TNRC6A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNRC6A
Entrez Gene ID	27327
Full Name	trinucleotide repeat containing 6A
Synonyms	CAGH26,GW1,GW182,TNRC6
General protein information	Preferred Names trinucleotide repeat containing 6A Names trinucleotide repeat-containing gene 6A protein CAG repeat protein 26 CTD-2540M10.1 EDIE EMSY interactor protein GW182 autoantigen glycine-tryptophan protein of 182 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.1
Summary	This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008].
Disorder MIM:	610739

mRNA and Protein(s)

mRNA	Protein	Name
NM_014494.3	NP_055309.2	trinucleotide repeat-containing gene 6A protein isoform 1
NM_014494.2	NP_055309.2	trinucleotide repeat-containing gene 6A protein isoform 1
XM_017023143.1	XP_016878632.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_017023150.1	XP_016878639.1	trinucleotide repeat-containing gene 6A protein isoform X9
NM_001351850.1	NP_001338779.1	trinucleotide repeat-containing gene 6A protein isoform 3
XM_017023144.2	XP_016878633.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_017023146.1	XP_016878635.1	trinucleotide repeat-containing gene 6A protein isoform X4
XM_024450232.1	XP_024306000.1	trinucleotide repeat-containing gene 6A protein isoform X6
NM_001330520.2	NP_001317449.1	trinucleotide repeat-containing gene 6A protein isoform 2
XM_017023145.1	XP_016878634.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_017023152.1	XP_016878641.1	trinucleotide repeat-containing gene 6A protein isoform X11
XM_017023151.1	XP_016878640.1	trinucleotide repeat-containing gene 6A protein isoform X10
XM_005255257.4	XP_005255314.1	trinucleotide repeat-containing gene 6A protein isoform X10
XM_017023154.1	XP_016878643.1	trinucleotide repeat-containing gene 6A protein isoform X11
NM_014494.4	NP_055309.2	trinucleotide repeat-containing gene 6A protein isoform 1
XM_017023144.1	XP_016878633.1	trinucleotide repeat-containing gene 6A protein isoform X2
XM_017023149.1	XP_016878638.1	trinucleotide repeat-containing gene 6A protein isoform X8
XM_017023150.2	XP_016878639.1	trinucleotide repeat-containing gene 6A protein isoform X7
NM_001330520.1	NP_001317449.1	trinucleotide repeat-containing gene 6A protein isoform 2
NM_001351850.2	NP_001338779.1	trinucleotide repeat-containing gene 6A protein isoform 3
XM_017023147.1	XP_016878636.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_017023148.1	XP_016878637.1	trinucleotide repeat-containing gene 6A protein isoform X7
XM_024450231.1	XP_024305999.1	trinucleotide repeat-containing gene 6A protein isoform X1
XM_017023145.2	XP_016878634.1	trinucleotide repeat-containing gene 6A protein isoform X3
XM_017023148.2	XP_016878637.1	trinucleotide repeat-containing gene 6A protein isoform X5
XM_024450233.1	XP_024306001.1	trinucleotide repeat-containing gene 6A protein isoform X8
XM_017023152.2	XP_016878641.1	trinucleotide repeat-containing gene 6A protein isoform X9
XM_017023153.1	XP_016878642.1	trinucleotide repeat-containing gene 6A protein isoform X10
NM_001330520.3	NP_001317449.1	trinucleotide repeat-containing gene 6A protein isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TNRC6A	NP_055309.2
	TNRC6A	XP_001098013.2
Bos taurus (cattle)	TNRC6A	XP_005224864.1
Gallus gallus (chicken)	TNRC6A	XP_414871.4
Xenopus tropicalis (tropical clawed frog)	tnrc6a	XP_002932511.2
Canis lupus familiaris (dog)	TNRC6A	XP_005621456.1
Mus musculus (house mouse)	Tnrc6a	NP_659174.3
Rattus norvegicus (Norway rat)	Tnrc6a	NP_001101019.1
Pan troglodytes (chimpanzee)	TNRC6A	XP_510885.3

Gene Ontology
Bibliography

Related articles in PubMed

Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry.
Cieplak-Rotowska MK, Tarnowski K, Rubin M, Fabian MR, Sonenberg N, Dadlez M, Niedzwiecka A
Journal of the American Society for Mass Spectrometry(2017 Oct)

Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.
Kourtidis A, Necela B, Lin WH, Lu R, Feathers RW, Asmann YW, Thompson EA, Anastasiadis PZ
The Journal of cell biology216(10)3073-3085(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In miRNA-mediated gene silencing, the physical interaction between human Argonaute (hAgo) and GW182 (hGW182) is essential for facilitating the downstream silencing of the targeted mRNA. hGW182 can recruit up to three copies of hAgo via its three GW motifs. This may explain the observed cooperativity in miRNA-mediated gene silencing.
Title: Multivalent Recruitment of Human Argonaute by GW182.

The results suggest that overexpressed Dcp1a and GW182 can form different cytoplasmic aggregates and play distinct biological roles in the miRNA pathway.
Title: Dcp1a and GW182 Induce Distinct Cellular Aggregates and Have Different Effects on microRNA Pathway.

that the interactions observed between TNRC6A and importin-alpha are conserved between mouse and human complexes. Our results highlight the ability of monopartite cNLS sequences to maximise contacts at the importin-alpha major binding site, as well as regions outside the main binding cavities
Title: Structural characterisation of TNRC6A nuclear localisation signal in complex with importin-alpha.

miRNAs, AGOs, GW182, the CCR4-NOT complex, and DDX6/Me31B repress and degrade polyadenylated mRNA targets that are translated via scanning-independent mechanisms in both human and Drosophila melanogaster cells
Title: miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning.

The results indicate that TNRC6A subcellular localization is substantially controlled by the interaction with Argonaute proteins.
Title: Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein.

The following TNRC6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNRC6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu85982	XM_017023152.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85983	XM_017023154.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu49123	XM_017023153.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu49123	XM_005255257.4 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu06415	NM_014494.4 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu49118	NM_001330520.3 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85977	NM_001351850.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85973	XM_017023143.1 Latest version!	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85973	XM_024450231.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85974	XM_017023144.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85975	XM_017023145.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85976	XM_017023146.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85977	XM_017023147.1 Latest version!	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85978	XM_017023148.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85979	XM_024450232.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85979	XM_017023149.1 Latest version!	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85980	XM_017023150.2 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85981	XM_017023151.1 Latest version!	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85981	XM_024450233.1 Latest version!	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu06415	NM_014494.2	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu06415	NM_014494.3	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu85974	XM_017023144.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85980	XM_017023150.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu49118	NM_001330520.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85977	NM_001351850.1	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85975	XM_017023145.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85978	XM_017023148.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85982	XM_017023152.1	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu49118	NM_001330520.2	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNRC6A ( XM_017023152.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878641.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu85982
Clone ID Related Accession (Same CDS sequence)	XM_017023152.1 , XM_017023152.2
Accession Version	XM_017023152.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG ATATAAACCA CAGTACTTCA GGATCCCATT ATGAAAATTC CCAGCGGGGA 
CCTGTGTCTT CTACAAGTGA TTCTAGCACA AACTGTAAGA ATGCTGTTGT AAGTGACTTG 
TCGGAAAAAG AAGCATGGCC CTCAGCCCCT GGCAGTGATC CGGAGTTGGC TTCAGAATGT 
ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878641.1
CDS	30..5519
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023152.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023152.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG ATATAAACCA CAGTACTTCA GGATCCCATT ATGAAAATTC CCAGCGGGGA 
CCTGTGTCTT CTACAAGTGA TTCTAGCACA AACTGTAAGA ATGCTGTTGT AAGTGACTTG 
TCGGAAAAAG AAGCATGGCC CTCAGCCCCT GGCAGTGATC CGGAGTTGGC TTCAGAATGT 
ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023152.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878641.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X9, mRNA.

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CloneID	OHu85982
Clone ID Related Accession (Same CDS sequence)	XM_017023152.1 , XM_017023152.2
Accession Version	XM_017023152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG ATATAAACCA CAGTACTTCA GGATCCCATT ATGAAAATTC CCAGCGGGGA 
CCTGTGTCTT CTACAAGTGA TTCTAGCACA AACTGTAAGA ATGCTGTTGT AAGTGACTTG 
TCGGAAAAAG AAGCATGGCC CTCAGCCCCT GGCAGTGATC CGGAGTTGGC TTCAGAATGT 
ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878641.1
CDS	275..5764
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023152.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023152.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG ATATAAACCA CAGTACTTCA GGATCCCATT ATGAAAATTC CCAGCGGGGA 
CCTGTGTCTT CTACAAGTGA TTCTAGCACA AACTGTAAGA ATGCTGTTGT AAGTGACTTG 
TCGGAAAAAG AAGCATGGCC CTCAGCCCCT GGCAGTGATC CGGAGTTGGC TTCAGAATGT 
ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023154.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878643.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X12, mRNA.

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CloneID	OHu85983
Clone ID Related Accession (Same CDS sequence)	XM_017023154.1
Accession Version	XM_017023154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GAATGATGAA AGGTGGAAAC AAACAAGAAG AAGCGTGGAT AAATCCATTT 
GTTAAACAGT TTTCAAACAT CAGTTTTTCG AGAGACTCAC CAGAGGAAAA TGTACAAAGC 
AATAAGATGG ACCTTTCTGG AGGAATGTTA CAAGACAAAC GAATGGAGAT AGATAAACAT 
AGCCTAAATA TTGGTGATTA CAATCGAACG GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG 
ATTTCCAAAG AGTCTTCCAT GGAGCGCAAT CCTTATTTTG ATAAGGATGG CATTGTAGCA 
GATGAATCCC AAAACATGCA GTTTATGTCC AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT 
AGTGCACTAC CTAACCAGGC CCTTGGCTCC ATAGCAGGGC TGGGTATGCA AAACTTGAAT 
TCTGTTAGAC AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTTAGTTCAT CTCAGCCTAA TCTCCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC ACTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAGCTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGC GATTGTTAGC GCAGCAGCAA 
AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT GGGAACCGGC CGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GTGTGCAGCA GCAAATGATG CAACAATCTC GTCAACTTGA TCCAAACCTG 
TTGGTGAAGC AGCAGACTCC ACCATCTCAG CAGCAGCCAC TCCATCAGCC AGCCATGAAG 
TCTTTCCTTG ACAATGTCAT GCCCCACACT ACACCTGAGC TGCAAAAAGG GCCATCACCA 
ATAAATGCTT TCAGCAACTT CCCTATAGGC TTGAACTCAA ACTTGAATGT AAATATGGAT 
ATGAACAGTA TTAAAGAGCC ACAGTCAAGA CTAAGGAAGT GGACGACAGT GGACAGCATT 
TCTGTGAACA CATCTTTGGA TCAAAACTCC AGCAAACATG GTGCTATTTC AAGTGGTTTC 
AGGCTGGAAG AGTCTCCATT TGTTCCCTAT GACTTTATGA ACAGCAGTAC TTCACCAGCC 
AGTCCTCCAG GTTCAATAGG AGATGGCTGG CCACGTGCCA AATCGCCTAA CGGCTCTAGC 
AGTGTTAATT GGCCACCAGA ATTTCGTCCT GGTGAGCCAT GGAAAGGTTA TCCAAACATT 
GACCCTGAAA CTGACCCTTA CGTCACTCCT GGCAGTGTCA TAAACAATCT TTCAATTAAT 
ACTGTGCGGG AAGTTGACCA CCTCAGGGAC AGGAACAGTG GGTCATCCTC ATCCTTGAAC 
ACCACGCTGC CTTCAACTAG TGCCTGGTCA TCCATTCGTG CCTCCAACTA CAACGTTCCC 
CTCAGCAGTA CAGCACAAAG CACTTCAGCC AGAAATAGTG ATTCCAAATT GACATGGTCT 
CCTGGTTCAG TTACAAACAC CTCTCTGGCT CATGAGCTGT GGAAGGTCCC TTTGCCACCT 
AAAAACATCA CTGCTCCGTC CCGCCCACCT CCGGGACTGA CTGGTCAGAA GCCACCCTTG 
TCTACGTGGG ATAATTCTCC CCTTCGTATA GGTGGAGGAT GGGGAAATTC TGACGCCAGA 
TATACCCCAG GTTCCAGCTG GGGTGAGAGC AGCTCAGGGA GAATAACAAA TTGGCTTGTT 
CTAAAAAACC TTACACCTCA GATCGATGGC TCAACTCTGC GCACTCTGTG CATGCAGCAC 
GGCCCGCTGA TCACATTCCA CCTGAACCTC CCTCACGGAA ATGCTCTGGT CCGCTACAGT 
TCAAAAGAAG AGGTAGTGAA GGCACAAAAG TCTCTGCACA TGTGTGTACT GGGGAACACT 
ACTATTCTTG CTGAGTTTGC CAGTGAAGAG GAGATCAGTC GTTTCTTTGC ACAAAGCCAG 
TCTCTGACCC CTTCTCCCGG CTGGCAGTCT CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC 
CTCGACTGTT CCCACTCATT CTCCAGCCGG ACCGATCTCA ATCACTGGAA TGGTGCTGGG 
CTGTCGGGAA CTAACTGTGG AGACCTTCAC GGCACTTCAC TCTGGGGGAC CCCGCATTAT 
TCCACAAGCC TGTGGGGTCC CCCAAGCAGC AGCGACCCCC GAGGAATTAG CAGCCCATCT 
CCCATTAACG CTTTTCTTTC TGTTGACCAC CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878643.1
CDS	765..5861
Translation	MDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023154.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023154.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GAATGATGAA AGGTGGAAAC AAACAAGAAG AAGCGTGGAT AAATCCATTT 
GTTAAACAGT TTTCAAACAT CAGTTTTTCG AGAGACTCAC CAGAGGAAAA TGTACAAAGC 
AATAAGATGG ACCTTTCTGG AGGAATGTTA CAAGACAAAC GAATGGAGAT AGATAAACAT 
AGCCTAAATA TTGGTGATTA CAATCGAACG GTCGGGAAAG GCCCTGGTTC TCGGCCTCAG 
ATTTCCAAAG AGTCTTCCAT GGAGCGCAAT CCTTATTTTG ATAAGGATGG CATTGTAGCA 
GATGAATCCC AAAACATGCA GTTTATGTCC AGTCAAAGCA TGAAGCTTCC CCCTTCAAAT 
AGTGCACTAC CTAACCAGGC CCTTGGCTCC ATAGCAGGGC TGGGTATGCA AAACTTGAAT 
TCTGTTAGAC AGAATGGCAA TCCCAGTATG TTTGGTGTTG GAAACACAGC AGCACAACCC 
CGGGGCATGC AGCAGCCTCC AGCACAACCT CTTAGTTCAT CTCAGCCTAA TCTCCGTGCT 
CAAGTGCCTC CTCCATTACT CTCCCCTCAG GTTCCAGTTT CATTGCTGAA GTATGCACCA 
AACAACGGTG GCCTGAATCC ACTCTTTGGC CCTCAACAGG TAGCCATGCT GAACCAGCTA 
TCCCAGCTAA ACCAGCTTTC TCAGATCTCC CAGTTACAGC GATTGTTAGC GCAGCAGCAA 
AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT GGGAACCGGC CGCAGCAAGA CCAGCAGGGT 
CGACCTCTTA GTGTGCAGCA GCAAATGATG CAACAATCTC GTCAACTTGA TCCAAACCTG 
TTGGTGAAGC AGCAGACTCC ACCATCTCAG CAGCAGCCAC TCCATCAGCC AGCCATGAAG 
TCTTTCCTTG ACAATGTCAT GCCCCACACT ACACCTGAGC TGCAAAAAGG GCCATCACCA 
ATAAATGCTT TCAGCAACTT CCCTATAGGC TTGAACTCAA ACTTGAATGT AAATATGGAT 
ATGAACAGTA TTAAAGAGCC ACAGTCAAGA CTAAGGAAGT GGACGACAGT GGACAGCATT 
TCTGTGAACA CATCTTTGGA TCAAAACTCC AGCAAACATG GTGCTATTTC AAGTGGTTTC 
AGGCTGGAAG AGTCTCCATT TGTTCCCTAT GACTTTATGA ACAGCAGTAC TTCACCAGCC 
AGTCCTCCAG GTTCAATAGG AGATGGCTGG CCACGTGCCA AATCGCCTAA CGGCTCTAGC 
AGTGTTAATT GGCCACCAGA ATTTCGTCCT GGTGAGCCAT GGAAAGGTTA TCCAAACATT 
GACCCTGAAA CTGACCCTTA CGTCACTCCT GGCAGTGTCA TAAACAATCT TTCAATTAAT 
ACTGTGCGGG AAGTTGACCA CCTCAGGGAC AGGAACAGTG GGTCATCCTC ATCCTTGAAC 
ACCACGCTGC CTTCAACTAG TGCCTGGTCA TCCATTCGTG CCTCCAACTA CAACGTTCCC 
CTCAGCAGTA CAGCACAAAG CACTTCAGCC AGAAATAGTG ATTCCAAATT GACATGGTCT 
CCTGGTTCAG TTACAAACAC CTCTCTGGCT CATGAGCTGT GGAAGGTCCC TTTGCCACCT 
AAAAACATCA CTGCTCCGTC CCGCCCACCT CCGGGACTGA CTGGTCAGAA GCCACCCTTG 
TCTACGTGGG ATAATTCTCC CCTTCGTATA GGTGGAGGAT GGGGAAATTC TGACGCCAGA 
TATACCCCAG GTTCCAGCTG GGGTGAGAGC AGCTCAGGGA GAATAACAAA TTGGCTTGTT 
CTAAAAAACC TTACACCTCA GATCGATGGC TCAACTCTGC GCACTCTGTG CATGCAGCAC 
GGCCCGCTGA TCACATTCCA CCTGAACCTC CCTCACGGAA ATGCTCTGGT CCGCTACAGT 
TCAAAAGAAG AGGTAGTGAA GGCACAAAAG TCTCTGCACA TGTGTGTACT GGGGAACACT 
ACTATTCTTG CTGAGTTTGC CAGTGAAGAG GAGATCAGTC GTTTCTTTGC ACAAAGCCAG 
TCTCTGACCC CTTCTCCCGG CTGGCAGTCT CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC 
CTCGACTGTT CCCACTCATT CTCCAGCCGG ACCGATCTCA ATCACTGGAA TGGTGCTGGG 
CTGTCGGGAA CTAACTGTGG AGACCTTCAC GGCACTTCAC TCTGGGGGAC CCCGCATTAT 
TCCACAAGCC TGTGGGGTCC CCCAAGCAGC AGCGACCCCC GAGGAATTAG CAGCCCATCT 
CCCATTAACG CTTTTCTTTC TGTTGACCAC CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_005255257.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255314.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X11, mRNA.

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CloneID	OHu49123
Clone ID Related Accession (Same CDS sequence)	XM_005255257.4 , XM_017023153.1
Accession Version	XM_005255257.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255257.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255314.1
CDS	841..5970
Translation	MDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_005255257.4
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255257.4

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023153.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878642.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X10, mRNA.

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CloneID	OHu49123
Clone ID Related Accession (Same CDS sequence)	XM_005255257.4 , XM_017023153.1
Accession Version	XM_017023153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878642.1
CDS	841..5970
Translation	MDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023153.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023153.1

ORF Insert Sequence:

ATGGATGCTG ATTCTGCCTC CAGTTCTGAA TCAGAGAGAA ACATCACTAT CATGGCTTCA 
GGGAACACAG GTGGTGAAAA AGATGGCCTT CGGAATAGCA CTGGACTTGG TTCCCAAAAC 
AAGTTTGTAG TTGGTAGCAG CAGCAATAAT GTGGGCCATG GAAGTAGTAC TGGGCCATGG 
GGTTTTTCCC ATGGAGCCAT AATAAGCACA TGTCAGGTCT CTGTGGATGC TCCTGAAAGC 
AAATCTGAAA GTAGCAACAA TAGAATGAAT GCTTGGGGCA CTGTAAGTTC TTCATCAAAT 
GGAGGGTTAA ATCCAAGCAC TTTGAATTCA GCTAGCAACC ATGGTGCCTG GCCAGTATTA 
GAGAACAATG GACTTGCCCT AAAAGGGCCT GTAGGGAGTG GTAGTTCTGG CATTAATATT 
CAGTGCAGTA CTATAGGCCA GATGCCTAAC AATCAGAGTA TTAACTCTAA AGTGAGTGGT 
GGTTCTACCC ATGGTACCTG GGGAAGCCTT CAGGAAACTT GTGAATCTGA AGTAAGTGGT 
ACACAGAAGG TTTCATTCAG TGGTCAACCT CAAAATATTA CCACTGAAAT GACTGGACCA 
AATAACACTA CTAACTTTAT GACCTCTAGT TTACCAAACT CCGGTTCAGT GCAGAATAAT 
GAGCTGCCTA GTAGTAACAC AGGGGCCTGG CGTGTGAGCA CAATGAATCA TCCTCAGATG 
CAGGCTCCAT CAGGTATGAA TGGCACTTCC CTTTCTCACC TTAGCAATGG AGAGTCAAAA 
AGTGGAGGCT CTTATGGTAC TACATGGGGT GCCTATGGTT CTAATTACTC TGGAGACAAA 
TGTTCAGGCC CTAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT 
GGCGTAAATG GTCCTATGGG CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG 
TGGGAATCTG GTGCAGCAAA CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT 
TCTGGAGGAA GTCGAAGAGG ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC 
AGCGTTGAGT GGAACAAACT GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT 
GGTAAGACGT TTACAAATGG ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA 
TCTCAGACAA ATGAGCAAAG CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT 
GGTAGTACAG AAAGCACTGG ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA 
GACAGAAGAA AAATTGATCA GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA 
GATCCACGTG TCCTGTCCAA CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC 
TGGGATACAG AAACATCACC TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG 
GGAAGCTCTG CAACACAGAC TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT 
GGTAATGATA CCTCATCTGT ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA 
GATTCCAAAG GCTCAAACTG CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG 
GTCAAGAGCA ATCAGTGGGG GAATTGCAAA GAGGAGAAGG CTGCATGGAA TGACTCGCAA 
AAGAATAAAC AGGGATGGGG TGATGGACAA AAATCAAGCC AAGGGTGGTC TGTTTCTGCC 
AGTGATAACT GGGGAGAAAC TTCAAGGAAT AACCATTGGG GTGAGGCCAA TAAGAAATCC 
AGCTCAGGAG GTAGTGACAG TGACAGGTCC GTTTCCGGTT GGAACGAACT TGGTAAAACT 
AGTTCTTTCA CTTGGGGAAA CAACATAAAT CCAAATAATT CATCAGGATG GGATGAATCT 
TCTAAACCTA CTCCTTCCCA GGGATGGGGA GACCCTCCAA AGTCTAATCA GTCTCTAGGT 
TGGGGAGATT CGTCAAAGCC AGTCAGCTCT CCAGACTGGA ACAAGCAACA AGACATTGTT 
GGATCTTGGG GAATCCCACC AGCTACAGGC AAACCTCCTG GTACAGGCTG GCTGGGGGGA 
CCTATACCAG CCCCAGCAAA AGAAGAAGAA CCCACAGGCT GGGAGGAACC ATCCCCAGAA 
TCTATACGTC GCAAAATGGA GATTGATGAT GGAACTTCAG CTTGGGGAGA TCCAAGCAAA 
TACAACTACA AAAATGTGAA CATGTGGAAC AAAAACGTCC CAAATGGCAA CAGCCGTTCA 
GACCAGCAAG CACAGGTACA TCAGCTGCTA ACGCCTGCAA GTGCCATCTC AAACAAAGAG 
GCAAGCAGTG GCTCTGGCTG GGGTGAGCCC TGGGGGGAGC CTTCTACTCC AGCCACAACT 
GTGGATAATG GTACTTCAGC ATGGGGTAAG CCCATAGACA GTGGTCCCAG CTGGGGGGAA 
CCCATTGCTG CGGCATCCAG CACATCCACG TGGGGCTCCA GCTCTGTTGG TCCACAAGCA 
TTAAGCAAAT CTGGGCCAAA ATCTATGCAA GATGGCTGGT GTGGTGATGA TATGCCATTG 
CCTGGAAATC GCCCCACTGG CTGGGAAGAG GAAGAGGATG TGGAGATTGG AATGTGGAAT 
AGTAATTCAT CTCAAGAGCT TAACTCATCT TTAAATTGGC CACCATATAC AAAGAAAATG 
TCATCGAAGG GTCTGAGTGG CAAAAAAAGG AGAAGGGAAA GGGGAATGAT GAAAGGTGGA 
AACAAACAAG AAGAAGCGTG GATAAATCCA TTTGTTAAAC AGTTTTCAAA CATCAGTTTT 
TCGAGAGACT CACCAGAGGA AAATGTACAA AGCAATAAGA TGGACCTTTC TGGAGGAATG 
TTACAAGACA AACGAATGGA GATAGATAAA CATAGCCTAA ATATTGGTGA TTACAATCGA 
ACGGTCGGGA AAGGCCCTGG TTCTCGGCCT CAGATTTCCA AAGAGTCTTC CATGGAGCGC 
AATCCTTATT TTGATAAGGA TGGCATTGTA GCAGATGAAT CCCAAAACAT GCAGTTTATG 
TCCAGTCAAA GCATGAAGCT TCCCCCTTCA AATAGTGCAC TACCTAACCA GGCCCTTGGC 
TCCATAGCAG GGCTGGGTAT GCAAAACTTG AATTCTGTTA GACAGAATGG CAATCCCAGT 
ATGTTTGGTG TTGGAAACAC AGCAGCACAA CCCCGGGGCA TGCAGCAGCC TCCAGCACAA 
CCTCTTAGTT CATCTCAGCC TAATCTCCGT GCTCAAGTGC CTCCTCCATT ACTCTCCCCT 
CAGGTTCCAG TTTCATTGCT GAAGTATGCA CCAAACAACG GTGGCCTGAA TCCACTCTTT 
GGCCCTCAAC AGGTAGCCAT GCTGAACCAG CTATCCCAGC TAAACCAGCT TTCTCAGATC 
TCCCAGTTAC AGCGATTGTT AGCGCAGCAG CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT 
TCTGGGAACC GGCCGCAGCA AGACCAGCAG GGTCGACCTC TTAGTGTGCA GCAGCAAATG 
ATGCAACAAT CTCGTCAACT TGATCCAAAC CTGTTGGTGA AGCAGCAGAC TCCACCATCT 
CAGCAGCAGC CACTCCATCA GCCAGCCATG AAGTCTTTCC TTGACAATGT CATGCCCCAC 
ACTACACCTG AGCTGCAAAA AGGGCCATCA CCAATAAATG CTTTCAGCAA CTTCCCTATA 
GGCTTGAACT CAAACTTGAA TGTAAATATG GATATGAACA GTATTAAAGA GCCACAGTCA 
AGACTAAGGA AGTGGACGAC AGTGGACAGC ATTTCTGTGA ACACATCTTT GGATCAAAAC 
TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC 
TATGACTTTA TGAACAGCAG TACTTCACCA GCCAGTCCTC CAGGTTCAAT AGGAGATGGC 
TGGCCACGTG CCAAATCGCC TAACGGCTCT AGCAGTGTTA ATTGGCCACC AGAATTTCGT 
CCTGGTGAGC CATGGAAAGG TTATCCAAAC ATTGACCCTG AAACTGACCC TTACGTCACT 
CCTGGCAGTG TCATAAACAA TCTTTCAATT AATACTGTGC GGGAAGTTGA CCACCTCAGG 
GACAGGAACA GTGGGTCATC CTCATCCTTG AACACCACGC TGCCTTCAAC TAGTGCCTGG 
TCATCCATTC GTGCCTCCAA CTACAACGTT CCCCTCAGCA GTACAGCACA AAGCACTTCA 
GCCAGAAATA GTGATTCCAA ATTGACATGG TCTCCTGGTT CAGTTACAAA CACCTCTCTG 
GCTCATGAGC TGTGGAAGGT CCCTTTGCCA CCTAAAAACA TCACTGCTCC GTCCCGCCCA 
CCTCCGGGAC TGACTGGTCA GAAGCCACCC TTGTCTACGT GGGATAATTC TCCCCTTCGT 
ATAGGTGGAG GATGGGGAAA TTCTGACGCC AGATATACCC CAGGTTCCAG CTGGGGTGAG 
AGCAGCTCAG GGAGAATAAC AAATTGGCTT GTTCTAAAAA ACCTTACACC TCAGATCGAT 
GGCTCAACTC TGCGCACTCT GTGCATGCAG CACGGCCCGC TGATCACATT CCACCTGAAC 
CTCCCTCACG GAAATGCTCT GGTCCGCTAC AGTTCAAAAG AAGAGGTAGT GAAGGCACAA 
AAGTCTCTGC ACATGTGTGT ACTGGGGAAC ACTACTATTC TTGCTGAGTT TGCCAGTGAA 
GAGGAGATCA GTCGTTTCTT TGCACAAAGC CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG 
TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC TCCCTCGACT GTTCCCACTC ATTCTCCAGC 
CGGACCGATC TCAATCACTG GAATGGTGCT GGGCTGTCGG GAACTAACTG TGGAGACCTT 
CACGGCACTT CACTCTGGGG GACCCCGCAT TATTCCACAA GCCTGTGGGG TCCCCCAAGC 
AGCAGCGACC CCCGAGGAAT TAGCAGCCCA TCTCCCATTA ACGCTTTTCT TTCTGTTGAC 
CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_014494.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055309.2 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.

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CloneID	OHu06415
Clone ID Related Accession (Same CDS sequence)	NM_014494.3 , NM_014494.2 , NM_014494.4
Accession Version	NM_014494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002565.1, BE158958.1, AJ492221.1, AC008731.8 and BU743266.1. On Jun 2, 2017 this sequence version replaced NM_014494.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055309.2
CDS	172..6060
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_014494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014494.3

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_014494.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055309.2 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.

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CloneID	OHu06415
Clone ID Related Accession (Same CDS sequence)	NM_014494.3 , NM_014494.2 , NM_014494.4
Accession Version	NM_014494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-15
Organism	Homo sapiens(Human)
Product	trinucleotide repeat-containing gene 6A protein isoform 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE158958.1, AJ492221.1, AC008731.8 and BU743266.1. On or before Oct 27, 2006 this sequence version replaced NM_020847.1, NM_014494.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055309.2
CDS	115..6003
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_014494.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014494.2

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_014494.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055309.2 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 1, mRNA.

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CloneID	OHu06415
Clone ID Related Accession (Same CDS sequence)	NM_014494.3 , NM_014494.2 , NM_014494.4
Accession Version	NM_014494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002565.1, BE158958.1, AJ492221.1, AC008731.8 and BU743266.1. On Nov 22, 2018 this sequence version replaced NM_014494.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000395799.8/ ENSP00000379144.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055309.2
CDS	192..6080
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_014494.4
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014494.4

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGGAT 
GGCATTGTAG CAGATGAATC CCAAAACATG CAGTTTATGT CCAGTCAAAG CATGAAGCTT 
CCCCCTTCAA ATAGTGCACT ACCTAACCAG GCCCTTGGCT CCATAGCAGG GCTGGGTATG 
CAAAACTTGA ATTCTGTTAG ACAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_001330520.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317449.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.

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CloneID	OHu49118
Clone ID Related Accession (Same CDS sequence)	NM_001330520.2 , NM_001330520.1 , NM_001330520.3
Accession Version	NM_001330520.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002565.1, AC008731.8 and BU743266.1. On Jun 2, 2017 this sequence version replaced NM_001330520.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.53998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317449.1
CDS	172..5913
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_001330520.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330520.2

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_001330520.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317449.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 2, mRNA.

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CloneID	OHu49118
Clone ID Related Accession (Same CDS sequence)	NM_001330520.2 , NM_001330520.1 , NM_001330520.3
Accession Version	NM_001330520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-23
Organism	Homo sapiens(Human)
Product	trinucleotide repeat-containing gene 6A protein isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002565.1, AC008731.8 and BU743266.1. On Aug 26, 2016 this sequence version replaced XM_005255254.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.53998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317449.1
CDS	115..5856
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_001330520.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330520.1

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_001330520.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317449.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.

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CloneID	OHu49118
Clone ID Related Accession (Same CDS sequence)	NM_001330520.2 , NM_001330520.1 , NM_001330520.3
Accession Version	NM_001330520.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002565.1, AC008731.8 and BU743266.1. On May 31, 2019 this sequence version replaced NM_001330520.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.53998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317449.1
CDS	192..5933
Translation	MRELEAKATKDVERNLSRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_001330520.3
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330520.3

ORF Insert Sequence:

ATGAGAGAAT TGGAAGCTAA AGCTACCAAA GACGTAGAAA GAAATCTTAG CAGGGATTTA 
GTGCAAGAAG AAGAACAGTT GATGGAAGAA AAGAAAAAGA AAAAAGACGA CAAGAAAAAG 
AAGGAAGCTG CTCAAAAGAA GGCCACTGAA CAAAAAATCA AAGTGCCAGA ACAGATAAAG 
CCCAGTGTAA GCCAGCCTCA GCCTGCCAAC TCTAATAACG GCACTTCCAC AGCAACCAGC 
ACTAATAATA ATGCCAAGCG AGCTACAGCC AACAATCAGC AGCCACAGCA GCAGCAGCAA 
CAGCAGCAGC CGCAGCAGCA GCAGCCACAG CAGCAGCCAC AGCCGCAGCC GCAGCAGCAG 
CAGCCACAGC AGCAGCCACA GGCCTTGCCT CGGTATCCTC GTGAAGTACC TCCACGATTT 
CGCCACCAGG AACACAAACA GCTTCTAAAG AGGGGTCAGC ATTTTCCTGT TATAGCAGCA 
AACCTTGGAT CTGCTGTTAA GGTGTTAAAC AGCCAGTCAG AAAGCAGTGC TTTAACAAAT 
CAACAGCCAC AAAATAACGG AGAGGTGCAG AACAGCAAAA ACCAGTCAGA TATAAACCAC 
AGTACTTCAG GATCCCATTA TGAAAATTCC CAGCGGGGAC CTGTGTCTTC TACAAGTGAT 
TCTAGCACAA ACTGTAAGAA TGCTGTTGTA AGTGACTTGT CGGAAAAAGA AGCATGGCCC 
TCAGCCCCTG GCAGTGATCC GGAGTTGGCT TCAGAATGTA TGGATGCTGA TTCTGCCTCC 
AGTTCTGAAT CAGAGAGAAA CATCACTATC ATGGCTTCAG GGAACACAGG TGGTGAAAAA 
GATGGCCTTC GGAATAGCAC TGGACTTGGT TCCCAAAACA AGTTTGTAGT TGGTAGCAGC 
AGCAATAATG TGGGCCATGG AAGTAGTACT GGGCCATGGG GTTTTTCCCA TGGAGCCATA 
ATAAGCACAT GTCAGGTCTC TGTGGATGCT CCTGAAAGCA AATCTGAAAG TAGCAACAAT 
AGAATGAATG CTTGGGGCAC TGTAAGTTCT TCATCAAATG GAGGGTTAAA TCCAAGCACT 
TTGAATTCAG CTAGCAACCA TGGTGCCTGG CCAGTATTAG AGAACAATGG ACTTGCCCTA 
AAAGGGCCTG TAGGGAGTGG TAGTTCTGGC ATTAATATTC AGTGCAGTAC TATAGGCCAG 
ATGCCTAACA ATCAGAGTAT TAACTCTAAA GTGAGTGGTG GTTCTACCCA TGGTACCTGG 
GGAAGCCTTC AGGAAACTTG TGAATCTGAA GTAAGTGGTA CACAGAAGGT TTCATTCAGT 
GGTCAACCTC AAAATATTAC CACTGAAATG ACTGGACCAA ATAACACTAC TAACTTTATG 
ACCTCTAGTT TACCAAACTC CGGTTCAGTG CAGAATAATG AGCTGCCTAG TAGTAACACA 
GGGGCCTGGC GTGTGAGCAC AATGAATCAT CCTCAGATGC AGGCTCCATC AGGTATGAAT 
GGCACTTCCC TTTCTCACCT TAGCAATGGA GAGTCAAAAA GTGGAGGCTC TTATGGTACT 
ACATGGGGTG CCTATGGTTC TAATTACTCT GGAGACAAAT GTTCAGGCCC TAATGGCCAA 
GCTAATGGTG ACACTGTGAA TGCAACTCTA ATGCAGCCTG GCGTAAATGG TCCTATGGGC 
ACTAACTTTC AAGTTAACAC AAACAAAGGA GGTGGTGTGT GGGAATCTGG TGCAGCAAAC 
TCCCAGAGTA CATCATGGGG AAGTGGAAAT GGCGCAAATT CTGGAGGAAG TCGAAGAGGA 
TGGGGAACCC CTGCACAAAA CACTGGCACT AATTTACCCA GCGTTGAGTG GAACAAACTG 
CCTAGCAATC AGCATTCCAA TGATAGTGCA AATGGCAATG GTAAGACGTT TACAAATGGA 
TGGAAATCTA CTGAGGAAGA GGATCAGGGT TCTGCCACAT CTCAGACAAA TGAGCAAAGC 
AGTGTGTGGG CCAAAACAGG AGGTACAGTG GAGAGCGATG GTAGTACAGA AAGCACTGGA 
CGCCTTGAGG AAAAAGGAAC TGGGGAAAGT CAGAGTAGAG ACAGAAGAAA AATTGATCAG 
CACACATTAC TCCAAAGCAT TGTAAACAGA ACTGACTTAG ATCCACGTGT CCTGTCCAAC 
TCTGGTTGGG GACAGACTCC TATTAAGCAG AATACTGCCT GGGATACAGA AACATCACCT 
AGAGGGGAAC GAAAGACTGA CAATGGGACA GAGGCCTGGG GAAGCTCTGC AACACAGACT 
TTTAACTCAG GGGCATGTAT AGATAAGACT AGCCCTAATG GTAATGATAC CTCATCTGTA 
TCAGGGTGGG GCGATCCCAA ACCTGCTCTG AGGTGGGGAG ATTCCAAAGG CTCAAACTGC 
CAGGGGGGGT GGGAAGATGA TTCTGCTGCT ACAGGAATGG TCAAGAGCAA TCAGTGGGGG 
AATTGCAAAG AGGAGAAGGC TGCATGGAAT GACTCGCAAA AGAATAAACA GGGATGGGGT 
GATGGACAAA AATCAAGCCA AGGGTGGTCT GTTTCTGCCA GTGATAACTG GGGAGAAACT 
TCAAGGAATA ACCATTGGGG TGAGGCCAAT AAGAAATCCA GCTCAGGAGG TAGTGACAGT 
GACAGGTCCG TTTCCGGTTG GAACGAACTT GGTAAAACTA GTTCTTTCAC TTGGGGAAAC 
AACATAAATC CAAATAATTC ATCAGGATGG GATGAATCTT CTAAACCTAC TCCTTCCCAG 
GGATGGGGAG ACCCTCCAAA GTCTAATCAG TCTCTAGGTT GGGGAGATTC GTCAAAGCCA 
GTCAGCTCTC CAGACTGGAA CAAGCAACAA GACATTGTTG GATCTTGGGG AATCCCACCA 
GCTACAGGCA AACCTCCTGG TACAGGCTGG CTGGGGGGAC CTATACCAGC CCCAGCAAAA 
GAAGAAGAAC CCACAGGCTG GGAGGAACCA TCCCCAGAAT CTATACGTCG CAAAATGGAG 
ATTGATGATG GAACTTCAGC TTGGGGAGAT CCAAGCAAAT ACAACTACAA AAATGTGAAC 
ATGTGGAACA AAAACGTCCC AAATGGCAAC AGCCGTTCAG ACCAGCAAGC ACAGGTACAT 
CAGCTGCTAA CGCCTGCAAG TGCCATCTCA AACAAAGAGG CAAGCAGTGG CTCTGGCTGG 
GGTGAGCCCT GGGGGGAGCC TTCTACTCCA GCCACAACTG TGGATAATGG TACTTCAGCA 
TGGGGTAAGC CCATAGACAG TGGTCCCAGC TGGGGGGAAC CCATTGCTGC GGCATCCAGC 
ACATCCACGT GGGGCTCCAG CTCTGTTGGT CCACAAGCAT TAAGCAAATC TGGGCCAAAA 
TCTATGCAAG ATGGCTGGTG TGGTGATGAT ATGCCATTGC CTGGAAATCG CCCCACTGGC 
TGGGAAGAGG AAGAGGATGT GGAGATTGGA ATGTGGAATA GTAATTCATC TCAAGAGCTT 
AACTCATCTT TAAATTGGCC ACCATATACA AAGAAAATGT CATCGAAGGG TCTGAGTGGC 
AAAAAAAGGA GAAGGGAAAG GGGAATGATG AAAGGTGGAA ACAAACAAGA AGAAGCGTGG 
ATAAATCCAT TTGTTAAACA GTTTTCAAAC ATCAGTTTTT CGAGAGACTC ACCAGAGGAA 
AATGTACAAA GCAATAAGAT GGACCTTTCT GGAGGAATGT TACAAGACAA ACGAATGGAG 
ATAGATAAAC ATAGCCTAAA TATTGGTGAT TACAATCGAA CGGTCGGGAA AGGCCCTGGT 
TCTCGGCCTC AGATTTCCAA AGAGTCTTCC ATGGAGCGCA ATCCTTATTT TGATAAGAAT 
GGCAATCCCA GTATGTTTGG TGTTGGAAAC ACAGCAGCAC AACCCCGGGG CATGCAGCAG 
CCTCCAGCAC AACCTCTTAG TTCATCTCAG CCTAATCTCC GTGCTCAAGT GCCTCCTCCA 
TTACTCTCCC CTCAGGTTCC AGTTTCATTG CTGAAGTATG CACCAAACAA CGGTGGCCTG 
AATCCACTCT TTGGCCCTCA ACAGGTAGCC ATGCTGAACC AGCTATCCCA GCTAAACCAG 
CTTTCTCAGA TCTCCCAGTT ACAGCGATTG TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG 
AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG CAAGACCAGC AGGGTCGACC TCTTAGTGTG 
CAGCAGCAAA TGATGCAACA ATCTCGTCAA CTTGATCCAA ACCTGTTGGT GAAGCAGCAG 
ACTCCACCAT CTCAGCAGCA GCCACTCCAT CAGCCAGCCA TGAAGTCTTT CCTTGACAAT 
GTCATGCCCC ACACTACACC TGAGCTGCAA AAAGGGCCAT CACCAATAAA TGCTTTCAGC 
AACTTCCCTA TAGGCTTGAA CTCAAACTTG AATGTAAATA TGGATATGAA CAGTATTAAA 
GAGCCACAGT CAAGACTAAG GAAGTGGACG ACAGTGGACA GCATTTCTGT GAACACATCT 
TTGGATCAAA ACTCCAGCAA ACATGGTGCT ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT 
CCATTTGTTC CCTATGACTT TATGAACAGC AGTACTTCAC CAGCCAGTCC TCCAGGTTCA 
ATAGGAGATG GCTGGCCACG TGCCAAATCG CCTAACGGCT CTAGCAGTGT TAATTGGCCA 
CCAGAATTTC GTCCTGGTGA GCCATGGAAA GGTTATCCAA ACATTGACCC TGAAACTGAC 
CCTTACGTCA CTCCTGGCAG TGTCATAAAC AATCTTTCAA TTAATACTGT GCGGGAAGTT 
GACCACCTCA GGGACAGGAA CAGTGGGTCA TCCTCATCCT TGAACACCAC GCTGCCTTCA 
ACTAGTGCCT GGTCATCCAT TCGTGCCTCC AACTACAACG TTCCCCTCAG CAGTACAGCA 
CAAAGCACTT CAGCCAGAAA TAGTGATTCC AAATTGACAT GGTCTCCTGG TTCAGTTACA 
AACACCTCTC TGGCTCATGA GCTGTGGAAG GTCCCTTTGC CACCTAAAAA CATCACTGCT 
CCGTCCCGCC CACCTCCGGG ACTGACTGGT CAGAAGCCAC CCTTGTCTAC GTGGGATAAT 
TCTCCCCTTC GTATAGGTGG AGGATGGGGA AATTCTGACG CCAGATATAC CCCAGGTTCC 
AGCTGGGGTG AGAGCAGCTC AGGGAGAATA ACAAATTGGC TTGTTCTAAA AAACCTTACA 
CCTCAGATCG ATGGCTCAAC TCTGCGCACT CTGTGCATGC AGCACGGCCC GCTGATCACA 
TTCCACCTGA ACCTCCCTCA CGGAAATGCT CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA 
GTGAAGGCAC AAAAGTCTCT GCACATGTGT GTACTGGGGA ACACTACTAT TCTTGCTGAG 
TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC TTTGCACAAA GCCAGTCTCT GACCCCTTCT 
CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC 
TCATTCTCCA GCCGGACCGA TCTCAATCAC TGGAATGGTG CTGGGCTGTC GGGAACTAAC 
TGTGGAGACC TTCACGGCAC TTCACTCTGG GGGACCCCGC ATTATTCCAC AAGCCTGTGG 
GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA ATTAGCAGCC CATCTCCCAT TAACGCTTTT 
CTTTCTGTTG ACCACCTGGG TGGGGGTGGA GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_001351850.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338779.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.

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CloneID	OHu85977
Clone ID Related Accession (Same CDS sequence)	NM_001351850.1 , NM_001351850.2 , XM_017023147.1
Accession Version	NM_001351850.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010321.8, AC008731.8 and BU743266.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.156185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338779.1
CDS	323..6091
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_001351850.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351850.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( NM_001351850.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338779.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.

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CloneID	OHu85977
Clone ID Related Accession (Same CDS sequence)	NM_001351850.1 , NM_001351850.2 , XM_017023147.1
Accession Version	NM_001351850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010321.8 and AC008731.8. On May 31, 2019 this sequence version replaced NM_001351850.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.156185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338779.1
CDS	327..6095
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	NM_001351850.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351850.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023143.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878632.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

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CloneID	OHu85973
Clone ID Related Accession (Same CDS sequence)	XM_017023143.1 , XM_024450231.1
Accession Version	XM_017023143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878632.1
CDS	32..5947
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023143.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023143.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024450231.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305999.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X1, mRNA.

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CloneID	OHu85973
Clone ID Related Accession (Same CDS sequence)	XM_017023143.1 , XM_024450231.1
Accession Version	XM_024450231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305999.1
CDS	275..6190
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024450231.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450231.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023144.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878633.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OHu85974
Clone ID Related Accession (Same CDS sequence)	XM_017023144.2 , XM_017023144.1
Accession Version	XM_017023144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GGATGGCATT 
GTAGCAGATG AATCCCAAAA CATGCAGTTT ATGTCCAGTC AAAGCATGAA GCTTCCCCCT 
TCAAATAGTG CACTACCTAA CCAGGCCCTT GGCTCCATAG CAGGGCTGGG TATGCAAAAC 
TTGAATTCTG TTAGACAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTTA GTTCATCTCA GCCTAATCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCACTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AGCTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCCGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGTGT GCAGCAGCAA ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAGCA GACTCCACCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGACAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC GACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGTCCTGGTG AGCCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA TTCTCCCCTT CGTATAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGATATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATC GATGGCTCAA CTCTGCGCAC TCTGTGCATG 
CAGCACGGCC CGCTGATCAC ATTCCACCTG AACCTCCCTC ACGGAAATGC TCTGGTCCGC 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGCCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCG 
CATTATTCCA CAAGCCTGTG GGGTCCCCCA AGCAGCAGCG ACCCCCGAGG AATTAGCAGC 
CCATCTCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878633.1
CDS	275..6157
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023144.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023144.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GGATGGCATT 
GTAGCAGATG AATCCCAAAA CATGCAGTTT ATGTCCAGTC AAAGCATGAA GCTTCCCCCT 
TCAAATAGTG CACTACCTAA CCAGGCCCTT GGCTCCATAG CAGGGCTGGG TATGCAAAAC 
TTGAATTCTG TTAGACAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTTA GTTCATCTCA GCCTAATCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCACTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AGCTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCCGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGTGT GCAGCAGCAA ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAGCA GACTCCACCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGACAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC GACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGTCCTGGTG AGCCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA TTCTCCCCTT CGTATAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGATATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATC GATGGCTCAA CTCTGCGCAC TCTGTGCATG 
CAGCACGGCC CGCTGATCAC ATTCCACCTG AACCTCCCTC ACGGAAATGC TCTGGTCCGC 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGCCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCG 
CATTATTCCA CAAGCCTGTG GGGTCCCCCA AGCAGCAGCG ACCCCCGAGG AATTAGCAGC 
CCATCTCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023144.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878633.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

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CloneID	OHu85974
Clone ID Related Accession (Same CDS sequence)	XM_017023144.2 , XM_017023144.1
Accession Version	XM_017023144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GGATGGCATT 
GTAGCAGATG AATCCCAAAA CATGCAGTTT ATGTCCAGTC AAAGCATGAA GCTTCCCCCT 
TCAAATAGTG CACTACCTAA CCAGGCCCTT GGCTCCATAG CAGGGCTGGG TATGCAAAAC 
TTGAATTCTG TTAGACAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTTA GTTCATCTCA GCCTAATCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCACTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AGCTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCCGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGTGT GCAGCAGCAA ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAGCA GACTCCACCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGACAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC GACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGTCCTGGTG AGCCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA TTCTCCCCTT CGTATAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGATATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATC GATGGCTCAA CTCTGCGCAC TCTGTGCATG 
CAGCACGGCC CGCTGATCAC ATTCCACCTG AACCTCCCTC ACGGAAATGC TCTGGTCCGC 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGCCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCG 
CATTATTCCA CAAGCCTGTG GGGTCCCCCA AGCAGCAGCG ACCCCCGAGG AATTAGCAGC 
CCATCTCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878633.1
CDS	32..5914
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023144.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023144.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GGATGGCATT 
GTAGCAGATG AATCCCAAAA CATGCAGTTT ATGTCCAGTC AAAGCATGAA GCTTCCCCCT 
TCAAATAGTG CACTACCTAA CCAGGCCCTT GGCTCCATAG CAGGGCTGGG TATGCAAAAC 
TTGAATTCTG TTAGACAGAA TGGCAATCCC AGTATGTTTG GTGTTGGAAA CACAGCAGCA 
CAACCCCGGG GCATGCAGCA GCCTCCAGCA CAACCTCTTA GTTCATCTCA GCCTAATCTC 
CGTGCTCAAG TGCCTCCTCC ATTACTCTCC CCTCAGGTTC CAGTTTCATT GCTGAAGTAT 
GCACCAAACA ACGGTGGCCT GAATCCACTC TTTGGCCCTC AACAGGTAGC CATGCTGAAC 
CAGCTATCCC AGCTAAACCA GCTTTCTCAG ATCTCCCAGT TACAGCGATT GTTAGCGCAG 
CAGCAAAGGG CGCAGAGTCA GAGAAGCGTG CCTTCTGGGA ACCGGCCGCA GCAAGACCAG 
CAGGGTCGAC CTCTTAGTGT GCAGCAGCAA ATGATGCAAC AATCTCGTCA ACTTGATCCA 
AACCTGTTGG TGAAGCAGCA GACTCCACCA TCTCAGCAGC AGCCACTCCA TCAGCCAGCC 
ATGAAGTCTT TCCTTGACAA TGTCATGCCC CACACTACAC CTGAGCTGCA AAAAGGGCCA 
TCACCAATAA ATGCTTTCAG CAACTTCCCT ATAGGCTTGA ACTCAAACTT GAATGTAAAT 
ATGGATATGA ACAGTATTAA AGAGCCACAG TCAAGACTAA GGAAGTGGAC GACAGTGGAC 
AGCATTTCTG TGAACACATC TTTGGATCAA AACTCCAGCA AACATGGTGC TATTTCAAGT 
GGTTTCAGGC TGGAAGAGTC TCCATTTGTT CCCTATGACT TTATGAACAG CAGTACTTCA 
CCAGCCAGTC CTCCAGGTTC AATAGGAGAT GGCTGGCCAC GTGCCAAATC GCCTAACGGC 
TCTAGCAGTG TTAATTGGCC ACCAGAATTT CGTCCTGGTG AGCCATGGAA AGGTTATCCA 
AACATTGACC CTGAAACTGA CCCTTACGTC ACTCCTGGCA GTGTCATAAA CAATCTTTCA 
ATTAATACTG TGCGGGAAGT TGACCACCTC AGGGACAGGA ACAGTGGGTC ATCCTCATCC 
TTGAACACCA CGCTGCCTTC AACTAGTGCC TGGTCATCCA TTCGTGCCTC CAACTACAAC 
GTTCCCCTCA GCAGTACAGC ACAAAGCACT TCAGCCAGAA ATAGTGATTC CAAATTGACA 
TGGTCTCCTG GTTCAGTTAC AAACACCTCT CTGGCTCATG AGCTGTGGAA GGTCCCTTTG 
CCACCTAAAA ACATCACTGC TCCGTCCCGC CCACCTCCGG GACTGACTGG TCAGAAGCCA 
CCCTTGTCTA CGTGGGATAA TTCTCCCCTT CGTATAGGTG GAGGATGGGG AAATTCTGAC 
GCCAGATATA CCCCAGGTTC CAGCTGGGGT GAGAGCAGCT CAGGGAGAAT AACAAATTGG 
CTTGTTCTAA AAAACCTTAC ACCTCAGATC GATGGCTCAA CTCTGCGCAC TCTGTGCATG 
CAGCACGGCC CGCTGATCAC ATTCCACCTG AACCTCCCTC ACGGAAATGC TCTGGTCCGC 
TACAGTTCAA AAGAAGAGGT AGTGAAGGCA CAAAAGTCTC TGCACATGTG TGTACTGGGG 
AACACTACTA TTCTTGCTGA GTTTGCCAGT GAAGAGGAGA TCAGTCGTTT CTTTGCACAA 
AGCCAGTCTC TGACCCCTTC TCCCGGCTGG CAGTCTCTCG GGTCCAGCCA GAGCCGGCTG 
GGCTCCCTCG ACTGTTCCCA CTCATTCTCC AGCCGGACCG ATCTCAATCA CTGGAATGGT 
GCTGGGCTGT CGGGAACTAA CTGTGGAGAC CTTCACGGCA CTTCACTCTG GGGGACCCCG 
CATTATTCCA CAAGCCTGTG GGGTCCCCCA AGCAGCAGCG ACCCCCGAGG AATTAGCAGC 
CCATCTCCCA TTAACGCTTT TCTTTCTGTT GACCACCTGG GTGGGGGTGG AGAGTCCATG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023145.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878634.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OHu85975
Clone ID Related Accession (Same CDS sequence)	XM_017023145.1 , XM_017023145.2
Accession Version	XM_017023145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TAGCCATGCT GAACCAGCTA TCCCAGCTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC CGCAGCAAGA CCAGCAGGGT CGACCTCTTA GTGTGCAGCA GCAAATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AGCAGACTCC ACCATCTCAG 
CAGCAGCCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG ACAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACGACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGTCCT 
GGTGAGCCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGTTCCC CTCAGCAGTA CAGCACAAAG CACTTCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCT CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TTTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTACGTGGG ATAATTCTCC CCTTCGTATA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGA TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATCGATGGC 
TCAACTCTGC GCACTCTGTG CATGCAGCAC GGCCCGCTGA TCACATTCCA CCTGAACCTC 
CCTCACGGAA ATGCTCTGGT CCGCTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGCCAG TCTCTGACCC CTTCTCCCGG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAC 
GGCACTTCAC TCTGGGGGAC CCCGCATTAT TCCACAAGCC TGTGGGGTCC CCCAAGCAGC 
AGCGACCCCC GAGGAATTAG CAGCCCATCT CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878634.1
CDS	34..5880
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023145.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023145.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TAGCCATGCT GAACCAGCTA TCCCAGCTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC CGCAGCAAGA CCAGCAGGGT CGACCTCTTA GTGTGCAGCA GCAAATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AGCAGACTCC ACCATCTCAG 
CAGCAGCCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG ACAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACGACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGTCCT 
GGTGAGCCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGTTCCC CTCAGCAGTA CAGCACAAAG CACTTCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCT CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TTTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTACGTGGG ATAATTCTCC CCTTCGTATA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGA TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATCGATGGC 
TCAACTCTGC GCACTCTGTG CATGCAGCAC GGCCCGCTGA TCACATTCCA CCTGAACCTC 
CCTCACGGAA ATGCTCTGGT CCGCTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGCCAG TCTCTGACCC CTTCTCCCGG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAC 
GGCACTTCAC TCTGGGGGAC CCCGCATTAT TCCACAAGCC TGTGGGGTCC CCCAAGCAGC 
AGCGACCCCC GAGGAATTAG CAGCCCATCT CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023145.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878634.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X3, mRNA.

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CloneID	OHu85975
Clone ID Related Accession (Same CDS sequence)	XM_017023145.1 , XM_017023145.2
Accession Version	XM_017023145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TAGCCATGCT GAACCAGCTA TCCCAGCTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC CGCAGCAAGA CCAGCAGGGT CGACCTCTTA GTGTGCAGCA GCAAATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AGCAGACTCC ACCATCTCAG 
CAGCAGCCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG ACAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACGACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGTCCT 
GGTGAGCCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGTTCCC CTCAGCAGTA CAGCACAAAG CACTTCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCT CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TTTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTACGTGGG ATAATTCTCC CCTTCGTATA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGA TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATCGATGGC 
TCAACTCTGC GCACTCTGTG CATGCAGCAC GGCCCGCTGA TCACATTCCA CCTGAACCTC 
CCTCACGGAA ATGCTCTGGT CCGCTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGCCAG TCTCTGACCC CTTCTCCCGG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAC 
GGCACTTCAC TCTGGGGGAC CCCGCATTAT TCCACAAGCC TGTGGGGTCC CCCAAGCAGC 
AGCGACCCCC GAGGAATTAG CAGCCCATCT CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878634.1
CDS	275..6121
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023145.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023145.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGGATGGC ATTGTAGCAG ATGAATCCCA AAACATGCAG 
TTTATGTCCA GTCAAAGCAT GAAGCTTCCC CCTTCAAATA GTGCACTACC TAACCAGGCC 
CTTGGCTCCA TAGCAGGGCT GGGTATGCAA AACTTGAATT CTGTTAGACA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TAGCCATGCT GAACCAGCTA TCCCAGCTAA ACCAGCTTTC TCAGATCTCC 
CAGTTACAGC GATTGTTAGC GCAGCAGCAA AGGGCGCAGA GTCAGAGAAG CGTGCCTTCT 
GGGAACCGGC CGCAGCAAGA CCAGCAGGGT CGACCTCTTA GTGTGCAGCA GCAAATGATG 
CAACAATCTC GTCAACTTGA TCCAAACCTG TTGGTGAAGC AGCAGACTCC ACCATCTCAG 
CAGCAGCCAC TCCATCAGCC AGCCATGAAG TCTTTCCTTG ACAATGTCAT GCCCCACACT 
ACACCTGAGC TGCAAAAAGG GCCATCACCA ATAAATGCTT TCAGCAACTT CCCTATAGGC 
TTGAACTCAA ACTTGAATGT AAATATGGAT ATGAACAGTA TTAAAGAGCC ACAGTCAAGA 
CTAAGGAAGT GGACGACAGT GGACAGCATT TCTGTGAACA CATCTTTGGA TCAAAACTCC 
AGCAAACATG GTGCTATTTC AAGTGGTTTC AGGCTGGAAG AGTCTCCATT TGTTCCCTAT 
GACTTTATGA ACAGCAGTAC TTCACCAGCC AGTCCTCCAG GTTCAATAGG AGATGGCTGG 
CCACGTGCCA AATCGCCTAA CGGCTCTAGC AGTGTTAATT GGCCACCAGA ATTTCGTCCT 
GGTGAGCCAT GGAAAGGTTA TCCAAACATT GACCCTGAAA CTGACCCTTA CGTCACTCCT 
GGCAGTGTCA TAAACAATCT TTCAATTAAT ACTGTGCGGG AAGTTGACCA CCTCAGGGAC 
AGGAACAGTG GGTCATCCTC ATCCTTGAAC ACCACGCTGC CTTCAACTAG TGCCTGGTCA 
TCCATTCGTG CCTCCAACTA CAACGTTCCC CTCAGCAGTA CAGCACAAAG CACTTCAGCC 
AGAAATAGTG ATTCCAAATT GACATGGTCT CCTGGTTCAG TTACAAACAC CTCTCTGGCT 
CATGAGCTGT GGAAGGTCCC TTTGCCACCT AAAAACATCA CTGCTCCGTC CCGCCCACCT 
CCGGGACTGA CTGGTCAGAA GCCACCCTTG TCTACGTGGG ATAATTCTCC CCTTCGTATA 
GGTGGAGGAT GGGGAAATTC TGACGCCAGA TATACCCCAG GTTCCAGCTG GGGTGAGAGC 
AGCTCAGGGA GAATAACAAA TTGGCTTGTT CTAAAAAACC TTACACCTCA GATCGATGGC 
TCAACTCTGC GCACTCTGTG CATGCAGCAC GGCCCGCTGA TCACATTCCA CCTGAACCTC 
CCTCACGGAA ATGCTCTGGT CCGCTACAGT TCAAAAGAAG AGGTAGTGAA GGCACAAAAG 
TCTCTGCACA TGTGTGTACT GGGGAACACT ACTATTCTTG CTGAGTTTGC CAGTGAAGAG 
GAGATCAGTC GTTTCTTTGC ACAAAGCCAG TCTCTGACCC CTTCTCCCGG CTGGCAGTCT 
CTCGGGTCCA GCCAGAGCCG GCTGGGCTCC CTCGACTGTT CCCACTCATT CTCCAGCCGG 
ACCGATCTCA ATCACTGGAA TGGTGCTGGG CTGTCGGGAA CTAACTGTGG AGACCTTCAC 
GGCACTTCAC TCTGGGGGAC CCCGCATTAT TCCACAAGCC TGTGGGGTCC CCCAAGCAGC 
AGCGACCCCC GAGGAATTAG CAGCCCATCT CCCATTAACG CTTTTCTTTC TGTTGACCAC 
CTGGGTGGGG GTGGAGAGTC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023146.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878635.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X4, mRNA.

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CloneID	OHu85976
Clone ID Related Accession (Same CDS sequence)	XM_017023146.1
Accession Version	XM_017023146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGATT TAGTGCAAGA AGAAGAACAG TTGATGGAAG AAAAGAAAAA GAAAAAAGAC 
GACAAGAAAA AGAAGGAAGC TGCTCAAAAG AAGGCCACTG AACAAAAAAT CAAAGTGCCA 
GAACAGATAA AGCCCAGTGT AAGCCAGCCT CAGCCTGCCA ACTCTAATAA CGGCACTTCC 
ACAGCAACCA GCACTAATAA TAATGCCAAG CGAGCTACAG CCAACAATCA GCAGCCACAG 
CAGCAGCAGC AACAGCAGCA GCCGCAGCAG CAGCAGCCAC AGCAGCAGCC ACAGCCGCAG 
CCGCAGCAGC AGCAGCCACA GCAGCAGCCA CAGGCCTTGC CTCGGTATCC TCGTGAAGTA 
CCTCCACGAT TTCGCCACCA GGAACACAAA CAGCTTCTAA AGAGGGGTCA GCATTTTCCT 
GTTATAGCAG CAAACCTTGG ATCTGCTGTT AAGGTGTTAA ACAGCCAGTC AGAAAGCAGT 
GCTTTAACAA ATCAACAGCC ACAAAATAAC GGAGAGGTGC AGAACAGCAA AAACCAGTCA 
GATATAAACC ACAGTACTTC AGGATCCCAT TATGAAAATT CCCAGCGGGG ACCTGTGTCT 
TCTACAAGTG ATTCTAGCAC AAACTGTAAG AATGCTGTTG TAAGTGACTT GTCGGAAAAA 
GAAGCATGGC CCTCAGCCCC TGGCAGTGAT CCGGAGTTGG CTTCAGAATG TATGGATGCT 
GATTCTGCCT CCAGTTCTGA ATCAGAGAGA AACATCACTA TCATGGCTTC AGGGAACACA 
GGTGGTGAAA AAGATGGCCT TCGGAATAGC ACTGGACTTG GTTCCCAAAA CAAGTTTGTA 
GTTGGTAGCA GCAGCAATAA TGTGGGCCAT GGAAGTAGTA CTGGGCCATG GGGTTTTTCC 
CATGGAGCCA TAATAAGCAC ATGTCAGGTC TCTGTGGATG CTCCTGAAAG CAAATCTGAA 
AGTAGCAACA ATAGAATGAA TGCTTGGGGC ACTGTAAGTT CTTCATCAAA TGGAGGGTTA 
AATCCAAGCA CTTTGAATTC AGCTAGCAAC CATGGTGCCT GGCCAGTATT AGAGAACAAT 
GGACTTGCCC TAAAAGGGCC TGTAGGGAGT GGTAGTTCTG GCATTAATAT TCAGTGCAGT 
ACTATAGGCC AGATGCCTAA CAATCAGAGT ATTAACTCTA AAGTGAGTGG TGGTTCTACC 
CATGGTACCT GGGGAAGCCT TCAGGAAACT TGTGAATCTG AAGTAAGTGG TACACAGAAG 
GTTTCATTCA GTGGTCAACC TCAAAATATT ACCACTGAAA TGACTGGACC AAATAACACT 
ACTAACTTTA TGACCTCTAG TTTACCAAAC TCCGGTTCAG TGCAGAATAA TGAGCTGCCT 
AGTAGTAACA CAGGGGCCTG GCGTGTGAGC ACAATGAATC ATCCTCAGAT GCAGGCTCCA 
TCAGGTATGA ATGGCACTTC CCTTTCTCAC CTTAGCAATG GAGAGTCAAA AAGTGGAGGC 
TCTTATGGTA CTACATGGGG TGCCTATGGT TCTAATTACT CTGGAGACAA ATGTTCAGGC 
CCTAATGGCC AAGCTAATGG TGACACTGTG AATGCAACTC TAATGCAGCC TGGCGTAAAT 
GGTCCTATGG GCACTAACTT TCAAGTTAAC ACAAACAAAG GAGGTGGTGT GTGGGAATCT 
GGTGCAGCAA ACTCCCAGAG TACATCATGG GGAAGTGGAA ATGGCGCAAA TTCTGGAGGA 
AGTCGAAGAG GATGGGGAAC CCCTGCACAA AACACTGGCA CTAATTTACC CAGCGTTGAG 
TGGAACAAAC TGCCTAGCAA TCAGCATTCC AATGATAGTG CAAATGGCAA TGGTAAGACG 
TTTACAAATG GATGGAAATC TACTGAGGAA GAGGATCAGG GTTCTGCCAC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGCGA TGGTAGTACA 
GAAAGCACTG GACGCCTTGA GGAAAAAGGA ACTGGGGAAA GTCAGAGTAG AGACAGAAGA 
AAAATTGATC AGCACACATT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGTTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CTAGAGGGGA ACGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCATGT ATAGATAAGA CTAGCCCTAA TGGTAATGAT 
ACCTCATCTG TATCAGGGTG GGGCGATCCC AAACCTGCTC TGAGGTGGGG AGATTCCAAA 
GGCTCAAACT GCCAGGGGGG GTGGGAAGAT GATTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAATTGCAA AGAGGAGAAG GCTGCATGGA ATGACTCGCA AAAGAATAAA 
CAGGGATGGG GTGATGGACA AAAATCAAGC CAAGGGTGGT CTGTTTCTGC CAGTGATAAC 
TGGGGAGAAA CTTCAAGGAA TAACCATTGG GGTGAGGCCA ATAAGAAATC CAGCTCAGGA 
GGTAGTGACA GTGACAGGTC CGTTTCCGGT TGGAACGAAC TTGGTAAAAC TAGTTCTTTC 
ACTTGGGGAA ACAACATAAA TCCAAATAAT TCATCAGGAT GGGATGAATC TTCTAAACCT 
ACTCCTTCCC AGGGATGGGG AGACCCTCCA AAGTCTAATC AGTCTCTAGG TTGGGGAGAT 
TCGTCAAAGC CAGTCAGCTC TCCAGACTGG AACAAGCAAC AAGACATTGT TGGATCTTGG 
GGAATCCCAC CAGCTACAGG CAAACCTCCT GGTACAGGCT GGCTGGGGGG ACCTATACCA 
GCCCCAGCAA AAGAAGAAGA ACCCACAGGC TGGGAGGAAC CATCCCCAGA ATCTATACGT 
CGCAAAATGG AGATTGATGA TGGAACTTCA GCTTGGGGAG ATCCAAGCAA ATACAACTAC 
AAAAATGTGA ACATGTGGAA CAAAAACGTC CCAAATGGCA ACAGCCGTTC AGACCAGCAA 
GCACAGGTAC ATCAGCTGCT AACGCCTGCA AGTGCCATCT CAAACAAAGA GGCAAGCAGT 
GGCTCTGGCT GGGGTGAGCC CTGGGGGGAG CCTTCTACTC CAGCCACAAC TGTGGATAAT 
GGTACTTCAG CATGGGGTAA GCCCATAGAC AGTGGTCCCA GCTGGGGGGA ACCCATTGCT 
GCGGCATCCA GCACATCCAC GTGGGGCTCC AGCTCTGTTG GTCCACAAGC ATTAAGCAAA 
TCTGGGCCAA AATCTATGCA AGATGGCTGG TGTGGTGATG ATATGCCATT GCCTGGAAAT 
CGCCCCACTG GCTGGGAAGA GGAAGAGGAT GTGGAGATTG GAATGTGGAA TAGTAATTCA 
TCTCAAGAGC TTAACTCATC TTTAAATTGG CCACCATATA CAAAGAAAAT GTCATCGAAG 
GGTCTGAGTG GCAAAAAAAG GAGAAGGGAA AGGGGAATGA TGAAAGGTGG AAACAAACAA 
GAAGAAGCGT GGATAAATCC ATTTGTTAAA CAGTTTTCAA ACATCAGTTT TTCGAGAGAC 
TCACCAGAGG AAAATGTACA AAGCAATAAG ATGGACCTTT CTGGAGGAAT GTTACAAGAC 
AAACGAATGG AGATAGATAA ACATAGCCTA AATATTGGTG ATTACAATCG AACGGTCGGG 
AAAGGCCCTG GTTCTCGGCC TCAGATTTCC AAAGAGTCTT CCATGGAGCG CAATCCTTAT 
TTTGATAAGG ATGGCATTGT AGCAGATGAA TCCCAAAACA TGCAGTTTAT GTCCAGTCAA 
AGCATGAAGC TTCCCCCTTC AAATAGTGCA CTACCTAACC AGGCCCTTGG CTCCATAGCA 
GGGCTGGGTA TGCAAAACTT GAATTCTGTT AGACAGAATG GCAATCCCAG TATGTTTGGT 
GTTGGAAACA CAGCAGCACA ACCCCGGGGC ATGCAGCAGC CTCCAGCACA ACCTCTTAGT 
TCATCTCAGC CTAATCTCCG TGCTCAAGTG CCTCCTCCAT TACTCTCCCC TCAGGTTCCA 
GTTTCATTGC TGAAGTATGC ACCAAACAAC GGTGGCCTGA ATCCACTCTT TGGCCCTCAA 
CAGGTAGCCA TGCTGAACCA GCTATCCCAG CTAAACCAGC TTTCTCAGAT CTCCCAGTTA 
CAGCGATTGT TAGCGCAGCA GCAAAGGGCG CAGAGTCAGA GAAGCGTGCC TTCTGGGAAC 
CGGCCGCAGC AAGACCAGCA GGGTCGACCT CTTAGTGTGC AGCAGCAAAT GATGCAACAA 
TCTCGTCAAC TTGATCCAAA CCTGTTGGTG AAGCAGCAGA CTCCACCATC TCAGCAGCAG 
CCACTCCATC AGCCAGCCAT GAAGTCTTTC CTTGACAATG TCATGCCCCA CACTACACCT 
GAGCTGCAAA AAGGGCCATC ACCAATAAAT GCTTTCAGCA ACTTCCCTAT AGGCTTGAAC 
TCAAACTTGA ATGTAAATAT GGATATGAAC AGTATTAAAG AGCCACAGTC AAGACTAAGG 
AAGTGGACGA CAGTGGACAG CATTTCTGTG AACACATCTT TGGATCAAAA CTCCAGCAAA 
CATGGTGCTA TTTCAAGTGG TTTCAGGCTG GAAGAGTCTC CATTTGTTCC CTATGACTTT 
ATGAACAGCA GTACTTCACC AGCCAGTCCT CCAGGTTCAA TAGGAGATGG CTGGCCACGT 
GCCAAATCGC CTAACGGCTC TAGCAGTGTT AATTGGCCAC CAGAATTTCG TCCTGGTGAG 
CCATGGAAAG GTTATCCAAA CATTGACCCT GAAACTGACC CTTACGTCAC TCCTGGCAGT 
GTCATAAACA ATCTTTCAAT TAATACTGTG CGGGAAGTTG ACCACCTCAG GGACAGGAAC 
AGTGGGTCAT CCTCATCCTT GAACACCACG CTGCCTTCAA CTAGTGCCTG GTCATCCATT 
CGTGCCTCCA ACTACAACGT TCCCCTCAGC AGTACAGCAC AAAGCACTTC AGCCAGAAAT 
AGTGATTCCA AATTGACATG GTCTCCTGGT TCAGTTACAA ACACCTCTCT GGCTCATGAG 
CTGTGGAAGG TCCCTTTGCC ACCTAAAAAC ATCACTGCTC CGTCCCGCCC ACCTCCGGGA 
CTGACTGGTC AGAAGCCACC CTTGTCTACG TGGGATAATT CTCCCCTTCG TATAGGTGGA 
GGATGGGGAA ATTCTGACGC CAGATATACC CCAGGTTCCA GCTGGGGTGA GAGCAGCTCA 
GGGAGAATAA CAAATTGGCT TGTTCTAAAA AACCTTACAC CTCAGATCGA TGGCTCAACT 
CTGCGCACTC TGTGCATGCA GCACGGCCCG CTGATCACAT TCCACCTGAA CCTCCCTCAC 
GGAAATGCTC TGGTCCGCTA CAGTTCAAAA GAAGAGGTAG TGAAGGCACA AAAGTCTCTG 
CACATGTGTG TACTGGGGAA CACTACTATT CTTGCTGAGT TTGCCAGTGA AGAGGAGATC 
AGTCGTTTCT TTGCACAAAG CCAGTCTCTG ACCCCTTCTC CCGGCTGGCA GTCTCTCGGG 
TCCAGCCAGA GCCGGCTGGG CTCCCTCGAC TGTTCCCACT CATTCTCCAG CCGGACCGAT 
CTCAATCACT GGAATGGTGC TGGGCTGTCG GGAACTAACT GTGGAGACCT TCACGGCACT 
TCACTCTGGG GGACCCCGCA TTATTCCACA AGCCTGTGGG GTCCCCCAAG CAGCAGCGAC 
CCCCGAGGAA TTAGCAGCCC ATCTCCCATT AACGCTTTTC TTTCTGTTGA CCACCTGGGT 
GGGGGTGGAG AGTCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878635.1
CDS	172..6012
Translation	MRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023146.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023146.1

ORF Insert Sequence:

ATGAGGGATT TAGTGCAAGA AGAAGAACAG TTGATGGAAG AAAAGAAAAA GAAAAAAGAC 
GACAAGAAAA AGAAGGAAGC TGCTCAAAAG AAGGCCACTG AACAAAAAAT CAAAGTGCCA 
GAACAGATAA AGCCCAGTGT AAGCCAGCCT CAGCCTGCCA ACTCTAATAA CGGCACTTCC 
ACAGCAACCA GCACTAATAA TAATGCCAAG CGAGCTACAG CCAACAATCA GCAGCCACAG 
CAGCAGCAGC AACAGCAGCA GCCGCAGCAG CAGCAGCCAC AGCAGCAGCC ACAGCCGCAG 
CCGCAGCAGC AGCAGCCACA GCAGCAGCCA CAGGCCTTGC CTCGGTATCC TCGTGAAGTA 
CCTCCACGAT TTCGCCACCA GGAACACAAA CAGCTTCTAA AGAGGGGTCA GCATTTTCCT 
GTTATAGCAG CAAACCTTGG ATCTGCTGTT AAGGTGTTAA ACAGCCAGTC AGAAAGCAGT 
GCTTTAACAA ATCAACAGCC ACAAAATAAC GGAGAGGTGC AGAACAGCAA AAACCAGTCA 
GATATAAACC ACAGTACTTC AGGATCCCAT TATGAAAATT CCCAGCGGGG ACCTGTGTCT 
TCTACAAGTG ATTCTAGCAC AAACTGTAAG AATGCTGTTG TAAGTGACTT GTCGGAAAAA 
GAAGCATGGC CCTCAGCCCC TGGCAGTGAT CCGGAGTTGG CTTCAGAATG TATGGATGCT 
GATTCTGCCT CCAGTTCTGA ATCAGAGAGA AACATCACTA TCATGGCTTC AGGGAACACA 
GGTGGTGAAA AAGATGGCCT TCGGAATAGC ACTGGACTTG GTTCCCAAAA CAAGTTTGTA 
GTTGGTAGCA GCAGCAATAA TGTGGGCCAT GGAAGTAGTA CTGGGCCATG GGGTTTTTCC 
CATGGAGCCA TAATAAGCAC ATGTCAGGTC TCTGTGGATG CTCCTGAAAG CAAATCTGAA 
AGTAGCAACA ATAGAATGAA TGCTTGGGGC ACTGTAAGTT CTTCATCAAA TGGAGGGTTA 
AATCCAAGCA CTTTGAATTC AGCTAGCAAC CATGGTGCCT GGCCAGTATT AGAGAACAAT 
GGACTTGCCC TAAAAGGGCC TGTAGGGAGT GGTAGTTCTG GCATTAATAT TCAGTGCAGT 
ACTATAGGCC AGATGCCTAA CAATCAGAGT ATTAACTCTA AAGTGAGTGG TGGTTCTACC 
CATGGTACCT GGGGAAGCCT TCAGGAAACT TGTGAATCTG AAGTAAGTGG TACACAGAAG 
GTTTCATTCA GTGGTCAACC TCAAAATATT ACCACTGAAA TGACTGGACC AAATAACACT 
ACTAACTTTA TGACCTCTAG TTTACCAAAC TCCGGTTCAG TGCAGAATAA TGAGCTGCCT 
AGTAGTAACA CAGGGGCCTG GCGTGTGAGC ACAATGAATC ATCCTCAGAT GCAGGCTCCA 
TCAGGTATGA ATGGCACTTC CCTTTCTCAC CTTAGCAATG GAGAGTCAAA AAGTGGAGGC 
TCTTATGGTA CTACATGGGG TGCCTATGGT TCTAATTACT CTGGAGACAA ATGTTCAGGC 
CCTAATGGCC AAGCTAATGG TGACACTGTG AATGCAACTC TAATGCAGCC TGGCGTAAAT 
GGTCCTATGG GCACTAACTT TCAAGTTAAC ACAAACAAAG GAGGTGGTGT GTGGGAATCT 
GGTGCAGCAA ACTCCCAGAG TACATCATGG GGAAGTGGAA ATGGCGCAAA TTCTGGAGGA 
AGTCGAAGAG GATGGGGAAC CCCTGCACAA AACACTGGCA CTAATTTACC CAGCGTTGAG 
TGGAACAAAC TGCCTAGCAA TCAGCATTCC AATGATAGTG CAAATGGCAA TGGTAAGACG 
TTTACAAATG GATGGAAATC TACTGAGGAA GAGGATCAGG GTTCTGCCAC ATCTCAGACA 
AATGAGCAAA GCAGTGTGTG GGCCAAAACA GGAGGTACAG TGGAGAGCGA TGGTAGTACA 
GAAAGCACTG GACGCCTTGA GGAAAAAGGA ACTGGGGAAA GTCAGAGTAG AGACAGAAGA 
AAAATTGATC AGCACACATT ACTCCAAAGC ATTGTAAACA GAACTGACTT AGATCCACGT 
GTCCTGTCCA ACTCTGGTTG GGGACAGACT CCTATTAAGC AGAATACTGC CTGGGATACA 
GAAACATCAC CTAGAGGGGA ACGAAAGACT GACAATGGGA CAGAGGCCTG GGGAAGCTCT 
GCAACACAGA CTTTTAACTC AGGGGCATGT ATAGATAAGA CTAGCCCTAA TGGTAATGAT 
ACCTCATCTG TATCAGGGTG GGGCGATCCC AAACCTGCTC TGAGGTGGGG AGATTCCAAA 
GGCTCAAACT GCCAGGGGGG GTGGGAAGAT GATTCTGCTG CTACAGGAAT GGTCAAGAGC 
AATCAGTGGG GGAATTGCAA AGAGGAGAAG GCTGCATGGA ATGACTCGCA AAAGAATAAA 
CAGGGATGGG GTGATGGACA AAAATCAAGC CAAGGGTGGT CTGTTTCTGC CAGTGATAAC 
TGGGGAGAAA CTTCAAGGAA TAACCATTGG GGTGAGGCCA ATAAGAAATC CAGCTCAGGA 
GGTAGTGACA GTGACAGGTC CGTTTCCGGT TGGAACGAAC TTGGTAAAAC TAGTTCTTTC 
ACTTGGGGAA ACAACATAAA TCCAAATAAT TCATCAGGAT GGGATGAATC TTCTAAACCT 
ACTCCTTCCC AGGGATGGGG AGACCCTCCA AAGTCTAATC AGTCTCTAGG TTGGGGAGAT 
TCGTCAAAGC CAGTCAGCTC TCCAGACTGG AACAAGCAAC AAGACATTGT TGGATCTTGG 
GGAATCCCAC CAGCTACAGG CAAACCTCCT GGTACAGGCT GGCTGGGGGG ACCTATACCA 
GCCCCAGCAA AAGAAGAAGA ACCCACAGGC TGGGAGGAAC CATCCCCAGA ATCTATACGT 
CGCAAAATGG AGATTGATGA TGGAACTTCA GCTTGGGGAG ATCCAAGCAA ATACAACTAC 
AAAAATGTGA ACATGTGGAA CAAAAACGTC CCAAATGGCA ACAGCCGTTC AGACCAGCAA 
GCACAGGTAC ATCAGCTGCT AACGCCTGCA AGTGCCATCT CAAACAAAGA GGCAAGCAGT 
GGCTCTGGCT GGGGTGAGCC CTGGGGGGAG CCTTCTACTC CAGCCACAAC TGTGGATAAT 
GGTACTTCAG CATGGGGTAA GCCCATAGAC AGTGGTCCCA GCTGGGGGGA ACCCATTGCT 
GCGGCATCCA GCACATCCAC GTGGGGCTCC AGCTCTGTTG GTCCACAAGC ATTAAGCAAA 
TCTGGGCCAA AATCTATGCA AGATGGCTGG TGTGGTGATG ATATGCCATT GCCTGGAAAT 
CGCCCCACTG GCTGGGAAGA GGAAGAGGAT GTGGAGATTG GAATGTGGAA TAGTAATTCA 
TCTCAAGAGC TTAACTCATC TTTAAATTGG CCACCATATA CAAAGAAAAT GTCATCGAAG 
GGTCTGAGTG GCAAAAAAAG GAGAAGGGAA AGGGGAATGA TGAAAGGTGG AAACAAACAA 
GAAGAAGCGT GGATAAATCC ATTTGTTAAA CAGTTTTCAA ACATCAGTTT TTCGAGAGAC 
TCACCAGAGG AAAATGTACA AAGCAATAAG ATGGACCTTT CTGGAGGAAT GTTACAAGAC 
AAACGAATGG AGATAGATAA ACATAGCCTA AATATTGGTG ATTACAATCG AACGGTCGGG 
AAAGGCCCTG GTTCTCGGCC TCAGATTTCC AAAGAGTCTT CCATGGAGCG CAATCCTTAT 
TTTGATAAGG ATGGCATTGT AGCAGATGAA TCCCAAAACA TGCAGTTTAT GTCCAGTCAA 
AGCATGAAGC TTCCCCCTTC AAATAGTGCA CTACCTAACC AGGCCCTTGG CTCCATAGCA 
GGGCTGGGTA TGCAAAACTT GAATTCTGTT AGACAGAATG GCAATCCCAG TATGTTTGGT 
GTTGGAAACA CAGCAGCACA ACCCCGGGGC ATGCAGCAGC CTCCAGCACA ACCTCTTAGT 
TCATCTCAGC CTAATCTCCG TGCTCAAGTG CCTCCTCCAT TACTCTCCCC TCAGGTTCCA 
GTTTCATTGC TGAAGTATGC ACCAAACAAC GGTGGCCTGA ATCCACTCTT TGGCCCTCAA 
CAGGTAGCCA TGCTGAACCA GCTATCCCAG CTAAACCAGC TTTCTCAGAT CTCCCAGTTA 
CAGCGATTGT TAGCGCAGCA GCAAAGGGCG CAGAGTCAGA GAAGCGTGCC TTCTGGGAAC 
CGGCCGCAGC AAGACCAGCA GGGTCGACCT CTTAGTGTGC AGCAGCAAAT GATGCAACAA 
TCTCGTCAAC TTGATCCAAA CCTGTTGGTG AAGCAGCAGA CTCCACCATC TCAGCAGCAG 
CCACTCCATC AGCCAGCCAT GAAGTCTTTC CTTGACAATG TCATGCCCCA CACTACACCT 
GAGCTGCAAA AAGGGCCATC ACCAATAAAT GCTTTCAGCA ACTTCCCTAT AGGCTTGAAC 
TCAAACTTGA ATGTAAATAT GGATATGAAC AGTATTAAAG AGCCACAGTC AAGACTAAGG 
AAGTGGACGA CAGTGGACAG CATTTCTGTG AACACATCTT TGGATCAAAA CTCCAGCAAA 
CATGGTGCTA TTTCAAGTGG TTTCAGGCTG GAAGAGTCTC CATTTGTTCC CTATGACTTT 
ATGAACAGCA GTACTTCACC AGCCAGTCCT CCAGGTTCAA TAGGAGATGG CTGGCCACGT 
GCCAAATCGC CTAACGGCTC TAGCAGTGTT AATTGGCCAC CAGAATTTCG TCCTGGTGAG 
CCATGGAAAG GTTATCCAAA CATTGACCCT GAAACTGACC CTTACGTCAC TCCTGGCAGT 
GTCATAAACA ATCTTTCAAT TAATACTGTG CGGGAAGTTG ACCACCTCAG GGACAGGAAC 
AGTGGGTCAT CCTCATCCTT GAACACCACG CTGCCTTCAA CTAGTGCCTG GTCATCCATT 
CGTGCCTCCA ACTACAACGT TCCCCTCAGC AGTACAGCAC AAAGCACTTC AGCCAGAAAT 
AGTGATTCCA AATTGACATG GTCTCCTGGT TCAGTTACAA ACACCTCTCT GGCTCATGAG 
CTGTGGAAGG TCCCTTTGCC ACCTAAAAAC ATCACTGCTC CGTCCCGCCC ACCTCCGGGA 
CTGACTGGTC AGAAGCCACC CTTGTCTACG TGGGATAATT CTCCCCTTCG TATAGGTGGA 
GGATGGGGAA ATTCTGACGC CAGATATACC CCAGGTTCCA GCTGGGGTGA GAGCAGCTCA 
GGGAGAATAA CAAATTGGCT TGTTCTAAAA AACCTTACAC CTCAGATCGA TGGCTCAACT 
CTGCGCACTC TGTGCATGCA GCACGGCCCG CTGATCACAT TCCACCTGAA CCTCCCTCAC 
GGAAATGCTC TGGTCCGCTA CAGTTCAAAA GAAGAGGTAG TGAAGGCACA AAAGTCTCTG 
CACATGTGTG TACTGGGGAA CACTACTATT CTTGCTGAGT TTGCCAGTGA AGAGGAGATC 
AGTCGTTTCT TTGCACAAAG CCAGTCTCTG ACCCCTTCTC CCGGCTGGCA GTCTCTCGGG 
TCCAGCCAGA GCCGGCTGGG CTCCCTCGAC TGTTCCCACT CATTCTCCAG CCGGACCGAT 
CTCAATCACT GGAATGGTGC TGGGCTGTCG GGAACTAACT GTGGAGACCT TCACGGCACT 
TCACTCTGGG GGACCCCGCA TTATTCCACA AGCCTGTGGG GTCCCCCAAG CAGCAGCGAC 
CCCCGAGGAA TTAGCAGCCC ATCTCCCATT AACGCTTTTC TTTCTGTTGA CCACCTGGGT 
GGGGGTGGAG AGTCCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023147.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878636.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

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CloneID	OHu85977
Clone ID Related Accession (Same CDS sequence)	NM_001351850.1 , NM_001351850.2 , XM_017023147.1
Accession Version	XM_017023147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878636.1
CDS	272..6040
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023147.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023147.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTTCCAGT TTCATTGCTG 
AAGTATGCAC CAAACAACGG TGGCCTGAAT CCACTCTTTG GCCCTCAACA GGTAGCCATG 
CTGAACCAGC TATCCCAGCT AAACCAGCTT TCTCAGATCT CCCAGTTACA GCGATTGTTA 
GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA AGCGTGCCTT CTGGGAACCG GCCGCAGCAA 
GACCAGCAGG GTCGACCTCT TAGTGTGCAG CAGCAAATGA TGCAACAATC TCGTCAACTT 
GATCCAAACC TGTTGGTGAA GCAGCAGACT CCACCATCTC AGCAGCAGCC ACTCCATCAG 
CCAGCCATGA AGTCTTTCCT TGACAATGTC ATGCCCCACA CTACACCTGA GCTGCAAAAA 
GGGCCATCAC CAATAAATGC TTTCAGCAAC TTCCCTATAG GCTTGAACTC AAACTTGAAT 
GTAAATATGG ATATGAACAG TATTAAAGAG CCACAGTCAA GACTAAGGAA GTGGACGACA 
GTGGACAGCA TTTCTGTGAA CACATCTTTG GATCAAAACT CCAGCAAACA TGGTGCTATT 
TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA TTTGTTCCCT ATGACTTTAT GAACAGCAGT 
ACTTCACCAG CCAGTCCTCC AGGTTCAATA GGAGATGGCT GGCCACGTGC CAAATCGCCT 
AACGGCTCTA GCAGTGTTAA TTGGCCACCA GAATTTCGTC CTGGTGAGCC ATGGAAAGGT 
TATCCAAACA TTGACCCTGA AACTGACCCT TACGTCACTC CTGGCAGTGT CATAAACAAT 
CTTTCAATTA ATACTGTGCG GGAAGTTGAC CACCTCAGGG ACAGGAACAG TGGGTCATCC 
TCATCCTTGA ACACCACGCT GCCTTCAACT AGTGCCTGGT CATCCATTCG TGCCTCCAAC 
TACAACGTTC CCCTCAGCAG TACAGCACAA AGCACTTCAG CCAGAAATAG TGATTCCAAA 
TTGACATGGT CTCCTGGTTC AGTTACAAAC ACCTCTCTGG CTCATGAGCT GTGGAAGGTC 
CCTTTGCCAC CTAAAAACAT CACTGCTCCG TCCCGCCCAC CTCCGGGACT GACTGGTCAG 
AAGCCACCCT TGTCTACGTG GGATAATTCT CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT 
TCTGACGCCA GATATACCCC AGGTTCCAGC TGGGGTGAGA GCAGCTCAGG GAGAATAACA 
AATTGGCTTG TTCTAAAAAA CCTTACACCT CAGATCGATG GCTCAACTCT GCGCACTCTG 
TGCATGCAGC ACGGCCCGCT GATCACATTC CACCTGAACC TCCCTCACGG AAATGCTCTG 
GTCCGCTACA GTTCAAAAGA AGAGGTAGTG AAGGCACAAA AGTCTCTGCA CATGTGTGTA 
CTGGGGAACA CTACTATTCT TGCTGAGTTT GCCAGTGAAG AGGAGATCAG TCGTTTCTTT 
GCACAAAGCC AGTCTCTGAC CCCTTCTCCC GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC 
CGGCTGGGCT CCCTCGACTG TTCCCACTCA TTCTCCAGCC GGACCGATCT CAATCACTGG 
AATGGTGCTG GGCTGTCGGG AACTAACTGT GGAGACCTTC ACGGCACTTC ACTCTGGGGG 
ACCCCGCATT ATTCCACAAG CCTGTGGGGT CCCCCAAGCA GCAGCGACCC CCGAGGAATT 
AGCAGCCCAT CTCCCATTAA CGCTTTTCTT TCTGTTGACC ACCTGGGTGG GGGTGGAGAG 
TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023148.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878637.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OHu85978
Clone ID Related Accession (Same CDS sequence)	XM_017023148.1 , XM_017023148.2
Accession Version	XM_017023148.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTCCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCACTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAGCT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GCGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCCGCAGCAA GACCAGCAGG GTCGACCTCT TAGTGTGCAG 
CAGCAAATGA TGCAACAATC TCGTCAACTT GATCCAAACC TGTTGGTGAA GCAGCAGACT 
CCACCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AGTCTTTCCT TGACAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAAAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCTATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACGACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCACCAG CCAGTCCTCC AGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA TTGGCCACCA 
GAATTTCGTC CTGGTGAGCC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTGCCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT GACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAATTCT 
CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GATATACCCC AGGTTCCAGC 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAAAA CCTTACACCT 
CAGATCGATG GCTCAACTCT GCGCACTCTG TGCATGCAGC ACGGCCCGCT GATCACATTC 
CACCTGAACC TCCCTCACGG AAATGCTCTG GTCCGCTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA CTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG TCGTTTCTTT GCACAAAGCC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCA 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCGCATT ATTCCACAAG CCTGTGGGGT 
CCCCCAAGCA GCAGCGACCC CCGAGGAATT AGCAGCCCAT CTCCCATTAA CGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878637.1
CDS	29..5767
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023148.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023148.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTCCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCACTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAGCT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GCGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCCGCAGCAA GACCAGCAGG GTCGACCTCT TAGTGTGCAG 
CAGCAAATGA TGCAACAATC TCGTCAACTT GATCCAAACC TGTTGGTGAA GCAGCAGACT 
CCACCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AGTCTTTCCT TGACAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAAAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCTATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACGACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCACCAG CCAGTCCTCC AGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA TTGGCCACCA 
GAATTTCGTC CTGGTGAGCC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTGCCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT GACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAATTCT 
CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GATATACCCC AGGTTCCAGC 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAAAA CCTTACACCT 
CAGATCGATG GCTCAACTCT GCGCACTCTG TGCATGCAGC ACGGCCCGCT GATCACATTC 
CACCTGAACC TCCCTCACGG AAATGCTCTG GTCCGCTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA CTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG TCGTTTCTTT GCACAAAGCC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCA 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCGCATT ATTCCACAAG CCTGTGGGGT 
CCCCCAAGCA GCAGCGACCC CCGAGGAATT AGCAGCCCAT CTCCCATTAA CGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023148.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878637.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X5, mRNA.

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CloneID	OHu85978
Clone ID Related Accession (Same CDS sequence)	XM_017023148.1 , XM_017023148.2
Accession Version	XM_017023148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTCCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCACTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAGCT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GCGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCCGCAGCAA GACCAGCAGG GTCGACCTCT TAGTGTGCAG 
CAGCAAATGA TGCAACAATC TCGTCAACTT GATCCAAACC TGTTGGTGAA GCAGCAGACT 
CCACCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AGTCTTTCCT TGACAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAAAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCTATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACGACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCACCAG CCAGTCCTCC AGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA TTGGCCACCA 
GAATTTCGTC CTGGTGAGCC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTGCCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT GACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAATTCT 
CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GATATACCCC AGGTTCCAGC 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAAAA CCTTACACCT 
CAGATCGATG GCTCAACTCT GCGCACTCTG TGCATGCAGC ACGGCCCGCT GATCACATTC 
CACCTGAACC TCCCTCACGG AAATGCTCTG GTCCGCTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA CTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG TCGTTTCTTT GCACAAAGCC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCA 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCGCATT ATTCCACAAG CCTGTGGGGT 
CCCCCAAGCA GCAGCGACCC CCGAGGAATT AGCAGCCCAT CTCCCATTAA CGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878637.1
CDS	275..6013
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKDGIVADESQNMQFMSSQSMKLPPSNSALPNQALGSIAGLGMQNLNSVRQNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023148.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023148.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGGAT GGCATTGTAG CAGATGAATC CCAAAACATG 
CAGTTTATGT CCAGTCAAAG CATGAAGCTT CCCCCTTCAA ATAGTGCACT ACCTAACCAG 
GCCCTTGGCT CCATAGCAGG GCTGGGTATG CAAAACTTGA ATTCTGTTAG ACAGAATGGC 
AATCCCAGTA TGTTTGGTGT TGGAAACACA GCAGCACAAC CCCGGGGCAT GCAGCAGCCT 
CCAGCACAAC CTCTTAGTTC ATCTCAGCCT AATCTCCGTG CTCAAGTGCC TCCTCCATTA 
CTCTCCCCTC AGGTTCCAGT TTCATTGCTG AAGTATGCAC CAAACAACGG TGGCCTGAAT 
CCACTCTTTG GCCCTCAACA GGTAGCCATG CTGAACCAGC TATCCCAGCT AAACCAGCTT 
TCTCAGATCT CCCAGTTACA GCGATTGTTA GCGCAGCAGC AAAGGGCGCA GAGTCAGAGA 
AGCGTGCCTT CTGGGAACCG GCCGCAGCAA GACCAGCAGG GTCGACCTCT TAGTGTGCAG 
CAGCAAATGA TGCAACAATC TCGTCAACTT GATCCAAACC TGTTGGTGAA GCAGCAGACT 
CCACCATCTC AGCAGCAGCC ACTCCATCAG CCAGCCATGA AGTCTTTCCT TGACAATGTC 
ATGCCCCACA CTACACCTGA GCTGCAAAAA GGGCCATCAC CAATAAATGC TTTCAGCAAC 
TTCCCTATAG GCTTGAACTC AAACTTGAAT GTAAATATGG ATATGAACAG TATTAAAGAG 
CCACAGTCAA GACTAAGGAA GTGGACGACA GTGGACAGCA TTTCTGTGAA CACATCTTTG 
GATCAAAACT CCAGCAAACA TGGTGCTATT TCAAGTGGTT TCAGGCTGGA AGAGTCTCCA 
TTTGTTCCCT ATGACTTTAT GAACAGCAGT ACTTCACCAG CCAGTCCTCC AGGTTCAATA 
GGAGATGGCT GGCCACGTGC CAAATCGCCT AACGGCTCTA GCAGTGTTAA TTGGCCACCA 
GAATTTCGTC CTGGTGAGCC ATGGAAAGGT TATCCAAACA TTGACCCTGA AACTGACCCT 
TACGTCACTC CTGGCAGTGT CATAAACAAT CTTTCAATTA ATACTGTGCG GGAAGTTGAC 
CACCTCAGGG ACAGGAACAG TGGGTCATCC TCATCCTTGA ACACCACGCT GCCTTCAACT 
AGTGCCTGGT CATCCATTCG TGCCTCCAAC TACAACGTTC CCCTCAGCAG TACAGCACAA 
AGCACTTCAG CCAGAAATAG TGATTCCAAA TTGACATGGT CTCCTGGTTC AGTTACAAAC 
ACCTCTCTGG CTCATGAGCT GTGGAAGGTC CCTTTGCCAC CTAAAAACAT CACTGCTCCG 
TCCCGCCCAC CTCCGGGACT GACTGGTCAG AAGCCACCCT TGTCTACGTG GGATAATTCT 
CCCCTTCGTA TAGGTGGAGG ATGGGGAAAT TCTGACGCCA GATATACCCC AGGTTCCAGC 
TGGGGTGAGA GCAGCTCAGG GAGAATAACA AATTGGCTTG TTCTAAAAAA CCTTACACCT 
CAGATCGATG GCTCAACTCT GCGCACTCTG TGCATGCAGC ACGGCCCGCT GATCACATTC 
CACCTGAACC TCCCTCACGG AAATGCTCTG GTCCGCTACA GTTCAAAAGA AGAGGTAGTG 
AAGGCACAAA AGTCTCTGCA CATGTGTGTA CTGGGGAACA CTACTATTCT TGCTGAGTTT 
GCCAGTGAAG AGGAGATCAG TCGTTTCTTT GCACAAAGCC AGTCTCTGAC CCCTTCTCCC 
GGCTGGCAGT CTCTCGGGTC CAGCCAGAGC CGGCTGGGCT CCCTCGACTG TTCCCACTCA 
TTCTCCAGCC GGACCGATCT CAATCACTGG AATGGTGCTG GGCTGTCGGG AACTAACTGT 
GGAGACCTTC ACGGCACTTC ACTCTGGGGG ACCCCGCATT ATTCCACAAG CCTGTGGGGT 
CCCCCAAGCA GCAGCGACCC CCGAGGAATT AGCAGCCCAT CTCCCATTAA CGCTTTTCTT 
TCTGTTGACC ACCTGGGTGG GGGTGGAGAG TCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024450232.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306000.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X6, mRNA.

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CloneID	OHu85979
Clone ID Related Accession (Same CDS sequence)	XM_024450232.1 , XM_017023149.1
Accession Version	XM_024450232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306000.1
CDS	275..6010
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024450232.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450232.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023149.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878638.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

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CloneID	OHu85979
Clone ID Related Accession (Same CDS sequence)	XM_024450232.1 , XM_017023149.1
Accession Version	XM_017023149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878638.1
CDS	29..5764
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023149.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023149.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGAAT GATGAAAGGT GGAAACAAAC AAGAAGAAGC GTGGATAAAT 
CCATTTGTTA AACAGTTTTC AAACATCAGT TTTTCGAGAG ACTCACCAGA GGAAAATGTA 
CAAAGCAATA AGATGGACCT TTCTGGAGGA ATGTTACAAG ACAAACGAAT GGAGATAGAT 
AAACATAGCC TAAATATTGG TGATTACAAT CGAACGGTCG GGAAAGGCCC TGGTTCTCGG 
CCTCAGATTT CCAAAGAGTC TTCCATGGAG CGCAATCCTT ATTTTGATAA GAATGGCAAT 
CCCAGTATGT TTGGTGTTGG AAACACAGCA GCACAACCCC GGGGCATGCA GCAGCCTCCA 
GCACAACCTC TTAGTTCATC TCAGCCTAAT CTCCGTGCTC AAGTGCCTCC TCCATTACTC 
TCCCCTCAGG TTCCAGTTTC ATTGCTGAAG TATGCACCAA ACAACGGTGG CCTGAATCCA 
CTCTTTGGCC CTCAACAGGT AGCCATGCTG AACCAGCTAT CCCAGCTAAA CCAGCTTTCT 
CAGATCTCCC AGTTACAGCG ATTGTTAGCG CAGCAGCAAA GGGCGCAGAG TCAGAGAAGC 
GTGCCTTCTG GGAACCGGCC GCAGCAAGAC CAGCAGGGTC GACCTCTTAG TGTGCAGCAG 
CAAATGATGC AACAATCTCG TCAACTTGAT CCAAACCTGT TGGTGAAGCA GCAGACTCCA 
CCATCTCAGC AGCAGCCACT CCATCAGCCA GCCATGAAGT CTTTCCTTGA CAATGTCATG 
CCCCACACTA CACCTGAGCT GCAAAAAGGG CCATCACCAA TAAATGCTTT CAGCAACTTC 
CCTATAGGCT TGAACTCAAA CTTGAATGTA AATATGGATA TGAACAGTAT TAAAGAGCCA 
CAGTCAAGAC TAAGGAAGTG GACGACAGTG GACAGCATTT CTGTGAACAC ATCTTTGGAT 
CAAAACTCCA GCAAACATGG TGCTATTTCA AGTGGTTTCA GGCTGGAAGA GTCTCCATTT 
GTTCCCTATG ACTTTATGAA CAGCAGTACT TCACCAGCCA GTCCTCCAGG TTCAATAGGA 
GATGGCTGGC CACGTGCCAA ATCGCCTAAC GGCTCTAGCA GTGTTAATTG GCCACCAGAA 
TTTCGTCCTG GTGAGCCATG GAAAGGTTAT CCAAACATTG ACCCTGAAAC TGACCCTTAC 
GTCACTCCTG GCAGTGTCAT AAACAATCTT TCAATTAATA CTGTGCGGGA AGTTGACCAC 
CTCAGGGACA GGAACAGTGG GTCATCCTCA TCCTTGAACA CCACGCTGCC TTCAACTAGT 
GCCTGGTCAT CCATTCGTGC CTCCAACTAC AACGTTCCCC TCAGCAGTAC AGCACAAAGC 
ACTTCAGCCA GAAATAGTGA TTCCAAATTG ACATGGTCTC CTGGTTCAGT TACAAACACC 
TCTCTGGCTC ATGAGCTGTG GAAGGTCCCT TTGCCACCTA AAAACATCAC TGCTCCGTCC 
CGCCCACCTC CGGGACTGAC TGGTCAGAAG CCACCCTTGT CTACGTGGGA TAATTCTCCC 
CTTCGTATAG GTGGAGGATG GGGAAATTCT GACGCCAGAT ATACCCCAGG TTCCAGCTGG 
GGTGAGAGCA GCTCAGGGAG AATAACAAAT TGGCTTGTTC TAAAAAACCT TACACCTCAG 
ATCGATGGCT CAACTCTGCG CACTCTGTGC ATGCAGCACG GCCCGCTGAT CACATTCCAC 
CTGAACCTCC CTCACGGAAA TGCTCTGGTC CGCTACAGTT CAAAAGAAGA GGTAGTGAAG 
GCACAAAAGT CTCTGCACAT GTGTGTACTG GGGAACACTA CTATTCTTGC TGAGTTTGCC 
AGTGAAGAGG AGATCAGTCG TTTCTTTGCA CAAAGCCAGT CTCTGACCCC TTCTCCCGGC 
TGGCAGTCTC TCGGGTCCAG CCAGAGCCGG CTGGGCTCCC TCGACTGTTC CCACTCATTC 
TCCAGCCGGA CCGATCTCAA TCACTGGAAT GGTGCTGGGC TGTCGGGAAC TAACTGTGGA 
GACCTTCACG GCACTTCACT CTGGGGGACC CCGCATTATT CCACAAGCCT GTGGGGTCCC 
CCAAGCAGCA GCGACCCCCG AGGAATTAGC AGCCCATCTC CCATTAACGC TTTTCTTTCT 
GTTGACCACC TGGGTGGGGG TGGAGAGTCC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023150.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878639.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

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CloneID	OHu85980
Clone ID Related Accession (Same CDS sequence)	XM_017023150.1 , XM_017023150.2
Accession Version	XM_017023150.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTAGCCAT GCTGAACCAG 
CTATCCCAGC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCCGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGTGTGCA GCAGCAAATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAGCAGAC TCCACCATCT CAGCAGCAGC CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGACAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACGAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGT CCTGGTGAGC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGTT 
CCCCTCAGCA GTACAGCACA AAGCACTTCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCTCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTTTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTACGT GGGATAATTC TCCCCTTCGT ATAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGATATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATCGAT GGCTCAACTC TGCGCACTCT GTGCATGCAG 
CACGGCCCGC TGATCACATT CCACCTGAAC CTCCCTCACG GAAATGCTCT GGTCCGCTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGC 
CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CACGGCACTT CACTCTGGGG GACCCCGCAT 
TATTCCACAA GCCTGTGGGG TCCCCCAAGC AGCAGCGACC CCCGAGGAAT TAGCAGCCCA 
TCTCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878639.1
CDS	30..5729
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023150.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023150.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTAGCCAT GCTGAACCAG 
CTATCCCAGC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCCGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGTGTGCA GCAGCAAATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAGCAGAC TCCACCATCT CAGCAGCAGC CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGACAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACGAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGT CCTGGTGAGC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGTT 
CCCCTCAGCA GTACAGCACA AAGCACTTCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCTCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTTTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTACGT GGGATAATTC TCCCCTTCGT ATAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGATATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATCGAT GGCTCAACTC TGCGCACTCT GTGCATGCAG 
CACGGCCCGC TGATCACATT CCACCTGAAC CTCCCTCACG GAAATGCTCT GGTCCGCTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGC 
CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CACGGCACTT CACTCTGGGG GACCCCGCAT 
TATTCCACAA GCCTGTGGGG TCCCCCAAGC AGCAGCGACC CCCGAGGAAT TAGCAGCCCA 
TCTCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023150.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878639.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X7, mRNA.

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CloneID	OHu85980
Clone ID Related Accession (Same CDS sequence)	XM_017023150.1 , XM_017023150.2
Accession Version	XM_017023150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTAGCCAT GCTGAACCAG 
CTATCCCAGC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCCGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGTGTGCA GCAGCAAATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAGCAGAC TCCACCATCT CAGCAGCAGC CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGACAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACGAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGT CCTGGTGAGC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGTT 
CCCCTCAGCA GTACAGCACA AAGCACTTCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCTCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTTTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTACGT GGGATAATTC TCCCCTTCGT ATAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGATATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATCGAT GGCTCAACTC TGCGCACTCT GTGCATGCAG 
CACGGCCCGC TGATCACATT CCACCTGAAC CTCCCTCACG GAAATGCTCT GGTCCGCTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGC 
CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CACGGCACTT CACTCTGGGG GACCCCGCAT 
TATTCCACAA GCCTGTGGGG TCCCCCAAGC AGCAGCGACC CCCGAGGAAT TAGCAGCCCA 
TCTCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878639.1
CDS	275..5974
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGWGEPWGEPSTPATTVDNGTSAWGKPIDSGPSWGEPIAAASSTSTWGSSSVGPQALSKSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023150.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023150.2

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGCTGGGGT GAGCCCTGGG GGGAGCCTTC TACTCCAGCC 
ACAACTGTGG ATAATGGTAC TTCAGCATGG GGTAAGCCCA TAGACAGTGG TCCCAGCTGG 
GGGGAACCCA TTGCTGCGGC ATCCAGCACA TCCACGTGGG GCTCCAGCTC TGTTGGTCCA 
CAAGCATTAA GCAAATCTGG GCCAAAATCT ATGCAAGATG GCTGGTGTGG TGATGATATG 
CCATTGCCTG GAAATCGCCC CACTGGCTGG GAAGAGGAAG AGGATGTGGA GATTGGAATG 
TGGAATAGTA ATTCATCTCA AGAGCTTAAC TCATCTTTAA ATTGGCCACC ATATACAAAG 
AAAATGTCAT CGAAGGGTCT GAGTGGCAAA AAAAGGAGAA GGGAAAGGGG AATGATGAAA 
GGTGGAAACA AACAAGAAGA AGCGTGGATA AATCCATTTG TTAAACAGTT TTCAAACATC 
AGTTTTTCGA GAGACTCACC AGAGGAAAAT GTACAAAGCA ATAAGATGGA CCTTTCTGGA 
GGAATGTTAC AAGACAAACG AATGGAGATA GATAAACATA GCCTAAATAT TGGTGATTAC 
AATCGAACGG TCGGGAAAGG CCCTGGTTCT CGGCCTCAGA TTTCCAAAGA GTCTTCCATG 
GAGCGCAATC CTTATTTTGA TAAGAATGGC AATCCCAGTA TGTTTGGTGT TGGAAACACA 
GCAGCACAAC CCCGGGGCAT GCAGCAGCCT CCAGCACAAC CTCTTAGTTC ATCTCAGCCT 
AATCTCCGTG CTCAAGTGCC TCCTCCATTA CTCTCCCCTC AGGTAGCCAT GCTGAACCAG 
CTATCCCAGC TAAACCAGCT TTCTCAGATC TCCCAGTTAC AGCGATTGTT AGCGCAGCAG 
CAAAGGGCGC AGAGTCAGAG AAGCGTGCCT TCTGGGAACC GGCCGCAGCA AGACCAGCAG 
GGTCGACCTC TTAGTGTGCA GCAGCAAATG ATGCAACAAT CTCGTCAACT TGATCCAAAC 
CTGTTGGTGA AGCAGCAGAC TCCACCATCT CAGCAGCAGC CACTCCATCA GCCAGCCATG 
AAGTCTTTCC TTGACAATGT CATGCCCCAC ACTACACCTG AGCTGCAAAA AGGGCCATCA 
CCAATAAATG CTTTCAGCAA CTTCCCTATA GGCTTGAACT CAAACTTGAA TGTAAATATG 
GATATGAACA GTATTAAAGA GCCACAGTCA AGACTAAGGA AGTGGACGAC AGTGGACAGC 
ATTTCTGTGA ACACATCTTT GGATCAAAAC TCCAGCAAAC ATGGTGCTAT TTCAAGTGGT 
TTCAGGCTGG AAGAGTCTCC ATTTGTTCCC TATGACTTTA TGAACAGCAG TACTTCACCA 
GCCAGTCCTC CAGGTTCAAT AGGAGATGGC TGGCCACGTG CCAAATCGCC TAACGGCTCT 
AGCAGTGTTA ATTGGCCACC AGAATTTCGT CCTGGTGAGC CATGGAAAGG TTATCCAAAC 
ATTGACCCTG AAACTGACCC TTACGTCACT CCTGGCAGTG TCATAAACAA TCTTTCAATT 
AATACTGTGC GGGAAGTTGA CCACCTCAGG GACAGGAACA GTGGGTCATC CTCATCCTTG 
AACACCACGC TGCCTTCAAC TAGTGCCTGG TCATCCATTC GTGCCTCCAA CTACAACGTT 
CCCCTCAGCA GTACAGCACA AAGCACTTCA GCCAGAAATA GTGATTCCAA ATTGACATGG 
TCTCCTGGTT CAGTTACAAA CACCTCTCTG GCTCATGAGC TGTGGAAGGT CCCTTTGCCA 
CCTAAAAACA TCACTGCTCC GTCCCGCCCA CCTCCGGGAC TGACTGGTCA GAAGCCACCC 
TTGTCTACGT GGGATAATTC TCCCCTTCGT ATAGGTGGAG GATGGGGAAA TTCTGACGCC 
AGATATACCC CAGGTTCCAG CTGGGGTGAG AGCAGCTCAG GGAGAATAAC AAATTGGCTT 
GTTCTAAAAA ACCTTACACC TCAGATCGAT GGCTCAACTC TGCGCACTCT GTGCATGCAG 
CACGGCCCGC TGATCACATT CCACCTGAAC CTCCCTCACG GAAATGCTCT GGTCCGCTAC 
AGTTCAAAAG AAGAGGTAGT GAAGGCACAA AAGTCTCTGC ACATGTGTGT ACTGGGGAAC 
ACTACTATTC TTGCTGAGTT TGCCAGTGAA GAGGAGATCA GTCGTTTCTT TGCACAAAGC 
CAGTCTCTGA CCCCTTCTCC CGGCTGGCAG TCTCTCGGGT CCAGCCAGAG CCGGCTGGGC 
TCCCTCGACT GTTCCCACTC ATTCTCCAGC CGGACCGATC TCAATCACTG GAATGGTGCT 
GGGCTGTCGG GAACTAACTG TGGAGACCTT CACGGCACTT CACTCTGGGG GACCCCGCAT 
TATTCCACAA GCCTGTGGGG TCCCCCAAGC AGCAGCGACC CCCGAGGAAT TAGCAGCCCA 
TCTCCCATTA ACGCTTTTCT TTCTGTTGAC CACCTGGGTG GGGGTGGAGA GTCCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_017023151.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878640.1 Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

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CloneID	OHu85981
Clone ID Related Accession (Same CDS sequence)	XM_017023151.1 , XM_024450233.1
Accession Version	XM_017023151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCACTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA GCTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTG CAGCAGCAAA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAGCAG ACTCCACCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGACAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACG 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GTCCTGGTGA GCCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAT TCTCCCCTTC GTATAGGTGG AGGATGGGGA 
AATTCTGACG CCAGATATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATCG ATGGCTCAAC TCTGCGCACT 
CTGTGCATGC AGCACGGCCC GCTGATCACA TTCCACCTGA ACCTCCCTCA CGGAAATGCT 
CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GCCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCGC ATTATTCCAC AAGCCTGTGG GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA 
ATTAGCAGCC CATCTCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878640.1
CDS	27..5618
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_017023151.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing 6A (TNRC6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023151.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCACTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA GCTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTG CAGCAGCAAA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAGCAG ACTCCACCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGACAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACG 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GTCCTGGTGA GCCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAT TCTCCCCTTC GTATAGGTGG AGGATGGGGA 
AATTCTGACG CCAGATATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATCG ATGGCTCAAC TCTGCGCACT 
CTGTGCATGC AGCACGGCCC GCTGATCACA TTCCACCTGA ACCTCCCTCA CGGAAATGCT 
CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GCCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCGC ATTATTCCAC AAGCCTGTGG GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA 
ATTAGCAGCC CATCTCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNRC6A ( XM_024450233.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306001.1 Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X8, mRNA.

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CloneID	OHu85981
Clone ID Related Accession (Same CDS sequence)	XM_017023151.1 , XM_024450233.1
Accession Version	XM_024450233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	trinucleotide repeat-containing gene 6A protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCACTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA GCTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTG CAGCAGCAAA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAGCAG ACTCCACCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGACAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACG 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GTCCTGGTGA GCCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAT TCTCCCCTTC GTATAGGTGG AGGATGGGGA 
AATTCTGACG CCAGATATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATCG ATGGCTCAAC TCTGCGCACT 
CTGTGCATGC AGCACGGCCC GCTGATCACA TTCCACCTGA ACCTCCCTCA CGGAAATGCT 
CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GCCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCGC ATTATTCCAC AAGCCTGTGG GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA 
ATTAGCAGCC CATCTCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306001.1
CDS	275..5866
Translation	MQLVAEPGLDARCPGSQDIPLPEEWNRDLVQEEEQLMEEKKKKKDDKKKKEAAQKKATEQKIKVPEQIKPSVSQPQPANSNNGTSTATSTNNNAKRATANNQQPQQQQQQQQPQQQQPQQQPQPQPQQQQPQQQPQALPRYPREVPPRFRHQEHKQLLKRGQHFPVIAANLGSAVKVLNSQSESSALTNQQPQNNGEVQNSKNQSDINHSTSGSHYENSQRGPVSSTSDSSTNCKNAVVSDLSEKEAWPSAPGSDPELASECMDADSASSSESERNITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWGTVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAANSQSTSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSRDRRKIDQHTLLQSIVNRTDLDPRVLSNSGWGQTPIKQNTAWDTETSPRGERKTDNGTEAWGSSATQTFNSGACIDKTSPNGNDTSSVSGWGDPKPALRWGDSKGSNCQGGWEDDSAATGMVKSNQWGNCKEEKAAWNDSQKNKQGWGDGQKSSQGWSVSASDNWGETSRNNHWGEANKKSSSGGSDSDRSVSGWNELGKTSSFTWGNNINPNNSSGWDESSKPTPSQGWGDPPKSNQSLGWGDSSKPVSSPDWNKQQDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAISNKEASSGSGPKSMQDGWCGDDMPLPGNRPTGWEEEEDVEIGMWNSNSSQELNSSLNWPPYTKKMSSKGLSGKKRRRERGMMKGGNKQEEAWINPFVKQFSNISFSRDSPEENVQSNKMDLSGGMLQDKRMEIDKHSLNIGDYNRTVGKGPGSRPQISKESSMERNPYFDKNGNPSMFGVGNTAAQPRGMQQPPAQPLSSSQPNLRAQVPPPLLSPQVPVSLLKYAPNNGGLNPLFGPQQVAMLNQLSQLNQLSQISQLQRLLAQQQRAQSQRSVPSGNRPQQDQQGRPLSVQQQMMQQSRQLDPNLLVKQQTPPSQQQPLHQPAMKSFLDNVMPHTTPELQKGPSPINAFSNFPIGLNSNLNVNMDMNSIKEPQSRLRKWTTVDSISVNTSLDQNSSKHGAISSGFRLEESPFVPYDFMNSSTSPASPPGSIGDGWPRAKSPNGSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDHLRDRNSGSSSSLNTTLPSTSAWSSIRASNYNVPLSSTAQSTSARNSDSKLTWSPGSVTNTSLAHELWKVPLPPKNITAPSRPPPGLTGQKPPLSTWDNSPLRIGGGWGNSDARYTPGSSWGESSSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFAQSQSLTPSPGWQSLGSSQSRLGSLDCSHSFSSRTDLNHWNGAGLSGTNCGDLHGTSLWGTPHYSTSLWGPPSSSDPRGISSPSPINAFLSVDHLGGGGESM

Target ORF information:

RefSeq Version	XM_024450233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trinucleotide repeat containing adaptor 6A (TNRC6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450233.1

ORF Insert Sequence:

ATGCAACTAG TGGCAGAGCC AGGCCTAGAC GCCAGGTGTC CTGGCTCTCA AGACATTCCG 
CTGCCCGAGG AATGGAACAG GGATTTAGTG CAAGAAGAAG AACAGTTGAT GGAAGAAAAG 
AAAAAGAAAA AAGACGACAA GAAAAAGAAG GAAGCTGCTC AAAAGAAGGC CACTGAACAA 
AAAATCAAAG TGCCAGAACA GATAAAGCCC AGTGTAAGCC AGCCTCAGCC TGCCAACTCT 
AATAACGGCA CTTCCACAGC AACCAGCACT AATAATAATG CCAAGCGAGC TACAGCCAAC 
AATCAGCAGC CACAGCAGCA GCAGCAACAG CAGCAGCCGC AGCAGCAGCA GCCACAGCAG 
CAGCCACAGC CGCAGCCGCA GCAGCAGCAG CCACAGCAGC AGCCACAGGC CTTGCCTCGG 
TATCCTCGTG AAGTACCTCC ACGATTTCGC CACCAGGAAC ACAAACAGCT TCTAAAGAGG 
GGTCAGCATT TTCCTGTTAT AGCAGCAAAC CTTGGATCTG CTGTTAAGGT GTTAAACAGC 
CAGTCAGAAA GCAGTGCTTT AACAAATCAA CAGCCACAAA ATAACGGAGA GGTGCAGAAC 
AGCAAAAACC AGTCAGATAT AAACCACAGT ACTTCAGGAT CCCATTATGA AAATTCCCAG 
CGGGGACCTG TGTCTTCTAC AAGTGATTCT AGCACAAACT GTAAGAATGC TGTTGTAAGT 
GACTTGTCGG AAAAAGAAGC ATGGCCCTCA GCCCCTGGCA GTGATCCGGA GTTGGCTTCA 
GAATGTATGG ATGCTGATTC TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG 
GCTTCAGGGA ACACAGGTGG TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC 
CAAAACAAGT TTGTAGTTGG TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG 
CCATGGGGTT TTTCCCATGG AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT 
GAAAGCAAAT CTGAAAGTAG CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA 
TCAAATGGAG GGTTAAATCC AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA 
GTATTAGAGA ACAATGGACT TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT 
AATATTCAGT GCAGTACTAT AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG 
AGTGGTGGTT CTACCCATGG TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA 
AGTGGTACAC AGAAGGTTTC ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT 
GGACCAAATA ACACTACTAA CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG 
AATAATGAGC TGCCTAGTAG TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT 
CAGATGCAGG CTCCATCAGG TATGAATGGC ACTTCCCTTT CTCACCTTAG CAATGGAGAG 
TCAAAAAGTG GAGGCTCTTA TGGTACTACA TGGGGTGCCT ATGGTTCTAA TTACTCTGGA 
GACAAATGTT CAGGCCCTAA TGGCCAAGCT AATGGTGACA CTGTGAATGC AACTCTAATG 
CAGCCTGGCG TAAATGGTCC TATGGGCACT AACTTTCAAG TTAACACAAA CAAAGGAGGT 
GGTGTGTGGG AATCTGGTGC AGCAAACTCC CAGAGTACAT CATGGGGAAG TGGAAATGGC 
GCAAATTCTG GAGGAAGTCG AAGAGGATGG GGAACCCCTG CACAAAACAC TGGCACTAAT 
TTACCCAGCG TTGAGTGGAA CAAACTGCCT AGCAATCAGC ATTCCAATGA TAGTGCAAAT 
GGCAATGGTA AGACGTTTAC AAATGGATGG AAATCTACTG AGGAAGAGGA TCAGGGTTCT 
GCCACATCTC AGACAAATGA GCAAAGCAGT GTGTGGGCCA AAACAGGAGG TACAGTGGAG 
AGCGATGGTA GTACAGAAAG CACTGGACGC CTTGAGGAAA AAGGAACTGG GGAAAGTCAG 
AGTAGAGACA GAAGAAAAAT TGATCAGCAC ACATTACTCC AAAGCATTGT AAACAGAACT 
GACTTAGATC CACGTGTCCT GTCCAACTCT GGTTGGGGAC AGACTCCTAT TAAGCAGAAT 
ACTGCCTGGG ATACAGAAAC ATCACCTAGA GGGGAACGAA AGACTGACAA TGGGACAGAG 
GCCTGGGGAA GCTCTGCAAC ACAGACTTTT AACTCAGGGG CATGTATAGA TAAGACTAGC 
CCTAATGGTA ATGATACCTC ATCTGTATCA GGGTGGGGCG ATCCCAAACC TGCTCTGAGG 
TGGGGAGATT CCAAAGGCTC AAACTGCCAG GGGGGGTGGG AAGATGATTC TGCTGCTACA 
GGAATGGTCA AGAGCAATCA GTGGGGGAAT TGCAAAGAGG AGAAGGCTGC ATGGAATGAC 
TCGCAAAAGA ATAAACAGGG ATGGGGTGAT GGACAAAAAT CAAGCCAAGG GTGGTCTGTT 
TCTGCCAGTG ATAACTGGGG AGAAACTTCA AGGAATAACC ATTGGGGTGA GGCCAATAAG 
AAATCCAGCT CAGGAGGTAG TGACAGTGAC AGGTCCGTTT CCGGTTGGAA CGAACTTGGT 
AAAACTAGTT CTTTCACTTG GGGAAACAAC ATAAATCCAA ATAATTCATC AGGATGGGAT 
GAATCTTCTA AACCTACTCC TTCCCAGGGA TGGGGAGACC CTCCAAAGTC TAATCAGTCT 
CTAGGTTGGG GAGATTCGTC AAAGCCAGTC AGCTCTCCAG ACTGGAACAA GCAACAAGAC 
ATTGTTGGAT CTTGGGGAAT CCCACCAGCT ACAGGCAAAC CTCCTGGTAC AGGCTGGCTG 
GGGGGACCTA TACCAGCCCC AGCAAAAGAA GAAGAACCCA CAGGCTGGGA GGAACCATCC 
CCAGAATCTA TACGTCGCAA AATGGAGATT GATGATGGAA CTTCAGCTTG GGGAGATCCA 
AGCAAATACA ACTACAAAAA TGTGAACATG TGGAACAAAA ACGTCCCAAA TGGCAACAGC 
CGTTCAGACC AGCAAGCACA GGTACATCAG CTGCTAACGC CTGCAAGTGC CATCTCAAAC 
AAAGAGGCAA GCAGTGGCTC TGGGCCAAAA TCTATGCAAG ATGGCTGGTG TGGTGATGAT 
ATGCCATTGC CTGGAAATCG CCCCACTGGC TGGGAAGAGG AAGAGGATGT GGAGATTGGA 
ATGTGGAATA GTAATTCATC TCAAGAGCTT AACTCATCTT TAAATTGGCC ACCATATACA 
AAGAAAATGT CATCGAAGGG TCTGAGTGGC AAAAAAAGGA GAAGGGAAAG GGGAATGATG 
AAAGGTGGAA ACAAACAAGA AGAAGCGTGG ATAAATCCAT TTGTTAAACA GTTTTCAAAC 
ATCAGTTTTT CGAGAGACTC ACCAGAGGAA AATGTACAAA GCAATAAGAT GGACCTTTCT 
GGAGGAATGT TACAAGACAA ACGAATGGAG ATAGATAAAC ATAGCCTAAA TATTGGTGAT 
TACAATCGAA CGGTCGGGAA AGGCCCTGGT TCTCGGCCTC AGATTTCCAA AGAGTCTTCC 
ATGGAGCGCA ATCCTTATTT TGATAAGAAT GGCAATCCCA GTATGTTTGG TGTTGGAAAC 
ACAGCAGCAC AACCCCGGGG CATGCAGCAG CCTCCAGCAC AACCTCTTAG TTCATCTCAG 
CCTAATCTCC GTGCTCAAGT GCCTCCTCCA TTACTCTCCC CTCAGGTTCC AGTTTCATTG 
CTGAAGTATG CACCAAACAA CGGTGGCCTG AATCCACTCT TTGGCCCTCA ACAGGTAGCC 
ATGCTGAACC AGCTATCCCA GCTAAACCAG CTTTCTCAGA TCTCCCAGTT ACAGCGATTG 
TTAGCGCAGC AGCAAAGGGC GCAGAGTCAG AGAAGCGTGC CTTCTGGGAA CCGGCCGCAG 
CAAGACCAGC AGGGTCGACC TCTTAGTGTG CAGCAGCAAA TGATGCAACA ATCTCGTCAA 
CTTGATCCAA ACCTGTTGGT GAAGCAGCAG ACTCCACCAT CTCAGCAGCA GCCACTCCAT 
CAGCCAGCCA TGAAGTCTTT CCTTGACAAT GTCATGCCCC ACACTACACC TGAGCTGCAA 
AAAGGGCCAT CACCAATAAA TGCTTTCAGC AACTTCCCTA TAGGCTTGAA CTCAAACTTG 
AATGTAAATA TGGATATGAA CAGTATTAAA GAGCCACAGT CAAGACTAAG GAAGTGGACG 
ACAGTGGACA GCATTTCTGT GAACACATCT TTGGATCAAA ACTCCAGCAA ACATGGTGCT 
ATTTCAAGTG GTTTCAGGCT GGAAGAGTCT CCATTTGTTC CCTATGACTT TATGAACAGC 
AGTACTTCAC CAGCCAGTCC TCCAGGTTCA ATAGGAGATG GCTGGCCACG TGCCAAATCG 
CCTAACGGCT CTAGCAGTGT TAATTGGCCA CCAGAATTTC GTCCTGGTGA GCCATGGAAA 
GGTTATCCAA ACATTGACCC TGAAACTGAC CCTTACGTCA CTCCTGGCAG TGTCATAAAC 
AATCTTTCAA TTAATACTGT GCGGGAAGTT GACCACCTCA GGGACAGGAA CAGTGGGTCA 
TCCTCATCCT TGAACACCAC GCTGCCTTCA ACTAGTGCCT GGTCATCCAT TCGTGCCTCC 
AACTACAACG TTCCCCTCAG CAGTACAGCA CAAAGCACTT CAGCCAGAAA TAGTGATTCC 
AAATTGACAT GGTCTCCTGG TTCAGTTACA AACACCTCTC TGGCTCATGA GCTGTGGAAG 
GTCCCTTTGC CACCTAAAAA CATCACTGCT CCGTCCCGCC CACCTCCGGG ACTGACTGGT 
CAGAAGCCAC CCTTGTCTAC GTGGGATAAT TCTCCCCTTC GTATAGGTGG AGGATGGGGA 
AATTCTGACG CCAGATATAC CCCAGGTTCC AGCTGGGGTG AGAGCAGCTC AGGGAGAATA 
ACAAATTGGC TTGTTCTAAA AAACCTTACA CCTCAGATCG ATGGCTCAAC TCTGCGCACT 
CTGTGCATGC AGCACGGCCC GCTGATCACA TTCCACCTGA ACCTCCCTCA CGGAAATGCT 
CTGGTCCGCT ACAGTTCAAA AGAAGAGGTA GTGAAGGCAC AAAAGTCTCT GCACATGTGT 
GTACTGGGGA ACACTACTAT TCTTGCTGAG TTTGCCAGTG AAGAGGAGAT CAGTCGTTTC 
TTTGCACAAA GCCAGTCTCT GACCCCTTCT CCCGGCTGGC AGTCTCTCGG GTCCAGCCAG 
AGCCGGCTGG GCTCCCTCGA CTGTTCCCAC TCATTCTCCA GCCGGACCGA TCTCAATCAC 
TGGAATGGTG CTGGGCTGTC GGGAACTAAC TGTGGAGACC TTCACGGCAC TTCACTCTGG 
GGGACCCCGC ATTATTCCAC AAGCCTGTGG GGTCCCCCAA GCAGCAGCGA CCCCCGAGGA 
ATTAGCAGCC CATCTCCCAT TAACGCTTTT CTTTCTGTTG ACCACCTGGG TGGGGGTGGA 
GAGTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry.
Journal of the American Society for Mass Spectrometry(2017 Oct)
Cieplak-Rotowska MK,Tarnowski K,Rubin M,Fabian MR,Sonenberg N,Dadlez M,Niedzwiecka A

Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.
The Journal of cell biology216(10)3073-3085(2017 Oct)
Kourtidis A,Necela B,Lin WH,Lu R,Feathers RW,Asmann YW,Thompson EA,Anastasiadis PZ

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オンライン注文

TNRC6A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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