTGCTCCCTG
    TGGGTCGGGG AGGGCCTGGG GCTGTCTGTG GCCCTCCCCG CCCGCTGGGC GTATGCCCGC
    TGTCCGGATG TAGACGAGTG CCGCCTGGGC CTGGCGCGGT GCCACCTGCG GGCGACCTGC
    CTGAACACAC CCCTCAGCTA CGAGTGTCAC TGCCAGAGGG GCTACCAGGG CGATGGCGTC
    ACTTACTGCA ACCGCACGTG CCTGGAGGAC TGTGGCCACG GTGTGTGCAG CGGCCCCCCA
    GACTTCACCT GCGTGTGTGA CCTGGGCTGG ACATCAGACC TGCCCCTTCC CACGCCGGCC
    CCCGGCCCCC CCGCCCCCCG CTGTTCGCGG GACTGTGGCT GCAACTTCCA CAGCCACTGC
    CACAAGCGGG GGCCCGGCTT CTGCGATGAG TGCCAGGACT GGACGTGGGG GGAGCACTGT
    GAGCGGTGCC GGCCTGGAAG CTTCGGGAAT GCCACAGGCT CCGGCGGCTG CCGGCCCTGC
    CAGTGCAACG GGCATGGGGA CCCTCGCCGA GGCCACTGTG ACAACCTCAG CGGGCTCTGC
    TTCTGCCAGG ACCACACCGA GGGTGCCCAC TGCCAGCTCT GCTCCCCTGG CTACTACGGG
    GACCCTCGGG CAGGTGGCTC CTGCTTCCGG GAGTGTGGGG GTCGTGCCCT CCTCACCAAC
    GTGTCCTCAG TGGCACTGGG CTCACGCCGG TTTGGGGGGC TCCTGCCTGC AGGTGGCGGG
    GCAGCAAGAG CGGGGCCTGG CCTGTCCTAC TGTGTGTGGG TTGTCTCGGC CACTGAGGTG
    CTGCAGCCCT GTGCCCCCGG GACCCTCTGT CCCCCGCTTA CCCTCACCTT CTCCCCCGAC
    AGCAGCACCC CGTGCACGCT GAGCTATGTC CTGGCCTTTG ATGGATTCCC ACGCTTCCTA
    GACACTGGTG TCGTCCAGTC GGACCGTAGC CTCATCGCTG CGTTTTGCGG CCAGCGGCGG
    GACCGGCCCC TCACTGTGCA GGCCCTGTCT GGGCTGCTGG TACTGCACTG GGAGGCCAAT
    GGCTCCTCAT CCTGGGGCTT CAATGCCTCC GTGGGCTCTG CCCGCTGTGG GTCAGGGGGC
    CCCGGGAGCT GCCCTGTCCC CCAGGAGTGT GTGCCCCAGG ATGGGGTCCC AGGTGCTGGA
    CTCTGCCGGT GTCCCCAGGG CTGGGCTGGC CCGCACTGCC GCATGGCTGT GTGTCCTGAG
    AACTGTAATG CCCACAACGG GGCGGGGACG TGTAATCAGA GCCTGGGCGT GTGCGTCTGT
    GCTGAGGGCT TTGGGGGTCC TGACTGTGCC ACGAAGCTGG ATGGTGGGCA GCTGGTCTGG
    GAGACCCTCA TGGACAGCCG CCTCTCAGCT GACACTGCCA GCCGCTTCCT GCACCGCCTG
    GGGCACACGA TGGTGGAGGG ACCTGATGCC ACCTTGTGGA TGTTTGGGGG CCTGGGCCTG
    CCCCAGGGGC TGCTGGGAAA CCTGTATAGG TACTCAGTGA GTGAACGGCG GTGGACACAG
    ATGTTGGCAG GAGCCGAGGA TGGGGGCCCA GGCCCCTCCC CTCGCTCTTT CCACGCTGCT
    GCCTACGTGC CTGCTGGCCG TGGGGCCATG TACCTGCTGG GGGGGCTCAC AGCTGGGGGC
    ATCGCCCGGG ACTTCTGGGT CCTCAACCTC ACCACCCTGC AGTGGCGGCA GGAGAAGGCC
    CCTCAGACTG TGGAGCTGCC AGCCGTTGCT GGTCACACGC TTACTGCCCG CCGAGGCCTA
    TCTCTGCTCC TGGTGGGTGG TTACTCCCCG GAAAATGGCT TCAACCAGCA GCTGCTTGAG
    TACCAGCTGG CGACTGGCAC CTGGGTGTCC GGGGCTCAGA GCGGGACACC CCCCACAGGT
    CTCTACGGTC ACTCTGCTGT GTATCATGAG GCCACCGACT CCCTCTACGT GTTCGGGGGT
    TTCCGCTTCC ACGTGGAGCT GGCAGCCCCG TCCCCTGAGC TCTACTCCCT GCACTGCCCT
    GACCGCACCT GGAGCCTGCT GGCCCCTTCT CAGGGAGCAA AGCCCCGGCC CCGACTCTTC
    CACGCCTCGG CCTTGCTAGG GGACACCATG GTGGTCCTCG GGGGGCGCTC AGACCCCGAC
    GAGTTCAGCA GCGACGTGCT GCTCTACCAG GTCAACTGCA ACACCTGGCT CCTGCCCGAC
    CTCATCGGCC CAGCCTCTGT GGGACCGCCA ATGGAAGAGT CTGTGGCTCA CGCCGTGGCC
    GCGGTAGGGG GTCACCTGTT TATCTCAGGA GGCTTTGGGG GAGTGGCCCT GGGCCGCCTG
    CTGGCCCTGA CCCTGCCCCC TGACCCCTGC CGCCTGCTGC CCTCGCCCGA AGCTTGTAAC
    CAGACTGGGG CCTGTACCTG GTGCCATGGA GCCTGCCTGT CTGGGGACCA GGCCCACAGG
    CTGGGCTGTG GCGGCACCCC CTGCTCCCCT ATGCCCCACT CCCCCGAGGA ATGTCGACGT
    CTGCGGACCT GCAGCGAGTG CCTGGCCCGC CATCCCCGGA CCCTGCAGCC TGGAGATGGA
    GAGGCCTCTG CCCCACGCTG TAAGTGGTGT ACCAACTGCC CCGAGGGTGC CTGCATTGGG
    CGCAACGGGT CCTGCACCTC AGAGAATGAC TGTCGCATCA ACCAGCGCGA GGTCTTCTGG
    GCCGGGAACT GCTCGGAGGC TGTGTGCGGG GCGGCCGACT GCGAGCAGTG CACCAGGGAG
    GGCAAATGCA TGTGGACGCG GCAGTTCAAG AGGACGGGGG AGACCCGCCG CATCCTTTCG
    GTGCAGCCCA CCTACGACTG GACGTGCTTC AGCCACTCAC TGCTGAACGT GTCCCCGATG
    CCAGTGGAGT CATCGCCCCC GCTGCCCTGC CCCACACCCT GTCACCTCCT GCCCAACTGC
    ACCTCCTGCC TGGACTCCAA GGGTGCTGAC GGGGGCTGGC AGCACTGCGT CTGGAGCAGC
    AGCCTCCAGC AGTGTCTGAG CCCCTCCTAT CTGCCCCTGC GGTGTATGGC CGGAGGCTGT
    GGGCGGCTGC TCCGCGGACC CGAGAGCTGC TCCCTGGGCT GTGCTCAGGC AACCCAGTGT
    GCCCTGTGCC TGCGGCGCCC CCACTGTGGC TGGTGTACCT GGGGGGGCCA GGATGGGGGC
    GGCCGCTGCC TGGAGGGTGG ACTCAGTGGC CCCCGTGACG GGCTGACGTG TGGGCGACCT
    GGGGCCTCCT GGGCCTTTCT TTCCTGCCCC CCGGAGGACG AATGTGCCAA CGGGCACCAT
    GACTGCAACG AGACCCAGAA CTGCCACGAC CGGCCCCACG GTTACGAGTG CAGCTGCAAG
    GCAGGCTACA CCATGGACAA CGTGACGGGG CTGTGCCGCC CAGTGTGCGC CCAGGGTTGC
    GTGAACGGCT CGTGTGTGGA GCCCGACCAC TGCCGCTGCC ACTTCGGCTT CGTCGGCCGC
    AACTGTTCCA CGGAGTGTCG CTGCAACCGC CACAGCGACT GCGCCGGCGT GGGGGCCCGC
    GACCACTGCC TGCTCTGCCA CAATCACACC AAGGGCAGCC ACTGTGAGCA GTGCCTCCCG
    CTGTTCGTGG GCTCAGCCGT AGGGGGAGGG ACCTGCCGGC CCTGCCACGC CTTTTGTCGC
    GGAAACAGCC ACGTCTGCGT CTCCAGGAAG GAGCTTGAGA TAGCCAGGAG GGAGCCGGAG
    AAGTACTCGC TGGATCCAGA TGAGATTGAA CACTGGGCGA CAGAGGGTCC CAGCGAAGAT
    GAGGCTGTGT GTGTGAACTG CCAGAATAAC AGCTATGGGG ATAAGTGTGA GAGCTGCCTC
    CACGGCTACT TCCTCCTCGA CGGGAAATGC ACCAAATGCC AGTGTAATGG CCACGCGGAC
    ACATGTAATG AGCAGGATGG GACAGGCTGC CCATGTCAGA ACAACACAGA GACAGGCACG
    TGCCAGGGCA GCTCCCCCAG TGACCGCCGA GACTGCTACA AATACCAGTG TGCCAAGTGC
    CGAGAGTCAT TCCACGGGAG CCCGCTAGGG GGCCAGCAGT GCTACCGCCT CATCTCCGTG
    GAACAGGAGT ACTGCCTGGA CCCCACGTCC CAAACCAACT GTTTCCACGA GCCCAAGCGC
    CGGGCTCTGG GTCCCGGACG CACCGTTCTC TTTGGAGTGC AGCCTAAGTT CACCAACGTG
    GACATCCGCC TGACGCTGGA CGTGACCTTT GGCGCCGTGG ACCTCTACGT CTCCACCTCC
    TATGACACCT TCGTGGTCCG CGTGGCTCCC GACACTGGTG TCCACACCGT GCATATCCAG
    CCGCCACCAC CTCCCCCACC CCCACCACCC CCTGCTGACG GCGGGCCCCG GGGACCTGGG
    GATCTCGGGG GACCTGGGGC TGGAGGTGGG CCGGCTACCC CAGCAGAGCC GCGAGTGCGG
    GAGGTCTGGC CACGGGGCCT GATCACCTAC GTGACAGTGA CAGAGCCGTC GGCGGTGCTG
    GTGGTCCGTG GCGTGAGGGA CCGGCTGGTC ATCACCTACC CGCACGAGCA CCACACCCTC
    AAGTCCAGCC GCTTCTACCT GCTCCTGCTG GGTGTGGGAG ACCCAAGTGG GCCCAGTGCC
    AACGGCTCAG CTGACTCGCA GGGCTTGCTG TTCTTCCGGC AGGACCAGGC CCACATTGAC
    CTGTTTGTCT TCTTCTCTGT CTTCTTCTCC TGCTTCTTCC TCTTCCTCTC GCTCTGTGTG
    CTGCTCTGGA AGGCCAAGCA GGCCCTGGAC CACCGGCAGG AACAGCGCCG GCACCTGCAG
    GAGATGACCA AGATGGCCAG CCGCCCCTTC GCCAAGGTCA CTGTCTGCTT CCCGCCAGAC
    CCTGCTGCCC CAGCCCCCAC CTGGAAGCCT GCCGGGCTCC CGCCACCCGC CTTCCGCCGC
    TCCGAGCCCT TCTTGGCACC CTTGCTGCTG ACCGGGGCCG GCGGGCCCTG GGGACCTGTA
    GGAGGGGGCT GCTGCCCACC GGCCATCCCC ACCACCACTG CCGGCCTGCG AGCCGGGCCT
    ATCACCCTTG AGCCCACCGA GGATGGCATG GCCGGCGTGG CCACACTGCT GCTCCAACTG
    CCTGGCGGGC CCCACGCACC CAACGGCGCC TGCCTGGGGT CAGCGCTCGT CACACTGCGG
    CACCGGCTGC ACGAATACTG CGGCGGCGGC GGGGGCGCTG GGGGCAGTGG GCATGGGGCT
    GGTGCGGGCC GGAAGGGGCT GTTGAGCCAG GACAACCTCA CCAGCATGTC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834868.1
    CDS127..7143
    Translation

    Target ORF information:

    RefSeq Version XM_024979100.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTGGCC AGCTGTGCCC CTCTCCTCCT TCCCCAGGGG GCAACGTGCA CACCCACTAC 
    CAGGAGGAAA AGTGCTATGA AGACGGCATC TTCTTCTACC ACCTGGGCTG CCATCAGTGG
    GTGTCAGGGG CTGAGCTTGC CCCTCCAGGA ACTCCAGAGG GCCGAGCAGC GCCTCCTAGT
    GGCCGGTACT CACACGTGGC GGCTGTGCTT GGTGGCAGTG TCCTGTTGGT GGCCGGGGGG
    TACAGTGGCC GGCCTCGTGG GGACTTGATG GCCTACAAAG TGCCCCCCTT TGTGTTCCAG
    GCGCCTGCTC CAGATTACCA CTTGGACTAT TGCTCCATGT ACACGGACCA CAGCGTCTGC
    TCCCGAGACC CCGAATGCAG TTGGTGTCAG GGGGCCTGCC AAGCCGCCCC ACCTCCTGGG
    ACTCCCTCAG GGGCTTGTCC GGCTGCCAGC TGCCTGGGCC TGAGCCGTCT CCTGGGTGAC
    TGCCAGGCCT GCCTGGCGTT CAGCAGCAGT GCGGCTCCCC CCCAGGGGCC CGGTGCCCTG
    GGCTGGTGCG TGCACAATGA GAGCTGCCTC CCTCGGCCTG AGCAGGCCCG CTGCCGCGGG
    GAGCAGATCT CCGGCACCGT GGGCTGGTGG GGGCCCGCTC CTGTCTTTGT GACCTCCCTG
    GAGGCCTGTG TCACCCAGAG CTTCCTGCCT GGCCTGCACC TGCTCACCTT CCAGCAGCCA
    CCCAACGCCT CCCAGCCTGA CAAGGTCTTG ATCGTCCGCA GCACGACCAT CACCCTGACG
    CCGAGCTCAG AGACCGACGT GTCCCTGGTC TACCGTGGCT TCATCCACCC GCTGCTGCCG
    GGAGGCCCTG GGGGGTCTGG GGCTGAGGAT GTGGCCGTGT GGGCCCGGGC CCAGCGCCTG
    CATGTGCTGG CCCGGATGGC CCGGGGCCCG GACACAGAAA ACATGGAGGA GGTGGGGCGC
    TGGGTGGCAC AGCAGGAGAA GGAGACGAGG CGGCTGCAGC GCCCAGGGTC AGCTCGCCTC
    TTCCCACTGC CCGGCCGGGG CCACAAGTAT GCAGTAGAGA TCCGGGGCCA GCTCAATGGC
    TCAGCAGGCC CCGGGCACAG TGAGCTCACC CTGCTGTGGG ACCGGACTGG CGTGCCAGGT
    GGCAGCGAGA TCTCCTTCTT CTTCCTGGAG CCCTACCGCT CGTCGGCCTG CGCCTCCTAC
    TCCTCCTGCC TGGGCTGCCT GGCAGACCAG GGCTGCGGCT GGTGCCTGGC CAGCGCCACC
    TGCCACCTGC GCCAGAGCGG CGCCCACTGT AGGGACGCTG GGGCCGGCGG GTCCCTGCTC
    GTGCTGGTGC CTGCCCTCTG CCCGCTCTGC GAGGAGCATC GCGACTGCCA CGCCTGCACC
    CAGGACCCCT TCTGTGAGTG GCATCAGAGC ACCAGCCGCA AGGGGGACGC GGCGTGCAGC
    CGGCGCGGCC GGGGTCGGGG CGCCCTGAAG AGTCCGGAAG AGTGCCCCCC ACTCTGCAGC
    CAGCGCCTGA CCTGTGACGA CTGCCTGGCC AACTCGAGCC AGTGCGCCTG GTGCCAGTCC
    ACCCGCACCT GCTTCCTGTT CGCCGCCTAC CTGGCCCGGT ACCCGCACGG GGGCTGCCGA
    GGCTGGGACG ACAGCGTGCA CTCGGAGCCT CGGTGCCGGA GCTGTGACCG CTTCCCGAGC
    TGTCACGAGT GTCTGCAGAG CCATGAGTGT GGCTGGTGTG GAAACGAGGA CAACCCCACG
    CTGGGCCGTT GCCTCCAAGG GGACTTCTCA GGGCCTCTGA GTGGGGGGAA CTGCTCCCTG
    TGGGTCGGGG AGGGCCTGGG GCTGTCTGTG GCCCTCCCCG CCCGCTGGGC GTATGCCCGC
    TGTCCGGATG TAGACGAGTG CCGCCTGGGC CTGGCGCGGT GCCACCTGCG GGCGACCTGC
    CTGAACACAC CCCTCAGCTA CGAGTGTCAC TGCCAGAGGG GCTACCAGGG CGATGGCGTC
    ACTTACTGCA ACCGCACGTG CCTGGAGGAC TGTGGCCACG GTGTGTGCAG CGGCCCCCCA
    GACTTCACCT GCGTGTGTGA CCTGGGCTGG ACATCAGACC TGCCCCTTCC CACGCCGGCC
    CCCGGCCCCC CCGCCCCCCG CTGTTCGCGG GACTGTGGCT GCAACTTCCA CAGCCACTGC
    CACAAGCGGG GGCCCGGCTT CTGCGATGAG TGCCAGGACT GGACGTGGGG GGAGCACTGT
    GAGCGGTGCC GGCCTGGAAG CTTCGGGAAT GCCACAGGCT CCGGCGGCTG CCGGCCCTGC
    CAGTGCAACG GGCATGGGGA CCCTCGCCGA GGCCACTGTG ACAACCTCAG CGGGCTCTGC
    TTCTGCCAGG ACCACACCGA GGGTGCCCAC TGCCAGCTCT GCTCCCCTGG CTACTACGGG
    GACCCTCGGG CAGGTGGCTC CTGCTTCCGG GAGTGTGGGG GTCGTGCCCT CCTCACCAAC
    GTGTCCTCAG TGGCACTGGG CTCACGCCGG TTTGGGGGGC TCCTGCCTGC AGGTGGCGGG
    GCAGCAAGAG CGGGGCCTGG CCTGTCCTAC TGTGTGTGGG TTGTCTCGGC CACTGAGGTG
    CTGCAGCCCT GTGCCCCCGG GACCCTCTGT CCCCCGCTTA CCCTCACCTT CTCCCCCGAC
    AGCAGCACCC CGTGCACGCT GAGCTATGTC CTGGCCTTTG ATGGATTCCC ACGCTTCCTA
    GACACTGGTG TCGTCCAGTC GGACCGTAGC CTCATCGCTG CGTTTTGCGG CCAGCGGCGG
    GACCGGCCCC TCACTGTGCA GGCCCTGTCT GGGCTGCTGG TACTGCACTG GGAGGCCAAT
    GGCTCCTCAT CCTGGGGCTT CAATGCCTCC GTGGGCTCTG CCCGCTGTGG GTCAGGGGGC
    CCCGGGAGCT GCCCTGTCCC CCAGGAGTGT GTGCCCCAGG ATGGGGTCCC AGGTGCTGGA
    CTCTGCCGGT GTCCCCAGGG CTGGGCTGGC CCGCACTGCC GCATGGCTGT GTGTCCTGAG
    AACTGTAATG CCCACAACGG GGCGGGGACG TGTAATCAGA GCCTGGGCGT GTGCGTCTGT
    GCTGAGGGCT TTGGGGGTCC TGACTGTGCC ACGAAGCTGG ATGGTGGGCA GCTGGTCTGG
    GAGACCCTCA TGGACAGCCG CCTCTCAGCT GACACTGCCA GCCGCTTCCT GCACCGCCTG
    GGGCACACGA TGGTGGAGGG ACCTGATGCC ACCTTGTGGA TGTTTGGGGG CCTGGGCCTG
    CCCCAGGGGC TGCTGGGAAA CCTGTATAGG TACTCAGTGA GTGAACGGCG GTGGACACAG
    ATGTTGGCAG GAGCCGAGGA TGGGGGCCCA GGCCCCTCCC CTCGCTCTTT CCACGCTGCT
    GCCTACGTGC CTGCTGGCCG TGGGGCCATG TACCTGCTGG GGGGGCTCAC AGCTGGGGGC
    ATCGCCCGGG ACTTCTGGGT CCTCAACCTC ACCACCCTGC AGTGGCGGCA GGAGAAGGCC
    CCTCAGACTG TGGAGCTGCC AGCCGTTGCT GGTCACACGC TTACTGCCCG CCGAGGCCTA
    TCTCTGCTCC TGGTGGGTGG TTACTCCCCG GAAAATGGCT TCAACCAGCA GCTGCTTGAG
    TACCAGCTGG CGACTGGCAC CTGGGTGTCC GGGGCTCAGA GCGGGACACC CCCCACAGGT
    CTCTACGGTC ACTCTGCTGT GTATCATGAG GCCACCGACT CCCTCTACGT GTTCGGGGGT
    TTCCGCTTCC ACGTGGAGCT GGCAGCCCCG TCCCCTGAGC TCTACTCCCT GCACTGCCCT
    GACCGCACCT GGAGCCTGCT GGCCCCTTCT CAGGGAGCAA AGCCCCGGCC CCGACTCTTC
    CACGCCTCGG CCTTGCTAGG GGACACCATG GTGGTCCTCG GGGGGCGCTC AGACCCCGAC
    GAGTTCAGCA GCGACGTGCT GCTCTACCAG GTCAACTGCA ACACCTGGCT CCTGCCCGAC
    CTCATCGGCC CAGCCTCTGT GGGACCGCCA ATGGAAGAGT CTGTGGCTCA CGCCGTGGCC
    GCGGTAGGGG GTCACCTGTT TATCTCAGGA GGCTTTGGGG GAGTGGCCCT GGGCCGCCTG
    CTGGCCCTGA CCCTGCCCCC TGACCCCTGC CGCCTGCTGC CCTCGCCCGA AGCTTGTAAC
    CAGACTGGGG CCTGTACCTG GTGCCATGGA GCCTGCCTGT CTGGGGACCA GGCCCACAGG
    CTGGGCTGTG GCGGCACCCC CTGCTCCCCT ATGCCCCACT CCCCCGAGGA ATGTCGACGT
    CTGCGGACCT GCAGCGAGTG CCTGGCCCGC CATCCCCGGA CCCTGCAGCC TGGAGATGGA
    GAGGCCTCTG CCCCACGCTG TAAGTGGTGT ACCAACTGCC CCGAGGGTGC CTGCATTGGG
    CGCAACGGGT CCTGCACCTC AGAGAATGAC TGTCGCATCA ACCAGCGCGA GGTCTTCTGG
    GCCGGGAACT GCTCGGAGGC TGTGTGCGGG GCGGCCGACT GCGAGCAGTG CACCAGGGAG
    GGCAAATGCA TGTGGACGCG GCAGTTCAAG AGGACGGGGG AGACCCGCCG CATCCTTTCG
    GTGCAGCCCA CCTACGACTG GACGTGCTTC AGCCACTCAC TGCTGAACGT GTCCCCGATG
    CCAGTGGAGT CATCGCCCCC GCTGCCCTGC CCCACACCCT GTCACCTCCT GCCCAACTGC
    ACCTCCTGCC TGGACTCCAA GGGTGCTGAC GGGGGCTGGC AGCACTGCGT CTGGAGCAGC
    AGCCTCCAGC AGTGTCTGAG CCCCTCCTAT CTGCCCCTGC GGTGTATGGC CGGAGGCTGT
    GGGCGGCTGC TCCGCGGACC CGAGAGCTGC TCCCTGGGCT GTGCTCAGGC AACCCAGTGT
    GCCCTGTGCC TGCGGCGCCC CCACTGTGGC TGGTGTACCT GGGGGGGCCA GGATGGGGGC
    GGCCGCTGCC TGGAGGGTGG ACTCAGTGGC CCCCGTGACG GGCTGACGTG TGGGCGACCT
    GGGGCCTCCT GGGCCTTTCT TTCCTGCCCC CCGGAGGACG AATGTGCCAA CGGGCACCAT
    GACTGCAACG AGACCCAGAA CTGCCACGAC CGGCCCCACG GTTACGAGTG CAGCTGCAAG
    GCAGGCTACA CCATGGACAA CGTGACGGGG CTGTGCCGCC CAGTGTGCGC CCAGGGTTGC
    GTGAACGGCT CGTGTGTGGA GCCCGACCAC TGCCGCTGCC ACTTCGGCTT CGTCGGCCGC
    AACTGTTCCA CGGAGTGTCG CTGCAACCGC CACAGCGACT GCGCCGGCGT GGGGGCCCGC
    GACCACTGCC TGCTCTGCCA CAATCACACC AAGGGCAGCC ACTGTGAGCA GTGCCTCCCG
    CTGTTCGTGG GCTCAGCCGT AGGGGGAGGG ACCTGCCGGC CCTGCCACGC CTTTTGTCGC
    GGAAACAGCC ACGTCTGCGT CTCCAGGAAG GAGCTTGAGA TAGCCAGGAG GGAGCCGGAG
    AAGTACTCGC TGGATCCAGA TGAGATTGAA CACTGGGCGA CAGAGGGTCC CAGCGAAGAT
    GAGGCTGTGT GTGTGAACTG CCAGAATAAC AGCTATGGGG ATAAGTGTGA GAGCTGCCTC
    CACGGCTACT TCCTCCTCGA CGGGAAATGC ACCAAATGCC AGTGTAATGG CCACGCGGAC
    ACATGTAATG AGCAGGATGG GACAGGCTGC CCATGTCAGA ACAACACAGA GACAGGCACG
    TGCCAGGGCA GCTCCCCCAG TGACCGCCGA GACTGCTACA AATACCAGTG TGCCAAGTGC
    CGAGAGTCAT TCCACGGGAG CCCGCTAGGG GGCCAGCAGT GCTACCGCCT CATCTCCGTG
    GAACAGGAGT ACTGCCTGGA CCCCACGTCC CAAACCAACT GTTTCCACGA GCCCAAGCGC
    CGGGCTCTGG GTCCCGGACG CACCGTTCTC TTTGGAGTGC AGCCTAAGTT CACCAACGTG
    GACATCCGCC TGACGCTGGA CGTGACCTTT GGCGCCGTGG ACCTCTACGT CTCCACCTCC
    TATGACACCT TCGTGGTCCG CGTGGCTCCC GACACTGGTG TCCACACCGT GCATATCCAG
    CCGCCACCAC CTCCCCCACC CCCACCACCC CCTGCTGACG GCGGGCCCCG GGGACCTGGG
    GATCTCGGGG GACCTGGGGC TGGAGGTGGG CCGGCTACCC CAGCAGAGCC GCGAGTGCGG
    GAGGTCTGGC CACGGGGCCT GATCACCTAC GTGACAGTGA CAGAGCCGTC GGCGGTGCTG
    GTGGTCCGTG GCGTGAGGGA CCGGCTGGTC ATCACCTACC CGCACGAGCA CCACACCCTC
    AAGTCCAGCC GCTTCTACCT GCTCCTGCTG GGTGTGGGAG ACCCAAGTGG GCCCAGTGCC
    AACGGCTCAG CTGACTCGCA GGGCTTGCTG TTCTTCCGGC AGGACCAGGC CCACATTGAC
    CTGTTTGTCT TCTTCTCTGT CTTCTTCTCC TGCTTCTTCC TCTTCCTCTC GCTCTGTGTG
    CTGCTCTGGA AGGCCAAGCA GGCCCTGGAC CACCGGCAGG AACAGCGCCG GCACCTGCAG
    GAGATGACCA AGATGGCCAG CCGCCCCTTC GCCAAGGTCA CTGTCTGCTT CCCGCCAGAC
    CCTGCTGCCC CAGCCCCCAC CTGGAAGCCT GCCGGGCTCC CGCCACCCGC CTTCCGCCGC
    TCCGAGCCCT TCTTGGCACC CTTGCTGCTG ACCGGGGCCG GCGGGCCCTG GGGACCTGTA
    GGAGGGGGCT GCTGCCCACC GGCCATCCCC ACCACCACTG CCGGCCTGCG AGCCGGGCCT
    ATCACCCTTG AGCCCACCGA GGATGGCATG GCCGGCGTGG CCACACTGCT GCTCCAACTG
    CCTGGCGGGC CCCACGCACC CAACGGCGCC TGCCTGGGGT CAGCGCTCGT CACACTGCGG
    CACCGGCTGC ACGAATACTG CGGCGGCGGC GGGGGCGCTG GGGGCAGTGG GCATGGGGCT
    GGTGCGGGCC GGAAGGGGCT GTTGAGCCAG GACAACCTCA CCAGCATGTC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225575
    Clone ID Related Accession (Same CDS sequence) XM_024979098.1
    Accession Version XM_024979098.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGAGCAGGC CCGCTGCCGC GGGGAGCAGA TCTCCGGCAC CGTGGGCTGG
    TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG
    CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC
    TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA CGTGTCCCTG
    GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG
    GATGTGGCCG TGTGGGCCCG GGCCCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC
    CCGGACACAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG
    AGGCGGCTGC AGCGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG
    TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGTGAGCTC
    ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG
    GAGCCCTACC GCTCGTCGGC CTGCGCCTCC TACTCCTCCT GCCTGGGCTG CCTGGCAGAC
    CAGGGCTGCG GCTGGTGCCT GGCCAGCGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC
    TGTAGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC
    TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG
    AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG
    AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGCC TGACCTGTGA CGACTGCCTG
    GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC
    TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG
    CCTCGGTGCC GGAGCTGTGA CCGCTTCCCG AGCTGTCACG AGTGTCTGCA GAGCCATGAG
    TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834866.1
    CDS767..9136
    Translation

    Target ORF information:

    RefSeq Version XM_024979098.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979098.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGAGCAGGC CCGCTGCCGC GGGGAGCAGA TCTCCGGCAC CGTGGGCTGG
    TGGGGGCCCG CTCCTGTCTT TGTGACCTCC CTGGAGGCCT GTGTCACCCA GAGCTTCCTG
    CCTGGCCTGC ACCTGCTCAC CTTCCAGCAG CCACCCAACG CCTCCCAGCC TGACAAGGTC
    TTGATCGTCC GCAGCACGAC CATCACCCTG ACGCCGAGCT CAGAGACCGA CGTGTCCCTG
    GTCTACCGTG GCTTCATCCA CCCGCTGCTG CCGGGAGGCC CTGGGGGGTC TGGGGCTGAG
    GATGTGGCCG TGTGGGCCCG GGCCCAGCGC CTGCATGTGC TGGCCCGGAT GGCCCGGGGC
    CCGGACACAG AAAACATGGA GGAGGTGGGG CGCTGGGTGG CACAGCAGGA GAAGGAGACG
    AGGCGGCTGC AGCGCCCAGG GTCAGCTCGC CTCTTCCCAC TGCCCGGCCG GGGCCACAAG
    TATGCAGTAG AGATCCGGGG CCAGCTCAAT GGCTCAGCAG GCCCCGGGCA CAGTGAGCTC
    ACCCTGCTGT GGGACCGGAC TGGCGTGCCA GGTGGCAGCG AGATCTCCTT CTTCTTCCTG
    GAGCCCTACC GCTCGTCGGC CTGCGCCTCC TACTCCTCCT GCCTGGGCTG CCTGGCAGAC
    CAGGGCTGCG GCTGGTGCCT GGCCAGCGCC ACCTGCCACC TGCGCCAGAG CGGCGCCCAC
    TGTAGGGACG CTGGGGCCGG CGGGTCCCTG CTCGTGCTGG TGCCTGCCCT CTGCCCGCTC
    TGCGAGGAGC ATCGCGACTG CCACGCCTGC ACCCAGGACC CCTTCTGTGA GTGGCATCAG
    AGCACCAGCC GCAAGGGGGA CGCGGCGTGC AGCCGGCGCG GCCGGGGTCG GGGCGCCCTG
    AAGAGTCCGG AAGAGTGCCC CCCACTCTGC AGCCAGCGCC TGACCTGTGA CGACTGCCTG
    GCCAACTCGA GCCAGTGCGC CTGGTGCCAG TCCACCCGCA CCTGCTTCCT GTTCGCCGCC
    TACCTGGCCC GGTACCCGCA CGGGGGCTGC CGAGGCTGGG ACGACAGCGT GCACTCGGAG
    CCTCGGTGCC GGAGCTGTGA CCGCTTCCCG AGCTGTCACG AGTGTCTGCA GAGCCATGAG
    TGTGGCTGGT GTGGAAACGA GGACAACCCC ACGCTGGGCC GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACGCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225576
    Clone ID Related Accession (Same CDS sequence) XM_024979099.1
    Accession Version XM_024979099.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGCTGCTGC ACCTCTTCAG CGATGCCAAC TACAACCTGC TGGGCTTCAA CGCCTCCTTC 
    CGCTTCTCCC TGTGCCCGGG TGGCTGCCGC AGCCATGGGC AGTGCCAGCC CCCAGGGGTG
    TGTGTCTGTG AGCCGGGCTG GGGGGGCCCC GACTGCAGCC TGCAGGAGTG CTCAGCCTAC
    TGCGGCAGCC ATGGCACCTG CACCTCGCCC CTGGGACCGT GCCGCTGTGA GCCTGGTTTC
    CTGGGACGCG CCTGTGACCT GCACCTGTGG GAGAACCAGG GGGCTGGCTG GTGGCACAAC
    GTGAGTGCCG GGGACCCCGC CTTCTCTGCC CGTATCGGGG CAGCCGGGGC CTTCCTGTCC
    CCACTGGGGC TGCTGGCTGT TTTTGGAGGC CAGGACCTCA ACCGCGCGCT GGGGGACCTC
    GTCCTGTACA ACTTCTCTGC CAACACCTGG GAGCGCTGGG ACCTGAGCCC TGCCCCGGCT
    GCCCGTCACT CCCACGTGGC TGTGGCTTGG GCCGGCTCCC TGGTGCTGAT GGGCGGTGAG
    CTGGCTGATG GCACCCTCAC CAGCGACATC TGGGCCTTTA GCCCGATGGG TGGCGGCCGC
    TGGGAGCTTC TGGCACCACC TGCCTCCAGT CCCTCGGGGC CGCCCGGCCT GGCAGGTCAT
    GCAGCAGCCC TGGTGGACAA CACGTGGCTC TATGTGTCTG GTGGCCGCAC CCAGCACGAC
    CTCTTCTCCT CCGGCCTCTT CCGTTTCCGC CTCGACGGCA CCAGTGGGGG CTATTGGGAG
    CAGGTGATGC CAGCAGGTGG GCGGCCCCCT GCCGCCACCG GCCATTCCAT GGTGTTCCAT
    GCCGCCTCCC GTGCCCTGCT GGTTCACGGT GGACACCGGC CCTCCACTGC CCGATTCTCT
    GTGCGGGTGA ACTCCACCGA GCTCTTCCAT GTGGATCGGC GCGTGTGGAC CACCCTGAAG
    GGCCGGGAGG GACTTCACGG CCCACGGGAG CGAGCCTTCC ACACGGCCAG CGTCCTGGGC
    AACTACATGC TGGTCTATGG GGGCAACGTG CACACCCACT ACCAGGAGGA AAAGTGCTAT
    GAAGACGGCA TCTTCTTCTA CCACCTGGGC TGCCATCAGT GGGTGTCAGG GGCTGAGCTT
    GCCCCTCCAG GAACTCCAGA GGGCCGAGCA GCGCCTCCTA GTGGCCGGTA CTCACACGTG
    GCGGCTGTGC TTGGTGGCAG TGTCCTGTTG GTGGCCGGGG GGTACAGTGG CCGGCCTCGT
    GGGGACTTGA TGGCCTACAA AGTGCCCCCC TTTGTGTTCC AGGCGCCTGC TCCAGATTAC
    CACTTGGACT ATTGCTCCAT GTACACGGAC CACAGCGTCT GCTCCCGAGA CCCCGAATGC
    AGTTGGTGTC AGGGGGCCTG CCAAGCCGCC CCACCTCCTG GGACTCCCTC AGGGGCTTGT
    CCGGCTGCCA GCTGCCTGGG CCTGAGCCGT CTCCTGGGTG ACTGCCAGGC CTGCCTGGCG
    TTCAGCAGCA GTGCGGCTCC CCCCCAGGGG CCCGGTGCCC TGGGCTGGTG CGTGCACAAT
    GAGAGCTGCC TCCCTCGGCC TGAGCAGGCC CGCTGCCGCG GGGAGCAGAT CTCCGGCACC
    GTGGGCTGGT GGGGGCCCGC TCCTGTCTTT GTGACCTCCC TGGAGGCCTG TGTCACCCAG
    AGCTTCCTGC CTGGCCTGCA CCTGCTCACC TTCCAGCAGC CACCCAACGC CTCCCAGCCT
    GACAAGGTCT TGATCGTCCG CAGCACGACC ATCACCCTGA CGCCGAGCTC AGAGACCGAC
    GTGTCCCTGG TCTACCGTGG CTTCATCCAC CCGCTGCTGC CGGGAGGCCC TGGGGGGTCT
    GGGGCTGAGG ATGTGGCCGT GTGGGCCCGG GCCCAGCGCC TGCATGTGCT GGCCCGGATG
    GCCCGGGGCC CGGACACAGA AAACATGGAG GAGGTGGGGC GCTGGGTGGC ACAGCAGGAG
    AAGGAGACGA GGCGGCTGCA GCGCCCAGGG TCAGCTCGCC TCTTCCCACT GCCCGGCCGG
    GGCCACAAGT ATGCAGTAGA GATCCGGGGC CAGCTCAATG GCTCAGCAGG CCCCGGGCAC
    AGTGAGCTCA CCCTGCTGTG GGACCGGACT GGCGTGCCAG GTGGCAGCGA GATCTCCTTC
    TTCTTCCTGG AGCCCTACCG CTCGTCGGCC TGCGCCTCCT ACTCCTCCTG CCTGGGCTGC
    CTGGCAGACC AGGGCTGCGG CTGGTGCCTG GCCAGCGCCA CCTGCCACCT GCGCCAGAGC
    GGCGCCCACT GTAGGGACGC TGGGGCCGGC GGGTCCCTGC TCGTGCTGGT GCCTGCCCTC
    TGCCCGCTCT GCGAGGAGCA TCGCGACTGC CACGCCTGCA CCCAGGACCC CTTCTGTGAG
    TGGCATCAGA GCACCAGCCG CAAGGGGGAC GCGGCGTGCA GCCGGCGCGG CCGGGGTCGG
    GGCGCCCTGA AGAGTCCGGA AGAGTGCCCC CCACTCTGCA GCCAGCGCCT GACCTGTGAC
    GACTGCCTGG CCAACTCGAG CCAGTGCGCC TGGTGCCAGT CCACCCGCAC CTGCTTCCTG
    TTCGCCGCCT ACCTGGCCCG GTACCCGCAC GGGGGCTGCC GAGGCTGGGA CGACAGCGTG
    CACTCGGAGC CTCGGTGCCG GAGCTGTGAC CGCTTCCCGA GCTGTCACGA GTGTCTGCAG
    AGCCATGAGT GTGGCTGGTG TGGAAACGAG GACAACCCCA CGCTGGGCCG TTGCCTCCAA
    GGGGACTTCT CAGGGCCTCT GAGTGGGGGG AACTGCTCCC TGTGGGTCGG GGAGGGCCTG
    GGGCTGTCTG TGGCCCTCCC CGCCCGCTGG GCGTATGCCC GCTGTCCGGA TGTAGACGAG
    TGCCGCCTGG GCCTGGCGCG GTGCCACCTG CGGGCGACCT GCCTGAACAC ACCCCTCAGC
    TACGAGTGTC ACTGCCAGAG GGGCTACCAG GGCGATGGCG TCACTTACTG CAACCGCACG
    TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC AGCGGCCCCC CAGACTTCAC CTGCGTGTGT
    GACCTGGGCT GGACATCAGA CCTGCCCCTT CCCACGCCGG CCCCCGGCCC CCCCGCCCCC
    CGCTGTTCGC GGGACTGTGG CTGCAACTTC CACAGCCACT GCCACAAGCG GGGGCCCGGC
    TTCTGCGATG AGTGCCAGGA CTGGACGTGG GGGGAGCACT GTGAGCGGTG CCGGCCTGGA
    AGCTTCGGGA ATGCCACAGG CTCCGGCGGC TGCCGGCCCT GCCAGTGCAA CGGGCATGGG
    GACCCTCGCC GAGGCCACTG TGACAACCTC AGCGGGCTCT GCTTCTGCCA GGACCACACC
    GAGGGTGCCC ACTGCCAGCT CTGCTCCCCT GGCTACTACG GGGACCCTCG GGCAGGTGGC
    TCCTGCTTCC GGGAGTGTGG GGGTCGTGCC CTCCTCACCA ACGTGTCCTC AGTGGCACTG
    GGCTCACGCC GGTTTGGGGG GCTCCTGCCT GCAGGTGGCG GGGCAGCAAG AGCGGGGCCT
    GGCCTGTCCT ACTGTGTGTG GGTTGTCTCG GCCACTGAGG TGCTGCAGCC CTGTGCCCCC
    GGGACCCTCT GTCCCCCGCT TACCCTCACC TTCTCCCCCG ACAGCAGCAC CCCGTGCACG
    CTGAGCTATG TCCTGGCCTT TGATGGATTC CCACGCTTCC TAGACACTGG TGTCGTCCAG
    TCGGACCGTA GCCTCATCGC TGCGTTTTGC GGCCAGCGGC GGGACCGGCC CCTCACTGTG
    CAGGCCCTGT CTGGGCTGCT GGTACTGCAC TGGGAGGCCA ATGGCTCCTC ATCCTGGGGC
    TTCAATGCCT CCGTGGGCTC TGCCCGCTGT GGGTCAGGGG GCCCCGGGAG CTGCCCTGTC
    CCCCAGGAGT GTGTGCCCCA GGATGGGGTC CCAGGTGCTG GACTCTGCCG GTGTCCCCAG
    GGCTGGGCTG GCCCGCACTG CCGCATGGCT GTGTGTCCTG AGAACTGTAA TGCCCACAAC
    GGGGCGGGGA CGTGTAATCA GAGCCTGGGC GTGTGCGTCT GTGCTGAGGG CTTTGGGGGT
    CCTGACTGTG CCACGAAGCT GGATGGTGGG CAGCTGGTCT GGGAGACCCT CATGGACAGC
    CGCCTCTCAG CTGACACTGC CAGCCGCTTC CTGCACCGCC TGGGGCACAC GATGGTGGAG
    GGACCTGATG CCACCTTGTG GATGTTTGGG GGCCTGGGCC TGCCCCAGGG GCTGCTGGGA
    AACCTGTATA GGTACTCAGT GAGTGAACGG CGGTGGACAC AGATGTTGGC AGGAGCCGAG
    GATGGGGGCC CAGGCCCCTC CCCTCGCTCT TTCCACGCTG CTGCCTACGT GCCTGCTGGC
    CGTGGGGCCA TGTACCTGCT GGGGGGGCTC ACAGCTGGGG GCATCGCCCG GGACTTCTGG
    GTCCTCAACC TCACCACCCT GCAGTGGCGG CAGGAGAAGG CCCCTCAGAC TGTGGAGCTG
    CCAGCCGTTG CTGGTCACAC GCTTACTGCC CGCCGAGGCC TATCTCTGCT CCTGGTGGGT
    GGTTACTCCC CGGAAAATGG CTTCAACCAG CAGCTGCTTG AGTACCAGCT GGCGACTGGC
    ACCTGGGTGT CCGGGGCTCA GAGCGGGACA CCCCCCACAG GTCTCTACGG TCACTCTGCT
    GTGTATCATG AGGCCACCGA CTCCCTCTAC GTGTTCGGGG GTTTCCGCTT CCACGTGGAG
    CTGGCAGCCC CGTCCCCTGA GCTCTACTCC CTGCACTGCC CTGACCGCAC CTGGAGCCTG
    CTGGCCCCTT CTCAGGGAGC AAAGCCCCGG CCCCGACTCT TCCACGCCTC GGCCTTGCTA
    GGGGACACCA TGGTGGTCCT CGGGGGGCGC TCAGACCCCG ACGAGTTCAG CAGCGACGTG
    CTGCTCTACC AGGTCAACTG CAACACCTGG CTCCTGCCCG ACCTCATCGG CCCAGCCTCT
    GTGGGACCGC CAATGGAAGA GTCTGTGGCT CACGCCGTGG CCGCGGTAGG GGGTCACCTG
    TTTATCTCAG GAGGCTTTGG GGGAGTGGCC CTGGGCCGCC TGCTGGCCCT GACCCTGCCC
    CCTGACCCCT GCCGCCTGCT GCCCTCGCCC GAAGCTTGTA ACCAGACTGG GGCCTGTACC
    TGGTGCCATG GAGCCTGCCT GTCTGGGGAC CAGGCCCACA GGCTGGGCTG TGGCGGCACC
    CCCTGCTCCC CTATGCCCCA CTCCCCCGAG GAATGTCGAC GTCTGCGGAC CTGCAGCGAG
    TGCCTGGCCC GCCATCCCCG GACCCTGCAG CCTGGAGATG GAGAGGCCTC TGCCCCACGC
    TGTAAGTGGT GTACCAACTG CCCCGAGGGT GCCTGCATTG GGCGCAACGG GTCCTGCACC
    TCAGAGAATG ACTGTCGCAT CAACCAGCGC GAGGTCTTCT GGGCCGGGAA CTGCTCGGAG
    GCTGTGTGCG GGGCGGCCGA CTGCGAGCAG TGCACCAGGG AGGGCAAATG CATGTGGACG
    CGGCAGTTCA AGAGGACGGG GGAGACCCGC CGCATCCTTT CGGTGCAGCC CACCTACGAC
    TGGACGTGCT TCAGCCACTC ACTGCTGAAC GTGTCCCCGA TGCCAGTGGA GTCATCGCCC
    CCGCTGCCCT GCCCCACACC CTGTCACCTC CTGCCCAACT GCACCTCCTG CCTGGACTCC
    AAGGGTGCTG ACGGGGGCTG GCAGCACTGC GTCTGGAGCA GCAGCCTCCA GCAGTGTCTG
    AGCCCCTCCT ATCTGCCCCT GCGGTGTATG GCCGGAGGCT GTGGGCGGCT GCTCCGCGGA
    CCCGAGAGCT GCTCCCTGGG CTGTGCTCAG GCAACCCAGT GTGCCCTGTG CCTGCGGCGC
    CCCCACTGTG GCTGGTGTAC CTGGGGGGGC CAGGATGGGG GCGGCCGCTG CCTGGAGGGT
    GGACTCAGTG GCCCCCGTGA CGGGCTGACG TGTGGGCGAC CTGGGGCCTC CTGGGCCTTT
    CTTTCCTGCC CCCCGGAGGA CGAATGTGCC AACGGGCACC ATGACTGCAA CGAGACCCAG
    AACTGCCACG ACCGGCCCCA CGGTTACGAG TGCAGCTGCA AGGCAGGCTA CACCATGGAC
    AACGTGACGG GGCTGTGCCG CCCAGTGTGC GCCCAGGGTT GCGTGAACGG CTCGTGTGTG
    GAGCCCGACC ACTGCCGCTG CCACTTCGGC TTCGTCGGCC GCAACTGTTC CACGGAGTGT
    CGCTGCAACC GCCACAGCGA CTGCGCCGGC GTGGGGGCCC GCGACCACTG CCTGCTCTGC
    CACAATCACA CCAAGGGCAG CCACTGTGAG CAGTGCCTCC CGCTGTTCGT GGGCTCAGCC
    GTAGGGGGAG GGACCTGCCG GCCCTGCCAC GCCTTTTGTC GCGGAAACAG CCACGTCTGC
    GTCTCCAGGA AGGAGCTTGA GATAGCCAGG AGGGAGCCGG AGAAGTACTC GCTGGATCCA
    GATGAGATTG AACACTGGGC GACAGAGGGT CCCAGCGAAG ATGAGGCTGT GTGTGTGAAC
    TGCCAGAATA ACAGCTATGG GGATAAGTGT GAGAGCTGCC TCCACGGCTA CTTCCTCCTC
    GACGGGAAAT GCACCAAATG CCAGTGTAAT GGCCACGCGG ACACATGTAA TGAGCAGGAT
    GGGACAGGCT GCCCATGTCA GAACAACACA GAGACAGGCA CGTGCCAGGG CAGCTCCCCC
    AGTGACCGCC GAGACTGCTA CAAATACCAG TGTGCCAAGT GCCGAGAGTC ATTCCACGGG
    AGCCCGCTAG GGGGCCAGCA GTGCTACCGC CTCATCTCCG TGGAACAGGA GTACTGCCTG
    GACCCCACGT CCCAAACCAA CTGTTTCCAC GAGCCCAAGC GCCGGGCTCT GGGTCCCGGA
    CGCACCGTTC TCTTTGGAGT GCAGCCTAAG TTCACCAACG TGGACATCCG CCTGACGCTG
    GACGTGACCT TTGGCGCCGT GGACCTCTAC GTCTCCACCT CCTATGACAC CTTCGTGGTC
    CGCGTGGCTC CCGACACTGG TGTCCACACC GTGCATATCC AGCCGCCACC ACCTCCCCCA
    CCCCCACCAC CCCCTGCTGA CGGCGGGCCC CGGGGACCTG GGGATCTCGG GGGACCTGGG
    GCTGGAGGTG GGCCGGCTAC CCCAGCAGAG CCGCGAGTGC GGGAGGTCTG GCCACGGGGC
    CTGATCACCT ACGTGACAGT GACAGAGCCG TCGGCGGTGC TGGTGGTCCG TGGCGTGAGG
    GACCGGCTGG TCATCACCTA CCCGCACGAG CACCACACCC TCAAGTCCAG CCGCTTCTAC
    CTGCTCCTGC TGGGTGTGGG AGACCCAAGT GGGCCCAGTG CCAACGGCTC AGCTGACTCG
    CAGGGCTTGC TGTTCTTCCG GCAGGACCAG GCCCACATTG ACCTGTTTGT CTTCTTCTCT
    GTCTTCTTCT CCTGCTTCTT CCTCTTCCTC TCGCTCTGTG TGCTGCTCTG GAAGGCCAAG
    CAGGCCCTGG ACCACCGGCA GGAACAGCGC CGGCACCTGC AGGAGATGAC CAAGATGGCC
    AGCCGCCCCT TCGCCAAGGT CACTGTCTGC TTCCCGCCAG ACCCTGCTGC CCCAGCCCCC
    ACCTGGAAGC CTGCCGGGCT CCCGCCACCC GCCTTCCGCC GCTCCGAGCC CTTCTTGGCA
    CCCTTGCTGC TGACCGGGGC CGGCGGGCCC TGGGGACCTG TAGGAGGGGG CTGCTGCCCA
    CCGGCCATCC CCACCACCAC TGCCGGCCTG CGAGCCGGGC CTATCACCCT TGAGCCCACC
    GAGGATGGCA TGGCCGGCGT GGCCACACTG CTGCTCCAAC TGCCTGGCGG GCCCCACGCA
    CCCAACGGCG CCTGCCTGGG GTCAGCGCTC GTCACACTGC GGCACCGGCT GCACGAATAC
    TGCGGCGGCG GCGGGGGCGC TGGGGGCAGT GGGCATGGGG CTGGTGCGGG CCGGAAGGGG
    CTGTTGAGCC AGGACAACCT CACCAGCATG TCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834867.1
    CDS473..8491
    Translation

    Target ORF information:

    RefSeq Version XM_024979099.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 8 (MEGF8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979099.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    ATGCTGCTGC ACCTCTTCAG CGATGCCAAC TACAACCTGC TGGGCTTCAA CGCCTCCTTC 
    CGCTTCTCCC TGTGCCCGGG TGGCTGCCGC AGCCATGGGC AGTGCCAGCC CCCAGGGGTG
    TGTGTCTGTG AGCCGGGCTG GGGGGGCCCC GACTGCAGCC TGCAGGAGTG CTCAGCCTAC
    TGCGGCAGCC ATGGCACCTG CACCTCGCCC CTGGGACCGT GCCGCTGTGA GCCTGGTTTC
    CTGGGACGCG CCTGTGACCT GCACCTGTGG GAGAACCAGG GGGCTGGCTG GTGGCACAAC
    GTGAGTGCCG GGGACCCCGC CTTCTCTGCC CGTATCGGGG CAGCCGGGGC CTTCCTGTCC
    CCACTGGGGC TGCTGGCTGT TTTTGGAGGC CAGGACCTCA ACCGCGCGCT GGGGGACCTC
    GTCCTGTACA ACTTCTCTGC CAACACCTGG GAGCGCTGGG ACCTGAGCCC TGCCCCGGCT
    GCCCGTCACT CCCACGTGGC TGTGGCTTGG GCCGGCTCCC TGGTGCTGAT GGGCGGTGAG
    CTGGCTGATG GCACCCTCAC CAGCGACATC TGGGCCTTTA GCCCGATGGG TGGCGGCCGC
    TGGGAGCTTC TGGCACCACC TGCCTCCAGT CCCTCGGGGC CGCCCGGCCT GGCAGGTCAT
    GCAGCAGCCC TGGTGGACAA CACGTGGCTC TATGTGTCTG GTGGCCGCAC CCAGCACGAC
    CTCTTCTCCT CCGGCCTCTT CCGTTTCCGC CTCGACGGCA CCAGTGGGGG CTATTGGGAG
    CAGGTGATGC CAGCAGGTGG GCGGCCCCCT GCCGCCACCG GCCATTCCAT GGTGTTCCAT
    GCCGCCTCCC GTGCCCTGCT GGTTCACGGT GGACACCGGC CCTCCACTGC CCGATTCTCT
    GTGCGGGTGA ACTCCACCGA GCTCTTCCAT GTGGATCGGC GCGTGTGGAC CACCCTGAAG
    GGCCGGGAGG GACTTCACGG CCCACGGGAG CGAGCCTTCC ACACGGCCAG CGTCCTGGGC
    AACTACATGC TGGTCTATGG GGGCAACGTG CACACCCACT ACCAGGAGGA AAAGTGCTAT
    GAAGACGGCA TCTTCTTCTA CCACCTGGGC TGCCATCAGT GGGTGTCAGG GGCTGAGCTT
    GCCCCTCCAG GAACTCCAGA GGGCCGAGCA GCGCCTCCTA GTGGCCGGTA CTCACACGTG
    GCGGCTGTGC TTGGTGGCAG TGTCCTGTTG GTGGCCGGGG GGTACAGTGG CCGGCCTCGT
    GGGGACTTGA TGGCCTACAA AGTGCCCCCC TTTGTGTTCC AGGCGCCTGC TCCAGATTAC
    CACTTGGACT ATTGCTCCAT GTACACGGAC CACAGCGTCT GCTCCCGAGA CCCCGAATGC
    AGTTGGTGTC AGGGGGCCTG CCAAGCCGCC CCACCTCCTG GGACTCCCTC AGGGGCTTGT
    CCGGCTGCCA GCTGCCTGGG CCTGAGCCGT CTCCTGGGTG ACTGCCAGGC CTGCCTGGCG
    TTCAGCAGCA GTGCGGCTCC CCCCCAGGGG CCCGGTGCCC TGGGCTGGTG CGTGCACAAT
    GAGAGCTGCC TCCCTCGGCC TGAGCAGGCC CGCTGCCGCG GGGAGCAGAT CTCCGGCACC
    GTGGGCTGGT GGGGGCCCGC TCCTGTCTTT GTGACCTCCC TGGAGGCCTG TGTCACCCAG
    AGCTTCCTGC CTGGCCTGCA CCTGCTCACC TTCCAGCAGC CACCCAACGC CTCCCAGCCT
    GACAAGGTCT TGATCGTCCG CAGCACGACC ATCACCCTGA CGCCGAGCTC AGAGACCGAC
    GTGTCCCTGG TCTACCGTGG CTTCATCCAC CCGCTGCTGC CGGGAGGCCC TGGGGGGTCT
    GGGGCTGAGG ATGTGGCCGT GTGGGCCCGG GCCCAGCGCC TGCATGTGCT GGCCCGGATG
    GCCCGGGGCC CGGACACAGA AAACATGGAG GAGGTGGGGC GCTGGGTGGC ACAGCAGGAG
    AAGGAGACGA GGCGGCTGCA GCGCCCAGGG TCAGCTCGCC TCTTCCCACT GCCCGGCCGG
    GGCCACAAGT ATGCAGTAGA GATCCGGGGC CAGCTCAATG GCTCAGCAGG CCCCGGGCAC
    AGTGAGCTCA CCCTGCTGTG GGACCGGACT GGCGTGCCAG GTGGCAGCGA GATCTCCTTC
    TTCTTCCTGG AGCCCTACCG CTCGTCGGCC TGCGCCTCCT ACTCCTCCTG CCTGGGCTGC
    CTGGCAGACC AGGGCTGCGG CTGGTGCCTG GCCAGCGCCA CCTGCCACCT GCGCCAGAGC
    GGCGCCCACT GTAGGGACGC TGGGGCCGGC GGGTCCCTGC TCGTGCTGGT GCCTGCCCTC
    TGCCCGCTCT GCGAGGAGCA TCGCGACTGC CACGCCTGCA CCCAGGACCC CTTCTGTGAG
    TGGCATCAGA GCACCAGCCG CAAGGGGGAC GCGGCGTGCA GCCGGCGCGG CCGGGGTCGG
    GGCGCCCTGA AGAGTCCGGA AGAGTGCCCC CCACTCTGCA GCCAGCGCCT GACCTGTGAC
    GACTGCCTGG CCAACTCGAG CCAGTGCGCC TGGTGCCAGT CCACCCGCAC CTGCTTCCTG
    TTCGCCGCCT ACCTGGCCCG GTACCCGCAC GGGGGCTGCC GAGGCTGGGA CGACAGCGTG
    CACTCGGAGC CTCGGTGCCG GAGCTGTGAC CGCTTCCCGA GCTGTCACGA GTGTCTGCAG
    AGCCATGAGT GTGGCTGGTG TGGAAACGAG GACAACCCCA CGCTGGGCCG TTGCCTCCAA
    GGGGACTTCT CAGGGCCTCT GAGTGGGGGG AACTGCTCCC TGTGGGTCGG GGAGGGCCTG
    GGGCTGTCTG TGGCCCTCCC CGCCCGCTGG GCGTATGCCC GCTGTCCGGA TGTAGACGAG
    TGCCGCCTGG GCCTGGCGCG GTGCCACCTG CGGGCGACCT GCCTGAACAC ACCCCTCAGC
    TACGAGTGTC ACTGCCAGAG GGGCTACCAG GGCGATGGCG TCACTTACTG CAACCGCACG
    TGCCTGGAGG ACTGTGGCCA CGGTGTGTGC AGCGGCCCCC CAGACTTCAC CTGCGTGTGT
    GACCTGGGCT GGACATCAGA CCTGCCCCTT CCCACGCCGG CCCCCGGCCC CCCCGCCCCC
    CGCTGTTCGC GGGACTGTGG CTGCAACTTC CACAGCCACT GCCACAAGCG GGGGCCCGGC
    TTCTGCGATG AGTGCCAGGA CTGGACGTGG GGGGAGCACT GTGAGCGGTG CCGGCCTGGA
    AGCTTCGGGA ATGCCACAGG CTCCGGCGGC TGCCGGCCCT GCCAGTGCAA CGGGCATGGG
    GACCCTCGCC GAGGCCACTG TGACAACCTC AGCGGGCTCT GCTTCTGCCA GGACCACACC
    GAGGGTGCCC ACTGCCAGCT CTGCTCCCCT GGCTACTACG GGGACCCTCG GGCAGGTGGC
    TCCTGCTTCC GGGAGTGTGG GGGTCGTGCC CTCCTCACCA ACGTGTCCTC AGTGGCACTG
    GGCTCACGCC GGTTTGGGGG GCTCCTGCCT GCAGGTGGCG GGGCAGCAAG AGCGGGGCCT
    GGCCTGTCCT ACTGTGTGTG GGTTGTCTCG GCCACTGAGG TGCTGCAGCC CTGTGCCCCC
    GGGACCCTCT GTCCCCCGCT TACCCTCACC TTCTCCCCCG ACAGCAGCAC CCCGTGCACG
    CTGAGCTATG TCCTGGCCTT TGATGGATTC CCACGCTTCC TAGACACTGG TGTCGTCCAG
    TCGGACCGTA GCCTCATCGC TGCGTTTTGC GGCCAGCGGC GGGACCGGCC CCTCACTGTG
    CAGGCCCTGT CTGGGCTGCT GGTACTGCAC TGGGAGGCCA ATGGCTCCTC ATCCTGGGGC
    TTCAATGCCT CCGTGGGCTC TGCCCGCTGT GGGTCAGGGG GCCCCGGGAG CTGCCCTGTC
    CCCCAGGAGT GTGTGCCCCA GGATGGGGTC CCAGGTGCTG GACTCTGCCG GTGTCCCCAG
    GGCTGGGCTG GCCCGCACTG CCGCATGGCT GTGTGTCCTG AGAACTGTAA TGCCCACAAC
    GGGGCGGGGA CGTGTAATCA GAGCCTGGGC GTGTGCGTCT GTGCTGAGGG CTTTGGGGGT
    CCTGACTGTG CCACGAAGCT GGATGGTGGG CAGCTGGTCT GGGAGACCCT CATGGACAGC
    CGCCTCTCAG CTGACACTGC CAGCCGCTTC CTGCACCGCC TGGGGCACAC GATGGTGGAG
    GGACCTGATG CCACCTTGTG GATGTTTGGG GGCCTGGGCC TGCCCCAGGG GCTGCTGGGA
    AACCTGTATA GGTACTCAGT GAGTGAACGG CGGTGGACAC AGATGTTGGC AGGAGCCGAG
    GATGGGGGCC CAGGCCCCTC CCCTCGCTCT TTCCACGCTG CTGCCTACGT GCCTGCTGGC
    CGTGGGGCCA TGTACCTGCT GGGGGGGCTC ACAGCTGGGG GCATCGCCCG GGACTTCTGG
    GTCCTCAACC TCACCACCCT GCAGTGGCGG CAGGAGAAGG CCCCTCAGAC TGTGGAGCTG
    CCAGCCGTTG CTGGTCACAC GCTTACTGCC CGCCGAGGCC TATCTCTGCT CCTGGTGGGT
    GGTTACTCCC CGGAAAATGG CTTCAACCAG CAGCTGCTTG AGTACCAGCT GGCGACTGGC
    ACCTGGGTGT CCGGGGCTCA GAGCGGGACA CCCCCCACAG GTCTCTACGG TCACTCTGCT
    GTGTATCATG AGGCCACCGA CTCCCTCTAC GTGTTCGGGG GTTTCCGCTT CCACGTGGAG
    CTGGCAGCCC CGTCCCCTGA GCTCTACTCC CTGCACTGCC CTGACCGCAC CTGGAGCCTG
    CTGGCCCCTT CTCAGGGAGC AAAGCCCCGG CCCCGACTCT TCCACGCCTC GGCCTTGCTA
    GGGGACACCA TGGTGGTCCT CGGGGGGCGC TCAGACCCCG ACGAGTTCAG CAGCGACGTG
    CTGCTCTACC AGGTCAACTG CAACACCTGG CTCCTGCCCG ACCTCATCGG CCCAGCCTCT
    GTGGGACCGC CAATGGAAGA GTCTGTGGCT CACGCCGTGG CCGCGGTAGG GGGTCACCTG
    TTTATCTCAG GAGGCTTTGG GGGAGTGGCC CTGGGCCGCC TGCTGGCCCT GACCCTGCCC
    CCTGACCCCT GCCGCCTGCT GCCCTCGCCC GAAGCTTGTA ACCAGACTGG GGCCTGTACC
    TGGTGCCATG GAGCCTGCCT GTCTGGGGAC CAGGCCCACA GGCTGGGCTG TGGCGGCACC
    CCCTGCTCCC CTATGCCCCA CTCCCCCGAG GAATGTCGAC GTCTGCGGAC CTGCAGCGAG
    TGCCTGGCCC GCCATCCCCG GACCCTGCAG CCTGGAGATG GAGAGGCCTC TGCCCCACGC
    TGTAAGTGGT GTACCAACTG CCCCGAGGGT GCCTGCATTG GGCGCAACGG GTCCTGCACC
    TCAGAGAATG ACTGTCGCAT CAACCAGCGC GAGGTCTTCT GGGCCGGGAA CTGCTCGGAG
    GCTGTGTGCG GGGCGGCCGA CTGCGAGCAG TGCACCAGGG AGGGCAAATG CATGTGGACG
    CGGCAGTTCA AGAGGACGGG GGAGACCCGC CGCATCCTTT CGGTGCAGCC CACCTACGAC
    TGGACGTGCT TCAGCCACTC ACTGCTGAAC GTGTCCCCGA TGCCAGTGGA GTCATCGCCC
    CCGCTGCCCT GCCCCACACC CTGTCACCTC CTGCCCAACT GCACCTCCTG CCTGGACTCC
    AAGGGTGCTG ACGGGGGCTG GCAGCACTGC GTCTGGAGCA GCAGCCTCCA GCAGTGTCTG
    AGCCCCTCCT ATCTGCCCCT GCGGTGTATG GCCGGAGGCT GTGGGCGGCT GCTCCGCGGA
    CCCGAGAGCT GCTCCCTGGG CTGTGCTCAG GCAACCCAGT GTGCCCTGTG CCTGCGGCGC
    CCCCACTGTG GCTGGTGTAC CTGGGGGGGC CAGGATGGGG GCGGCCGCTG CCTGGAGGGT
    GGACTCAGTG GCCCCCGTGA CGGGCTGACG TGTGGGCGAC CTGGGGCCTC CTGGGCCTTT
    CTTTCCTGCC CCCCGGAGGA CGAATGTGCC AACGGGCACC ATGACTGCAA CGAGACCCAG
    AACTGCCACG ACCGGCCCCA CGGTTACGAG TGCAGCTGCA AGGCAGGCTA CACCATGGAC
    AACGTGACGG GGCTGTGCCG CCCAGTGTGC GCCCAGGGTT GCGTGAACGG CTCGTGTGTG
    GAGCCCGACC ACTGCCGCTG CCACTTCGGC TTCGTCGGCC GCAACTGTTC CACGGAGTGT
    CGCTGCAACC GCCACAGCGA CTGCGCCGGC GTGGGGGCCC GCGACCACTG CCTGCTCTGC
    CACAATCACA CCAAGGGCAG CCACTGTGAG CAGTGCCTCC CGCTGTTCGT GGGCTCAGCC
    GTAGGGGGAG GGACCTGCCG GCCCTGCCAC GCCTTTTGTC GCGGAAACAG CCACGTCTGC
    GTCTCCAGGA AGGAGCTTGA GATAGCCAGG AGGGAGCCGG AGAAGTACTC GCTGGATCCA
    GATGAGATTG AACACTGGGC GACAGAGGGT CCCAGCGAAG ATGAGGCTGT GTGTGTGAAC
    TGCCAGAATA ACAGCTATGG GGATAAGTGT GAGAGCTGCC TCCACGGCTA CTTCCTCCTC
    GACGGGAAAT GCACCAAATG CCAGTGTAAT GGCCACGCGG ACACATGTAA TGAGCAGGAT
    GGGACAGGCT GCCCATGTCA GAACAACACA GAGACAGGCA CGTGCCAGGG CAGCTCCCCC
    AGTGACCGCC GAGACTGCTA CAAATACCAG TGTGCCAAGT GCCGAGAGTC ATTCCACGGG
    AGCCCGCTAG GGGGCCAGCA GTGCTACCGC CTCATCTCCG TGGAACAGGA GTACTGCCTG
    GACCCCACGT CCCAAACCAA CTGTTTCCAC GAGCCCAAGC GCCGGGCTCT GGGTCCCGGA
    CGCACCGTTC TCTTTGGAGT GCAGCCTAAG TTCACCAACG TGGACATCCG CCTGACGCTG
    GACGTGACCT TTGGCGCCGT GGACCTCTAC GTCTCCACCT CCTATGACAC CTTCGTGGTC
    CGCGTGGCTC CCGACACTGG TGTCCACACC GTGCATATCC AGCCGCCACC ACCTCCCCCA
    CCCCCACCAC CCCCTGCTGA CGGCGGGCCC CGGGGACCTG GGGATCTCGG GGGACCTGGG
    GCTGGAGGTG GGCCGGCTAC CCCAGCAGAG CCGCGAGTGC GGGAGGTCTG GCCACGGGGC
    CTGATCACCT ACGTGACAGT GACAGAGCCG TCGGCGGTGC TGGTGGTCCG TGGCGTGAGG
    GACCGGCTGG TCATCACCTA CCCGCACGAG CACCACACCC TCAAGTCCAG CCGCTTCTAC
    CTGCTCCTGC TGGGTGTGGG AGACCCAAGT GGGCCCAGTG CCAACGGCTC AGCTGACTCG
    CAGGGCTTGC TGTTCTTCCG GCAGGACCAG GCCCACATTG ACCTGTTTGT CTTCTTCTCT
    GTCTTCTTCT CCTGCTTCTT CCTCTTCCTC TCGCTCTGTG TGCTGCTCTG GAAGGCCAAG
    CAGGCCCTGG ACCACCGGCA GGAACAGCGC CGGCACCTGC AGGAGATGAC CAAGATGGCC
    AGCCGCCCCT TCGCCAAGGT CACTGTCTGC TTCCCGCCAG ACCCTGCTGC CCCAGCCCCC
    ACCTGGAAGC CTGCCGGGCT CCCGCCACCC GCCTTCCGCC GCTCCGAGCC CTTCTTGGCA
    CCCTTGCTGC TGACCGGGGC CGGCGGGCCC TGGGGACCTG TAGGAGGGGG CTGCTGCCCA
    CCGGCCATCC CCACCACCAC TGCCGGCCTG CGAGCCGGGC CTATCACCCT TGAGCCCACC
    GAGGATGGCA TGGCCGGCGT GGCCACACTG CTGCTCCAAC TGCCTGGCGG GCCCCACGCA
    CCCAACGGCG CCTGCCTGGG GTCAGCGCTC GTCACACTGC GGCACCGGCT GCACGAATAC
    TGCGGCGGCG GCGGGGGCGC TGGGGGCAGT GGGCATGGGG CTGGTGCGGG CCGGAAGGGG
    CTGTTGAGCC AGGACAACCT CACCAGCATG TCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93525
    Clone ID Related Accession (Same CDS sequence) XM_015467508.1 , XM_015458238.1
    Accession Version XM_015467508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA
    AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG
    CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC
    CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT
    CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG
    CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG
    GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC
    GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC
    CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA
    GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA
    GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC
    CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG
    GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG
    GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG
    TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC
    CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322994.1
    CDS1064..9193
    Translation

    Target ORF information:

    RefSeq Version XM_015467508.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA
    AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG
    CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC
    CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT
    CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG
    CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG
    GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC
    GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC
    CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA
    GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA
    GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC
    CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG
    GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG
    GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG
    TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC
    CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93525
    Clone ID Related Accession (Same CDS sequence) XM_015467508.1 , XM_015458238.1
    Accession Version XM_015458238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product multiple epidermal growth factor-like domains protein 8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA
    AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG
    CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC
    CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT
    CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG
    CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG
    GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC
    GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC
    CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA
    GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA
    GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC
    CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG
    GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG
    GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG
    TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC
    CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015313724.1
    CDS1064..9193
    Translation

    Target ORF information:

    RefSeq Version XM_015458238.1
    Organism Bos taurus(cattle)
    Definition Bos taurus multiple EGF like domains 8 (MEGF8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    ATGGCCCTGG GTCGGGCTCT GGCTGTGGCG CTGGCTGTAT CATTGGCCGT GCTGGGGCCA 
    CTGTCTCCTG GAGCCCAGGC AGGGGACTGC AAGGGGCAGC GGCAAGTGCT GCGGGAGGCT
    CCAGGCTTCG TGACAGATGG TGCGGGCAAC TACAGCGTCA ATGGCAATTG CGAATGGCTC
    ATCGAGGCCC CGAGCTCCCA GCACCGGATC CTACTGGACT TCCTTTTCCT GGACACAGAA
    TGCACTTATG ACTACCTGTT CGTGTATGAC GGTGACTCCC CCCGCGGGCC CCTGCTGGCC
    AGTCTGAGTG GGAGCACACG GCCTCCGCCC ATCGAGGCTT CCTCGGGCAA GATGCTGCTG
    CACCTCTTCA GCGATGCCAA CTACAACCTG CTGGGCTTCA ACGCCTCCTT CCGCTTCTCC
    CTGTGCCCGG GTGGCTGCCG CAGCCATGGG CAGTGCCAGC CCCCAGGGGT GTGTGTCTGT
    GAGCCGGGCT GGGGGGGCCC CGACTGCAGC CTGCAGGAGT GCTCAGCCTA CTGCGGCAGC
    CATGGCACCT GCACCTCGCC CCTGGGACCG TGCCGCTGTG AGCCTGGTTT CCTGGGACGC
    GCCTGTGACC TGCACCTGTG GGAGAACCAG GGGGCTGGCT GGTGGCACAA CGTGAGTGCC
    GGGGACCCCG CCTTCTCTGC CCGTATCGGG GCAGCCGGGG CCTTCCTGTC CCCACTGGGG
    CTGCTGGCTG TTTTTGGAGG CCAGGACCTC AACCGCGCGC TGGGGGACCT CGTCCTGTAC
    AACTTCTCTG CCAACACCTG GGAGCGCTGG GACCTGAGCC CTGCCCCGGC TGCCCGTCAC
    TCCCACGTGG CTGTGGCTTG GGCCGGCTCC CTGGTGCTGA TGGGCGGTGA GCTGGCTGAT
    GGCACCCTCA CCAGCGACAT CTGGGCCTTT AGCCCGATGG GTGGCGGCCG CTGGGAGCTT
    CTGGCACCAC CTGCCTCCAG TCCCTCGGGG CCGCCCGGCC TGGCAGGTCA TGCAGCAGCC
    CTGGTGGACA ACACGTGGCT CTATGTGTCT GGTGGCCGCA CCCAGCACGA CCTCTTCTCC
    TCCGGCCTCT TCCGTTTCCG CCTCGACGGC ACCAGTGGGG GCTATTGGGA GCAGGTGATG
    CCAGCAGGTG GGCGGCCCCC TGCCGCCACC GGCCATTCCA TGGTGTTCCA TGCCGCCTCC
    CGTGCCCTGC TGGTTCACGG TGGACACCGG CCCTCCACTG CCCGATTCTC TGTGCGGGTG
    AACTCCACCG AGCTCTTCCA TGTGGATCGG CGCGTGTGGA CCACCCTGAA GGGCCGGGAG
    GGACTTCACG GCCCACGGGA GCGAGCCTTC CACACGGCCA GCGTCCTGGG CAACTACATG
    CTGGTCTATG GGGGCAACGT GCACACCCAC TACCAGGAGG AAAAGTGCTA TGAAGACGGC
    ATCTTCTTCT ACCACCTGGG CTGCCATCAG TGGGTGTCAG GGGCTGAGCT TGCCCCTCCA
    GGAACTCCAG AGGGCCGAGC AGCGCCTCCT AGTGGCCGGT ACTCACACGT GGCGGCTGTG
    CTTGGTGGCA GTGTCCTGTT GGTGGCCGGG GGGTACAGTG GCCGGCCTCG TGGGGACTTG
    ATGGCCTACA AAGTGCCCCC CTTTGTGTTC CAGGCGCCTG CTCCAGATTA CCACTTGGAC
    TATTGCTCCA TGTACACGGA CCACAGCGTC TGCTCCCGAG ACCCCGAATG CAGTTGGTGT
    CAGGGGGCCT GCCAAGCCGC CCCACCTCCT GGGACTCCCT CAGGGGCTTG TCCGGCTGCC
    AGCTGCCTGG GCCTGAGCCG TCTCCTGGGT GACTGCCAGG CCTGCCTGGC GTTCAGCAGC
    AGTGCGGCTC CCCCCCAGGG GCCCGGTGCC CTGGGCTGGT GCGTGCACAA TGAGAGCTGC
    CTCCCTCGGC CTGGTAAGTG TCCACAGCAC CCCGTGCACG ACACTCGTGA CAGCTCGGGA
    AGCGGTCACA GCTCCGGCAC CGAGGCTCCG AGTGCACGCT GTGGGGAGGC GGGTGTGAGG
    CCGCCCGAGC TACCCCCGCC CCGCCCCACC TCCACCCTCC CCTTCCGCGC CCCTGCCGCC
    CGCCCGCACC TGTCGTCCCA GCCTCGGCAG CCCCCGTGCG GGTACCGGGC CAGCGGAGCT
    CACTGGCTGC AGAGTGGGGG GCACTCTTCC GGACTCTTCA GGGCGCCCCG ACCCCGGCCG
    CGCCGGCTGC ACGCCGCGTC CCCCTTGCGG CTGCCTGGTG CGCACCTGGG TGCAGGCGTG
    GCAGTCGCGA TGCTCCTCGC AGAGCGGGCA GAGGGCAGGC ACCAGCACGA GCAGGGACCC
    GCCGGCCCCA GCGTCCCTAC AGTGGGCGCC GCTCTGGCGC AGGTGGCAGG TGGCGCTGGC
    CAGGCACCAG CCGCAGCCCT GGTCTGCCAG GCAGCCCAGG CAGGAGGAGT AGGAGGCGCA
    GGCCGACGAG CGGTAGGGCT CCAGGAAGAA GAAGGAGATC TCCTGGGGGT GAGGGTGTCA
    GAGGAGGGTG AGGGCTCCAT GCTCCAGGTC AAGCCTGCTG AGTGTCCCAG GCATTCTTAC
    CATGTTTTCT GTGTCCGGGC CCCGGGCCAT CCGGGCCAGC ACATGCAGGC GCTGGGCCCG
    GGCCCACACG GCCACATCCT CAGCCCCAGA CCCCCCAGGG CCTCCCGGCA GCAGCGGGTG
    GATGAAGCCA CGTCTGCCTC TCTTCCATTT CTCCTGTCTG TCTCCATATT CATCTTTGTG
    TCTCTCTGGT TTATCTCCTC TCTCCCTGTC CCCGTCTCTC TGTCTCTCTC CTTACTTTGC
    CCCTCCACCC CAATCGCCTT CTTCAACCCC ACTCCCCGCA GTTGCCTCCA AGGGGACTTC
    TCAGGGCCTC TGAGTGGGGG GAACTGCTCC CTGTGGGTCG GGGAGGGCCT GGGGCTGTCT
    GTGGCCCTCC CCGCCCGCTG GGCGTATGCC CGCTGTCCGG ATGTAGACGA GTGCCGCCTG
    GGCCTGGCGC GGTGCCACCT GCGGGCGACC TGCCTGAACA CACCCCTCAG CTACGAGTGT
    CACTGCCAGA GGGGCTACCA GGGCGATGGC GTCACTTACT GCAACCGCAC GTGCCTGGAG
    GACTGTGGCC ACGGTGTGTG CAGCGGCCCC CCAGACTTCA CCTGCGTGTG TGACCTGGGC
    TGGACATCAG ACCTGCCCCT TCCCACGCCG GCCCCCGGCC CCCCCGCCCC CCGCTGTTCG
    CGGGACTGTG GCTGCAACTT CCACAGCCAC TGCCACAAGC GGGGGCCCGG CTTCTGCGAT
    GAGTGCCAGG ACTGGACGTG GGGGGAGCAC TGTGAGCGGT GCCGGCCTGG AAGCTTCGGG
    AATGCCACAG GCTCCGGCGG CTGCCGGCCC TGCCAGTGCA ACGGGCATGG GGACCCTCGC
    CGAGGCCACT GTGACAACCT CAGCGGGCTC TGCTTCTGCC AGGACCACAC CGAGGGTGCC
    CACTGCCAGC TCTGCTCCCC TGGCTACTAC GGGGACCCTC GGGCAGGTGG CTCCTGCTTC
    CGGGAGTGTG GGGGTCGTGC CCTCCTCACC AACGTGTCCT CAGTGGCACT GGGCTCACGC
    CGGTTTGGGG GGCTCCTGCC TGCAGGTGGC GGGGCAGCAA GAGCGGGGCC TGGCCTGTCC
    TACTGTGTGT GGGTTGTCTC GGCCACTGAG GTGCTGCAGC CCTGTGCCCC CGGGACCCTC
    TGTCCCCCGC TTACCCTCAC CTTCTCCCCC GACAGCAGCA CCCCGTGCAC GCTGAGCTAT
    GTCCTGGCCT TTGATGGATT CCCACGCTTC CTAGACACTG GTGTCGTCCA GTCGGACCGT
    AGCCTCATCG CTGCGTTTTG CGGCCAGCGG CGGGACCGGC CCCTCACTGT GCAGGCCCTG
    TCTGGGCTGC TGGTACTGCA CTGGGAGGCC AATGGCTCCT CATCCTGGGG CTTCAATGCC
    TCCGTGGGCT CTGCCCGCTG TGGGTCAGGG GGCCCCGGGA GCTGCCCTGT CCCCCAGGAG
    TGTGTGCCCC AGGATGGGGT CCCAGGTGCT GGACTCTGCC GGTGTCCCCA GGGCTGGGCT
    GGCCCGCACT GCCGCATGGC TGTGTGTCCT GAGAACTGTA ATGCCCACAA CGGGGCGGGG
    ACGTGTAATC AGAGCCTGGG CGTGTGCGTC TGTGCTGAGG GCTTTGGGGG TCCTGACTGT
    GCCACGAAGC TGGATGGTGG GCAGCTGGTC TGGGAGACCC TCATGGACAG CCGCCTCTCA
    GCTGACACTG CCAGCCGCTT CCTGCACCGC CTGGGGCACA CGATGGTGGA GGGACCTGAT
    GCCACCTTGT GGATGTTTGG GGGCCTGGGC CTGCCCCAGG GGCTGCTGGG AAACCTGTAT
    AGGTACTCAG TGAGTGAACG GCGGTGGACA CAGATGTTGG CAGGAGCCGA GGATGGGGGC
    CCAGGCCCCT CCCCTCGCTC TTTCCACTCT GCTGCCTACG TGCCTGCTGG CCGTGGGGCC
    ATGTACCTGC TGGGGGGGCT CACAGCTGGG GGCATCGCCC GGGACTTCTG GGTCCTCAAC
    CTCACCACCC TGCAGTGGCG GCAGGAGAAG GCCCCTCAGA CTGTGGAGCT GCCAGCCGTT
    GCTGGTCACA CGCTTACTGC CCGCCGAGGC CTATCTCTGC TCCTGGTGGG TGGTTACTCC
    CCGGAAAATG GCTTCAACCA GCAGCTGCTT GAGTACCAGC TGGCGACTGG CACCTGGGTG
    TCCGGGGCTC AGAGCGGGAC ACCCCCCACA GGTCTCTACG GTCACTCTGC TGTGTATCAT
    GAGGCCACCG ACTCCCTCTA CGTGTTCGGG GGTTTCCGCT TCCACGTGGA GCTGGCAGCC
    CCGTCCCCTG AGCTCTACTC CCTGCACTGC CCTGACCGCA CCTGGAGCCT GCTGGCCCCT
    TCTCAGGGAG CAAAGCCCCG GCCCCGACTC TTCCACGCCT CGGCCTTGCT AGGGGACACC
    ATGGTGGTCC TCGGGGGGCG CTCAGACCCC GACGAGTTCA GCAGCGACGT GCTGCTCTAC
    CAGGTCAACT GCAACACCTG GCTCCTGCCC GACCTCATCG GCCCAGCCTC TGTGGGACCG
    CCAATGGAAG AGTCTGTGGC TCACGCCGTG GCCGCGGTAG GGGGTCACCT GTTTATCTCA
    GGAGGCTTTG GGGGAGTGGC CCTGGGCCGC CTGCTGGCCC TGACCCTGCC CCCTGACCCC
    TGCCGCCTGC TGCCCTCGCC CGAAGCTTGT AACCAGACTG GGGCCTGTAC CTGGTGCCAT
    GGAGCCTGCC TGTCTGGGGA CCAGGCCCAC AGGCTGGGCT GTGGCGGCAC CCCCTGCTCC
    CCTATGCCCC ACTCCCCCGA GGAATGTCGA CGTCTGCGGA CCTGCAGCGA GTGCCTGGCC
    CGCCATCCCC GGACCCTGCA GCCTGGAGAT GGAGAGGCCT CTGCCCCACG CTGTAAGTGG
    TGTACCAACT GCCCCGAGGG TGCCTGCATT GGGCGCAACG GGTCCTGCAC CTCAGAGAAT
    GACTGTCGCA TCAACCAGCG CGAGGTCTTC TGGGCCGGGA ACTGCTCGGA GGCTGTGTGC
    GGGGCGGCCG ACTGCGAGCA GTGCACCAGG GAGGGCAAAT GCATGTGGAC GCGGCAGTTC
    AAGAGGACGG GGGAGACCCG CCGCATCCTT TCGGTGCAGC CCACCTACGA CTGGACGTGC
    TTCAGCCACT CACTGCTGAA CGTGTCCCCG ATGCCAGTGG AGTCATCGCC CCCGCTGCCC
    TGCCCCACAC CCTGTCACCT CCTGCCCAAC TGCACCTCCT GCCTGGACTC CAAGGGTGCT
    GACGGGGGCT GGCAGCACTG CGTCTGGAGC AGCAGCCTCC AGCAGTGTCT GAGCCCCTCC
    TATCTGCCCC TGCGGTGTAT GGCCGGAGGC TGTGGGCGGC TGCTCCGCGG ACCCGAGAGC
    TGCTCCCTGG GCTGTGCTCA GGCAACCCAG TGTGCCCTGT GCCTGCGGCG CCCCCACTGT
    GGCTGGTGTA CCTGGGGGGG CCAGGATGGG GGCGGCCGCT GCCTGGAGGG TGGACTCAGT
    GGCCCCCGTG ACGGGCTGAC GTGTGGGCGA CCTGGGGCCT CCTGGGCCTT TCTTTCCTGC
    CCCCCGGAGG ACGAATGTGC CAACGGGCAC CATGACTGCA ACGAGACCCA GAACTGCCAC
    GACCGGCCCC ACGGTTACGA GTGCAGCTGC AAGGCAGGCT ACACCATGGA CAACGTGACG
    GGGCTGTGCC GCCCAGTGTG CGCCCAGGGT TGCGTGAACG GCTCGTGTGT GGAGCCCGAC
    CACTGCCGCT GCCACTTCGG CTTCGTCGGC CGCAACTGTT CCACGGAGTG TCGCTGCAAC
    CGCCACAGCG ACTGCGCCGG CGTGGGGGCC CGCGACCACT GCCTGCTCTG CCACAATCAC
    ACCAAGGGCA GCCACTGTGA GCAGTGCCTC CCGCTGTTCG TGGGCTCAGC CGTAGGGGGA
    GGGACCTGCC GGCCCTGCCA CGCCTTTTGT CGCGGAAACA GCCACGTCTG CGTCTCCAGG
    AAGGAGCTTG AGATAGCCAG GAGGGAGCCG GAGAAGTACT CGCTGGATCC AGATGAGATT
    GAACACTGGG CGACAGAGGG TCCCAGCGAA GATGAGGCTG TGTGTGTGAA CTGCCAGAAT
    AACAGCTATG GGGATAAGTG TGAGAGCTGC CTCCACGGCT ACTTCCTCCT CGACGGGAAA
    TGCACCAAAT GCCAGTGTAA TGGCCACGCG GACACATGTA ATGAGCAGGA TGGGACAGGC
    TGCCCATGTC AGAACAACAC AGAGACAGGC ACGTGCCAGG GCAGCTCCCC CAGTGACCGC
    CGAGACTGCT ACAAATACCA GTGTGCCAAG TGCCGAGAGT CATTCCACGG GAGCCCGCTA
    GGGGGCCAGC AGTGCTACCG CCTCATCTCC GTGGAACAGG AGTACTGCCT GGACCCCACG
    TCCCAAACCA ACTGTTTCCA CGAGCCCAAG CGCCGGGCTC TGGGTCCCGG ACGCACCGTT
    CTCTTTGGAG TGCAGCCTAA GTTCACCAAC GTGGACATCC GCCTGACGCT GGACGTGACC
    TTTGGCGCCG TGGACCTCTA CGTCTCCACC TCCTATGACA CCTTCGTGGT CCGCGTGGCT
    CCCGACACTG GTGTCCACAC CGTGCATATC CAGCCGCCAC CACCTCCCCC ACCCCCACCA
    CCCCCTGCTG ACGGCGGGCC CCGGGGACCT GGGGATCTCG GGGGACCTGG GGCTGGAGGT
    GGGCCGGCTA CCCCAGCAGA GCCGCGAGTG CGGGAGGTCT GGCCACGGGG CCTGATCACC
    TACGTGACAG TGACAGAGCC GTCGGCGGTG CTGGTGGTCC GTGGCGTGAG GGACCGGCTG
    GTCATCACCT ACCCGCACGA GCACCACACC CTCAAGTCCA GCCGCTTCTA CCTGCTCCTG
    CTGGGTGTGG GAGACCCAAG TGGGCCCAGT GCCAACGGCT CAGCTGACTC GCAGGGCTTG
    CTGTTCTTCC GGCAGGACCA GGCCCACATT GACCTGTTTG TCTTCTTCTC TGTCTTCTTC
    TCCTGCTTCT TCCTCTTCCT CTCGCTCTGT GTGCTGCTCT GGAAGGCCAA GCAGGCCCTG
    GACCACCGGC AGGAACAGCG CCGGCACCTG CAGGAGATGA CCAAGATGGC CAGCCGCCCC
    TTCGCCAAGG TCACTGTCTG CTTCCCGCCA GACCCTGCTG CCCCAGCCCC CACCTGGAAG
    CCTGCCGGGC TCCCGCCACC CGCCTTCCGC CGCTCCGAGC CCTTCTTGGC ACCCTTGCTG
    CTGACCGGGG CCGGCGGGCC CTGGGGACCT GTAGGAGGGG GCTGCTGCCC ACCGGCCATC
    CCCACCACCA CTGCCGGCCT GCGAGCCGGG CCTATCACCC TTGAGCCCAC CGAGGATGGC
    ATGGCCGGCG TGGCCACACT GCTGCTCCAA CTGCCTGGCG GGCCCCACGC ACCCAACGGC
    GCCTGCCTGG GGTCAGCGCT CGTCACACTG CGGCACCGGC TGCACGAATA CTGCGGCGGC
    GGCGGGGGCG CTGGGGGCAG TGGGCATGGG GCTGGTGCGG GCCGGAAGGG GCTGTTGAGC
    CAGGACAACC TCACCAGCAT GTCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.