thbs4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	thbs4b
Entrez Gene ID	252850
Full Name	thrombospondin 4b
Synonyms	thbs4,tsp4,zgc:111910
General protein information	Preferred Names thrombospondin 4b Names thrombospondin-4-B Tsp-4a thbs4-B thrombospondin 4 tsp4b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173226.1	NP_775333.1	thrombospondin-4-B precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THBS4	XP_003310731.1
	THBS4	XP_001109898.1
Mus musculus (house mouse)	Thbs4	NP_035712.1
Danio rerio (zebrafish)	thbs4b	NP_775333.1
Xenopus tropicalis (tropical clawed frog)	thbs4	NP_001072673.1
Canis lupus familiaris (dog)	THBS4	XP_005618229.1
Rattus norvegicus (Norway rat)	Thbs4	NP_058829.1
Gallus gallus (chicken)	THBS4	XP_424763.4
Homo sapiens (human)	THBS4	NP_003239.2
Bos taurus (cattle)	THBS4	NP_001029900.1

Gene Ontology
Bibliography

Related articles in PubMed

In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw.
McGurk PD, Swartz ME, Chen JW, Galloway JL, Eberhart JK
PLoS genetics13(12)e1007112(2017 12)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
Subramanian A, Schilling TF
eLife3(2014 Jun)

miR-34b regulates multiciliogenesis during organ formation in zebrafish.
Wang L, Fu C, Fan H, Du T, Dong M, Chen Y, Jin Y, Zhou Y, Deng M, Gu A, Jing Q, Liu T, Zhou Y
Development (Cambridge, England)140(13)2755-64(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tsp4 functions as an ECM scaffold at myotendinous junctions, with potential therapeutic uses in tendon strengthening and repair
Title: Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.

The following thbs4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thbs4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37408	NM_173226.1 Latest version!	Danio rerio thrombospondin 4b (thbs4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thbs4b ( NM_173226.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775333.1 Danio rerio thrombospondin 4b (thbs4b), mRNA.

Price & Availability↑

CloneID	ODa37408
Clone ID Related Accession (Same CDS sequence)	NM_173226.1
Accession Version	NM_173226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	thrombospondin-4-B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY081009.1. ##Evidence-Data-START## Transcript exon combination :: AY081009.1, BC095593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCA CAATGCATCT CCTCACGGCC GTTTCTCTGA TCTTAATGCT CAGCTCAGCA 
AACGCAGAAT CAACCGTGTA TAACCTCTTA ACGTCACCGG ACTGTCTACC GGATCTACTG 
CATGGTGGTT TGGCAGAGCA GGGAGTAACG GAGCTCTATA TTCTCACAAC GTTCCGGATC 
CAACCTGGAA CCGGAAACAC CATATTTAGC CTGTACAATC CACGAGACAA CAGCAAGTAC 
TTTGAATTCT CGGTGTTTGG AAAAGCAAAT AAAGCTATTT TGCGATACCT GCGCAGAGAT 
GGCAGGATGA GTGCGGTCAC CTTTAACAAA CTGAACCTGG CTGACGGCGA GAAGCATCGG 
CTGCTCTTCC ATCTGAAAGG ACTGGAGGTG GGACATCCCG GAGGCTTCCC GCACAGCCAG 
GGAGCGCTAC CTGTGCCTGG GGTGGAGCTA CACCTGGATT GCAGACTGGT GGAGACGCTG 
AGAGATTTAC CAGCTGTGTT TAACGGCCTA AACAATCACC AGGCGGTGGA GCTCAAGACT 
ATGCAGGGGA AAGCACAAGA GGGCTTGGAG GAGCTCAAAC TGGCATATGG CGATTCAGTG 
GAAAATGTTG CATCGCTGCA GGACTGCCAC ACTCAGAGTG ACTCAGTACA GGCTTTAGGG 
CTTAACACGA AACAGCTTAC AACTCAGATG CTGGAGCTAA CAAAGGTCAT TAATGAGCTG 
AAAGACGTTC TTATTCAGCA GGTGAAGGAA ACATCTTTCC TCAGAAACAC CATCTCTGAG 
TGCCAGGCCT GCGGGCTGAG TGGAGCGGAG GTGGTGAAAC CAAAGTGCGC CCCTGGAGTG 
TGTTTCCGTG ATGATATGTG CATAGAAACA GCTGAAGGAG TGGAGTGTGG TCCATGCCCC 
GATGGATACA CTGGAGATGG ATACAGCTGT GACGATGTTG ATGAGTGCCA GTTTAACCCC 
TGTTTTCCTG GTGTGAGGTG TGTGAACATG GCTCCAGGCT TCCGCTGTGA GGCCTGTCCT 
CTTGGATTCA CTGGCAAACC ACTGGAGGGT GTTGGCGTGG CCTATGCACA AACACACAAG 
CAGGTGTGTG ATGATATTGA TGAGTGTAAA GGGCCAGATA ATGGAGGATG CACAGCGAAC 
TCCATCTGTG TGAATTCTGT GGGATCATAT CAATGCGGGC GATGCAAAAC CGGATTCACG 
GGAGACCAGA TCAGAGGTTG TAAGCCAGAG AAAAGCTGCG GAAACCGTCT GCAGAACCCT 
TGCGATCCCA ATGCACAGTG CACAGAGGAG AGAGACGGCA CCATTACATG CCAGTGTGGC 
ATTGGATGGG CTGGAAATGG TTATCTCTGC GGGAAAGACA CTGACATCGA TGGGTACCCT 
GATGAGAGGC TGCGCTGCCG AGACCCAACC TGCAGAAAGG ATAACTGTGT TACCGTTCCA 
AACTCGGGAC AAGAGGACGC AGATGGCGAT GGGAAGGGTG ACGCATGTGA CCCAGATGCA 
GATGGAGATG GAATATTGAA TGAGCAGGAT AACTGCTGGT TGACGCCAAA TATAAATCAG 
CAGAATAGTG ATAAGGACAG CCATGGTGAT GCTTGTGACA ACTGTGTCCG AGTGGATAAC 
CCAGACCAGA GAGACACAGA CTCTGATGGA CTCGGAGATG CATGTGATGA TGATATGGAT 
GGAGATGGGC TGAAGAACTT CCTGGATAAC TGCCAGCGAG TGAAAAATCG TGATCAGCTG 
GATCGTGATG GGGATGGGGT GGGGGATGCA TGCGATAGCT GTCCTGATAT CCCAAATCCA 
AATCAGTCTG ACATTGACAA TGATCTTGTC GGAGACTCTT GTGATACAAA TCAAGACAGT 
GATGGTGATG GACACCAAGA TAGCAAGGAC AACTGCCCGA TGGTAATTAA CAGCTCCCAG 
CTAGACACAG ATAAAGACGG AATAGGAGAT GAATGTGATG ATGACGATGA TAACGACGGT 
ATTCCCGACT CATTGCCTCC AGGACCAGAT AACTGTCGGT TGGTGCCCAA CCCTGAACAG 
ATAGACGACA ACAATGATGG AGTGGGTGAC ATATGTGAAT CCGACTTTGA CCAGGACAAG 
GTAATCGACA GGATCGATAA CTGTCCAGAA AATGCCGAGA TCACACTGAC AGATTTCAGA 
GCCTATCAGA CAGTTGTACT GGATCCGGAG GGTGATGCCC AGATTGACCC CAACTGGGTG 
GTTCTCAACC AGGGAATGGA GATTGTCCAA ACGATGAACA GTGATCCAGG CCTTGCTGTT 
GGTTACACGG CATTTAGCGG TGTTGATTTC GAGGGAACAT TCCATGTAAA TACAGTGACT 
GATGACGACT ATGCCGGGTT CATCTTTGGA TACCAAGATT CCTCCAGTTT CTATGTGGTG 
ATGTGGAAGC AAACAGAACA GACGTACTGG CAGGCCACAC CCTTCAGAGC TGTAGCTGAA 
CCTGGCATTC AGCTGAAGGC AGTGAAGTCC AAGACTGGTC CAGGTGAGCA TTTGAGGAAC 
TCGCTCTGGC ATACAGGTGA CACTAATGAT CAGGTACGTC TCCTGTGGAA GGATCCAAGA 
AACGTTGGTT GGAAGGACAA GGTATCATAC CGCTGGTACT TGCAGCACCG CCCACAAGTT 
GGATACATCA GGGTTCGTTT CTATGAGGGA ACAGAGCTTG TTGCAGATTC TGGGGTGACA 
ATTGATACCA CAATGAGAGG AGGAAGACTG GGAGTGTTCT GCTTTTCTCA GGAGAACATC 
ATCTGGTCAA ACTTGAAGTA CCGCTGCAAT GACACCATCC CAGAGGACTT CCAAGAGTTC 
AGTACACAAC ATGGCATGGA CCCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775333.1
CDS	139..2988
Translation	MAGTMHLLTAVSLILMLSSANAESTVYNLLTSPDCLPDLLHGGLAEQGVTELYILTTFRIQPGTGNTIFSLYNPRDNSKYFEFSVFGKANKAILRYLRRDGRMSAVTFNKLNLADGEKHRLLFHLKGLEVGHPGGFPHSQGALPVPGVELHLDCRLVETLRDLPAVFNGLNNHQAVELKTMQGKAQEGLEELKLAYGDSVENVASLQDCHTQSDSVQALGLNTKQLTTQMLELTKVINELKDVLIQQVKETSFLRNTISECQACGLSGAEVVKPKCAPGVCFRDDMCIETAEGVECGPCPDGYTGDGYSCDDVDECQFNPCFPGVRCVNMAPGFRCEACPLGFTGKPLEGVGVAYAQTHKQVCDDIDECKGPDNGGCTANSICVNSVGSYQCGRCKTGFTGDQIRGCKPEKSCGNRLQNPCDPNAQCTEERDGTITCQCGIGWAGNGYLCGKDTDIDGYPDERLRCRDPTCRKDNCVTVPNSGQEDADGDGKGDACDPDADGDGILNEQDNCWLTPNINQQNSDKDSHGDACDNCVRVDNPDQRDTDSDGLGDACDDDMDGDGLKNFLDNCQRVKNRDQLDRDGDGVGDACDSCPDIPNPNQSDIDNDLVGDSCDTNQDSDGDGHQDSKDNCPMVINSSQLDTDKDGIGDECDDDDDNDGIPDSLPPGPDNCRLVPNPEQIDDNNDGVGDICESDFDQDKVIDRIDNCPENAEITLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFSGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHTGDTNDQVRLLWKDPRNVGWKDKVSYRWYLQHRPQVGYIRVRFYEGTELVADSGVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFSTQHGMDPL

Target ORF information:

RefSeq Version	NM_173226.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin 4b (thbs4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173226.1

ORF Insert Sequence:

ATGGCCGGCA CAATGCATCT CCTCACGGCC GTTTCTCTGA TCTTAATGCT CAGCTCAGCA 
AACGCAGAAT CAACCGTGTA TAACCTCTTA ACGTCACCGG ACTGTCTACC GGATCTACTG 
CATGGTGGTT TGGCAGAGCA GGGAGTAACG GAGCTCTATA TTCTCACAAC GTTCCGGATC 
CAACCTGGAA CCGGAAACAC CATATTTAGC CTGTACAATC CACGAGACAA CAGCAAGTAC 
TTTGAATTCT CGGTGTTTGG AAAAGCAAAT AAAGCTATTT TGCGATACCT GCGCAGAGAT 
GGCAGGATGA GTGCGGTCAC CTTTAACAAA CTGAACCTGG CTGACGGCGA GAAGCATCGG 
CTGCTCTTCC ATCTGAAAGG ACTGGAGGTG GGACATCCCG GAGGCTTCCC GCACAGCCAG 
GGAGCGCTAC CTGTGCCTGG GGTGGAGCTA CACCTGGATT GCAGACTGGT GGAGACGCTG 
AGAGATTTAC CAGCTGTGTT TAACGGCCTA AACAATCACC AGGCGGTGGA GCTCAAGACT 
ATGCAGGGGA AAGCACAAGA GGGCTTGGAG GAGCTCAAAC TGGCATATGG CGATTCAGTG 
GAAAATGTTG CATCGCTGCA GGACTGCCAC ACTCAGAGTG ACTCAGTACA GGCTTTAGGG 
CTTAACACGA AACAGCTTAC AACTCAGATG CTGGAGCTAA CAAAGGTCAT TAATGAGCTG 
AAAGACGTTC TTATTCAGCA GGTGAAGGAA ACATCTTTCC TCAGAAACAC CATCTCTGAG 
TGCCAGGCCT GCGGGCTGAG TGGAGCGGAG GTGGTGAAAC CAAAGTGCGC CCCTGGAGTG 
TGTTTCCGTG ATGATATGTG CATAGAAACA GCTGAAGGAG TGGAGTGTGG TCCATGCCCC 
GATGGATACA CTGGAGATGG ATACAGCTGT GACGATGTTG ATGAGTGCCA GTTTAACCCC 
TGTTTTCCTG GTGTGAGGTG TGTGAACATG GCTCCAGGCT TCCGCTGTGA GGCCTGTCCT 
CTTGGATTCA CTGGCAAACC ACTGGAGGGT GTTGGCGTGG CCTATGCACA AACACACAAG 
CAGGTGTGTG ATGATATTGA TGAGTGTAAA GGGCCAGATA ATGGAGGATG CACAGCGAAC 
TCCATCTGTG TGAATTCTGT GGGATCATAT CAATGCGGGC GATGCAAAAC CGGATTCACG 
GGAGACCAGA TCAGAGGTTG TAAGCCAGAG AAAAGCTGCG GAAACCGTCT GCAGAACCCT 
TGCGATCCCA ATGCACAGTG CACAGAGGAG AGAGACGGCA CCATTACATG CCAGTGTGGC 
ATTGGATGGG CTGGAAATGG TTATCTCTGC GGGAAAGACA CTGACATCGA TGGGTACCCT 
GATGAGAGGC TGCGCTGCCG AGACCCAACC TGCAGAAAGG ATAACTGTGT TACCGTTCCA 
AACTCGGGAC AAGAGGACGC AGATGGCGAT GGGAAGGGTG ACGCATGTGA CCCAGATGCA 
GATGGAGATG GAATATTGAA TGAGCAGGAT AACTGCTGGT TGACGCCAAA TATAAATCAG 
CAGAATAGTG ATAAGGACAG CCATGGTGAT GCTTGTGACA ACTGTGTCCG AGTGGATAAC 
CCAGACCAGA GAGACACAGA CTCTGATGGA CTCGGAGATG CATGTGATGA TGATATGGAT 
GGAGATGGGC TGAAGAACTT CCTGGATAAC TGCCAGCGAG TGAAAAATCG TGATCAGCTG 
GATCGTGATG GGGATGGGGT GGGGGATGCA TGCGATAGCT GTCCTGATAT CCCAAATCCA 
AATCAGTCTG ACATTGACAA TGATCTTGTC GGAGACTCTT GTGATACAAA TCAAGACAGT 
GATGGTGATG GACACCAAGA TAGCAAGGAC AACTGCCCGA TGGTAATTAA CAGCTCCCAG 
CTAGACACAG ATAAAGACGG AATAGGAGAT GAATGTGATG ATGACGATGA TAACGACGGT 
ATTCCCGACT CATTGCCTCC AGGACCAGAT AACTGTCGGT TGGTGCCCAA CCCTGAACAG 
ATAGACGACA ACAATGATGG AGTGGGTGAC ATATGTGAAT CCGACTTTGA CCAGGACAAG 
GTAATCGACA GGATCGATAA CTGTCCAGAA AATGCCGAGA TCACACTGAC AGATTTCAGA 
GCCTATCAGA CAGTTGTACT GGATCCGGAG GGTGATGCCC AGATTGACCC CAACTGGGTG 
GTTCTCAACC AGGGAATGGA GATTGTCCAA ACGATGAACA GTGATCCAGG CCTTGCTGTT 
GGTTACACGG CATTTAGCGG TGTTGATTTC GAGGGAACAT TCCATGTAAA TACAGTGACT 
GATGACGACT ATGCCGGGTT CATCTTTGGA TACCAAGATT CCTCCAGTTT CTATGTGGTG 
ATGTGGAAGC AAACAGAACA GACGTACTGG CAGGCCACAC CCTTCAGAGC TGTAGCTGAA 
CCTGGCATTC AGCTGAAGGC AGTGAAGTCC AAGACTGGTC CAGGTGAGCA TTTGAGGAAC 
TCGCTCTGGC ATACAGGTGA CACTAATGAT CAGGTACGTC TCCTGTGGAA GGATCCAAGA 
AACGTTGGTT GGAAGGACAA GGTATCATAC CGCTGGTACT TGCAGCACCG CCCACAAGTT 
GGATACATCA GGGTTCGTTT CTATGAGGGA ACAGAGCTTG TTGCAGATTC TGGGGTGACA 
ATTGATACCA CAATGAGAGG AGGAAGACTG GGAGTGTTCT GCTTTTCTCA GGAGAACATC 
ATCTGGTCAA ACTTGAAGTA CCGCTGCAAT GACACCATCC CAGAGGACTT CCAAGAGTTC 
AGTACACAAC ATGGCATGGA CCCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw.
PLoS genetics13(12)e1007112(2017 12)
McGurk PD,Swartz ME,Chen JW,Galloway JL,Eberhart JK

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
eLife3(2014 Jun)
Subramanian A,Schilling TF

miR-34b regulates multiciliogenesis during organ formation in zebrafish.
Development (Cambridge, England)140(13)2755-64(2013 Jul)
Wang L,Fu C,Fan H,Du T,Dong M,Chen Y,Jin Y,Zhou Y,Deng M,Gu A,Jing Q,Liu T,Zhou Y

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thbs4b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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