ANO1 cDNA ORF clone, Bos taurus(cattle)

The following ANO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa232027 XM_024987205.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa232025 XM_024987197.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa232026 XM_024987199.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa39758 NM_001192717.1
Latest version!
Bos taurus anoctamin 1 (ANO1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa52105 XM_024987196.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52107 XM_015461360.2
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52109 XM_024987198.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52110 XM_024987200.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52111 XM_015461361.2
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52112 XM_024987202.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52113 XM_015461362.2
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa52114 XM_024987204.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa52115 XM_024987203.1
Latest version!
Bos taurus anoctamin 1 (ANO1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89163 XM_015461360.1
Latest version!
Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89164 XM_015461361.1
Latest version!
Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa89165 XM_015461362.1
Latest version!
Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa232027
    Clone ID Related Accession (Same CDS sequence) XM_024987205.1
    Accession Version XM_024987205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTGGGCA CAGTGGGCTC AGCCTGCCTC TGGCCCCACC AGTCAGCTCA TACACCGATG 
    AGATGGCCCT GGAGCGGGGC CCCCGCCCTC CCGCCCGCTC GCACAACAAA AATTCACGGG
    GTTGGATTTG TAAAAATCCA CGCACCCTGG AATGTGCTGT GCCGAGAAGC CGAGTTTTTG
    AAGCTGAAGA TGCCGACAAA GAAGCTGTAT CACATCAGCG AGACCCGCGG CCTCCTCAAG
    AAGATCAACT CTGTGCTCCA GAAGATCACG GACCCCATCC AGCCCAAGGT GGCAGAGCAC
    AGGCCCCAGA CCACCAAGAG GCTCTCCTAC CCCTTTTCCC GGGAGAAGCA GCACCTATTT
    GACCTGTCTG ACAAGGATTC CTTTTTCGAC AGCAAAACTC GGAGCACAAT AGTCTACGAA
    ATCCTGAAGA GAACAACGTG TACGAAGGCC AAGTACAGCA TGGGCATCAC GAGCCTGCTG
    GCCAATGGTG TCTACTCGGC GGCCTACCCG CTGCATGATG GAGACTATGA AGGAGACAAC
    GTGGAGTTCA ATGACAGGAA GCTCCTGTAC GAAGAGTGGG CGAGTTACGG GGTCTTCTAC
    AAGTACCAGC CCATCGACCT CGTCAGAAAG TATTTTGGGG AGAAGATTGG TCTGTACTTC
    GCCTGGCTTG GCGTCTACAC CCAGATGCTC ATCCCGGCCT CCGTGGTCGG CATCATCGTT
    TTCCTCTACG GATGGGCCAC TGTCGATGAC AACATCCCCA GCATGGAGAT GTGCGACCAG
    AGGCACAACA TCACCATGTG CCCGCTCTGC GACAAGACCT GCAGCTACTG GAAGATGAGC
    TCCGCGTGCG CCACCGCCCG CGCCAGCCAC CTCTTCGACA ACCCCGCCAC TGTCTTCTTC
    TCCATCTTCA TGGCCCTCTG GGCTGCCACC TTCATGGAGC ACTGGAAGCG GAAACAGATG
    CGGCTCAATT ACCGGTGGGA CCTCACAGGC TTCGAGGAGG AGGAGGACCA TCCCAGAGCT
    GAATATGAAG CCCGAGTTCT GGAGAAGTCT CTTAGGAAAG AATCCAAAAA CAAAGAGACA
    GACAAGGTGA AGCTGACCTG GAGAGACCGG TTCCCTGCCT ACTTCACCAA TTTGGTGTCT
    ATCATCTTCA TGATCGCCGT GACCTTTGCC ATTGTCCTTG GCGTCATCAT CTACAGAATC
    TCCACGGCCG CCGCCTTGGC CATGAACTCC TCCCCGTCCG TGCGGTCCAA CATCCGGGTC
    ACGGTCACAG CCACCGCGGT CATCATCAAC CTGGTGGTCA TCATTCTCCT GGACGAGGTG
    TACGGCTGCA TAGCCAGATG GCTCACCAAG ATAGAGGTTC CAAAGACGGA GAAGAGCTTT
    GAGGAGAGGC TGATCTTCAA GGCTTTCCTG CTCAAGTTCG TCAACTCCTA CACACCCATC
    TTCTACGTGG CTTTCTTCAA AGGCCGGTTT GTCGGACGCC CGGGTGACTA CGTGTACATT
    TTCCGATCTT TCCGGATGGA GGAGTGCGCC CCGGGGGGCT GCCTGATGGA GCTCTGCATC
    CAGCTCAGCA TCATCATGCT GGGAAAGCAG CTGATCCAGA ACAACCTGTT TGAGATCGGC
    ATCCCGAAAA TGAAGAAGTT CATCCGCTAT CTGAGGCTGA AGCACCAGAG CCCCTCGGAC
    CACGACGAGC ACGTGAAGAA GAAGCAGCGG TACGAGGTGG ACTACACGCT GGAGCCCTTC
    GCGGGCCTCA CCCCGGAGTA CATGGAGATG ATCATCCAGT TCGGCTTCGT CACCCTGTTT
    GTCGCATCCT TCCCGCTGGC CCCACTCTTC GCGCTCCTGA ACAACATCAT TGAGATCCGC
    CTGGACGCCA AAAAGTTTGT CACCGAGCTC CGAAGGCCGG TGGCTGTCAG AGCCAAGGAC
    ATCGGAATCT GGTACAATAT CCTCAGAGGC GTCGGGAAGC TTGCCGTCAT CATCAACGCG
    TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT
    GAGAACGGGA CCATGCATGG CTTCGTCAAC CACACCCTCT CTTCCTTCAA CGTCAGCGAC
    TTCCAGAACG GCACGGCCCC CAACGACCCC CTGGATCTGG GCTACGAGGT GCAGATCTGC
    AGGTACAAAG ACTACCGGGA GCCGCCATGG TCGGAACACA AGTATGACAT CTCCAAGGAC
    TTCTGGGCCG TCCTGGCCGC CCGGCTGGCC TTTGTCATCG TCTTCCAGAA CCTGGTCATG
    TTCATGAGCG ACTTTGTGGA CTGGGTCATC CCCGACATCC CCAAGGACAT CAGCCAGCAG
    ATCCACAAAG AGAAGGTGCT CATGGTGGAG CTGTTCATGC GGGAGGAGCA GGGCAAGCAG
    CAGCTGCTGG ACACGTGGAT GGAGAAGGAC CGGAAGAAGG CGGAGCCCTG CAACAACCAC
    AGCCCCGCCC CAGCCGCCCC GCCCCTGCCC CCGCCCCCGG AAGGCCCCGA CTTCCCCGGA
    GGCTCCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842973.1
    CDS263..2794
    Translation

    Target ORF information:

    RefSeq Version XM_024987205.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTGGGCA CAGTGGGCTC AGCCTGCCTC TGGCCCCACC AGTCAGCTCA TACACCGATG 
    AGATGGCCCT GGAGCGGGGC CCCCGCCCTC CCGCCCGCTC GCACAACAAA AATTCACGGG
    GTTGGATTTG TAAAAATCCA CGCACCCTGG AATGTGCTGT GCCGAGAAGC CGAGTTTTTG
    AAGCTGAAGA TGCCGACAAA GAAGCTGTAT CACATCAGCG AGACCCGCGG CCTCCTCAAG
    AAGATCAACT CTGTGCTCCA GAAGATCACG GACCCCATCC AGCCCAAGGT GGCAGAGCAC
    AGGCCCCAGA CCACCAAGAG GCTCTCCTAC CCCTTTTCCC GGGAGAAGCA GCACCTATTT
    GACCTGTCTG ACAAGGATTC CTTTTTCGAC AGCAAAACTC GGAGCACAAT AGTCTACGAA
    ATCCTGAAGA GAACAACGTG TACGAAGGCC AAGTACAGCA TGGGCATCAC GAGCCTGCTG
    GCCAATGGTG TCTACTCGGC GGCCTACCCG CTGCATGATG GAGACTATGA AGGAGACAAC
    GTGGAGTTCA ATGACAGGAA GCTCCTGTAC GAAGAGTGGG CGAGTTACGG GGTCTTCTAC
    AAGTACCAGC CCATCGACCT CGTCAGAAAG TATTTTGGGG AGAAGATTGG TCTGTACTTC
    GCCTGGCTTG GCGTCTACAC CCAGATGCTC ATCCCGGCCT CCGTGGTCGG CATCATCGTT
    TTCCTCTACG GATGGGCCAC TGTCGATGAC AACATCCCCA GCATGGAGAT GTGCGACCAG
    AGGCACAACA TCACCATGTG CCCGCTCTGC GACAAGACCT GCAGCTACTG GAAGATGAGC
    TCCGCGTGCG CCACCGCCCG CGCCAGCCAC CTCTTCGACA ACCCCGCCAC TGTCTTCTTC
    TCCATCTTCA TGGCCCTCTG GGCTGCCACC TTCATGGAGC ACTGGAAGCG GAAACAGATG
    CGGCTCAATT ACCGGTGGGA CCTCACAGGC TTCGAGGAGG AGGAGGACCA TCCCAGAGCT
    GAATATGAAG CCCGAGTTCT GGAGAAGTCT CTTAGGAAAG AATCCAAAAA CAAAGAGACA
    GACAAGGTGA AGCTGACCTG GAGAGACCGG TTCCCTGCCT ACTTCACCAA TTTGGTGTCT
    ATCATCTTCA TGATCGCCGT GACCTTTGCC ATTGTCCTTG GCGTCATCAT CTACAGAATC
    TCCACGGCCG CCGCCTTGGC CATGAACTCC TCCCCGTCCG TGCGGTCCAA CATCCGGGTC
    ACGGTCACAG CCACCGCGGT CATCATCAAC CTGGTGGTCA TCATTCTCCT GGACGAGGTG
    TACGGCTGCA TAGCCAGATG GCTCACCAAG ATAGAGGTTC CAAAGACGGA GAAGAGCTTT
    GAGGAGAGGC TGATCTTCAA GGCTTTCCTG CTCAAGTTCG TCAACTCCTA CACACCCATC
    TTCTACGTGG CTTTCTTCAA AGGCCGGTTT GTCGGACGCC CGGGTGACTA CGTGTACATT
    TTCCGATCTT TCCGGATGGA GGAGTGCGCC CCGGGGGGCT GCCTGATGGA GCTCTGCATC
    CAGCTCAGCA TCATCATGCT GGGAAAGCAG CTGATCCAGA ACAACCTGTT TGAGATCGGC
    ATCCCGAAAA TGAAGAAGTT CATCCGCTAT CTGAGGCTGA AGCACCAGAG CCCCTCGGAC
    CACGACGAGC ACGTGAAGAA GAAGCAGCGG TACGAGGTGG ACTACACGCT GGAGCCCTTC
    GCGGGCCTCA CCCCGGAGTA CATGGAGATG ATCATCCAGT TCGGCTTCGT CACCCTGTTT
    GTCGCATCCT TCCCGCTGGC CCCACTCTTC GCGCTCCTGA ACAACATCAT TGAGATCCGC
    CTGGACGCCA AAAAGTTTGT CACCGAGCTC CGAAGGCCGG TGGCTGTCAG AGCCAAGGAC
    ATCGGAATCT GGTACAATAT CCTCAGAGGC GTCGGGAAGC TTGCCGTCAT CATCAACGCG
    TTTGTGATCT CCTTCACGTC TGACTTCATC CCTCGCCTGG TGTACCTCTA CATGTACAGT
    GAGAACGGGA CCATGCATGG CTTCGTCAAC CACACCCTCT CTTCCTTCAA CGTCAGCGAC
    TTCCAGAACG GCACGGCCCC CAACGACCCC CTGGATCTGG GCTACGAGGT GCAGATCTGC
    AGGTACAAAG ACTACCGGGA GCCGCCATGG TCGGAACACA AGTATGACAT CTCCAAGGAC
    TTCTGGGCCG TCCTGGCCGC CCGGCTGGCC TTTGTCATCG TCTTCCAGAA CCTGGTCATG
    TTCATGAGCG ACTTTGTGGA CTGGGTCATC CCCGACATCC CCAAGGACAT CAGCCAGCAG
    ATCCACAAAG AGAAGGTGCT CATGGTGGAG CTGTTCATGC GGGAGGAGCA GGGCAAGCAG
    CAGCTGCTGG ACACGTGGAT GGAGAAGGAC CGGAAGAAGG CGGAGCCCTG CAACAACCAC
    AGCCCCGCCC CAGCCGCCCC GCCCCTGCCC CCGCCCCCGG AAGGCCCCGA CTTCCCCGGA
    GGCTCCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232025
    Clone ID Related Accession (Same CDS sequence) XM_024987197.1
    Accession Version XM_024987197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GACCATCCCA GAGCTGAATA TGAAGCCCGA
    GTTCTGGAGA AGTCTCTTAG GAAAGAATCC AAAAACAAAG AGAAGCGCCG GCACCTTCCG
    GAGGAGCCCA CAAACAAGTG GAGGCAGAGG GTTAAGACCG CCATGGCGGG GGTGAAATTG
    ATCCACCCCA TTCTAGAAAC ATCCCAGACA GACAAGGTGA AGCTGACCTG GAGAGACCGG
    TTCCCTGCCT ACTTCACCAA TTTGGTGTCT ATCATCTTCA TGATCGCCGT GACCTTTGCC
    ATTGTCCTTG GCGTCATCAT CTACAGAATC TCCACGGCCG CCGCCTTGGC CATGAACTCC
    TCCCCGTCCG TGCGGTCCAA CATCCGGGTC ACGGTCACAG CCACCGCGGT CATCATCAAC
    CTGGTGGTCA TCATTCTCCT GGACGAGGTG TACGGCTGCA TAGCCAGATG GCTCACCAAG
    ATAGAGGTTC CAAAGACGGA GAAGAGCTTT GAGGAGAGGC TGATCTTCAA GGCTTTCCTG
    CTCAAGTTCG TCAACTCCTA CACACCCATC TTCTACGTGG CTTTCTTCAA AGGCCGGTTT
    GTCGGACGCC CGGGTGACTA CGTGTACATT TTCCGATCTT TCCGGATGGA GGAGTGCGCC
    CCGGGGGGCT GCCTGATGGA GCTCTGCATC CAGCTCAGCA TCATCATGCT GGGAAAGCAG
    CTGATCCAGA ACAACCTGTT TGAGATCGGC ATCCCGAAAA TGAAGAAGTT CATCCGCTAT
    CTGAGGCTGA AGCACCAGAG CCCCTCGGAC CACGACGAGC ACGTGAAGAA GAAGCAGCGG
    TACGAGGTGG ACTACACGCT GGAGCCCTTC GCGGGCCTCA CCCCGGAGTA CATGGAGATG
    ATCATCCAGT TCGGCTTCGT CACCCTGTTT GTCGCATCCT TCCCGCTGGC CCCACTCTTC
    GCGCTCCTGA ACAACATCAT TGAGATCCGC CTGGACGCCA AAAAGTTTGT CACCGAGCTC
    CGAAGGCCGG TGGCTGTCAG AGCCAAGGAC ATCGGAATCT GGTACAATAT CCTCAGAGGC
    GTCGGGAAGC TTGCCGTCAT CATCAACGCG TTTGTGATCT CCTTCACGTC TGACTTCATC
    CCTCGCCTGG TGTACCTCTA CATGTACAGT GAGAACGGGA CCATGCATGG CTTCGTCAAC
    CACACCCTCT CTTCCTTCAA CGTCAGCGAC TTCCAGAACG GCACGGCCCC CAACGACCCC
    CTGGATCTGG GCTACGAGGT GCAGATCTGC AGGTACAAAG ACTACCGGGA GCCGCCATGG
    TCGGAACACA AGTATGACAT CTCCAAGGAC TTCTGGGCCG TCCTGGCCGC CCGGCTGGCC
    TTTGTCATCG TCTTCCAGAA CCTGGTCATG TTCATGAGCG ACTTTGTGGA CTGGGTCATC
    CCCGACATCC CCAAGGACAT CAGCCAGCAG ATCCACAAAG AGAAGGTGCT CATGGTGGAG
    CTGTTCATGC GGGAGGAGCA GGGCAAGCAG CAGCTGCTGG ACACGTGGAT GGAGAAGGAC
    CGGAAGAAGG CGGAGCCCTG CAACAACCAC AGCCCCGCCC CAGCCGCCCC GCCCCTGCCC
    CCGCCCCCGG AAGGCCCCGA CTTCCCCGGA GGCTCCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842965.1
    CDS263..3424
    Translation

    Target ORF information:

    RefSeq Version XM_024987197.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GACCATCCCA GAGCTGAATA TGAAGCCCGA
    GTTCTGGAGA AGTCTCTTAG GAAAGAATCC AAAAACAAAG AGAAGCGCCG GCACCTTCCG
    GAGGAGCCCA CAAACAAGTG GAGGCAGAGG GTTAAGACCG CCATGGCGGG GGTGAAATTG
    ATCCACCCCA TTCTAGAAAC ATCCCAGACA GACAAGGTGA AGCTGACCTG GAGAGACCGG
    TTCCCTGCCT ACTTCACCAA TTTGGTGTCT ATCATCTTCA TGATCGCCGT GACCTTTGCC
    ATTGTCCTTG GCGTCATCAT CTACAGAATC TCCACGGCCG CCGCCTTGGC CATGAACTCC
    TCCCCGTCCG TGCGGTCCAA CATCCGGGTC ACGGTCACAG CCACCGCGGT CATCATCAAC
    CTGGTGGTCA TCATTCTCCT GGACGAGGTG TACGGCTGCA TAGCCAGATG GCTCACCAAG
    ATAGAGGTTC CAAAGACGGA GAAGAGCTTT GAGGAGAGGC TGATCTTCAA GGCTTTCCTG
    CTCAAGTTCG TCAACTCCTA CACACCCATC TTCTACGTGG CTTTCTTCAA AGGCCGGTTT
    GTCGGACGCC CGGGTGACTA CGTGTACATT TTCCGATCTT TCCGGATGGA GGAGTGCGCC
    CCGGGGGGCT GCCTGATGGA GCTCTGCATC CAGCTCAGCA TCATCATGCT GGGAAAGCAG
    CTGATCCAGA ACAACCTGTT TGAGATCGGC ATCCCGAAAA TGAAGAAGTT CATCCGCTAT
    CTGAGGCTGA AGCACCAGAG CCCCTCGGAC CACGACGAGC ACGTGAAGAA GAAGCAGCGG
    TACGAGGTGG ACTACACGCT GGAGCCCTTC GCGGGCCTCA CCCCGGAGTA CATGGAGATG
    ATCATCCAGT TCGGCTTCGT CACCCTGTTT GTCGCATCCT TCCCGCTGGC CCCACTCTTC
    GCGCTCCTGA ACAACATCAT TGAGATCCGC CTGGACGCCA AAAAGTTTGT CACCGAGCTC
    CGAAGGCCGG TGGCTGTCAG AGCCAAGGAC ATCGGAATCT GGTACAATAT CCTCAGAGGC
    GTCGGGAAGC TTGCCGTCAT CATCAACGCG TTTGTGATCT CCTTCACGTC TGACTTCATC
    CCTCGCCTGG TGTACCTCTA CATGTACAGT GAGAACGGGA CCATGCATGG CTTCGTCAAC
    CACACCCTCT CTTCCTTCAA CGTCAGCGAC TTCCAGAACG GCACGGCCCC CAACGACCCC
    CTGGATCTGG GCTACGAGGT GCAGATCTGC AGGTACAAAG ACTACCGGGA GCCGCCATGG
    TCGGAACACA AGTATGACAT CTCCAAGGAC TTCTGGGCCG TCCTGGCCGC CCGGCTGGCC
    TTTGTCATCG TCTTCCAGAA CCTGGTCATG TTCATGAGCG ACTTTGTGGA CTGGGTCATC
    CCCGACATCC CCAAGGACAT CAGCCAGCAG ATCCACAAAG AGAAGGTGCT CATGGTGGAG
    CTGTTCATGC GGGAGGAGCA GGGCAAGCAG CAGCTGCTGG ACACGTGGAT GGAGAAGGAC
    CGGAAGAAGG CGGAGCCCTG CAACAACCAC AGCCCCGCCC CAGCCGCCCC GCCCCTGCCC
    CCGCCCCCGG AAGGCCCCGA CTTCCCCGGA GGCTCCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232026
    Clone ID Related Accession (Same CDS sequence) XM_024987199.1
    Accession Version XM_024987199.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGACCAT CCCAGAGCTG AATATGAAGC CCGAGTTCTG
    GAGAAGTCTC TTAGGAAAGA ATCCAAAAAC AAAGAGAAGC GCCGGCACCT TCCGGAGGAG
    CCCACAAACA AGTGGAGGCA GAGGGTTAAG ACCGCCATGG CGGGGGTGAA ATTGATCCAC
    CCCATTCTAG AAACATCCCA GACAGACAAG GTGAAGCTGA CCTGGAGAGA CCGGTTCCCT
    GCCTACTTCA CCAATTTGGT GTCTATCATC TTCATGATCG CCGTGACCTT TGCCATTGTC
    CTTGGCGTCA TCATCTACAG AATCTCCACG GCCGCCGCCT TGGCCATGAA CTCCTCCCCG
    TCCGTGCGGT CCAACATCCG GGTCACGGTC ACAGCCACCG CGGTCATCAT CAACCTGGTG
    GTCATCATTC TCCTGGACGA GGTGTACGGC TGCATAGCCA GATGGCTCAC CAAGATAGAG
    GTTCCAAAGA CGGAGAAGAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT CCTGCTCAAG
    TTCGTCAACT CCTACACACC CATCTTCTAC GTGGCTTTCT TCAAAGGCCG GTTTGTCGGA
    CGCCCGGGTG ACTACGTGTA CATTTTCCGA TCTTTCCGGA TGGAGGAGTG CGCCCCGGGG
    GGCTGCCTGA TGGAGCTCTG CATCCAGCTC AGCATCATCA TGCTGGGAAA GCAGCTGATC
    CAGAACAACC TGTTTGAGAT CGGCATCCCG AAAATGAAGA AGTTCATCCG CTATCTGAGG
    CTGAAGCACC AGAGCCCCTC GGACCACGAC GAGCACGTGA AGAAGAAGCA GCGGTACGAG
    GTGGACTACA CGCTGGAGCC CTTCGCGGGC CTCACCCCGG AGTACATGGA GATGATCATC
    CAGTTCGGCT TCGTCACCCT GTTTGTCGCA TCCTTCCCGC TGGCCCCACT CTTCGCGCTC
    CTGAACAACA TCATTGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACCGA GCTCCGAAGG
    CCGGTGGCTG TCAGAGCCAA GGACATCGGA ATCTGGTACA ATATCCTCAG AGGCGTCGGG
    AAGCTTGCCG TCATCATCAA CGCGTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC
    CTGGTGTACC TCTACATGTA CAGTGAGAAC GGGACCATGC ATGGCTTCGT CAACCACACC
    CTCTCTTCCT TCAACGTCAG CGACTTCCAG AACGGCACGG CCCCCAACGA CCCCCTGGAT
    CTGGGCTACG AGGTGCAGAT CTGCAGGTAC AAAGACTACC GGGAGCCGCC ATGGTCGGAA
    CACAAGTATG ACATCTCCAA GGACTTCTGG GCCGTCCTGG CCGCCCGGCT GGCCTTTGTC
    ATCGTCTTCC AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT CATCCCCGAC
    ATCCCCAAGG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TGCTCATGGT GGAGCTGTTC
    ATGCGGGAGG AGCAGGGCAA GCAGCAGCTG CTGGACACGT GGATGGAGAA GGACCGGAAG
    AAGGCGGAGC CCTGCAACAA CCACAGCCCC GCCCCAGCCG CCCCGCCCCT GCCCCCGCCC
    CCGGAAGGCC CCGACTTCCC CGGAGGCTCC CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842967.1
    CDS262..3357
    Translation

    Target ORF information:

    RefSeq Version XM_024987199.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987199.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGACCAT CCCAGAGCTG AATATGAAGC CCGAGTTCTG
    GAGAAGTCTC TTAGGAAAGA ATCCAAAAAC AAAGAGAAGC GCCGGCACCT TCCGGAGGAG
    CCCACAAACA AGTGGAGGCA GAGGGTTAAG ACCGCCATGG CGGGGGTGAA ATTGATCCAC
    CCCATTCTAG AAACATCCCA GACAGACAAG GTGAAGCTGA CCTGGAGAGA CCGGTTCCCT
    GCCTACTTCA CCAATTTGGT GTCTATCATC TTCATGATCG CCGTGACCTT TGCCATTGTC
    CTTGGCGTCA TCATCTACAG AATCTCCACG GCCGCCGCCT TGGCCATGAA CTCCTCCCCG
    TCCGTGCGGT CCAACATCCG GGTCACGGTC ACAGCCACCG CGGTCATCAT CAACCTGGTG
    GTCATCATTC TCCTGGACGA GGTGTACGGC TGCATAGCCA GATGGCTCAC CAAGATAGAG
    GTTCCAAAGA CGGAGAAGAG CTTTGAGGAG AGGCTGATCT TCAAGGCTTT CCTGCTCAAG
    TTCGTCAACT CCTACACACC CATCTTCTAC GTGGCTTTCT TCAAAGGCCG GTTTGTCGGA
    CGCCCGGGTG ACTACGTGTA CATTTTCCGA TCTTTCCGGA TGGAGGAGTG CGCCCCGGGG
    GGCTGCCTGA TGGAGCTCTG CATCCAGCTC AGCATCATCA TGCTGGGAAA GCAGCTGATC
    CAGAACAACC TGTTTGAGAT CGGCATCCCG AAAATGAAGA AGTTCATCCG CTATCTGAGG
    CTGAAGCACC AGAGCCCCTC GGACCACGAC GAGCACGTGA AGAAGAAGCA GCGGTACGAG
    GTGGACTACA CGCTGGAGCC CTTCGCGGGC CTCACCCCGG AGTACATGGA GATGATCATC
    CAGTTCGGCT TCGTCACCCT GTTTGTCGCA TCCTTCCCGC TGGCCCCACT CTTCGCGCTC
    CTGAACAACA TCATTGAGAT CCGCCTGGAC GCCAAAAAGT TTGTCACCGA GCTCCGAAGG
    CCGGTGGCTG TCAGAGCCAA GGACATCGGA ATCTGGTACA ATATCCTCAG AGGCGTCGGG
    AAGCTTGCCG TCATCATCAA CGCGTTTGTG ATCTCCTTCA CGTCTGACTT CATCCCTCGC
    CTGGTGTACC TCTACATGTA CAGTGAGAAC GGGACCATGC ATGGCTTCGT CAACCACACC
    CTCTCTTCCT TCAACGTCAG CGACTTCCAG AACGGCACGG CCCCCAACGA CCCCCTGGAT
    CTGGGCTACG AGGTGCAGAT CTGCAGGTAC AAAGACTACC GGGAGCCGCC ATGGTCGGAA
    CACAAGTATG ACATCTCCAA GGACTTCTGG GCCGTCCTGG CCGCCCGGCT GGCCTTTGTC
    ATCGTCTTCC AGAACCTGGT CATGTTCATG AGCGACTTTG TGGACTGGGT CATCCCCGAC
    ATCCCCAAGG ACATCAGCCA GCAGATCCAC AAAGAGAAGG TGCTCATGGT GGAGCTGTTC
    ATGCGGGAGG AGCAGGGCAA GCAGCAGCTG CTGGACACGT GGATGGAGAA GGACCGGAAG
    AAGGCGGAGC CCTGCAACAA CCACAGCCCC GCCCCAGCCG CCCCGCCCCT GCCCCCGCCC
    CCGGAAGGCC CCGACTTCCC CGGAGGCTCC CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39758
    Clone ID Related Accession (Same CDS sequence) NM_001192717.1
    Accession Version NM_001192717.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-13
    Organism Bos taurus(cattle)
    Product anoctamin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000029.1. On Jul 17, 2010 this sequence version replaced XM_610636.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTCTA CTCGGCGGCC
    TACCCGCTGC ATGATGGAGA CTATGAAGGA GACAACGTGG AGTTCAATGA CAGGAAGCTC
    CTGTACGAAG AGTGGGCGAG TTACGGGGTC TTCTACAAGT ACCAGCCCAT CGACCTCGTC
    AGAAAGTATT TTGGGGAGAA GATTGGTCTG TACTTCGCCT GGCTTGGCGT CTACACCCAG
    ATGCTCATCC CGGCCTCCGT GGTCGGCATC ATCGTTTTCC TCTACGGATG GGCCACTGTC
    GATGACAACA TCCCCAGCAT GGAGATGTGC GACCAGAGGC ACAACATCAC CATGTGCCCG
    CTCTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCCG CGTGCGCCAC CGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACTGTC TTCTTCTCCA TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGGC TCAATTACCG GTGGGACCTC
    ACAGGCTTCG AGGAGGAGGA GGAGGCTGTC AAGGACCATC CCAGAGCTGA ATATGAAGCC
    CGAGTTCTGG AGAAGTCTCT TAGGAAAGAA TCCAAAAACA AAGAGAAGCG CCGGCACCTT
    CCGGAGGAGC CCACAAACAA GTGGAGGCAG AGGGTTAAGA CCGCCATGGC GGGGGTGAAA
    TTGACAGACA AGGTGAAGCT GACCTGGAGA GACCGGTTCC CTGCCTACTT CACCAATTTG
    GTGTCTATCA TCTTCATGAT CGCCGTGACC TTTGCCATTG TCCTTGGCGT CATCATCTAC
    AGAATCTCCA CGGCCGCCGC CTTGGCCATG AACTCCTCCC CGTCCGTGCG GTCCAACATC
    CGGGTCACGG TCACAGCCAC CGCGGTCATC ATCAACCTGG TGGTCATCAT TCTCCTGGAC
    GAGGTGTACG GCTGCATAGC CAGATGGCTC ACCAAGATAG AGGTTCCAAA GACGGAGAAG
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTCA AGTTCGTCAA CTCCTACACA
    CCCATCTTCT ACGTGGCTTT CTTCAAAGGC CGGTTTGTCG GACGCCCGGG TGACTACGTG
    TACATTTTCC GATCTTTCCG GATGGAGGAG TGCGCCCCGG GGGGCTGCCT GATGGAGCTC
    TGCATCCAGC TCAGCATCAT CATGCTGGGA AAGCAGCTGA TCCAGAACAA CCTGTTTGAG
    ATCGGCATCC CGAAAATGAA GAAGTTCATC CGCTATCTGA GGCTGAAGCA CCAGAGCCCC
    TCGGACCACG ACGAGCACGT GAAGAAGAAG CAGCGGTACG AGGTGGACTA CACGCTGGAG
    CCCTTCGCGG GCCTCACCCC GGAGTACATG GAGATGATCA TCCAGTTCGG CTTCGTCACC
    CTGTTTGTCG CATCCTTCCC GCTGGCCCCA CTCTTCGCGC TCCTGAACAA CATCATTGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACC GAGCTCCGAA GGCCGGTGGC TGTCAGAGCC
    AAGGACATCG GAATCTGGTA CAATATCCTC AGAGGCGTCG GGAAGCTTGC CGTCATCATC
    AACGCGTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG
    TACAGTGAGA ACGGGACCAT GCATGGCTTC GTCAACCACA CCCTCTCTTC CTTCAACGTC
    AGCGACTTCC AGAACGGCAC GGCCCCCAAC GACCCCCTGG ATCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ACAAAGACTA CCGGGAGCCG CCATGGTCGG AACACAAGTA TGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCCGCCCGG CTGGCCTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCCG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAAGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAGGGC
    AAGCAGCAGC TGCTGGACAC GTGGATGGAG AAGGACCGGA AGAAGGCGGA GCCCTGCAAC
    AACCACAGCC CCGCCCCAGC CGCCCCGCCC CTGCCCCCGC CCCCGGAAGG CCCCGACTTC
    CCCGGAGGCT CCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179646.1
    CDS1..2958
    Translation

    Target ORF information:

    RefSeq Version NM_001192717.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTCTA CTCGGCGGCC
    TACCCGCTGC ATGATGGAGA CTATGAAGGA GACAACGTGG AGTTCAATGA CAGGAAGCTC
    CTGTACGAAG AGTGGGCGAG TTACGGGGTC TTCTACAAGT ACCAGCCCAT CGACCTCGTC
    AGAAAGTATT TTGGGGAGAA GATTGGTCTG TACTTCGCCT GGCTTGGCGT CTACACCCAG
    ATGCTCATCC CGGCCTCCGT GGTCGGCATC ATCGTTTTCC TCTACGGATG GGCCACTGTC
    GATGACAACA TCCCCAGCAT GGAGATGTGC GACCAGAGGC ACAACATCAC CATGTGCCCG
    CTCTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCCG CGTGCGCCAC CGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACTGTC TTCTTCTCCA TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGGC TCAATTACCG GTGGGACCTC
    ACAGGCTTCG AGGAGGAGGA GGAGGCTGTC AAGGACCATC CCAGAGCTGA ATATGAAGCC
    CGAGTTCTGG AGAAGTCTCT TAGGAAAGAA TCCAAAAACA AAGAGAAGCG CCGGCACCTT
    CCGGAGGAGC CCACAAACAA GTGGAGGCAG AGGGTTAAGA CCGCCATGGC GGGGGTGAAA
    TTGACAGACA AGGTGAAGCT GACCTGGAGA GACCGGTTCC CTGCCTACTT CACCAATTTG
    GTGTCTATCA TCTTCATGAT CGCCGTGACC TTTGCCATTG TCCTTGGCGT CATCATCTAC
    AGAATCTCCA CGGCCGCCGC CTTGGCCATG AACTCCTCCC CGTCCGTGCG GTCCAACATC
    CGGGTCACGG TCACAGCCAC CGCGGTCATC ATCAACCTGG TGGTCATCAT TCTCCTGGAC
    GAGGTGTACG GCTGCATAGC CAGATGGCTC ACCAAGATAG AGGTTCCAAA GACGGAGAAG
    AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT TTCCTGCTCA AGTTCGTCAA CTCCTACACA
    CCCATCTTCT ACGTGGCTTT CTTCAAAGGC CGGTTTGTCG GACGCCCGGG TGACTACGTG
    TACATTTTCC GATCTTTCCG GATGGAGGAG TGCGCCCCGG GGGGCTGCCT GATGGAGCTC
    TGCATCCAGC TCAGCATCAT CATGCTGGGA AAGCAGCTGA TCCAGAACAA CCTGTTTGAG
    ATCGGCATCC CGAAAATGAA GAAGTTCATC CGCTATCTGA GGCTGAAGCA CCAGAGCCCC
    TCGGACCACG ACGAGCACGT GAAGAAGAAG CAGCGGTACG AGGTGGACTA CACGCTGGAG
    CCCTTCGCGG GCCTCACCCC GGAGTACATG GAGATGATCA TCCAGTTCGG CTTCGTCACC
    CTGTTTGTCG CATCCTTCCC GCTGGCCCCA CTCTTCGCGC TCCTGAACAA CATCATTGAG
    ATCCGCCTGG ACGCCAAAAA GTTTGTCACC GAGCTCCGAA GGCCGGTGGC TGTCAGAGCC
    AAGGACATCG GAATCTGGTA CAATATCCTC AGAGGCGTCG GGAAGCTTGC CGTCATCATC
    AACGCGTTTG TGATCTCCTT CACGTCTGAC TTCATCCCTC GCCTGGTGTA CCTCTACATG
    TACAGTGAGA ACGGGACCAT GCATGGCTTC GTCAACCACA CCCTCTCTTC CTTCAACGTC
    AGCGACTTCC AGAACGGCAC GGCCCCCAAC GACCCCCTGG ATCTGGGCTA CGAGGTGCAG
    ATCTGCAGGT ACAAAGACTA CCGGGAGCCG CCATGGTCGG AACACAAGTA TGACATCTCC
    AAGGACTTCT GGGCCGTCCT GGCCGCCCGG CTGGCCTTTG TCATCGTCTT CCAGAACCTG
    GTCATGTTCA TGAGCGACTT TGTGGACTGG GTCATCCCCG ACATCCCCAA GGACATCAGC
    CAGCAGATCC ACAAAGAGAA GGTGCTCATG GTGGAGCTGT TCATGCGGGA GGAGCAGGGC
    AAGCAGCAGC TGCTGGACAC GTGGATGGAG AAGGACCGGA AGAAGGCGGA GCCCTGCAAC
    AACCACAGCC CCGCCCCAGC CGCCCCGCCC CTGCCCCCGC CCCCGGAAGG CCCCGACTTC
    CCCGGAGGCT CCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52105
    Clone ID Related Accession (Same CDS sequence) XM_024987196.1
    Accession Version XM_024987196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGAAGCGC
    CGGCACCTTC CGGAGGAGCC CACAAACAAG TGGAGGCAGA GGGTTAAGAC CGCCATGGCG
    GGGGTGAAAT TGATCCACCC CATTCTAGAA ACATCCCAGA CAGACAAGGT GAAGCTGACC
    TGGAGAGACC GGTTCCCTGC CTACTTCACC AATTTGGTGT CTATCATCTT CATGATCGCC
    GTGACCTTTG CCATTGTCCT TGGCGTCATC ATCTACAGAA TCTCCACGGC CGCCGCCTTG
    GCCATGAACT CCTCCCCGTC CGTGCGGTCC AACATCCGGG TCACGGTCAC AGCCACCGCG
    GTCATCATCA ACCTGGTGGT CATCATTCTC CTGGACGAGG TGTACGGCTG CATAGCCAGA
    TGGCTCACCA AGATAGAGGT TCCAAAGACG GAGAAGAGCT TTGAGGAGAG GCTGATCTTC
    AAGGCTTTCC TGCTCAAGTT CGTCAACTCC TACACACCCA TCTTCTACGT GGCTTTCTTC
    AAAGGCCGGT TTGTCGGACG CCCGGGTGAC TACGTGTACA TTTTCCGATC TTTCCGGATG
    GAGGAGTGCG CCCCGGGGGG CTGCCTGATG GAGCTCTGCA TCCAGCTCAG CATCATCATG
    CTGGGAAAGC AGCTGATCCA GAACAACCTG TTTGAGATCG GCATCCCGAA AATGAAGAAG
    TTCATCCGCT ATCTGAGGCT GAAGCACCAG AGCCCCTCGG ACCACGACGA GCACGTGAAG
    AAGAAGCAGC GGTACGAGGT GGACTACACG CTGGAGCCCT TCGCGGGCCT CACCCCGGAG
    TACATGGAGA TGATCATCCA GTTCGGCTTC GTCACCCTGT TTGTCGCATC CTTCCCGCTG
    GCCCCACTCT TCGCGCTCCT GAACAACATC ATTGAGATCC GCCTGGACGC CAAAAAGTTT
    GTCACCGAGC TCCGAAGGCC GGTGGCTGTC AGAGCCAAGG ACATCGGAAT CTGGTACAAT
    ATCCTCAGAG GCGTCGGGAA GCTTGCCGTC ATCATCAACG CGTTTGTGAT CTCCTTCACG
    TCTGACTTCA TCCCTCGCCT GGTGTACCTC TACATGTACA GTGAGAACGG GACCATGCAT
    GGCTTCGTCA ACCACACCCT CTCTTCCTTC AACGTCAGCG ACTTCCAGAA CGGCACGGCC
    CCCAACGACC CCCTGGATCT GGGCTACGAG GTGCAGATCT GCAGGTACAA AGACTACCGG
    GAGCCGCCAT GGTCGGAACA CAAGTATGAC ATCTCCAAGG ACTTCTGGGC CGTCCTGGCC
    GCCCGGCTGG CCTTTGTCAT CGTCTTCCAG AACCTGGTCA TGTTCATGAG CGACTTTGTG
    GACTGGGTCA TCCCCGACAT CCCCAAGGAC ATCAGCCAGC AGATCCACAA AGAGAAGGTG
    CTCATGGTGG AGCTGTTCAT GCGGGAGGAG CAGGGCAAGC AGCAGCTGCT GGACACGTGG
    ATGGAGAAGG ACCGGAAGAA GGCGGAGCCC TGCAACAACC ACAGCCCCGC CCCAGCCGCC
    CCGCCCCTGC CCCCGCCCCC GGAAGGCCCC GACTTCCCCG GAGGCTCCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842964.1
    CDS262..3435
    Translation

    Target ORF information:

    RefSeq Version XM_024987196.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGAAGCGC
    CGGCACCTTC CGGAGGAGCC CACAAACAAG TGGAGGCAGA GGGTTAAGAC CGCCATGGCG
    GGGGTGAAAT TGATCCACCC CATTCTAGAA ACATCCCAGA CAGACAAGGT GAAGCTGACC
    TGGAGAGACC GGTTCCCTGC CTACTTCACC AATTTGGTGT CTATCATCTT CATGATCGCC
    GTGACCTTTG CCATTGTCCT TGGCGTCATC ATCTACAGAA TCTCCACGGC CGCCGCCTTG
    GCCATGAACT CCTCCCCGTC CGTGCGGTCC AACATCCGGG TCACGGTCAC AGCCACCGCG
    GTCATCATCA ACCTGGTGGT CATCATTCTC CTGGACGAGG TGTACGGCTG CATAGCCAGA
    TGGCTCACCA AGATAGAGGT TCCAAAGACG GAGAAGAGCT TTGAGGAGAG GCTGATCTTC
    AAGGCTTTCC TGCTCAAGTT CGTCAACTCC TACACACCCA TCTTCTACGT GGCTTTCTTC
    AAAGGCCGGT TTGTCGGACG CCCGGGTGAC TACGTGTACA TTTTCCGATC TTTCCGGATG
    GAGGAGTGCG CCCCGGGGGG CTGCCTGATG GAGCTCTGCA TCCAGCTCAG CATCATCATG
    CTGGGAAAGC AGCTGATCCA GAACAACCTG TTTGAGATCG GCATCCCGAA AATGAAGAAG
    TTCATCCGCT ATCTGAGGCT GAAGCACCAG AGCCCCTCGG ACCACGACGA GCACGTGAAG
    AAGAAGCAGC GGTACGAGGT GGACTACACG CTGGAGCCCT TCGCGGGCCT CACCCCGGAG
    TACATGGAGA TGATCATCCA GTTCGGCTTC GTCACCCTGT TTGTCGCATC CTTCCCGCTG
    GCCCCACTCT TCGCGCTCCT GAACAACATC ATTGAGATCC GCCTGGACGC CAAAAAGTTT
    GTCACCGAGC TCCGAAGGCC GGTGGCTGTC AGAGCCAAGG ACATCGGAAT CTGGTACAAT
    ATCCTCAGAG GCGTCGGGAA GCTTGCCGTC ATCATCAACG CGTTTGTGAT CTCCTTCACG
    TCTGACTTCA TCCCTCGCCT GGTGTACCTC TACATGTACA GTGAGAACGG GACCATGCAT
    GGCTTCGTCA ACCACACCCT CTCTTCCTTC AACGTCAGCG ACTTCCAGAA CGGCACGGCC
    CCCAACGACC CCCTGGATCT GGGCTACGAG GTGCAGATCT GCAGGTACAA AGACTACCGG
    GAGCCGCCAT GGTCGGAACA CAAGTATGAC ATCTCCAAGG ACTTCTGGGC CGTCCTGGCC
    GCCCGGCTGG CCTTTGTCAT CGTCTTCCAG AACCTGGTCA TGTTCATGAG CGACTTTGTG
    GACTGGGTCA TCCCCGACAT CCCCAAGGAC ATCAGCCAGC AGATCCACAA AGAGAAGGTG
    CTCATGGTGG AGCTGTTCAT GCGGGAGGAG CAGGGCAAGC AGCAGCTGCT GGACACGTGG
    ATGGAGAAGG ACCGGAAGAA GGCGGAGCCC TGCAACAACC ACAGCCCCGC CCCAGCCGCC
    CCGCCCCTGC CCCCGCCCCC GGAAGGCCCC GACTTCCCCG GAGGCTCCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52107
    Clone ID Related Accession (Same CDS sequence) XM_015461360.2
    Accession Version XM_015461360.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGAAGCGC
    CGGCACCTTC CGGAGGAGCC CACAAACAAG TGGAGGCAGA GGGTTAAGAC CGCCATGGCG
    GGGGTGAAAT TGACAGACAA GGTGAAGCTG ACCTGGAGAG ACCGGTTCCC TGCCTACTTC
    ACCAATTTGG TGTCTATCAT CTTCATGATC GCCGTGACCT TTGCCATTGT CCTTGGCGTC
    ATCATCTACA GAATCTCCAC GGCCGCCGCC TTGGCCATGA ACTCCTCCCC GTCCGTGCGG
    TCCAACATCC GGGTCACGGT CACAGCCACC GCGGTCATCA TCAACCTGGT GGTCATCATT
    CTCCTGGACG AGGTGTACGG CTGCATAGCC AGATGGCTCA CCAAGATAGA GGTTCCAAAG
    ACGGAGAAGA GCTTTGAGGA GAGGCTGATC TTCAAGGCTT TCCTGCTCAA GTTCGTCAAC
    TCCTACACAC CCATCTTCTA CGTGGCTTTC TTCAAAGGCC GGTTTGTCGG ACGCCCGGGT
    GACTACGTGT ACATTTTCCG ATCTTTCCGG ATGGAGGAGT GCGCCCCGGG GGGCTGCCTG
    ATGGAGCTCT GCATCCAGCT CAGCATCATC ATGCTGGGAA AGCAGCTGAT CCAGAACAAC
    CTGTTTGAGA TCGGCATCCC GAAAATGAAG AAGTTCATCC GCTATCTGAG GCTGAAGCAC
    CAGAGCCCCT CGGACCACGA CGAGCACGTG AAGAAGAAGC AGCGGTACGA GGTGGACTAC
    ACGCTGGAGC CCTTCGCGGG CCTCACCCCG GAGTACATGG AGATGATCAT CCAGTTCGGC
    TTCGTCACCC TGTTTGTCGC ATCCTTCCCG CTGGCCCCAC TCTTCGCGCT CCTGAACAAC
    ATCATTGAGA TCCGCCTGGA CGCCAAAAAG TTTGTCACCG AGCTCCGAAG GCCGGTGGCT
    GTCAGAGCCA AGGACATCGG AATCTGGTAC AATATCCTCA GAGGCGTCGG GAAGCTTGCC
    GTCATCATCA ACGCGTTTGT GATCTCCTTC ACGTCTGACT TCATCCCTCG CCTGGTGTAC
    CTCTACATGT ACAGTGAGAA CGGGACCATG CATGGCTTCG TCAACCACAC CCTCTCTTCC
    TTCAACGTCA GCGACTTCCA GAACGGCACG GCCCCCAACG ACCCCCTGGA TCTGGGCTAC
    GAGGTGCAGA TCTGCAGGTA CAAAGACTAC CGGGAGCCGC CATGGTCGGA ACACAAGTAT
    GACATCTCCA AGGACTTCTG GGCCGTCCTG GCCGCCCGGC TGGCCTTTGT CATCGTCTTC
    CAGAACCTGG TCATGTTCAT GAGCGACTTT GTGGACTGGG TCATCCCCGA CATCCCCAAG
    GACATCAGCC AGCAGATCCA CAAAGAGAAG GTGCTCATGG TGGAGCTGTT CATGCGGGAG
    GAGCAGGGCA AGCAGCAGCT GCTGGACACG TGGATGGAGA AGGACCGGAA GAAGGCGGAG
    CCCTGCAACA ACCACAGCCC CGCCCCAGCC GCCCCGCCCC TGCCCCCGCC CCCGGAAGGC
    CCCGACTTCC CCGGAGGCTC CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316846.2
    CDS261..3407
    Translation

    Target ORF information:

    RefSeq Version XM_015461360.2
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGAAGCGC
    CGGCACCTTC CGGAGGAGCC CACAAACAAG TGGAGGCAGA GGGTTAAGAC CGCCATGGCG
    GGGGTGAAAT TGACAGACAA GGTGAAGCTG ACCTGGAGAG ACCGGTTCCC TGCCTACTTC
    ACCAATTTGG TGTCTATCAT CTTCATGATC GCCGTGACCT TTGCCATTGT CCTTGGCGTC
    ATCATCTACA GAATCTCCAC GGCCGCCGCC TTGGCCATGA ACTCCTCCCC GTCCGTGCGG
    TCCAACATCC GGGTCACGGT CACAGCCACC GCGGTCATCA TCAACCTGGT GGTCATCATT
    CTCCTGGACG AGGTGTACGG CTGCATAGCC AGATGGCTCA CCAAGATAGA GGTTCCAAAG
    ACGGAGAAGA GCTTTGAGGA GAGGCTGATC TTCAAGGCTT TCCTGCTCAA GTTCGTCAAC
    TCCTACACAC CCATCTTCTA CGTGGCTTTC TTCAAAGGCC GGTTTGTCGG ACGCCCGGGT
    GACTACGTGT ACATTTTCCG ATCTTTCCGG ATGGAGGAGT GCGCCCCGGG GGGCTGCCTG
    ATGGAGCTCT GCATCCAGCT CAGCATCATC ATGCTGGGAA AGCAGCTGAT CCAGAACAAC
    CTGTTTGAGA TCGGCATCCC GAAAATGAAG AAGTTCATCC GCTATCTGAG GCTGAAGCAC
    CAGAGCCCCT CGGACCACGA CGAGCACGTG AAGAAGAAGC AGCGGTACGA GGTGGACTAC
    ACGCTGGAGC CCTTCGCGGG CCTCACCCCG GAGTACATGG AGATGATCAT CCAGTTCGGC
    TTCGTCACCC TGTTTGTCGC ATCCTTCCCG CTGGCCCCAC TCTTCGCGCT CCTGAACAAC
    ATCATTGAGA TCCGCCTGGA CGCCAAAAAG TTTGTCACCG AGCTCCGAAG GCCGGTGGCT
    GTCAGAGCCA AGGACATCGG AATCTGGTAC AATATCCTCA GAGGCGTCGG GAAGCTTGCC
    GTCATCATCA ACGCGTTTGT GATCTCCTTC ACGTCTGACT TCATCCCTCG CCTGGTGTAC
    CTCTACATGT ACAGTGAGAA CGGGACCATG CATGGCTTCG TCAACCACAC CCTCTCTTCC
    TTCAACGTCA GCGACTTCCA GAACGGCACG GCCCCCAACG ACCCCCTGGA TCTGGGCTAC
    GAGGTGCAGA TCTGCAGGTA CAAAGACTAC CGGGAGCCGC CATGGTCGGA ACACAAGTAT
    GACATCTCCA AGGACTTCTG GGCCGTCCTG GCCGCCCGGC TGGCCTTTGT CATCGTCTTC
    CAGAACCTGG TCATGTTCAT GAGCGACTTT GTGGACTGGG TCATCCCCGA CATCCCCAAG
    GACATCAGCC AGCAGATCCA CAAAGAGAAG GTGCTCATGG TGGAGCTGTT CATGCGGGAG
    GAGCAGGGCA AGCAGCAGCT GCTGGACACG TGGATGGAGA AGGACCGGAA GAAGGCGGAG
    CCCTGCAACA ACCACAGCCC CGCCCCAGCC GCCCCGCCCC TGCCCCCGCC CCCGGAAGGC
    CCCGACTTCC CCGGAGGCTC CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52109
    Clone ID Related Accession (Same CDS sequence) XM_024987198.1
    Accession Version XM_024987198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAA GCGCCGGCAC
    CTTCCGGAGG AGCCCACAAA CAAGTGGAGG CAGAGGGTTA AGACCGCCAT GGCGGGGGTG
    AAATTGATCC ACCCCATTCT AGAAACATCC CAGACAGACA AGGTGAAGCT GACCTGGAGA
    GACCGGTTCC CTGCCTACTT CACCAATTTG GTGTCTATCA TCTTCATGAT CGCCGTGACC
    TTTGCCATTG TCCTTGGCGT CATCATCTAC AGAATCTCCA CGGCCGCCGC CTTGGCCATG
    AACTCCTCCC CGTCCGTGCG GTCCAACATC CGGGTCACGG TCACAGCCAC CGCGGTCATC
    ATCAACCTGG TGGTCATCAT TCTCCTGGAC GAGGTGTACG GCTGCATAGC CAGATGGCTC
    ACCAAGATAG AGGTTCCAAA GACGGAGAAG AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT
    TTCCTGCTCA AGTTCGTCAA CTCCTACACA CCCATCTTCT ACGTGGCTTT CTTCAAAGGC
    CGGTTTGTCG GACGCCCGGG TGACTACGTG TACATTTTCC GATCTTTCCG GATGGAGGAG
    TGCGCCCCGG GGGGCTGCCT GATGGAGCTC TGCATCCAGC TCAGCATCAT CATGCTGGGA
    AAGCAGCTGA TCCAGAACAA CCTGTTTGAG ATCGGCATCC CGAAAATGAA GAAGTTCATC
    CGCTATCTGA GGCTGAAGCA CCAGAGCCCC TCGGACCACG ACGAGCACGT GAAGAAGAAG
    CAGCGGTACG AGGTGGACTA CACGCTGGAG CCCTTCGCGG GCCTCACCCC GGAGTACATG
    GAGATGATCA TCCAGTTCGG CTTCGTCACC CTGTTTGTCG CATCCTTCCC GCTGGCCCCA
    CTCTTCGCGC TCCTGAACAA CATCATTGAG ATCCGCCTGG ACGCCAAAAA GTTTGTCACC
    GAGCTCCGAA GGCCGGTGGC TGTCAGAGCC AAGGACATCG GAATCTGGTA CAATATCCTC
    AGAGGCGTCG GGAAGCTTGC CGTCATCATC AACGCGTTTG TGATCTCCTT CACGTCTGAC
    TTCATCCCTC GCCTGGTGTA CCTCTACATG TACAGTGAGA ACGGGACCAT GCATGGCTTC
    GTCAACCACA CCCTCTCTTC CTTCAACGTC AGCGACTTCC AGAACGGCAC GGCCCCCAAC
    GACCCCCTGG ATCTGGGCTA CGAGGTGCAG ATCTGCAGGT ACAAAGACTA CCGGGAGCCG
    CCATGGTCGG AACACAAGTA TGACATCTCC AAGGACTTCT GGGCCGTCCT GGCCGCCCGG
    CTGGCCTTTG TCATCGTCTT CCAGAACCTG GTCATGTTCA TGAGCGACTT TGTGGACTGG
    GTCATCCCCG ACATCCCCAA GGACATCAGC CAGCAGATCC ACAAAGAGAA GGTGCTCATG
    GTGGAGCTGT TCATGCGGGA GGAGCAGGGC AAGCAGCAGC TGCTGGACAC GTGGATGGAG
    AAGGACCGGA AGAAGGCGGA GCCCTGCAAC AACCACAGCC CCGCCCCAGC CGCCCCGCCC
    CTGCCCCCGC CCCCGGAAGG CCCCGACTTC CCCGGAGGCT CCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842966.1
    CDS261..3368
    Translation

    Target ORF information:

    RefSeq Version XM_024987198.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAA GCGCCGGCAC
    CTTCCGGAGG AGCCCACAAA CAAGTGGAGG CAGAGGGTTA AGACCGCCAT GGCGGGGGTG
    AAATTGATCC ACCCCATTCT AGAAACATCC CAGACAGACA AGGTGAAGCT GACCTGGAGA
    GACCGGTTCC CTGCCTACTT CACCAATTTG GTGTCTATCA TCTTCATGAT CGCCGTGACC
    TTTGCCATTG TCCTTGGCGT CATCATCTAC AGAATCTCCA CGGCCGCCGC CTTGGCCATG
    AACTCCTCCC CGTCCGTGCG GTCCAACATC CGGGTCACGG TCACAGCCAC CGCGGTCATC
    ATCAACCTGG TGGTCATCAT TCTCCTGGAC GAGGTGTACG GCTGCATAGC CAGATGGCTC
    ACCAAGATAG AGGTTCCAAA GACGGAGAAG AGCTTTGAGG AGAGGCTGAT CTTCAAGGCT
    TTCCTGCTCA AGTTCGTCAA CTCCTACACA CCCATCTTCT ACGTGGCTTT CTTCAAAGGC
    CGGTTTGTCG GACGCCCGGG TGACTACGTG TACATTTTCC GATCTTTCCG GATGGAGGAG
    TGCGCCCCGG GGGGCTGCCT GATGGAGCTC TGCATCCAGC TCAGCATCAT CATGCTGGGA
    AAGCAGCTGA TCCAGAACAA CCTGTTTGAG ATCGGCATCC CGAAAATGAA GAAGTTCATC
    CGCTATCTGA GGCTGAAGCA CCAGAGCCCC TCGGACCACG ACGAGCACGT GAAGAAGAAG
    CAGCGGTACG AGGTGGACTA CACGCTGGAG CCCTTCGCGG GCCTCACCCC GGAGTACATG
    GAGATGATCA TCCAGTTCGG CTTCGTCACC CTGTTTGTCG CATCCTTCCC GCTGGCCCCA
    CTCTTCGCGC TCCTGAACAA CATCATTGAG ATCCGCCTGG ACGCCAAAAA GTTTGTCACC
    GAGCTCCGAA GGCCGGTGGC TGTCAGAGCC AAGGACATCG GAATCTGGTA CAATATCCTC
    AGAGGCGTCG GGAAGCTTGC CGTCATCATC AACGCGTTTG TGATCTCCTT CACGTCTGAC
    TTCATCCCTC GCCTGGTGTA CCTCTACATG TACAGTGAGA ACGGGACCAT GCATGGCTTC
    GTCAACCACA CCCTCTCTTC CTTCAACGTC AGCGACTTCC AGAACGGCAC GGCCCCCAAC
    GACCCCCTGG ATCTGGGCTA CGAGGTGCAG ATCTGCAGGT ACAAAGACTA CCGGGAGCCG
    CCATGGTCGG AACACAAGTA TGACATCTCC AAGGACTTCT GGGCCGTCCT GGCCGCCCGG
    CTGGCCTTTG TCATCGTCTT CCAGAACCTG GTCATGTTCA TGAGCGACTT TGTGGACTGG
    GTCATCCCCG ACATCCCCAA GGACATCAGC CAGCAGATCC ACAAAGAGAA GGTGCTCATG
    GTGGAGCTGT TCATGCGGGA GGAGCAGGGC AAGCAGCAGC TGCTGGACAC GTGGATGGAG
    AAGGACCGGA AGAAGGCGGA GCCCTGCAAC AACCACAGCC CCGCCCCAGC CGCCCCGCCC
    CTGCCCCCGC CCCCGGAAGG CCCCGACTTC CCCGGAGGCT CCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52110
    Clone ID Related Accession (Same CDS sequence) XM_024987200.1
    Accession Version XM_024987200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAA GCGCCGGCAC
    CTTCCGGAGG AGCCCACAAA CAAGTGGAGG CAGAGGGTTA AGACCGCCAT GGCGGGGGTG
    AAATTGACAG ACAAGGTGAA GCTGACCTGG AGAGACCGGT TCCCTGCCTA CTTCACCAAT
    TTGGTGTCTA TCATCTTCAT GATCGCCGTG ACCTTTGCCA TTGTCCTTGG CGTCATCATC
    TACAGAATCT CCACGGCCGC CGCCTTGGCC ATGAACTCCT CCCCGTCCGT GCGGTCCAAC
    ATCCGGGTCA CGGTCACAGC CACCGCGGTC ATCATCAACC TGGTGGTCAT CATTCTCCTG
    GACGAGGTGT ACGGCTGCAT AGCCAGATGG CTCACCAAGA TAGAGGTTCC AAAGACGGAG
    AAGAGCTTTG AGGAGAGGCT GATCTTCAAG GCTTTCCTGC TCAAGTTCGT CAACTCCTAC
    ACACCCATCT TCTACGTGGC TTTCTTCAAA GGCCGGTTTG TCGGACGCCC GGGTGACTAC
    GTGTACATTT TCCGATCTTT CCGGATGGAG GAGTGCGCCC CGGGGGGCTG CCTGATGGAG
    CTCTGCATCC AGCTCAGCAT CATCATGCTG GGAAAGCAGC TGATCCAGAA CAACCTGTTT
    GAGATCGGCA TCCCGAAAAT GAAGAAGTTC ATCCGCTATC TGAGGCTGAA GCACCAGAGC
    CCCTCGGACC ACGACGAGCA CGTGAAGAAG AAGCAGCGGT ACGAGGTGGA CTACACGCTG
    GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC ATGGAGATGA TCATCCAGTT CGGCTTCGTC
    ACCCTGTTTG TCGCATCCTT CCCGCTGGCC CCACTCTTCG CGCTCCTGAA CAACATCATT
    GAGATCCGCC TGGACGCCAA AAAGTTTGTC ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA
    GCCAAGGACA TCGGAATCTG GTACAATATC CTCAGAGGCG TCGGGAAGCT TGCCGTCATC
    ATCAACGCGT TTGTGATCTC CTTCACGTCT GACTTCATCC CTCGCCTGGT GTACCTCTAC
    ATGTACAGTG AGAACGGGAC CATGCATGGC TTCGTCAACC ACACCCTCTC TTCCTTCAAC
    GTCAGCGACT TCCAGAACGG CACGGCCCCC AACGACCCCC TGGATCTGGG CTACGAGGTG
    CAGATCTGCA GGTACAAAGA CTACCGGGAG CCGCCATGGT CGGAACACAA GTATGACATC
    TCCAAGGACT TCTGGGCCGT CCTGGCCGCC CGGCTGGCCT TTGTCATCGT CTTCCAGAAC
    CTGGTCATGT TCATGAGCGA CTTTGTGGAC TGGGTCATCC CCGACATCCC CAAGGACATC
    AGCCAGCAGA TCCACAAAGA GAAGGTGCTC ATGGTGGAGC TGTTCATGCG GGAGGAGCAG
    GGCAAGCAGC AGCTGCTGGA CACGTGGATG GAGAAGGACC GGAAGAAGGC GGAGCCCTGC
    AACAACCACA GCCCCGCCCC AGCCGCCCCG CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC
    TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842968.1
    CDS261..3341
    Translation

    Target ORF information:

    RefSeq Version XM_024987200.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAA GCGCCGGCAC
    CTTCCGGAGG AGCCCACAAA CAAGTGGAGG CAGAGGGTTA AGACCGCCAT GGCGGGGGTG
    AAATTGACAG ACAAGGTGAA GCTGACCTGG AGAGACCGGT TCCCTGCCTA CTTCACCAAT
    TTGGTGTCTA TCATCTTCAT GATCGCCGTG ACCTTTGCCA TTGTCCTTGG CGTCATCATC
    TACAGAATCT CCACGGCCGC CGCCTTGGCC ATGAACTCCT CCCCGTCCGT GCGGTCCAAC
    ATCCGGGTCA CGGTCACAGC CACCGCGGTC ATCATCAACC TGGTGGTCAT CATTCTCCTG
    GACGAGGTGT ACGGCTGCAT AGCCAGATGG CTCACCAAGA TAGAGGTTCC AAAGACGGAG
    AAGAGCTTTG AGGAGAGGCT GATCTTCAAG GCTTTCCTGC TCAAGTTCGT CAACTCCTAC
    ACACCCATCT TCTACGTGGC TTTCTTCAAA GGCCGGTTTG TCGGACGCCC GGGTGACTAC
    GTGTACATTT TCCGATCTTT CCGGATGGAG GAGTGCGCCC CGGGGGGCTG CCTGATGGAG
    CTCTGCATCC AGCTCAGCAT CATCATGCTG GGAAAGCAGC TGATCCAGAA CAACCTGTTT
    GAGATCGGCA TCCCGAAAAT GAAGAAGTTC ATCCGCTATC TGAGGCTGAA GCACCAGAGC
    CCCTCGGACC ACGACGAGCA CGTGAAGAAG AAGCAGCGGT ACGAGGTGGA CTACACGCTG
    GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC ATGGAGATGA TCATCCAGTT CGGCTTCGTC
    ACCCTGTTTG TCGCATCCTT CCCGCTGGCC CCACTCTTCG CGCTCCTGAA CAACATCATT
    GAGATCCGCC TGGACGCCAA AAAGTTTGTC ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA
    GCCAAGGACA TCGGAATCTG GTACAATATC CTCAGAGGCG TCGGGAAGCT TGCCGTCATC
    ATCAACGCGT TTGTGATCTC CTTCACGTCT GACTTCATCC CTCGCCTGGT GTACCTCTAC
    ATGTACAGTG AGAACGGGAC CATGCATGGC TTCGTCAACC ACACCCTCTC TTCCTTCAAC
    GTCAGCGACT TCCAGAACGG CACGGCCCCC AACGACCCCC TGGATCTGGG CTACGAGGTG
    CAGATCTGCA GGTACAAAGA CTACCGGGAG CCGCCATGGT CGGAACACAA GTATGACATC
    TCCAAGGACT TCTGGGCCGT CCTGGCCGCC CGGCTGGCCT TTGTCATCGT CTTCCAGAAC
    CTGGTCATGT TCATGAGCGA CTTTGTGGAC TGGGTCATCC CCGACATCCC CAAGGACATC
    AGCCAGCAGA TCCACAAAGA GAAGGTGCTC ATGGTGGAGC TGTTCATGCG GGAGGAGCAG
    GGCAAGCAGC AGCTGCTGGA CACGTGGATG GAGAAGGACC GGAAGAAGGC GGAGCCCTGC
    AACAACCACA GCCCCGCCCC AGCCGCCCCG CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC
    TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52111
    Clone ID Related Accession (Same CDS sequence) XM_015461361.2
    Accession Version XM_015461361.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461361.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGACAGAC
    AAGGTGAAGC TGACCTGGAG AGACCGGTTC CCTGCCTACT TCACCAATTT GGTGTCTATC
    ATCTTCATGA TCGCCGTGAC CTTTGCCATT GTCCTTGGCG TCATCATCTA CAGAATCTCC
    ACGGCCGCCG CCTTGGCCAT GAACTCCTCC CCGTCCGTGC GGTCCAACAT CCGGGTCACG
    GTCACAGCCA CCGCGGTCAT CATCAACCTG GTGGTCATCA TTCTCCTGGA CGAGGTGTAC
    GGCTGCATAG CCAGATGGCT CACCAAGATA GAGGTTCCAA AGACGGAGAA GAGCTTTGAG
    GAGAGGCTGA TCTTCAAGGC TTTCCTGCTC AAGTTCGTCA ACTCCTACAC ACCCATCTTC
    TACGTGGCTT TCTTCAAAGG CCGGTTTGTC GGACGCCCGG GTGACTACGT GTACATTTTC
    CGATCTTTCC GGATGGAGGA GTGCGCCCCG GGGGGCTGCC TGATGGAGCT CTGCATCCAG
    CTCAGCATCA TCATGCTGGG AAAGCAGCTG ATCCAGAACA ACCTGTTTGA GATCGGCATC
    CCGAAAATGA AGAAGTTCAT CCGCTATCTG AGGCTGAAGC ACCAGAGCCC CTCGGACCAC
    GACGAGCACG TGAAGAAGAA GCAGCGGTAC GAGGTGGACT ACACGCTGGA GCCCTTCGCG
    GGCCTCACCC CGGAGTACAT GGAGATGATC ATCCAGTTCG GCTTCGTCAC CCTGTTTGTC
    GCATCCTTCC CGCTGGCCCC ACTCTTCGCG CTCCTGAACA ACATCATTGA GATCCGCCTG
    GACGCCAAAA AGTTTGTCAC CGAGCTCCGA AGGCCGGTGG CTGTCAGAGC CAAGGACATC
    GGAATCTGGT ACAATATCCT CAGAGGCGTC GGGAAGCTTG CCGTCATCAT CAACGCGTTT
    GTGATCTCCT TCACGTCTGA CTTCATCCCT CGCCTGGTGT ACCTCTACAT GTACAGTGAG
    AACGGGACCA TGCATGGCTT CGTCAACCAC ACCCTCTCTT CCTTCAACGT CAGCGACTTC
    CAGAACGGCA CGGCCCCCAA CGACCCCCTG GATCTGGGCT ACGAGGTGCA GATCTGCAGG
    TACAAAGACT ACCGGGAGCC GCCATGGTCG GAACACAAGT ATGACATCTC CAAGGACTTC
    TGGGCCGTCC TGGCCGCCCG GCTGGCCTTT GTCATCGTCT TCCAGAACCT GGTCATGTTC
    ATGAGCGACT TTGTGGACTG GGTCATCCCC GACATCCCCA AGGACATCAG CCAGCAGATC
    CACAAAGAGA AGGTGCTCAT GGTGGAGCTG TTCATGCGGG AGGAGCAGGG CAAGCAGCAG
    CTGCTGGACA CGTGGATGGA GAAGGACCGG AAGAAGGCGG AGCCCTGCAA CAACCACAGC
    CCCGCCCCAG CCGCCCCGCC CCTGCCCCCG CCCCCGGAAG GCCCCGACTT CCCCGGAGGC
    TCCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316847.2
    CDS260..3328
    Translation

    Target ORF information:

    RefSeq Version XM_015461361.2
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GAGGCTGTCA AGGACCATCC CAGAGCTGAA
    TATGAAGCCC GAGTTCTGGA GAAGTCTCTT AGGAAAGAAT CCAAAAACAA AGAGACAGAC
    AAGGTGAAGC TGACCTGGAG AGACCGGTTC CCTGCCTACT TCACCAATTT GGTGTCTATC
    ATCTTCATGA TCGCCGTGAC CTTTGCCATT GTCCTTGGCG TCATCATCTA CAGAATCTCC
    ACGGCCGCCG CCTTGGCCAT GAACTCCTCC CCGTCCGTGC GGTCCAACAT CCGGGTCACG
    GTCACAGCCA CCGCGGTCAT CATCAACCTG GTGGTCATCA TTCTCCTGGA CGAGGTGTAC
    GGCTGCATAG CCAGATGGCT CACCAAGATA GAGGTTCCAA AGACGGAGAA GAGCTTTGAG
    GAGAGGCTGA TCTTCAAGGC TTTCCTGCTC AAGTTCGTCA ACTCCTACAC ACCCATCTTC
    TACGTGGCTT TCTTCAAAGG CCGGTTTGTC GGACGCCCGG GTGACTACGT GTACATTTTC
    CGATCTTTCC GGATGGAGGA GTGCGCCCCG GGGGGCTGCC TGATGGAGCT CTGCATCCAG
    CTCAGCATCA TCATGCTGGG AAAGCAGCTG ATCCAGAACA ACCTGTTTGA GATCGGCATC
    CCGAAAATGA AGAAGTTCAT CCGCTATCTG AGGCTGAAGC ACCAGAGCCC CTCGGACCAC
    GACGAGCACG TGAAGAAGAA GCAGCGGTAC GAGGTGGACT ACACGCTGGA GCCCTTCGCG
    GGCCTCACCC CGGAGTACAT GGAGATGATC ATCCAGTTCG GCTTCGTCAC CCTGTTTGTC
    GCATCCTTCC CGCTGGCCCC ACTCTTCGCG CTCCTGAACA ACATCATTGA GATCCGCCTG
    GACGCCAAAA AGTTTGTCAC CGAGCTCCGA AGGCCGGTGG CTGTCAGAGC CAAGGACATC
    GGAATCTGGT ACAATATCCT CAGAGGCGTC GGGAAGCTTG CCGTCATCAT CAACGCGTTT
    GTGATCTCCT TCACGTCTGA CTTCATCCCT CGCCTGGTGT ACCTCTACAT GTACAGTGAG
    AACGGGACCA TGCATGGCTT CGTCAACCAC ACCCTCTCTT CCTTCAACGT CAGCGACTTC
    CAGAACGGCA CGGCCCCCAA CGACCCCCTG GATCTGGGCT ACGAGGTGCA GATCTGCAGG
    TACAAAGACT ACCGGGAGCC GCCATGGTCG GAACACAAGT ATGACATCTC CAAGGACTTC
    TGGGCCGTCC TGGCCGCCCG GCTGGCCTTT GTCATCGTCT TCCAGAACCT GGTCATGTTC
    ATGAGCGACT TTGTGGACTG GGTCATCCCC GACATCCCCA AGGACATCAG CCAGCAGATC
    CACAAAGAGA AGGTGCTCAT GGTGGAGCTG TTCATGCGGG AGGAGCAGGG CAAGCAGCAG
    CTGCTGGACA CGTGGATGGA GAAGGACCGG AAGAAGGCGG AGCCCTGCAA CAACCACAGC
    CCCGCCCCAG CCGCCCCGCC CCTGCCCCCG CCCCCGGAAG GCCCCGACTT CCCCGGAGGC
    TCCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52112
    Clone ID Related Accession (Same CDS sequence) XM_024987202.1
    Accession Version XM_024987202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GACCATCCCA GAGCTGAATA TGAAGCCCGA
    GTTCTGGAGA AGTCTCTTAG GAAAGAATCC AAAAACAAAG AGACAGACAA GGTGAAGCTG
    ACCTGGAGAG ACCGGTTCCC TGCCTACTTC ACCAATTTGG TGTCTATCAT CTTCATGATC
    GCCGTGACCT TTGCCATTGT CCTTGGCGTC ATCATCTACA GAATCTCCAC GGCCGCCGCC
    TTGGCCATGA ACTCCTCCCC GTCCGTGCGG TCCAACATCC GGGTCACGGT CACAGCCACC
    GCGGTCATCA TCAACCTGGT GGTCATCATT CTCCTGGACG AGGTGTACGG CTGCATAGCC
    AGATGGCTCA CCAAGATAGA GGTTCCAAAG ACGGAGAAGA GCTTTGAGGA GAGGCTGATC
    TTCAAGGCTT TCCTGCTCAA GTTCGTCAAC TCCTACACAC CCATCTTCTA CGTGGCTTTC
    TTCAAAGGCC GGTTTGTCGG ACGCCCGGGT GACTACGTGT ACATTTTCCG ATCTTTCCGG
    ATGGAGGAGT GCGCCCCGGG GGGCTGCCTG ATGGAGCTCT GCATCCAGCT CAGCATCATC
    ATGCTGGGAA AGCAGCTGAT CCAGAACAAC CTGTTTGAGA TCGGCATCCC GAAAATGAAG
    AAGTTCATCC GCTATCTGAG GCTGAAGCAC CAGAGCCCCT CGGACCACGA CGAGCACGTG
    AAGAAGAAGC AGCGGTACGA GGTGGACTAC ACGCTGGAGC CCTTCGCGGG CCTCACCCCG
    GAGTACATGG AGATGATCAT CCAGTTCGGC TTCGTCACCC TGTTTGTCGC ATCCTTCCCG
    CTGGCCCCAC TCTTCGCGCT CCTGAACAAC ATCATTGAGA TCCGCCTGGA CGCCAAAAAG
    TTTGTCACCG AGCTCCGAAG GCCGGTGGCT GTCAGAGCCA AGGACATCGG AATCTGGTAC
    AATATCCTCA GAGGCGTCGG GAAGCTTGCC GTCATCATCA ACGCGTTTGT GATCTCCTTC
    ACGTCTGACT TCATCCCTCG CCTGGTGTAC CTCTACATGT ACAGTGAGAA CGGGACCATG
    CATGGCTTCG TCAACCACAC CCTCTCTTCC TTCAACGTCA GCGACTTCCA GAACGGCACG
    GCCCCCAACG ACCCCCTGGA TCTGGGCTAC GAGGTGCAGA TCTGCAGGTA CAAAGACTAC
    CGGGAGCCGC CATGGTCGGA ACACAAGTAT GACATCTCCA AGGACTTCTG GGCCGTCCTG
    GCCGCCCGGC TGGCCTTTGT CATCGTCTTC CAGAACCTGG TCATGTTCAT GAGCGACTTT
    GTGGACTGGG TCATCCCCGA CATCCCCAAG GACATCAGCC AGCAGATCCA CAAAGAGAAG
    GTGCTCATGG TGGAGCTGTT CATGCGGGAG GAGCAGGGCA AGCAGCAGCT GCTGGACACG
    TGGATGGAGA AGGACCGGAA GAAGGCGGAG CCCTGCAACA ACCACAGCCC CGCCCCAGCC
    GCCCCGCCCC TGCCCCCGCC CCCGGAAGGC CCCGACTTCC CCGGAGGCTC CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842970.1
    CDS260..3316
    Translation

    Target ORF information:

    RefSeq Version XM_024987202.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGGCAAGGA GAGGGAAGAA AGAAGGACTC TGCCCTTTTA
    AATAAGAGGC GCAAGTGTGA TAGATACGGC ATCACGAGCC TGCTGGCCAA TGGTGTCTAC
    TCGGCGGCCT ACCCGCTGCA TGATGGAGAC TATGAAGGAG ACAACGTGGA GTTCAATGAC
    AGGAAGCTCC TGTACGAAGA GTGGGCGAGT TACGGGGTCT TCTACAAGTA CCAGCCCATC
    GACCTCGTCA GAAAGTATTT TGGGGAGAAG ATTGGTCTGT ACTTCGCCTG GCTTGGCGTC
    TACACCCAGA TGCTCATCCC GGCCTCCGTG GTCGGCATCA TCGTTTTCCT CTACGGATGG
    GCCACTGTCG ATGACAACAT CCCCAGCATG GAGATGTGCG ACCAGAGGCA CAACATCACC
    ATGTGCCCGC TCTGCGACAA GACCTGCAGC TACTGGAAGA TGAGCTCCGC GTGCGCCACC
    GCCCGCGCCA GCCACCTCTT CGACAACCCC GCCACTGTCT TCTTCTCCAT CTTCATGGCC
    CTCTGGGCTG CCACCTTCAT GGAGCACTGG AAGCGGAAAC AGATGCGGCT CAATTACCGG
    TGGGACCTCA CAGGCTTCGA GGAGGAGGAG GACCATCCCA GAGCTGAATA TGAAGCCCGA
    GTTCTGGAGA AGTCTCTTAG GAAAGAATCC AAAAACAAAG AGACAGACAA GGTGAAGCTG
    ACCTGGAGAG ACCGGTTCCC TGCCTACTTC ACCAATTTGG TGTCTATCAT CTTCATGATC
    GCCGTGACCT TTGCCATTGT CCTTGGCGTC ATCATCTACA GAATCTCCAC GGCCGCCGCC
    TTGGCCATGA ACTCCTCCCC GTCCGTGCGG TCCAACATCC GGGTCACGGT CACAGCCACC
    GCGGTCATCA TCAACCTGGT GGTCATCATT CTCCTGGACG AGGTGTACGG CTGCATAGCC
    AGATGGCTCA CCAAGATAGA GGTTCCAAAG ACGGAGAAGA GCTTTGAGGA GAGGCTGATC
    TTCAAGGCTT TCCTGCTCAA GTTCGTCAAC TCCTACACAC CCATCTTCTA CGTGGCTTTC
    TTCAAAGGCC GGTTTGTCGG ACGCCCGGGT GACTACGTGT ACATTTTCCG ATCTTTCCGG
    ATGGAGGAGT GCGCCCCGGG GGGCTGCCTG ATGGAGCTCT GCATCCAGCT CAGCATCATC
    ATGCTGGGAA AGCAGCTGAT CCAGAACAAC CTGTTTGAGA TCGGCATCCC GAAAATGAAG
    AAGTTCATCC GCTATCTGAG GCTGAAGCAC CAGAGCCCCT CGGACCACGA CGAGCACGTG
    AAGAAGAAGC AGCGGTACGA GGTGGACTAC ACGCTGGAGC CCTTCGCGGG CCTCACCCCG
    GAGTACATGG AGATGATCAT CCAGTTCGGC TTCGTCACCC TGTTTGTCGC ATCCTTCCCG
    CTGGCCCCAC TCTTCGCGCT CCTGAACAAC ATCATTGAGA TCCGCCTGGA CGCCAAAAAG
    TTTGTCACCG AGCTCCGAAG GCCGGTGGCT GTCAGAGCCA AGGACATCGG AATCTGGTAC
    AATATCCTCA GAGGCGTCGG GAAGCTTGCC GTCATCATCA ACGCGTTTGT GATCTCCTTC
    ACGTCTGACT TCATCCCTCG CCTGGTGTAC CTCTACATGT ACAGTGAGAA CGGGACCATG
    CATGGCTTCG TCAACCACAC CCTCTCTTCC TTCAACGTCA GCGACTTCCA GAACGGCACG
    GCCCCCAACG ACCCCCTGGA TCTGGGCTAC GAGGTGCAGA TCTGCAGGTA CAAAGACTAC
    CGGGAGCCGC CATGGTCGGA ACACAAGTAT GACATCTCCA AGGACTTCTG GGCCGTCCTG
    GCCGCCCGGC TGGCCTTTGT CATCGTCTTC CAGAACCTGG TCATGTTCAT GAGCGACTTT
    GTGGACTGGG TCATCCCCGA CATCCCCAAG GACATCAGCC AGCAGATCCA CAAAGAGAAG
    GTGCTCATGG TGGAGCTGTT CATGCGGGAG GAGCAGGGCA AGCAGCAGCT GCTGGACACG
    TGGATGGAGA AGGACCGGAA GAAGGCGGAG CCCTGCAACA ACCACAGCCC CGCCCCAGCC
    GCCCCGCCCC TGCCCCCGCC CCCGGAAGGC CCCGACTTCC CCGGAGGCTC CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52113
    Clone ID Related Accession (Same CDS sequence) XM_015461362.2
    Accession Version XM_015461362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAC AGACAAGGTG
    AAGCTGACCT GGAGAGACCG GTTCCCTGCC TACTTCACCA ATTTGGTGTC TATCATCTTC
    ATGATCGCCG TGACCTTTGC CATTGTCCTT GGCGTCATCA TCTACAGAAT CTCCACGGCC
    GCCGCCTTGG CCATGAACTC CTCCCCGTCC GTGCGGTCCA ACATCCGGGT CACGGTCACA
    GCCACCGCGG TCATCATCAA CCTGGTGGTC ATCATTCTCC TGGACGAGGT GTACGGCTGC
    ATAGCCAGAT GGCTCACCAA GATAGAGGTT CCAAAGACGG AGAAGAGCTT TGAGGAGAGG
    CTGATCTTCA AGGCTTTCCT GCTCAAGTTC GTCAACTCCT ACACACCCAT CTTCTACGTG
    GCTTTCTTCA AAGGCCGGTT TGTCGGACGC CCGGGTGACT ACGTGTACAT TTTCCGATCT
    TTCCGGATGG AGGAGTGCGC CCCGGGGGGC TGCCTGATGG AGCTCTGCAT CCAGCTCAGC
    ATCATCATGC TGGGAAAGCA GCTGATCCAG AACAACCTGT TTGAGATCGG CATCCCGAAA
    ATGAAGAAGT TCATCCGCTA TCTGAGGCTG AAGCACCAGA GCCCCTCGGA CCACGACGAG
    CACGTGAAGA AGAAGCAGCG GTACGAGGTG GACTACACGC TGGAGCCCTT CGCGGGCCTC
    ACCCCGGAGT ACATGGAGAT GATCATCCAG TTCGGCTTCG TCACCCTGTT TGTCGCATCC
    TTCCCGCTGG CCCCACTCTT CGCGCTCCTG AACAACATCA TTGAGATCCG CCTGGACGCC
    AAAAAGTTTG TCACCGAGCT CCGAAGGCCG GTGGCTGTCA GAGCCAAGGA CATCGGAATC
    TGGTACAATA TCCTCAGAGG CGTCGGGAAG CTTGCCGTCA TCATCAACGC GTTTGTGATC
    TCCTTCACGT CTGACTTCAT CCCTCGCCTG GTGTACCTCT ACATGTACAG TGAGAACGGG
    ACCATGCATG GCTTCGTCAA CCACACCCTC TCTTCCTTCA ACGTCAGCGA CTTCCAGAAC
    GGCACGGCCC CCAACGACCC CCTGGATCTG GGCTACGAGG TGCAGATCTG CAGGTACAAA
    GACTACCGGG AGCCGCCATG GTCGGAACAC AAGTATGACA TCTCCAAGGA CTTCTGGGCC
    GTCCTGGCCG CCCGGCTGGC CTTTGTCATC GTCTTCCAGA ACCTGGTCAT GTTCATGAGC
    GACTTTGTGG ACTGGGTCAT CCCCGACATC CCCAAGGACA TCAGCCAGCA GATCCACAAA
    GAGAAGGTGC TCATGGTGGA GCTGTTCATG CGGGAGGAGC AGGGCAAGCA GCAGCTGCTG
    GACACGTGGA TGGAGAAGGA CCGGAAGAAG GCGGAGCCCT GCAACAACCA CAGCCCCGCC
    CCAGCCGCCC CGCCCCTGCC CCCGCCCCCG GAAGGCCCCG ACTTCCCCGG AGGCTCCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316848.2
    CDS259..3261
    Translation

    Target ORF information:

    RefSeq Version XM_015461362.2
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGAGGCT GTCAAGGACC ATCCCAGAGC TGAATATGAA
    GCCCGAGTTC TGGAGAAGTC TCTTAGGAAA GAATCCAAAA ACAAAGAGAC AGACAAGGTG
    AAGCTGACCT GGAGAGACCG GTTCCCTGCC TACTTCACCA ATTTGGTGTC TATCATCTTC
    ATGATCGCCG TGACCTTTGC CATTGTCCTT GGCGTCATCA TCTACAGAAT CTCCACGGCC
    GCCGCCTTGG CCATGAACTC CTCCCCGTCC GTGCGGTCCA ACATCCGGGT CACGGTCACA
    GCCACCGCGG TCATCATCAA CCTGGTGGTC ATCATTCTCC TGGACGAGGT GTACGGCTGC
    ATAGCCAGAT GGCTCACCAA GATAGAGGTT CCAAAGACGG AGAAGAGCTT TGAGGAGAGG
    CTGATCTTCA AGGCTTTCCT GCTCAAGTTC GTCAACTCCT ACACACCCAT CTTCTACGTG
    GCTTTCTTCA AAGGCCGGTT TGTCGGACGC CCGGGTGACT ACGTGTACAT TTTCCGATCT
    TTCCGGATGG AGGAGTGCGC CCCGGGGGGC TGCCTGATGG AGCTCTGCAT CCAGCTCAGC
    ATCATCATGC TGGGAAAGCA GCTGATCCAG AACAACCTGT TTGAGATCGG CATCCCGAAA
    ATGAAGAAGT TCATCCGCTA TCTGAGGCTG AAGCACCAGA GCCCCTCGGA CCACGACGAG
    CACGTGAAGA AGAAGCAGCG GTACGAGGTG GACTACACGC TGGAGCCCTT CGCGGGCCTC
    ACCCCGGAGT ACATGGAGAT GATCATCCAG TTCGGCTTCG TCACCCTGTT TGTCGCATCC
    TTCCCGCTGG CCCCACTCTT CGCGCTCCTG AACAACATCA TTGAGATCCG CCTGGACGCC
    AAAAAGTTTG TCACCGAGCT CCGAAGGCCG GTGGCTGTCA GAGCCAAGGA CATCGGAATC
    TGGTACAATA TCCTCAGAGG CGTCGGGAAG CTTGCCGTCA TCATCAACGC GTTTGTGATC
    TCCTTCACGT CTGACTTCAT CCCTCGCCTG GTGTACCTCT ACATGTACAG TGAGAACGGG
    ACCATGCATG GCTTCGTCAA CCACACCCTC TCTTCCTTCA ACGTCAGCGA CTTCCAGAAC
    GGCACGGCCC CCAACGACCC CCTGGATCTG GGCTACGAGG TGCAGATCTG CAGGTACAAA
    GACTACCGGG AGCCGCCATG GTCGGAACAC AAGTATGACA TCTCCAAGGA CTTCTGGGCC
    GTCCTGGCCG CCCGGCTGGC CTTTGTCATC GTCTTCCAGA ACCTGGTCAT GTTCATGAGC
    GACTTTGTGG ACTGGGTCAT CCCCGACATC CCCAAGGACA TCAGCCAGCA GATCCACAAA
    GAGAAGGTGC TCATGGTGGA GCTGTTCATG CGGGAGGAGC AGGGCAAGCA GCAGCTGCTG
    GACACGTGGA TGGAGAAGGA CCGGAAGAAG GCGGAGCCCT GCAACAACCA CAGCCCCGCC
    CCAGCCGCCC CGCCCCTGCC CCCGCCCCCG GAAGGCCCCG ACTTCCCCGG AGGCTCCCTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52114
    Clone ID Related Accession (Same CDS sequence) XM_024987204.1
    Accession Version XM_024987204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2991bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGACCAT CCCAGAGCTG AATATGAAGC CCGAGTTCTG
    GAGAAGTCTC TTAGGAAAGA ATCCAAAAAC AAAGAGACAG ACAAGGTGAA GCTGACCTGG
    AGAGACCGGT TCCCTGCCTA CTTCACCAAT TTGGTGTCTA TCATCTTCAT GATCGCCGTG
    ACCTTTGCCA TTGTCCTTGG CGTCATCATC TACAGAATCT CCACGGCCGC CGCCTTGGCC
    ATGAACTCCT CCCCGTCCGT GCGGTCCAAC ATCCGGGTCA CGGTCACAGC CACCGCGGTC
    ATCATCAACC TGGTGGTCAT CATTCTCCTG GACGAGGTGT ACGGCTGCAT AGCCAGATGG
    CTCACCAAGA TAGAGGTTCC AAAGACGGAG AAGAGCTTTG AGGAGAGGCT GATCTTCAAG
    GCTTTCCTGC TCAAGTTCGT CAACTCCTAC ACACCCATCT TCTACGTGGC TTTCTTCAAA
    GGCCGGTTTG TCGGACGCCC GGGTGACTAC GTGTACATTT TCCGATCTTT CCGGATGGAG
    GAGTGCGCCC CGGGGGGCTG CCTGATGGAG CTCTGCATCC AGCTCAGCAT CATCATGCTG
    GGAAAGCAGC TGATCCAGAA CAACCTGTTT GAGATCGGCA TCCCGAAAAT GAAGAAGTTC
    ATCCGCTATC TGAGGCTGAA GCACCAGAGC CCCTCGGACC ACGACGAGCA CGTGAAGAAG
    AAGCAGCGGT ACGAGGTGGA CTACACGCTG GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC
    ATGGAGATGA TCATCCAGTT CGGCTTCGTC ACCCTGTTTG TCGCATCCTT CCCGCTGGCC
    CCACTCTTCG CGCTCCTGAA CAACATCATT GAGATCCGCC TGGACGCCAA AAAGTTTGTC
    ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA GCCAAGGACA TCGGAATCTG GTACAATATC
    CTCAGAGGCG TCGGGAAGCT TGCCGTCATC ATCAACGCGT TTGTGATCTC CTTCACGTCT
    GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTG AGAACGGGAC CATGCATGGC
    TTCGTCAACC ACACCCTCTC TTCCTTCAAC GTCAGCGACT TCCAGAACGG CACGGCCCCC
    AACGACCCCC TGGATCTGGG CTACGAGGTG CAGATCTGCA GGTACAAAGA CTACCGGGAG
    CCGCCATGGT CGGAACACAA GTATGACATC TCCAAGGACT TCTGGGCCGT CCTGGCCGCC
    CGGCTGGCCT TTGTCATCGT CTTCCAGAAC CTGGTCATGT TCATGAGCGA CTTTGTGGAC
    TGGGTCATCC CCGACATCCC CAAGGACATC AGCCAGCAGA TCCACAAAGA GAAGGTGCTC
    ATGGTGGAGC TGTTCATGCG GGAGGAGCAG GGCAAGCAGC AGCTGCTGGA CACGTGGATG
    GAGAAGGACC GGAAGAAGGC GGAGCCCTGC AACAACCACA GCCCCGCCCC AGCCGCCCCG
    CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842972.1
    CDS259..3249
    Translation

    Target ORF information:

    RefSeq Version XM_024987204.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGGAGG ACGACATCGG CCTCGAGGCA TTGACCCCCA AGGAAGCCCC CGCAGCTGAG 
    GCCGCCGGGG CCGTGGATGG GGAGGGCGCG CCGCCCGGCG GTCCCGGCGC ACAGGCGGCC
    GCGATGAGGG TCAACGAGAA GTACTCGACG CTCCCGGCCG AGGACCGCAG CGTCCACATC
    ATCAACATCT GCGCCATCGA GGACATCGGC TACCTGCCGT CCGAGGGCAC GCTGCTGAAC
    TCCTTGTCCG TGGACCCGGA CGCCGAGTGC AAGTACGGCC TGTACTTCCG CGACGGCAAG
    CGCAAGGTGG ACTATGTCCT GGTGTACCAT CACAAGAGGG CCTCGGGCGG CAGGACGCTG
    GCCAGGAGGG CCCAGCACGG CGACGCGGGC CCGGCCGCCC GCAGCGCCCG GCAGGACCAG
    CCCCTGCCCG GGAAGGCGGG CCCCCTGGGC GCGGGCGAGC CCGAGCCGCC GCTGGACTAC
    CACGAAGATG ACAAGCGCTT CCGCCGGGAG GAGTACGAGG GCAACCTGGT GGAGGCCGGC
    CTGGAGCTGG AGCGGGACGA GGACACAAAA ATTCACGGGG TTGGATTTGT AAAAATCCAC
    GCACCCTGGA ATGTGCTGTG CCGAGAAGCC GAGTTTTTGA AGCTGAAGAT GCCGACAAAG
    AAGCTGTATC ACATCAGCGA GACCCGCGGC CTCCTCAAGA AGATCAACTC TGTGCTCCAG
    AAGATCACGG ACCCCATCCA GCCCAAGGTG GCAGAGCACA GGCCCCAGAC CACCAAGAGG
    CTCTCCTACC CCTTTTCCCG GGAGAAGCAG CACCTATTTG ACCTGTCTGA CAAGGATTCC
    TTTTTCGACA GCAAAACTCG GAGCACAATA GTCTACGAAA TCCTGAAGAG AACAACGTGT
    ACGAAGGCCA AGTACAGCAT GGGCATCACG AGCCTGCTGG CCAATGGTGT CTACTCGGCG
    GCCTACCCGC TGCATGATGG AGACTATGAA GGAGACAACG TGGAGTTCAA TGACAGGAAG
    CTCCTGTACG AAGAGTGGGC GAGTTACGGG GTCTTCTACA AGTACCAGCC CATCGACCTC
    GTCAGAAAGT ATTTTGGGGA GAAGATTGGT CTGTACTTCG CCTGGCTTGG CGTCTACACC
    CAGATGCTCA TCCCGGCCTC CGTGGTCGGC ATCATCGTTT TCCTCTACGG ATGGGCCACT
    GTCGATGACA ACATCCCCAG CATGGAGATG TGCGACCAGA GGCACAACAT CACCATGTGC
    CCGCTCTGCG ACAAGACCTG CAGCTACTGG AAGATGAGCT CCGCGTGCGC CACCGCCCGC
    GCCAGCCACC TCTTCGACAA CCCCGCCACT GTCTTCTTCT CCATCTTCAT GGCCCTCTGG
    GCTGCCACCT TCATGGAGCA CTGGAAGCGG AAACAGATGC GGCTCAATTA CCGGTGGGAC
    CTCACAGGCT TCGAGGAGGA GGAGGACCAT CCCAGAGCTG AATATGAAGC CCGAGTTCTG
    GAGAAGTCTC TTAGGAAAGA ATCCAAAAAC AAAGAGACAG ACAAGGTGAA GCTGACCTGG
    AGAGACCGGT TCCCTGCCTA CTTCACCAAT TTGGTGTCTA TCATCTTCAT GATCGCCGTG
    ACCTTTGCCA TTGTCCTTGG CGTCATCATC TACAGAATCT CCACGGCCGC CGCCTTGGCC
    ATGAACTCCT CCCCGTCCGT GCGGTCCAAC ATCCGGGTCA CGGTCACAGC CACCGCGGTC
    ATCATCAACC TGGTGGTCAT CATTCTCCTG GACGAGGTGT ACGGCTGCAT AGCCAGATGG
    CTCACCAAGA TAGAGGTTCC AAAGACGGAG AAGAGCTTTG AGGAGAGGCT GATCTTCAAG
    GCTTTCCTGC TCAAGTTCGT CAACTCCTAC ACACCCATCT TCTACGTGGC TTTCTTCAAA
    GGCCGGTTTG TCGGACGCCC GGGTGACTAC GTGTACATTT TCCGATCTTT CCGGATGGAG
    GAGTGCGCCC CGGGGGGCTG CCTGATGGAG CTCTGCATCC AGCTCAGCAT CATCATGCTG
    GGAAAGCAGC TGATCCAGAA CAACCTGTTT GAGATCGGCA TCCCGAAAAT GAAGAAGTTC
    ATCCGCTATC TGAGGCTGAA GCACCAGAGC CCCTCGGACC ACGACGAGCA CGTGAAGAAG
    AAGCAGCGGT ACGAGGTGGA CTACACGCTG GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC
    ATGGAGATGA TCATCCAGTT CGGCTTCGTC ACCCTGTTTG TCGCATCCTT CCCGCTGGCC
    CCACTCTTCG CGCTCCTGAA CAACATCATT GAGATCCGCC TGGACGCCAA AAAGTTTGTC
    ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA GCCAAGGACA TCGGAATCTG GTACAATATC
    CTCAGAGGCG TCGGGAAGCT TGCCGTCATC ATCAACGCGT TTGTGATCTC CTTCACGTCT
    GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTG AGAACGGGAC CATGCATGGC
    TTCGTCAACC ACACCCTCTC TTCCTTCAAC GTCAGCGACT TCCAGAACGG CACGGCCCCC
    AACGACCCCC TGGATCTGGG CTACGAGGTG CAGATCTGCA GGTACAAAGA CTACCGGGAG
    CCGCCATGGT CGGAACACAA GTATGACATC TCCAAGGACT TCTGGGCCGT CCTGGCCGCC
    CGGCTGGCCT TTGTCATCGT CTTCCAGAAC CTGGTCATGT TCATGAGCGA CTTTGTGGAC
    TGGGTCATCC CCGACATCCC CAAGGACATC AGCCAGCAGA TCCACAAAGA GAAGGTGCTC
    ATGGTGGAGC TGTTCATGCG GGAGGAGCAG GGCAAGCAGC AGCTGCTGGA CACGTGGATG
    GAGAAGGACC GGAAGAAGGC GGAGCCCTGC AACAACCACA GCCCCGCCCC AGCCGCCCCG
    CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52115
    Clone ID Related Accession (Same CDS sequence) XM_024987203.1
    Accession Version XM_024987203.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GAAGCGCCGG
    CACCTTCCGG AGGAGCCCAC AAACAAGTGG AGGCAGAGGG TTAAGACCGC CATGGCGGGG
    GTGAAATTGA TCCACCCCAT TCTAGAAACA TCCCAGACAG ACAAGGTGAA GCTGACCTGG
    AGAGACCGGT TCCCTGCCTA CTTCACCAAT TTGGTGTCTA TCATCTTCAT GATCGCCGTG
    ACCTTTGCCA TTGTCCTTGG CGTCATCATC TACAGAATCT CCACGGCCGC CGCCTTGGCC
    ATGAACTCCT CCCCGTCCGT GCGGTCCAAC ATCCGGGTCA CGGTCACAGC CACCGCGGTC
    ATCATCAACC TGGTGGTCAT CATTCTCCTG GACGAGGTGT ACGGCTGCAT AGCCAGATGG
    CTCACCAAGA TAGAGGTTCC AAAGACGGAG AAGAGCTTTG AGGAGAGGCT GATCTTCAAG
    GCTTTCCTGC TCAAGTTCGT CAACTCCTAC ACACCCATCT TCTACGTGGC TTTCTTCAAA
    GGCCGGTTTG TCGGACGCCC GGGTGACTAC GTGTACATTT TCCGATCTTT CCGGATGGAG
    GAGTGCGCCC CGGGGGGCTG CCTGATGGAG CTCTGCATCC AGCTCAGCAT CATCATGCTG
    GGAAAGCAGC TGATCCAGAA CAACCTGTTT GAGATCGGCA TCCCGAAAAT GAAGAAGTTC
    ATCCGCTATC TGAGGCTGAA GCACCAGAGC CCCTCGGACC ACGACGAGCA CGTGAAGAAG
    AAGCAGCGGT ACGAGGTGGA CTACACGCTG GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC
    ATGGAGATGA TCATCCAGTT CGGCTTCGTC ACCCTGTTTG TCGCATCCTT CCCGCTGGCC
    CCACTCTTCG CGCTCCTGAA CAACATCATT GAGATCCGCC TGGACGCCAA AAAGTTTGTC
    ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA GCCAAGGACA TCGGAATCTG GTACAATATC
    CTCAGAGGCG TCGGGAAGCT TGCCGTCATC ATCAACGCGT TTGTGATCTC CTTCACGTCT
    GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTG AGAACGGGAC CATGCATGGC
    TTCGTCAACC ACACCCTCTC TTCCTTCAAC GTCAGCGACT TCCAGAACGG CACGGCCCCC
    AACGACCCCC TGGATCTGGG CTACGAGGTG CAGATCTGCA GGTACAAAGA CTACCGGGAG
    CCGCCATGGT CGGAACACAA GTATGACATC TCCAAGGACT TCTGGGCCGT CCTGGCCGCC
    CGGCTGGCCT TTGTCATCGT CTTCCAGAAC CTGGTCATGT TCATGAGCGA CTTTGTGGAC
    TGGGTCATCC CCGACATCCC CAAGGACATC AGCCAGCAGA TCCACAAAGA GAAGGTGCTC
    ATGGTGGAGC TGTTCATGCG GGAGGAGCAG GGCAAGCAGC AGCTGCTGGA CACGTGGATG
    GAGAAGGACC GGAAGAAGGC GGAGCCCTGC AACAACCACA GCCCCGCCCC AGCCGCCCCG
    CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842971.1
    CDS151..3201
    Translation

    Target ORF information:

    RefSeq Version XM_024987203.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1 (ANO1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987203.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GAAGCGCCGG
    CACCTTCCGG AGGAGCCCAC AAACAAGTGG AGGCAGAGGG TTAAGACCGC CATGGCGGGG
    GTGAAATTGA TCCACCCCAT TCTAGAAACA TCCCAGACAG ACAAGGTGAA GCTGACCTGG
    AGAGACCGGT TCCCTGCCTA CTTCACCAAT TTGGTGTCTA TCATCTTCAT GATCGCCGTG
    ACCTTTGCCA TTGTCCTTGG CGTCATCATC TACAGAATCT CCACGGCCGC CGCCTTGGCC
    ATGAACTCCT CCCCGTCCGT GCGGTCCAAC ATCCGGGTCA CGGTCACAGC CACCGCGGTC
    ATCATCAACC TGGTGGTCAT CATTCTCCTG GACGAGGTGT ACGGCTGCAT AGCCAGATGG
    CTCACCAAGA TAGAGGTTCC AAAGACGGAG AAGAGCTTTG AGGAGAGGCT GATCTTCAAG
    GCTTTCCTGC TCAAGTTCGT CAACTCCTAC ACACCCATCT TCTACGTGGC TTTCTTCAAA
    GGCCGGTTTG TCGGACGCCC GGGTGACTAC GTGTACATTT TCCGATCTTT CCGGATGGAG
    GAGTGCGCCC CGGGGGGCTG CCTGATGGAG CTCTGCATCC AGCTCAGCAT CATCATGCTG
    GGAAAGCAGC TGATCCAGAA CAACCTGTTT GAGATCGGCA TCCCGAAAAT GAAGAAGTTC
    ATCCGCTATC TGAGGCTGAA GCACCAGAGC CCCTCGGACC ACGACGAGCA CGTGAAGAAG
    AAGCAGCGGT ACGAGGTGGA CTACACGCTG GAGCCCTTCG CGGGCCTCAC CCCGGAGTAC
    ATGGAGATGA TCATCCAGTT CGGCTTCGTC ACCCTGTTTG TCGCATCCTT CCCGCTGGCC
    CCACTCTTCG CGCTCCTGAA CAACATCATT GAGATCCGCC TGGACGCCAA AAAGTTTGTC
    ACCGAGCTCC GAAGGCCGGT GGCTGTCAGA GCCAAGGACA TCGGAATCTG GTACAATATC
    CTCAGAGGCG TCGGGAAGCT TGCCGTCATC ATCAACGCGT TTGTGATCTC CTTCACGTCT
    GACTTCATCC CTCGCCTGGT GTACCTCTAC ATGTACAGTG AGAACGGGAC CATGCATGGC
    TTCGTCAACC ACACCCTCTC TTCCTTCAAC GTCAGCGACT TCCAGAACGG CACGGCCCCC
    AACGACCCCC TGGATCTGGG CTACGAGGTG CAGATCTGCA GGTACAAAGA CTACCGGGAG
    CCGCCATGGT CGGAACACAA GTATGACATC TCCAAGGACT TCTGGGCCGT CCTGGCCGCC
    CGGCTGGCCT TTGTCATCGT CTTCCAGAAC CTGGTCATGT TCATGAGCGA CTTTGTGGAC
    TGGGTCATCC CCGACATCCC CAAGGACATC AGCCAGCAGA TCCACAAAGA GAAGGTGCTC
    ATGGTGGAGC TGTTCATGCG GGAGGAGCAG GGCAAGCAGC AGCTGCTGGA CACGTGGATG
    GAGAAGGACC GGAAGAAGGC GGAGCCCTGC AACAACCACA GCCCCGCCCC AGCCGCCCCG
    CCCCTGCCCC CGCCCCCGGA AGGCCCCGAC TTCCCCGGAG GCTCCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89163
    Clone ID Related Accession (Same CDS sequence) XM_015461360.1
    Accession Version XM_015461360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GAAGCGCCGG
    CACCTTCCGG AGGAGCCCAC AAACAAGTGG AGGCAGAGGG TTAAGACCGC CATGGCGGGG
    GTGAAATTGA CAGACAAGGT GAAGCTGACC TGGAGAGACC GGTTCCCTGC CTACTTCACC
    AATTTGGTGT CTATCATCTT CATGATCGCC GTGACCTTTG CCATTGTCCT TGGCGTCATC
    ATCTACAGAA TCTCCACGGC CGCCGCCTTG GCCATGAACT CCTCCCCGTC CGTGCGGTCC
    AACATCCGGG TCACGGTCAC AGCCACCGCG GTCATCATCA ACCTGGTGGT CATCATTCTC
    CTGGACGAGG TGTACGGCTG CATAGCCAGA TGGCTCACCA AGATAGAGGT TCCAAAGACG
    GAGAAGAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTCAAGTT CGTCAACTCC
    TACACACCCA TCTTCTACGT GGCTTTCTTC AAAGGCCGGT TTGTCGGACG CCCGGGTGAC
    TACGTGTACA TTTTCCGATC TTTCCGGATG GAGGAGTGCG CCCCGGGGGG CTGCCTGATG
    GAGCTCTGCA TCCAGCTCAG CATCATCATG CTGGGAAAGC AGCTGATCCA GAACAACCTG
    TTTGAGATCG GCATCCCGAA AATGAAGAAG TTCATCCGCT ATCTGAGGCT GAAGCACCAG
    AGCCCCTCGG ACCACGACGA GCACGTGAAG AAGAAGCAGC GGTACGAGGT GGACTACACG
    CTGGAGCCCT TCGCGGGCCT CACCCCGGAG TACATGGAGA TGATCATCCA GTTCGGCTTC
    GTCACCCTGT TTGTCGCATC CTTCCCGCTG GCCCCACTCT TCGCGCTCCT GAACAACATC
    ATTGAGATCC GCCTGGACGC CAAAAAGTTT GTCACCGAGC TCCGAAGGCC GGTGGCTGTC
    AGAGCCAAGG ACATCGGAAT CTGGTACAAT ATCCTCAGAG GCGTCGGGAA GCTTGCCGTC
    ATCATCAACG CGTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC
    TACATGTACA GTGAGAACGG GACCATGCAT GGCTTCGTCA ACCACACCCT CTCTTCCTTC
    AACGTCAGCG ACTTCCAGAA CGGCACGGCC CCCAACGACC CCCTGGATCT GGGCTACGAG
    GTGCAGATCT GCAGGTACAA AGACTACCGG GAGCCGCCAT GGTCGGAACA CAAGTATGAC
    ATCTCCAAGG ACTTCTGGGC CGTCCTGGCC GCCCGGCTGG CCTTTGTCAT CGTCTTCCAG
    AACCTGGTCA TGTTCATGAG CGACTTTGTG GACTGGGTCA TCCCCGACAT CCCCAAGGAC
    ATCAGCCAGC AGATCCACAA AGAGAAGGTG CTCATGGTGG AGCTGTTCAT GCGGGAGGAG
    CAGGGCAAGC AGCAGCTGCT GGACACGTGG ATGGAGAAGG ACCGGAAGAA GGCGGAGCCC
    TGCAACAACC ACAGCCCCGC CCCAGCCGCC CCGCCCCTGC CCCCGCCCCC GGAAGGCCCC
    GACTTCCCCG GAGGCTCCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316846.1
    CDS68..3091
    Translation

    Target ORF information:

    RefSeq Version XM_015461360.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GAAGCGCCGG
    CACCTTCCGG AGGAGCCCAC AAACAAGTGG AGGCAGAGGG TTAAGACCGC CATGGCGGGG
    GTGAAATTGA CAGACAAGGT GAAGCTGACC TGGAGAGACC GGTTCCCTGC CTACTTCACC
    AATTTGGTGT CTATCATCTT CATGATCGCC GTGACCTTTG CCATTGTCCT TGGCGTCATC
    ATCTACAGAA TCTCCACGGC CGCCGCCTTG GCCATGAACT CCTCCCCGTC CGTGCGGTCC
    AACATCCGGG TCACGGTCAC AGCCACCGCG GTCATCATCA ACCTGGTGGT CATCATTCTC
    CTGGACGAGG TGTACGGCTG CATAGCCAGA TGGCTCACCA AGATAGAGGT TCCAAAGACG
    GAGAAGAGCT TTGAGGAGAG GCTGATCTTC AAGGCTTTCC TGCTCAAGTT CGTCAACTCC
    TACACACCCA TCTTCTACGT GGCTTTCTTC AAAGGCCGGT TTGTCGGACG CCCGGGTGAC
    TACGTGTACA TTTTCCGATC TTTCCGGATG GAGGAGTGCG CCCCGGGGGG CTGCCTGATG
    GAGCTCTGCA TCCAGCTCAG CATCATCATG CTGGGAAAGC AGCTGATCCA GAACAACCTG
    TTTGAGATCG GCATCCCGAA AATGAAGAAG TTCATCCGCT ATCTGAGGCT GAAGCACCAG
    AGCCCCTCGG ACCACGACGA GCACGTGAAG AAGAAGCAGC GGTACGAGGT GGACTACACG
    CTGGAGCCCT TCGCGGGCCT CACCCCGGAG TACATGGAGA TGATCATCCA GTTCGGCTTC
    GTCACCCTGT TTGTCGCATC CTTCCCGCTG GCCCCACTCT TCGCGCTCCT GAACAACATC
    ATTGAGATCC GCCTGGACGC CAAAAAGTTT GTCACCGAGC TCCGAAGGCC GGTGGCTGTC
    AGAGCCAAGG ACATCGGAAT CTGGTACAAT ATCCTCAGAG GCGTCGGGAA GCTTGCCGTC
    ATCATCAACG CGTTTGTGAT CTCCTTCACG TCTGACTTCA TCCCTCGCCT GGTGTACCTC
    TACATGTACA GTGAGAACGG GACCATGCAT GGCTTCGTCA ACCACACCCT CTCTTCCTTC
    AACGTCAGCG ACTTCCAGAA CGGCACGGCC CCCAACGACC CCCTGGATCT GGGCTACGAG
    GTGCAGATCT GCAGGTACAA AGACTACCGG GAGCCGCCAT GGTCGGAACA CAAGTATGAC
    ATCTCCAAGG ACTTCTGGGC CGTCCTGGCC GCCCGGCTGG CCTTTGTCAT CGTCTTCCAG
    AACCTGGTCA TGTTCATGAG CGACTTTGTG GACTGGGTCA TCCCCGACAT CCCCAAGGAC
    ATCAGCCAGC AGATCCACAA AGAGAAGGTG CTCATGGTGG AGCTGTTCAT GCGGGAGGAG
    CAGGGCAAGC AGCAGCTGCT GGACACGTGG ATGGAGAAGG ACCGGAAGAA GGCGGAGCCC
    TGCAACAACC ACAGCCCCGC CCCAGCCGCC CCGCCCCTGC CCCCGCCCCC GGAAGGCCCC
    GACTTCCCCG GAGGCTCCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89164
    Clone ID Related Accession (Same CDS sequence) XM_015461361.1
    Accession Version XM_015461361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GACAGACAAG
    GTGAAGCTGA CCTGGAGAGA CCGGTTCCCT GCCTACTTCA CCAATTTGGT GTCTATCATC
    TTCATGATCG CCGTGACCTT TGCCATTGTC CTTGGCGTCA TCATCTACAG AATCTCCACG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCG TCCGTGCGGT CCAACATCCG GGTCACGGTC
    ACAGCCACCG CGGTCATCAT CAACCTGGTG GTCATCATTC TCCTGGACGA GGTGTACGGC
    TGCATAGCCA GATGGCTCAC CAAGATAGAG GTTCCAAAGA CGGAGAAGAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTCAAG TTCGTCAACT CCTACACACC CATCTTCTAC
    GTGGCTTTCT TCAAAGGCCG GTTTGTCGGA CGCCCGGGTG ACTACGTGTA CATTTTCCGA
    TCTTTCCGGA TGGAGGAGTG CGCCCCGGGG GGCTGCCTGA TGGAGCTCTG CATCCAGCTC
    AGCATCATCA TGCTGGGAAA GCAGCTGATC CAGAACAACC TGTTTGAGAT CGGCATCCCG
    AAAATGAAGA AGTTCATCCG CTATCTGAGG CTGAAGCACC AGAGCCCCTC GGACCACGAC
    GAGCACGTGA AGAAGAAGCA GCGGTACGAG GTGGACTACA CGCTGGAGCC CTTCGCGGGC
    CTCACCCCGG AGTACATGGA GATGATCATC CAGTTCGGCT TCGTCACCCT GTTTGTCGCA
    TCCTTCCCGC TGGCCCCACT CTTCGCGCTC CTGAACAACA TCATTGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACCGA GCTCCGAAGG CCGGTGGCTG TCAGAGCCAA GGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCGTCGGG AAGCTTGCCG TCATCATCAA CGCGTTTGTG
    ATCTCCTTCA CGTCTGACTT CATCCCTCGC CTGGTGTACC TCTACATGTA CAGTGAGAAC
    GGGACCATGC ATGGCTTCGT CAACCACACC CTCTCTTCCT TCAACGTCAG CGACTTCCAG
    AACGGCACGG CCCCCAACGA CCCCCTGGAT CTGGGCTACG AGGTGCAGAT CTGCAGGTAC
    AAAGACTACC GGGAGCCGCC ATGGTCGGAA CACAAGTATG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CCGCCCGGCT GGCCTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCCGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAAGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAGGGCAA GCAGCAGCTG
    CTGGACACGT GGATGGAGAA GGACCGGAAG AAGGCGGAGC CCTGCAACAA CCACAGCCCC
    GCCCCAGCCG CCCCGCCCCT GCCCCCGCCC CCGGAAGGCC CCGACTTCCC CGGAGGCTCC
    CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316847.1
    CDS68..3013
    Translation

    Target ORF information:

    RefSeq Version XM_015461361.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG GCAAGGAGAG GGAAGAAAGA AGGACTCTGC CCTTTTAAAT
    AAGAGGCGCA AGTGTGATAG ATACGGCATC ACGAGCCTGC TGGCCAATGG TGTCTACTCG
    GCGGCCTACC CGCTGCATGA TGGAGACTAT GAAGGAGACA ACGTGGAGTT CAATGACAGG
    AAGCTCCTGT ACGAAGAGTG GGCGAGTTAC GGGGTCTTCT ACAAGTACCA GCCCATCGAC
    CTCGTCAGAA AGTATTTTGG GGAGAAGATT GGTCTGTACT TCGCCTGGCT TGGCGTCTAC
    ACCCAGATGC TCATCCCGGC CTCCGTGGTC GGCATCATCG TTTTCCTCTA CGGATGGGCC
    ACTGTCGATG ACAACATCCC CAGCATGGAG ATGTGCGACC AGAGGCACAA CATCACCATG
    TGCCCGCTCT GCGACAAGAC CTGCAGCTAC TGGAAGATGA GCTCCGCGTG CGCCACCGCC
    CGCGCCAGCC ACCTCTTCGA CAACCCCGCC ACTGTCTTCT TCTCCATCTT CATGGCCCTC
    TGGGCTGCCA CCTTCATGGA GCACTGGAAG CGGAAACAGA TGCGGCTCAA TTACCGGTGG
    GACCTCACAG GCTTCGAGGA GGAGGAGGAG GCTGTCAAGG ACCATCCCAG AGCTGAATAT
    GAAGCCCGAG TTCTGGAGAA GTCTCTTAGG AAAGAATCCA AAAACAAAGA GACAGACAAG
    GTGAAGCTGA CCTGGAGAGA CCGGTTCCCT GCCTACTTCA CCAATTTGGT GTCTATCATC
    TTCATGATCG CCGTGACCTT TGCCATTGTC CTTGGCGTCA TCATCTACAG AATCTCCACG
    GCCGCCGCCT TGGCCATGAA CTCCTCCCCG TCCGTGCGGT CCAACATCCG GGTCACGGTC
    ACAGCCACCG CGGTCATCAT CAACCTGGTG GTCATCATTC TCCTGGACGA GGTGTACGGC
    TGCATAGCCA GATGGCTCAC CAAGATAGAG GTTCCAAAGA CGGAGAAGAG CTTTGAGGAG
    AGGCTGATCT TCAAGGCTTT CCTGCTCAAG TTCGTCAACT CCTACACACC CATCTTCTAC
    GTGGCTTTCT TCAAAGGCCG GTTTGTCGGA CGCCCGGGTG ACTACGTGTA CATTTTCCGA
    TCTTTCCGGA TGGAGGAGTG CGCCCCGGGG GGCTGCCTGA TGGAGCTCTG CATCCAGCTC
    AGCATCATCA TGCTGGGAAA GCAGCTGATC CAGAACAACC TGTTTGAGAT CGGCATCCCG
    AAAATGAAGA AGTTCATCCG CTATCTGAGG CTGAAGCACC AGAGCCCCTC GGACCACGAC
    GAGCACGTGA AGAAGAAGCA GCGGTACGAG GTGGACTACA CGCTGGAGCC CTTCGCGGGC
    CTCACCCCGG AGTACATGGA GATGATCATC CAGTTCGGCT TCGTCACCCT GTTTGTCGCA
    TCCTTCCCGC TGGCCCCACT CTTCGCGCTC CTGAACAACA TCATTGAGAT CCGCCTGGAC
    GCCAAAAAGT TTGTCACCGA GCTCCGAAGG CCGGTGGCTG TCAGAGCCAA GGACATCGGA
    ATCTGGTACA ATATCCTCAG AGGCGTCGGG AAGCTTGCCG TCATCATCAA CGCGTTTGTG
    ATCTCCTTCA CGTCTGACTT CATCCCTCGC CTGGTGTACC TCTACATGTA CAGTGAGAAC
    GGGACCATGC ATGGCTTCGT CAACCACACC CTCTCTTCCT TCAACGTCAG CGACTTCCAG
    AACGGCACGG CCCCCAACGA CCCCCTGGAT CTGGGCTACG AGGTGCAGAT CTGCAGGTAC
    AAAGACTACC GGGAGCCGCC ATGGTCGGAA CACAAGTATG ACATCTCCAA GGACTTCTGG
    GCCGTCCTGG CCGCCCGGCT GGCCTTTGTC ATCGTCTTCC AGAACCTGGT CATGTTCATG
    AGCGACTTTG TGGACTGGGT CATCCCCGAC ATCCCCAAGG ACATCAGCCA GCAGATCCAC
    AAAGAGAAGG TGCTCATGGT GGAGCTGTTC ATGCGGGAGG AGCAGGGCAA GCAGCAGCTG
    CTGGACACGT GGATGGAGAA GGACCGGAAG AAGGCGGAGC CCTGCAACAA CCACAGCCCC
    GCCCCAGCCG CCCCGCCCCT GCCCCCGCCC CCGGAAGGCC CCGACTTCCC CGGAGGCTCC
    CTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89165
    Clone ID Related Accession (Same CDS sequence) XM_015461362.1
    Accession Version XM_015461362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2880bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product anoctamin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTCTA CTCGGCGGCC
    TACCCGCTGC ATGATGGAGA CTATGAAGGA GACAACGTGG AGTTCAATGA CAGGAAGCTC
    CTGTACGAAG AGTGGGCGAG TTACGGGGTC TTCTACAAGT ACCAGCCCAT CGACCTCGTC
    AGAAAGTATT TTGGGGAGAA GATTGGTCTG TACTTCGCCT GGCTTGGCGT CTACACCCAG
    ATGCTCATCC CGGCCTCCGT GGTCGGCATC ATCGTTTTCC TCTACGGATG GGCCACTGTC
    GATGACAACA TCCCCAGCAT GGAGATGTGC GACCAGAGGC ACAACATCAC CATGTGCCCG
    CTCTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCCG CGTGCGCCAC CGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACTGTC TTCTTCTCCA TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGGC TCAATTACCG GTGGGACCTC
    ACAGGCTTCG AGGAGGAGGA GGAGGCTGTC AAGGACCATC CCAGAGCTGA ATATGAAGCC
    CGAGTTCTGG AGAAGTCTCT TAGGAAAGAA TCCAAAAACA AAGAGACAGA CAAGGTGAAG
    CTGACCTGGA GAGACCGGTT CCCTGCCTAC TTCACCAATT TGGTGTCTAT CATCTTCATG
    ATCGCCGTGA CCTTTGCCAT TGTCCTTGGC GTCATCATCT ACAGAATCTC CACGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCGTCCGTG CGGTCCAACA TCCGGGTCAC GGTCACAGCC
    ACCGCGGTCA TCATCAACCT GGTGGTCATC ATTCTCCTGG ACGAGGTGTA CGGCTGCATA
    GCCAGATGGC TCACCAAGAT AGAGGTTCCA AAGACGGAGA AGAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT CAAGTTCGTC AACTCCTACA CACCCATCTT CTACGTGGCT
    TTCTTCAAAG GCCGGTTTGT CGGACGCCCG GGTGACTACG TGTACATTTT CCGATCTTTC
    CGGATGGAGG AGTGCGCCCC GGGGGGCTGC CTGATGGAGC TCTGCATCCA GCTCAGCATC
    ATCATGCTGG GAAAGCAGCT GATCCAGAAC AACCTGTTTG AGATCGGCAT CCCGAAAATG
    AAGAAGTTCA TCCGCTATCT GAGGCTGAAG CACCAGAGCC CCTCGGACCA CGACGAGCAC
    GTGAAGAAGA AGCAGCGGTA CGAGGTGGAC TACACGCTGG AGCCCTTCGC GGGCCTCACC
    CCGGAGTACA TGGAGATGAT CATCCAGTTC GGCTTCGTCA CCCTGTTTGT CGCATCCTTC
    CCGCTGGCCC CACTCTTCGC GCTCCTGAAC AACATCATTG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CCGAGCTCCG AAGGCCGGTG GCTGTCAGAG CCAAGGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCGT CGGGAAGCTT GCCGTCATCA TCAACGCGTT TGTGATCTCC
    TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTGA GAACGGGACC
    ATGCATGGCT TCGTCAACCA CACCCTCTCT TCCTTCAACG TCAGCGACTT CCAGAACGGC
    ACGGCCCCCA ACGACCCCCT GGATCTGGGC TACGAGGTGC AGATCTGCAG GTACAAAGAC
    TACCGGGAGC CGCCATGGTC GGAACACAAG TATGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCCGCCC GGCTGGCCTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC CGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAAGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAGG GCAAGCAGCA GCTGCTGGAC
    ACGTGGATGG AGAAGGACCG GAAGAAGGCG GAGCCCTGCA ACAACCACAG CCCCGCCCCA
    GCCGCCCCGC CCCTGCCCCC GCCCCCGGAA GGCCCCGACT TCCCCGGAGG CTCCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316848.1
    CDS68..2947
    Translation

    Target ORF information:

    RefSeq Version XM_015461362.1
    Organism Bos taurus(cattle)
    Definition Bos taurus anoctamin 1, calcium activated chloride channel (ANO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGGTCA ACGAGAAGTA CTCGACGCTC CCGGCCGAGG ACCGCAGCGT CCACATCATC 
    AACATCTGCG CCATCGAGGA CATCGGCTAC CTGCCGTCCG AGGGCACGCT GCTGAACTCC
    TTGTCCGTGG ACCCGGACGC CGAGTGCAAG TACGGCCTGT ACTTCCGCGA CGGCAAGCGC
    AAGGTGGACT ATGTCCTGGT GTACCATCAC AAGAGGGCCT CGGGCGGCAG GACGCTGGCC
    AGGAGGGCCC AGCACGGCGA CGCGGGCCCG GCCGCCCGCA GCGCCCGGCA GGACCAGCCC
    CTGCCCGGGA AGGCGGGCCC CCTGGGCGCG GGCGAGCCCG AGCCGCCGCT GGACTACCAC
    GAAGATGACA AGCGCTTCCG CCGGGAGGAG TACGAGGGCA ACCTGGTGGA GGCCGGCCTG
    GAGCTGGAGC GGGACGAGGA CACAAAAATT CACGGGGTTG GATTTGTAAA AATCCACGCA
    CCCTGGAATG TGCTGTGCCG AGAAGCCGAG TTTTTGAAGC TGAAGATGCC GACAAAGAAG
    CTGTATCACA TCAGCGAGAC CCGCGGCCTC CTCAAGAAGA TCAACTCTGT GCTCCAGAAG
    ATCACGGACC CCATCCAGCC CAAGGTGGCA GAGCACAGGC CCCAGACCAC CAAGAGGCTC
    TCCTACCCCT TTTCCCGGGA GAAGCAGCAC CTATTTGACC TGTCTGACAA GGATTCCTTT
    TTCGACAGCA AAACTCGGAG CACAATAGTC TACGAAATCC TGAAGAGAAC AACGTGTACG
    AAGGCCAAGT ACAGCATGGG CATCACGAGC CTGCTGGCCA ATGGTGTCTA CTCGGCGGCC
    TACCCGCTGC ATGATGGAGA CTATGAAGGA GACAACGTGG AGTTCAATGA CAGGAAGCTC
    CTGTACGAAG AGTGGGCGAG TTACGGGGTC TTCTACAAGT ACCAGCCCAT CGACCTCGTC
    AGAAAGTATT TTGGGGAGAA GATTGGTCTG TACTTCGCCT GGCTTGGCGT CTACACCCAG
    ATGCTCATCC CGGCCTCCGT GGTCGGCATC ATCGTTTTCC TCTACGGATG GGCCACTGTC
    GATGACAACA TCCCCAGCAT GGAGATGTGC GACCAGAGGC ACAACATCAC CATGTGCCCG
    CTCTGCGACA AGACCTGCAG CTACTGGAAG ATGAGCTCCG CGTGCGCCAC CGCCCGCGCC
    AGCCACCTCT TCGACAACCC CGCCACTGTC TTCTTCTCCA TCTTCATGGC CCTCTGGGCT
    GCCACCTTCA TGGAGCACTG GAAGCGGAAA CAGATGCGGC TCAATTACCG GTGGGACCTC
    ACAGGCTTCG AGGAGGAGGA GGAGGCTGTC AAGGACCATC CCAGAGCTGA ATATGAAGCC
    CGAGTTCTGG AGAAGTCTCT TAGGAAAGAA TCCAAAAACA AAGAGACAGA CAAGGTGAAG
    CTGACCTGGA GAGACCGGTT CCCTGCCTAC TTCACCAATT TGGTGTCTAT CATCTTCATG
    ATCGCCGTGA CCTTTGCCAT TGTCCTTGGC GTCATCATCT ACAGAATCTC CACGGCCGCC
    GCCTTGGCCA TGAACTCCTC CCCGTCCGTG CGGTCCAACA TCCGGGTCAC GGTCACAGCC
    ACCGCGGTCA TCATCAACCT GGTGGTCATC ATTCTCCTGG ACGAGGTGTA CGGCTGCATA
    GCCAGATGGC TCACCAAGAT AGAGGTTCCA AAGACGGAGA AGAGCTTTGA GGAGAGGCTG
    ATCTTCAAGG CTTTCCTGCT CAAGTTCGTC AACTCCTACA CACCCATCTT CTACGTGGCT
    TTCTTCAAAG GCCGGTTTGT CGGACGCCCG GGTGACTACG TGTACATTTT CCGATCTTTC
    CGGATGGAGG AGTGCGCCCC GGGGGGCTGC CTGATGGAGC TCTGCATCCA GCTCAGCATC
    ATCATGCTGG GAAAGCAGCT GATCCAGAAC AACCTGTTTG AGATCGGCAT CCCGAAAATG
    AAGAAGTTCA TCCGCTATCT GAGGCTGAAG CACCAGAGCC CCTCGGACCA CGACGAGCAC
    GTGAAGAAGA AGCAGCGGTA CGAGGTGGAC TACACGCTGG AGCCCTTCGC GGGCCTCACC
    CCGGAGTACA TGGAGATGAT CATCCAGTTC GGCTTCGTCA CCCTGTTTGT CGCATCCTTC
    CCGCTGGCCC CACTCTTCGC GCTCCTGAAC AACATCATTG AGATCCGCCT GGACGCCAAA
    AAGTTTGTCA CCGAGCTCCG AAGGCCGGTG GCTGTCAGAG CCAAGGACAT CGGAATCTGG
    TACAATATCC TCAGAGGCGT CGGGAAGCTT GCCGTCATCA TCAACGCGTT TGTGATCTCC
    TTCACGTCTG ACTTCATCCC TCGCCTGGTG TACCTCTACA TGTACAGTGA GAACGGGACC
    ATGCATGGCT TCGTCAACCA CACCCTCTCT TCCTTCAACG TCAGCGACTT CCAGAACGGC
    ACGGCCCCCA ACGACCCCCT GGATCTGGGC TACGAGGTGC AGATCTGCAG GTACAAAGAC
    TACCGGGAGC CGCCATGGTC GGAACACAAG TATGACATCT CCAAGGACTT CTGGGCCGTC
    CTGGCCGCCC GGCTGGCCTT TGTCATCGTC TTCCAGAACC TGGTCATGTT CATGAGCGAC
    TTTGTGGACT GGGTCATCCC CGACATCCCC AAGGACATCA GCCAGCAGAT CCACAAAGAG
    AAGGTGCTCA TGGTGGAGCT GTTCATGCGG GAGGAGCAGG GCAAGCAGCA GCTGCTGGAC
    ACGTGGATGG AGAAGGACCG GAAGAAGGCG GAGCCCTGCA ACAACCACAG CCCCGCCCCA
    GCCGCCCCGC CCCTGCCCCC GCCCCCGGAA GGCCCCGACT TCCCCGGAGG CTCCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.