KDM2B cDNA ORF clone, Bos taurus(cattle)

The following KDM2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa09015 XM_005195033.3
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OBa09015 XM_005217980.4
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OBa09017 XM_005195035.3
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OBa09017 XM_005217982.2
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OBa09018 XM_005195036.3
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa09018 XM_005217983.4
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa09020 XM_005195038.3
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa09020 XM_005217985.2
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa09014 XM_024977708.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa224604 XM_024977704.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa224605 XM_024977705.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa224606 XM_024977706.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa224607 XM_024977707.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa224608 XM_024977709.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa224608 XM_010814030.3
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa224609 XM_024977710.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa224610 XM_024977711.1
Latest version!
Bos taurus lysine demethylase 2B (KDM2B), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa55412 XM_010814030.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa55412 XM_010823384.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa55413 XM_010814031.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa55413 XM_010823385.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa55414 XM_010823386.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa55414 XM_010814032.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa55415 XM_010814033.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa55415 XM_010823387.2
Latest version!
Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OBa09015 XM_005217980.3 Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $797.30
$1139.00
OBa09018 XM_005217983.3 Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa09015
    Clone ID Related Accession (Same CDS sequence) XM_005195033.3 , XM_005217980.3 , XM_005217980.4
    Accession Version XM_005195033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195090.1
    CDS241..4272
    Translation

    Target ORF information:

    RefSeq Version XM_005195033.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09015
    Clone ID Related Accession (Same CDS sequence) XM_005195033.3 , XM_005217980.3 , XM_005217980.4
    Accession Version XM_005217980.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218037.1
    CDS241..4272
    Translation

    Target ORF information:

    RefSeq Version XM_005217980.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217980.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09015
    Clone ID Related Accession (Same CDS sequence) XM_005195033.3 , XM_005217980.3 , XM_005217980.4
    Accession Version XM_005217980.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217980.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218037.1
    CDS280..4311
    Translation

    Target ORF information:

    RefSeq Version XM_005217980.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217980.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09017
    Clone ID Related Accession (Same CDS sequence) XM_005217982.2 , XM_005195035.3
    Accession Version XM_005217982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005217982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    GGCACTGTGC CTCCAGCCCC AGGGAGGGAG TGGCGATCTC CCTGCTCCCC GGGGACAGCA
    GGCTCAGCGG GAGGATCAAG GCCTCCGGCC CAGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218039.1
    CDS16..4029
    Translation

    Target ORF information:

    RefSeq Version XM_005217982.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    GGCACTGTGC CTCCAGCCCC AGGGAGGGAG TGGCGATCTC CCTGCTCCCC GGGGACAGCA
    GGCTCAGCGG GAGGATCAAG GCCTCCGGCC CAGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09017
    Clone ID Related Accession (Same CDS sequence) XM_005217982.2 , XM_005195035.3
    Accession Version XM_005195035.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195035.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    GGCACTGTGC CTCCAGCCCC AGGGAGGGAG TGGCGATCTC CCTGCTCCCC GGGGACAGCA
    GGCTCAGCGG GAGGATCAAG GCCTCCGGCC CAGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195092.1
    CDS16..4029
    Translation

    Target ORF information:

    RefSeq Version XM_005195035.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195035.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    GGCACTGTGC CTCCAGCCCC AGGGAGGGAG TGGCGATCTC CCTGCTCCCC GGGGACAGCA
    GGCTCAGCGG GAGGATCAAG GCCTCCGGCC CAGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09018
    Clone ID Related Accession (Same CDS sequence) XM_005217983.3 , XM_005195036.3 , XM_005217983.4
    Accession Version XM_005217983.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218040.1
    CDS239..4168
    Translation

    Target ORF information:

    RefSeq Version XM_005217983.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217983.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09018
    Clone ID Related Accession (Same CDS sequence) XM_005217983.3 , XM_005195036.3 , XM_005217983.4
    Accession Version XM_005195036.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195093.1
    CDS239..4168
    Translation

    Target ORF information:

    RefSeq Version XM_005195036.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195036.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09018
    Clone ID Related Accession (Same CDS sequence) XM_005217983.3 , XM_005195036.3 , XM_005217983.4
    Accession Version XM_005217983.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217983.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218040.1
    CDS276..4205
    Translation

    Target ORF information:

    RefSeq Version XM_005217983.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217983.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAGGCAG AGAAAGACTC TGGAAGAAGA TTGCGCCCGA TTGACCGCCA AAGATACGAC 
    GAGAACGAGG ACTTGTCGGA CGTGGAGGAG ATCGTCAGCG CCCGCGGCTT CAGCCTGGAG
    GAAAAGCTGC GCAGCCAGCT GTACCAGGGG GATTTCGTGC ACGCCATGGA GGGCAAAGAT
    TTCAACTATG AGTATGTGCA GAGAGAAGCT CTCAGAGTTC CCCTGGTATT TCGAGAAAAG
    GATGGCCTGG GAATTAAGAT GCCTGACCCT GATTTCACAG TCCGAGATGT CAAACTCCTA
    GTGGGTAGCC GACGGCTGGT GGACGTGATG GACGTGAACA CCCAGAAAGG CACGGAGATG
    AGCATGTCCC AGTTCGTACG GTACTACGAG ACGCCCGAGG CCCAGCGGGA CAAGCTGTAC
    AACGTCATCA GCCTTGAGTT CAGCCACACC AAGCTGGAGC ACTTGGTCAA GCGTCCCACC
    GTGGTAGACC TGGTGGACTG GGTGGACAAC ATGTGGCCTC AGCACTTGAA GGAGAAGCAG
    ACGGAAGCCA CGAATGCCAT TGCAGAGATG AAGTACCCGA AAGTGAAGAA GTACTGTCTG
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09020
    Clone ID Related Accession (Same CDS sequence) XM_005217985.2 , XM_005195038.3
    Accession Version XM_005217985.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005217985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    CCAGTGCTGC CTCACACCGC TGTGTGCCTG GTGTGTGGCG AGGCAGGAAA AGAGGACACA
    GTGGAAGAGG AAGAAGGCAA ATTTAACCTC ATGCTCATGG AATGCTCCAT CTGCAATGAA
    ATCATCCACC CTGGATGCCT TAAGATTAAG GAGTCAGAGG GCGTGGTCAA CGATGAGCTT
    CCAAACTGCT GGGAGTGTCC CAAGTGTAAC CACGCCGGCA AGACCGGGAA ACAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218042.1
    CDS16..3927
    Translation

    Target ORF information:

    RefSeq Version XM_005217985.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217985.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    CCAGTGCTGC CTCACACCGC TGTGTGCCTG GTGTGTGGCG AGGCAGGAAA AGAGGACACA
    GTGGAAGAGG AAGAAGGCAA ATTTAACCTC ATGCTCATGG AATGCTCCAT CTGCAATGAA
    ATCATCCACC CTGGATGCCT TAAGATTAAG GAGTCAGAGG GCGTGGTCAA CGATGAGCTT
    CCAAACTGCT GGGAGTGTCC CAAGTGTAAC CACGCCGGCA AGACCGGGAA ACAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09020
    Clone ID Related Accession (Same CDS sequence) XM_005217985.2 , XM_005195038.3
    Accession Version XM_005195038.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    CCAGTGCTGC CTCACACCGC TGTGTGCCTG GTGTGTGGCG AGGCAGGAAA AGAGGACACA
    GTGGAAGAGG AAGAAGGCAA ATTTAACCTC ATGCTCATGG AATGCTCCAT CTGCAATGAA
    ATCATCCACC CTGGATGCCT TAAGATTAAG GAGTCAGAGG GCGTGGTCAA CGATGAGCTT
    CCAAACTGCT GGGAGTGTCC CAAGTGTAAC CACGCCGGCA AGACCGGGAA ACAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195095.1
    CDS16..3927
    Translation

    Target ORF information:

    RefSeq Version XM_005195038.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195038.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGGCAGC TGCTGCGCCC GATTGACCGC CAAAGATACG ACGAGAACGA GGACTTGTCG 
    GACGTGGAGG AGATCGTCAG CGCCCGCGGC TTCAGCCTGG AGGAAAAGCT GCGCAGCCAG
    CTGTACCAGG GGGATTTCGT GCACGCCATG GAGGGCAAAG ATTTCAACTA TGAGTATGTG
    CAGAGAGAAG CTCTCAGAGT TCCCCTGGTA TTTCGAGAAA AGGATGGCCT GGGAATTAAG
    ATGCCTGACC CTGATTTCAC AGTCCGAGAT GTCAAACTCC TAGTGGGTAG CCGACGGCTG
    GTGGACGTGA TGGACGTGAA CACCCAGAAA GGCACGGAGA TGAGCATGTC CCAGTTCGTA
    CGGTACTACG AGACGCCCGA GGCCCAGCGG GACAAGCTGT ACAACGTCAT CAGCCTTGAG
    TTCAGCCACA CCAAGCTGGA GCACTTGGTC AAGCGTCCCA CCGTGGTAGA CCTGGTGGAC
    TGGGTGGACA ACATGTGGCC TCAGCACTTG AAGGAGAAGC AGACGGAAGC CACGAATGCC
    ATTGCAGAGA TGAAGTACCC GAAAGTGAAG AAGTACTGTC TGATGAGTGT GAAGGGTTGT
    TTCACTGACT TCCACATCGA CTTTGGAGGT ACTTCCGTTT GGTACCATGT TTTCCGGGGT
    GGGAAGATTT TTTGGCTGAT TCCTCCAACC TTGCACAATT TGGCACTGTA TGAGGAGTGG
    GTGCTGTCAG GCAAACAGAG CGACATCTTC CTGGGAGACC GCGTGGAGCG ATGCCAAAGA
    ATTGAGCTGA AACAGGGTTA CACGTTTTTC ATCCCTTCCG GCTGGATCCA TGCAGTCTAC
    ACTCCCGTAG ACTCTCTGGT GTTCGGCGGG AACATCCTGC ACAGCTTCAA CGTGCCCATG
    CAGCTGCGGA TCTACGAGAT CGAGGACAGA ACGCGGGTGC AGCCCAAATT CCGTTACCCC
    TTCTACTATG AGATGTGCTG GTATGTCTTG GAGAGATACG TGTACTGTAT GACCCAGCGG
    TCCTACCTCA CTCAGGAATA CCAGAGAGAA TCAATGCTTA TTGATGCCCC AAGAAAGCCC
    AGCATAGACG GCTTCTCATC TGATTCCTGG CTGGAGATGG AGGAGGAGTC CTGTGAGCAG
    CAGCCCCAGG AGGAGAAGGA GGAAGAGGAG GAAGAAGAGG AAGAGGAGGA GGGTGCAGAT
    AAGACCCCCA GAGCATCCAC CGATGGCCCT GCCTCACCCA CGAGCACCCC GTCCGAGGAC
    CAGGAGGCCC CCGGGAAGAA GCCCAAAGCC CCTGCCATGC GGTTCCTCAA AAGGACTCTG
    TCAAACGAGT CAGAGGACAG CGTGAAGTCT GCCACGATGC CCACTGACTA CCCCAAGACG
    CCCACCGGCT CTCCTGCCAC TGAGGTCTCT GCCAAATGGA CCCACCTCAC CGAGTTTGAA
    CTGAAGGGCC TGAAAGCTCT GGTGGAGAAA CTTGAATCCC TCCCGGAGAA CAAGAAGTGT
    GTCCCTGAGG GCATCGAGGA CCCCCAGGCC CTCCTGGAGG GTGTGAAGAA TGTCCTGAAG
    GAGCACGCGG AGGACGACCC CAGTCTGGCC ATCACTGGGG TCCCCGTAGT CACTTGGCCA
    AAGAAGACTC CAAAGAACCG GGCGGTAGGT CGGCCCAAGG GGAAGCTGGG CCCGGCCTCC
    GCGGTGAAGT TGGCCGCCAA CCGGACCACG GCGGGAGCTC GCCGGCGCCG GACGCGATGC
    CGCAAGTGCG AGGCCTGCCT GCGGACGGAG TGCGGCGAGT GCCACTTCTG CAAGGACATG
    AAGAAGTTCG GGGGCCCCGG GCGGATGAAG CAGAGCTGCA TCATGAGGCA GTGCATCGCG
    CCAGTGCTGC CTCACACCGC TGTGTGCCTG GTGTGTGGCG AGGCAGGAAA AGAGGACACA
    GTGGAAGAGG AAGAAGGCAA ATTTAACCTC ATGCTCATGG AATGCTCCAT CTGCAATGAA
    ATCATCCACC CTGGATGCCT TAAGATTAAG GAGTCAGAGG GCGTGGTCAA CGATGAGCTT
    CCAAACTGCT GGGAGTGTCC CAAGTGTAAC CACGCCGGCA AGACCGGGAA ACAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa09014
    Clone ID Related Accession (Same CDS sequence) XM_024977708.1
    Accession Version XM_024977708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAAAAGG AAGCAAGCAG CTTACTCCTC CAGTCCCTTC GCCCGATTGA CCGCCAAAGA 
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833476.1
    CDS16..4053
    Translation

    Target ORF information:

    RefSeq Version XM_024977708.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCAAAAGG AAGCAAGCAG CTTACTCCTC CAGTCCCTTC GCCCGATTGA CCGCCAAAGA 
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224604
    Clone ID Related Accession (Same CDS sequence) XM_024977704.1
    Accession Version XM_024977704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833472.1
    CDS38..4204
    Translation

    Target ORF information:

    RefSeq Version XM_024977704.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224605
    Clone ID Related Accession (Same CDS sequence) XM_024977705.1
    Accession Version XM_024977705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAACAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833473.1
    CDS16..4173
    Translation

    Target ORF information:

    RefSeq Version XM_024977705.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAACAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224606
    Clone ID Related Accession (Same CDS sequence) XM_024977706.1
    Accession Version XM_024977706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833474.1
    CDS16..4089
    Translation

    Target ORF information:

    RefSeq Version XM_024977706.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAAGCCTAC AAGCAAAAGC GTGGCCCTGG CTTTAAGTAC
    GCCTCCAACC TGCCTGGCTC CCTGCTCAAG GAGCAGAAGA TGAACCGGGA CAACAAGGAA
    GGGCAGGAGC CTGCCAAGCG GAGGAGTGAG TGTGAGGAGG CGCCCCGGCG CAGGTCCGAC
    GAGCACCCCA AGAAGGTGCC CCCCGACGGC ATCCTGCGCC GAAAGTCGGA CGACGTGCAC
    CTGAGGCGGA AGCGGAAATA CGAGAAGCCC CAGGAGCTGA GTGGACGCAA GCGAGCCTCG
    ACGCTTCAAA CGTCCCCCGG TTCCTCCTCT CACCTCTCGC CGAGGCCCCC TCTAGGCAGC
    AGCCTCAGCC CCTGGTGGAG ATCCAGTCTC ACTTACTTCC AGCAGCAGCT AAAACCTGGC
    AAAGAAGATA AGCTTTTCAG GAAAAAGCGA CGGTCCTGGA AGAACGCCGA GGACCGGATA
    ACTCTAGCCA ACAAGCCTCT CCGGCGGTTC AAGCAGGAAC CAGAGGACGA CCTACCCGAG
    GCGCCCCCCA AGACCAGGGA GAGCGACCAC TCGCGCTCCA GCTCCCCTAC TGCCGGGCCA
    AGCACAGAGG GCGCGGAGGG GCCCGAGGAG AAGAAGAAGG TGAAGATGCG CCGGAAGCGG
    CGGCTCCCCA ACAAGGAGCT GAGCAAGGAG CTGAGCAAGG AGCTGAACCA GGAGATCCAG
    AAGACGGAGA GCAGCCTGGC CCAGGAGAAT CACCAGCCCA TCAAGTCGGA GCCTGAGAGC
    GAGAACGAGG AGCCCAAGCG GCCCCTGGGC CTCTGCGAGC GCCCCCACCG CTTCAGCAAG
    GGGCTCAACG GCACCCCCCG GGAGCTGCGG CACCAGCTGG GGCCCGGCCT GCGCAGCCCG
    CCCCGAGTCA TCTCCCGGCC CCCTCCCTCC GTGTCCCCGC CCAAGTGCAT CCAGATGGAG
    CGACATGTGA TCCGGCCGCC CCCCATCAGC CCCCCGCCCG ACTCGCTGCC CTTGGATGAC
    GGGGCAGCCC ATGTCATGCA CAGGGAGGTG TGGATGGCCG TCTTCAGCTA CCTCAGCCAC
    CAAGACTTGT GTGTCTGCAT GCGGGTCTGC AAGACCTGGA ACCGCTGGTG CTGCGATAAG
    CGGTTATGGA CCCGCATCGA TTTGAACCAC TGCAAGTCCA TCACGCCCCT GATGCTAAGC
    GGCATCATCC GGCGACAGCC TGTCTCCCTA GACCTCAGCT GGACCAATAT CTCCAAGAAA
    CAGCTGAGCT GGCTCATCAA CCGGTTGCCT GGGCTCCGAG ACTTGGTGCT GTCCGGCTGC
    TCGTGGATCG CAGTCTCTGC CCTCTGTAGC TCCAGCTGTC CACTGCTCCG GACCCTGGAT
    GTCCAGTGGG TAGAAGGGCT GAAGGACGCC CAGATGCGGG ACCTCCTGTC CCCGCCTACA
    GACAACAGGC CAGGTCAGAT GGACAATCGG AGTAAGCTCC GGAACATTGT GGAGCTGCGT
    CTGGCCGGCC TGGACATCAC GGACGCCTCC CTGCGGCTTA TCATCCGCCA CATGCCCCTG
    CTCTCCAAGC TCCACCTCAG TTACTGTAAC CACGTCACTG ACCAGTCCAT CAACCTGCTC
    ACTGCTGTGG GCACCACCAC CCGGGACTCC TTAACCGAAA TCAACCTGTC AGACTGCAAT
    AAGGTGACTG ACCAGTGCCT GTCCTTCTTC AAACGCTGTG GGAATATCTG TCACATTGAC
    CTGAGGTACT GCAAGCAAGT TACCAAGGAA GGCTGCGAGC AGTTCATAGC CGAAATGTCT
    GTTAGTGTCC AGTTCGGGCA AGTGGAAGAA AAACTCCTGC AAAAACTGAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224607
    Clone ID Related Accession (Same CDS sequence) XM_024977707.1
    Accession Version XM_024977707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAACAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833475.1
    CDS16..4080
    Translation

    Target ORF information:

    RefSeq Version XM_024977707.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCCGCCGG CCCCCGGTTC CGGCTTCCCG GCTCTGCCGC CGCGGCCGCC AGGGCTGCTG 
    TTACCGCCGA GCCGGGCGCT GCGGCGGCTC CTCCCGGGGC CTACAAATGC CCCGCCAGCC
    CGTGTGCGGA GCCCCCCTCG CGGGTTACAT AACCAGGCTC CCGGTCGACG CCCGATTGAC
    CGCCAAAGAT ACGACGAGAA CGAGGACTTG TCGGACGTGG AGGAGATCGT CAGCGCCCGC
    GGCTTCAGCC TGGAGGAAAA GCTGCGCAGC CAGCTGTACC AGGGGGATTT CGTGCACGCC
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGCCAGTGC TGCCTCACAC CGCTGTGTGC
    CTGGTGTGTG GCGAGGCAGG AAAAGAGGAC ACAGTGGAAG AGGAAGAAGG CAAATTTAAC
    CTCATGCTCA TGGAATGCTC CATCTGCAAT GAAATCATCC ACCCTGGATG CCTTAAGATT
    AAGGAGTCAG AGGGCGTGGT CAACGATGAG CTTCCAAACT GCTGGGAGTG TCCCAAGTGT
    AACCACGCCG GCAAGACCGG GAAACAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224608
    Clone ID Related Accession (Same CDS sequence) XM_010814030.3 , XM_024977709.1
    Accession Version XM_010814030.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG 
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812332.2
    CDS657..4523
    Translation

    Target ORF information:

    RefSeq Version XM_010814030.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814030.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG 
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224608
    Clone ID Related Accession (Same CDS sequence) XM_010814030.3 , XM_024977709.1
    Accession Version XM_024977709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG 
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833477.1
    CDS167..4033
    Translation

    Target ORF information:

    RefSeq Version XM_024977709.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGGGCA AAGATTTCAA CTATGAGTAT GTGCAGAGAG AAGCTCTCAG AGTTCCCCTG 
    GTATTTCGAG AAAAGGATGG CCTGGGAATT AAGATGCCTG ACCCTGATTT CACAGTCCGA
    GATGTCAAAC TCCTAGTGGG TAGCCGACGG CTGGTGGACG TGATGGACGT GAACACCCAG
    AAAGGCACGG AGATGAGCAT GTCCCAGTTC GTACGGTACT ACGAGACGCC CGAGGCCCAG
    CGGGACAAGC TGTACAACGT CATCAGCCTT GAGTTCAGCC ACACCAAGCT GGAGCACTTG
    GTCAAGCGTC CCACCGTGGT AGACCTGGTG GACTGGGTGG ACAACATGTG GCCTCAGCAC
    TTGAAGGAGA AGCAGACGGA AGCCACGAAT GCCATTGCAG AGATGAAGTA CCCGAAAGTG
    AAGAAGTACT GTCTGATGAG TGTGAAGGGT TGTTTCACTG ACTTCCACAT CGACTTTGGA
    GGTACTTCCG TTTGGTACCA TGTTTTCCGG GGTGGGAAGA TTTTTTGGCT GATTCCTCCA
    ACCTTGCACA ATTTGGCACT GTATGAGGAG TGGGTGCTGT CAGGCAAACA GAGCGACATC
    TTCCTGGGAG ACCGCGTGGA GCGATGCCAA AGAATTGAGC TGAAACAGGG TTACACGTTT
    TTCATCCCTT CCGGCTGGAT CCATGCAGTC TACACTCCCG TAGACTCTCT GGTGTTCGGC
    GGGAACATCC TGCACAGCTT CAACGTGCCC ATGCAGCTGC GGATCTACGA GATCGAGGAC
    AGAACGCGGG TGCAGCCCAA ATTCCGTTAC CCCTTCTACT ATGAGATGTG CTGGTATGTC
    TTGGAGAGAT ACGTGTACTG TATGACCCAG CGGTCCTACC TCACTCAGGA ATACCAGAGA
    GAATCAATGC TTATTGATGC CCCAAGAAAG CCCAGCATAG ACGGCTTCTC ATCTGATTCC
    TGGCTGGAGA TGGAGGAGGA GTCCTGTGAG CAGCAGCCCC AGGAGGAGAA GGAGGAAGAG
    GAGGAAGAAG AGGAAGAGGA GGAGGGTGCA GATAAGACCC CCAGAGCATC CACCGATGGC
    CCTGCCTCAC CCACGAGCAC CCCGTCCGAG GACCAGGAGG CCCCCGGGAA GAAGCCCAAA
    GCCCCTGCCA TGCGGTTCCT CAAAAGGACT CTGTCAAACG AGTCAGAGGA CAGCGTGAAG
    TCTGCCACGA TGCCCACTGA CTACCCCAAG ACGCCCACCG GCTCTCCTGC CACTGAGGTC
    TCTGCCAAAT GGACCCACCT CACCGAGTTT GAACTGAAGG GCCTGAAAGC TCTGGTGGAG
    AAACTTGAAT CCCTCCCGGA GAACAAGAAG TGTGTCCCTG AGGGCATCGA GGACCCCCAG
    GCCCTCCTGG AGGGTGTGAA GAATGTCCTG AAGGAGCACG CGGAGGACGA CCCCAGTCTG
    GCCATCACTG GGGTCCCCGT AGTCACTTGG CCAAAGAAGA CTCCAAAGAA CCGGGCGGTA
    GGTCGGCCCA AGGGGAAGCT GGGCCCGGCC TCCGCGGTGA AGTTGGCCGC CAACCGGACC
    ACGGCGGGAG CTCGCCGGCG CCGGACGCGA TGCCGCAAGT GCGAGGCCTG CCTGCGGACG
    GAGTGCGGCG AGTGCCACTT CTGCAAGGAC ATGAAGAAGT TCGGGGGCCC CGGGCGGATG
    AAGCAGAGCT GCATCATGAG GCAGTGCATC GCGGGCACTG TGCCTCCAGC CCCAGGGAGG
    GAGTGGCGAT CTCCCTGCTC CCCGGGGACA GCAGGCTCAG CGGGAGGATC AAGGCCTCCG
    GCCCAGCCAG TGCTGCCTCA CACCGCTGTG TGCCTGGTGT GTGGCGAGGC AGGAAAAGAG
    GACACAGTGG AAGAGGAAGA AGGCAAATTT AACCTCATGC TCATGGAATG CTCCATCTGC
    AATGAAATCA TCCACCCTGG ATGCCTTAAG ATTAAGGAGT CAGAGGGCGT GGTCAACGAT
    GAGCTTCCAA ACTGCTGGGA GTGTCCCAAG TGTAACCACG CCGGCAAGAC CGGGAAAGCC
    TACAAGCAAA AGCGTGGCCC TGGCTTTAAG TACGCCTCCA ACCTGCCTGG CTCCCTGCTC
    AAGGAGCAGA AGATGAACCG GGACAACAAG GAAGGGCAGG AGCCTGCCAA GCGGAGGAGT
    GAGTGTGAGG AGGCGCCCCG GCGCAGGTCC GACGAGCACC CCAAGAAGGT GCCCCCCGAC
    GGCATCCTGC GCCGAAAGTC GGACGACGTG CACCTGAGGC GGAAGCGGAA ATACGAGAAG
    CCCCAGGAGC TGAGTGGACG CAAGCGAGCC TCGACGCTTC AAACGTCCCC CGGTTCCTCC
    TCTCACCTCT CGCCGAGGCC CCCTCTAGGC AGCAGCCTCA GCCCCTGGTG GAGATCCAGT
    CTCACTTACT TCCAGCAGCA GCTAAAACCT GGCAAAGAAG ATAAGCTTTT CAGGAAAAAG
    CGACGGTCCT GGAAGAACGC CGAGGACCGG ATAACTCTAG CCAACAAGCC TCTCCGGCGG
    TTCAAGCAGG AACCAGAGGA CGACCTACCC GAGGCGCCCC CCAAGACCAG GGAGAGCGAC
    CACTCGCGCT CCAGCTCCCC TACTGCCGGG CCAAGCACAG AGGGCGCGGA GGGGCCCGAG
    GAGAAGAAGA AGGTGAAGAT GCGCCGGAAG CGGCGGCTCC CCAACAAGGA GCTGAGCAAG
    GAGCTGAGCA AGGAGCTGAA CCAGGAGATC CAGAAGACGG AGAGCAGCCT GGCCCAGGAG
    AATCACCAGC CCATCAAGTC GGAGCCTGAG AGCGAGAACG AGGAGCCCAA GCGGCCCCTG
    GGCCTCTGCG AGCGCCCCCA CCGCTTCAGC AAGGGGCTCA ACGGCACCCC CCGGGAGCTG
    CGGCACCAGC TGGGGCCCGG CCTGCGCAGC CCGCCCCGAG TCATCTCCCG GCCCCCTCCC
    TCCGTGTCCC CGCCCAAGTG CATCCAGATG GAGCGACATG TGATCCGGCC GCCCCCCATC
    AGCCCCCCGC CCGACTCGCT GCCCTTGGAT GACGGGGCAG CCCATGTCAT GCACAGGGAG
    GTGTGGATGG CCGTCTTCAG CTACCTCAGC CACCAAGACT TGTGTGTCTG CATGCGGGTC
    TGCAAGACCT GGAACCGCTG GTGCTGCGAT AAGCGGTTAT GGACCCGCAT CGATTTGAAC
    CACTGCAAGT CCATCACGCC CCTGATGCTA AGCGGCATCA TCCGGCGACA GCCTGTCTCC
    CTAGACCTCA GCTGGACCAA TATCTCCAAG AAACAGCTGA GCTGGCTCAT CAACCGGTTG
    CCTGGGCTCC GAGACTTGGT GCTGTCCGGC TGCTCGTGGA TCGCAGTCTC TGCCCTCTGT
    AGCTCCAGCT GTCCACTGCT CCGGACCCTG GATGTCCAGT GGGTAGAAGG GCTGAAGGAC
    GCCCAGATGC GGGACCTCCT GTCCCCGCCT ACAGACAACA GGCCAGGTCA GATGGACAAT
    CGGAGTAAGC TCCGGAACAT TGTGGAGCTG CGTCTGGCCG GCCTGGACAT CACGGACGCC
    TCCCTGCGGC TTATCATCCG CCACATGCCC CTGCTCTCCA AGCTCCACCT CAGTTACTGT
    AACCACGTCA CTGACCAGTC CATCAACCTG CTCACTGCTG TGGGCACCAC CACCCGGGAC
    TCCTTAACCG AAATCAACCT GTCAGACTGC AATAAGGTGA CTGACCAGTG CCTGTCCTTC
    TTCAAACGCT GTGGGAATAT CTGTCACATT GACCTGAGGT ACTGCAAGCA AGTTACCAAG
    GAAGGCTGCG AGCAGTTCAT AGCCGAAATG TCTGTTAGTG TCCAGTTCGG GCAAGTGGAA
    GAAAAACTCC TGCAAAAACT GAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224609
    Clone ID Related Accession (Same CDS sequence) XM_024977710.1
    Accession Version XM_024977710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG 
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833478.1
    CDS140..3571
    Translation

    Target ORF information:

    RefSeq Version XM_024977710.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG 
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGGG CACTGTGCCT CCAGCCCCAG GGAGGGAGTG GCGATCTCCC
    TGCTCCCCGG GGACAGCAGG CTCAGCGGGA GGATCAAGGC CTCCGGCCCA GCCAGTGCTG
    CCTCACACCG CTGTGTGCCT GGTGTGTGGC GAGGCAGGAA AAGAGGACAC AGTGGAAGAG
    GAAGAAGGCA AATTTAACCT CATGCTCATG GAATGCTCCA TCTGCAATGA AATCATCCAC
    CCTGGATGCC TTAAGATTAA GGAGTCAGAG GGCGTGGTCA ACGATGAGCT TCCAAACTGC
    TGGGAGTGTC CCAAGTGTAA CCACGCCGGC AAGACCGGGA AAGCCTACAA GCAAAAGCGT
    GGCCCTGGCT TTAAGTACGC CTCCAACCTG CCTGGCTCCC TGCTCAAGGA GCAGAAGATG
    AACCGGGACA ACAAGGAAGG GCAGGAGCCT GCCAAGCGGA GGAGTGAGTG TGAGGAGGCG
    CCCCGGCGCA GGTCCGACGA GCACCCCAAG AAGGTGCCCC CCGACGGCAT CCTGCGCCGA
    AAGTCGGACG ACGTGCACCT GAGGCGGAAG CGGAAATACG AGAAGCCCCA GGAGCTGAGT
    GGACGCAAGC GAGCCTCGAC GCTTCAAACG TCCCCCGGTT CCTCCTCTCA CCTCTCGCCG
    AGGCCCCCTC TAGGCAGCAG CCTCAGCCCC TGGTGGAGAT CCAGTCTCAC TTACTTCCAG
    CAGCAGCTAA AACCTGGCAA AGAAGATAAG CTTTTCAGGA AAAAGCGACG GTCCTGGAAG
    AACGCCGAGG ACCGGATAAC TCTAGCCAAC AAGCCTCTCC GGCGGTTCAA GCAGGAACCA
    GAGGACGACC TACCCGAGGC GCCCCCCAAG ACCAGGGAGA GCGACCACTC GCGCTCCAGC
    TCCCCTACTG CCGGGCCAAG CACAGAGGGC GCGGAGGGGC CCGAGGAGAA GAAGAAGGTG
    AAGATGCGCC GGAAGCGGCG GCTCCCCAAC AAGGAGCTGA GCAAGGAGCT GAGCAAGGAG
    CTGAACCAGG AGATCCAGAA GACGGAGAGC AGCCTGGCCC AGGAGAATCA CCAGCCCATC
    AAGTCGGAGC CTGAGAGCGA GAACGAGGAG CCCAAGCGGC CCCTGGGCCT CTGCGAGCGC
    CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG AGCTGCGGCA CCAGCTGGGG
    CCCGGCCTGC GCAGCCCGCC CCGAGTCATC TCCCGGCCCC CTCCCTCCGT GTCCCCGCCC
    AAGTGCATCC AGATGGAGCG ACATGTGATC CGGCCGCCCC CCATCAGCCC CCCGCCCGAC
    TCGCTGCCCT TGGATGACGG GGCAGCCCAT GTCATGCACA GGGAGGTGTG GATGGCCGTC
    TTCAGCTACC TCAGCCACCA AGACTTGTGT GTCTGCATGC GGGTCTGCAA GACCTGGAAC
    CGCTGGTGCT GCGATAAGCG GTTATGGACC CGCATCGATT TGAACCACTG CAAGTCCATC
    ACGCCCCTGA TGCTAAGCGG CATCATCCGG CGACAGCCTG TCTCCCTAGA CCTCAGCTGG
    ACCAATATCT CCAAGAAACA GCTGAGCTGG CTCATCAACC GGTTGCCTGG GCTCCGAGAC
    TTGGTGCTGT CCGGCTGCTC GTGGATCGCA GTCTCTGCCC TCTGTAGCTC CAGCTGTCCA
    CTGCTCCGGA CCCTGGATGT CCAGTGGGTA GAAGGGCTGA AGGACGCCCA GATGCGGGAC
    CTCCTGTCCC CGCCTACAGA CAACAGGCCA GGTCAGATGG ACAATCGGAG TAAGCTCCGG
    AACATTGTGG AGCTGCGTCT GGCCGGCCTG GACATCACGG ACGCCTCCCT GCGGCTTATC
    ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT ACTGTAACCA CGTCACTGAC
    CAGTCCATCA ACCTGCTCAC TGCTGTGGGC ACCACCACCC GGGACTCCTT AACCGAAATC
    AACCTGTCAG ACTGCAATAA GGTGACTGAC CAGTGCCTGT CCTTCTTCAA ACGCTGTGGG
    AATATCTGTC ACATTGACCT GAGGTACTGC AAGCAAGTTA CCAAGGAAGG CTGCGAGCAG
    TTCATAGCCG AAATGTCTGT TAGTGTCCAG TTCGGGCAAG TGGAAGAAAA ACTCCTGCAA
    AAACTGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224610
    Clone ID Related Accession (Same CDS sequence) XM_024977711.1
    Accession Version XM_024977711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG 
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833479.1
    CDS294..3623
    Translation

    Target ORF information:

    RefSeq Version XM_024977711.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2B (KDM2B), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAGTGTGA AGGGTTGTTT CACTGACTTC CACATCGACT TTGGAGGTAC TTCCGTTTGG 
    TACCATGTTT TCCGGGGTGG GAAGATTTTT TGGCTGATTC CTCCAACCTT GCACAATTTG
    GCACTGTATG AGGAGTGGGT GCTGTCAGGC AAACAGAGCG ACATCTTCCT GGGAGACCGC
    GTGGAGCGAT GCCAAAGAAT TGAGCTGAAA CAGGGTTACA CGTTTTTCAT CCCTTCCGGC
    TGGATCCATG CAGTCTACAC TCCCGTAGAC TCTCTGGTGT TCGGCGGGAA CATCCTGCAC
    AGCTTCAACG TGCCCATGCA GCTGCGGATC TACGAGATCG AGGACAGAAC GCGGGTGCAG
    CCCAAATTCC GTTACCCCTT CTACTATGAG ATGTGCTGGT ATGTCTTGGA GAGATACGTG
    TACTGTATGA CCCAGCGGTC CTACCTCACT CAGGAATACC AGAGAGAATC AATGCTTATT
    GATGCCCCAA GAAAGCCCAG CATAGACGGC TTCTCATCTG ATTCCTGGCT GGAGATGGAG
    GAGGAGTCCT GTGAGCAGCA GCCCCAGGAG GAGAAGGAGG AAGAGGAGGA AGAAGAGGAA
    GAGGAGGAGG GTGCAGATAA GACCCCCAGA GCATCCACCG ATGGCCCTGC CTCACCCACG
    AGCACCCCGT CCGAGGACCA GGAGGCCCCC GGGAAGAAGC CCAAAGCCCC TGCCATGCGG
    TTCCTCAAAA GGACTCTGTC AAACGAGTCA GAGGACAGCG TGAAGTCTGC CACGATGCCC
    ACTGACTACC CCAAGACGCC CACCGGCTCT CCTGCCACTG AGGTCTCTGC CAAATGGACC
    CACCTCACCG AGTTTGAACT GAAGGGCCTG AAAGCTCTGG TGGAGAAACT TGAATCCCTC
    CCGGAGAACA AGAAGTGTGT CCCTGAGGGC ATCGAGGACC CCCAGGCCCT CCTGGAGGGT
    GTGAAGAATG TCCTGAAGGA GCACGCGGAG GACGACCCCA GTCTGGCCAT CACTGGGGTC
    CCCGTAGTCA CTTGGCCAAA GAAGACTCCA AAGAACCGGG CGGTAGGTCG GCCCAAGGGG
    AAGCTGGGCC CGGCCTCCGC GGTGAAGTTG GCCGCCAACC GGACCACGGC GGGAGCTCGC
    CGGCGCCGGA CGCGATGCCG CAAGTGCGAG GCCTGCCTGC GGACGGAGTG CGGCGAGTGC
    CACTTCTGCA AGGACATGAA GAAGTTCGGG GGCCCCGGGC GGATGAAGCA GAGCTGCATC
    ATGAGGCAGT GCATCGCGCC AGTGCTGCCT CACACCGCTG TGTGCCTGGT GTGTGGCGAG
    GCAGGAAAAG AGGACACAGT GGAAGAGGAA GAAGGCAAAT TTAACCTCAT GCTCATGGAA
    TGCTCCATCT GCAATGAAAT CATCCACCCT GGATGCCTTA AGATTAAGGA GTCAGAGGGC
    GTGGTCAACG ATGAGCTTCC AAACTGCTGG GAGTGTCCCA AGTGTAACCA CGCCGGCAAG
    ACCGGGAAAC AAAAGCGTGG CCCTGGCTTT AAGTACGCCT CCAACCTGCC TGGCTCCCTG
    CTCAAGGAGC AGAAGATGAA CCGGGACAAC AAGGAAGGGC AGGAGCCTGC CAAGCGGAGG
    AGTGAGTGTG AGGAGGCGCC CCGGCGCAGG TCCGACGAGC ACCCCAAGAA GGTGCCCCCC
    GACGGCATCC TGCGCCGAAA GTCGGACGAC GTGCACCTGA GGCGGAAGCG GAAATACGAG
    AAGCCCCAGG AGCTGAGTGG ACGCAAGCGA GCCTCGACGC TTCAAACGTC CCCCGGTTCC
    TCCTCTCACC TCTCGCCGAG GCCCCCTCTA GGCAGCAGCC TCAGCCCCTG GTGGAGATCC
    AGTCTCACTT ACTTCCAGCA GCAGCTAAAA CCTGGCAAAG AAGATAAGCT TTTCAGGAAA
    AAGCGACGGT CCTGGAAGAA CGCCGAGGAC CGGATAACTC TAGCCAACAA GCCTCTCCGG
    CGGTTCAAGC AGGAACCAGA GGACGACCTA CCCGAGGCGC CCCCCAAGAC CAGGGAGAGC
    GACCACTCGC GCTCCAGCTC CCCTACTGCC GGGCCAAGCA CAGAGGGCGC GGAGGGGCCC
    GAGGAGAAGA AGAAGGTGAA GATGCGCCGG AAGCGGCGGC TCCCCAACAA GGAGCTGAGC
    AAGGAGCTGA GCAAGGAGCT GAACCAGGAG ATCCAGAAGA CGGAGAGCAG CCTGGCCCAG
    GAGAATCACC AGCCCATCAA GTCGGAGCCT GAGAGCGAGA ACGAGGAGCC CAAGCGGCCC
    CTGGGCCTCT GCGAGCGCCC CCACCGCTTC AGCAAGGGGC TCAACGGCAC CCCCCGGGAG
    CTGCGGCACC AGCTGGGGCC CGGCCTGCGC AGCCCGCCCC GAGTCATCTC CCGGCCCCCT
    CCCTCCGTGT CCCCGCCCAA GTGCATCCAG ATGGAGCGAC ATGTGATCCG GCCGCCCCCC
    ATCAGCCCCC CGCCCGACTC GCTGCCCTTG GATGACGGGG CAGCCCATGT CATGCACAGG
    GAGGTGTGGA TGGCCGTCTT CAGCTACCTC AGCCACCAAG ACTTGTGTGT CTGCATGCGG
    GTCTGCAAGA CCTGGAACCG CTGGTGCTGC GATAAGCGGT TATGGACCCG CATCGATTTG
    AACCACTGCA AGTCCATCAC GCCCCTGATG CTAAGCGGCA TCATCCGGCG ACAGCCTGTC
    TCCCTAGACC TCAGCTGGAC CAATATCTCC AAGAAACAGC TGAGCTGGCT CATCAACCGG
    TTGCCTGGGC TCCGAGACTT GGTGCTGTCC GGCTGCTCGT GGATCGCAGT CTCTGCCCTC
    TGTAGCTCCA GCTGTCCACT GCTCCGGACC CTGGATGTCC AGTGGGTAGA AGGGCTGAAG
    GACGCCCAGA TGCGGGACCT CCTGTCCCCG CCTACAGACA ACAGGCCAGG TCAGATGGAC
    AATCGGAGTA AGCTCCGGAA CATTGTGGAG CTGCGTCTGG CCGGCCTGGA CATCACGGAC
    GCCTCCCTGC GGCTTATCAT CCGCCACATG CCCCTGCTCT CCAAGCTCCA CCTCAGTTAC
    TGTAACCACG TCACTGACCA GTCCATCAAC CTGCTCACTG CTGTGGGCAC CACCACCCGG
    GACTCCTTAA CCGAAATCAA CCTGTCAGAC TGCAATAAGG TGACTGACCA GTGCCTGTCC
    TTCTTCAAAC GCTGTGGGAA TATCTGTCAC ATTGACCTGA GGTACTGCAA GCAAGTTACC
    AAGGAAGGCT GCGAGCAGTT CATAGCCGAA ATGTCTGTTA GTGTCCAGTT CGGGCAAGTG
    GAAGAAAAAC TCCTGCAAAA ACTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55412
    Clone ID Related Accession (Same CDS sequence) XM_010823384.2 , XM_010814030.2
    Accession Version XM_010823384.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821686.1
    CDS210..4727
    Translation

    Target ORF information:

    RefSeq Version XM_010823384.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55412
    Clone ID Related Accession (Same CDS sequence) XM_010823384.2 , XM_010814030.2
    Accession Version XM_010814030.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812332.1
    CDS210..4727
    Translation

    Target ORF information:

    RefSeq Version XM_010814030.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAAGC CTACAAGCAA
    AAGCGTGGCC CTGGCTTTAA GTACGCCTCC AACCTGCCTG GCTCCCTGCT CAAGGAGCAG
    AAGATGAACC GGGACAACAA GGAAGGGCAG GAGCCTGCCA AGCGGAGGAG TGAGTGTGAG
    GAGGCGCCCC GGCGCAGGTC CGACGAGCAC CCCAAGAAGG TGCCCCCCGA CGGCATCCTG
    CGCCGAAAGT CGGACGACGT GCACCTGAGG CGGAAGCGGA AATACGAGAA GCCCCAGGAG
    CTGAGTGGAC GCAAGCGAGC CTCGACGCTT CAAACGTCCC CCGGTTCCTC CTCTCACCTC
    TCGCCGAGGC CCCCTCTAGG CAGCAGCCTC AGCCCCTGGT GGAGATCCAG TCTCACTTAC
    TTCCAGCAGC AGCTAAAACC TGGCAAAGAA GATAAGCTTT TCAGGAAAAA GCGACGGTCC
    TGGAAGAACG CCGAGGACCG GATAACTCTA GCCAACAAGC CTCTCCGGCG GTTCAAGCAG
    GAACCAGAGG ACGACCTACC CGAGGCGCCC CCCAAGACCA GGGAGAGCGA CCACTCGCGC
    TCCAGCTCCC CTACTGCCGG GCCAAGCACA GAGGGCGCGG AGGGGCCCGA GGAGAAGAAG
    AAGGTGAAGA TGCGCCGGAA GCGGCGGCTC CCCAACAAGG AGCTGAGCAA GGAGCTGAGC
    AAGGAGCTGA ACCAGGAGAT CCAGAAGACG GAGAGCAGCC TGGCCCAGGA GAATCACCAG
    CCCATCAAGT CGGAGCCTGA GAGCGAGAAC GAGGAGCCCA AGCGGCCCCT GGGCCTCTGC
    GAGCGCCCCC ACCGCTTCAG CAAGGGGCTC AACGGCACCC CCCGGGAGCT GCGGCACCAG
    CTGGGGCCCG GCCTGCGCAG CCCGCCCCGA GTCATCTCCC GGCCCCCTCC CTCCGTGTCC
    CCGCCCAAGT GCATCCAGAT GGAGCGACAT GTGATCCGGC CGCCCCCCAT CAGCCCCCCG
    CCCGACTCGC TGCCCTTGGA TGACGGGGCA GCCCATGTCA TGCACAGGGA GGTGTGGATG
    GCCGTCTTCA GCTACCTCAG CCACCAAGAC TTGTGTGTCT GCATGCGGGT CTGCAAGACC
    TGGAACCGCT GGTGCTGCGA TAAGCGGTTA TGGACCCGCA TCGATTTGAA CCACTGCAAG
    TCCATCACGC CCCTGATGCT AAGCGGCATC ATCCGGCGAC AGCCTGTCTC CCTAGACCTC
    AGCTGGACCA ATATCTCCAA GAAACAGCTG AGCTGGCTCA TCAACCGGTT GCCTGGGCTC
    CGAGACTTGG TGCTGTCCGG CTGCTCGTGG ATCGCAGTCT CTGCCCTCTG TAGCTCCAGC
    TGTCCACTGC TCCGGACCCT GGATGTCCAG TGGGTAGAAG GGCTGAAGGA CGCCCAGATG
    CGGGACCTCC TGTCCCCGCC TACAGACAAC AGGCCAGGTC AGATGGACAA TCGGAGTAAG
    CTCCGGAACA TTGTGGAGCT GCGTCTGGCC GGCCTGGACA TCACGGACGC CTCCCTGCGG
    CTTATCATCC GCCACATGCC CCTGCTCTCC AAGCTCCACC TCAGTTACTG TAACCACGTC
    ACTGACCAGT CCATCAACCT GCTCACTGCT GTGGGCACCA CCACCCGGGA CTCCTTAACC
    GAAATCAACC TGTCAGACTG CAATAAGGTG ACTGACCAGT GCCTGTCCTT CTTCAAACGC
    TGTGGGAATA TCTGTCACAT TGACCTGAGG TACTGCAAGC AAGTTACCAA GGAAGGCTGC
    GAGCAGTTCA TAGCCGAAAT GTCTGTTAGT GTCCAGTTCG GGCAAGTGGA AGAAAAACTC
    CTGCAAAAAC TGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55413
    Clone ID Related Accession (Same CDS sequence) XM_010823385.2 , XM_010814031.2
    Accession Version XM_010823385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAACA AAAGCGTGGC
    CCTGGCTTTA AGTACGCCTC CAACCTGCCT GGCTCCCTGC TCAAGGAGCA GAAGATGAAC
    CGGGACAACA AGGAAGGGCA GGAGCCTGCC AAGCGGAGGA GTGAGTGTGA GGAGGCGCCC
    CGGCGCAGGT CCGACGAGCA CCCCAAGAAG GTGCCCCCCG ACGGCATCCT GCGCCGAAAG
    TCGGACGACG TGCACCTGAG GCGGAAGCGG AAATACGAGA AGCCCCAGGA GCTGAGTGGA
    CGCAAGCGAG CCTCGACGCT TCAAACGTCC CCCGGTTCCT CCTCTCACCT CTCGCCGAGG
    CCCCCTCTAG GCAGCAGCCT CAGCCCCTGG TGGAGATCCA GTCTCACTTA CTTCCAGCAG
    CAGCTAAAAC CTGGCAAAGA AGATAAGCTT TTCAGGAAAA AGCGACGGTC CTGGAAGAAC
    GCCGAGGACC GGATAACTCT AGCCAACAAG CCTCTCCGGC GGTTCAAGCA GGAACCAGAG
    GACGACCTAC CCGAGGCGCC CCCCAAGACC AGGGAGAGCG ACCACTCGCG CTCCAGCTCC
    CCTACTGCCG GGCCAAGCAC AGAGGGCGCG GAGGGGCCCG AGGAGAAGAA GAAGGTGAAG
    ATGCGCCGGA AGCGGCGGCT CCCCAACAAG GAGCTGAGCA AGGAGCTGAG CAAGGAGCTG
    AACCAGGAGA TCCAGAAGAC GGAGAGCAGC CTGGCCCAGG AGAATCACCA GCCCATCAAG
    TCGGAGCCTG AGAGCGAGAA CGAGGAGCCC AAGCGGCCCC TGGGCCTCTG CGAGCGCCCC
    CACCGCTTCA GCAAGGGGCT CAACGGCACC CCCCGGGAGC TGCGGCACCA GCTGGGGCCC
    GGCCTGCGCA GCCCGCCCCG AGTCATCTCC CGGCCCCCTC CCTCCGTGTC CCCGCCCAAG
    TGCATCCAGA TGGAGCGACA TGTGATCCGG CCGCCCCCCA TCAGCCCCCC GCCCGACTCG
    CTGCCCTTGG ATGACGGGGC AGCCCATGTC ATGCACAGGG AGGTGTGGAT GGCCGTCTTC
    AGCTACCTCA GCCACCAAGA CTTGTGTGTC TGCATGCGGG TCTGCAAGAC CTGGAACCGC
    TGGTGCTGCG ATAAGCGGTT ATGGACCCGC ATCGATTTGA ACCACTGCAA GTCCATCACG
    CCCCTGATGC TAAGCGGCAT CATCCGGCGA CAGCCTGTCT CCCTAGACCT CAGCTGGACC
    AATATCTCCA AGAAACAGCT GAGCTGGCTC ATCAACCGGT TGCCTGGGCT CCGAGACTTG
    GTGCTGTCCG GCTGCTCGTG GATCGCAGTC TCTGCCCTCT GTAGCTCCAG CTGTCCACTG
    CTCCGGACCC TGGATGTCCA GTGGGTAGAA GGGCTGAAGG ACGCCCAGAT GCGGGACCTC
    CTGTCCCCGC CTACAGACAA CAGGCCAGGT CAGATGGACA ATCGGAGTAA GCTCCGGAAC
    ATTGTGGAGC TGCGTCTGGC CGGCCTGGAC ATCACGGACG CCTCCCTGCG GCTTATCATC
    CGCCACATGC CCCTGCTCTC CAAGCTCCAC CTCAGTTACT GTAACCACGT CACTGACCAG
    TCCATCAACC TGCTCACTGC TGTGGGCACC ACCACCCGGG ACTCCTTAAC CGAAATCAAC
    CTGTCAGACT GCAATAAGGT GACTGACCAG TGCCTGTCCT TCTTCAAACG CTGTGGGAAT
    ATCTGTCACA TTGACCTGAG GTACTGCAAG CAAGTTACCA AGGAAGGCTG CGAGCAGTTC
    ATAGCCGAAA TGTCTGTTAG TGTCCAGTTC GGGCAAGTGG AAGAAAAACT CCTGCAAAAA
    CTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821687.1
    CDS209..4717
    Translation

    Target ORF information:

    RefSeq Version XM_010823385.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAACA AAAGCGTGGC
    CCTGGCTTTA AGTACGCCTC CAACCTGCCT GGCTCCCTGC TCAAGGAGCA GAAGATGAAC
    CGGGACAACA AGGAAGGGCA GGAGCCTGCC AAGCGGAGGA GTGAGTGTGA GGAGGCGCCC
    CGGCGCAGGT CCGACGAGCA CCCCAAGAAG GTGCCCCCCG ACGGCATCCT GCGCCGAAAG
    TCGGACGACG TGCACCTGAG GCGGAAGCGG AAATACGAGA AGCCCCAGGA GCTGAGTGGA
    CGCAAGCGAG CCTCGACGCT TCAAACGTCC CCCGGTTCCT CCTCTCACCT CTCGCCGAGG
    CCCCCTCTAG GCAGCAGCCT CAGCCCCTGG TGGAGATCCA GTCTCACTTA CTTCCAGCAG
    CAGCTAAAAC CTGGCAAAGA AGATAAGCTT TTCAGGAAAA AGCGACGGTC CTGGAAGAAC
    GCCGAGGACC GGATAACTCT AGCCAACAAG CCTCTCCGGC GGTTCAAGCA GGAACCAGAG
    GACGACCTAC CCGAGGCGCC CCCCAAGACC AGGGAGAGCG ACCACTCGCG CTCCAGCTCC
    CCTACTGCCG GGCCAAGCAC AGAGGGCGCG GAGGGGCCCG AGGAGAAGAA GAAGGTGAAG
    ATGCGCCGGA AGCGGCGGCT CCCCAACAAG GAGCTGAGCA AGGAGCTGAG CAAGGAGCTG
    AACCAGGAGA TCCAGAAGAC GGAGAGCAGC CTGGCCCAGG AGAATCACCA GCCCATCAAG
    TCGGAGCCTG AGAGCGAGAA CGAGGAGCCC AAGCGGCCCC TGGGCCTCTG CGAGCGCCCC
    CACCGCTTCA GCAAGGGGCT CAACGGCACC CCCCGGGAGC TGCGGCACCA GCTGGGGCCC
    GGCCTGCGCA GCCCGCCCCG AGTCATCTCC CGGCCCCCTC CCTCCGTGTC CCCGCCCAAG
    TGCATCCAGA TGGAGCGACA TGTGATCCGG CCGCCCCCCA TCAGCCCCCC GCCCGACTCG
    CTGCCCTTGG ATGACGGGGC AGCCCATGTC ATGCACAGGG AGGTGTGGAT GGCCGTCTTC
    AGCTACCTCA GCCACCAAGA CTTGTGTGTC TGCATGCGGG TCTGCAAGAC CTGGAACCGC
    TGGTGCTGCG ATAAGCGGTT ATGGACCCGC ATCGATTTGA ACCACTGCAA GTCCATCACG
    CCCCTGATGC TAAGCGGCAT CATCCGGCGA CAGCCTGTCT CCCTAGACCT CAGCTGGACC
    AATATCTCCA AGAAACAGCT GAGCTGGCTC ATCAACCGGT TGCCTGGGCT CCGAGACTTG
    GTGCTGTCCG GCTGCTCGTG GATCGCAGTC TCTGCCCTCT GTAGCTCCAG CTGTCCACTG
    CTCCGGACCC TGGATGTCCA GTGGGTAGAA GGGCTGAAGG ACGCCCAGAT GCGGGACCTC
    CTGTCCCCGC CTACAGACAA CAGGCCAGGT CAGATGGACA ATCGGAGTAA GCTCCGGAAC
    ATTGTGGAGC TGCGTCTGGC CGGCCTGGAC ATCACGGACG CCTCCCTGCG GCTTATCATC
    CGCCACATGC CCCTGCTCTC CAAGCTCCAC CTCAGTTACT GTAACCACGT CACTGACCAG
    TCCATCAACC TGCTCACTGC TGTGGGCACC ACCACCCGGG ACTCCTTAAC CGAAATCAAC
    CTGTCAGACT GCAATAAGGT GACTGACCAG TGCCTGTCCT TCTTCAAACG CTGTGGGAAT
    ATCTGTCACA TTGACCTGAG GTACTGCAAG CAAGTTACCA AGGAAGGCTG CGAGCAGTTC
    ATAGCCGAAA TGTCTGTTAG TGTCCAGTTC GGGCAAGTGG AAGAAAAACT CCTGCAAAAA
    CTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55413
    Clone ID Related Accession (Same CDS sequence) XM_010823385.2 , XM_010814031.2
    Accession Version XM_010814031.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAACA AAAGCGTGGC
    CCTGGCTTTA AGTACGCCTC CAACCTGCCT GGCTCCCTGC TCAAGGAGCA GAAGATGAAC
    CGGGACAACA AGGAAGGGCA GGAGCCTGCC AAGCGGAGGA GTGAGTGTGA GGAGGCGCCC
    CGGCGCAGGT CCGACGAGCA CCCCAAGAAG GTGCCCCCCG ACGGCATCCT GCGCCGAAAG
    TCGGACGACG TGCACCTGAG GCGGAAGCGG AAATACGAGA AGCCCCAGGA GCTGAGTGGA
    CGCAAGCGAG CCTCGACGCT TCAAACGTCC CCCGGTTCCT CCTCTCACCT CTCGCCGAGG
    CCCCCTCTAG GCAGCAGCCT CAGCCCCTGG TGGAGATCCA GTCTCACTTA CTTCCAGCAG
    CAGCTAAAAC CTGGCAAAGA AGATAAGCTT TTCAGGAAAA AGCGACGGTC CTGGAAGAAC
    GCCGAGGACC GGATAACTCT AGCCAACAAG CCTCTCCGGC GGTTCAAGCA GGAACCAGAG
    GACGACCTAC CCGAGGCGCC CCCCAAGACC AGGGAGAGCG ACCACTCGCG CTCCAGCTCC
    CCTACTGCCG GGCCAAGCAC AGAGGGCGCG GAGGGGCCCG AGGAGAAGAA GAAGGTGAAG
    ATGCGCCGGA AGCGGCGGCT CCCCAACAAG GAGCTGAGCA AGGAGCTGAG CAAGGAGCTG
    AACCAGGAGA TCCAGAAGAC GGAGAGCAGC CTGGCCCAGG AGAATCACCA GCCCATCAAG
    TCGGAGCCTG AGAGCGAGAA CGAGGAGCCC AAGCGGCCCC TGGGCCTCTG CGAGCGCCCC
    CACCGCTTCA GCAAGGGGCT CAACGGCACC CCCCGGGAGC TGCGGCACCA GCTGGGGCCC
    GGCCTGCGCA GCCCGCCCCG AGTCATCTCC CGGCCCCCTC CCTCCGTGTC CCCGCCCAAG
    TGCATCCAGA TGGAGCGACA TGTGATCCGG CCGCCCCCCA TCAGCCCCCC GCCCGACTCG
    CTGCCCTTGG ATGACGGGGC AGCCCATGTC ATGCACAGGG AGGTGTGGAT GGCCGTCTTC
    AGCTACCTCA GCCACCAAGA CTTGTGTGTC TGCATGCGGG TCTGCAAGAC CTGGAACCGC
    TGGTGCTGCG ATAAGCGGTT ATGGACCCGC ATCGATTTGA ACCACTGCAA GTCCATCACG
    CCCCTGATGC TAAGCGGCAT CATCCGGCGA CAGCCTGTCT CCCTAGACCT CAGCTGGACC
    AATATCTCCA AGAAACAGCT GAGCTGGCTC ATCAACCGGT TGCCTGGGCT CCGAGACTTG
    GTGCTGTCCG GCTGCTCGTG GATCGCAGTC TCTGCCCTCT GTAGCTCCAG CTGTCCACTG
    CTCCGGACCC TGGATGTCCA GTGGGTAGAA GGGCTGAAGG ACGCCCAGAT GCGGGACCTC
    CTGTCCCCGC CTACAGACAA CAGGCCAGGT CAGATGGACA ATCGGAGTAA GCTCCGGAAC
    ATTGTGGAGC TGCGTCTGGC CGGCCTGGAC ATCACGGACG CCTCCCTGCG GCTTATCATC
    CGCCACATGC CCCTGCTCTC CAAGCTCCAC CTCAGTTACT GTAACCACGT CACTGACCAG
    TCCATCAACC TGCTCACTGC TGTGGGCACC ACCACCCGGG ACTCCTTAAC CGAAATCAAC
    CTGTCAGACT GCAATAAGGT GACTGACCAG TGCCTGTCCT TCTTCAAACG CTGTGGGAAT
    ATCTGTCACA TTGACCTGAG GTACTGCAAG CAAGTTACCA AGGAAGGCTG CGAGCAGTTC
    ATAGCCGAAA TGTCTGTTAG TGTCCAGTTC GGGCAAGTGG AAGAAAAACT CCTGCAAAAA
    CTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812333.1
    CDS209..4717
    Translation

    Target ORF information:

    RefSeq Version XM_010814031.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGGGCACT GTGCCTCCAG CCCCAGGGAG GGAGTGGCGA
    TCTCCCTGCT CCCCGGGGAC AGCAGGCTCA GCGGGAGGAT CAAGGCCTCC GGCCCAGCCA
    GTGCTGCCTC ACACCGCTGT GTGCCTGGTG TGTGGCGAGG CAGGAAAAGA GGACACAGTG
    GAAGAGGAAG AAGGCAAATT TAACCTCATG CTCATGGAAT GCTCCATCTG CAATGAAATC
    ATCCACCCTG GATGCCTTAA GATTAAGGAG TCAGAGGGCG TGGTCAACGA TGAGCTTCCA
    AACTGCTGGG AGTGTCCCAA GTGTAACCAC GCCGGCAAGA CCGGGAAACA AAAGCGTGGC
    CCTGGCTTTA AGTACGCCTC CAACCTGCCT GGCTCCCTGC TCAAGGAGCA GAAGATGAAC
    CGGGACAACA AGGAAGGGCA GGAGCCTGCC AAGCGGAGGA GTGAGTGTGA GGAGGCGCCC
    CGGCGCAGGT CCGACGAGCA CCCCAAGAAG GTGCCCCCCG ACGGCATCCT GCGCCGAAAG
    TCGGACGACG TGCACCTGAG GCGGAAGCGG AAATACGAGA AGCCCCAGGA GCTGAGTGGA
    CGCAAGCGAG CCTCGACGCT TCAAACGTCC CCCGGTTCCT CCTCTCACCT CTCGCCGAGG
    CCCCCTCTAG GCAGCAGCCT CAGCCCCTGG TGGAGATCCA GTCTCACTTA CTTCCAGCAG
    CAGCTAAAAC CTGGCAAAGA AGATAAGCTT TTCAGGAAAA AGCGACGGTC CTGGAAGAAC
    GCCGAGGACC GGATAACTCT AGCCAACAAG CCTCTCCGGC GGTTCAAGCA GGAACCAGAG
    GACGACCTAC CCGAGGCGCC CCCCAAGACC AGGGAGAGCG ACCACTCGCG CTCCAGCTCC
    CCTACTGCCG GGCCAAGCAC AGAGGGCGCG GAGGGGCCCG AGGAGAAGAA GAAGGTGAAG
    ATGCGCCGGA AGCGGCGGCT CCCCAACAAG GAGCTGAGCA AGGAGCTGAG CAAGGAGCTG
    AACCAGGAGA TCCAGAAGAC GGAGAGCAGC CTGGCCCAGG AGAATCACCA GCCCATCAAG
    TCGGAGCCTG AGAGCGAGAA CGAGGAGCCC AAGCGGCCCC TGGGCCTCTG CGAGCGCCCC
    CACCGCTTCA GCAAGGGGCT CAACGGCACC CCCCGGGAGC TGCGGCACCA GCTGGGGCCC
    GGCCTGCGCA GCCCGCCCCG AGTCATCTCC CGGCCCCCTC CCTCCGTGTC CCCGCCCAAG
    TGCATCCAGA TGGAGCGACA TGTGATCCGG CCGCCCCCCA TCAGCCCCCC GCCCGACTCG
    CTGCCCTTGG ATGACGGGGC AGCCCATGTC ATGCACAGGG AGGTGTGGAT GGCCGTCTTC
    AGCTACCTCA GCCACCAAGA CTTGTGTGTC TGCATGCGGG TCTGCAAGAC CTGGAACCGC
    TGGTGCTGCG ATAAGCGGTT ATGGACCCGC ATCGATTTGA ACCACTGCAA GTCCATCACG
    CCCCTGATGC TAAGCGGCAT CATCCGGCGA CAGCCTGTCT CCCTAGACCT CAGCTGGACC
    AATATCTCCA AGAAACAGCT GAGCTGGCTC ATCAACCGGT TGCCTGGGCT CCGAGACTTG
    GTGCTGTCCG GCTGCTCGTG GATCGCAGTC TCTGCCCTCT GTAGCTCCAG CTGTCCACTG
    CTCCGGACCC TGGATGTCCA GTGGGTAGAA GGGCTGAAGG ACGCCCAGAT GCGGGACCTC
    CTGTCCCCGC CTACAGACAA CAGGCCAGGT CAGATGGACA ATCGGAGTAA GCTCCGGAAC
    ATTGTGGAGC TGCGTCTGGC CGGCCTGGAC ATCACGGACG CCTCCCTGCG GCTTATCATC
    CGCCACATGC CCCTGCTCTC CAAGCTCCAC CTCAGTTACT GTAACCACGT CACTGACCAG
    TCCATCAACC TGCTCACTGC TGTGGGCACC ACCACCCGGG ACTCCTTAAC CGAAATCAAC
    CTGTCAGACT GCAATAAGGT GACTGACCAG TGCCTGTCCT TCTTCAAACG CTGTGGGAAT
    ATCTGTCACA TTGACCTGAG GTACTGCAAG CAAGTTACCA AGGAAGGCTG CGAGCAGTTC
    ATAGCCGAAA TGTCTGTTAG TGTCCAGTTC GGGCAAGTGG AAGAAAAACT CCTGCAAAAA
    CTGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55414
    Clone ID Related Accession (Same CDS sequence) XM_010823386.2 , XM_010814032.2
    Accession Version XM_010823386.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAAGCCTA CAAGCAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821688.1
    CDS208..4632
    Translation

    Target ORF information:

    RefSeq Version XM_010823386.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823386.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAAGCCTA CAAGCAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55414
    Clone ID Related Accession (Same CDS sequence) XM_010823386.2 , XM_010814032.2
    Accession Version XM_010814032.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAAGCCTA CAAGCAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812334.1
    CDS208..4632
    Translation

    Target ORF information:

    RefSeq Version XM_010814032.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAAGCCTA CAAGCAAAAG CGTGGCCCTG GCTTTAAGTA CGCCTCCAAC
    CTGCCTGGCT CCCTGCTCAA GGAGCAGAAG ATGAACCGGG ACAACAAGGA AGGGCAGGAG
    CCTGCCAAGC GGAGGAGTGA GTGTGAGGAG GCGCCCCGGC GCAGGTCCGA CGAGCACCCC
    AAGAAGGTGC CCCCCGACGG CATCCTGCGC CGAAAGTCGG ACGACGTGCA CCTGAGGCGG
    AAGCGGAAAT ACGAGAAGCC CCAGGAGCTG AGTGGACGCA AGCGAGCCTC GACGCTTCAA
    ACGTCCCCCG GTTCCTCCTC TCACCTCTCG CCGAGGCCCC CTCTAGGCAG CAGCCTCAGC
    CCCTGGTGGA GATCCAGTCT CACTTACTTC CAGCAGCAGC TAAAACCTGG CAAAGAAGAT
    AAGCTTTTCA GGAAAAAGCG ACGGTCCTGG AAGAACGCCG AGGACCGGAT AACTCTAGCC
    AACAAGCCTC TCCGGCGGTT CAAGCAGGAA CCAGAGGACG ACCTACCCGA GGCGCCCCCC
    AAGACCAGGG AGAGCGACCA CTCGCGCTCC AGCTCCCCTA CTGCCGGGCC AAGCACAGAG
    GGCGCGGAGG GGCCCGAGGA GAAGAAGAAG GTGAAGATGC GCCGGAAGCG GCGGCTCCCC
    AACAAGGAGC TGAGCAAGGA GCTGAGCAAG GAGCTGAACC AGGAGATCCA GAAGACGGAG
    AGCAGCCTGG CCCAGGAGAA TCACCAGCCC ATCAAGTCGG AGCCTGAGAG CGAGAACGAG
    GAGCCCAAGC GGCCCCTGGG CCTCTGCGAG CGCCCCCACC GCTTCAGCAA GGGGCTCAAC
    GGCACCCCCC GGGAGCTGCG GCACCAGCTG GGGCCCGGCC TGCGCAGCCC GCCCCGAGTC
    ATCTCCCGGC CCCCTCCCTC CGTGTCCCCG CCCAAGTGCA TCCAGATGGA GCGACATGTG
    ATCCGGCCGC CCCCCATCAG CCCCCCGCCC GACTCGCTGC CCTTGGATGA CGGGGCAGCC
    CATGTCATGC ACAGGGAGGT GTGGATGGCC GTCTTCAGCT ACCTCAGCCA CCAAGACTTG
    TGTGTCTGCA TGCGGGTCTG CAAGACCTGG AACCGCTGGT GCTGCGATAA GCGGTTATGG
    ACCCGCATCG ATTTGAACCA CTGCAAGTCC ATCACGCCCC TGATGCTAAG CGGCATCATC
    CGGCGACAGC CTGTCTCCCT AGACCTCAGC TGGACCAATA TCTCCAAGAA ACAGCTGAGC
    TGGCTCATCA ACCGGTTGCC TGGGCTCCGA GACTTGGTGC TGTCCGGCTG CTCGTGGATC
    GCAGTCTCTG CCCTCTGTAG CTCCAGCTGT CCACTGCTCC GGACCCTGGA TGTCCAGTGG
    GTAGAAGGGC TGAAGGACGC CCAGATGCGG GACCTCCTGT CCCCGCCTAC AGACAACAGG
    CCAGGTCAGA TGGACAATCG GAGTAAGCTC CGGAACATTG TGGAGCTGCG TCTGGCCGGC
    CTGGACATCA CGGACGCCTC CCTGCGGCTT ATCATCCGCC ACATGCCCCT GCTCTCCAAG
    CTCCACCTCA GTTACTGTAA CCACGTCACT GACCAGTCCA TCAACCTGCT CACTGCTGTG
    GGCACCACCA CCCGGGACTC CTTAACCGAA ATCAACCTGT CAGACTGCAA TAAGGTGACT
    GACCAGTGCC TGTCCTTCTT CAAACGCTGT GGGAATATCT GTCACATTGA CCTGAGGTAC
    TGCAAGCAAG TTACCAAGGA AGGCTGCGAG CAGTTCATAG CCGAAATGTC TGTTAGTGTC
    CAGTTCGGGC AAGTGGAAGA AAAACTCCTG CAAAAACTGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55415
    Clone ID Related Accession (Same CDS sequence) XM_010814033.2 , XM_010823387.2
    Accession Version XM_010814033.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAACAAAA GCGTGGCCCT GGCTTTAAGT ACGCCTCCAA CCTGCCTGGC
    TCCCTGCTCA AGGAGCAGAA GATGAACCGG GACAACAAGG AAGGGCAGGA GCCTGCCAAG
    CGGAGGAGTG AGTGTGAGGA GGCGCCCCGG CGCAGGTCCG ACGAGCACCC CAAGAAGGTG
    CCCCCCGACG GCATCCTGCG CCGAAAGTCG GACGACGTGC ACCTGAGGCG GAAGCGGAAA
    TACGAGAAGC CCCAGGAGCT GAGTGGACGC AAGCGAGCCT CGACGCTTCA AACGTCCCCC
    GGTTCCTCCT CTCACCTCTC GCCGAGGCCC CCTCTAGGCA GCAGCCTCAG CCCCTGGTGG
    AGATCCAGTC TCACTTACTT CCAGCAGCAG CTAAAACCTG GCAAAGAAGA TAAGCTTTTC
    AGGAAAAAGC GACGGTCCTG GAAGAACGCC GAGGACCGGA TAACTCTAGC CAACAAGCCT
    CTCCGGCGGT TCAAGCAGGA ACCAGAGGAC GACCTACCCG AGGCGCCCCC CAAGACCAGG
    GAGAGCGACC ACTCGCGCTC CAGCTCCCCT ACTGCCGGGC CAAGCACAGA GGGCGCGGAG
    GGGCCCGAGG AGAAGAAGAA GGTGAAGATG CGCCGGAAGC GGCGGCTCCC CAACAAGGAG
    CTGAGCAAGG AGCTGAGCAA GGAGCTGAAC CAGGAGATCC AGAAGACGGA GAGCAGCCTG
    GCCCAGGAGA ATCACCAGCC CATCAAGTCG GAGCCTGAGA GCGAGAACGA GGAGCCCAAG
    CGGCCCCTGG GCCTCTGCGA GCGCCCCCAC CGCTTCAGCA AGGGGCTCAA CGGCACCCCC
    CGGGAGCTGC GGCACCAGCT GGGGCCCGGC CTGCGCAGCC CGCCCCGAGT CATCTCCCGG
    CCCCCTCCCT CCGTGTCCCC GCCCAAGTGC ATCCAGATGG AGCGACATGT GATCCGGCCG
    CCCCCCATCA GCCCCCCGCC CGACTCGCTG CCCTTGGATG ACGGGGCAGC CCATGTCATG
    CACAGGGAGG TGTGGATGGC CGTCTTCAGC TACCTCAGCC ACCAAGACTT GTGTGTCTGC
    ATGCGGGTCT GCAAGACCTG GAACCGCTGG TGCTGCGATA AGCGGTTATG GACCCGCATC
    GATTTGAACC ACTGCAAGTC CATCACGCCC CTGATGCTAA GCGGCATCAT CCGGCGACAG
    CCTGTCTCCC TAGACCTCAG CTGGACCAAT ATCTCCAAGA AACAGCTGAG CTGGCTCATC
    AACCGGTTGC CTGGGCTCCG AGACTTGGTG CTGTCCGGCT GCTCGTGGAT CGCAGTCTCT
    GCCCTCTGTA GCTCCAGCTG TCCACTGCTC CGGACCCTGG ATGTCCAGTG GGTAGAAGGG
    CTGAAGGACG CCCAGATGCG GGACCTCCTG TCCCCGCCTA CAGACAACAG GCCAGGTCAG
    ATGGACAATC GGAGTAAGCT CCGGAACATT GTGGAGCTGC GTCTGGCCGG CCTGGACATC
    ACGGACGCCT CCCTGCGGCT TATCATCCGC CACATGCCCC TGCTCTCCAA GCTCCACCTC
    AGTTACTGTA ACCACGTCAC TGACCAGTCC ATCAACCTGC TCACTGCTGT GGGCACCACC
    ACCCGGGACT CCTTAACCGA AATCAACCTG TCAGACTGCA ATAAGGTGAC TGACCAGTGC
    CTGTCCTTCT TCAAACGCTG TGGGAATATC TGTCACATTG ACCTGAGGTA CTGCAAGCAA
    GTTACCAAGG AAGGCTGCGA GCAGTTCATA GCCGAAATGT CTGTTAGTGT CCAGTTCGGG
    CAAGTGGAAG AAAAACTCCT GCAAAAACTG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812335.1
    CDS207..4622
    Translation

    Target ORF information:

    RefSeq Version XM_010814033.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814033.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAACAAAA GCGTGGCCCT GGCTTTAAGT ACGCCTCCAA CCTGCCTGGC
    TCCCTGCTCA AGGAGCAGAA GATGAACCGG GACAACAAGG AAGGGCAGGA GCCTGCCAAG
    CGGAGGAGTG AGTGTGAGGA GGCGCCCCGG CGCAGGTCCG ACGAGCACCC CAAGAAGGTG
    CCCCCCGACG GCATCCTGCG CCGAAAGTCG GACGACGTGC ACCTGAGGCG GAAGCGGAAA
    TACGAGAAGC CCCAGGAGCT GAGTGGACGC AAGCGAGCCT CGACGCTTCA AACGTCCCCC
    GGTTCCTCCT CTCACCTCTC GCCGAGGCCC CCTCTAGGCA GCAGCCTCAG CCCCTGGTGG
    AGATCCAGTC TCACTTACTT CCAGCAGCAG CTAAAACCTG GCAAAGAAGA TAAGCTTTTC
    AGGAAAAAGC GACGGTCCTG GAAGAACGCC GAGGACCGGA TAACTCTAGC CAACAAGCCT
    CTCCGGCGGT TCAAGCAGGA ACCAGAGGAC GACCTACCCG AGGCGCCCCC CAAGACCAGG
    GAGAGCGACC ACTCGCGCTC CAGCTCCCCT ACTGCCGGGC CAAGCACAGA GGGCGCGGAG
    GGGCCCGAGG AGAAGAAGAA GGTGAAGATG CGCCGGAAGC GGCGGCTCCC CAACAAGGAG
    CTGAGCAAGG AGCTGAGCAA GGAGCTGAAC CAGGAGATCC AGAAGACGGA GAGCAGCCTG
    GCCCAGGAGA ATCACCAGCC CATCAAGTCG GAGCCTGAGA GCGAGAACGA GGAGCCCAAG
    CGGCCCCTGG GCCTCTGCGA GCGCCCCCAC CGCTTCAGCA AGGGGCTCAA CGGCACCCCC
    CGGGAGCTGC GGCACCAGCT GGGGCCCGGC CTGCGCAGCC CGCCCCGAGT CATCTCCCGG
    CCCCCTCCCT CCGTGTCCCC GCCCAAGTGC ATCCAGATGG AGCGACATGT GATCCGGCCG
    CCCCCCATCA GCCCCCCGCC CGACTCGCTG CCCTTGGATG ACGGGGCAGC CCATGTCATG
    CACAGGGAGG TGTGGATGGC CGTCTTCAGC TACCTCAGCC ACCAAGACTT GTGTGTCTGC
    ATGCGGGTCT GCAAGACCTG GAACCGCTGG TGCTGCGATA AGCGGTTATG GACCCGCATC
    GATTTGAACC ACTGCAAGTC CATCACGCCC CTGATGCTAA GCGGCATCAT CCGGCGACAG
    CCTGTCTCCC TAGACCTCAG CTGGACCAAT ATCTCCAAGA AACAGCTGAG CTGGCTCATC
    AACCGGTTGC CTGGGCTCCG AGACTTGGTG CTGTCCGGCT GCTCGTGGAT CGCAGTCTCT
    GCCCTCTGTA GCTCCAGCTG TCCACTGCTC CGGACCCTGG ATGTCCAGTG GGTAGAAGGG
    CTGAAGGACG CCCAGATGCG GGACCTCCTG TCCCCGCCTA CAGACAACAG GCCAGGTCAG
    ATGGACAATC GGAGTAAGCT CCGGAACATT GTGGAGCTGC GTCTGGCCGG CCTGGACATC
    ACGGACGCCT CCCTGCGGCT TATCATCCGC CACATGCCCC TGCTCTCCAA GCTCCACCTC
    AGTTACTGTA ACCACGTCAC TGACCAGTCC ATCAACCTGC TCACTGCTGT GGGCACCACC
    ACCCGGGACT CCTTAACCGA AATCAACCTG TCAGACTGCA ATAAGGTGAC TGACCAGTGC
    CTGTCCTTCT TCAAACGCTG TGGGAATATC TGTCACATTG ACCTGAGGTA CTGCAAGCAA
    GTTACCAAGG AAGGCTGCGA GCAGTTCATA GCCGAAATGT CTGTTAGTGT CCAGTTCGGG
    CAAGTGGAAG AAAAACTCCT GCAAAAACTG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55415
    Clone ID Related Accession (Same CDS sequence) XM_010814033.2 , XM_010823387.2
    Accession Version XM_010823387.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAACAAAA GCGTGGCCCT GGCTTTAAGT ACGCCTCCAA CCTGCCTGGC
    TCCCTGCTCA AGGAGCAGAA GATGAACCGG GACAACAAGG AAGGGCAGGA GCCTGCCAAG
    CGGAGGAGTG AGTGTGAGGA GGCGCCCCGG CGCAGGTCCG ACGAGCACCC CAAGAAGGTG
    CCCCCCGACG GCATCCTGCG CCGAAAGTCG GACGACGTGC ACCTGAGGCG GAAGCGGAAA
    TACGAGAAGC CCCAGGAGCT GAGTGGACGC AAGCGAGCCT CGACGCTTCA AACGTCCCCC
    GGTTCCTCCT CTCACCTCTC GCCGAGGCCC CCTCTAGGCA GCAGCCTCAG CCCCTGGTGG
    AGATCCAGTC TCACTTACTT CCAGCAGCAG CTAAAACCTG GCAAAGAAGA TAAGCTTTTC
    AGGAAAAAGC GACGGTCCTG GAAGAACGCC GAGGACCGGA TAACTCTAGC CAACAAGCCT
    CTCCGGCGGT TCAAGCAGGA ACCAGAGGAC GACCTACCCG AGGCGCCCCC CAAGACCAGG
    GAGAGCGACC ACTCGCGCTC CAGCTCCCCT ACTGCCGGGC CAAGCACAGA GGGCGCGGAG
    GGGCCCGAGG AGAAGAAGAA GGTGAAGATG CGCCGGAAGC GGCGGCTCCC CAACAAGGAG
    CTGAGCAAGG AGCTGAGCAA GGAGCTGAAC CAGGAGATCC AGAAGACGGA GAGCAGCCTG
    GCCCAGGAGA ATCACCAGCC CATCAAGTCG GAGCCTGAGA GCGAGAACGA GGAGCCCAAG
    CGGCCCCTGG GCCTCTGCGA GCGCCCCCAC CGCTTCAGCA AGGGGCTCAA CGGCACCCCC
    CGGGAGCTGC GGCACCAGCT GGGGCCCGGC CTGCGCAGCC CGCCCCGAGT CATCTCCCGG
    CCCCCTCCCT CCGTGTCCCC GCCCAAGTGC ATCCAGATGG AGCGACATGT GATCCGGCCG
    CCCCCCATCA GCCCCCCGCC CGACTCGCTG CCCTTGGATG ACGGGGCAGC CCATGTCATG
    CACAGGGAGG TGTGGATGGC CGTCTTCAGC TACCTCAGCC ACCAAGACTT GTGTGTCTGC
    ATGCGGGTCT GCAAGACCTG GAACCGCTGG TGCTGCGATA AGCGGTTATG GACCCGCATC
    GATTTGAACC ACTGCAAGTC CATCACGCCC CTGATGCTAA GCGGCATCAT CCGGCGACAG
    CCTGTCTCCC TAGACCTCAG CTGGACCAAT ATCTCCAAGA AACAGCTGAG CTGGCTCATC
    AACCGGTTGC CTGGGCTCCG AGACTTGGTG CTGTCCGGCT GCTCGTGGAT CGCAGTCTCT
    GCCCTCTGTA GCTCCAGCTG TCCACTGCTC CGGACCCTGG ATGTCCAGTG GGTAGAAGGG
    CTGAAGGACG CCCAGATGCG GGACCTCCTG TCCCCGCCTA CAGACAACAG GCCAGGTCAG
    ATGGACAATC GGAGTAAGCT CCGGAACATT GTGGAGCTGC GTCTGGCCGG CCTGGACATC
    ACGGACGCCT CCCTGCGGCT TATCATCCGC CACATGCCCC TGCTCTCCAA GCTCCACCTC
    AGTTACTGTA ACCACGTCAC TGACCAGTCC ATCAACCTGC TCACTGCTGT GGGCACCACC
    ACCCGGGACT CCTTAACCGA AATCAACCTG TCAGACTGCA ATAAGGTGAC TGACCAGTGC
    CTGTCCTTCT TCAAACGCTG TGGGAATATC TGTCACATTG ACCTGAGGTA CTGCAAGCAA
    GTTACCAAGG AAGGCTGCGA GCAGTTCATA GCCGAAATGT CTGTTAGTGT CCAGTTCGGG
    CAAGTGGAAG AAAAACTCCT GCAAAAACTG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821689.1
    CDS207..4622
    Translation

    Target ORF information:

    RefSeq Version XM_010823387.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2B (KDM2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823387.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGGGAGG TCGTTTTCGG GGGTGCCAGG CCCCGGGGGC TTTCACTCCA GCAGGCTCGG 
    CTTGCATTTC CGCGCCTCCC GCTGAGGCCA GCCGTGCAAA TCTGCACCTC CCTCCCCACC
    CCCTCCCTCC TTCCCTTCCT CCCTGCCCCG CCATCTCCGG GATGCGCCCG CCGCCTGCAA
    CTCAGCCCTT CCAAAAGTCA GCTCTGCACA CAACTCCCGG GCGGCGGCGG CGCCTGCGTG
    CAGAGGCGCA CTCGTGACCC AACAACAAAA CAGCGATGCC AGGGCGCTAA CGGCGCCCGG
    TGCCTCCCGG GCGCCCTCCC CGCCCCACAC CTCCTCGGGC CGCCGCCCCC TCCCGCCCCC
    ATCCCGGGCC GAGTGCCCCC TCCCTCTACT AGCCCGGCCG CGGGCTCCGA GCGCGATCGG
    CAACAATGTT TACGTTCCGG GGATTATGAC GTCGACGCCT CCCCCCCCAC AACTGCTGAA
    AATGGTTTCC TAGGACTTTG CAAACGGATC TGCTTAAGTC GCCCGATTGA CCGCCAAAGA
    TACGACGAGA ACGAGGACTT GTCGGACGTG GAGGAGATCG TCAGCGCCCG CGGCTTCAGC
    CTGGAGGAAA AGCTGCGCAG CCAGCTGTAC CAGGGGGATT TCGTGCACGC CATGGAGGGC
    AAAGATTTCA ACTATGAGTA TGTGCAGAGA GAAGCTCTCA GAGTTCCCCT GGTATTTCGA
    GAAAAGGATG GCCTGGGAAT TAAGATGCCT GACCCTGATT TCACAGTCCG AGATGTCAAA
    CTCCTAGTGG GTAGCCGACG GCTGGTGGAC GTGATGGACG TGAACACCCA GAAAGGCACG
    GAGATGAGCA TGTCCCAGTT CGTACGGTAC TACGAGACGC CCGAGGCCCA GCGGGACAAG
    CTGTACAACG TCATCAGCCT TGAGTTCAGC CACACCAAGC TGGAGCACTT GGTCAAGCGT
    CCCACCGTGG TAGACCTGGT GGACTGGGTG GACAACATGT GGCCTCAGCA CTTGAAGGAG
    AAGCAGACGG AAGCCACGAA TGCCATTGCA GAGATGAAGT ACCCGAAAGT GAAGAAGTAC
    TGTCTGATGA GTGTGAAGGG TTGTTTCACT GACTTCCACA TCGACTTTGG AGGTACTTCC
    GTTTGGTACC ATGTTTTCCG GGGTGGGAAG ATTTTTTGGC TGATTCCTCC AACCTTGCAC
    AATTTGGCAC TGTATGAGGA GTGGGTGCTG TCAGGCAAAC AGAGCGACAT CTTCCTGGGA
    GACCGCGTGG AGCGATGCCA AAGAATTGAG CTGAAACAGG GTTACACGTT TTTCATCCCT
    TCCGGCTGGA TCCATGCAGT CTACACTCCC GTAGACTCTC TGGTGTTCGG CGGGAACATC
    CTGCACAGCT TCAACGTGCC CATGCAGCTG CGGATCTACG AGATCGAGGA CAGAACGCGG
    GTGCAGCCCA AATTCCGTTA CCCCTTCTAC TATGAGATGT GCTGGTATGT CTTGGAGAGA
    TACGTGTACT GTATGACCCA GCGGTCCTAC CTCACTCAGG AATACCAGAG AGAATCAATG
    CTTATTGATG CCCCAAGAAA GCCCAGCATA GACGGCTTCT CATCTGATTC CTGGCTGGAG
    ATGGAGGAGG AGTCCTGTGA GCAGCAGCCC CAGGAGGAGA AGGAGGAAGA GGAGGAAGAA
    GAGGAAGAGG AGGAGGGTGC AGATAAGACC CCCAGAGCAT CCACCGATGG CCCTGCCTCA
    CCCACGAGCA CCCCGTCCGA GGACCAGGAG GCCCCCGGGA AGAAGCCCAA AGCCCCTGCC
    ATGCGGTTCC TCAAAAGGAC TCTGTCAAAC GAGTCAGAGG ACAGCGTGAA GTCTGCCACG
    ATGCCCACTG ACTACCCCAA GACGCCCACC GGCTCTCCTG CCACTGAGGT CTCTGCCAAA
    TGGACCCACC TCACCGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTTGAA
    TCCCTCCCGG AGAACAAGAA GTGTGTCCCT GAGGGCATCG AGGACCCCCA GGCCCTCCTG
    GAGGGTGTGA AGAATGTCCT GAAGGAGCAC GCGGAGGACG ACCCCAGTCT GGCCATCACT
    GGGGTCCCCG TAGTCACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCGGT AGGTCGGCCC
    AAGGGGAAGC TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC CACGGCGGGA
    GCTCGCCGGC GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC GGAGTGCGGC
    GAGTGCCACT TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGGAT GAAGCAGAGC
    TGCATCATGA GGCAGTGCAT CGCGCCAGTG CTGCCTCACA CCGCTGTGTG CCTGGTGTGT
    GGCGAGGCAG GAAAAGAGGA CACAGTGGAA GAGGAAGAAG GCAAATTTAA CCTCATGCTC
    ATGGAATGCT CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA
    GAGGGCGTGG TCAACGATGA GCTTCCAAAC TGCTGGGAGT GTCCCAAGTG TAACCACGCC
    GGCAAGACCG GGAAACAAAA GCGTGGCCCT GGCTTTAAGT ACGCCTCCAA CCTGCCTGGC
    TCCCTGCTCA AGGAGCAGAA GATGAACCGG GACAACAAGG AAGGGCAGGA GCCTGCCAAG
    CGGAGGAGTG AGTGTGAGGA GGCGCCCCGG CGCAGGTCCG ACGAGCACCC CAAGAAGGTG
    CCCCCCGACG GCATCCTGCG CCGAAAGTCG GACGACGTGC ACCTGAGGCG GAAGCGGAAA
    TACGAGAAGC CCCAGGAGCT GAGTGGACGC AAGCGAGCCT CGACGCTTCA AACGTCCCCC
    GGTTCCTCCT CTCACCTCTC GCCGAGGCCC CCTCTAGGCA GCAGCCTCAG CCCCTGGTGG
    AGATCCAGTC TCACTTACTT CCAGCAGCAG CTAAAACCTG GCAAAGAAGA TAAGCTTTTC
    AGGAAAAAGC GACGGTCCTG GAAGAACGCC GAGGACCGGA TAACTCTAGC CAACAAGCCT
    CTCCGGCGGT TCAAGCAGGA ACCAGAGGAC GACCTACCCG AGGCGCCCCC CAAGACCAGG
    GAGAGCGACC ACTCGCGCTC CAGCTCCCCT ACTGCCGGGC CAAGCACAGA GGGCGCGGAG
    GGGCCCGAGG AGAAGAAGAA GGTGAAGATG CGCCGGAAGC GGCGGCTCCC CAACAAGGAG
    CTGAGCAAGG AGCTGAGCAA GGAGCTGAAC CAGGAGATCC AGAAGACGGA GAGCAGCCTG
    GCCCAGGAGA ATCACCAGCC CATCAAGTCG GAGCCTGAGA GCGAGAACGA GGAGCCCAAG
    CGGCCCCTGG GCCTCTGCGA GCGCCCCCAC CGCTTCAGCA AGGGGCTCAA CGGCACCCCC
    CGGGAGCTGC GGCACCAGCT GGGGCCCGGC CTGCGCAGCC CGCCCCGAGT CATCTCCCGG
    CCCCCTCCCT CCGTGTCCCC GCCCAAGTGC ATCCAGATGG AGCGACATGT GATCCGGCCG
    CCCCCCATCA GCCCCCCGCC CGACTCGCTG CCCTTGGATG ACGGGGCAGC CCATGTCATG
    CACAGGGAGG TGTGGATGGC CGTCTTCAGC TACCTCAGCC ACCAAGACTT GTGTGTCTGC
    ATGCGGGTCT GCAAGACCTG GAACCGCTGG TGCTGCGATA AGCGGTTATG GACCCGCATC
    GATTTGAACC ACTGCAAGTC CATCACGCCC CTGATGCTAA GCGGCATCAT CCGGCGACAG
    CCTGTCTCCC TAGACCTCAG CTGGACCAAT ATCTCCAAGA AACAGCTGAG CTGGCTCATC
    AACCGGTTGC CTGGGCTCCG AGACTTGGTG CTGTCCGGCT GCTCGTGGAT CGCAGTCTCT
    GCCCTCTGTA GCTCCAGCTG TCCACTGCTC CGGACCCTGG ATGTCCAGTG GGTAGAAGGG
    CTGAAGGACG CCCAGATGCG GGACCTCCTG TCCCCGCCTA CAGACAACAG GCCAGGTCAG
    ATGGACAATC GGAGTAAGCT CCGGAACATT GTGGAGCTGC GTCTGGCCGG CCTGGACATC
    ACGGACGCCT CCCTGCGGCT TATCATCCGC CACATGCCCC TGCTCTCCAA GCTCCACCTC
    AGTTACTGTA ACCACGTCAC TGACCAGTCC ATCAACCTGC TCACTGCTGT GGGCACCACC
    ACCCGGGACT CCTTAACCGA AATCAACCTG TCAGACTGCA ATAAGGTGAC TGACCAGTGC
    CTGTCCTTCT TCAAACGCTG TGGGAATATC TGTCACATTG ACCTGAGGTA CTGCAAGCAA
    GTTACCAAGG AAGGCTGCGA GCAGTTCATA GCCGAAATGT CTGTTAGTGT CCAGTTCGGG
    CAAGTGGAAG AAAAACTCCT GCAAAAACTG AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.