NIPBL cDNA ORF clone, Bos taurus(cattle)

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa94122 XM_015459095.2
Latest version!
Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa239220 NM_001206584.2
Latest version!
Bos taurus NIPBL cohesin loading factor (NIPBL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa40744 NM_001206584.1
Latest version!
Bos taurus NIPBL, cohesin loading factor (NIPBL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228057 XM_024981354.1
Latest version!
Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa228058 XM_005221614.3
Latest version!
Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa30682 XM_005221614.2
Latest version!
Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OBa94122 XM_015459095.1 Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa94122
    Clone ID Related Accession (Same CDS sequence) XM_015459095.2 , XM_015459095.1
    Accession Version XM_015459095.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nipped-B-like protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT
    CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314581.1
    CDS500..7288
    Translation

    Target ORF information:

    RefSeq Version XM_015459095.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459095.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT
    CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94122
    Clone ID Related Accession (Same CDS sequence) XM_015459095.2 , XM_015459095.1
    Accession Version XM_015459095.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT
    CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314581.1
    CDS423..7211
    Translation

    Target ORF information:

    RefSeq Version XM_015459095.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459095.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT
    ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC
    ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA
    CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC
    TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA
    AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT
    GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT
    CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA
    GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC
    ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT
    GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC
    AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT
    GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG
    TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG
    AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC
    ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT
    CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA
    GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA
    ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA
    ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT
    TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC
    TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA
    TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA
    GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA
    CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT
    CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT
    TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT
    GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA
    CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG
    ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA
    GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA
    GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC
    ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT
    GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA
    GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC
    CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA
    ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT
    ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT
    GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA
    GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT
    AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA
    CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC
    AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT
    CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT
    AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC
    AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT
    ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC
    TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT
    CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG
    GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA
    CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239220
    Clone ID Related Accession (Same CDS sequence) NM_001206584.2
    Accession Version NM_001206584.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-29
    Organism Bos taurus(cattle)
    Product nipped-B-like protein
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000020.1. On Dec 20, 2018 this sequence version replaced NM_001206584.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGUY01036956.1, GGVC01060376.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193513.1
    CDS502..8916
    Translation

    Target ORF information:

    RefSeq Version NM_001206584.2
    Organism Bos taurus(cattle)
    Definition Bos taurus NIPBL cohesin loading factor (NIPBL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206584.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40744
    Clone ID Related Accession (Same CDS sequence) NM_001206584.1
    Accession Version NM_001206584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product nipped-B-like protein
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02050625.1, DAAA02050622.1, DAAA02050620.1 and DAAA02050619.1. On or before May 4, 2011 this sequence version replaced XM_002696375.1, XM_588088.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193513.1
    CDS502..8916
    Translation

    Target ORF information:

    RefSeq Version NM_001206584.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NIPBL, cohesin loading factor (NIPBL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG
    ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC
    CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG
    GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG
    CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT
    GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT
    GGGACTTCCA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228057
    Clone ID Related Accession (Same CDS sequence) XM_024981354.1
    Accession Version XM_024981354.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837122.1
    CDS500..8668
    Translation

    Target ORF information:

    RefSeq Version XM_024981354.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATACACAAAA TGATTTCATG
    GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT
    GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG
    ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT
    TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT
    CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA
    TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA
    GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG
    CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG
    CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT
    AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA
    GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA
    GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC
    CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC
    AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT
    TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT
    GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG
    ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC
    CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC
    CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA
    ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA
    CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT
    AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA
    TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG
    TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG
    ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT
    ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT
    AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG
    AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT
    AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA
    CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC
    ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT
    GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA
    GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT
    GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA
    CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC
    TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG
    GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT
    AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT
    TCCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228058
    Clone ID Related Accession (Same CDS sequence) XM_005221614.3
    Accession Version XM_005221614.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nipped-B-like protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221671.1
    CDS500..8596
    Translation

    Target ORF information:

    RefSeq Version XM_005221614.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221614.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30682
    Clone ID Related Accession (Same CDS sequence) XM_005221614.2
    Accession Version XM_005221614.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product nipped-B-like protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221671.1
    CDS423..8519
    Translation

    Target ORF information:

    RefSeq Version XM_005221614.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221614.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
    GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT
    CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT
    TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA
    GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG
    GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA
    ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC
    AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA
    AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA
    CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG
    ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT
    AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA
    CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT
    TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC
    CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG
    TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA
    AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG
    AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA
    AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG
    GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT
    TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG
    TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC
    ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA
    CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA
    GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG
    CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT
    AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG
    GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT
    TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT
    CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT
    AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG
    GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA
    GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA
    TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC
    ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC
    GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG
    CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT
    GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT
    AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT
    AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC
    TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT
    AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT
    CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT
    TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA
    CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT
    AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG
    ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA
    TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA
    ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA
    GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG
    CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA
    GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA
    GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA
    TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT
    GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT
    GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT
    GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT
    TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT
    CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG
    CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG
    AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA
    TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA
    CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC
    AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC
    ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT
    ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC
    CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT
    AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT
    CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG
    AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA
    CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA
    CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA
    GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG
    GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG
    GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT
    GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA
    GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC
    TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA
    CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT
    GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA
    ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG
    AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG
    GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT
    GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG
    TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA
    TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT
    GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA
    TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG
    GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC
    CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT
    GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA
    ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT
    TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT
    GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG
    ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG
    GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC
    AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC
    AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG
    GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG
    CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA
    AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG
    GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT
    TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT
    CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT
    GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA
    CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA
    AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT
    TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT
    TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT
    ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT
    ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA
    TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC
    CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA
    CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG
    CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC
    GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG
    CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG
    CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC
    ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG
    GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT
    GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA
    GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC
    CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT
    GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA
    AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT
    CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT
    TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC
    CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT
    GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA
    AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT
    ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT
    TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA
    GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA
    AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC
    ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.