NIPBL gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005248282.4	XP_005248339.3	nipped-B-like protein isoform X5
XM_006714467.2	XP_006714530.1	nipped-B-like protein isoform X1
XM_006714468.2	XP_006714531.1	nipped-B-like protein isoform X2
NM_133433.3	NP_597677.2	nipped-B-like protein isoform A
XM_005248282.5	XP_005248339.3	nipped-B-like protein isoform X5
NM_015384.5	NP_056199.2	nipped-B-like protein isoform B
XM_017009329.1	XP_016864818.1	nipped-B-like protein isoform X4
XM_011514015.1	XP_011512317.1	nipped-B-like protein isoform X6
NM_015384.4	NP_056199.2	nipped-B-like protein isoform B
XM_017009330.1	XP_016864819.1	nipped-B-like protein isoform X7
NM_133433.4	NP_597677.2	nipped-B-like protein isoform A
XM_017009331.1	XP_016864820.1	nipped-B-like protein isoform X8
XM_005248280.3	XP_005248337.1	nipped-B-like protein isoform X3
XM_017009330.2	XP_016864819.1	nipped-B-like protein isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26171	NM_133433.4 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu95815	XM_017009331.1 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu26399	NM_015384.5 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu39306	XM_006714467.2 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu39307	XM_006714468.2 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu39308	XM_005248280.3 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu66530	XM_011514015.1 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu95812	XM_017009329.1 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu95813	XM_005248282.5 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu95814	XM_017009330.2 Latest version!	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
View Old Accession Versions >>
OHu95813	XM_005248282.4	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu26399	NM_015384.4	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu26171	NM_133433.3	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu95814	XM_017009330.1	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

NIPBL ( NM_133433.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_597677.2 Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu26171
Clone ID Related Accession (Same CDS sequence)	NM_133433.3 , NM_133433.4
Accession Version	NM_133433.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced NM_133433.2. Transcript Variant: This variant (A) encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ627032.1, BK005151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597677.2
CDS	500..8914
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	NM_133433.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133433.3

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( NM_133433.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_597677.2 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant A, mRNA.

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CloneID	OHu26171
Clone ID Related Accession (Same CDS sequence)	NM_133433.3 , NM_133433.4
Accession Version	NM_133433.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1, AJ627032.1 and AC008902.5. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_133433.3. Transcript Variant: This variant (A) encodes the longer isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ627032.1, BK005151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000282516.13/ ENSP00000282516.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_597677.2
CDS	490..8904
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	NM_133433.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133433.4

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_017009331.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864820.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X8, mRNA.

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CloneID	OHu95815
Clone ID Related Accession (Same CDS sequence)	XM_017009331.1
Accession Version	XM_017009331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAGTCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAC AAACGCAAGC GTAGCACAGT TAATGAAAAG 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAGC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGTCCGTCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTTGC TAGGAAAATG AAGAAAAAAG AAAAACAGAA GAAAAGGAAA 
GCATATGAAC CAAAACTAAC ACCTGAAGAA ATGATGGACT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAT TTGGAAGATA TGGATTTTAC TGCGTTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATAAA AGCAATGGGT ATAATGGATA AGCTTTCAAC TGACAAAACT 
GTGAAAGTCT TAAATATCTT GGAGAAGAAT ATTCAGGATG GGTCAAAGCT TTCCACTTTG 
TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTTACAA AATCAGCGGA TGCTTGTCTT ACAACTATCA ACATTATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTGTACAT TGAGGATGTA ATTGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAT TAAGTTCAAA AGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAACAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ATTGCTAGAG 
ATACAACTTC TTACAGACAC AACAATTCTT CAGGTTTCAT CTATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAGTTG TGTGCCATTA AGTTAGTCAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA 
TTACCAACCA GCAAGAGGAG TTTAAGGAAC TTCAGGTTAA ACAGTAGTGA TATGGATGGA 
GAACCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTTATTCA GTGTGTGGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT GCAGAAGAAG ATTCAAATAA AAAAATTGAC 
CAGGATGTTG TCATTACTAA CTCTTATGAA ACAGCTATGC GAACAGCCCA AAACTTCCTC 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTGTTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAACAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGAACTG TTGCTGCACG GCTAAGAAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGA TCTATAGAAC GCATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATCCA ACAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACTGAGACTG ATCCTTCACT AGTGTTTTCT CGTAAATTCT ATATAGCCCA GTGGTTTCGA 
GACACAACTC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGAACACATC ATGCAAAGGA AATTGAGACA ACTGGCCAAA TTATGCATCG AGCTGAAAAC 
CGAAAAAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTTAG CACATTAAAG 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGCTTGA TTGTTCGATA CTTGGCCTCC 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTACG AGTTCTTGGT 
GAAAATGCAA TTGCTGTTCG AACAAAAGCC ATGAAGTGTT TGTCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTTGATATG CAACGAGGTG TTCATGGACG ATTGATGGAT 
AATTCGACTA GTGTCCGAGA AGCAGCAGTA GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAACAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGGA AAAGAGTAAT AAAGATTCTC AGAGACATTT GTATTGAACA ACCAACATTT 
CCAAAAATCA CAGAAATGTG TGTAAAAATG ATTCGCAGAG TCAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAGAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA AATTTTAAAC ATTACCGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAACTGCTT CAAAACTTGT TGAAGTCCGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCAATGA CTATGCAACC ATACCTTACC ACTAAATGTA GTACGCAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATCCTAGAG CTAGTTGTAC CACTGATGGA GCATCCAAGT 
GAAACTTTTC TTGCCACTAT TGAGGAAGAT CTAATGAAGC TCATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT GAGCTGTCTT GGAGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCTTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTTCTAACAA ACAAACCAGC ACTTCTTAGA 
TCCCTTTTCA CCGTTGGAGC ACTATGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGCA AGGTTAACAT AAAAGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAA 
CACTCAGATG AAGAAGTACA AACAAAAGCT ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CATCCAAGTC TAATGTTCGA GCAAGAAGTG AAGAATCTAT ATAATAATAT TTTATCTGAT 
AAGAACTCCT CAGTCAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAAGTTGC AAAACAGGAA 
GACTTAAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAT 
CTCAAACAGG TGCTTGAGGC ATTTTTTCAC ACCCAGTCAA GTGTACGCCA CTTTGCCCTA 
AATGTCATTG CATTGACTCT AAATCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCATAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCT GCTATGCGGA ACAAGGCTGA TCAGCAACTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCATATGA AAGCAGTGGC TGGTATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC 
AGACAAGACG AGTCCTCTAG CGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAACACA GACGAGCCTT TCTTATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGACGTGA CTATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCAGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAGGACA AAAGGAAAGA GAGAAAATCA 
TCACCTAGTA AGGAAAATGA GTCAAGCGAC AGTGAAGAAG AAGTTTCCAG GCCTCGGAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCAGATTCAG AAGACGATAT AAATTCAGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATCGAAT TTGCAAATGT GTCCCAGGGT 
ATTTTATTAC TTCTCATGTT AAAACAACAT TTGAAGAATC TTTGTGGATT TTCTGATAGT 
AAAATTCAGA AGTACTCTCC ATCTGAATCT GCAAAAGTAT ATGATAAAGC GATAAACCGA 
AAAACAGGAG TTCATTTTCA TCCAAAACAA ACACTGGACT TCCTGCGGAG TGACATGGCT 
AATTCCAAAA TCACAGAAGA GGTGAAAAGG AGTATAGTAA AACAGTATCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAAGAAGAAG AAGGGGAGGT TTCAGCTAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCA CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
ACAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGATCGACCA 
CAAATTGCAA GAGTAGTGCA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864820.1
CDS	489..7277
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_017009331.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009331.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAGTCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAC AAACGCAAGC GTAGCACAGT TAATGAAAAG 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAGC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG GAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGTCCGTCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTTGC TAGGAAAATG AAGAAAAAAG AAAAACAGAA GAAAAGGAAA 
GCATATGAAC CAAAACTAAC ACCTGAAGAA ATGATGGACT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAT TTGGAAGATA TGGATTTTAC TGCGTTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATAAA AGCAATGGGT ATAATGGATA AGCTTTCAAC TGACAAAACT 
GTGAAAGTCT TAAATATCTT GGAGAAGAAT ATTCAGGATG GGTCAAAGCT TTCCACTTTG 
TTAAATCATA ATAACGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTTACAA AATCAGCGGA TGCTTGTCTT ACAACTATCA ACATTATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTGTACAT TGAGGATGTA ATTGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAT TAAGTTCAAA AGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAACAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ATTGCTAGAG 
ATACAACTTC TTACAGACAC AACAATTCTT CAGGTTTCAT CTATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAGTTG TGTGCCATTA AGTTAGTCAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTTGCAAGA 
TTACCAACCA GCAAGAGGAG TTTAAGGAAC TTCAGGTTAA ACAGTAGTGA TATGGATGGA 
GAACCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTTATTCA GTGTGTGGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT GCAGAAGAAG ATTCAAATAA AAAAATTGAC 
CAGGATGTTG TCATTACTAA CTCTTATGAA ACAGCTATGC GAACAGCCCA AAACTTCCTC 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTGTTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAACAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC TCTTGATTAC CTTGGAACTG TTGCTGCACG GCTAAGAAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGA TCTATAGAAC GCATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATCCA ACAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACTGAGACTG ATCCTTCACT AGTGTTTTCT CGTAAATTCT ATATAGCCCA GTGGTTTCGA 
GACACAACTC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGAACACATC ATGCAAAGGA AATTGAGACA ACTGGCCAAA TTATGCATCG AGCTGAAAAC 
CGAAAAAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTTAG CACATTAAAG 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGCTTGA TTGTTCGATA CTTGGCCTCC 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTACG AGTTCTTGGT 
GAAAATGCAA TTGCTGTTCG AACAAAAGCC ATGAAGTGTT TGTCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTTGATATG CAACGAGGTG TTCATGGACG ATTGATGGAT 
AATTCGACTA GTGTCCGAGA AGCAGCAGTA GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAACAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGGA AAAGAGTAAT AAAGATTCTC AGAGACATTT GTATTGAACA ACCAACATTT 
CCAAAAATCA CAGAAATGTG TGTAAAAATG ATTCGCAGAG TCAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAGAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA AATTTTAAAC ATTACCGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAACTGCTT CAAAACTTGT TGAAGTCCGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCAATGA CTATGCAACC ATACCTTACC ACTAAATGTA GTACGCAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATCCTAGAG CTAGTTGTAC CACTGATGGA GCATCCAAGT 
GAAACTTTTC TTGCCACTAT TGAGGAAGAT CTAATGAAGC TCATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT GAGCTGTCTT GGAGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCTTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTTCTAACAA ACAAACCAGC ACTTCTTAGA 
TCCCTTTTCA CCGTTGGAGC ACTATGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGCA AGGTTAACAT AAAAGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAA 
CACTCAGATG AAGAAGTACA AACAAAAGCT ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CATCCAAGTC TAATGTTCGA GCAAGAAGTG AAGAATCTAT ATAATAATAT TTTATCTGAT 
AAGAACTCCT CAGTCAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAAGTTGC AAAACAGGAA 
GACTTAAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAT 
CTCAAACAGG TGCTTGAGGC ATTTTTTCAC ACCCAGTCAA GTGTACGCCA CTTTGCCCTA 
AATGTCATTG CATTGACTCT AAATCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCATAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCT GCTATGCGGA ACAAGGCTGA TCAGCAACTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCATATGA AAGCAGTGGC TGGTATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACATGCCTAA AAGATCCTGT AAGGGGTTTC 
AGACAAGACG AGTCCTCTAG CGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAACACA GACGAGCCTT TCTTATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGACGTGA CTATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCAGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCTATG GTAAAGGACA AAAGGAAAGA GAGAAAATCA 
TCACCTAGTA AGGAAAATGA GTCAAGCGAC AGTGAAGAAG AAGTTTCCAG GCCTCGGAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCAGATTCAG AAGACGATAT AAATTCAGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATCGAAT TTGCAAATGT GTCCCAGGGT 
ATTTTATTAC TTCTCATGTT AAAACAACAT TTGAAGAATC TTTGTGGATT TTCTGATAGT 
AAAATTCAGA AGTACTCTCC ATCTGAATCT GCAAAAGTAT ATGATAAAGC GATAAACCGA 
AAAACAGGAG TTCATTTTCA TCCAAAACAA ACACTGGACT TCCTGCGGAG TGACATGGCT 
AATTCCAAAA TCACAGAAGA GGTGAAAAGG AGTATAGTAA AACAGTATCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAAGAAGAAG AAGGGGAGGT TTCAGCTAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCA CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
ACAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGATCGACCA 
CAAATTGCAA GAGTAGTGCA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( NM_015384.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056199.2 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.

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CloneID	OHu26399
Clone ID Related Accession (Same CDS sequence)	NM_015384.5 , NM_015384.4
Accession Version	NM_015384.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1, AJ640137.1, AA508213.1, BQ723324.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_015384.4. Transcript Variant: This variant (B) is alternatively spliced at the 3' end compared to transcript variant A. It encodes an isoform (B) with a shorter, distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ640137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056199.2
CDS	490..8583
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT

Target ORF information:

RefSeq Version	NM_015384.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015384.5

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( NM_015384.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056199.2 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.

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CloneID	OHu26399
Clone ID Related Accession (Same CDS sequence)	NM_015384.5 , NM_015384.4
Accession Version	NM_015384.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN369580.1, AJ640137.1, AA508213.1, BQ723324.1 and AJ627032.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2008 this sequence version replaced NM_015384.3. Transcript Variant: This variant (B) is alternatively spliced at the 3' end compared to transcript variant A. It encodes an isoform (B) with a shorter, distinct C-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ640137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056199.2
CDS	500..8593
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT

Target ORF information:

RefSeq Version	NM_015384.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015384.4

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_006714467.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714530.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X1, mRNA.

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CloneID	OHu39306
Clone ID Related Accession (Same CDS sequence)	XM_006714467.2
Accession Version	XM_006714467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006714467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG 
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA 
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT 
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA 
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA 
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT 
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG 
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC 
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA 
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA 
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT 
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG 
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT 
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA 
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT 
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714530.1
CDS	489..8756
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTASMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_006714467.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714467.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG 
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA 
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT 
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA 
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA 
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT 
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG 
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC 
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA 
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA 
CGAAATTCAG ACTCTACGGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA CGTCATGGAT 
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTGCAG 
AAAACCAGCA GTGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT 
GAGGCTAAGC GCCGTGATGG CCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA 
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT 
CAGACTTTAC GATCCCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_006714468.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714531.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X2, mRNA.

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CloneID	OHu39307
Clone ID Related Accession (Same CDS sequence)	XM_006714468.2
Accession Version	XM_006714468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA 
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA 
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA 
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT 
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA 
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT 
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA 
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT 
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT 
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA 
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT 
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT 
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT 
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA 
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC 
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT 
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA 
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC 
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT 
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT 
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT 
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA 
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA 
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT 
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT 
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG 
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT 
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT 
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT 
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT 
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT 
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT 
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT 
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT 
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA 
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA 
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA 
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG 
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC 
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT 
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG 
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA 
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT 
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT 
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT 
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA 
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG 
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG 
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC 
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG 
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA 
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA 
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA 
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA 
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA 
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG 
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA 
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG 
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC 
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG 
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG 
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA 
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT 
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG 
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG 
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG 
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT 
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG 
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT 
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG 
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT 
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC 
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA 
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG 
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG 
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA 
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC 
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA 
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC 
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT 
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT 
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714531.1
CDS	489..8705
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFEFARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_006714468.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714468.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATTTGCTA GGAAAATGAA GAAAAAAGAA AAACAGAAGA AAAGGAAAGC ATATGAACCA 
AAACTAACAC CTGAAGAAAT GATGGACTCT TCAACTTTTA AGAGATTCAC AGCCTCAATA 
GAGAATATTT TGGATAATTT GGAAGATATG GATTTTACTG CGTTTGGTGA TGATGATGAA 
ATTCCTCAGG AACTGCTCTT AGGAAAACAT CAGCTTAATG AACTTGGCAG TGAATCTGCT 
AAAATAAAAG CAATGGGTAT AATGGATAAG CTTTCAACTG ACAAAACTGT GAAAGTCTTA 
AATATCTTGG AGAAGAATAT TCAGGATGGG TCAAAGCTTT CCACTTTGTT AAATCATAAT 
AACGATACTG AAGAAGAAGA AAGGTTATGG AGAGACCTTA TTATGGAGAG AGTTACAAAA 
TCAGCGGATG CTTGTCTTAC AACTATCAAC ATTATGACAT CCCCTAACAT GCCAAAAGCT 
GTGTACATTG AGGATGTAAT TGAAAGAGTT ATACAGTACA CTAAATTTCA TTTGCAGAAT 
ACACTTTATC CTCAGTATGA TCCTGTTTAC AGATTAGATC CTCATGGAGG AGGCTTATTA 
AGTTCAAAAG CAAAACGGGC TAAATGTTCT ACCCATAAGC AGAGAGTAAT AGTAATGCTT 
TATAACAAAG TTTGTGACAT TGTTAGCAGC TTATCAGAAT TGCTAGAGAT ACAACTTCTT 
ACAGACACAA CAATTCTTCA GGTTTCATCT ATGGGAATAA CACCATTTTT TGTGGAAAAT 
GTCAGTGAAC TACAGTTGTG TGCCATTAAG TTAGTCACTG CAGTATTCTC AAGATATGAA 
AAACATAGGC AGTTAATTTT GGAAGAAATT TTTACTTCAC TTGCAAGATT ACCAACCAGC 
AAGAGGAGTT TAAGGAACTT CAGGTTAAAC AGTAGTGATA TGGATGGAGA ACCTATGTAT 
ATTCAGATGG TTACAGCACT GGTTTTACAA CTTATTCAGT GTGTGGTACA CTTACCATCA 
TCAGAGAAGG ACTCTAATGC AGAAGAAGAT TCAAATAAAA AAATTGACCA GGATGTTGTC 
ATTACTAACT CTTATGAAAC AGCTATGCGA ACAGCCCAAA ACTTCCTCTC CATCTTCCTT 
AAAAAATGTG GTAGTAAGCA AGGTGAAGAA GATTACAGAC CACTGTTTGA AAATTTTGTT 
CAAGACCTTC TTTCAACAGT CAATAAGCCT GAATGGCCAG CTGCTGAACT ACTCCTTAGT 
TTGTTAGGGA GACTGTTGGT TCATCAGTTC AGTAACAAGT CAACAGAGAT GGCTTTAAGA 
GTGGCATCTC TTGATTACCT TGGAACTGTT GCTGCACGGC TAAGAAAAGA TGCTGTTACA 
AGCAAAATGG ATCAAGGATC TATAGAACGC ATTTTAAAAC AGGTTTCAGG AGGGGAAGAT 
GAAATCCAAC AATTACAAAA AGCATTGCTT GATTACTTGG ATGAAAACAC TGAGACTGAT 
CCTTCACTAG TGTTTTCTCG TAAATTCTAT ATAGCCCAGT GGTTTCGAGA CACAACTCTG 
GAAACAGAAA AAGCAATGAA ATCACAAAAA GATGAAGAAT CATCTGAAGG AACACATCAT 
GCAAAGGAAA TTGAGACAAC TGGCCAAATT ATGCATCGAG CTGAAAACCG AAAAAAGTTT 
CTTAGAAGCA TTATCAAAAC CACACCTTCT CAGTTTAGCA CATTAAAGAT GAACTCTGAT 
ACTGTGGACT ATGATGATGC TTGCTTGATT GTTCGATACT TGGCCTCCAT GAGGCCGTTT 
GCCCAGAGCT TTGATATTTA TTTGACACAG ATCCTACGAG TTCTTGGTGA AAATGCAATT 
GCTGTTCGAA CAAAAGCCAT GAAGTGTTTG TCTGAGGTTG TTGCTGTAGA CCCCAGTATT 
CTAGCAAGGC TTGATATGCA ACGAGGTGTT CATGGACGAT TGATGGATAA TTCGACTAGT 
GTCCGAGAAG CAGCAGTAGA ATTACTAGGT CGATTTGTCC TTTGTCGACC TCAGCTTGCT 
GAACAGTATT ATGATATGCT GATTGAAAGA ATATTGGATA CTGGTATCAG TGTCAGGAAA 
AGAGTAATAA AGATTCTCAG AGACATTTGT ATTGAACAAC CAACATTTCC AAAAATCACA 
GAAATGTGTG TAAAAATGAT TCGCAGAGTC AATGATGAAG AGGGCATTAA GAAATTAGTA 
AATGAAACAT TCCAGAAACT CTGGTTTACT CCAACTCCAC ACAATGACAA AGAAGCAATG 
ACAAGGAAAA TTTTAAACAT TACCGATGTG GTTGCAGCAT GCAGAGATAC TGGATATGAC 
TGGTTTGAGC AACTGCTTCA AAACTTGTTG AAGTCCGAAG AGGATTCCTC ATATAAACCT 
GTGAAGAAAG CTTGTACTCA ACTTGTTGAT AACCTAGTTG AGCACATTCT TAAATATGAG 
GAATCTCTAG CTGACTCTGA CAATAAAGGT GTGAATTCTG GAAGATTGGT AGCTTGCATA 
ACCACTTTGT TCTTATTCAG CAAAATAAGA CCCCAGCTCA TGGTTAAACA TGCAATGACT 
ATGCAACCAT ACCTTACCAC TAAATGTAGT ACGCAAAATG ATTTCATGGT TATCTGCAAT 
GTTGCAAAAA TCCTAGAGCT AGTTGTACCA CTGATGGAGC ATCCAAGTGA AACTTTTCTT 
GCCACTATTG AGGAAGATCT AATGAAGCTC ATCATCAAAT ATGGCATGAC TGTAGTGCAA 
CATTGTGTGA GCTGTCTTGG AGCTGTTGTA AATAAAGTGA CACAAAATTT TAAATTTGTG 
TGGGCTTGTT TCAATAGATA CTATGGTGCC ATTTCAAAAT TAAAAAGTCA ACACCAAGAG 
GACCCAAATA ACACTTCACT TCTAACAAAC AAACCAGCAC TTCTTAGATC CCTTTTCACC 
GTTGGAGCAC TATGTCGGCA TTTTGATTTT GATCTGGAAG ATTTTAAAGG CAACAGCAAG 
GTTAACATAA AAGATAAAGT ACTTGAACTA TTGATGTATT TTACAAAACA CTCAGATGAA 
GAAGTACAAA CAAAAGCTAT CATTGGTCTA GGATTTGCCT TTATTCAGCA TCCAAGTCTA 
ATGTTCGAGC AAGAAGTGAA GAATCTATAT AATAATATTT TATCTGATAA GAACTCCTCA 
GTCAATTTAA AAATACAAGT GTTAAAAAAC CTCCAGACCT ACCTACAAGA AGAAGATACA 
CGTATGCAGC AGGCAGATAG AGACTGGAAG AAAGTTGCAA AACAGGAAGA CTTAAAAGAA 
ATGGGTGATG TTTCCTCAGG GATGAGTAGT TCCATCATGC AGCTTTATCT CAAACAGGTG 
CTTGAGGCAT TTTTTCACAC CCAGTCAAGT GTACGCCACT TTGCCCTAAA TGTCATTGCA 
TTGACTCTAA ATCAAGGTCT TATTCATCCA GTTCAGTGTG TGCCATATTT AATTGCTATG 
GGCACAGACC CAGAACCTGC TATGCGGAAC AAGGCTGATC AGCAACTTGT GGAAATAGAC 
AAAAAATATG CTGGATTCAT TCATATGAAA GCAGTGGCTG GTATGAAGAT GTCTTACCAG 
GTACAACAGG CAATCAACAC ATGCCTAAAA GATCCTGTAA GGGGTTTCAG ACAAGACGAG 
TCCTCTAGCG CTTTGTGTTC ACACCTTTAC TCCATGATCC GTGGAAACCG CCAACACAGA 
CGAGCCTTTC TTATTTCTTT ACTCAACCTC TTTGATGACA CAGCAAAAAC AGACGTGACT 
ATGCTCTTGT ATATAGCAGA CAATCTAGCC TGTTTTCCAT ACCAGACACA GGAAGAGCCG 
TTGTTTATAA TGCATCATAT AGACATTACA CTCTCAGTTT CTGGTAGTAA CCTACTGCAG 
TCATTCAAGG AGTCTATGGT AAAGGACAAA AGGAAAGAGA GAAAATCATC ACCTAGTAAG 
GAAAATGAGT CAAGCGACAG TGAAGAAGAA GTTTCCAGGC CTCGGAAGTC ACGGAAACGT 
GTAGATTCAG ATTCAGATTC AGATTCAGAA GACGATATAA ATTCAGTGAT GAAATGTTTG 
CCAGAAAATT CAGCTCCTTT AATCGAATTT GCAAATGTGT CCCAGGGTAT TTTATTACTT 
CTCATGTTAA AACAACATTT GAAGAATCTT TGTGGATTTT CTGATAGTAA AATTCAGAAG 
TACTCTCCAT CTGAATCTGC AAAAGTATAT GATAAAGCGA TAAACCGAAA AACAGGAGTT 
CATTTTCATC CAAAACAAAC ACTGGACTTC CTGCGGAGTG ACATGGCTAA TTCCAAAATC 
ACAGAAGAGG TGAAAAGGAG TATAGTAAAA CAGTATCTAG ATTTCAAACT TCTCATGGAA 
CATCTGGACC CTGATGAAGA AGAAGAAGAA GGGGAGGTTT CAGCTAGCAC AAATGCTCGG 
AACAAAGCAA TTACCTCACT GCTTGGAGGA GGCAGCCCTA AAAATAATAC AGCAGCAGAG 
ACAGAAGATG ATGAAAGTGA TGGGGAGGAT AGAGGAGGAG GCACTTCAGG GTCATTGAGA 
AGGTCAAAAC GAAATTCAGA CTCTACGGAG TTGGCAGCAC AGATGAATGA AAGTGTTGAC 
GTCATGGATG TCATCGCTAT TTGCTGTCCA AAGTACAAAG ATCGACCACA AATTGCAAGA 
GTAGTGCAGA AAACCAGCAG TGGCTTCAGT GTTCAGTGGA TGGCAGGCTC CTACAGTGGC 
TCCTGGACTG AGGCTAAGCG CCGTGATGGC CGCAAACTGG TGCCTTGGGT AGACACTATT 
AAAGAGTCAG ACATTATTTA CAAAAAAATT GCTCTAACGA GTGCTAATAA GCTGACTAAT 
AAAGTTGTTC AGACTTTACG ATCCCTGTAT GCCGCCAAGG ATGGGACTTC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005248280.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248337.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X3, mRNA.

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CloneID	OHu39308
Clone ID Related Accession (Same CDS sequence)	XM_005248280.3
Accession Version	XM_005248280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248337.1
CDS	489..8585
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_005248280.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248280.3

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_011514015.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512317.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X6, mRNA.

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CloneID	OHu66530
Clone ID Related Accession (Same CDS sequence)	XM_011514015.1
Accession Version	XM_011514015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512317.1
CDS	489..7754
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTA

Target ORF information:

RefSeq Version	XM_011514015.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514015.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_017009329.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864818.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X4, mRNA.

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CloneID	OHu95812
Clone ID Related Accession (Same CDS sequence)	XM_017009329.1
Accession Version	XM_017009329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG 
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA 
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT 
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA 
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA 
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT 
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG 
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC 
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA 
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGGTGAGGCG GAGGAGGAGT 
CAACGTATTT CGCAGCGTAT TACTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864818.1
CDS	489..8438
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTASMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_017009329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009329.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATT ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAGGTG AACTGCCTTT TGGCTTGTAG GGATGACAAT 
TTGGTTTCAC AGCTTGTCCA TAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA CCCAGAAGGT GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCAAGGAGTC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAGCAA AATAGCCCAG TGCCTAGTCC ATACGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTACACAAC ACATCCACAG 
ATGCAACAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CATACATGAT 
AATAAAGTTT CTGGTCCGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGTTCAA GTGAGGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCATCTATGG TAAAGGACAA AAGGAAAGAG AGAAAATCAT CACCTAGTAA GGAAAATGAG 
TCAAGCGACA GTGAAGAAGA AGTTTCCAGG CCTCGGAAGT CACGGAAACG TGTAGATTCA 
GATTCAGATT CAGATTCAGA AGACGATATA AATTCAGTGA TGAAATGTTT GCCAGAAAAT 
TCAGCTCCTT TAATCGAATT TGCAAATGTG TCCCAGGGTA TTTTATTACT TCTCATGTTA 
AAACAACATT TGAAGAATCT TTGTGGATTT TCTGATAGTA AAATTCAGAA GTACTCTCCA 
TCTGAATCTG CAAAAGTATA TGATAAAGCG ATAAACCGAA AAACAGGAGT TCATTTTCAT 
CCAAAACAAA CACTGGACTT CCTGCGGAGT GACATGGCTA ATTCCAAAAT CACAGAAGAG 
GTGAAAAGGA GTATAGTAAA ACAGTATCTA GATTTCAAAC TTCTCATGGA ACATCTGGAC 
CCTGATGAAG AAGAAGAAGA AGGGGAGGTT TCAGCTAGCA CAAATGCTCG GAACAAAGCA 
ATTACCTCAC TGCTTGGAGG AGGCAGCCCT AAAAATAATA CAGCAGCAGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGGTGAGGCG GAGGAGGAGT 
CAACGTATTT CGCAGCGTAT TACTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005248282.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248339.3 Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X5, mRNA.

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CloneID	OHu95813
Clone ID Related Accession (Same CDS sequence)	XM_005248282.4 , XM_005248282.5
Accession Version	XM_005248282.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248282.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248339.3
CDS	651..8405
Translation	MLRLLILLDTISILLRYTKKKTGKCRRIMLLNSNTLPDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_005248282.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248282.4

ORF Insert Sequence:

ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005248282.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248339.3 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X5, mRNA.

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CloneID	OHu95813
Clone ID Related Accession (Same CDS sequence)	XM_005248282.4 , XM_005248282.5
Accession Version	XM_005248282.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248282.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248339.3
CDS	650..8404
Translation	MLRLLILLDTISILLRYTKKKTGKCRRIMLLNSNTLPDGDSSTMRNAASFPLRSPQPVCSPAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_005248282.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248282.5

ORF Insert Sequence:

ATGCTTAGGC TGTTGATTCT TTTGGATACA ATTTCAATTC TTCTAAGATA TACCAAGAAG 
AAAACAGGAA AGTGCAGAAG AATTATGCTT TTGAATTCCA ACACTTTACC TGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCATCTTTT CCCTTGAGAT CTCCACAGCC AGTATGCTCC 
CCTGCTGGAA GTGAAGGAAC TCCTAAAGGC TCAAGACCAC CTTTAATCCT ACAATCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGATA TCTTGCTAGA TTCTCCAGAA 
AGAAAACAAA AGAAGCAGAA GAAAATGAAA TTAGGCAAGG ATGAAAAAGA GCAGAGTGAG 
AAAGCGGCAA TGTATGATAT AATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGGTC TTCAGACATG GACCAGCAAG AGGATATGAT TTCTGGTGTG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAAGATATA AACCGCCCAC TAAATGCTGC TCAATGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTGCAAAACA ACCCCAGACT TCTGTGGTAC AGAATCAACA ACAGATATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGC TTCTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCTTCTAT AACTCAGGAT 
TCAGACTCCA TAAAAAAGCC TGAAGAAATC AAACAATGTA ATGATGCACC TGTTTCTGTT 
CTTCAGGAAG ATATTGTTGG AAGTCTTAAA TCTACACCAG AAAACCATCC TGAGACACCT 
AAAAAAAAGT CTGATCCTGA GCTTTCAAAG AGTGAAATGA AACAAAGTGA AAGTAGATTA 
GCAGAATCTA AACCAAATGA AAACCGATTG GTGGAGACAA AATCAAGTGA AAATAAGTTA 
GAAACTAAAG TTGAGACCCA AACAGAAGAA CTTAAACAGA ATGAGAGCAG AACAACTGAA 
TGCAAACAAA ACGAGAGCAC CATAGTTGAG CCTAAACAAA ATGAAAATAG ACTGTCTGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGGCCT 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGG CCTGAGACTC CAAAACAAAA GAGTGATGGG 
CATCCTGAAA CCCCAAAACA GAAGGGTGAT GGAAGGCCTG AAACTCCAAA GCAAAAAGGT 
GAGAGCCGCC CTGAAACTCC AAAGCAAAAA AATGAAGGGC GACCTGAAAC ACCAAAACAC 
AGGCATGACA ATAGGAGGGA TTCTGGAAAG CCATCTACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AAGATACTAA ATCTGACTCA CCTCGGTTAA AATCAGAACG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCTGTT TCTGAGTCAC TAAGACGTGA CCATGATAAT 
AAACAAAAAT CAGATGACAG GGGTGAATCA GAGCGACATC GAGGGGATCA GTCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGAAC ATGGCATTAA ATCTGATAGT 
TCAAAAACTG ATAAACTAGA ACGAAAACAC AGGCATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA ATCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GATCTGATAA ACTTGGTTTT AAATCACCAA CTAGTAAAGA TGACAAAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACACT AATAAAGCAC ACCCTGACAA TAAGGCAGAA 
TTTCCAAGTT ATTTGTTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCGAAA 
ATCAAGAGGG ATAAAGATGG CAATGTTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCCGAAAG ACAAAGTAGA AAAAATAGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TGCTACAAAA ACTGTCTTTG GATGATGTTC AGAAACTTAT TAAAGATAGA 
GAGGACAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAG 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAACAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAGCCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAGCTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGGAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGT CCGTCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTTGCTAGG AAAATGAAGA AAAAAGAAAA ACAGAAGAAA 
AGGAAAGCAT ATGAACCAAA ACTAACACCT GAAGAAATGA TGGACTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAATTTGG AAGATATGGA TTTTACTGCG 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATAAAAGCA ATGGGTATAA TGGATAAGCT TTCAACTGAC 
AAAACTGTGA AAGTCTTAAA TATCTTGGAG AAGAATATTC AGGATGGGTC AAAGCTTTCC 
ACTTTGTTAA ATCATAATAA CGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TTACAAAATC AGCGGATGCT TGTCTTACAA CTATCAACAT TATGACATCC 
CCTAACATGC CAAAAGCTGT GTACATTGAG GATGTAATTG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTATTAAG TTCAAAAGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAACAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAATTG 
CTAGAGATAC AACTTCTTAC AGACACAACA ATTCTTCAGG TTTCATCTAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAGTTGTGTG CCATTAAGTT AGTCACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTT 
GCAAGATTAC CAACCAGCAA GAGGAGTTTA AGGAACTTCA GGTTAAACAG TAGTGATATG 
GATGGAGAAC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT TATTCAGTGT 
GTGGTACACT TACCATCATC AGAGAAGGAC TCTAATGCAG AAGAAGATTC AAATAAAAAA 
ATTGACCAGG ATGTTGTCAT TACTAACTCT TATGAAACAG CTATGCGAAC AGCCCAAAAC 
TTCCTCTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTGTTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAACAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCTCTT GATTACCTTG GAACTGTTGC TGCACGGCTA 
AGAAAAGATG CTGTTACAAG CAAAATGGAT CAAGGATCTA TAGAACGCAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATCCAACAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACTG AGACTGATCC TTCACTAGTG TTTTCTCGTA AATTCTATAT AGCCCAGTGG 
TTTCGAGACA CAACTCTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAA CACATCATGC AAAGGAAATT GAGACAACTG GCCAAATTAT GCATCGAGCT 
GAAAACCGAA AAAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTTAGCACA 
TTAAAGATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GCTTGATTGT TCGATACTTG 
GCCTCCATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTACGAGTT 
CTTGGTGAAA ATGCAATTGC TGTTCGAACA AAAGCCATGA AGTGTTTGTC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTT GATATGCAAC GAGGTGTTCA TGGACGATTG 
ATGGATAATT CGACTAGTGT CCGAGAAGCA GCAGTAGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA ACAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGGAAAAG AGTAATAAAG ATTCTCAGAG ACATTTGTAT TGAACAACCA 
ACATTTCCAA AAATCACAGA AATGTGTGTA AAAATGATTC GCAGAGTCAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAGAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAAATT TTAAACATTA CCGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAA CTGCTTCAAA ACTTGTTGAA GTCCGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CAATGACTAT GCAACCATAC CTTACCACTA AATGTAGTAC GCAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATC CTAGAGCTAG TTGTACCACT GATGGAGCAT 
CCAAGTGAAA CTTTTCTTGC CACTATTGAG GAAGATCTAA TGAAGCTCAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTGAGC TGTCTTGGAG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCTTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTTC TAACAAACAA ACCAGCACTT 
CTTAGATCCC TTTTCACCGT TGGAGCACTA TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGCAAGGT TAACATAAAA GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAACACT CAGATGAAGA AGTACAAACA AAAGCTATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCATC CAAGTCTAAT GTTCGAGCAA GAAGTGAAGA ATCTATATAA TAATATTTTA 
TCTGATAAGA ACTCCTCAGT CAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA AGTTGCAAAA 
CAGGAAGACT TAAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTATCTCA AACAGGTGCT TGAGGCATTT TTTCACACCC AGTCAAGTGT ACGCCACTTT 
GCCCTAAATG TCATTGCATT GACTCTAAAT CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCATATTTAA TTGCTATGGG CACAGACCCA GAACCTGCTA TGCGGAACAA GGCTGATCAG 
CAACTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ATATGAAAGC AGTGGCTGGT 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACAT GCCTAAAAGA TCCTGTAAGG 
GGTTTCAGAC AAGACGAGTC CTCTAGCGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AACACAGACG AGCCTTTCTT ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG ACGTGACTAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCAGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCTATGGTAA AGGACAAAAG GAAAGAGAGA 
AAATCATCAC CTAGTAAGGA AAATGAGTCA AGCGACAGTG AAGAAGAAGT TTCCAGGCCT 
CGGAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCAG ATTCAGAAGA CGATATAAAT 
TCAGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TCGAATTTGC AAATGTGTCC 
CAGGGTATTT TATTACTTCT CATGTTAAAA CAACATTTGA AGAATCTTTG TGGATTTTCT 
GATAGTAAAA TTCAGAAGTA CTCTCCATCT GAATCTGCAA AAGTATATGA TAAAGCGATA 
AACCGAAAAA CAGGAGTTCA TTTTCATCCA AAACAAACAC TGGACTTCCT GCGGAGTGAC 
ATGGCTAATT CCAAAATCAC AGAAGAGGTG AAAAGGAGTA TAGTAAAACA GTATCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAAG AAGAAGAAGG GGAGGTTTCA 
GCTAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCACTGC TTGGAGGAGG CAGCCCTAAA 
AATAATACAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAT 
CGACCACAAA TTGCAAGAGT AGTGCAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_017009330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864819.1 Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X7, mRNA.

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CloneID	OHu95814
Clone ID Related Accession (Same CDS sequence)	XM_017009330.1 , XM_017009330.2
Accession Version	XM_017009330.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT 
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA 
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA 
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG 
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT 
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT 
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG 
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT 
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA 
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA 
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG 
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA 
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT 
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG 
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT 
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA 
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT 
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA 
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA 
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG 
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA 
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT 
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT 
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT 
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT 
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT 
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT 
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG 
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG 
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT 
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG 
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT 
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT 
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT 
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT 
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT 
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT 
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA 
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC 
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT 
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG 
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA 
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA 
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT 
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA 
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT 
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG 
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA 
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA 
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA 
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA 
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG 
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG 
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA 
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG 
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG 
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA 
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA 
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC 
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC 
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA 
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT 
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA 
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC 
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT 
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA 
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA 
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA 
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA 
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA 
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT 
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT 
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC 
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT 
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG 
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA 
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG 
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA 
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA 
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA 
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT 
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC 
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT 
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC 
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA 
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG 
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC 
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT 
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT 
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT 
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA 
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC 
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC 
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA 
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT 
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG 
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG 
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA 
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG 
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT 
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG 
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT 
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA 
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT 
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT 
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA 
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA 
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA 
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG 
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG 
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG 
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG 
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864819.1
CDS	4380..11177
Translation	MVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_017009330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL, cohesin loading factor (NIPBL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009330.1

ORF Insert Sequence:

ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT 
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA 
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA 
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG 
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT 
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT 
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG 
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT 
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA 
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA 
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG 
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA 
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT 
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG 
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT 
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA 
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT 
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA 
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA 
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG 
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA 
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT 
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT 
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT 
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT 
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT 
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT 
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG 
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG 
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT 
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG 
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT 
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT 
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT 
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT 
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT 
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT 
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA 
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC 
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT 
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG 
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA 
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA 
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT 
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA 
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT 
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG 
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA 
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA 
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA 
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA 
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG 
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG 
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA 
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG 
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG 
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA 
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA 
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC 
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC 
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA 
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT 
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA 
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC 
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT 
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA 
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA 
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA 
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA 
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA 
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT 
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT 
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC 
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT 
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG 
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA 
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG 
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA 
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA 
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA 
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT 
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC 
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT 
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC 
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA 
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG 
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC 
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT 
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT 
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT 
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA 
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC 
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC 
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA 
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT 
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG 
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG 
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA 
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG 
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT 
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG 
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT 
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA 
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT 
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT 
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA 
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA 
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA 
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG 
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG 
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG 
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG 
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_017009330.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864819.1 Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X7, mRNA.

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CloneID	OHu95814
Clone ID Related Accession (Same CDS sequence)	XM_017009330.1 , XM_017009330.2
Accession Version	XM_017009330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nipped-B-like protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT 
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA 
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA 
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG 
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT 
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT 
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG 
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT 
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA 
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA 
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG 
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA 
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT 
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG 
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT 
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA 
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT 
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA 
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA 
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG 
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA 
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT 
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT 
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT 
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT 
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT 
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT 
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG 
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG 
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT 
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG 
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT 
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT 
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT 
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT 
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT 
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT 
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA 
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC 
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT 
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG 
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA 
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA 
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT 
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA 
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT 
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG 
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA 
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA 
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA 
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA 
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG 
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG 
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA 
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG 
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG 
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA 
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA 
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC 
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC 
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA 
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT 
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA 
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC 
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT 
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA 
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA 
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA 
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA 
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA 
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT 
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT 
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC 
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT 
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG 
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA 
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG 
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA 
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA 
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA 
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT 
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC 
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT 
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC 
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA 
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG 
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC 
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT 
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT 
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT 
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA 
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC 
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC 
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA 
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT 
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG 
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG 
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA 
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG 
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT 
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG 
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT 
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA 
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT 
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT 
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA 
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA 
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA 
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG 
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG 
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG 
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG 
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864819.1
CDS	1731..8528
Translation	MVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRLVETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGKPSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRVRRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGFKSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKGEPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_017009330.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIPBL cohesin loading factor (NIPBL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009330.2

ORF Insert Sequence:

ATGGTTAGCA TTGATCTTCA TCAGGCAGGA AGAGTGGACT CTCAGGCTTC TATAACTCAG 
GATTCAGACT CCATAAAAAA GCCTGAAGAA ATCAAACAAT GTAATGATGC ACCTGTTTCT 
GTTCTTCAGG AAGATATTGT TGGAAGTCTT AAATCTACAC CAGAAAACCA TCCTGAGACA 
CCTAAAAAAA AGTCTGATCC TGAGCTTTCA AAGAGTGAAA TGAAACAAAG TGAAAGTAGA 
TTAGCAGAAT CTAAACCAAA TGAAAACCGA TTGGTGGAGA CAAAATCAAG TGAAAATAAG 
TTAGAAACTA AAGTTGAGAC CCAAACAGAA GAACTTAAAC AGAATGAGAG CAGAACAACT 
GAATGCAAAC AAAACGAGAG CACCATAGTT GAGCCTAAAC AAAATGAAAA TAGACTGTCT 
GACACAAAAC CAAATGACAA CAAACAAAAT AATGGCAGAT CAGAAACAAC AAAATCAAGG 
CCTGAAACCC CAAAGCAAAA GGGTGAAAGC CGGCCTGAGA CTCCAAAACA AAAGAGTGAT 
GGGCATCCTG AAACCCCAAA ACAGAAGGGT GATGGAAGGC CTGAAACTCC AAAGCAAAAA 
GGTGAGAGCC GCCCTGAAAC TCCAAAGCAA AAAAATGAAG GGCGACCTGA AACACCAAAA 
CACAGGCATG ACAATAGGAG GGATTCTGGA AAGCCATCTA CAGAGAAAAA ACCTGAAGTG 
TCTAAACATA AACAAGATAC TAAATCTGAC TCACCTCGGT TAAAATCAGA ACGAGCTGAA 
GCCTTAAAGC AGAGACCTGA TGGGCGATCT GTTTCTGAGT CACTAAGACG TGACCATGAT 
AATAAACAAA AATCAGATGA CAGGGGTGAA TCAGAGCGAC ATCGAGGGGA TCAGTCTAGG 
GTTCGAAGAC CAGAAACATT GAGATCCTCT AGTAGAAATG AACATGGCAT TAAATCTGAT 
AGTTCAAAAA CTGATAAACT AGAACGAAAA CACAGGCATG AATCAGGGGA CTCAAGGGAA 
AGACCATCTT CTGGGGAACA AAAATCAAGA CCTGACAGTC CTCGTGTTAA ACAAGGAGAT 
TCTAATAAAT CAAGATCTGA TAAACTTGGT TTTAAATCAC CAACTAGTAA AGATGACAAA 
AGGACAGAGG GTAACAAGAG TAAAGTAGAC ACTAATAAAG CACACCCTGA CAATAAGGCA 
GAATTTCCAA GTTATTTGTT GGGGGGCAGG TCTGGTGCGT TGAAAAATTT TGTCATTCCG 
AAAATCAAGA GGGATAAAGA TGGCAATGTT ACTCAGGAGA CAAAGAAAAT GGAAATGAAA 
GGAGAGCCGA AAGACAAAGT AGAAAAAATA GGATTAGTTG AAGATCTAAA TAAAGGAGCT 
AAGCCTGTAG TTGTGCTACA AAAACTGTCT TTGGATGATG TTCAGAAACT TATTAAAGAT 
AGAGAGGACA AATCAAGAAG TTCCCTTAAA CCTATCAAGA ATAAACCATC AAAGTCAAAT 
AAAGGTAGTA TAGATCAATC AGTGTTAAAA GAATTACCCC CTGAACTCCT GGCAGAAATT 
GAGTCCACCA TGCCACTTTG TGAACGTGTG AAAATGAACA AACGCAAGCG TAGCACAGTT 
AATGAAAAGC CAAAATATGC TGAAATCAGT TCAGATGAAG ATAATGATAG TGATGAAGCT 
TTTGAATCCT CTAGGAAACG ACATAAAAAA GATGATGATA AAGCTTGGGA ATATGAAGAG 
CGTGACAGAA GAAGCTCTGG GGATCATAGG AGAAGTGGCC ACTCTCATGA AGGAAGAAGG 
AGTTCAGGTG GTGGTCGTTA TCGAAACCGA AGTCCGTCAG ATTCTGACAT GGAAGATTAT 
TCTCCTCCTC CCAGCCTTAG TGAGGTTGCT AGGAAAATGA AGAAAAAAGA AAAACAGAAG 
AAAAGGAAAG CATATGAACC AAAACTAACA CCTGAAGAAA TGATGGACTC TTCAACTTTT 
AAGAGATTCA CAGCCTCAAT AGAGAATATT TTGGATAATT TGGAAGATAT GGATTTTACT 
GCGTTTGGTG ATGATGATGA AATTCCTCAG GAACTGCTCT TAGGAAAACA TCAGCTTAAT 
GAACTTGGCA GTGAATCTGC TAAAATAAAA GCAATGGGTA TAATGGATAA GCTTTCAACT 
GACAAAACTG TGAAAGTCTT AAATATCTTG GAGAAGAATA TTCAGGATGG GTCAAAGCTT 
TCCACTTTGT TAAATCATAA TAACGATACT GAAGAAGAAG AAAGGTTATG GAGAGACCTT 
ATTATGGAGA GAGTTACAAA ATCAGCGGAT GCTTGTCTTA CAACTATCAA CATTATGACA 
TCCCCTAACA TGCCAAAAGC TGTGTACATT GAGGATGTAA TTGAAAGAGT TATACAGTAC 
ACTAAATTTC ATTTGCAGAA TACACTTTAT CCTCAGTATG ATCCTGTTTA CAGATTAGAT 
CCTCATGGAG GAGGCTTATT AAGTTCAAAA GCAAAACGGG CTAAATGTTC TACCCATAAG 
CAGAGAGTAA TAGTAATGCT TTATAACAAA GTTTGTGACA TTGTTAGCAG CTTATCAGAA 
TTGCTAGAGA TACAACTTCT TACAGACACA ACAATTCTTC AGGTTTCATC TATGGGAATA 
ACACCATTTT TTGTGGAAAA TGTCAGTGAA CTACAGTTGT GTGCCATTAA GTTAGTCACT 
GCAGTATTCT CAAGATATGA AAAACATAGG CAGTTAATTT TGGAAGAAAT TTTTACTTCA 
CTTGCAAGAT TACCAACCAG CAAGAGGAGT TTAAGGAACT TCAGGTTAAA CAGTAGTGAT 
ATGGATGGAG AACCTATGTA TATTCAGATG GTTACAGCAC TGGTTTTACA ACTTATTCAG 
TGTGTGGTAC ACTTACCATC ATCAGAGAAG GACTCTAATG CAGAAGAAGA TTCAAATAAA 
AAAATTGACC AGGATGTTGT CATTACTAAC TCTTATGAAA CAGCTATGCG AACAGCCCAA 
AACTTCCTCT CCATCTTCCT TAAAAAATGT GGTAGTAAGC AAGGTGAAGA AGATTACAGA 
CCACTGTTTG AAAATTTTGT TCAAGACCTT CTTTCAACAG TCAATAAGCC TGAATGGCCA 
GCTGCTGAAC TACTCCTTAG TTTGTTAGGG AGACTGTTGG TTCATCAGTT CAGTAACAAG 
TCAACAGAGA TGGCTTTAAG AGTGGCATCT CTTGATTACC TTGGAACTGT TGCTGCACGG 
CTAAGAAAAG ATGCTGTTAC AAGCAAAATG GATCAAGGAT CTATAGAACG CATTTTAAAA 
CAGGTTTCAG GAGGGGAAGA TGAAATCCAA CAATTACAAA AAGCATTGCT TGATTACTTG 
GATGAAAACA CTGAGACTGA TCCTTCACTA GTGTTTTCTC GTAAATTCTA TATAGCCCAG 
TGGTTTCGAG ACACAACTCT GGAAACAGAA AAAGCAATGA AATCACAAAA AGATGAAGAA 
TCATCTGAAG GAACACATCA TGCAAAGGAA ATTGAGACAA CTGGCCAAAT TATGCATCGA 
GCTGAAAACC GAAAAAAGTT TCTTAGAAGC ATTATCAAAA CCACACCTTC TCAGTTTAGC 
ACATTAAAGA TGAACTCTGA TACTGTGGAC TATGATGATG CTTGCTTGAT TGTTCGATAC 
TTGGCCTCCA TGAGGCCGTT TGCCCAGAGC TTTGATATTT ATTTGACACA GATCCTACGA 
GTTCTTGGTG AAAATGCAAT TGCTGTTCGA ACAAAAGCCA TGAAGTGTTT GTCTGAGGTT 
GTTGCTGTAG ACCCCAGTAT TCTAGCAAGG CTTGATATGC AACGAGGTGT TCATGGACGA 
TTGATGGATA ATTCGACTAG TGTCCGAGAA GCAGCAGTAG AATTACTAGG TCGATTTGTC 
CTTTGTCGAC CTCAGCTTGC TGAACAGTAT TATGATATGC TGATTGAAAG AATATTGGAT 
ACTGGTATCA GTGTCAGGAA AAGAGTAATA AAGATTCTCA GAGACATTTG TATTGAACAA 
CCAACATTTC CAAAAATCAC AGAAATGTGT GTAAAAATGA TTCGCAGAGT CAATGATGAA 
GAGGGCATTA AGAAATTAGT AAATGAAACA TTCCAGAAAC TCTGGTTTAC TCCAACTCCA 
CACAATGACA AAGAAGCAAT GACAAGGAAA ATTTTAAACA TTACCGATGT GGTTGCAGCA 
TGCAGAGATA CTGGATATGA CTGGTTTGAG CAACTGCTTC AAAACTTGTT GAAGTCCGAA 
GAGGATTCCT CATATAAACC TGTGAAGAAA GCTTGTACTC AACTTGTTGA TAACCTAGTT 
GAGCACATTC TTAAATATGA GGAATCTCTA GCTGACTCTG ACAATAAAGG TGTGAATTCT 
GGAAGATTGG TAGCTTGCAT AACCACTTTG TTCTTATTCA GCAAAATAAG ACCCCAGCTC 
ATGGTTAAAC ATGCAATGAC TATGCAACCA TACCTTACCA CTAAATGTAG TACGCAAAAT 
GATTTCATGG TTATCTGCAA TGTTGCAAAA ATCCTAGAGC TAGTTGTACC ACTGATGGAG 
CATCCAAGTG AAACTTTTCT TGCCACTATT GAGGAAGATC TAATGAAGCT CATCATCAAA 
TATGGCATGA CTGTAGTGCA ACATTGTGTG AGCTGTCTTG GAGCTGTTGT AAATAAAGTG 
ACACAAAATT TTAAATTTGT GTGGGCTTGT TTCAATAGAT ACTATGGTGC CATTTCAAAA 
TTAAAAAGTC AACACCAAGA GGACCCAAAT AACACTTCAC TTCTAACAAA CAAACCAGCA 
CTTCTTAGAT CCCTTTTCAC CGTTGGAGCA CTATGTCGGC ATTTTGATTT TGATCTGGAA 
GATTTTAAAG GCAACAGCAA GGTTAACATA AAAGATAAAG TACTTGAACT ATTGATGTAT 
TTTACAAAAC ACTCAGATGA AGAAGTACAA ACAAAAGCTA TCATTGGTCT AGGATTTGCC 
TTTATTCAGC ATCCAAGTCT AATGTTCGAG CAAGAAGTGA AGAATCTATA TAATAATATT 
TTATCTGATA AGAACTCCTC AGTCAATTTA AAAATACAAG TGTTAAAAAA CCTCCAGACC 
TACCTACAAG AAGAAGATAC ACGTATGCAG CAGGCAGATA GAGACTGGAA GAAAGTTGCA 
AAACAGGAAG ACTTAAAAGA AATGGGTGAT GTTTCCTCAG GGATGAGTAG TTCCATCATG 
CAGCTTTATC TCAAACAGGT GCTTGAGGCA TTTTTTCACA CCCAGTCAAG TGTACGCCAC 
TTTGCCCTAA ATGTCATTGC ATTGACTCTA AATCAAGGTC TTATTCATCC AGTTCAGTGT 
GTGCCATATT TAATTGCTAT GGGCACAGAC CCAGAACCTG CTATGCGGAA CAAGGCTGAT 
CAGCAACTTG TGGAAATAGA CAAAAAATAT GCTGGATTCA TTCATATGAA AGCAGTGGCT 
GGTATGAAGA TGTCTTACCA GGTACAACAG GCAATCAACA CATGCCTAAA AGATCCTGTA 
AGGGGTTTCA GACAAGACGA GTCCTCTAGC GCTTTGTGTT CACACCTTTA CTCCATGATC 
CGTGGAAACC GCCAACACAG ACGAGCCTTT CTTATTTCTT TACTCAACCT CTTTGATGAC 
ACAGCAAAAA CAGACGTGAC TATGCTCTTG TATATAGCAG ACAATCTAGC CTGTTTTCCA 
TACCAGACAC AGGAAGAGCC GTTGTTTATA ATGCATCATA TAGACATTAC ACTCTCAGTT 
TCTGGTAGTA ACCTACTGCA GTCATTCAAG GAGTCTATGG TAAAGGACAA AAGGAAAGAG 
AGAAAATCAT CACCTAGTAA GGAAAATGAG TCAAGCGACA GTGAAGAAGA AGTTTCCAGG 
CCTCGGAAGT CACGGAAACG TGTAGATTCA GATTCAGATT CAGATTCAGA AGACGATATA 
AATTCAGTGA TGAAATGTTT GCCAGAAAAT TCAGCTCCTT TAATCGAATT TGCAAATGTG 
TCCCAGGGTA TTTTATTACT TCTCATGTTA AAACAACATT TGAAGAATCT TTGTGGATTT 
TCTGATAGTA AAATTCAGAA GTACTCTCCA TCTGAATCTG CAAAAGTATA TGATAAAGCG 
ATAAACCGAA AAACAGGAGT TCATTTTCAT CCAAAACAAA CACTGGACTT CCTGCGGAGT 
GACATGGCTA ATTCCAAAAT CACAGAAGAG GTGAAAAGGA GTATAGTAAA ACAGTATCTA 
GATTTCAAAC TTCTCATGGA ACATCTGGAC CCTGATGAAG AAGAAGAAGA AGGGGAGGTT 
TCAGCTAGCA CAAATGCTCG GAACAAAGCA ATTACCTCAC TGCTTGGAGG AGGCAGCCCT 
AAAAATAATA CAGCAGCAGA GACAGAAGAT GATGAAAGTG ATGGGGAGGA TAGAGGAGGA 
GGCACTTCAG GGTCATTGAG AAGGTCAAAA CGAAATTCAG ACTCTACGGA GTTGGCAGCA 
CAGATGAATG AAAGTGTTGA CGTCATGGAT GTCATCGCTA TTTGCTGTCC AAAGTACAAA 
GATCGACCAC AAATTGCAAG AGTAGTGCAG AAAACCAGCA GTGGCTTCAG TGTTCAGTGG 
ATGGCAGGCT CCTACAGTGG CTCCTGGACT GAGGCTAAGC GCCGTGATGG CCGCAAACTG 
GTGCCTTGGG TAGACACTAT TAAAGAGTCA GACATTATTT ACAAAAAAAT TGCTCTAACG 
AGTGCTAATA AGCTGACTAA TAAAGTTGTT CAGACTTTAC GATCCCTGTA TGCCGCCAAG 
GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NIPBL
Entrez Gene ID	25836
Full Name	NIPBL, cohesin loading factor
Synonyms	CDLS,CDLS1,IDN3,IDN3-B,Scc2
General protein information	Preferred Names NIPBL, cohesin loading factor Names nipped-B-like protein Nipped-B homolog SCC2 homolog delangin sister chromatid cohesion 2 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p13.2
Summary	This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and cognitive disability. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	608667
Disorder Html:	Cornelia de Lange syndrome 1, 122470 (3)

	NIPBL	XP_001096575.1
Rattus norvegicus (Norway rat)	Nipbl	XP_003749266.1
Drosophila melanogaster (fruit fly)	Nipped-B	NP_001163052.1
Homo sapiens (human)	NIPBL	NP_597677.2
Canis lupus familiaris (dog)	NIPBL	XP_005619439.1
Bos taurus (cattle)	NIPBL	NP_001193513.1
Mus musculus (house mouse)	Nipbl	NP_081983.2
Gallus gallus (chicken)	LOC427025	NP_001244277.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009290	XP_320088.4
Pan troglodytes (chimpanzee)	NIPBL	XP_001146090.1
Gallus gallus (chicken)	LOC427439	XP_425012.3
Danio rerio (zebrafish)	nipblb	NP_001154919.2
Xenopus tropicalis (tropical clawed frog)	nipbl	XP_002938229.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NIPBL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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