BoLA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BoLA
Entrez Gene ID	533050
Full Name	major histocompatibility complex, class I, A
Synonyms	BoLA-NC,HD15
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114855.1	NP_001108327.1	major histocompatibility complex, class I, A precursor
XM_015459890.1	XP_015315376.1	major histocompatibility complex, class I, A isoform X2
XM_024983641.1	XP_024839409.1	major histocompatibility complex, class I, A isoform X3
XM_015459888.1	XP_015315374.1	major histocompatibility complex, class I, A isoform X1
XM_015459888.2	XP_015315374.1	major histocompatibility complex, class I, A isoform X1
XM_015459890.2	XP_015315376.1	major histocompatibility complex, class I, A isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A novel RNAseq-assisted method for MHC class I genotyping in a non-model species applied to a lethal vaccination-induced alloimmune disease.
Demasius W, Weikard R, Hadlich F, Buitkamp J, K?hn C
BMC genomics17365(2016 05)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells.
Davies CJ, Eldridge JA, Fisher PJ, Schlafer DH
American journal of reproductive immunology (New York, N.Y. : 1989)55(3)188-200(2006 Mar)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

cDNA sequence of cattle MHC class I genes transcribed in serologically defined haplotypes A18 and A31.
Ellis SA, Staines KA, Morrison WI
Immunogenetics43(3)156-9(1996)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BoLA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BoLA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa02068	NM_001114855.1 Latest version!	Bos taurus major histocompatibility complex, class I, A (BoLA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa229511	XM_024983641.1 Latest version!	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa88194	XM_015459888.2 Latest version!	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa88195	XM_015459890.2 Latest version!	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OBa88194	XM_015459888.1	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa88195	XM_015459890.1	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BoLA ( NM_001114855.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001108327.1 Bos taurus major histocompatibility complex, class I, A (BoLA), mRNA.

Price & Availability↑

CloneID	OBa02068
Clone ID Related Accession (Same CDS sequence)	NM_001114855.1
Accession Version	NM_001114855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134573.1. On Aug 18, 2008 this sequence version replaced XM_001255612.2. ##Evidence-Data-START## Transcript exon combination :: BC134573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GACCAGGGCT CTGATGTGCC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108327.1
CDS	31..1122
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWEPPQPSIPIMGIIVGLVHLMVTGAMVTGAVIWRKKHSGEKGRIYSQASSSDSDQGSDVPLTVPKV

Target ORF information:

RefSeq Version	NM_001114855.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114855.1

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GACCAGGGCT CTGATGTGCC TCTCACGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BoLA ( XM_024983641.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839409.1 Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa229511
Clone ID Related Accession (Same CDS sequence)	XM_024983641.1
Accession Version	XM_024983641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGGTGAA 
AAAGGACGGA TCTACAGCCA GGCTTCAAGC AGTGACAGTG ACCAGGGCTC TGATGTGCCT 
CTCACGGTTC CTAAAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839409.1
CDS	138..1118
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWGEKGRIYSQASSSDSDQGSDVPLTVPKV

Target ORF information:

RefSeq Version	XM_024983641.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983641.1

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGGTGAA 
AAAGGACGGA TCTACAGCCA GGCTTCAAGC AGTGACAGTG ACCAGGGCTC TGATGTGCCT 
CTCACGGTTC CTAAAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BoLA ( XM_015459888.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315374.1 Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88194
Clone ID Related Accession (Same CDS sequence)	XM_015459888.1 , XM_015459888.2
Accession Version	XM_015459888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315374.1
CDS	29..1120
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWEPPQPSIPIMGIIVGLVHLMVTGAMVTGAVIWRKKHSGEKGRIYSQASSSDSAQGSDVSLMVPKV

Target ORF information:

RefSeq Version	XM_015459888.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459888.1

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BoLA ( XM_015459888.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315374.1 Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88194
Clone ID Related Accession (Same CDS sequence)	XM_015459888.1 , XM_015459888.2
Accession Version	XM_015459888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315374.1
CDS	138..1229
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWEPPQPSIPIMGIIVGLVHLMVTGAMVTGAVIWRKKHSGEKGRIYSQASSSDSAQGSDVSLMVPKV

Target ORF information:

RefSeq Version	XM_015459888.2
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459888.2

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTGA AAAAGGACGG 
ATCTACAGCC AGGCTTCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BoLA ( XM_015459890.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315376.1 Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88195
Clone ID Related Accession (Same CDS sequence)	XM_015459890.1 , XM_015459890.2
Accession Version	XM_015459890.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTCA AACAGGAAGG 
GGCTACACCC AGGCTGCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315376.1
CDS	29..1120
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWEPPQPSIPIMGIIVGLVHLMVTGAMVTGAVIWRKKHSGQTGRGYTQAASSDSAQGSDVSLMVPKV

Target ORF information:

RefSeq Version	XM_015459890.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459890.1

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTCA AACAGGAAGG 
GGCTACACCC AGGCTGCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BoLA ( XM_015459890.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315376.1 Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88195
Clone ID Related Accession (Same CDS sequence)	XM_015459890.1 , XM_015459890.2
Accession Version	XM_015459890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class I, A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTCA AACAGGAAGG 
GGCTACACCC AGGCTGCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315376.1
CDS	139..1230
Translation	MRVVGQRTLLLLLLLDALILTETRAGSHSLRYFYTAVSRPGLGEPRFISVGYVDDTQFVRFDSDAPDPRIEPTARWVEQEGPEYWHQETQRTKDTAQFFRVYLNTLRGYYNQSEAGSHTVQEMYGCDVGPDGLLRGYDQFAYDGRDYIALNEDLRSWTAADTAAQVTKHNAEAAGDAARVRIYLEGKCVEWLRRYLVTGKDTLLRADPPKTHVAHHPISDREVTLRCWALGFYPEEISLTWQRDGEDQTQDMELVETRPSGDGTFQKWAALVVPSGEEQRYTCHVQHEGLQEPLTLRWEPPQPSIPIMGIIVGLVHLMVTGAMVTGAVIWRKKHSGQTGRGYTQAASSDSAQGSDVSLMVPKV

Target ORF information:

RefSeq Version	XM_015459890.2
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class I, A (BoLA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459890.2

ORF Insert Sequence:

ATGCGGGTCG TGGGGCAGAG AACTCTCCTC CTGCTGCTGC TGCTGGATGC GCTGATCCTC 
ACTGAGACCA GGGCGGGGTC CCACTCCCTG AGGTATTTCT ACACCGCCGT GTCCCGGCCC 
GGCCTCGGGG AGCCCCGCTT CATCTCCGTC GGCTACGTGG ACGACACGCA GTTCGTGCGG 
TTCGACAGCG ACGCCCCGGA TCCGAGGATA GAGCCTACAG CGCGGTGGGT GGAGCAGGAG 
GGGCCCGAGT ACTGGCATCA GGAGACGCAG AGAACTAAGG ACACCGCACA ATTTTTCCGA 
GTGTACCTGA ACACCCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACCGTC 
CAAGAGATGT ACGGCTGCGA CGTGGGGCCG GACGGGCTTC TCCGCGGGTA TGATCAGTTC 
GCCTACGACG GCAGAGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCGGCG 
GACACGGCGG CTCAAGTCAC CAAGCACAAT GCTGAGGCGG CAGGTGATGC GGCGCGTGTG 
AGGATCTACC TGGAGGGCAA GTGTGTGGAG TGGCTCCGCA GATACCTGGT GACCGGGAAG 
GACACGCTGC TGCGCGCAGA CCCTCCAAAG ACACATGTGG CCCATCACCC CATCTCTGAC 
CGTGAGGTCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGAGGAGAT CTCACTGACC 
TGGCAGCGTG ACGGGGAGGA CCAGACTCAG GACATGGAGC TTGTGGAGAC CAGGCCTTCA 
GGGGATGGAA CCTTCCAGAA GTGGGCAGCC CTGGTGGTGC CTTCTGGAGA AGAGCAGAGA 
TACACGTGCC ATGTGCAGCA CGAGGGGCTA CAGGAGCCCC TCACCCTGAG ATGGGAACCT 
CCTCAACCCT CCATCCCCAT CATGGGCATC ATTGTTGGAC TGGTTCACCT CATGGTCACT 
GGAGCTATGG TGACTGGAGC TGTGATTTGG AGGAAGAAGC ACTCAGGTCA AACAGGAAGG 
GGCTACACCC AGGCTGCAAG CAGTGACAGT GCCCAGGGCT CTGATGTGTC TCTCATGGTT 
CCTAAAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel RNAseq-assisted method for MHC class I genotyping in a non-model species applied to a lethal vaccination-induced alloimmune disease.
BMC genomics17365(2016 05)
Demasius W,Weikard R,Hadlich F,Buitkamp J,K?hn C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells.
American journal of reproductive immunology (New York, N.Y. : 1989)55(3)188-200(2006 Mar)
Davies CJ,Eldridge JA,Fisher PJ,Schlafer DH

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

cDNA sequence of cattle MHC class I genes transcribed in serologically defined haplotypes A18 and A31.
Immunogenetics43(3)156-9(1996)
Ellis SA,Staines KA,Morrison WI

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BoLA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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