TNRC6A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015469345.1	XP_015324831.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_015469342.1	XP_015324828.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_015460350.1	XP_015315836.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_015469346.1	XP_015324832.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_024985201.1	XP_024840969.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_010819377.2	XP_010817679.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_010819373.2	XP_010817675.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_024985200.1	XP_024840968.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_015469343.1	XP_015324829.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_015460351.1	XP_015315837.1	trinucleotide repeat-containing gene 6A protein isoform X6
XM_010819375.3	XP_010817677.2	trinucleotide repeat-containing gene 6A protein isoform X1
XM_024985202.1	XP_024840970.1	trinucleotide repeat-containing gene 6A protein isoform X6
XM_024985203.1	XP_024840971.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_015469344.1	XP_015324830.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_015469347.1	XP_015324833.1	trinucleotide repeat-containing gene 6A protein isoform X6
XM_010819375.2	XP_010817677.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_010819374.2	XP_010817676.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_024985198.1	XP_024840966.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_024985199.1	XP_024840967.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_024985204.1	XP_024840972.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_024985205.1	XP_024840973.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_015460350.2	XP_015315836.2	trinucleotide repeat-containing gene 6A protein isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230525	XM_010819375.3 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230526	XM_024985198.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230527	XM_024985199.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230528	XM_024985200.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230529	XM_024985201.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230530	XM_024985202.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa230531	XM_024985205.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230531	XM_024985204.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230531	XM_024985203.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230532	XM_015460350.2 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa33026	XM_010819374.2 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54984	XM_010819373.2 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54984	XM_015469344.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54985	XM_010819375.2 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54985	XM_015469345.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54986	XM_015469346.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa54986	XM_010819377.2 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88502	XM_015460350.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88502	XM_015469342.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88503	XM_015469347.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa88503	XM_015460351.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa95212	XM_015469343.1 Latest version!	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

TNRC6A ( XM_010819375.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817677.2 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230525
Clone ID Related Accession (Same CDS sequence)	XM_010819375.3
Accession Version	XM_010819375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817677.2
CDS	493..6192
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_010819375.3
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819375.3

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985198.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840966.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OBa230526
Clone ID Related Accession (Same CDS sequence)	XM_024985198.1
Accession Version	XM_024985198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGAA TGATGAAAGG TGGAAACAAA CAAGAAGAAG CATGGATAAA TCCATTTGTT 
AAACAGTTTT CAAATATCAG TTTTTCGAGA GACTCGCCAG AAGAAAATGT ACAGAGCAAT 
AAGATGGACC TTTCTGGAGG AATGTTACAA GACAAACGAA TGGAGATAGA TAAACATAGC 
CTAAATATTG GTGATTACAA TCGAACGGTC GGGAAAGGCC CTGGTTCTCG GCCTCAGATT 
TCCAAAGAGT CTTCCATGGA CCGCAGTCCT TATTTTGATA AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840966.1
CDS	493..6159
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985198.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985198.1

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGAA TGATGAAAGG TGGAAACAAA CAAGAAGAAG CATGGATAAA TCCATTTGTT 
AAACAGTTTT CAAATATCAG TTTTTCGAGA GACTCGCCAG AAGAAAATGT ACAGAGCAAT 
AAGATGGACC TTTCTGGAGG AATGTTACAA GACAAACGAA TGGAGATAGA TAAACATAGC 
CTAAATATTG GTGATTACAA TCGAACGGTC GGGAAAGGCC CTGGTTCTCG GCCTCAGATT 
TCCAAAGAGT CTTCCATGGA CCGCAGTCCT TATTTTGATA AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985199.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840967.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OBa230527
Clone ID Related Accession (Same CDS sequence)	XM_024985199.1
Accession Version	XM_024985199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTT TATCAGTGCC AGAACAGATA AAGCCCAGTG TAAGCCAGCC TCAGCCTGCC 
AACTCTAATA ACGGCACTTC CACAGCAACC AGCACTAATA ATAATGCCAA GCGAGCCACA 
GCCAACAATC AGCAGCAGCA GCAGCAACAG CCGCAACCGC AGCAGCAGCA ACCGCAGCAG 
CCGCAGCAGC CGCAGCCGCC ACCACAGGCC TTGCCTCGGT ATCCCCGGGA AGTACCACCT 
CGATTTCGCC ACCAGGAACA TAAGCAGCTT CTAAAGAGGG GTCAGCATTT TCCCGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGATCA GCAAAAACCA GTCAGATACA 
AACCACAATA CTCCAGGATC CCATTATGAA AATTCAGAGC GGGGACCTGT GTCGTCTACA 
AGTGACTCTC GTACAAACTG TAAGAATGCT GTTGTGAACG ACTCCCCTGA AAAAGAAGCA 
TGGCCCTCAG CCCCTGGCAG TGATCCGGAG TTGGTTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GCGACCCATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAATGATG GCCTTCGGAA TAGCACTGGA CTTGGATCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCACGGAAGT AGCACTGGGC CGTGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGCCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGAGAA CAACGGACTT 
GCCCTAAAAG GGCCTGTAGG GAGTGGTAGT TCTGGCATCA ATATTCAGTG CAGTCCCTTA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTGCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAAAA TATTACCACT GAAATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAATGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC CTCGGTTGTA 
AATGGCACTT CCCTTTCTCA CCTTAGCAAT GGAGAGTCAA AAAGTGGAGG CTCTTACGGT 
ACTACATGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCAAG CCCTAATGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCGTAAA CGGGCCTATG 
GGCACTAACT TTCAAGTTAA CACAAACAAA GGAGGCGGCG TGTGGGAGTC TGGAGCAGTG 
AATTCCCAGA GTGCATCCTG GGGAAATGGT GCAAATTCTG GAGGAAGTCG AAGAGGGTGG 
GGAACTCCTG CACAAAACAC TGGCACTAAT ACACCCGGCG TGGAGTGGAA CAAACTGCCT 
GGCAACCAGC ATTCCAGTGA CAGTGCAAAT GGCAACGGTA AGAAGTTTAC AAACGGATGG 
AAGTCTACTG AGGAAGAGGA TCAGGGTTCT GCCGCATCTC AGACAAATGA GCAAAGCAGT 
GTGTGGGCCA AAACAGGAGG TACAGTGGAG AGTGAAGGTA GTACAGAAAG CACTGGACGC 
CTCGAGGAGA AAGCGACTGG GGAAAATCAG AGTAGGGACA GAAGGAAGAT GGATCAGCAC 
ACGTTACTCC AAAGCATTGT AAACAGAACT GACTTAGATC CACGTGTCCT GTCCAACTCT 
GGCTGGGGAC AGACTCCTAT TAAGCAGAAT ACTGCCTGGG ATACAGAAAC ATCACCAAGA 
GGGGAGAGAA AGACTGACAA TGGGACAGAG GCCTGGGGAA GCTCTGCAAC ACAGACTTTT 
AACTCAGGGG CGTGTGTAGA TAAGACTAGC CCGAGTAGTA ATGATACCTC ATCTGTATCG 
GGGTGGGGAG ATCCCAAACC TGCTCTGCGG TGGGGAGATT CCAAAGGCTC AAGCTGCCAG 
GGGGGATGGG AAGATGACTC TGCTGCTACA GGAATGGTCA AGAGCAATCA GTGGGGGAAC 
TGCAAAGAAG AGAAGTCCGC ATGGAATGAT TCGCAAAAGA ACAAACAGGG ATGGGGTGAC 
GGACAAAAGT CAAACCAAGG GTGGTCCGTC TCTGCCGGTG ACAACTGGGG AGAAAGGAGT 
AACCATTGGG GAGAGGCTAA CAAGAAGTCT AGCTCAGGAG GTAGTGACAG TGATAGGTCT 
GTTTCTGGTT GGAATGAACT CGGTAAAACC AGTTCTTTTA CATGGGGAAA TAACATTAAT 
CCAAATAATT CATCAGGCTG GGACGAATCT TCTAAACCGA ATCCTTCCCA GGGATGGGGA 
GACCCTCCAA AGTCTAATCA GTCTCTAGGT TGGGGAGATT CATCAAAGCC GGTCAGTTCT 
CCAGACTGGA ACAAGCAACA AGACATTGTC GGATCTTGGG GCATCCCGCC AGCGACAGGC 
AAACCTCCTG GTACAGGCTG GCTGGGAGGA CCTATGCCAG CCCCAGCGAA AGAGGAAGAA 
CCCACGGGCT GGGAGGAGCC ATCCCCAGAA TCCATACGTC GCAAAATGGA GATTGACGAT 
GGGACTTCAG CTTGGGGAGA TCCCAGCAAA TACAATTACA AAAATGTGAA CATGTGGAAC 
AAAAACGTCC CAAATGGCAG CAGTCGCTCA GACCAGCAAG CACAGGTACA CCAGTTGCTA 
CCGTCTGCAG GTACCATCTC AAACAAAGAG GCGAGCAGTG GCTCCGGCTG GGGTGAGCCC 
TGGGGGGAGA CTTCAACTCC AGCCACAACT GTGGATAATG GTACTTCAGC CTGGGGTAAA 
CCCGTAGACA GTGGGCCTAG CTGGGGCGAA CCCATTGCTG CGGCATCCAG CACATCCACG 
TGGGGCTCCA GCTCTGTTGG TCCACAAACA TTAAACAAAT CTGGGCCAAA ATCTATGCAA 
GACGGCTGGT GTGGTGATGA TATGCCCTTG CCTGGAAATC GCCCCACTGG CTGGGAAGAG 
GAAGAAGATG TAGAGATTGG AATGTGGAAT AGTAATTCAT CTCAAGAGCT TAATTCATCT 
TTAAATTGGC CACCATATAC AAAGAAAATG TCTTCGAAGG GTCTGAGTGG CAAAAAAAGG 
AGAAGGGAAA GGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840967.1
CDS	16..5568
Translation	MKFLSVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985199.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985199.1

ORF Insert Sequence:

ATGAAGTTTT TATCAGTGCC AGAACAGATA AAGCCCAGTG TAAGCCAGCC TCAGCCTGCC 
AACTCTAATA ACGGCACTTC CACAGCAACC AGCACTAATA ATAATGCCAA GCGAGCCACA 
GCCAACAATC AGCAGCAGCA GCAGCAACAG CCGCAACCGC AGCAGCAGCA ACCGCAGCAG 
CCGCAGCAGC CGCAGCCGCC ACCACAGGCC TTGCCTCGGT ATCCCCGGGA AGTACCACCT 
CGATTTCGCC ACCAGGAACA TAAGCAGCTT CTAAAGAGGG GTCAGCATTT TCCCGTCATA 
GCAGCAAACC TGGGATCTGC TGTTAAAGTG TTAAGCAGCC AGTCAGAAAG CAGTGCTTTA 
ACAAATCAAC AGCCACAAAA TAACGGAGAG GTGCAGATCA GCAAAAACCA GTCAGATACA 
AACCACAATA CTCCAGGATC CCATTATGAA AATTCAGAGC GGGGACCTGT GTCGTCTACA 
AGTGACTCTC GTACAAACTG TAAGAATGCT GTTGTGAACG ACTCCCCTGA AAAAGAAGCA 
TGGCCCTCAG CCCCTGGCAG TGATCCGGAG TTGGTTTCAG AATGTATGGA TGCTGATTCT 
GCCTCCAGTT CTGAATCAGA GCGACCCATC ACTATCATGG CTTCAGGGAG CACAGGTGGT 
GAAAATGATG GCCTTCGGAA TAGCACTGGA CTTGGATCCC AAAACAAGTT TGTGGTTGGT 
AGCAGCAGCA ATAATGTGGG CCACGGAAGT AGCACTGGGC CGTGGGGTTT TTCCCATGGA 
GCCATAATAA GCACATGTCA GGCCTCTGTG GATGCTCCTG AAAGCAAATC AGAAAGTAGC 
AACAATAGAA TGAATGCTTG GGGCACTGTA AGTTCTTCAT CAAATGGAGG GTTAAATCCA 
AGCACTTTGA ATTCAGCTAG CAACCATGGT GCCTGGCCAG TATTAGAGAA CAACGGACTT 
GCCCTAAAAG GGCCTGTAGG GAGTGGTAGT TCTGGCATCA ATATTCAGTG CAGTCCCTTA 
GGCCAGATGC CTAACAATCA GAGTATTAAC TCTAAAGTGG GTGGTGCTTC CACCCATGGT 
ACCTGGGGAA GCCTTCAGGA AACTTGTGAA TCTGAAGTAA GTGGTACACA GAAGGTTTCA 
TTCAGTGGTC AACCTCAAAA TATTACCACT GAAATGACTG GACCAAATAA CACTACTAAC 
TTTATGACCT CTAGTTTACC AAACTCCGGT TCAGTACAGA ATAATGAACT GCCTAGTAAT 
ACAGGGGCCT GGCGTGTGAG CACAATGAAT CATCCTCAGA TACAGGCTCC CTCGGTTGTA 
AATGGCACTT CCCTTTCTCA CCTTAGCAAT GGAGAGTCAA AAAGTGGAGG CTCTTACGGT 
ACTACATGGG GTGCCTATGG TTCTAATTAC TCTGGAGACA AATGTTCAAG CCCTAATGGC 
CAAGCTAATG GTGACACTGT GAATGCAACT CTAATGCAGC CTGGCGTAAA CGGGCCTATG 
GGCACTAACT TTCAAGTTAA CACAAACAAA GGAGGCGGCG TGTGGGAGTC TGGAGCAGTG 
AATTCCCAGA GTGCATCCTG GGGAAATGGT GCAAATTCTG GAGGAAGTCG AAGAGGGTGG 
GGAACTCCTG CACAAAACAC TGGCACTAAT ACACCCGGCG TGGAGTGGAA CAAACTGCCT 
GGCAACCAGC ATTCCAGTGA CAGTGCAAAT GGCAACGGTA AGAAGTTTAC AAACGGATGG 
AAGTCTACTG AGGAAGAGGA TCAGGGTTCT GCCGCATCTC AGACAAATGA GCAAAGCAGT 
GTGTGGGCCA AAACAGGAGG TACAGTGGAG AGTGAAGGTA GTACAGAAAG CACTGGACGC 
CTCGAGGAGA AAGCGACTGG GGAAAATCAG AGTAGGGACA GAAGGAAGAT GGATCAGCAC 
ACGTTACTCC AAAGCATTGT AAACAGAACT GACTTAGATC CACGTGTCCT GTCCAACTCT 
GGCTGGGGAC AGACTCCTAT TAAGCAGAAT ACTGCCTGGG ATACAGAAAC ATCACCAAGA 
GGGGAGAGAA AGACTGACAA TGGGACAGAG GCCTGGGGAA GCTCTGCAAC ACAGACTTTT 
AACTCAGGGG CGTGTGTAGA TAAGACTAGC CCGAGTAGTA ATGATACCTC ATCTGTATCG 
GGGTGGGGAG ATCCCAAACC TGCTCTGCGG TGGGGAGATT CCAAAGGCTC AAGCTGCCAG 
GGGGGATGGG AAGATGACTC TGCTGCTACA GGAATGGTCA AGAGCAATCA GTGGGGGAAC 
TGCAAAGAAG AGAAGTCCGC ATGGAATGAT TCGCAAAAGA ACAAACAGGG ATGGGGTGAC 
GGACAAAAGT CAAACCAAGG GTGGTCCGTC TCTGCCGGTG ACAACTGGGG AGAAAGGAGT 
AACCATTGGG GAGAGGCTAA CAAGAAGTCT AGCTCAGGAG GTAGTGACAG TGATAGGTCT 
GTTTCTGGTT GGAATGAACT CGGTAAAACC AGTTCTTTTA CATGGGGAAA TAACATTAAT 
CCAAATAATT CATCAGGCTG GGACGAATCT TCTAAACCGA ATCCTTCCCA GGGATGGGGA 
GACCCTCCAA AGTCTAATCA GTCTCTAGGT TGGGGAGATT CATCAAAGCC GGTCAGTTCT 
CCAGACTGGA ACAAGCAACA AGACATTGTC GGATCTTGGG GCATCCCGCC AGCGACAGGC 
AAACCTCCTG GTACAGGCTG GCTGGGAGGA CCTATGCCAG CCCCAGCGAA AGAGGAAGAA 
CCCACGGGCT GGGAGGAGCC ATCCCCAGAA TCCATACGTC GCAAAATGGA GATTGACGAT 
GGGACTTCAG CTTGGGGAGA TCCCAGCAAA TACAATTACA AAAATGTGAA CATGTGGAAC 
AAAAACGTCC CAAATGGCAG CAGTCGCTCA GACCAGCAAG CACAGGTACA CCAGTTGCTA 
CCGTCTGCAG GTACCATCTC AAACAAAGAG GCGAGCAGTG GCTCCGGCTG GGGTGAGCCC 
TGGGGGGAGA CTTCAACTCC AGCCACAACT GTGGATAATG GTACTTCAGC CTGGGGTAAA 
CCCGTAGACA GTGGGCCTAG CTGGGGCGAA CCCATTGCTG CGGCATCCAG CACATCCACG 
TGGGGCTCCA GCTCTGTTGG TCCACAAACA TTAAACAAAT CTGGGCCAAA ATCTATGCAA 
GACGGCTGGT GTGGTGATGA TATGCCCTTG CCTGGAAATC GCCCCACTGG CTGGGAAGAG 
GAAGAAGATG TAGAGATTGG AATGTGGAAT AGTAATTCAT CTCAAGAGCT TAATTCATCT 
TTAAATTGGC CACCATATAC AAAGAAAATG TCTTCGAAGG GTCTGAGTGG CAAAAAAAGG 
AGAAGGGAAA GGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985200.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840968.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

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CloneID	OBa230528
Clone ID Related Accession (Same CDS sequence)	XM_024985200.1
Accession Version	XM_024985200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGGCCAAA ATCTATGCAA GACGGCTGGT GTGGTGATGA TATGCCCTTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAAGATG TAGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAATTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCTTCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCATG GATAAATCCA TTTGTTAAAC AGTTTTCAAA TATCAGTTTT 
TCGAGAGACT CGCCAGAAGA AAATGTACAG AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGACCGC 
AGTCCTTATT TTGATAAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTCA GTTCATCTCA GCCTAGTCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCGCTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAACA AACTCCGCCA TCTCAGCAAC AGTCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGAAAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GCTCCCCTCA GCAGCACAGC ACAAAGCACT GCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCCCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTCTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTT CGTGGGATAA TTCTCCCCTT CGAGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATT GATGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAACGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCAGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCATGGCA CTTCCCTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840968.1
CDS	493..6015
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985200.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985200.1

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGGCCAAA ATCTATGCAA GACGGCTGGT GTGGTGATGA TATGCCCTTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAAGATG TAGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAATTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCTTCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCATG GATAAATCCA TTTGTTAAAC AGTTTTCAAA TATCAGTTTT 
TCGAGAGACT CGCCAGAAGA AAATGTACAG AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGACCGC 
AGTCCTTATT TTGATAAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTCA GTTCATCTCA GCCTAGTCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCGCTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AACTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCAGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAACA AACTCCGCCA TCTCAGCAAC AGTCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGAAAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC TACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGCCCTGGTG AACCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GCTCCCCTCA GCAGCACAGC ACAAAGCACT GCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCCCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTCTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTT CGTGGGATAA TTCTCCCCTT CGAGTAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGGTATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATT GATGGCTCAA CTCTGCGTAC TCTGTGCATG 
CAGCATGGCC CACTGATAAC ATTCCACCTG AACCTCCCAC ATGGAAACGC TTTGGTCCGT 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGTCAGTCTC TGACCCCTTC TCCCAGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCATGGCA CTTCCCTCTG GGGGACCCCA 
CATTATTCCA CAAGCCTGTG GGGTCCTCCC AGCAGCAGCG ACCCCCGGGG AATTAGCAGC 
CCATCCCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985201.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840969.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

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CloneID	OBa230529
Clone ID Related Accession (Same CDS sequence)	XM_024985201.1
Accession Version	XM_024985201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGATACAAA CCACAATACT 
CCAGGATCCC ATTATGAAAA TTCAGAGCGG GGACCTGTGT CGTCTACAAG TGACTCTCGT 
ACAAACTGTA AGAATGCTGT TGTGAACGAC TCCCCTGAAA AAGAAGCATG GCCCTCAGCC 
CCTGGCAGTG ATCCGGAGTT GGTTTCAGAA TGTATGGATG CTGATTCTGC CTCCAGTTCT 
GAATCAGAGC GACCCATCAC TATCATGGCT TCAGGGAGCA CAGGTGGTGA AAATGATGGC 
CTTCGGAATA GCACTGGACT TGGATCCCAA AACAAGTTTG TGGTTGGTAG CAGCAGCAAT 
AATGTGGGCC ACGGAAGTAG CACTGGGCCG TGGGGTTTTT CCCATGGAGC CATAATAAGC 
ACATGTCAGG CCTCTGTGGA TGCTCCTGAA AGCAAATCAG AAAGTAGCAA CAATAGAATG 
AATGCTTGGG GCACTGTAAG TTCTTCATCA AATGGAGGGT TAAATCCAAG CACTTTGAAT 
TCAGCTAGCA ACCATGGTGC CTGGCCAGTA TTAGAGAACA ACGGACTTGC CCTAAAAGGG 
CCTGTAGGGA GTGGTAGTTC TGGCATCAAT ATTCAGTGCA GTCCCTTAGG CCAGATGCCT 
AACAATCAGA GTATTAACTC TAAAGTGGGT GGTGCTTCCA CCCATGGTAC CTGGGGAAGC 
CTTCAGGAAA CTTGTGAATC TGAAGTAAGT GGTACACAGA AGGTTTCATT CAGTGGTCAA 
CCTCAAAATA TTACCACTGA AATGACTGGA CCAAATAACA CTACTAACTT TATGACCTCT 
AGTTTACCAA ACTCCGGTTC AGTACAGAAT AATGAACTGC CTAGTAATAC AGGGGCCTGG 
CGTGTGAGCA CAATGAATCA TCCTCAGATA CAGGCTCCCT CGGTTGTAAA TGGCACTTCC 
CTTTCTCACC TTAGCAATGG AGAGTCAAAA AGTGGAGGCT CTTACGGTAC TACATGGGGT 
GCCTATGGTT CTAATTACTC TGGAGACAAA TGTTCAAGCC CTAATGGCCA AGCTAATGGT 
GACACTGTGA ATGCAACTCT AATGCAGCCT GGCGTAAACG GGCCTATGGG CACTAACTTT 
CAAGTTAACA CAAACAAAGG AGGCGGCGTG TGGGAGTCTG GAGCAGTGAA TTCCCAGAGT 
GCATCCTGGG GAAATGGTGC AAATTCTGGA GGAAGTCGAA GAGGGTGGGG AACTCCTGCA 
CAAAACACTG GCACTAATAC ACCCGGCGTG GAGTGGAACA AACTGCCTGG CAACCAGCAT 
TCCAGTGACA GTGCAAATGG CAACGGTAAG AAGTTTACAA ACGGATGGAA GTCTACTGAG 
GAAGAGGATC AGGGTTCTGC CGCATCTCAG ACAAATGAGC AAAGCAGTGT GTGGGCCAAA 
ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT ACAGAAAGCA CTGGACGCCT CGAGGAGAAA 
GCGACTGGGG AAAATCAGAG TAGGGACAGA AGGAAGATGG ATCAGCACAC GTTACTCCAA 
AGCATTGTAA ACAGAACTGA CTTAGATCCA CGTGTCCTGT CCAACTCTGG CTGGGGACAG 
ACTCCTATTA AGCAGAATAC TGCCTGGGAT ACAGAAACAT CACCAAGAGG GGAGAGAAAG 
ACTGACAATG GGACAGAGGC CTGGGGAAGC TCTGCAACAC AGACTTTTAA CTCAGGGGCG 
TGTGTAGATA AGACTAGCCC GAGTAGTAAT GATACCTCAT CTGTATCGGG GTGGGGAGAT 
CCCAAACCTG CTCTGCGGTG GGGAGATTCC AAAGGCTCAA GCTGCCAGGG GGGATGGGAA 
GATGACTCTG CTGCTACAGG AATGGTCAAG AGCAATCAGT GGGGGAACTG CAAAGAAGAG 
AAGTCCGCAT GGAATGATTC GCAAAAGAAC AAACAGGGAT GGGGTGACGG ACAAAAGTCA 
AACCAAGGGT GGTCCGTCTC TGCCGGTGAC AACTGGGGAG AAAGGAGTAA CCATTGGGGA 
GAGGCTAACA AGAAGTCTAG CTCAGGAGGT AGTGACAGTG ATAGGTCTGT TTCTGGTTGG 
AATGAACTCG GTAAAACCAG TTCTTTTACA TGGGGAAATA ACATTAATCC AAATAATTCA 
TCAGGCTGGG ACGAATCTTC TAAACCGAAT CCTTCCCAGG GATGGGGAGA CCCTCCAAAG 
TCTAATCAGT CTCTAGGTTG GGGAGATTCA TCAAAGCCGG TCAGTTCTCC AGACTGGAAC 
AAGCAACAAG ACATTGTCGG ATCTTGGGGC ATCCCGCCAG CGACAGGCAA ACCTCCTGGT 
ACAGGCTGGC TGGGAGGACC TATGCCAGCC CCAGCGAAAG AGGAAGAACC CACGGGCTGG 
GAGGAGCCAT CCCCAGAATC CATACGTCGC AAAATGGAGA TTGACGATGG GACTTCAGCT 
TGGGGAGATC CCAGCAAATA CAATTACAAA AATGTGAACA TGTGGAACAA AAACGTCCCA 
AATGGCAGCA GTCGCTCAGA CCAGCAAGCA CAGGTACACC AGTTGCTACC GTCTGCAGGT 
ACCATCTCAA ACAAAGAGGC GAGCAGTGGC TCCGGCTGGG GTGAGCCCTG GGGGGAGACT 
TCAACTCCAG CCACAACTGT GGATAATGGT ACTTCAGCCT GGGGTAAACC CGTAGACAGT 
GGGCCTAGCT GGGGCGAACC CATTGCTGCG GCATCCAGCA CATCCACGTG GGGCTCCAGC 
TCTGTTGGTC CACAAACATT AAACAAATCT GGGCCAAAAT CTATGCAAGA CGGCTGGTGT 
GGTGATGATA TGCCCTTGCC TGGAAATCGC CCCACTGGCT GGGAAGAGGA AGAAGATGTA 
GAGATTGGAA TGTGGAATAG TAATTCATCT CAAGAGCTTA ATTCATCTTT AAATTGGCCA 
CCATATACAA AGAAAATGTC TTCGAAGGGT CTGAGTGGCA AAAAAAGGAG AAGGGAAAGG 
GGAATGATGA AAGGTGGAAA CAAACAAGAA GAAGCATGGA TAAATCCATT TGTTAAACAG 
TTTTCAAATA TCAGTTTTTC GAGAGACTCG CCAGAAGAAA ATGTACAGAG CAATAAGATG 
GACCTTTCTG GAGGAATGTT ACAAGACAAA CGAATGGAGA TAGATAAACA TAGCCTAAAT 
ATTGGTGATT ACAATCGAAC GGTCGGGAAA GGCCCTGGTT CTCGGCCTCA GATTTCCAAA 
GAGTCTTCCA TGGACCGCAG TCCTTATTTT GATAAGAATG GCAATCCCAG TATGTTTGGT 
GTTGGAAACA CAGCAGCACA ACCCCGGGGC ATGCAGCAGC CTCCAGCACA ACCTCTCAGT 
TCATCTCAGC CTAGTCTCCG TGCTCAAGTG CCTCCTCCAT TACTCTCCCC TCAGGTTCCA 
GTTTCATTGC TGAAGTATGC ACCAAACAAC GGTGGCCTGA ATCCGCTCTT TGGCCCTCAA 
CAGGTAGCCA TGCTGAACCA GCTATCCCAA CTAAACCAGC TTTCTCAGAT CTCCCAGTTA 
CAGCGATTGT TAGCGCAGCA GCAAAGGGCG CAGAGTCAGA GAAGCGTGCC TTCTGGGAAC 
CGGCAGCAGC AAGACCAGCA GGGTCGACCT CTTAGCGTGC AGCAGCAGAT GATGCAACAA 
TCTCGTCAAC TTGATCCAAA CCTGTTGGTG AAGCAACAAA CTCCGCCATC TCAGCAACAG 
TCACTCCATC AGCCAGCCAT GAAGTCTTTC CTTGAAAATG TCATGCCCCA CACTACACCT 
GAGCTGCAAA AAGGGCCATC ACCAATAAAT GCTTTCAGCA ACTTCCCTAT AGGCTTGAAC 
TCAAACTTGA ATGTAAATAT GGATATGAAC AGTATTAAAG AGCCACAGTC AAGACTAAGG 
AAGTGGACTA CAGTGGACAG CATTTCTGTG AACACATCTT TGGATCAAAA CTCCAGCAAA 
CATGGTGCTA TTTCAAGTGG TTTCAGGCTG GAAGAGTCTC CATTTGTTCC CTATGACTTT 
ATGAACAGCA GTACTTCACC AGCCAGTCCT CCAGGTTCAA TAGGAGATGG CTGGCCACGT 
GCCAAATCGC CTAACGGCTC TAGCAGTGTT AATTGGCCAC CAGAATTTCG CCCTGGTGAA 
CCATGGAAAG GTTATCCAAA CATTGACCCT GAAACTGACC CTTACGTCAC TCCTGGCAGT 
GTCATAAACA ATCTTTCAAT TAATACTGTG CGGGAAGTTG ACCACCTCAG GGACAGGAAC 
AGTGGGTCAT CCTCATCCTT GAACACCACG CTGCCTTCAA CTAGTGCCTG GTCATCCATT 
CGTGCCTCCA ACTACAACGC TCCCCTCAGC AGCACAGCAC AAAGCACTGC AGCCAGAAAT 
AGTGATTCCA AATTGACATG GTCCCCTGGT TCAGTTACAA ACACCTCTCT GGCTCATGAG 
CTGTGGAAGG TCCCTCTGCC ACCTAAAAAC ATCACTGCTC CGTCCCGCCC ACCTCCGGGA 
CTGACTGGTC AGAAGCCACC CTTGTCTTCG TGGGATAATT CTCCCCTTCG AGTAGGTGGA 
GGATGGGGAA ATTCTGACGC CAGGTATACC CCAGGTTCCA GCTGGGGTGA GAGCAGCTCA 
GGGAGAATAA CAAATTGGCT TGTTCTAAAA AACCTTACAC CTCAGATTGA TGGCTCAACT 
CTGCGTACTC TGTGCATGCA GCATGGCCCA CTGATAACAT TCCACCTGAA CCTCCCACAT 
GGAAACGCTT TGGTCCGTTA CAGTTCAAAA GAAGAGGTAG TGAAGGCACA AAAGTCTCTG 
CACATGTGTG TACTGGGGAA CACTACTATT CTTGCTGAGT TTGCCAGTGA AGAGGAGATC 
AGTCGTTTCT TTGCACAAAG TCAGTCTCTG ACCCCTTCTC CCAGCTGGCA GTCTCTCGGG 
TCCAGCCAGA GCCGGCTGGG CTCCCTCGAC TGTTCCCACT CATTCTCCAG CCGGACCGAT 
CTCAATCACT GGAATGGTGC TGGGCTGTCG GGAACTAACT GTGGAGACCT TCATGGCACT 
TCCCTCTGGG GGACCCCACA TTATTCCACA AGCCTGTGGG GTCCTCCCAG CAGCAGCGAC 
CCCCGGGGAA TTAGCAGCCC ATCCCCCATT AACGCTTTTC TTTCTGTTGA CCACCTGGGT 
GGGGGTGGAG AGTCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840969.1
CDS	493..5793
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985201.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985201.1

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGATACAAA CCACAATACT 
CCAGGATCCC ATTATGAAAA TTCAGAGCGG GGACCTGTGT CGTCTACAAG TGACTCTCGT 
ACAAACTGTA AGAATGCTGT TGTGAACGAC TCCCCTGAAA AAGAAGCATG GCCCTCAGCC 
CCTGGCAGTG ATCCGGAGTT GGTTTCAGAA TGTATGGATG CTGATTCTGC CTCCAGTTCT 
GAATCAGAGC GACCCATCAC TATCATGGCT TCAGGGAGCA CAGGTGGTGA AAATGATGGC 
CTTCGGAATA GCACTGGACT TGGATCCCAA AACAAGTTTG TGGTTGGTAG CAGCAGCAAT 
AATGTGGGCC ACGGAAGTAG CACTGGGCCG TGGGGTTTTT CCCATGGAGC CATAATAAGC 
ACATGTCAGG CCTCTGTGGA TGCTCCTGAA AGCAAATCAG AAAGTAGCAA CAATAGAATG 
AATGCTTGGG GCACTGTAAG TTCTTCATCA AATGGAGGGT TAAATCCAAG CACTTTGAAT 
TCAGCTAGCA ACCATGGTGC CTGGCCAGTA TTAGAGAACA ACGGACTTGC CCTAAAAGGG 
CCTGTAGGGA GTGGTAGTTC TGGCATCAAT ATTCAGTGCA GTCCCTTAGG CCAGATGCCT 
AACAATCAGA GTATTAACTC TAAAGTGGGT GGTGCTTCCA CCCATGGTAC CTGGGGAAGC 
CTTCAGGAAA CTTGTGAATC TGAAGTAAGT GGTACACAGA AGGTTTCATT CAGTGGTCAA 
CCTCAAAATA TTACCACTGA AATGACTGGA CCAAATAACA CTACTAACTT TATGACCTCT 
AGTTTACCAA ACTCCGGTTC AGTACAGAAT AATGAACTGC CTAGTAATAC AGGGGCCTGG 
CGTGTGAGCA CAATGAATCA TCCTCAGATA CAGGCTCCCT CGGTTGTAAA TGGCACTTCC 
CTTTCTCACC TTAGCAATGG AGAGTCAAAA AGTGGAGGCT CTTACGGTAC TACATGGGGT 
GCCTATGGTT CTAATTACTC TGGAGACAAA TGTTCAAGCC CTAATGGCCA AGCTAATGGT 
GACACTGTGA ATGCAACTCT AATGCAGCCT GGCGTAAACG GGCCTATGGG CACTAACTTT 
CAAGTTAACA CAAACAAAGG AGGCGGCGTG TGGGAGTCTG GAGCAGTGAA TTCCCAGAGT 
GCATCCTGGG GAAATGGTGC AAATTCTGGA GGAAGTCGAA GAGGGTGGGG AACTCCTGCA 
CAAAACACTG GCACTAATAC ACCCGGCGTG GAGTGGAACA AACTGCCTGG CAACCAGCAT 
TCCAGTGACA GTGCAAATGG CAACGGTAAG AAGTTTACAA ACGGATGGAA GTCTACTGAG 
GAAGAGGATC AGGGTTCTGC CGCATCTCAG ACAAATGAGC AAAGCAGTGT GTGGGCCAAA 
ACAGGAGGTA CAGTGGAGAG TGAAGGTAGT ACAGAAAGCA CTGGACGCCT CGAGGAGAAA 
GCGACTGGGG AAAATCAGAG TAGGGACAGA AGGAAGATGG ATCAGCACAC GTTACTCCAA 
AGCATTGTAA ACAGAACTGA CTTAGATCCA CGTGTCCTGT CCAACTCTGG CTGGGGACAG 
ACTCCTATTA AGCAGAATAC TGCCTGGGAT ACAGAAACAT CACCAAGAGG GGAGAGAAAG 
ACTGACAATG GGACAGAGGC CTGGGGAAGC TCTGCAACAC AGACTTTTAA CTCAGGGGCG 
TGTGTAGATA AGACTAGCCC GAGTAGTAAT GATACCTCAT CTGTATCGGG GTGGGGAGAT 
CCCAAACCTG CTCTGCGGTG GGGAGATTCC AAAGGCTCAA GCTGCCAGGG GGGATGGGAA 
GATGACTCTG CTGCTACAGG AATGGTCAAG AGCAATCAGT GGGGGAACTG CAAAGAAGAG 
AAGTCCGCAT GGAATGATTC GCAAAAGAAC AAACAGGGAT GGGGTGACGG ACAAAAGTCA 
AACCAAGGGT GGTCCGTCTC TGCCGGTGAC AACTGGGGAG AAAGGAGTAA CCATTGGGGA 
GAGGCTAACA AGAAGTCTAG CTCAGGAGGT AGTGACAGTG ATAGGTCTGT TTCTGGTTGG 
AATGAACTCG GTAAAACCAG TTCTTTTACA TGGGGAAATA ACATTAATCC AAATAATTCA 
TCAGGCTGGG ACGAATCTTC TAAACCGAAT CCTTCCCAGG GATGGGGAGA CCCTCCAAAG 
TCTAATCAGT CTCTAGGTTG GGGAGATTCA TCAAAGCCGG TCAGTTCTCC AGACTGGAAC 
AAGCAACAAG ACATTGTCGG ATCTTGGGGC ATCCCGCCAG CGACAGGCAA ACCTCCTGGT 
ACAGGCTGGC TGGGAGGACC TATGCCAGCC CCAGCGAAAG AGGAAGAACC CACGGGCTGG 
GAGGAGCCAT CCCCAGAATC CATACGTCGC AAAATGGAGA TTGACGATGG GACTTCAGCT 
TGGGGAGATC CCAGCAAATA CAATTACAAA AATGTGAACA TGTGGAACAA AAACGTCCCA 
AATGGCAGCA GTCGCTCAGA CCAGCAAGCA CAGGTACACC AGTTGCTACC GTCTGCAGGT 
ACCATCTCAA ACAAAGAGGC GAGCAGTGGC TCCGGCTGGG GTGAGCCCTG GGGGGAGACT 
TCAACTCCAG CCACAACTGT GGATAATGGT ACTTCAGCCT GGGGTAAACC CGTAGACAGT 
GGGCCTAGCT GGGGCGAACC CATTGCTGCG GCATCCAGCA CATCCACGTG GGGCTCCAGC 
TCTGTTGGTC CACAAACATT AAACAAATCT GGGCCAAAAT CTATGCAAGA CGGCTGGTGT 
GGTGATGATA TGCCCTTGCC TGGAAATCGC CCCACTGGCT GGGAAGAGGA AGAAGATGTA 
GAGATTGGAA TGTGGAATAG TAATTCATCT CAAGAGCTTA ATTCATCTTT AAATTGGCCA 
CCATATACAA AGAAAATGTC TTCGAAGGGT CTGAGTGGCA AAAAAAGGAG AAGGGAAAGG 
GGAATGATGA AAGGTGGAAA CAAACAAGAA GAAGCATGGA TAAATCCATT TGTTAAACAG 
TTTTCAAATA TCAGTTTTTC GAGAGACTCG CCAGAAGAAA ATGTACAGAG CAATAAGATG 
GACCTTTCTG GAGGAATGTT ACAAGACAAA CGAATGGAGA TAGATAAACA TAGCCTAAAT 
ATTGGTGATT ACAATCGAAC GGTCGGGAAA GGCCCTGGTT CTCGGCCTCA GATTTCCAAA 
GAGTCTTCCA TGGACCGCAG TCCTTATTTT GATAAGAATG GCAATCCCAG TATGTTTGGT 
GTTGGAAACA CAGCAGCACA ACCCCGGGGC ATGCAGCAGC CTCCAGCACA ACCTCTCAGT 
TCATCTCAGC CTAGTCTCCG TGCTCAAGTG CCTCCTCCAT TACTCTCCCC TCAGGTTCCA 
GTTTCATTGC TGAAGTATGC ACCAAACAAC GGTGGCCTGA ATCCGCTCTT TGGCCCTCAA 
CAGGTAGCCA TGCTGAACCA GCTATCCCAA CTAAACCAGC TTTCTCAGAT CTCCCAGTTA 
CAGCGATTGT TAGCGCAGCA GCAAAGGGCG CAGAGTCAGA GAAGCGTGCC TTCTGGGAAC 
CGGCAGCAGC AAGACCAGCA GGGTCGACCT CTTAGCGTGC AGCAGCAGAT GATGCAACAA 
TCTCGTCAAC TTGATCCAAA CCTGTTGGTG AAGCAACAAA CTCCGCCATC TCAGCAACAG 
TCACTCCATC AGCCAGCCAT GAAGTCTTTC CTTGAAAATG TCATGCCCCA CACTACACCT 
GAGCTGCAAA AAGGGCCATC ACCAATAAAT GCTTTCAGCA ACTTCCCTAT AGGCTTGAAC 
TCAAACTTGA ATGTAAATAT GGATATGAAC AGTATTAAAG AGCCACAGTC AAGACTAAGG 
AAGTGGACTA CAGTGGACAG CATTTCTGTG AACACATCTT TGGATCAAAA CTCCAGCAAA 
CATGGTGCTA TTTCAAGTGG TTTCAGGCTG GAAGAGTCTC CATTTGTTCC CTATGACTTT 
ATGAACAGCA GTACTTCACC AGCCAGTCCT CCAGGTTCAA TAGGAGATGG CTGGCCACGT 
GCCAAATCGC CTAACGGCTC TAGCAGTGTT AATTGGCCAC CAGAATTTCG CCCTGGTGAA 
CCATGGAAAG GTTATCCAAA CATTGACCCT GAAACTGACC CTTACGTCAC TCCTGGCAGT 
GTCATAAACA ATCTTTCAAT TAATACTGTG CGGGAAGTTG ACCACCTCAG GGACAGGAAC 
AGTGGGTCAT CCTCATCCTT GAACACCACG CTGCCTTCAA CTAGTGCCTG GTCATCCATT 
CGTGCCTCCA ACTACAACGC TCCCCTCAGC AGCACAGCAC AAAGCACTGC AGCCAGAAAT 
AGTGATTCCA AATTGACATG GTCCCCTGGT TCAGTTACAA ACACCTCTCT GGCTCATGAG 
CTGTGGAAGG TCCCTCTGCC ACCTAAAAAC ATCACTGCTC CGTCCCGCCC ACCTCCGGGA 
CTGACTGGTC AGAAGCCACC CTTGTCTTCG TGGGATAATT CTCCCCTTCG AGTAGGTGGA 
GGATGGGGAA ATTCTGACGC CAGGTATACC CCAGGTTCCA GCTGGGGTGA GAGCAGCTCA 
GGGAGAATAA CAAATTGGCT TGTTCTAAAA AACCTTACAC CTCAGATTGA TGGCTCAACT 
CTGCGTACTC TGTGCATGCA GCATGGCCCA CTGATAACAT TCCACCTGAA CCTCCCACAT 
GGAAACGCTT TGGTCCGTTA CAGTTCAAAA GAAGAGGTAG TGAAGGCACA AAAGTCTCTG 
CACATGTGTG TACTGGGGAA CACTACTATT CTTGCTGAGT TTGCCAGTGA AGAGGAGATC 
AGTCGTTTCT TTGCACAAAG TCAGTCTCTG ACCCCTTCTC CCAGCTGGCA GTCTCTCGGG 
TCCAGCCAGA GCCGGCTGGG CTCCCTCGAC TGTTCCCACT CATTCTCCAG CCGGACCGAT 
CTCAATCACT GGAATGGTGC TGGGCTGTCG GGAACTAACT GTGGAGACCT TCATGGCACT 
TCCCTCTGGG GGACCCCACA TTATTCCACA AGCCTGTGGG GTCCTCCCAG CAGCAGCGAC 
CCCCGGGGAA TTAGCAGCCC ATCCCCCATT AACGCTTTTC TTTCTGTTGA CCACCTGGGT 
GGGGGTGGAG AGTCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985202.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840970.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OBa230530
Clone ID Related Accession (Same CDS sequence)	XM_024985202.1
Accession Version	XM_024985202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTGCT TTGGCAGCCT ACTGGATGTG ATAGATACAA ACCACAATAC TCCAGGATCC 
CATTATGAAA ATTCAGAGCG GGGACCTGTG TCGTCTACAA GTGACTCTCG TACAAACTGT 
AAGAATGCTG TTGTGAACGA CTCCCCTGAA AAAGAAGCAT GGCCCTCAGC CCCTGGCAGT 
GATCCGGAGT TGGTTTCAGA ATGTATGGAT GCTGATTCTG CCTCCAGTTC TGAATCAGAG 
CGACCCATCA CTATCATGGC TTCAGGGAGC ACAGGTGGTG AAAATGATGG CCTTCGGAAT 
AGCACTGGAC TTGGATCCCA AAACAAGTTT GTGGTTGGTA GCAGCAGCAA TAATGTGGGC 
CACGGAAGTA GCACTGGGCC GTGGGGTTTT TCCCATGGAG CCATAATAAG CACATGTCAG 
GCCTCTGTGG ATGCTCCTGA AAGCAAATCA GAAAGTAGCA ACAATAGAAT GAATGCTTGG 
GGCACTGTAA GTTCTTCATC AAATGGAGGG TTAAATCCAA GCACTTTGAA TTCAGCTAGC 
AACCATGGTG CCTGGCCAGT ATTAGAGAAC AACGGACTTG CCCTAAAAGG GCCTGTAGGG 
AGTGGTAGTT CTGGCATCAA TATTCAGTGC AGTCCCTTAG GCCAGATGCC TAACAATCAG 
AGTATTAACT CTAAAGTGGG TGGTGCTTCC ACCCATGGTA CCTGGGGAAG CCTTCAGGAA 
ACTTGTGAAT CTGAAGTAAG TGGTACACAG AAGGTTTCAT TCAGTGGTCA ACCTCAAAAT 
ATTACCACTG AAATGACTGG ACCAAATAAC ACTACTAACT TTATGACCTC TAGTTTACCA 
AACTCCGGTT CAGTACAGAA TAATGAACTG CCTAGTAATA CAGGGGCCTG GCGTGTGAGC 
ACAATGAATC ATCCTCAGAT ACAGGCTCCC TCGGTTGTAA ATGGCACTTC CCTTTCTCAC 
CTTAGCAATG GAGAGTCAAA AAGTGGAGGC TCTTACGGTA CTACATGGGG TGCCTATGGT 
TCTAATTACT CTGGAGACAA ATGTTCAAGC CCTAATGGCC AAGCTAATGG TGACACTGTG 
AATGCAACTC TAATGCAGCC TGGCGTAAAC GGGCCTATGG GCACTAACTT TCAAGTTAAC 
ACAAACAAAG GAGGCGGCGT GTGGGAGTCT GGAGCAGTGA ATTCCCAGAG TGCATCCTGG 
GGAAATGGTG CAAATTCTGG AGGAAGTCGA AGAGGGTGGG GAACTCCTGC ACAAAACACT 
GGCACTAATA CACCCGGCGT GGAGTGGAAC AAACTGCCTG GCAACCAGCA TTCCAGTGAC 
AGTGCAAATG GCAACGGTAA GAAGTTTACA AACGGATGGA AGTCTACTGA GGAAGAGGAT 
CAGGGTTCTG CCGCATCTCA GACAAATGAG CAAAGCAGTG TGTGGGCCAA AACAGGAGGT 
ACAGTGGAGA GTGAAGGTAG TACAGAAAGC ACTGGACGCC TCGAGGAGAA AGCGACTGGG 
GAAAATCAGA GTAGGGACAG AAGGAAGATG GATCAGCACA CGTTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTG TCCAACTCTG GCTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAAACA TCACCAAGAG GGGAGAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC GTGTGTAGAT 
AAGACTAGCC CGAGTAGTAA TGATACCTCA TCTGTATCGG GGTGGGGAGA TCCCAAACCT 
GCTCTGCGGT GGGGAGATTC CAAAGGCTCA AGCTGCCAGG GGGGATGGGA AGATGACTCT 
GCTGCTACAG GAATGGTCAA GAGCAATCAG TGGGGGAACT GCAAAGAAGA GAAGTCCGCA 
TGGAATGATT CGCAAAAGAA CAAACAGGGA TGGGGTGACG GACAAAAGTC AAACCAAGGG 
TGGTCCGTCT CTGCCGGTGA CAACTGGGGA GAAAGGAGTA ACCATTGGGG AGAGGCTAAC 
AAGAAGTCTA GCTCAGGAGG TAGTGACAGT GATAGGTCTG TTTCTGGTTG GAATGAACTC 
GGTAAAACCA GTTCTTTTAC ATGGGGAAAT AACATTAATC CAAATAATTC ATCAGGCTGG 
GACGAATCTT CTAAACCGAA TCCTTCCCAG GGATGGGGAG ACCCTCCAAA GTCTAATCAG 
TCTCTAGGTT GGGGAGATTC ATCAAAGCCG GTCAGTTCTC CAGACTGGAA CAAGCAACAA 
GACATTGTCG GATCTTGGGG CATCCCGCCA GCGACAGGCA AACCTCCTGG TACAGGCTGG 
CTGGGAGGAC CTATGCCAGC CCCAGCGAAA GAGGAAGAAC CCACGGGCTG GGAGGAGCCA 
TCCCCAGAAT CCATACGTCG CAAAATGGAG ATTGACGATG GGACTTCAGC TTGGGGAGAT 
CCCAGCAAAT ACAATTACAA AAATGTGAAC ATGTGGAACA AAAACGTCCC AAATGGCAGC 
AGTCGCTCAG ACCAGCAAGC ACAGGTACAC CAGTTGCTAC CGTCTGCAGG TACCATCTCA 
AACAAAGAGG CGAGCAGTGG CTCCGGCTGG GGTGAGCCCT GGGGGGAGAC TTCAACTCCA 
GCCACAACTG TGGATAATGG TACTTCAGCC TGGGGTAAAC CCGTAGACAG TGGGCCTAGC 
TGGGGCGAAC CCATTGCTGC GGCATCCAGC ACATCCACGT GGGGCTCCAG CTCTGTTGGT 
CCACAAACAT TAAACAAATC TGGGCCAAAA TCTATGCAAG ACGGCTGGTG TGGTGATGAT 
ATGCCCTTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAAGATGT AGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AATTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CTTCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCATGG ATAAATCCAT TTGTTAAACA GTTTTCAAAT 
ATCAGTTTTT CGAGAGACTC GCCAGAAGAA AATGTACAGA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGACCGCA GTCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTCAG TTCATCTCAG 
CCTAGTCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAACAA ACTCCGCCAT CTCAGCAACA GTCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGAAAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG CTCCCCTCAG CAGCACAGCA CAAAGCACTG CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCCCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTCTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTTC GTGGGATAAT TCTCCCCTTC GAGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATTG ATGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAACGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCAGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCATGGCAC TTCCCTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840970.1
CDS	16..5187
Translation	MPCFGSLLDVIDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985202.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985202.1

ORF Insert Sequence:

ATGCCGTGCT TTGGCAGCCT ACTGGATGTG ATAGATACAA ACCACAATAC TCCAGGATCC 
CATTATGAAA ATTCAGAGCG GGGACCTGTG TCGTCTACAA GTGACTCTCG TACAAACTGT 
AAGAATGCTG TTGTGAACGA CTCCCCTGAA AAAGAAGCAT GGCCCTCAGC CCCTGGCAGT 
GATCCGGAGT TGGTTTCAGA ATGTATGGAT GCTGATTCTG CCTCCAGTTC TGAATCAGAG 
CGACCCATCA CTATCATGGC TTCAGGGAGC ACAGGTGGTG AAAATGATGG CCTTCGGAAT 
AGCACTGGAC TTGGATCCCA AAACAAGTTT GTGGTTGGTA GCAGCAGCAA TAATGTGGGC 
CACGGAAGTA GCACTGGGCC GTGGGGTTTT TCCCATGGAG CCATAATAAG CACATGTCAG 
GCCTCTGTGG ATGCTCCTGA AAGCAAATCA GAAAGTAGCA ACAATAGAAT GAATGCTTGG 
GGCACTGTAA GTTCTTCATC AAATGGAGGG TTAAATCCAA GCACTTTGAA TTCAGCTAGC 
AACCATGGTG CCTGGCCAGT ATTAGAGAAC AACGGACTTG CCCTAAAAGG GCCTGTAGGG 
AGTGGTAGTT CTGGCATCAA TATTCAGTGC AGTCCCTTAG GCCAGATGCC TAACAATCAG 
AGTATTAACT CTAAAGTGGG TGGTGCTTCC ACCCATGGTA CCTGGGGAAG CCTTCAGGAA 
ACTTGTGAAT CTGAAGTAAG TGGTACACAG AAGGTTTCAT TCAGTGGTCA ACCTCAAAAT 
ATTACCACTG AAATGACTGG ACCAAATAAC ACTACTAACT TTATGACCTC TAGTTTACCA 
AACTCCGGTT CAGTACAGAA TAATGAACTG CCTAGTAATA CAGGGGCCTG GCGTGTGAGC 
ACAATGAATC ATCCTCAGAT ACAGGCTCCC TCGGTTGTAA ATGGCACTTC CCTTTCTCAC 
CTTAGCAATG GAGAGTCAAA AAGTGGAGGC TCTTACGGTA CTACATGGGG TGCCTATGGT 
TCTAATTACT CTGGAGACAA ATGTTCAAGC CCTAATGGCC AAGCTAATGG TGACACTGTG 
AATGCAACTC TAATGCAGCC TGGCGTAAAC GGGCCTATGG GCACTAACTT TCAAGTTAAC 
ACAAACAAAG GAGGCGGCGT GTGGGAGTCT GGAGCAGTGA ATTCCCAGAG TGCATCCTGG 
GGAAATGGTG CAAATTCTGG AGGAAGTCGA AGAGGGTGGG GAACTCCTGC ACAAAACACT 
GGCACTAATA CACCCGGCGT GGAGTGGAAC AAACTGCCTG GCAACCAGCA TTCCAGTGAC 
AGTGCAAATG GCAACGGTAA GAAGTTTACA AACGGATGGA AGTCTACTGA GGAAGAGGAT 
CAGGGTTCTG CCGCATCTCA GACAAATGAG CAAAGCAGTG TGTGGGCCAA AACAGGAGGT 
ACAGTGGAGA GTGAAGGTAG TACAGAAAGC ACTGGACGCC TCGAGGAGAA AGCGACTGGG 
GAAAATCAGA GTAGGGACAG AAGGAAGATG GATCAGCACA CGTTACTCCA AAGCATTGTA 
AACAGAACTG ACTTAGATCC ACGTGTCCTG TCCAACTCTG GCTGGGGACA GACTCCTATT 
AAGCAGAATA CTGCCTGGGA TACAGAAACA TCACCAAGAG GGGAGAGAAA GACTGACAAT 
GGGACAGAGG CCTGGGGAAG CTCTGCAACA CAGACTTTTA ACTCAGGGGC GTGTGTAGAT 
AAGACTAGCC CGAGTAGTAA TGATACCTCA TCTGTATCGG GGTGGGGAGA TCCCAAACCT 
GCTCTGCGGT GGGGAGATTC CAAAGGCTCA AGCTGCCAGG GGGGATGGGA AGATGACTCT 
GCTGCTACAG GAATGGTCAA GAGCAATCAG TGGGGGAACT GCAAAGAAGA GAAGTCCGCA 
TGGAATGATT CGCAAAAGAA CAAACAGGGA TGGGGTGACG GACAAAAGTC AAACCAAGGG 
TGGTCCGTCT CTGCCGGTGA CAACTGGGGA GAAAGGAGTA ACCATTGGGG AGAGGCTAAC 
AAGAAGTCTA GCTCAGGAGG TAGTGACAGT GATAGGTCTG TTTCTGGTTG GAATGAACTC 
GGTAAAACCA GTTCTTTTAC ATGGGGAAAT AACATTAATC CAAATAATTC ATCAGGCTGG 
GACGAATCTT CTAAACCGAA TCCTTCCCAG GGATGGGGAG ACCCTCCAAA GTCTAATCAG 
TCTCTAGGTT GGGGAGATTC ATCAAAGCCG GTCAGTTCTC CAGACTGGAA CAAGCAACAA 
GACATTGTCG GATCTTGGGG CATCCCGCCA GCGACAGGCA AACCTCCTGG TACAGGCTGG 
CTGGGAGGAC CTATGCCAGC CCCAGCGAAA GAGGAAGAAC CCACGGGCTG GGAGGAGCCA 
TCCCCAGAAT CCATACGTCG CAAAATGGAG ATTGACGATG GGACTTCAGC TTGGGGAGAT 
CCCAGCAAAT ACAATTACAA AAATGTGAAC ATGTGGAACA AAAACGTCCC AAATGGCAGC 
AGTCGCTCAG ACCAGCAAGC ACAGGTACAC CAGTTGCTAC CGTCTGCAGG TACCATCTCA 
AACAAAGAGG CGAGCAGTGG CTCCGGCTGG GGTGAGCCCT GGGGGGAGAC TTCAACTCCA 
GCCACAACTG TGGATAATGG TACTTCAGCC TGGGGTAAAC CCGTAGACAG TGGGCCTAGC 
TGGGGCGAAC CCATTGCTGC GGCATCCAGC ACATCCACGT GGGGCTCCAG CTCTGTTGGT 
CCACAAACAT TAAACAAATC TGGGCCAAAA TCTATGCAAG ACGGCTGGTG TGGTGATGAT 
ATGCCCTTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAAGATGT AGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AATTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CTTCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCATGG ATAAATCCAT TTGTTAAACA GTTTTCAAAT 
ATCAGTTTTT CGAGAGACTC GCCAGAAGAA AATGTACAGA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGACCGCA GTCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTCAG TTCATCTCAG 
CCTAGTCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCGCTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA ACTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCAGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGCGTG CAGCAGCAGA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAACAA ACTCCGCCAT CTCAGCAACA GTCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGAAAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACT 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GCCCTGGTGA ACCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG CTCCCCTCAG CAGCACAGCA CAAAGCACTG CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCCCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTCTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTTC GTGGGATAAT TCTCCCCTTC GAGTAGGTGG AGGATGGGGA 
AATTCTGACG CCAGGTATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATTG ATGGCTCAAC TCTGCGTACT 
CTGTGCATGC AGCATGGCCC ACTGATAACA TTCCACCTGA ACCTCCCACA TGGAAACGCT 
TTGGTCCGTT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GTCAGTCTCT GACCCCTTCT CCCAGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCATGGCAC TTCCCTCTGG 
GGGACCCCAC ATTATTCCAC AAGCCTGTGG GGTCCTCCCA GCAGCAGCGA CCCCCGGGGA 
ATTAGCAGCC CATCCCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985203.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840971.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

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CloneID	OBa230531
Clone ID Related Accession (Same CDS sequence)	XM_024985203.1 , XM_024985204.1 , XM_024985205.1
Accession Version	XM_024985203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840971.1
CDS	716..5683
Translation	MDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985203.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985204.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840972.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

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CloneID	OBa230531
Clone ID Related Accession (Same CDS sequence)	XM_024985203.1 , XM_024985204.1 , XM_024985205.1
Accession Version	XM_024985204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840972.1
CDS	784..5751
Translation	MDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985204.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985204.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024985205.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840973.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

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CloneID	OBa230531
Clone ID Related Accession (Same CDS sequence)	XM_024985203.1 , XM_024985204.1 , XM_024985205.1
Accession Version	XM_024985205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840973.1
CDS	763..5730
Translation	MDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024985205.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985205.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGAATGGCA ATCCCAGTAT GTTTGGTGTT GGAAACACAG CAGCACAACC CCGGGGCATG 
CAGCAGCCTC CAGCACAACC TCTCAGTTCA TCTCAGCCTA GTCTCCGTGC TCAAGTGCCT 
CCTCCATTAC TCTCCCCTCA GGTTCCAGTT TCATTGCTGA AGTATGCACC AAACAACGGT 
GGCCTGAATC CGCTCTTTGG CCCTCAACAG GTAGCCATGC TGAACCAGCT ATCCCAACTA 
AACCAGCTTT CTCAGATCTC CCAGTTACAG CGATTGTTAG CGCAGCAGCA AAGGGCGCAG 
AGTCAGAGAA GCGTGCCTTC TGGGAACCGG CAGCAGCAAG ACCAGCAGGG TCGACCTCTT 
AGCGTGCAGC AGCAGATGAT GCAACAATCT CGTCAACTTG ATCCAAACCT GTTGGTGAAG 
CAACAAACTC CGCCATCTCA GCAACAGTCA CTCCATCAGC CAGCCATGAA GTCTTTCCTT 
GAAAATGTCA TGCCCCACAC TACACCTGAG CTGCAAAAAG GGCCATCACC AATAAATGCT 
TTCAGCAACT TCCCTATAGG CTTGAACTCA AACTTGAATG TAAATATGGA TATGAACAGT 
ATTAAAGAGC CACAGTCAAG ACTAAGGAAG TGGACTACAG TGGACAGCAT TTCTGTGAAC 
ACATCTTTGG ATCAAAACTC CAGCAAACAT GGTGCTATTT CAAGTGGTTT CAGGCTGGAA 
GAGTCTCCAT TTGTTCCCTA TGACTTTATG AACAGCAGTA CTTCACCAGC CAGTCCTCCA 
GGTTCAATAG GAGATGGCTG GCCACGTGCC AAATCGCCTA ACGGCTCTAG CAGTGTTAAT 
TGGCCACCAG AATTTCGCCC TGGTGAACCA TGGAAAGGTT ATCCAAACAT TGACCCTGAA 
ACTGACCCTT ACGTCACTCC TGGCAGTGTC ATAAACAATC TTTCAATTAA TACTGTGCGG 
GAAGTTGACC ACCTCAGGGA CAGGAACAGT GGGTCATCCT CATCCTTGAA CACCACGCTG 
CCTTCAACTA GTGCCTGGTC ATCCATTCGT GCCTCCAACT ACAACGCTCC CCTCAGCAGC 
ACAGCACAAA GCACTGCAGC CAGAAATAGT GATTCCAAAT TGACATGGTC CCCTGGTTCA 
GTTACAAACA CCTCTCTGGC TCATGAGCTG TGGAAGGTCC CTCTGCCACC TAAAAACATC 
ACTGCTCCGT CCCGCCCACC TCCGGGACTG ACTGGTCAGA AGCCACCCTT GTCTTCGTGG 
GATAATTCTC CCCTTCGAGT AGGTGGAGGA TGGGGAAATT CTGACGCCAG GTATACCCCA 
GGTTCCAGCT GGGGTGAGAG CAGCTCAGGG AGAATAACAA ATTGGCTTGT TCTAAAAAAC 
CTTACACCTC AGATTGATGG CTCAACTCTG CGTACTCTGT GCATGCAGCA TGGCCCACTG 
ATAACATTCC ACCTGAACCT CCCACATGGA AACGCTTTGG TCCGTTACAG TTCAAAAGAA 
GAGGTAGTGA AGGCACAAAA GTCTCTGCAC ATGTGTGTAC TGGGGAACAC TACTATTCTT 
GCTGAGTTTG CCAGTGAAGA GGAGATCAGT CGTTTCTTTG CACAAAGTCA GTCTCTGACC 
CCTTCTCCCA GCTGGCAGTC TCTCGGGTCC AGCCAGAGCC GGCTGGGCTC CCTCGACTGT 
TCCCACTCAT TCTCCAGCCG GACCGATCTC AATCACTGGA ATGGTGCTGG GCTGTCGGGA 
ACTAACTGTG GAGACCTTCA TGGCACTTCC CTCTGGGGGA CCCCACATTA TTCCACAAGC 
CTGTGGGGTC CTCCCAGCAG CAGCGACCCC CGGGGAATTA GCAGCCCATC CCCCATTAAC 
GCTTTTCTTT CTGTTGACCA CCTGGGTGGG GGTGGAGAGT CCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015460350.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315836.2 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.

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CloneID	OBa230532
Clone ID Related Accession (Same CDS sequence)	XM_015460350.2
Accession Version	XM_015460350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGGATGG CATTGTAGCA GATGAATCCC AAAACATGCA GTTTATGTCC 
AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT AGTGCACTAC CTAACCAGGC CCTGGGCTCC 
ATAGCAGGGC TGGGTATGCA AAACTTGAAT TCTGTTAGAC AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315836.2
CDS	493..6339
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTPGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015460350.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460350.2

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTC CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGGATGG CATTGTAGCA GATGAATCCC AAAACATGCA GTTTATGTCC 
AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT AGTGCACTAC CTAACCAGGC CCTGGGCTCC 
ATAGCAGGGC TGGGTATGCA AAACTTGAAT TCTGTTAGAC AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_010819374.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817676.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

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CloneID	OBa33026
Clone ID Related Accession (Same CDS sequence)	XM_010819374.2
Accession Version	XM_010819374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCAGCA GCAGCAGCAG CAACAGCCGC AACCGCAGCA GCAGCAACCG 
CAGCAGCCGC AGCAGCCGCA GCCGCCACCA CAGGCCTTGC CTCGGTATCC CCGGGAAGTA 
CCACCTCGAT TTCGCCACCA GGAACATAAG CAGCTTCTAA AGAGGGGTCA GCATTTTCCC 
GTCATAGCAG CAAACCTGGG ATCTGCTGTT AAAGTGTTAA GCAGCCAGTC AGAAAGCAGT 
GCTTTAACAA ATCAACAGCC ACAAAATAAC GGAGAGGTGC AGATCAGCAA AAACCAGTCA 
GATACAAACC ACAATACTTC AGGATCCCAT TATGAAAATT CAGAGCGGGG ACCTGTGTCG 
TCTACAAGTG ACTCTCGTAC AAACTGTAAG AATGCTGTTG TGAACGACTC CCCTGAAAAA 
GAAGCATGGC CCTCAGCCCC TGGCAGTGAT CCGGAGTTGG TTTCAGAATG TATGGATGCT 
GATTCTGCCT CCAGTTCTGA ATCAGAGCGA CCCATCACTA TCATGGCTTC AGGGAGCACA 
GGTGGTGAAA ATGATGGCCT TCGGAATAGC ACTGGACTTG GATCCCAAAA CAAGTTTGTG 
GTTGGTAGCA GCAGCAATAA TGTGGGCCAC GGAAGTAGCA CTGGGCCGTG GGGTTTTTCC 
CATGGAGCCA TAATAAGCAC ATGTCAGGCC TCTGTGGATG CTCCTGAAAG CAAATCAGAA 
AGTAGCAACA ATAGAATGAA TGCTTGGGGC ACTGTAAGTT CTTCATCAAA TGGAGGGTTA 
AATCCAAGCA CTTTGAATTC AGCTAGCAAC CATGGTGCCT GGCCAGTATT AGAGAACAAC 
GGACTTGCCC TAAAAGGGCC TGTAGGGAGT GGTAGTTCTG GCATCAATAT TCAGTGCAGT 
CCCTTAGGCC AGATGCCTAA CAATCAGAGT ATTAACTCTA AAGTGGGTGG TGCTTCCACC 
CATGGTACCT GGGGAAGCCT TCAGGAAACT TGTGAATCTG AAGTAAGTGG TACACAGAAG 
GTTTCATTCA GTGGTCAACC TCAAAATATT ACCACTGAAA TGACTGGACC AAATAACACT 
ACTAACTTTA TGACCTCTAG TTTACCAAAC TCCGGTTCAG TACAGAATAA TGAACTGCCT 
AGTAATACAG GGGCCTGGCG TGTGAGCACA ATGAATCATC CTCAGATACA GGCTCCCTCG 
GTTGTAAATG GCACTTCCCT TTCTCACCTT AGCAATGGAG AGTCAAAAAG TGGAGGCTCT 
TACGGTACTA CATGGGGTGC CTATGGTTCT AATTACTCTG GAGACAAATG TTCAAGCCCT 
AATGGCCAAG CTAATGGTGA CACTGTGAAT GCAACTCTAA TGCAGCCTGG CGTAAACGGG 
CCTATGGGCA CTAACTTTCA AGTTAACACA AACAAAGGAG GCGGCGTGTG GGAGTCTGGA 
GCAGTGAATT CCCAGAGTGC ATCCTGGGGA AATGGTGCAA ATTCTGGAGG AAGTCGAAGA 
GGGTGGGGAA CTCCTGCACA AAACACTGGC ACTAATACAC CCGGCGTGGA GTGGAACAAA 
CTGCCTGGCA ACCAGCATTC CAGTGACAGT GCAAATGGCA ACGGTAAGAA GTTTACAAAC 
GGATGGAAGT CTACTGAGGA AGAGGATCAG GGTTCTGCCG CATCTCAGAC AAATGAGCAA 
AGCAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC GACTGGGGAA AATCAGAGTA GGGACAGAAG GAAGATGGAT 
CAGCACACGT TACTCCAAAG CATTGTAAAC AGAACTGACT TAGATCCACG TGTCCTGTCC 
AACTCTGGCT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC AGAAACATCA 
CCAAGAGGGG AGAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCGTG TGTAGATAAG ACTAGCCCGA GTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGCGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGACTCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACTGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAACAA ACAGGGATGG 
GGTGACGGAC AAAAGTCAAA CCAAGGGTGG TCCGTCTCTG CCGGTGACAA CTGGGGAGAA 
AGGAGTAACC ATTGGGGAGA GGCTAACAAG AAGTCTAGCT CAGGAGGTAG TGACAGTGAT 
AGGTCTGTTT CTGGTTGGAA TGAACTCGGT AAAACCAGTT CTTTTACATG GGGAAATAAC 
ATTAATCCAA ATAATTCATC AGGCTGGGAC GAATCTTCTA AACCGAATCC TTCCCAGGGA 
TGGGGAGACC CTCCAAAGTC TAATCAGTCT CTAGGTTGGG GAGATTCATC AAAGCCGGTC 
AGTTCTCCAG ACTGGAACAA GCAACAAGAC ATTGTCGGAT CTTGGGGCAT CCCGCCAGCG 
ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG GGAGGACCTA TGCCAGCCCC AGCGAAAGAG 
GAAGAACCCA CGGGCTGGGA GGAGCCATCC CCAGAATCCA TACGTCGCAA AATGGAGATT 
GACGATGGGA CTTCAGCTTG GGGAGATCCC AGCAAATACA ATTACAAAAA TGTGAACATG 
TGGAACAAAA ACGTCCCAAA TGGCAGCAGT CGCTCAGACC AGCAAGCACA GGTACACCAG 
TTGCTACCGT CTGCAGGTAC CATCTCAAAC AAAGAGGCGA GCAGTGGCTC CGGCTGGGGT 
GAGCCCTGGG GGGAGACTTC AACTCCAGCC ACAACTGTGG ATAATGGTAC TTCAGCCTGG 
GGTAAACCCG TAGACAGTGG GCCTAGCTGG GGCGAACCCA TTGCTGCGGC ATCCAGCACA 
TCCACGTGGG GCTCCAGCTC TGTTGGTCCA CAAACATTAA ACAAATCTGG GCCAAAATCT 
ATGCAAGACG GCTGGTGTGG TGATGATATG CCCTTGCCTG GAAATCGCCC CACTGGCTGG 
GAAGAGGAAG AAGATGTAGA GATTGGAATG TGGAATAGTA ATTCATCTCA AGAGCTTAAT 
TCATCTTTAA ATTGGCCACC ATATACAAAG AAAATGTCTT CGAAGGGTCT GAGTGGCAAA 
AAAAGGAGAA GGGAAAGGGG AATGATGAAA GGTGGAAACA AACAAGAAGA AGCATGGATA 
AATCCATTTG TTAAACAGTT TTCAAATATC AGTTTTTCGA GAGACTCGCC AGAAGAAAAT 
GTACAGAGCA ATAAGATGGA CCTTTCTGGA GGAATGTTAC AAGACAAACG AATGGAGATA 
GATAAACATA GCCTAAATAT TGGTGATTAC AATCGAACGG TCGGGAAAGG CCCTGGTTCT 
CGGCCTCAGA TTTCCAAAGA GTCTTCCATG GACCGCAGTC CTTATTTTGA TAAGGATGGC 
ATTGTAGCAG ATGAATCCCA AAACATGCAG TTTATGTCCA GTCAAAGCAT GAAGCTTCCC 
CCTTCAAATA GTGCACTACC TAACCAGGCC CTGGGCTCCA TAGCAGGGCT GGGTATGCAA 
AACTTGAATT CTGTTAGACA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817676.1
CDS	156..5921
Translation	MEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_010819374.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819374.2

ORF Insert Sequence:

ATGGAAGAAA AGAAAAAGAA AAAAGACGAC AAGAAAAAGA AGGAAGCTGC TCAAAAGAAG 
GCCACTGAAC AAAAAATCAA AGTGCCAGAA CAGATAAAGC CCAGTGTAAG CCAGCCTCAG 
CCTGCCAACT CTAATAACGG CACTTCCACA GCAACCAGCA CTAATAATAA TGCCAAGCGA 
GCCACAGCCA ACAATCAGCA GCAGCAGCAG CAACAGCCGC AACCGCAGCA GCAGCAACCG 
CAGCAGCCGC AGCAGCCGCA GCCGCCACCA CAGGCCTTGC CTCGGTATCC CCGGGAAGTA 
CCACCTCGAT TTCGCCACCA GGAACATAAG CAGCTTCTAA AGAGGGGTCA GCATTTTCCC 
GTCATAGCAG CAAACCTGGG ATCTGCTGTT AAAGTGTTAA GCAGCCAGTC AGAAAGCAGT 
GCTTTAACAA ATCAACAGCC ACAAAATAAC GGAGAGGTGC AGATCAGCAA AAACCAGTCA 
GATACAAACC ACAATACTTC AGGATCCCAT TATGAAAATT CAGAGCGGGG ACCTGTGTCG 
TCTACAAGTG ACTCTCGTAC AAACTGTAAG AATGCTGTTG TGAACGACTC CCCTGAAAAA 
GAAGCATGGC CCTCAGCCCC TGGCAGTGAT CCGGAGTTGG TTTCAGAATG TATGGATGCT 
GATTCTGCCT CCAGTTCTGA ATCAGAGCGA CCCATCACTA TCATGGCTTC AGGGAGCACA 
GGTGGTGAAA ATGATGGCCT TCGGAATAGC ACTGGACTTG GATCCCAAAA CAAGTTTGTG 
GTTGGTAGCA GCAGCAATAA TGTGGGCCAC GGAAGTAGCA CTGGGCCGTG GGGTTTTTCC 
CATGGAGCCA TAATAAGCAC ATGTCAGGCC TCTGTGGATG CTCCTGAAAG CAAATCAGAA 
AGTAGCAACA ATAGAATGAA TGCTTGGGGC ACTGTAAGTT CTTCATCAAA TGGAGGGTTA 
AATCCAAGCA CTTTGAATTC AGCTAGCAAC CATGGTGCCT GGCCAGTATT AGAGAACAAC 
GGACTTGCCC TAAAAGGGCC TGTAGGGAGT GGTAGTTCTG GCATCAATAT TCAGTGCAGT 
CCCTTAGGCC AGATGCCTAA CAATCAGAGT ATTAACTCTA AAGTGGGTGG TGCTTCCACC 
CATGGTACCT GGGGAAGCCT TCAGGAAACT TGTGAATCTG AAGTAAGTGG TACACAGAAG 
GTTTCATTCA GTGGTCAACC TCAAAATATT ACCACTGAAA TGACTGGACC AAATAACACT 
ACTAACTTTA TGACCTCTAG TTTACCAAAC TCCGGTTCAG TACAGAATAA TGAACTGCCT 
AGTAATACAG GGGCCTGGCG TGTGAGCACA ATGAATCATC CTCAGATACA GGCTCCCTCG 
GTTGTAAATG GCACTTCCCT TTCTCACCTT AGCAATGGAG AGTCAAAAAG TGGAGGCTCT 
TACGGTACTA CATGGGGTGC CTATGGTTCT AATTACTCTG GAGACAAATG TTCAAGCCCT 
AATGGCCAAG CTAATGGTGA CACTGTGAAT GCAACTCTAA TGCAGCCTGG CGTAAACGGG 
CCTATGGGCA CTAACTTTCA AGTTAACACA AACAAAGGAG GCGGCGTGTG GGAGTCTGGA 
GCAGTGAATT CCCAGAGTGC ATCCTGGGGA AATGGTGCAA ATTCTGGAGG AAGTCGAAGA 
GGGTGGGGAA CTCCTGCACA AAACACTGGC ACTAATACAC CCGGCGTGGA GTGGAACAAA 
CTGCCTGGCA ACCAGCATTC CAGTGACAGT GCAAATGGCA ACGGTAAGAA GTTTACAAAC 
GGATGGAAGT CTACTGAGGA AGAGGATCAG GGTTCTGCCG CATCTCAGAC AAATGAGCAA 
AGCAGTGTGT GGGCCAAAAC AGGAGGTACA GTGGAGAGTG AAGGTAGTAC AGAAAGCACT 
GGACGCCTCG AGGAGAAAGC GACTGGGGAA AATCAGAGTA GGGACAGAAG GAAGATGGAT 
CAGCACACGT TACTCCAAAG CATTGTAAAC AGAACTGACT TAGATCCACG TGTCCTGTCC 
AACTCTGGCT GGGGACAGAC TCCTATTAAG CAGAATACTG CCTGGGATAC AGAAACATCA 
CCAAGAGGGG AGAGAAAGAC TGACAATGGG ACAGAGGCCT GGGGAAGCTC TGCAACACAG 
ACTTTTAACT CAGGGGCGTG TGTAGATAAG ACTAGCCCGA GTAGTAATGA TACCTCATCT 
GTATCGGGGT GGGGAGATCC CAAACCTGCT CTGCGGTGGG GAGATTCCAA AGGCTCAAGC 
TGCCAGGGGG GATGGGAAGA TGACTCTGCT GCTACAGGAA TGGTCAAGAG CAATCAGTGG 
GGGAACTGCA AAGAAGAGAA GTCCGCATGG AATGATTCGC AAAAGAACAA ACAGGGATGG 
GGTGACGGAC AAAAGTCAAA CCAAGGGTGG TCCGTCTCTG CCGGTGACAA CTGGGGAGAA 
AGGAGTAACC ATTGGGGAGA GGCTAACAAG AAGTCTAGCT CAGGAGGTAG TGACAGTGAT 
AGGTCTGTTT CTGGTTGGAA TGAACTCGGT AAAACCAGTT CTTTTACATG GGGAAATAAC 
ATTAATCCAA ATAATTCATC AGGCTGGGAC GAATCTTCTA AACCGAATCC TTCCCAGGGA 
TGGGGAGACC CTCCAAAGTC TAATCAGTCT CTAGGTTGGG GAGATTCATC AAAGCCGGTC 
AGTTCTCCAG ACTGGAACAA GCAACAAGAC ATTGTCGGAT CTTGGGGCAT CCCGCCAGCG 
ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG GGAGGACCTA TGCCAGCCCC AGCGAAAGAG 
GAAGAACCCA CGGGCTGGGA GGAGCCATCC CCAGAATCCA TACGTCGCAA AATGGAGATT 
GACGATGGGA CTTCAGCTTG GGGAGATCCC AGCAAATACA ATTACAAAAA TGTGAACATG 
TGGAACAAAA ACGTCCCAAA TGGCAGCAGT CGCTCAGACC AGCAAGCACA GGTACACCAG 
TTGCTACCGT CTGCAGGTAC CATCTCAAAC AAAGAGGCGA GCAGTGGCTC CGGCTGGGGT 
GAGCCCTGGG GGGAGACTTC AACTCCAGCC ACAACTGTGG ATAATGGTAC TTCAGCCTGG 
GGTAAACCCG TAGACAGTGG GCCTAGCTGG GGCGAACCCA TTGCTGCGGC ATCCAGCACA 
TCCACGTGGG GCTCCAGCTC TGTTGGTCCA CAAACATTAA ACAAATCTGG GCCAAAATCT 
ATGCAAGACG GCTGGTGTGG TGATGATATG CCCTTGCCTG GAAATCGCCC CACTGGCTGG 
GAAGAGGAAG AAGATGTAGA GATTGGAATG TGGAATAGTA ATTCATCTCA AGAGCTTAAT 
TCATCTTTAA ATTGGCCACC ATATACAAAG AAAATGTCTT CGAAGGGTCT GAGTGGCAAA 
AAAAGGAGAA GGGAAAGGGG AATGATGAAA GGTGGAAACA AACAAGAAGA AGCATGGATA 
AATCCATTTG TTAAACAGTT TTCAAATATC AGTTTTTCGA GAGACTCGCC AGAAGAAAAT 
GTACAGAGCA ATAAGATGGA CCTTTCTGGA GGAATGTTAC AAGACAAACG AATGGAGATA 
GATAAACATA GCCTAAATAT TGGTGATTAC AATCGAACGG TCGGGAAAGG CCCTGGTTCT 
CGGCCTCAGA TTTCCAAAGA GTCTTCCATG GACCGCAGTC CTTATTTTGA TAAGGATGGC 
ATTGTAGCAG ATGAATCCCA AAACATGCAG TTTATGTCCA GTCAAAGCAT GAAGCTTCCC 
CCTTCAAATA GTGCACTACC TAACCAGGCC CTGGGCTCCA TAGCAGGGCT GGGTATGCAA 
AACTTGAATT CTGTTAGACA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_010819373.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817675.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OBa54984
Clone ID Related Accession (Same CDS sequence)	XM_010819373.2 , XM_015469344.1
Accession Version	XM_010819373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGGA TGGCATTGTA 
GCAGATGAAT CCCAAAACAT GCAGTTTATG TCCAGTCAAA GCATGAAGCT TCCCCCTTCA 
AATAGTGCAC TACCTAACCA GGCCCTGGGC TCCATAGCAG GGCTGGGTAT GCAAAACTTG 
AATTCTGTTA GACAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817675.1
CDS	428..6247
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_010819373.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819373.2

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGGA TGGCATTGTA 
GCAGATGAAT CCCAAAACAT GCAGTTTATG TCCAGTCAAA GCATGAAGCT TCCCCCTTCA 
AATAGTGCAC TACCTAACCA GGCCCTGGGC TCCATAGCAG GGCTGGGTAT GCAAAACTTG 
AATTCTGTTA GACAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469344.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324830.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OBa54984
Clone ID Related Accession (Same CDS sequence)	XM_010819373.2 , XM_015469344.1
Accession Version	XM_015469344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGGA TGGCATTGTA 
GCAGATGAAT CCCAAAACAT GCAGTTTATG TCCAGTCAAA GCATGAAGCT TCCCCCTTCA 
AATAGTGCAC TACCTAACCA GGCCCTGGGC TCCATAGCAG GGCTGGGTAT GCAAAACTTG 
AATTCTGTTA GACAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324830.1
CDS	428..6247
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469344.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469344.1

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGAATGAT GAAAGGTGGA AACAAACAAG AAGAAGCATG GATAAATCCA 
TTTGTTAAAC AGTTTTCAAA TATCAGTTTT TCGAGAGACT CGCCAGAAGA AAATGTACAG 
AGCAATAAGA TGGACCTTTC TGGAGGAATG TTACAAGACA AACGAATGGA GATAGATAAA 
CATAGCCTAA ATATTGGTGA TTACAATCGA ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT 
CAGATTTCCA AAGAGTCTTC CATGGACCGC AGTCCTTATT TTGATAAGGA TGGCATTGTA 
GCAGATGAAT CCCAAAACAT GCAGTTTATG TCCAGTCAAA GCATGAAGCT TCCCCCTTCA 
AATAGTGCAC TACCTAACCA GGCCCTGGGC TCCATAGCAG GGCTGGGTAT GCAAAACTTG 
AATTCTGTTA GACAGAATGG CAATCCCAGT ATGTTTGGTG TTGGAAACAC AGCAGCACAA 
CCCCGGGGCA TGCAGCAGCC TCCAGCACAA CCTCTCAGTT CATCTCAGCC TAGTCTCCGT 
GCTCAAGTGC CTCCTCCATT ACTCTCCCCT CAGGTTCCAG TTTCATTGCT GAAGTATGCA 
CCAAACAACG GTGGCCTGAA TCCGCTCTTT GGCCCTCAAC AGGTAGCCAT GCTGAACCAG 
CTATCCCAAC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCAGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGCGTGCA GCAGCAGATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAACAAAC TCCGCCATCT CAGCAACAGT CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGAAAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACTAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGC CCTGGTGAAC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGCT 
CCCCTCAGCA GCACAGCACA AAGCACTGCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCCCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTCTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTTCGT GGGATAATTC TCCCCTTCGA GTAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGGTATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATTGAT GGCTCAACTC TGCGTACTCT GTGCATGCAG 
CATGGCCCAC TGATAACATT CCACCTGAAC CTCCCACATG GAAACGCTTT GGTCCGTTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGT 
CAGTCTCTGA CCCCTTCTCC CAGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CATGGCACTT CCCTCTGGGG GACCCCACAT 
TATTCCACAA GCCTGTGGGG TCCTCCCAGC AGCAGCGACC CCCGGGGAAT TAGCAGCCCA 
TCCCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469345.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324831.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OBa54985
Clone ID Related Accession (Same CDS sequence)	XM_015469345.1 , XM_010819375.2
Accession Version	XM_015469345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324831.1
CDS	427..6132
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469345.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469345.1

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_010819375.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817677.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OBa54985
Clone ID Related Accession (Same CDS sequence)	XM_015469345.1 , XM_010819375.2
Accession Version	XM_010819375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817677.1
CDS	427..6132
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_010819375.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819375.2

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GAATGGCAAT CCCAGTATGT TTGGTGTTGG AAACACAGCA 
GCACAACCCC GGGGCATGCA GCAGCCTCCA GCACAACCTC TCAGTTCATC TCAGCCTAGT 
CTCCGTGCTC AAGTGCCTCC TCCATTACTC TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG 
TATGCACCAA ACAACGGTGG CCTGAATCCG CTCTTTGGCC CTCAACAGGT AGCCATGCTG 
AACCAGCTAT CCCAACTAAA CCAGCTTTCT CAGATCTCCC AGTTACAGCG ATTGTTAGCG 
CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC GTGCCTTCTG GGAACCGGCA GCAGCAAGAC 
CAGCAGGGTC GACCTCTTAG CGTGCAGCAG CAGATGATGC AACAATCTCG TCAACTTGAT 
CCAAACCTGT TGGTGAAGCA ACAAACTCCG CCATCTCAGC AACAGTCACT CCATCAGCCA 
GCCATGAAGT CTTTCCTTGA AAATGTCATG CCCCACACTA CACCTGAGCT GCAAAAAGGG 
CCATCACCAA TAAATGCTTT CAGCAACTTC CCTATAGGCT TGAACTCAAA CTTGAATGTA 
AATATGGATA TGAACAGTAT TAAAGAGCCA CAGTCAAGAC TAAGGAAGTG GACTACAGTG 
GACAGCATTT CTGTGAACAC ATCTTTGGAT CAAAACTCCA GCAAACATGG TGCTATTTCA 
AGTGGTTTCA GGCTGGAAGA GTCTCCATTT GTTCCCTATG ACTTTATGAA CAGCAGTACT 
TCACCAGCCA GTCCTCCAGG TTCAATAGGA GATGGCTGGC CACGTGCCAA ATCGCCTAAC 
GGCTCTAGCA GTGTTAATTG GCCACCAGAA TTTCGCCCTG GTGAACCATG GAAAGGTTAT 
CCAAACATTG ACCCTGAAAC TGACCCTTAC GTCACTCCTG GCAGTGTCAT AAACAATCTT 
TCAATTAATA CTGTGCGGGA AGTTGACCAC CTCAGGGACA GGAACAGTGG GTCATCCTCA 
TCCTTGAACA CCACGCTGCC TTCAACTAGT GCCTGGTCAT CCATTCGTGC CTCCAACTAC 
AACGCTCCCC TCAGCAGCAC AGCACAAAGC ACTGCAGCCA GAAATAGTGA TTCCAAATTG 
ACATGGTCCC CTGGTTCAGT TACAAACACC TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT 
CTGCCACCTA AAAACATCAC TGCTCCGTCC CGCCCACCTC CGGGACTGAC TGGTCAGAAG 
CCACCCTTGT CTTCGTGGGA TAATTCTCCC CTTCGAGTAG GTGGAGGATG GGGAAATTCT 
GACGCCAGGT ATACCCCAGG TTCCAGCTGG GGTGAGAGCA GCTCAGGGAG AATAACAAAT 
TGGCTTGTTC TAAAAAACCT TACACCTCAG ATTGATGGCT CAACTCTGCG TACTCTGTGC 
ATGCAGCATG GCCCACTGAT AACATTCCAC CTGAACCTCC CACATGGAAA CGCTTTGGTC 
CGTTACAGTT CAAAAGAAGA GGTAGTGAAG GCACAAAAGT CTCTGCACAT GTGTGTACTG 
GGGAACACTA CTATTCTTGC TGAGTTTGCC AGTGAAGAGG AGATCAGTCG TTTCTTTGCA 
CAAAGTCAGT CTCTGACCCC TTCTCCCAGC TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG 
CTGGGCTCCC TCGACTGTTC CCACTCATTC TCCAGCCGGA CCGATCTCAA TCACTGGAAT 
GGTGCTGGGC TGTCGGGAAC TAACTGTGGA GACCTTCATG GCACTTCCCT CTGGGGGACC 
CCACATTATT CCACAAGCCT GTGGGGTCCT CCCAGCAGCA GCGACCCCCG GGGAATTAGC 
AGCCCATCCC CCATTAACGC TTTTCTTTCT GTTGACCACC TGGGTGGGGG TGGAGAGTCC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469346.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324832.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa54986
Clone ID Related Accession (Same CDS sequence)	XM_015469346.1 , XM_010819377.2
Accession Version	XM_015469346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG GCCAAAATCT ATGCAAGACG GCTGGTGTGG TGATGATATG 
CCCTTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AAGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAT TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCTT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTTG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCGCC AGAAGAAAAT GTACAGAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GACCGCAGTC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTGGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TCAGTTCATC TCAGCCTAGT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA ACAAACTCCG 
CCATCTCAGC AACAGTCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA AAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGCTCCCC TCAGCAGCAC AGCACAAAGC 
ACTGCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCCC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT CTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTTCGTGGGA TAATTCTCCC 
CTTCGAGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATTGATGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA CGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCAGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCATG GCACTTCCCT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324832.1
CDS	428..6103
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469346.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469346.1

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG GCCAAAATCT ATGCAAGACG GCTGGTGTGG TGATGATATG 
CCCTTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AAGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAT TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCTT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTTG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCGCC AGAAGAAAAT GTACAGAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GACCGCAGTC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTGGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TCAGTTCATC TCAGCCTAGT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA ACAAACTCCG 
CCATCTCAGC AACAGTCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA AAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGCTCCCC TCAGCAGCAC AGCACAAAGC 
ACTGCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCCC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT CTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTTCGTGGGA TAATTCTCCC 
CTTCGAGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATTGATGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA CGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCAGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCATG GCACTTCCCT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_010819377.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817679.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa54986
Clone ID Related Accession (Same CDS sequence)	XM_015469346.1 , XM_010819377.2
Accession Version	XM_010819377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG GCCAAAATCT ATGCAAGACG GCTGGTGTGG TGATGATATG 
CCCTTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AAGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAT TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCTT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTTG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCGCC AGAAGAAAAT GTACAGAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GACCGCAGTC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTGGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TCAGTTCATC TCAGCCTAGT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA ACAAACTCCG 
CCATCTCAGC AACAGTCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA AAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGCTCCCC TCAGCAGCAC AGCACAAAGC 
ACTGCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCCC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT CTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTTCGTGGGA TAATTCTCCC 
CTTCGAGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATTGATGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA CGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCAGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCATG GCACTTCCCT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817679.1
CDS	428..6103
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_010819377.2
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819377.2

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG GCCAAAATCT ATGCAAGACG GCTGGTGTGG TGATGATATG 
CCCTTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AAGATGTAGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAT TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCTT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCATGGATA AATCCATTTG TTAAACAGTT TTCAAATATC 
AGTTTTTCGA GAGACTCGCC AGAAGAAAAT GTACAGAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GACCGCAGTC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTGGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TCAGTTCATC TCAGCCTAGT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCG 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAACTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCA GCAGCAAGAC CAGCAGGGTC GACCTCTTAG CGTGCAGCAG 
CAGATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA ACAAACTCCG 
CCATCTCAGC AACAGTCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA AAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACTACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGCCCTG GTGAACCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGCTCCCC TCAGCAGCAC AGCACAAAGC 
ACTGCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCCC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT CTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTTCGTGGGA TAATTCTCCC 
CTTCGAGTAG GTGGAGGATG GGGAAATTCT GACGCCAGGT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATTGATGGCT CAACTCTGCG TACTCTGTGC ATGCAGCATG GCCCACTGAT AACATTCCAC 
CTGAACCTCC CACATGGAAA CGCTTTGGTC CGTTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGTCAGT CTCTGACCCC TTCTCCCAGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCATG GCACTTCCCT CTGGGGGACC CCACATTATT CCACAAGCCT GTGGGGTCCT 
CCCAGCAGCA GCGACCCCCG GGGAATTAGC AGCCCATCCC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469342.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324828.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

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CloneID	OBa88502
Clone ID Related Accession (Same CDS sequence)	XM_015469342.1 , XM_015460350.1
Accession Version	XM_015469342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTG 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324828.1
CDS	428..6280
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469342.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469342.1

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTG 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015460350.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315836.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

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CloneID	OBa88502
Clone ID Related Accession (Same CDS sequence)	XM_015469342.1 , XM_015460350.1
Accession Version	XM_015460350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTG 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315836.1
CDS	428..6280
Translation	MQHYCYSFILGKLIATVWFRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015460350.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460350.1

ORF Insert Sequence:

ATGCAACATT ACTGTTATTC ATTCATTTTG GGAAAGTTAA TTGCAACTGT GTGGTTCAGG 
GATTTAGTGC AAGAAGAAGA ACAGTTGATG GAAGAAAAGA AAAAGAAAAA AGACGACAAG 
AAAAAGAAGG AAGCTGCTCA AAAGAAGGCC ACTGAACAAA AAATCAAAGT GCCAGAACAG 
ATAAAGCCCA GTGTAAGCCA GCCTCAGCCT GCCAACTCTA ATAACGGCAC TTCCACAGCA 
ACCAGCACTA ATAATAATGC CAAGCGAGCC ACAGCCAACA ATCAGCAGCA GCAGCAGCAA 
CAGCCGCAAC CGCAGCAGCA GCAACCGCAG CAGCCGCAGC AGCCGCAGCC GCCACCACAG 
GCCTTGCCTC GGTATCCCCG GGAAGTACCA CCTCGATTTC GCCACCAGGA ACATAAGCAG 
CTTCTAAAGA GGGGTCAGCA TTTTCCCGTC ATAGCAGCAA ACCTGGGATC TGCTGTTAAA 
GTGTTAAGCA GCCAGTCAGA AAGCAGTGCT TTAACAAATC AACAGCCACA AAATAACGGA 
GAGGTGCAGA TCAGCAAAAA CCAGTCAGAT ACAAACCACA ATACTTCAGG ATCCCATTAT 
GAAAATTCAG AGCGGGGACC TGTGTCGTCT ACAAGTGACT CTCGTACAAA CTGTAAGAAT 
GCTGTTGTGA ACGACTCCCC TGAAAAAGAA GCATGGCCCT CAGCCCCTGG CAGTGATCCG 
GAGTTGGTTT CAGAATGTAT GGATGCTGAT TCTGCCTCCA GTTCTGAATC AGAGCGACCC 
ATCACTATCA TGGCTTCAGG GAGCACAGGT GGTGAAAATG ATGGCCTTCG GAATAGCACT 
GGACTTGGAT CCCAAAACAA GTTTGTGGTT GGTAGCAGCA GCAATAATGT GGGCCACGGA 
AGTAGCACTG GGCCGTGGGG TTTTTCCCAT GGAGCCATAA TAAGCACATG TCAGGCCTCT 
GTGGATGCTC CTGAAAGCAA ATCAGAAAGT AGCAACAATA GAATGAATGC TTGGGGCACT 
GTAAGTTCTT CATCAAATGG AGGGTTAAAT CCAAGCACTT TGAATTCAGC TAGCAACCAT 
GGTGCCTGGC CAGTATTAGA GAACAACGGA CTTGCCCTAA AAGGGCCTGT AGGGAGTGGT 
AGTTCTGGCA TCAATATTCA GTGCAGTCCC TTAGGCCAGA TGCCTAACAA TCAGAGTATT 
AACTCTAAAG TGGGTGGTGC TTCCACCCAT GGTACCTGGG GAAGCCTTCA GGAAACTTGT 
GAATCTGAAG TAAGTGGTAC ACAGAAGGTT TCATTCAGTG GTCAACCTCA AAATATTACC 
ACTGAAATGA CTGGACCAAA TAACACTACT AACTTTATGA CCTCTAGTTT ACCAAACTCC 
GGTTCAGTAC AGAATAATGA ACTGCCTAGT AATACAGGGG CCTGGCGTGT GAGCACAATG 
AATCATCCTC AGATACAGGC TCCCTCGGTT GTAAATGGCA CTTCCCTTTC TCACCTTAGC 
AATGGAGAGT CAAAAAGTGG AGGCTCTTAC GGTACTACAT GGGGTGCCTA TGGTTCTAAT 
TACTCTGGAG ACAAATGTTC AAGCCCTAAT GGCCAAGCTA ATGGTGACAC TGTGAATGCA 
ACTCTAATGC AGCCTGGCGT AAACGGGCCT ATGGGCACTA ACTTTCAAGT TAACACAAAC 
AAAGGAGGCG GCGTGTGGGA GTCTGGAGCA GTGAATTCCC AGAGTGCATC CTGGGGAAAT 
GGTGCAAATT CTGGAGGAAG TCGAAGAGGG TGGGGAACTC CTGCACAAAA CACTGGCACT 
AATACACCCG GCGTGGAGTG GAACAAACTG CCTGGCAACC AGCATTCCAG TGACAGTGCA 
AATGGCAACG GTAAGAAGTT TACAAACGGA TGGAAGTCTA CTGAGGAAGA GGATCAGGGT 
TCTGCCGCAT CTCAGACAAA TGAGCAAAGC AGTGTGTGGG CCAAAACAGG AGGTACAGTG 
GAGAGTGAAG GTAGTACAGA AAGCACTGGA CGCCTCGAGG AGAAAGCGAC TGGGGAAAAT 
CAGAGTAGGG ACAGAAGGAA GATGGATCAG CACACGTTAC TCCAAAGCAT TGTAAACAGA 
ACTGACTTAG ATCCACGTGT CCTGTCCAAC TCTGGCTGGG GACAGACTCC TATTAAGCAG 
AATACTGCCT GGGATACAGA AACATCACCA AGAGGGGAGA GAAAGACTGA CAATGGGACA 
GAGGCCTGGG GAAGCTCTGC AACACAGACT TTTAACTCAG GGGCGTGTGT AGATAAGACT 
AGCCCGAGTA GTAATGATAC CTCATCTGTA TCGGGGTGGG GAGATCCCAA ACCTGCTCTG 
CGGTGGGGAG ATTCCAAAGG CTCAAGCTGC CAGGGGGGAT GGGAAGATGA CTCTGCTGCT 
ACAGGAATGG TCAAGAGCAA TCAGTGGGGG AACTGCAAAG AAGAGAAGTC CGCATGGAAT 
GATTCGCAAA AGAACAAACA GGGATGGGGT GACGGACAAA AGTCAAACCA AGGGTGGTCC 
GTCTCTGCCG GTGACAACTG GGGAGAAAGG AGTAACCATT GGGGAGAGGC TAACAAGAAG 
TCTAGCTCAG GAGGTAGTGA CAGTGATAGG TCTGTTTCTG GTTGGAATGA ACTCGGTAAA 
ACCAGTTCTT TTACATGGGG AAATAACATT AATCCAAATA ATTCATCAGG CTGGGACGAA 
TCTTCTAAAC CGAATCCTTC CCAGGGATGG GGAGACCCTC CAAAGTCTAA TCAGTCTCTA 
GGTTGGGGAG ATTCATCAAA GCCGGTCAGT TCTCCAGACT GGAACAAGCA ACAAGACATT 
GTCGGATCTT GGGGCATCCC GCCAGCGACA GGCAAACCTC CTGGTACAGG CTGGCTGGGA 
GGACCTATGC CAGCCCCAGC GAAAGAGGAA GAACCCACGG GCTGGGAGGA GCCATCCCCA 
GAATCCATAC GTCGCAAAAT GGAGATTGAC GATGGGACTT CAGCTTGGGG AGATCCCAGC 
AAATACAATT ACAAAAATGT GAACATGTGG AACAAAAACG TCCCAAATGG CAGCAGTCGC 
TCAGACCAGC AAGCACAGGT ACACCAGTTG CTACCGTCTG CAGGTACCAT CTCAAACAAA 
GAGGCGAGCA GTGGCTCCGG CTGGGGTGAG CCCTGGGGGG AGACTTCAAC TCCAGCCACA 
ACTGTGGATA ATGGTACTTC AGCCTGGGGT AAACCCGTAG ACAGTGGGCC TAGCTGGGGC 
GAACCCATTG CTGCGGCATC CAGCACATCC ACGTGGGGCT CCAGCTCTGT TGGTCCACAA 
ACATTAAACA AATCTGGGCC AAAATCTATG CAAGACGGCT GGTGTGGTGA TGATATGCCC 
TTGCCTGGAA ATCGCCCCAC TGGCTGGGAA GAGGAAGAAG ATGTAGAGAT TGGAATGTGG 
AATAGTAATT CATCTCAAGA GCTTAATTCA TCTTTAAATT GGCCACCATA TACAAAGAAA 
ATGTCTTCGA AGGGTCTGAG TGGCAAAAAA AGGAGAAGGG AAAGGGGAAT GATGAAAGGT 
GGAAACAAAC AAGAAGAAGC ATGGATAAAT CCATTTGTTA AACAGTTTTC AAATATCAGT 
TTTTCGAGAG ACTCGCCAGA AGAAAATGTA CAGAGCAATA AGATGGACCT TTCTGGAGGA 
ATGTTACAAG ACAAACGAAT GGAGATAGAT AAACATAGCC TAAATATTGG TGATTACAAT 
CGAACGGTCG GGAAAGGCCC TGGTTCTCGG CCTCAGATTT CCAAAGAGTC TTCCATGGAC 
CGCAGTCCTT ATTTTGATAA GGATGGCATT GTAGCAGATG AATCCCAAAA CATGCAGTTT 
ATGTCCAGTC AAAGCATGAA GCTTCCCCCT TCAAATAGTG CACTACCTAA CCAGGCCCTG 
GGCTCCATAG CAGGGCTGGG TATGCAAAAC TTGAATTCTG TTAGACAGAA TGGCAATCCC 
AGTATGTTTG GTGTTGGAAA CACAGCAGCA CAACCCCGGG GCATGCAGCA GCCTCCAGCA 
CAACCTCTCA GTTCATCTCA GCCTAGTCTC CGTGCTCAAG TGCCTCCTCC ATTACTCTCC 
CCTCAGGTTC CAGTTTCATT GCTGAAGTAT GCACCAAACA ACGGTGGCCT GAATCCGCTC 
TTTGGCCCTC AACAGGTAGC CATGCTGAAC CAGCTATCCC AACTAAACCA GCTTTCTCAG 
ATCTCCCAGT TACAGCGATT GTTAGCGCAG CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG 
CCTTCTGGGA ACCGGCAGCA GCAAGACCAG CAGGGTCGAC CTCTTAGCGT GCAGCAGCAG 
ATGATGCAAC AATCTCGTCA ACTTGATCCA AACCTGTTGG TGAAGCAACA AACTCCGCCA 
TCTCAGCAAC AGTCACTCCA TCAGCCAGCC ATGAAGTCTT TCCTTGAAAA TGTCATGCCC 
CACACTACAC CTGAGCTGCA AAAAGGGCCA TCACCAATAA ATGCTTTCAG CAACTTCCCT 
ATAGGCTTGA ACTCAAACTT GAATGTAAAT ATGGATATGA ACAGTATTAA AGAGCCACAG 
TCAAGACTAA GGAAGTGGAC TACAGTGGAC AGCATTTCTG TGAACACATC TTTGGATCAA 
AACTCCAGCA AACATGGTGC TATTTCAAGT GGTTTCAGGC TGGAAGAGTC TCCATTTGTT 
CCCTATGACT TTATGAACAG CAGTACTTCA CCAGCCAGTC CTCCAGGTTC AATAGGAGAT 
GGCTGGCCAC GTGCCAAATC GCCTAACGGC TCTAGCAGTG TTAATTGGCC ACCAGAATTT 
CGCCCTGGTG AACCATGGAA AGGTTATCCA AACATTGACC CTGAAACTGA CCCTTACGTC 
ACTCCTGGCA GTGTCATAAA CAATCTTTCA ATTAATACTG TGCGGGAAGT TGACCACCTC 
AGGGACAGGA ACAGTGGGTC ATCCTCATCC TTGAACACCA CGCTGCCTTC AACTAGTGCC 
TGGTCATCCA TTCGTGCCTC CAACTACAAC GCTCCCCTCA GCAGCACAGC ACAAAGCACT 
GCAGCCAGAA ATAGTGATTC CAAATTGACA TGGTCCCCTG GTTCAGTTAC AAACACCTCT 
CTGGCTCATG AGCTGTGGAA GGTCCCTCTG CCACCTAAAA ACATCACTGC TCCGTCCCGC 
CCACCTCCGG GACTGACTGG TCAGAAGCCA CCCTTGTCTT CGTGGGATAA TTCTCCCCTT 
CGAGTAGGTG GAGGATGGGG AAATTCTGAC GCCAGGTATA CCCCAGGTTC CAGCTGGGGT 
GAGAGCAGCT CAGGGAGAAT AACAAATTGG CTTGTTCTAA AAAACCTTAC ACCTCAGATT 
GATGGCTCAA CTCTGCGTAC TCTGTGCATG CAGCATGGCC CACTGATAAC ATTCCACCTG 
AACCTCCCAC ATGGAAACGC TTTGGTCCGT TACAGTTCAA AAGAAGAGGT AGTGAAGGCA 
CAAAAGTCTC TGCACATGTG TGTACTGGGG AACACTACTA TTCTTGCTGA GTTTGCCAGT 
GAAGAGGAGA TCAGTCGTTT CTTTGCACAA AGTCAGTCTC TGACCCCTTC TCCCAGCTGG 
CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG GGCTCCCTCG ACTGTTCCCA CTCATTCTCC 
AGCCGGACCG ATCTCAATCA CTGGAATGGT GCTGGGCTGT CGGGAACTAA CTGTGGAGAC 
CTTCATGGCA CTTCCCTCTG GGGGACCCCA CATTATTCCA CAAGCCTGTG GGGTCCTCCC 
AGCAGCAGCG ACCCCCGGGG AATTAGCAGC CCATCCCCCA TTAACGCTTT TCTTTCTGTT 
GACCACCTGG GTGGGGGTGG AGAGTCCATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015460351.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315837.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OBa88503
Clone ID Related Accession (Same CDS sequence)	XM_015460351.1 , XM_015469347.1
Accession Version	XM_015460351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TGGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT CAGTTCATCT 
CAGCCTAGTC TCCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGCGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA ACAATCTCGT 
CAACTTGATC CAAACCTGTT GGTGAAGCAA CAAACTCCGC CATCTCAGCA ACAGTCACTC 
CATCAGCCAG CCATGAAGTC TTTCCTTGAA AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAAAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAATTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGCTCCCCT CAGCAGCACA GCACAAAGCA CTGCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCCCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTC TGCCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTGACT 
GGTCAGAAGC CACCCTTGTC TTCGTGGGAT AATTCTCCCC TTCGAGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA TACCCCAGGT TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAAAACCTT ACACCTCAGA TTGATGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAC 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTACTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCAGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCATGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAACGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315837.1
CDS	202..5316
Translation	MDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015460351.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460351.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TGGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT CAGTTCATCT 
CAGCCTAGTC TCCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGCGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA ACAATCTCGT 
CAACTTGATC CAAACCTGTT GGTGAAGCAA CAAACTCCGC CATCTCAGCA ACAGTCACTC 
CATCAGCCAG CCATGAAGTC TTTCCTTGAA AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAAAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAATTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGCTCCCCT CAGCAGCACA GCACAAAGCA CTGCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCCCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTC TGCCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTGACT 
GGTCAGAAGC CACCCTTGTC TTCGTGGGAT AATTCTCCCC TTCGAGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA TACCCCAGGT TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAAAACCTT ACACCTCAGA TTGATGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAC 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTACTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCAGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCATGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAACGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469347.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324833.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OBa88503
Clone ID Related Accession (Same CDS sequence)	XM_015460351.1 , XM_015469347.1
Accession Version	XM_015469347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TGGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT CAGTTCATCT 
CAGCCTAGTC TCCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGCGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA ACAATCTCGT 
CAACTTGATC CAAACCTGTT GGTGAAGCAA CAAACTCCGC CATCTCAGCA ACAGTCACTC 
CATCAGCCAG CCATGAAGTC TTTCCTTGAA AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAAAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAATTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGCTCCCCT CAGCAGCACA GCACAAAGCA CTGCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCCCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTC TGCCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTGACT 
GGTCAGAAGC CACCCTTGTC TTCGTGGGAT AATTCTCCCC TTCGAGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA TACCCCAGGT TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAAAACCTT ACACCTCAGA TTGATGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAC 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTACTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCAGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCATGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAACGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324833.1
CDS	219..5333
Translation	MDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469347.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469347.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGCGAC CCATCACTAT CATGGCTTCA 
GGGAGCACAG GTGGTGAAAA TGATGGCCTT CGGAATAGCA CTGGACTTGG ATCCCAAAAC 
AAGTTTGTGG TTGGTAGCAG CAGCAATAAT GTGGGCCACG GAAGTAGCAC TGGGCCGTGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGCCT CTGTGGATGC TCCTGAAAGC 
AAATCAGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAACG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATCAATATT 
CAGTGCAGTC CCTTAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGGGTGGT 
GCTTCCACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT ACAGAATAAT 
GAACTGCCTA GTAATACAGG GGCCTGGCGT GTGAGCACAA TGAATCATCC TCAGATACAG 
GCTCCCTCGG TTGTAAATGG CACTTCCCTT TCTCACCTTA GCAATGGAGA GTCAAAAAGT 
GGAGGCTCTT ACGGTACTAC ATGGGGTGCC TATGGTTCTA ATTACTCTGG AGACAAATGT 
TCAAGCCCTA ATGGCCAAGC TAATGGTGAC ACTGTGAATG CAACTCTAAT GCAGCCTGGC 
GTAAACGGGC CTATGGGCAC TAACTTTCAA GTTAACACAA ACAAAGGAGG CGGCGTGTGG 
GAGTCTGGAG CAGTGAATTC CCAGAGTGCA TCCTGGGGAA ATGGTGCAAA TTCTGGAGGA 
AGTCGAAGAG GGTGGGGAAC TCCTGCACAA AACACTGGCA CTAATACACC CGGCGTGGAG 
TGGAACAAAC TGCCTGGCAA CCAGCATTCC AGTGACAGTG CAAATGGCAA CGGTAAGAAG 
TTTACAAACG GATGGAAGTC TACTGAGGAA GAGGATCAGG GTTCTGCCGC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGTGA AGGTAGTACA 
GAAAGCACTG GACGCCTCGA GGAGAAAGCG ACTGGGGAAA ATCAGAGTAG GGACAGAAGG 
AAGATGGATC AGCACACGTT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGCTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CAAGAGGGGA GAGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCGTGT GTAGATAAGA CTAGCCCGAG TAGTAATGAT 
ACCTCATCTG TATCGGGGTG GGGAGATCCC AAACCTGCTC TGCGGTGGGG AGATTCCAAA 
GGCTCAAGCT GCCAGGGGGG ATGGGAAGAT GACTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAACTGCAA AGAAGAGAAG TCCGCATGGA ATGATTCGCA AAAGAACAAA 
CAGGGATGGG GTGACGGACA AAAGTCAAAC CAAGGGTGGT CCGTCTCTGC CGGTGACAAC 
TGGGGAGAAA GGAGTAACCA TTGGGGAGAG GCTAACAAGA AGTCTAGCTC AGGAGGTAGT 
GACAGTGATA GGTCTGTTTC TGGTTGGAAT GAACTCGGTA AAACCAGTTC TTTTACATGG 
GGAAATAACA TTAATCCAAA TAATTCATCA GGCTGGGACG AATCTTCTAA ACCGAATCCT 
TCCCAGGGAT GGGGAGACCC TCCAAAGTCT AATCAGTCTC TAGGTTGGGG AGATTCATCA 
AAGCCGGTCA GTTCTCCAGA CTGGAACAAG CAACAAGACA TTGTCGGATC TTGGGGCATC 
CCGCCAGCGA CAGGCAAACC TCCTGGTACA GGCTGGCTGG GAGGACCTAT GCCAGCCCCA 
GCGAAAGAGG AAGAACCCAC GGGCTGGGAG GAGCCATCCC CAGAATCCAT ACGTCGCAAA 
ATGGAGATTG ACGATGGGAC TTCAGCTTGG GGAGATCCCA GCAAATACAA TTACAAAAAT 
GTGAACATGT GGAACAAAAA CGTCCCAAAT GGCAGCAGTC GCTCAGACCA GCAAGCACAG 
GTACACCAGT TGCTACCGTC TGCAGGTACC ATCTCAAACA AAGAGGCGAG CAGTGGCTCC 
GGCTGGGGTG AGCCCTGGGG GGAGACTTCA ACTCCAGCCA CAACTGTGGA TAATGGTACT 
TCAGCCTGGG GTAAACCCGT AGACAGTGGG CCTAGCTGGG GCGAACCCAT TGCTGCGGCA 
TCCAGCACAT CCACGTGGGG CTCCAGCTCT GTTGGTCCAC AAACATTAAA CAAATCTGGG 
CCAAAATCTA TGCAAGACGG CTGGTGTGGT GATGATATGC CCTTGCCTGG AAATCGCCCC 
ACTGGCTGGG AAGAGGAAGA AGATGTAGAG ATTGGAATGT GGAATAGTAA TTCATCTCAA 
GAGCTTAATT CATCTTTAAA TTGGCCACCA TATACAAAGA AAATGTCTTC GAAGGGTCTG 
AGTGGCAAAA AAAGGAGAAG GGAAAGGGGA ATGATGAAAG GTGGAAACAA ACAAGAAGAA 
GCATGGATAA ATCCATTTGT TAAACAGTTT TCAAATATCA GTTTTTCGAG AGACTCGCCA 
GAAGAAAATG TACAGAGCAA TAAGATGGAC CTTTCTGGAG GAATGTTACA AGACAAACGA 
ATGGAGATAG ATAAACATAG CCTAAATATT GGTGATTACA ATCGAACGGT CGGGAAAGGC 
CCTGGTTCTC GGCCTCAGAT TTCCAAAGAG TCTTCCATGG ACCGCAGTCC TTATTTTGAT 
AAGGATGGCA TTGTAGCAGA TGAATCCCAA AACATGCAGT TTATGTCCAG TCAAAGCATG 
AAGCTTCCCC CTTCAAATAG TGCACTACCT AACCAGGCCC TGGGCTCCAT AGCAGGGCTG 
GGTATGCAAA ACTTGAATTC TGTTAGACAG AATGGCAATC CCAGTATGTT TGGTGTTGGA 
AACACAGCAG CACAACCCCG GGGCATGCAG CAGCCTCCAG CACAACCTCT CAGTTCATCT 
CAGCCTAGTC TCCGTGCTCA AGTGCCTCCT CCATTACTCT CCCCTCAGGT TCCAGTTTCA 
TTGCTGAAGT ATGCACCAAA CAACGGTGGC CTGAATCCGC TCTTTGGCCC TCAACAGGTA 
GCCATGCTGA ACCAGCTATC CCAACTAAAC CAGCTTTCTC AGATCTCCCA GTTACAGCGA 
TTGTTAGCGC AGCAGCAAAG GGCGCAGAGT CAGAGAAGCG TGCCTTCTGG GAACCGGCAG 
CAGCAAGACC AGCAGGGTCG ACCTCTTAGC GTGCAGCAGC AGATGATGCA ACAATCTCGT 
CAACTTGATC CAAACCTGTT GGTGAAGCAA CAAACTCCGC CATCTCAGCA ACAGTCACTC 
CATCAGCCAG CCATGAAGTC TTTCCTTGAA AATGTCATGC CCCACACTAC ACCTGAGCTG 
CAAAAAGGGC CATCACCAAT AAATGCTTTC AGCAACTTCC CTATAGGCTT GAACTCAAAC 
TTGAATGTAA ATATGGATAT GAACAGTATT AAAGAGCCAC AGTCAAGACT AAGGAAGTGG 
ACTACAGTGG ACAGCATTTC TGTGAACACA TCTTTGGATC AAAACTCCAG CAAACATGGT 
GCTATTTCAA GTGGTTTCAG GCTGGAAGAG TCTCCATTTG TTCCCTATGA CTTTATGAAC 
AGCAGTACTT CACCAGCCAG TCCTCCAGGT TCAATAGGAG ATGGCTGGCC ACGTGCCAAA 
TCGCCTAACG GCTCTAGCAG TGTTAATTGG CCACCAGAAT TTCGCCCTGG TGAACCATGG 
AAAGGTTATC CAAACATTGA CCCTGAAACT GACCCTTACG TCACTCCTGG CAGTGTCATA 
AACAATCTTT CAATTAATAC TGTGCGGGAA GTTGACCACC TCAGGGACAG GAACAGTGGG 
TCATCCTCAT CCTTGAACAC CACGCTGCCT TCAACTAGTG CCTGGTCATC CATTCGTGCC 
TCCAACTACA ACGCTCCCCT CAGCAGCACA GCACAAAGCA CTGCAGCCAG AAATAGTGAT 
TCCAAATTGA CATGGTCCCC TGGTTCAGTT ACAAACACCT CTCTGGCTCA TGAGCTGTGG 
AAGGTCCCTC TGCCACCTAA AAACATCACT GCTCCGTCCC GCCCACCTCC GGGACTGACT 
GGTCAGAAGC CACCCTTGTC TTCGTGGGAT AATTCTCCCC TTCGAGTAGG TGGAGGATGG 
GGAAATTCTG ACGCCAGGTA TACCCCAGGT TCCAGCTGGG GTGAGAGCAG CTCAGGGAGA 
ATAACAAATT GGCTTGTTCT AAAAAACCTT ACACCTCAGA TTGATGGCTC AACTCTGCGT 
ACTCTGTGCA TGCAGCATGG CCCACTGATA ACATTCCACC TGAACCTCCC ACATGGAAAC 
GCTTTGGTCC GTTACAGTTC AAAAGAAGAG GTAGTGAAGG CACAAAAGTC TCTGCACATG 
TGTGTACTGG GGAACACTAC TATTCTTGCT GAGTTTGCCA GTGAAGAGGA GATCAGTCGT 
TTCTTTGCAC AAAGTCAGTC TCTGACCCCT TCTCCCAGCT GGCAGTCTCT CGGGTCCAGC 
CAGAGCCGGC TGGGCTCCCT CGACTGTTCC CACTCATTCT CCAGCCGGAC CGATCTCAAT 
CACTGGAATG GTGCTGGGCT GTCGGGAACT AACTGTGGAG ACCTTCATGG CACTTCCCTC 
TGGGGGACCC CACATTATTC CACAAGCCTG TGGGGTCCTC CCAGCAGCAG CGACCCCCGG 
GGAATTAGCA GCCCATCCCC CATTAACGCT TTTCTTTCTG TTGACCACCT GGGTGGGGGT 
GGAGAGTCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_015469343.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324829.1 Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

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CloneID	OBa95212
Clone ID Related Accession (Same CDS sequence)	XM_015469343.1
Accession Version	XM_015469343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTT CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGGATGG CATTGTAGCA GATGAATCCC AAAACATGCA GTTTATGTCC 
AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT AGTGCACTAC CTAACCAGGC CCTGGGCTCC 
ATAGCAGGGC TGGGTATGCA AAACTTGAAT TCTGTTAGAC AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324829.1
CDS	153..5999
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQQQQQQPQPQQQQPQQPQQPQPPPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLSSQSESSALTNQQPQNNGEVQISKNQSDTNHNTSGSHYENSERGPVSSTSDSRTNCKNAVVNDSPEKEAWPSAPGSDPELVSECMDADSASSSESERPITIMASGSTGGENDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQASVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSPLGQMPNNQSINSKVGGASTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSNTGAWRVSTMNHPQIQAPSVVNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSSPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAVNSQSASWGNGANSGGSRRGWGTPAQNTGTNTPGVEWNKLPGNQHSSDSANGNGKKFTNGWKSTEEEDQGSAASQTNEQSSVWAKTGGTVESEGSTESTGRLEEKATGENQSRDRRKMDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACVDKTSPSSNDTSSVSGWGDPKPALRWGDSKGSSCQGGWEDDSAATGMVKSNQWGNCKEEKSAWNDSQKNKQGWGDGQKSNQGWSVSAGDNWGERSNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPNPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPMPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGSSRSDQQAQVHQLLPSAGTISNKEASSGSGWGEPWGETSTPATTVDNGTSAWGKPVDSGPSWGEPIAAASSTSTWGSSSVGPQTLNKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMDRSPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPSLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRQQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQSLHQPAMKSFLENVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNAPLSSTAQSTAARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSSWDNSPLRVGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPSWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_015469343.1
Organism	Bos taurus(cattle)
Definition	Bos taurus trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469343.1

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GGAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCCACAGCC AACAATCAGC AGCAGCAGCA GCAACAGCCG 
CAACCGCAGC AGCAGCAACC GCAGCAGCCG CAGCAGCCGC AGCCGCCACC ACAGGCCTTG 
CCTCGGTATC CCCGGGAAGT ACCACCTCGA TTTCGCCACC AGGAACATAA GCAGCTTCTA 
AAGAGGGGTC AGCATTTTCC CGTCATAGCA GCAAACCTGG GATCTGCTGT TAAAGTGTTA 
AGCAGCCAGT CAGAAAGCAG TGCTTTAACA AATCAACAGC CACAAAATAA CGGAGAGGTG 
CAGATCAGCA AAAACCAGTC AGATACAAAC CACAATACTT CAGGATCCCA TTATGAAAAT 
TCAGAGCGGG GACCTGTGTC GTCTACAAGT GACTCTCGTA CAAACTGTAA GAATGCTGTT 
GTGAACGACT CCCCTGAAAA AGAAGCATGG CCCTCAGCCC CTGGCAGTGA TCCGGAGTTG 
GTTTCAGAAT GTATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGCG ACCCATCACT 
ATCATGGCTT CAGGGAGCAC AGGTGGTGAA AATGATGGCC TTCGGAATAG CACTGGACTT 
GGATCCCAAA ACAAGTTTGT GGTTGGTAGC AGCAGCAATA ATGTGGGCCA CGGAAGTAGC 
ACTGGGCCGT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGC CTCTGTGGAT 
GCTCCTGAAA GCAAATCAGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT 
TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC 
TGGCCAGTAT TAGAGAACAA CGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT 
GGCATCAATA TTCAGTGCAG TCCCTTAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT 
AAAGTGGGTG GTGCTTCCAC CCATGGTACC TGGGGAAGCC TTCAGGAAAC TTGTGAATCT 
GAAGTAAGTG GTACACAGAA GGTTTCATTC AGTGGTCAAC CTCAAAATAT TACCACTGAA 
ATGACTGGAC CAAATAACAC TACTAACTTT ATGACCTCTA GTTTACCAAA CTCCGGTTCA 
GTACAGAATA ATGAACTGCC TAGTAATACA GGGGCCTGGC GTGTGAGCAC AATGAATCAT 
CCTCAGATAC AGGCTCCCTC GGTTGTAAAT GGCACTTCCC TTTCTCACCT TAGCAATGGA 
GAGTCAAAAA GTGGAGGCTC TTACGGTACT ACATGGGGTG CCTATGGTTC TAATTACTCT 
GGAGACAAAT GTTCAAGCCC TAATGGCCAA GCTAATGGTG ACACTGTGAA TGCAACTCTA 
ATGCAGCCTG GCGTAAACGG GCCTATGGGC ACTAACTTTC AAGTTAACAC AAACAAAGGA 
GGCGGCGTGT GGGAGTCTGG AGCAGTGAAT TCCCAGAGTG CATCCTGGGG AAATGGTGCA 
AATTCTGGAG GAAGTCGAAG AGGGTGGGGA ACTCCTGCAC AAAACACTGG CACTAATACA 
CCCGGCGTGG AGTGGAACAA ACTGCCTGGC AACCAGCATT CCAGTGACAG TGCAAATGGC 
AACGGTAAGA AGTTTACAAA CGGATGGAAG TCTACTGAGG AAGAGGATCA GGGTTCTGCC 
GCATCTCAGA CAAATGAGCA AAGCAGTGTG TGGGCCAAAA CAGGAGGTAC AGTGGAGAGT 
GAAGGTAGTA CAGAAAGCAC TGGACGCCTC GAGGAGAAAG CGACTGGGGA AAATCAGAGT 
AGGGACAGAA GGAAGATGGA TCAGCACACG TTACTCCAAA GCATTGTAAA CAGAACTGAC 
TTAGATCCAC GTGTCCTGTC CAACTCTGGC TGGGGACAGA CTCCTATTAA GCAGAATACT 
GCCTGGGATA CAGAAACATC ACCAAGAGGG GAGAGAAAGA CTGACAATGG GACAGAGGCC 
TGGGGAAGCT CTGCAACACA GACTTTTAAC TCAGGGGCGT GTGTAGATAA GACTAGCCCG 
AGTAGTAATG ATACCTCATC TGTATCGGGG TGGGGAGATC CCAAACCTGC TCTGCGGTGG 
GGAGATTCCA AAGGCTCAAG CTGCCAGGGG GGATGGGAAG ATGACTCTGC TGCTACAGGA 
ATGGTCAAGA GCAATCAGTG GGGGAACTGC AAAGAAGAGA AGTCCGCATG GAATGATTCG 
CAAAAGAACA AACAGGGATG GGGTGACGGA CAAAAGTCAA ACCAAGGGTG GTCCGTCTCT 
GCCGGTGACA ACTGGGGAGA AAGGAGTAAC CATTGGGGAG AGGCTAACAA GAAGTCTAGC 
TCAGGAGGTA GTGACAGTGA TAGGTCTGTT TCTGGTTGGA ATGAACTCGG TAAAACCAGT 
TCTTTTACAT GGGGAAATAA CATTAATCCA AATAATTCAT CAGGCTGGGA CGAATCTTCT 
AAACCGAATC CTTCCCAGGG ATGGGGAGAC CCTCCAAAGT CTAATCAGTC TCTAGGTTGG 
GGAGATTCAT CAAAGCCGGT CAGTTCTCCA GACTGGAACA AGCAACAAGA CATTGTCGGA 
TCTTGGGGCA TCCCGCCAGC GACAGGCAAA CCTCCTGGTA CAGGCTGGCT GGGAGGACCT 
ATGCCAGCCC CAGCGAAAGA GGAAGAACCC ACGGGCTGGG AGGAGCCATC CCCAGAATCC 
ATACGTCGCA AAATGGAGAT TGACGATGGG ACTTCAGCTT GGGGAGATCC CAGCAAATAC 
AATTACAAAA ATGTGAACAT GTGGAACAAA AACGTCCCAA ATGGCAGCAG TCGCTCAGAC 
CAGCAAGCAC AGGTACACCA GTTGCTACCG TCTGCAGGTA CCATCTCAAA CAAAGAGGCG 
AGCAGTGGCT CCGGCTGGGG TGAGCCCTGG GGGGAGACTT CAACTCCAGC CACAACTGTG 
GATAATGGTA CTTCAGCCTG GGGTAAACCC GTAGACAGTG GGCCTAGCTG GGGCGAACCC 
ATTGCTGCGG CATCCAGCAC ATCCACGTGG GGCTCCAGCT CTGTTGGTCC ACAAACATTA 
AACAAATCTG GGCCAAAATC TATGCAAGAC GGCTGGTGTG GTGATGATAT GCCCTTGCCT 
GGAAATCGCC CCACTGGCTG GGAAGAGGAA GAAGATGTAG AGATTGGAAT GTGGAATAGT 
AATTCATCTC AAGAGCTTAA TTCATCTTTA AATTGGCCAC CATATACAAA GAAAATGTCT 
TCGAAGGGTC TGAGTGGCAA AAAAAGGAGA AGGGAAAGGG GAATGATGAA AGGTGGAAAC 
AAACAAGAAG AAGCATGGAT AAATCCATTT GTTAAACAGT TTTCAAATAT CAGTTTTTCG 
AGAGACTCGC CAGAAGAAAA TGTACAGAGC AATAAGATGG ACCTTTCTGG AGGAATGTTA 
CAAGACAAAC GAATGGAGAT AGATAAACAT AGCCTAAATA TTGGTGATTA CAATCGAACG 
GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG ATTTCCAAAG AGTCTTCCAT GGACCGCAGT 
CCTTATTTTG ATAAGGATGG CATTGTAGCA GATGAATCCC AAAACATGCA GTTTATGTCC 
AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT AGTGCACTAC CTAACCAGGC CCTGGGCTCC 
ATAGCAGGGC TGGGTATGCA AAACTTGAAT TCTGTTAGAC AGAATGGCAA TCCCAGTATG 
TTTGGTGTTG GAAACACAGC AGCACAACCC CGGGGCATGC AGCAGCCTCC AGCACAACCT 
CTCAGTTCAT CTCAGCCTAG TCTCCGTGCT CAAGTGCCTC CTCCATTACT CTCCCCTCAG 
GTTCCAGTTT CATTGCTGAA GTATGCACCA AACAACGGTG GCCTGAATCC GCTCTTTGGC 
CCTCAACAGG TAGCCATGCT GAACCAGCTA TCCCAACTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC AGCAGCAAGA CCAGCAGGGT CGACCTCTTA GCGTGCAGCA GCAGATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AACAAACTCC GCCATCTCAG 
CAACAGTCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG AAAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACTACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGCCCT 
GGTGAACCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGCTCCC CTCAGCAGCA CAGCACAAAG CACTGCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCC CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TCTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTTCGTGGG ATAATTCTCC CCTTCGAGTA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGG TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATTGATGGC 
TCAACTCTGC GTACTCTGTG CATGCAGCAT GGCCCACTGA TAACATTCCA CCTGAACCTC 
CCACATGGAA ACGCTTTGGT CCGTTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGTCAG TCTCTGACCC CTTCTCCCAG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAT 
GGCACTTCCC TCTGGGGGAC CCCACATTAT TCCACAAGCC TGTGGGGTCC TCCCAGCAGC 
AGCGACCCCC GGGGAATTAG CAGCCCATCC CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNRC6A
Entrez Gene ID	541118
Full Name	trinucleotide repeat containing 6A
General protein information	Preferred Names trinucleotide repeat containing 6A Names trinucleotide repeat-containing gene 6A protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	TNRC6A	NP_055309.2
	TNRC6A	XP_001098013.2
Bos taurus (cattle)	TNRC6A	XP_005224864.1
Gallus gallus (chicken)	TNRC6A	XP_414871.4
Xenopus tropicalis (tropical clawed frog)	tnrc6a	XP_002932511.2
Canis lupus familiaris (dog)	TNRC6A	XP_005621456.1
Mus musculus (house mouse)	Tnrc6a	NP_659174.3
Rattus norvegicus (Norway rat)	Tnrc6a	NP_001101019.1
Pan troglodytes (chimpanzee)	TNRC6A	XP_510885.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNRC6A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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