THBS4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS4
Entrez Gene ID	541281
Full Name	thrombospondin 4
Synonyms	TSP-4
General protein information	Preferred Names thrombospondin 4 Names thrombospondin-4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034728.1	NP_001029900.1	thrombospondin-4 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THBS4	XP_003310731.1
	THBS4	XP_001109898.1
Mus musculus (house mouse)	Thbs4	NP_035712.1
Danio rerio (zebrafish)	thbs4b	NP_775333.1
Xenopus tropicalis (tropical clawed frog)	thbs4	NP_001072673.1
Canis lupus familiaris (dog)	THBS4	XP_005618229.1
Rattus norvegicus (Norway rat)	Thbs4	NP_058829.1
Gallus gallus (chicken)	THBS4	XP_424763.4
Homo sapiens (human)	THBS4	NP_003239.2
Bos taurus (cattle)	THBS4	NP_001029900.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The C-terminal peptide of thrombospondin-4 stimulates erythroid cell proliferation.
Congote LF, Difalco MR, Gibbs BF
Biochemical and biophysical research communications324(2)673-8(2004 Nov)

Distribution of thrombospondin-4 in the bovine eye.
Si Z, Palkama A, Gebhardt BM, Velasquez D, Galeano MJ, Beuerman RW
Current eye research27(3)165-73(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

thrombospondin-4 C-terminal peptide has a role in stimulating erythroid cell proliferation
Title: The C-terminal peptide of thrombospondin-4 stimulates erythroid cell proliferation.

Thrombospondin-4 widely distributed in bovine eye where it may play role in functions of basement membranes in various tissues. Abundant in trabecular and uveo-scleral pathways. May play role in regulation of aqueous outflow resistance.
Title: Distribution of thrombospondin-4 in the bovine eye.

The following THBS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42613	NM_001034728.1 Latest version!	Bos taurus thrombospondin 4 (THBS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS4 ( NM_001034728.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029900.1 Bos taurus thrombospondin 4 (THBS4), mRNA.

Price & Availability↑

CloneID	OBa42613
Clone ID Related Accession (Same CDS sequence)	NM_001034728.1
Accession Version	NM_001034728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	thrombospondin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104630.1. On or before Oct 18, 2005 this sequence version replaced XM_872773.1, XM_872873.1, XM_615479.2. ##Evidence-Data-START## Transcript exon combination :: BC104630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTTGGCCC CGCGCGGAGC CACCTTCCTC CTGCTGCACC TGGCCCTGCA GCCCTGGCTG 
GGGGCCGGCG CCCAGGCCAC CCCGCAGGTC TTTGACCTTC TCCCATCTGC CAGCCAGAGG 
CTGAACCCGA GTGTTCTGCA GCCGATCCTG ACAGACCCCA CTCTGAACGA GCTCTACGTG 
ATCTCCACCT TCAAGCTGCA GTCGAAAAGC TCAGCCACCA TCTTCGGCCT TTACTCTTCA 
GCTGATCACA GCAAATATTT TGAATTTACT GTCATGGGAC GTTTAAATAA AGCCATCCTC 
CGTTACCTGA AGAACGACGG GAGGATTCAC CTGGTGGTTT TCAACAACCT GCAGCTGGCT 
GACGGCAGGA GGCACAGGCT GCTGCTGAGA CTGACCAACC TCCACCGGGG GGCCGGCTCC 
GTAGAGCTGT TCCTGGACTG CACACGCGTG GACTCCATCC ACAACCTCCC CAGAGCCTTT 
TCCGGCCTCG CCCAGAGTCC TGAGGCTGTG GAACTGAGGA CTTTCCAGAG GAAGGCACAC 
GACTCTTTGG AAGAGCTGAA GCTGGTGGTG AGAGGCTCGC TGATCCAGGT GGCCAGCCTG 
CAAGACTGCT TCCTGCAGCA GAGCGAGCCA CTGGCCACCA CAAACACAGG AGACTTTAAT 
CGGCAGTTCT TGGGGCAGAT GTCACAACTA AACCAACTAC TGGGAGAGGT GAAGGATCTT 
CTGAGACAGC AGGTCAAGGA AACATCATTT TTGCGAAACA CCATAGCCGA ATGCCAGGCT 
TGCGGTCCTC TCAGCTTTCA GTCTCCAACC CCCAACACTC TGATGCCTGT GGTACCCGCG 
GCATCCCCGA CACCCCCCGT GCGCCGCTGT GATTCCAACC CGTGTTTCCG CGGCGTGCGA 
TGCACCGACA CCAGAGATGG CTTTCAGTGC GGACCCTGCC CCGAGGGCTA CACAGGAAAT 
GGGATCGTCT GTTCCGATGT CGATGAGTGT AGGTACCACC CCTGCTATCC TGGCGTGCGC 
TGTGTAAACC TGGCTCCTGG CTTCAGATGT GATGCCTGCC CGGTGGGCTT CACGGGGCCC 
ATGATGCAGG GCGTTGGGAT CAGTTTCGCC AAGACAAACA AGCAGGTCTG CACTGATATT 
GATGAGTGTC GAAACGGAGC ATGTGTTCTT AATTCTATCT GTATTAACAC TTTGGGATCT 
TATCGCTGTG GGCCTTGCAA ACCAGGGTAC ATCGGTGATC AGATGAGGGG ATGCAAAATG 
GAAAGAAACT GCAGAGATCC AGAACTGAAC CCTTGCAGTG TGAACGCCCA GTGCATCGAA 
GAGAGGCAGG GGGATGTGAC GTGCGTGTGT GGGGTCGGCT GGGCTGGTGA CGGCTACATC 
TGTGGAAAGG ATGTGGACAT TGACAGTTAC CCTGATGAGG AGCTGCCATG CTCGGCCAGA 
AACTGCAAAA AGGACAACTG CAAGTATGTG CCAAATTCTG GCCAAGAAGA TGCAGACAGG 
GATGGCATTG GAGACGCCTG TGATGATGAT GCTGATGGAG ACGGGATCCT GAATGAGCAG 
GATAACTGTG TCCTGACCCA CAACGTGGAC CAGAGGAACA GTGACAAAGA CATCTTTGGG 
GATGCCTGTG ATAACTGCCG GAACGTGCTG AATAATGACC AGAAAGACAC TGATGGGGAT 
GGGAAAGGAG ATGCCTGTGA TGACGACATG GATGGGGACG GAATAAAAAA CATCCTGGAC 
AACTGCCAGA AAGTTCCCAA TAGTGACCAA GAAGACAGAG ACGGTGACGG TGTGGGGGAT 
GCCTGTGACA GCTGTCCTGA GGTCAGCAAC CCAAACCAGT CTGACGTGGA TAATGATCTG 
GTTGGGGACT CCTGTGACAC CAATCAGGAC AGCGACGGAG ACGGGCACCA GGACAGCACA 
GACAACTGCC CCACCGTCAT CAACAGTGCC CAGCTGGACA CAGACAAGGA TGGCATTGGT 
GACGAGTGTG ACGATGATGA CGACAACGAC GGCATCCCAG ACTTGGTACC CCCTGGCCCG 
GACAACTGCC GGCTCGTCCC CAACCCCGCC CAGGAGGACA GCAACAGCGA CGGAGTGGGA 
GACATCTGTG AGGCAGACTT TGACCAGGAC CAGGTCATTG ATCGGATAGA CGTGTGCCCA 
GAGAACGCAG AGGTCACCCT GACCGACTTC AGGGCCTATC AGACCGTGGT CCTGGACCCT 
GAAGGGGATG CCCAGATCGA CCCCAACTGG GTGGTCCTGA ACCAGGGCAT GGAGATTGTG 
CAGACCATGA ACAGCGATCC TGGCCTGGCA GTGGGGTACA CGGCTTTTAA TGGAGTTGAC 
TTCGAAGGGA CCTTCCACGT CAACACCCAG ACGGACGATG ACTACGCTGG CTTCATCTTT 
GGTTACCAGG ACAGCTCCAG CTTCTACGTG GTCATGTGGA AGCAGACAGA GCAGACATAC 
TGGCAGGCCA CCCCATTCCG AGCTGTCGCG GAACCTGGCA TTCAGCTCAA GGCCGTGAAG 
TCCAAGACGG GTCCAGGGGA GCATCTCCGC AACTCGCTGT GGCACACGGG GGACACCAGT 
GACCAGGTCA GGCTGCTGTG GAAGGACTCC AGGAACGTGG GCTGGAAGGA CAAGGTGTCC 
TACCGCTGGT TCCTGCAGCA CCGGCCCCAG GTGGGCTACA TCAGGGTGCG ATTTTATGAA 
GGCTCTGAGT TGGTGGCTGA TTCTGGGGTC ACCATAGACA CCACCATGCG TGGAGGCCGG 
CTCGGCGTGT TCTGCTTCTC CCAGGAAAAC ATCATTTGGT CCAACCTGAA ATATCGCTGC 
AATGACACCA TCCCAGAGGA CTTCCAAGAA TTTCAGACCC AGAATTTTGA TCGCCTGGAT 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029900.1
CDS	173..3058
Translation	MLAPRGATFLLLHLALQPWLGAGAQATPQVFDLLPSASQRLNPSVLQPILTDPTLNELYVISTFKLQSKSSATIFGLYSSADHSKYFEFTVMGRLNKAILRYLKNDGRIHLVVFNNLQLADGRRHRLLLRLTNLHRGAGSVELFLDCTRVDSIHNLPRAFSGLAQSPEAVELRTFQRKAHDSLEELKLVVRGSLIQVASLQDCFLQQSEPLATTNTGDFNRQFLGQMSQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLSFQSPTPNTLMPVVPAASPTPPVRRCDSNPCFRGVRCTDTRDGFQCGPCPEGYTGNGIVCSDVDECRYHPCYPGVRCVNLAPGFRCDACPVGFTGPMMQGVGISFAKTNKQVCTDIDECRNGACVLNSICINTLGSYRCGPCKPGYIGDQMRGCKMERNCRDPELNPCSVNAQCIEERQGDVTCVCGVGWAGDGYICGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACDDDADGDGILNEQDNCVLTHNVDQRNSDKDIFGDACDNCRNVLNNDQKDTDGDGKGDACDDDMDGDGIKNILDNCQKVPNSDQEDRDGDGVGDACDSCPEVSNPNQSDVDNDLVGDSCDTNQDSDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQEDSNSDGVGDICEADFDQDQVIDRIDVCPENAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHTGDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQNFDRLDK

Target ORF information:

RefSeq Version	NM_001034728.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin 4 (THBS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034728.1

ORF Insert Sequence:

ATGTTGGCCC CGCGCGGAGC CACCTTCCTC CTGCTGCACC TGGCCCTGCA GCCCTGGCTG 
GGGGCCGGCG CCCAGGCCAC CCCGCAGGTC TTTGACCTTC TCCCATCTGC CAGCCAGAGG 
CTGAACCCGA GTGTTCTGCA GCCGATCCTG ACAGACCCCA CTCTGAACGA GCTCTACGTG 
ATCTCCACCT TCAAGCTGCA GTCGAAAAGC TCAGCCACCA TCTTCGGCCT TTACTCTTCA 
GCTGATCACA GCAAATATTT TGAATTTACT GTCATGGGAC GTTTAAATAA AGCCATCCTC 
CGTTACCTGA AGAACGACGG GAGGATTCAC CTGGTGGTTT TCAACAACCT GCAGCTGGCT 
GACGGCAGGA GGCACAGGCT GCTGCTGAGA CTGACCAACC TCCACCGGGG GGCCGGCTCC 
GTAGAGCTGT TCCTGGACTG CACACGCGTG GACTCCATCC ACAACCTCCC CAGAGCCTTT 
TCCGGCCTCG CCCAGAGTCC TGAGGCTGTG GAACTGAGGA CTTTCCAGAG GAAGGCACAC 
GACTCTTTGG AAGAGCTGAA GCTGGTGGTG AGAGGCTCGC TGATCCAGGT GGCCAGCCTG 
CAAGACTGCT TCCTGCAGCA GAGCGAGCCA CTGGCCACCA CAAACACAGG AGACTTTAAT 
CGGCAGTTCT TGGGGCAGAT GTCACAACTA AACCAACTAC TGGGAGAGGT GAAGGATCTT 
CTGAGACAGC AGGTCAAGGA AACATCATTT TTGCGAAACA CCATAGCCGA ATGCCAGGCT 
TGCGGTCCTC TCAGCTTTCA GTCTCCAACC CCCAACACTC TGATGCCTGT GGTACCCGCG 
GCATCCCCGA CACCCCCCGT GCGCCGCTGT GATTCCAACC CGTGTTTCCG CGGCGTGCGA 
TGCACCGACA CCAGAGATGG CTTTCAGTGC GGACCCTGCC CCGAGGGCTA CACAGGAAAT 
GGGATCGTCT GTTCCGATGT CGATGAGTGT AGGTACCACC CCTGCTATCC TGGCGTGCGC 
TGTGTAAACC TGGCTCCTGG CTTCAGATGT GATGCCTGCC CGGTGGGCTT CACGGGGCCC 
ATGATGCAGG GCGTTGGGAT CAGTTTCGCC AAGACAAACA AGCAGGTCTG CACTGATATT 
GATGAGTGTC GAAACGGAGC ATGTGTTCTT AATTCTATCT GTATTAACAC TTTGGGATCT 
TATCGCTGTG GGCCTTGCAA ACCAGGGTAC ATCGGTGATC AGATGAGGGG ATGCAAAATG 
GAAAGAAACT GCAGAGATCC AGAACTGAAC CCTTGCAGTG TGAACGCCCA GTGCATCGAA 
GAGAGGCAGG GGGATGTGAC GTGCGTGTGT GGGGTCGGCT GGGCTGGTGA CGGCTACATC 
TGTGGAAAGG ATGTGGACAT TGACAGTTAC CCTGATGAGG AGCTGCCATG CTCGGCCAGA 
AACTGCAAAA AGGACAACTG CAAGTATGTG CCAAATTCTG GCCAAGAAGA TGCAGACAGG 
GATGGCATTG GAGACGCCTG TGATGATGAT GCTGATGGAG ACGGGATCCT GAATGAGCAG 
GATAACTGTG TCCTGACCCA CAACGTGGAC CAGAGGAACA GTGACAAAGA CATCTTTGGG 
GATGCCTGTG ATAACTGCCG GAACGTGCTG AATAATGACC AGAAAGACAC TGATGGGGAT 
GGGAAAGGAG ATGCCTGTGA TGACGACATG GATGGGGACG GAATAAAAAA CATCCTGGAC 
AACTGCCAGA AAGTTCCCAA TAGTGACCAA GAAGACAGAG ACGGTGACGG TGTGGGGGAT 
GCCTGTGACA GCTGTCCTGA GGTCAGCAAC CCAAACCAGT CTGACGTGGA TAATGATCTG 
GTTGGGGACT CCTGTGACAC CAATCAGGAC AGCGACGGAG ACGGGCACCA GGACAGCACA 
GACAACTGCC CCACCGTCAT CAACAGTGCC CAGCTGGACA CAGACAAGGA TGGCATTGGT 
GACGAGTGTG ACGATGATGA CGACAACGAC GGCATCCCAG ACTTGGTACC CCCTGGCCCG 
GACAACTGCC GGCTCGTCCC CAACCCCGCC CAGGAGGACA GCAACAGCGA CGGAGTGGGA 
GACATCTGTG AGGCAGACTT TGACCAGGAC CAGGTCATTG ATCGGATAGA CGTGTGCCCA 
GAGAACGCAG AGGTCACCCT GACCGACTTC AGGGCCTATC AGACCGTGGT CCTGGACCCT 
GAAGGGGATG CCCAGATCGA CCCCAACTGG GTGGTCCTGA ACCAGGGCAT GGAGATTGTG 
CAGACCATGA ACAGCGATCC TGGCCTGGCA GTGGGGTACA CGGCTTTTAA TGGAGTTGAC 
TTCGAAGGGA CCTTCCACGT CAACACCCAG ACGGACGATG ACTACGCTGG CTTCATCTTT 
GGTTACCAGG ACAGCTCCAG CTTCTACGTG GTCATGTGGA AGCAGACAGA GCAGACATAC 
TGGCAGGCCA CCCCATTCCG AGCTGTCGCG GAACCTGGCA TTCAGCTCAA GGCCGTGAAG 
TCCAAGACGG GTCCAGGGGA GCATCTCCGC AACTCGCTGT GGCACACGGG GGACACCAGT 
GACCAGGTCA GGCTGCTGTG GAAGGACTCC AGGAACGTGG GCTGGAAGGA CAAGGTGTCC 
TACCGCTGGT TCCTGCAGCA CCGGCCCCAG GTGGGCTACA TCAGGGTGCG ATTTTATGAA 
GGCTCTGAGT TGGTGGCTGA TTCTGGGGTC ACCATAGACA CCACCATGCG TGGAGGCCGG 
CTCGGCGTGT TCTGCTTCTC CCAGGAAAAC ATCATTTGGT CCAACCTGAA ATATCGCTGC 
AATGACACCA TCCCAGAGGA CTTCCAAGAA TTTCAGACCC AGAATTTTGA TCGCCTGGAT 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The C-terminal peptide of thrombospondin-4 stimulates erythroid cell proliferation.
Biochemical and biophysical research communications324(2)673-8(2004 Nov)
Congote LF,Difalco MR,Gibbs BF

Distribution of thrombospondin-4 in the bovine eye.
Current eye research27(3)165-73(2003 Sep)
Si Z,Palkama A,Gebhardt BM,Velasquez D,Galeano MJ,Beuerman RW

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THBS4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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