GC cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GC
Entrez Gene ID	530076
Full Name	GC, vitamin D binding protein
General protein information	Preferred Names GC, vitamin D binding protein Names vitamin D-binding protein DBP VDB gc-globulin group-specific component (vitamin D binding protein)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005208092.2	XP_005208149.1	vitamin D-binding protein isoform X1
XM_005208092.3	XP_005208149.1	vitamin D-binding protein isoform X1
NM_001035380.2	NP_001030457.1	vitamin D-binding protein precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GC	NP_990213.1
Homo sapiens (human)	GC	NP_001191236.1
	GC	XP_001104833.2
Rattus norvegicus (Norway rat)	Gc	NP_036696.2
Danio rerio (zebrafish)	gc	NP_001002568.1
Xenopus tropicalis (tropical clawed frog)	gc	NP_001015745.1
Pan troglodytes (chimpanzee)	GC	XP_517242.3
Canis lupus familiaris (dog)	GC	XP_005628336.1
Bos taurus (cattle)	GC	NP_001030457.1
Mus musculus (house mouse)	Gc	NP_032122.1

Gene Ontology
Bibliography

Related articles in PubMed

Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production.
Olsen HG, Knutsen TM, Lewandowska-Sabat AM, Grove H, Nome T, Svendsen M, Arnyasi M, Sodeland M, Sundsaasen KK, Dahl SR, Heringstad B, Hansen HH, Olsaker I, Kent MP, Lien S
Genetics, selection, evolution : GSE48(1)79(2016 10)

Pregnancy-associated changes in genome-wide gene expression profiles in the liver of cow throughout pregnancy.
Herath CB, Shiojima S, Ishiwata H, Katsuma S, Kadowaki T, Ushizawa K, Imai K, Takahashi T, Hirasawa A, Tsujimoto G, Hashizume K
Biochemical and biophysical research communications313(3)666-80(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production.
Title: Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production.

The following GC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00242	NM_001035380.2 Latest version!	Bos taurus GC vitamin D binding protein (GC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa20522	XM_005208092.2 Latest version!	Bos taurus group-specific component (vitamin D binding protein) (GC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa235330	XM_005208092.3 Latest version!	Bos taurus GC, vitamin D binding protein (GC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GC ( NM_001035380.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001030457.1 Bos taurus GC vitamin D binding protein (GC), mRNA.

Price & Availability↑

CloneID	OBa00242
Clone ID Related Accession (Same CDS sequence)	NM_001035380.2
Accession Version	NM_001035380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Bos taurus(cattle)
Product	vitamin D-binding protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105171.1. On Aug 31, 2012 this sequence version replaced NM_001035380.1. ##Evidence-Data-START## Transcript exon combination :: BC105171.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGAAGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAATGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GACCCTAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGACGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC AGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGCTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030457.1
CDS	65..1489
Translation	MKRILVFLLAVAFVHALERGRDYEKDKVCKDLASLGREDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTVTCCAEGADPDCYDNRTSALSDKSCESNSPFPVHPGTPECCTHEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFADRFMYEYSINYGQAPLTLLVGYTKSYLSMVGSCCTSPNPTVCFLKERLQLKHFSLLTIMTNRICSQYAAYGKEKSRLSHLIKFAQKVPTAHLEDVLPLAEDITTILSKCCDSVSEDCIKELPEYAVKLCDNLSTKNSKFKDCCQEKTPMEIFVCAYFMPASPNPELPDVKLPMNKDVCDEGNTKVLDQYIFELSRKTQIPEVFLTKILESTLKSLDECCHSESSTACLNAKGPQLTRELSSFIQKGQELCADYSENTFTEYKKKLAERLRGKFPDATETDLQELVAKRSDFASKCCSVNSPPLYCNSEIDAEINTLQS

Target ORF information:

RefSeq Version	NM_001035380.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GC vitamin D binding protein (GC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035380.2

ORF Insert Sequence:

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGAAGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAATGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GACCCTAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGACGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC AGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGCTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GC ( XM_005208092.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208149.1 Bos taurus group-specific component (vitamin D binding protein) (GC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20522
Clone ID Related Accession (Same CDS sequence)	XM_005208092.2
Accession Version	XM_005208092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	vitamin D-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005208092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGATGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAACGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GATCCCAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGATGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC GGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGTTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208149.1
CDS	177..1601
Translation	MKRILVFLLAVAFVHALERGRDYEKDKVCKDLASLGRDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTVTCCAEGADPDCYDNRTSALSDKSCESNSPFPVHPGTPECCTHEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFADRFMYEYSINYGQAPLTLLVGYTKSYLSMVGSCCTSPNPTVCFLKERLQLKHFSLLTIMTNRICSQYAAYGKEKSRLSHLIKFAQKVPTAHLEDVLPLAEDITTILSKCCDSVSEDCIKELPEYAVKLCDNLSTKNSKFKDCCQEKTPMEIFVCAYFMPASPNPELPDVKLPMNKDVCDEGNTKVLDQYIFELSRKTRIPEVFLTKILESTLKSLDECCHSESSTACLNAKGPQLTRELSSFIQKGQELCADYSENTFTEYKKKLAERLRGKFPDATETDLQELVAKRSDFASKCCSVNSPPLYCNSEIDAEINTLQS

Target ORF information:

RefSeq Version	XM_005208092.2
Organism	Bos taurus(cattle)
Definition	Bos taurus group-specific component (vitamin D binding protein) (GC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208092.2

ORF Insert Sequence:

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGATGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAACGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GATCCCAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGATGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC GGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGTTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GC ( XM_005208092.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208149.1 Bos taurus GC, vitamin D binding protein (GC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235330
Clone ID Related Accession (Same CDS sequence)	XM_005208092.3
Accession Version	XM_005208092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	vitamin D-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGATGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAATGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GATCCCAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGATGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC GGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGTTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208149.1
CDS	177..1601
Translation	MKRILVFLLAVAFVHALERGRDYEKDKVCKDLASLGRDDFTSLSMVLYSRKFPSGTFEQISHLVNEVVSLTVTCCAEGADPDCYDNRTSALSDKSCESNSPFPVHPGTPECCTHEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFADRFMYEYSINYGQAPLTLLVGYTKSYLSMVGSCCTSPNPTVCFLKERLQLKHFSLLTIMTNRICSQYAAYGKEKSRLSHLIKFAQKVPTAHLEDVLPLAEDITTILSKCCDSVSEDCIKELPEYAVKLCDNLSTKNSKFKDCCQEKTPMEIFVCAYFMPASPNPELPDVKLPMNKDVCDEGNTKVLDQYIFELSRKTRIPEVFLTKILESTLKSLDECCHSESSTACLNAKGPQLTRELSSFIQKGQELCADYSENTFTEYKKKLAERLRGKFPDATETDLQELVAKRSDFASKCCSVNSPPLYCNSEIDAEINTLQS

Target ORF information:

RefSeq Version	XM_005208092.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GC, vitamin D binding protein (GC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208092.3

ORF Insert Sequence:

ATGAAGAGGA TCCTGGTTTT CCTCCTTGCT GTAGCCTTTG TGCATGCTCT AGAGAGAGGC 
CGAGATTATG AGAAAGATAA AGTCTGCAAG GACCTTGCCA GCCTGGGAAG AGATGACTTC 
ACATCTTTGT CAATGGTCCT ATACAGCAGA AAATTTCCCA GTGGCACCTT TGAACAGATC 
AGCCATCTTG TGAATGAAGT TGTTTCCTTA ACAGTAACCT GCTGTGCGGA AGGGGCCGAT 
CCTGACTGCT ATGACAACAG GACTTCAGCA TTGTCTGACA AGTCCTGTGA AAGCAACTCT 
CCATTCCCAG TGCACCCTGG AACTCCTGAG TGCTGCACCC ATGAGGGCCT TGAAAAGAAG 
CTCTGTATGG CTGCCCTGAA GCACCAGCCA CAAGAATTTC CAACTTACGT AGAGCCAACA 
AATGACGAGA TCTGTGAGGC ATTCAGGAAA GATCCCAAGG ACTTTGCTGA TCGATTTATG 
TATGAATATT CCATTAATTA TGGACAAGCT CCTCTGACAC TTTTAGTCGG TTACACCAAG 
AGTTATCTCT CTATGGTAGG GTCCTGCTGT ACTTCACCAA ACCCAACAGT ATGCTTTTTG 
AAAGAGAGAC TTCAGCTTAA ACATTTTTCA CTTCTCACCA TTATGACAAA CAGAATCTGC 
TCACAATACG CTGCTTATGG GAAGGAGAAG TCAAGGCTCA GCCATCTCAT CAAATTTGCC 
CAGAAAGTGC CTACTGCACA TCTGGAAGAT GTTCTGCCAC TTGCTGAAGA TATTACAACA 
ATCCTCTCCA AGTGCTGTGA CTCTGTCTCT GAGGACTGCA TCAAGGAGCT TCCTGAATAT 
GCAGTAAAAC TCTGTGACAA CTTATCCACT AAAAATTCGA AATTTAAGGA CTGTTGTCAA 
GAAAAAACAC CCATGGAGAT ATTTGTGTGC GCTTACTTCA TGCCAGCTTC CCCAAACCCT 
GAATTGCCAG ATGTCAAGTT GCCCATGAAC AAAGATGTGT GTGATGAAGG AAACACCAAG 
GTCCTGGATC AGTATATATT TGAACTAAGC AGGAAGACTC GGATTCCAGA AGTATTCCTC 
ACTAAAATAC TTGAGTCAAC CCTGAAAAGC CTTGATGAAT GCTGCCACTC TGAATCTTCT 
ACTGCCTGTT TAAATGCAAA GGGCCCTCAA TTGACAAGGG AATTATCATC TTTCATTCAA 
AAAGGACAAG AACTATGTGC TGATTATTCA GAAAACACAT TTACTGAGTA CAAGAAAAAA 
CTGGCAGAGC GACTAAGAGG AAAATTTCCT GATGCCACGG AGACGGATCT CCAAGAGCTG 
GTTGCCAAGC GCTCAGACTT TGCCTCCAAG TGTTGTTCTG TAAACTCACC TCCTCTCTAC 
TGCAATTCAG AGATTGATGC TGAAATTAAT ACCCTACAGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production.
Genetics, selection, evolution : GSE48(1)79(2016 10)
Olsen HG,Knutsen TM,Lewandowska-Sabat AM,Grove H,Nome T,Svendsen M,Arnyasi M,Sodeland M,Sundsaasen KK,Dahl SR,Heringstad B,Hansen HH,Olsaker I,Kent MP,Lien S

Pregnancy-associated changes in genome-wide gene expression profiles in the liver of cow throughout pregnancy.
Biochemical and biophysical research communications313(3)666-80(2004 Jan)
Herath CB,Shiojima S,Ishiwata H,Katsuma S,Kadowaki T,Ushizawa K,Imai K,Takahashi T,Hirasawa A,Tsujimoto G,Hashizume K

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GC cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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