CBLB cDNA ORF clone, Bos taurus(cattle)

The following CBLB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CBLB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa15620 NM_001205923.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15620 XM_005201316.3
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15620 XM_005201317.3
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15620 XM_010801101.2
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15620 XM_010801102.2
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa15620 XM_010801103.2
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa51098 XM_010801104.2
Latest version!
Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa218773 NM_001205923.2
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218773 XM_005201316.4
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218773 XM_005201317.4
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218773 XM_010801102.3
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218773 XM_010801101.3
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218774 XM_010801104.3
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218775 XM_024989731.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218775 XM_024989740.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218776 XM_024989734.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218777 XM_024989738.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa218778 XM_024989742.1
Latest version!
Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version NM_001205923.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02001195.1, DAAA02001193.1 and DAAA02001192.1. On Apr 20, 2011 this sequence version replaced XM_604265.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403654 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192852.1
    CDS1..2952
    Translation

    Target ORF information:

    RefSeq Version NM_001205923.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205923.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version XM_010801101.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799403.1
    CDS155..3106
    Translation

    Target ORF information:

    RefSeq Version XM_010801101.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801101.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version XM_005201316.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201373.1
    CDS151..3102
    Translation

    Target ORF information:

    RefSeq Version XM_005201316.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201316.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version XM_010801102.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799404.1
    CDS36..2987
    Translation

    Target ORF information:

    RefSeq Version XM_010801102.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801102.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version XM_005201317.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201374.1
    CDS179..3130
    Translation

    Target ORF information:

    RefSeq Version XM_005201317.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201317.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15620
    Clone ID Related Accession (Same CDS sequence) XM_010801101.2 , XM_005201316.3 , XM_010801102.2 , NM_001205923.1 , XM_005201317.3 , XM_010801103.2
    Accession Version XM_010801103.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799405.1
    CDS596..3547
    Translation

    Target ORF information:

    RefSeq Version XM_010801103.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801103.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa51098
    Clone ID Related Accession (Same CDS sequence) XM_010801104.2
    Accession Version XM_010801104.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103810.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG CGAAGATGCT TTTGACGCTC TGCCTCCGTC CCTCCCACCT
    CCCCCACCTC CCGCAAGGCA CAGTCTCATC GAACATTCGA AACCTCCTGG CTCTGGCAGC
    CGACCATCCT CAGGACAGGA CCTTTTCCTC CTTCCTTCAG ATCCCTTTTT TGATCCGGCA
    AGTGGCCAAG TTCCTTTGCC TCCTGCTAGG AGATTACCAG GTGAAAATGT CAAATCCAAC
    AGAGCATCAC AGGACTATGA TCCGCTTCCT TCATCTTCAG ATGGTTCACA AGCACCAGCC
    AGACCTCCTA AACCACGACC CCGCAGGACT GCACCAGAAA TTCACCACAG AAAACCCCAC
    GGGCCTGAGG CAGCATTGGA AAATGTCGAT GCAAAAATTG CAAAACTCAT GGGAGAGGGT
    TATGCCTTTG AAGAAGTGAA AAGAGCCTTA GAGATAGCCC AGAATAACGT GGAAGTGGCC
    CGTAGCATCC TTCGGGAATT TGCCTTCCCC CCTCCAGTCT CCCCACGTCT AAATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799406.1
    CDS151..2970
    Translation

    Target ORF information:

    RefSeq Version XM_010801104.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B, E3 ubiquitin protein ligase (CBLB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801104.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAT GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG CGAAGATGCT TTTGACGCTC TGCCTCCGTC CCTCCCACCT
    CCCCCACCTC CCGCAAGGCA CAGTCTCATC GAACATTCGA AACCTCCTGG CTCTGGCAGC
    CGACCATCCT CAGGACAGGA CCTTTTCCTC CTTCCTTCAG ATCCCTTTTT TGATCCGGCA
    AGTGGCCAAG TTCCTTTGCC TCCTGCTAGG AGATTACCAG GTGAAAATGT CAAATCCAAC
    AGAGCATCAC AGGACTATGA TCCGCTTCCT TCATCTTCAG ATGGTTCACA AGCACCAGCC
    AGACCTCCTA AACCACGACC CCGCAGGACT GCACCAGAAA TTCACCACAG AAAACCCCAC
    GGGCCTGAGG CAGCATTGGA AAATGTCGAT GCAAAAATTG CAAAACTCAT GGGAGAGGGT
    TATGCCTTTG AAGAAGTGAA AAGAGCCTTA GAGATAGCCC AGAATAACGT GGAAGTGGCC
    CGTAGCATCC TTCGGGAATT TGCCTTCCCC CCTCCAGTCT CCCCACGTCT AAATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218773
    Clone ID Related Accession (Same CDS sequence) XM_005201317.4 , XM_010801101.3 , XM_005201316.4 , XM_010801102.3 , NM_001205923.2
    Accession Version NM_001205923.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On May 30, 2018 this sequence version replaced NM_001205923.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721694.5936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192852.1
    CDS1..2952
    Translation

    Target ORF information:

    RefSeq Version NM_001205923.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205923.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218773
    Clone ID Related Accession (Same CDS sequence) XM_005201317.4 , XM_010801101.3 , XM_005201316.4 , XM_010801102.3 , NM_001205923.2
    Accession Version XM_005201317.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201374.1
    CDS236..3187
    Translation

    Target ORF information:

    RefSeq Version XM_005201317.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201317.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218773
    Clone ID Related Accession (Same CDS sequence) XM_005201317.4 , XM_010801101.3 , XM_005201316.4 , XM_010801102.3 , NM_001205923.2
    Accession Version XM_010801101.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799403.1
    CDS154..3105
    Translation

    Target ORF information:

    RefSeq Version XM_010801101.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801101.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218773
    Clone ID Related Accession (Same CDS sequence) XM_005201317.4 , XM_010801101.3 , XM_005201316.4 , XM_010801102.3 , NM_001205923.2
    Accession Version XM_005201316.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201373.1
    CDS174..3125
    Translation

    Target ORF information:

    RefSeq Version XM_005201316.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201316.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218773
    Clone ID Related Accession (Same CDS sequence) XM_005201317.4 , XM_010801101.3 , XM_005201316.4 , XM_010801102.3 , NM_001205923.2
    Accession Version XM_010801102.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799404.1
    CDS210..3161
    Translation

    Target ORF information:

    RefSeq Version XM_010801102.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801102.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG AGATGTTTTT GATTCAGCCT CTGATCCAGT GCCATTGCCA
    CCTGCCAGGC CTCCAACTCG GGACAATCCA AAGCATGGTT CTTCACTCAA CAGGACGCCC
    TCTGATTATG ATCTTCTCAT CCCTCCATTA GGCGAAGATG CTTTTGACGC TCTGCCTCCG
    TCCCTCCCAC CTCCCCCACC TCCCGCAAGG CACAGTCTCA TCGAACATTC GAAACCTCCT
    GGCTCTGGCA GCCGACCATC CTCAGGACAG GACCTTTTCC TCCTTCCTTC AGATCCCTTT
    TTTGATCCGG CAAGTGGCCA AGTTCCTTTG CCTCCTGCTA GGAGATTACC AGGTGAAAAT
    GTCAAATCCA ACAGAGCATC ACAGGACTAT GATCCGCTTC CTTCATCTTC AGATGGTTCA
    CAAGCACCAG CCAGACCTCC TAAACCACGA CCCCGCAGGA CTGCACCAGA AATTCACCAC
    AGAAAACCCC ACGGGCCTGA GGCAGCATTG GAAAATGTCG ATGCAAAAAT TGCAAAACTC
    ATGGGAGAGG GTTATGCCTT TGAAGAAGTG AAAAGAGCCT TAGAGATAGC CCAGAATAAC
    GTGGAAGTGG CCCGTAGCAT CCTTCGGGAA TTTGCCTTCC CCCCTCCAGT CTCCCCACGT
    CTAAATCTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218774
    Clone ID Related Accession (Same CDS sequence) XM_010801104.3
    Accession Version XM_010801104.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801104.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG CGAAGATGCT TTTGACGCTC TGCCTCCGTC CCTCCCACCT
    CCCCCACCTC CCGCAAGGCA CAGTCTCATC GAACATTCGA AACCTCCTGG CTCTGGCAGC
    CGACCATCCT CAGGACAGGA CCTTTTCCTC CTTCCTTCAG ATCCCTTTTT TGATCCGGCA
    AGTGGCCAAG TTCCTTTGCC TCCTGCTAGG AGATTACCAG GTGAAAATGT CAAATCCAAC
    AGAGCATCAC AGGACTATGA TCCGCTTCCT TCATCTTCAG ATGGTTCACA AGCACCAGCC
    AGACCTCCTA AACCACGACC CCGCAGGACT GCACCAGAAA TTCACCACAG AAAACCCCAC
    GGGCCTGAGG CAGCATTGGA AAATGTCGAT GCAAAAATTG CAAAACTCAT GGGAGAGGGT
    TATGCCTTTG AAGAAGTGAA AAGAGCCTTA GAGATAGCCC AGAATAACGT GGAAGTGGCC
    CGTAGCATCC TTCGGGAATT TGCCTTCCCC CCTCCAGTCT CCCCACGTCT AAATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799406.1
    CDS174..2993
    Translation

    Target ORF information:

    RefSeq Version XM_010801104.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801104.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAGTGTAC TGGTCCACTA GCGAATTCCC TTTCAGAGAA AACAAGAGAC
    CCGGTAGAGG AAGATGACGA TGAATACAAG GTTCCTTCAT CCCATCCTGT TTCCTTGAGT
    TCACAACCAT CTCATTGCCA TAATGTAAAA CCTCCTCCTA GATCTTGTGA TAACGGTCAC
    TGTATATTGA ATGGAACACA TGGCACATCC TCAGAGATGA AGAAATCAAA CATCCCTGAG
    TTAGGCATAT ATTTAAAGGG CGAAGATGCT TTTGACGCTC TGCCTCCGTC CCTCCCACCT
    CCCCCACCTC CCGCAAGGCA CAGTCTCATC GAACATTCGA AACCTCCTGG CTCTGGCAGC
    CGACCATCCT CAGGACAGGA CCTTTTCCTC CTTCCTTCAG ATCCCTTTTT TGATCCGGCA
    AGTGGCCAAG TTCCTTTGCC TCCTGCTAGG AGATTACCAG GTGAAAATGT CAAATCCAAC
    AGAGCATCAC AGGACTATGA TCCGCTTCCT TCATCTTCAG ATGGTTCACA AGCACCAGCC
    AGACCTCCTA AACCACGACC CCGCAGGACT GCACCAGAAA TTCACCACAG AAAACCCCAC
    GGGCCTGAGG CAGCATTGGA AAATGTCGAT GCAAAAATTG CAAAACTCAT GGGAGAGGGT
    TATGCCTTTG AAGAAGTGAA AAGAGCCTTA GAGATAGCCC AGAATAACGT GGAAGTGGCC
    CGTAGCATCC TTCGGGAATT TGCCTTCCCC CCTCCAGTCT CCCCACGTCT AAATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218775
    Clone ID Related Accession (Same CDS sequence) XM_024989740.1 , XM_024989731.1
    Accession Version XM_024989740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGAGATGTT
    TTTGATTCAG CCTCTGATCC AGTGCCATTG CCACCTGCCA GGCCTCCAAC TCGGGACAAT
    CCAAAGCATG GTTCTTCACT CAACAGGACG CCCTCTGATT ATGATCTTCT CATCCCTCCA
    TTAGGCGAAG ATGCTTTTGA CGCTCTGCCT CCGTCCCTCC CACCTCCCCC ACCTCCCGCA
    AGGCACAGTC TCATCGAACA TTCGAAACCT CCTGGCTCTG GCAGCCGACC ATCCTCAGGA
    CAGGACCTTT TCCTCCTTCC TTCAGATCCC TTTTTTGATC CGGCAAGTGG CCAAGTTCCT
    TTGCCTCCTG CTAGGAGATT ACCAGGTGAA AATGTCAAAT CCAACAGAGC ATCACAGGAC
    TATGATCCGC TTCCTTCATC TTCAGATGGT TCACAAGCAC CAGCCAGACC TCCTAAACCA
    CGACCCCGCA GGACTGCACC AGAAATTCAC CACAGAAAAC CCCACGGGCC TGAGGCAGCA
    TTGGAAAATG TCGATGCAAA AATTGCAAAA CTCATGGGAG AGGGTTATGC CTTTGAAGAA
    GTGAAAAGAG CCTTAGAGAT AGCCCAGAAT AACGTGGAAG TGGCCCGTAG CATCCTTCGG
    GAATTTGCCT TCCCCCCTCC AGTCTCCCCA CGTCTAAATC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845508.1
    CDS237..3041
    Translation

    Target ORF information:

    RefSeq Version XM_024989740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGAGATGTT
    TTTGATTCAG CCTCTGATCC AGTGCCATTG CCACCTGCCA GGCCTCCAAC TCGGGACAAT
    CCAAAGCATG GTTCTTCACT CAACAGGACG CCCTCTGATT ATGATCTTCT CATCCCTCCA
    TTAGGCGAAG ATGCTTTTGA CGCTCTGCCT CCGTCCCTCC CACCTCCCCC ACCTCCCGCA
    AGGCACAGTC TCATCGAACA TTCGAAACCT CCTGGCTCTG GCAGCCGACC ATCCTCAGGA
    CAGGACCTTT TCCTCCTTCC TTCAGATCCC TTTTTTGATC CGGCAAGTGG CCAAGTTCCT
    TTGCCTCCTG CTAGGAGATT ACCAGGTGAA AATGTCAAAT CCAACAGAGC ATCACAGGAC
    TATGATCCGC TTCCTTCATC TTCAGATGGT TCACAAGCAC CAGCCAGACC TCCTAAACCA
    CGACCCCGCA GGACTGCACC AGAAATTCAC CACAGAAAAC CCCACGGGCC TGAGGCAGCA
    TTGGAAAATG TCGATGCAAA AATTGCAAAA CTCATGGGAG AGGGTTATGC CTTTGAAGAA
    GTGAAAAGAG CCTTAGAGAT AGCCCAGAAT AACGTGGAAG TGGCCCGTAG CATCCTTCGG
    GAATTTGCCT TCCCCCCTCC AGTCTCCCCA CGTCTAAATC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218775
    Clone ID Related Accession (Same CDS sequence) XM_024989740.1 , XM_024989731.1
    Accession Version XM_024989731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGAGATGTT
    TTTGATTCAG CCTCTGATCC AGTGCCATTG CCACCTGCCA GGCCTCCAAC TCGGGACAAT
    CCAAAGCATG GTTCTTCACT CAACAGGACG CCCTCTGATT ATGATCTTCT CATCCCTCCA
    TTAGGCGAAG ATGCTTTTGA CGCTCTGCCT CCGTCCCTCC CACCTCCCCC ACCTCCCGCA
    AGGCACAGTC TCATCGAACA TTCGAAACCT CCTGGCTCTG GCAGCCGACC ATCCTCAGGA
    CAGGACCTTT TCCTCCTTCC TTCAGATCCC TTTTTTGATC CGGCAAGTGG CCAAGTTCCT
    TTGCCTCCTG CTAGGAGATT ACCAGGTGAA AATGTCAAAT CCAACAGAGC ATCACAGGAC
    TATGATCCGC TTCCTTCATC TTCAGATGGT TCACAAGCAC CAGCCAGACC TCCTAAACCA
    CGACCCCGCA GGACTGCACC AGAAATTCAC CACAGAAAAC CCCACGGGCC TGAGGCAGCA
    TTGGAAAATG TCGATGCAAA AATTGCAAAA CTCATGGGAG AGGGTTATGC CTTTGAAGAA
    GTGAAAAGAG CCTTAGAGAT AGCCCAGAAT AACGTGGAAG TGGCCCGTAG CATCCTTCGG
    GAATTTGCCT TCCCCCCTCC AGTCTCCCCA CGTCTAAATC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845499.1
    CDS174..2978
    Translation

    Target ORF information:

    RefSeq Version XM_024989731.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGAGATGTT
    TTTGATTCAG CCTCTGATCC AGTGCCATTG CCACCTGCCA GGCCTCCAAC TCGGGACAAT
    CCAAAGCATG GTTCTTCACT CAACAGGACG CCCTCTGATT ATGATCTTCT CATCCCTCCA
    TTAGGCGAAG ATGCTTTTGA CGCTCTGCCT CCGTCCCTCC CACCTCCCCC ACCTCCCGCA
    AGGCACAGTC TCATCGAACA TTCGAAACCT CCTGGCTCTG GCAGCCGACC ATCCTCAGGA
    CAGGACCTTT TCCTCCTTCC TTCAGATCCC TTTTTTGATC CGGCAAGTGG CCAAGTTCCT
    TTGCCTCCTG CTAGGAGATT ACCAGGTGAA AATGTCAAAT CCAACAGAGC ATCACAGGAC
    TATGATCCGC TTCCTTCATC TTCAGATGGT TCACAAGCAC CAGCCAGACC TCCTAAACCA
    CGACCCCGCA GGACTGCACC AGAAATTCAC CACAGAAAAC CCCACGGGCC TGAGGCAGCA
    TTGGAAAATG TCGATGCAAA AATTGCAAAA CTCATGGGAG AGGGTTATGC CTTTGAAGAA
    GTGAAAAGAG CCTTAGAGAT AGCCCAGAAT AACGTGGAAG TGGCCCGTAG CATCCTTCGG
    GAATTTGCCT TCCCCCCTCC AGTCTCCCCA CGTCTAAATC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218776
    Clone ID Related Accession (Same CDS sequence) XM_024989734.1
    Accession Version XM_024989734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGCGAAGAT
    GCTTTTGACG CTCTGCCTCC GTCCCTCCCA CCTCCCCCAC CTCCCGCAAG GCACAGTCTC
    ATCGAACATT CGAAACCTCC TGGCTCTGGC AGCCGACCAT CCTCAGGACA GGACCTTTTC
    CTCCTTCCTT CAGATCCCTT TTTTGATCCG GCAAGTGGCC AAGTTCCTTT GCCTCCTGCT
    AGGAGATTAC CAGGTGAAAA TGTCAAATCC AACAGAGCAT CACAGGACTA TGATCCGCTT
    CCTTCATCTT CAGATGGTTC ACAAGCACCA GCCAGACCTC CTAAACCACG ACCCCGCAGG
    ACTGCACCAG AAATTCACCA CAGAAAACCC CACGGGCCTG AGGCAGCATT GGAAAATGTC
    GATGCAAAAA TTGCAAAACT CATGGGAGAG GGTTATGCCT TTGAAGAAGT GAAAAGAGCC
    TTAGAGATAG CCCAGAATAA CGTGGAAGTG GCCCGTAGCA TCCTTCGGGA ATTTGCCTTC
    CCCCCTCCAG TCTCCCCACG TCTAAATCTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845502.1
    CDS174..2846
    Translation

    Target ORF information:

    RefSeq Version XM_024989734.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGTCTTCTC CTTGCATGGT GAGAAAACAA
    GATAAACCAC TCCCAGCCCC ACCACCGCCC TTAAGAGACC CTCCTCCCCC TCCACCCGAG
    AGACCTCCCC CAGTCCCCCC TGACAACAGA CTGAGTCGTC ACTTCCATCA CGTGGAAAGT
    GTGCCTTCCA GAGATCAGCC CATGCCTCTT GAAGCCTGGT GCCCGCGGGA TGTGCTGGGG
    ACTAATCAGT CAGTGGGATG TCGTCTCCTT GGGGATGGCT CTCCGAAGCC TGGAATCACG
    GCAAGTTCCA ATGCAAATGG AAGGCATAGT CGGATGGGCT CTGACCCAGT GCTTCTCCGG
    AAACACAGAC GCAATGACTT GCCTTTAGAA GGAGCCAAGG TCTTTTCCAA CGGTCACCTG
    GGAAGTGAAG AATATGATGT TCCTCCCCGG CTTTCTCCTC CCCCTCCAGC TGCCACCCTT
    CTCCCCAGCA TCAAATCTTG TGATAACGGT CACTGTATAT TGAATGGAAC ACATGGCACA
    TCCTCAGAGA TGAAGAAATC AAACATCCCT GAGTTAGGCA TATATTTAAA GGGCGAAGAT
    GCTTTTGACG CTCTGCCTCC GTCCCTCCCA CCTCCCCCAC CTCCCGCAAG GCACAGTCTC
    ATCGAACATT CGAAACCTCC TGGCTCTGGC AGCCGACCAT CCTCAGGACA GGACCTTTTC
    CTCCTTCCTT CAGATCCCTT TTTTGATCCG GCAAGTGGCC AAGTTCCTTT GCCTCCTGCT
    AGGAGATTAC CAGGTGAAAA TGTCAAATCC AACAGAGCAT CACAGGACTA TGATCCGCTT
    CCTTCATCTT CAGATGGTTC ACAAGCACCA GCCAGACCTC CTAAACCACG ACCCCGCAGG
    ACTGCACCAG AAATTCACCA CAGAAAACCC CACGGGCCTG AGGCAGCATT GGAAAATGTC
    GATGCAAAAA TTGCAAAACT CATGGGAGAG GGTTATGCCT TTGAAGAAGT GAAAAGAGCC
    TTAGAGATAG CCCAGAATAA CGTGGAAGTG GCCCGTAGCA TCCTTCGGGA ATTTGCCTTC
    CCCCCTCCAG TCTCCCCACG TCTAAATCTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218777
    Clone ID Related Accession (Same CDS sequence) XM_024989738.1
    Accession Version XM_024989738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGGTCTTTT CCAACGGTCA CCTGGGAAGT
    GAAGAATATG ATGTTCCTCC CCGGCTTTCT CCTCCCCCTC CAGCTGCCAC CCTTCTCCCC
    AGCATCAAGT GTACTGGTCC ACTAGCGAAT TCCCTTTCAG AGAAAACAAG AGACCCGGTA
    GAGGAAGATG ACGATGAATA CAAGGTTCCT TCATCCCATC CTGTTTCCTT GAGTTCACAA
    CCATCTCATT GCCATAATGT AAAACCTCCT CCTAGATCTT GTGATAACGG TCACTGTATA
    TTGAATGGAA CACATGGCAC ATCCTCAGAG ATGAAGAAAT CAAACATCCC TGAGTTAGGC
    ATATATTTAA AGGGAGATGT TTTTGATTCA GCCTCTGATC CAGTGCCATT GCCACCTGCC
    AGGCCTCCAA CTCGGGACAA TCCAAAGCAT GGTTCTTCAC TCAACAGGAC GCCCTCTGAT
    TATGATCTTC TCATCCCTCC ATTAGGCGAA GATGCTTTTG ACGCTCTGCC TCCGTCCCTC
    CCACCTCCCC CACCTCCCGC AAGGCACAGT CTCATCGAAC ATTCGAAACC TCCTGGCTCT
    GGCAGCCGAC CATCCTCAGG ACAGGACCTT TTCCTCCTTC CTTCAGATCC CTTTTTTGAT
    CCGGCAAGTG GCCAAGTTCC TTTGCCTCCT GCTAGGAGAT TACCAGGTGA AAATGTCAAA
    TCCAACAGAG CATCACAGGA CTATGATCCG CTTCCTTCAT CTTCAGATGG TTCACAAGCA
    CCAGCCAGAC CTCCTAAACC ACGACCCCGC AGGACTGCAC CAGAAATTCA CCACAGAAAA
    CCCCACGGGC CTGAGGCAGC ATTGGAAAAT GTCGATGCAA AAATTGCAAA ACTCATGGGA
    GAGGGTTATG CCTTTGAAGA AGTGAAAAGA GCCTTAGAGA TAGCCCAGAA TAACGTGGAA
    GTGGCCCGTA GCATCCTTCG GGAATTTGCC TTCCCCCCTC CAGTCTCCCC ACGTCTAAAT
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845506.1
    CDS174..2759
    Translation

    Target ORF information:

    RefSeq Version XM_024989738.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGGTCTTTT CCAACGGTCA CCTGGGAAGT
    GAAGAATATG ATGTTCCTCC CCGGCTTTCT CCTCCCCCTC CAGCTGCCAC CCTTCTCCCC
    AGCATCAAGT GTACTGGTCC ACTAGCGAAT TCCCTTTCAG AGAAAACAAG AGACCCGGTA
    GAGGAAGATG ACGATGAATA CAAGGTTCCT TCATCCCATC CTGTTTCCTT GAGTTCACAA
    CCATCTCATT GCCATAATGT AAAACCTCCT CCTAGATCTT GTGATAACGG TCACTGTATA
    TTGAATGGAA CACATGGCAC ATCCTCAGAG ATGAAGAAAT CAAACATCCC TGAGTTAGGC
    ATATATTTAA AGGGAGATGT TTTTGATTCA GCCTCTGATC CAGTGCCATT GCCACCTGCC
    AGGCCTCCAA CTCGGGACAA TCCAAAGCAT GGTTCTTCAC TCAACAGGAC GCCCTCTGAT
    TATGATCTTC TCATCCCTCC ATTAGGCGAA GATGCTTTTG ACGCTCTGCC TCCGTCCCTC
    CCACCTCCCC CACCTCCCGC AAGGCACAGT CTCATCGAAC ATTCGAAACC TCCTGGCTCT
    GGCAGCCGAC CATCCTCAGG ACAGGACCTT TTCCTCCTTC CTTCAGATCC CTTTTTTGAT
    CCGGCAAGTG GCCAAGTTCC TTTGCCTCCT GCTAGGAGAT TACCAGGTGA AAATGTCAAA
    TCCAACAGAG CATCACAGGA CTATGATCCG CTTCCTTCAT CTTCAGATGG TTCACAAGCA
    CCAGCCAGAC CTCCTAAACC ACGACCCCGC AGGACTGCAC CAGAAATTCA CCACAGAAAA
    CCCCACGGGC CTGAGGCAGC ATTGGAAAAT GTCGATGCAA AAATTGCAAA ACTCATGGGA
    GAGGGTTATG CCTTTGAAGA AGTGAAAAGA GCCTTAGAGA TAGCCCAGAA TAACGTGGAA
    GTGGCCCGTA GCATCCTTCG GGAATTTGCC TTCCCCCCTC CAGTCTCCCC ACGTCTAAAT
    CTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218778
    Clone ID Related Accession (Same CDS sequence) XM_024989742.1
    Accession Version XM_024989742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase CBL-B isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGGTCTTTT CCAACGGTCA CCTGGGAAGT
    GAAGAATATG ATGTTCCTCC CCGGCTTTCT CCTCCCCCTC CAGCTGCCAC CCTTCTCCCC
    AGCATCAAAT CTTGTGATAA CGGTCACTGT ATATTGAATG GAACACATGG CACATCCTCA
    GAGATGAAGA AATCAAACAT CCCTGAGTTA GGCATATATT TAAAGGGAGA TGTTTTTGAT
    TCAGCCTCTG ATCCAGTGCC ATTGCCACCT GCCAGGCCTC CAACTCGGGA CAATCCAAAG
    CATGGTTCTT CACTCAACAG GACGCCCTCT GATTATGATC TTCTCATCCC TCCATTAGGC
    GAAGATGCTT TTGACGCTCT GCCTCCGTCC CTCCCACCTC CCCCACCTCC CGCAAGGCAC
    AGTCTCATCG AACATTCGAA ACCTCCTGGC TCTGGCAGCC GACCATCCTC AGGACAGGAC
    CTTTTCCTCC TTCCTTCAGA TCCCTTTTTT GATCCGGCAA GTGGCCAAGT TCCTTTGCCT
    CCTGCTAGGA GATTACCAGG TGAAAATGTC AAATCCAACA GAGCATCACA GGACTATGAT
    CCGCTTCCTT CATCTTCAGA TGGTTCACAA GCACCAGCCA GACCTCCTAA ACCACGACCC
    CGCAGGACTG CACCAGAAAT TCACCACAGA AAACCCCACG GGCCTGAGGC AGCATTGGAA
    AATGTCGATG CAAAAATTGC AAAACTCATG GGAGAGGGTT ATGCCTTTGA AGAAGTGAAA
    AGAGCCTTAG AGATAGCCCA GAATAACGTG GAAGTGGCCC GTAGCATCCT TCGGGAATTT
    GCCTTCCCCC CTCCAGTCTC CCCACGTCTA AATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845510.1
    CDS174..2612
    Translation

    Target ORF information:

    RefSeq Version XM_024989742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Cbl proto-oncogene B (CBLB), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAAATT CTATGAATGG CAGAAACCCT GGTGGTCGAG GAGGAAACCC CCGAAAAGGA 
    CGGATTTTGG GTATTATTGA TGCTATTCAA GATGCAGTTG GACCCCCTAA GCAAGCCGCA
    GCGGATCGCA GGACAGTAGA GAAAACTTGG AAACTCATGG ACAAAGTGGT CAGACTGTGC
    CAAAACCCCA AACTTCAGTT GAAAAATAGC CCACCATATA TACTTGATAT TTTACCTGAT
    ACATATCAGC ATTTGAGACT TATATTGAGT AAATATGATG ACAACCAGAA ACTTGCCCAA
    CTCAGTGAGA ATGAATATTT TAAAATCTAC ATTGATAGTC TAATGAAAAA GTCAAAAAGG
    GCAATAAGGC TCTTTAAAGA AGGCAAGGAG AGGATGTATG AAGAGCAGTC GCAGGACAGA
    CGAAATCTCA CAAAACTATC CCTTATCTTC AGTCACATGT TGGCAGAAAT CAAAGCAATC
    TTTCCCAATG GACAGTTCCA GGGAGATAAC TTTCGTATCA CGAAAGCAGA TGCTGCTGAA
    TTCTGGAGAA AGTTTTTTGG AGACAAAACT ATTGTACCAT GGAAAGTATT CAGACAATGC
    CTTCATGAGG TGCATCAGAT TAGCTCTGGC CTGGAAGCAA TGGCTCTAAA ATCAACCATT
    GACTTGACTT GTAATGATTA CATTTCAGTT TTTGAATTTG ATATTTTTAC CAGGCTATTT
    CAGCCTTGGG GTTCTATTTT ACGGAATTGG AATTTCTTAG CTGTAACACA TCCGGGTTAC
    ATGGCATTTC TCACATATGA TGAAGTAAAA GCACGGCTAC AGAAATACAG CACCAAACCC
    GGAAGCTACA TTTTCCGGTT AAGCTGCACC AGGCTGGGGC AGTGGGCCAT TGGCTATGTG
    ACAGGGGATG GCAATATCTT ACAGACCATA CCGCACAACA AGCCCTTATT TCAAGCCTTG
    ATTGATGGCA GCAGGGAAGG ATTTTATCTT TATCCTGATG GGAGGAGTTA TAATCCTGAT
    TTAACTGGAT TATGTGAACC TACACCCCAT GATCATATAA AAGTTACACA GGAACAATAT
    GAATTATATT GTGAGATGGG TTCCACTTTC CAGCTCTGTA AGATTTGTGC TGAGAACGAC
    AAGGATGTCA AGATCGAGCC TTGTGGGCAC CTGATGTGCA CCTCTTGTCT TACAGCCTGG
    CAGGAGTCAG ACGGCCAGGG CTGCCCATTC TGTCGCTGCG AAATAAAAGG AACGGAGCCC
    ATCATCGTGG ACCCCTTTGA TCCGAGGGAT GAAGGATCAA GGTGCTGTAG CATCATCGAC
    CCTTTTGGAA TGCCGATGCT GGACTTGGAC GATGATGATG ACCGGGAAGA GTCCTTGATG
    ATGAATCGGT TGGCAAATGT TCGAAAGTGC ACTGACAGGC AGAATTCCCC AGTCACGTCA
    CCTGGATCCT CTCCCCTGGC ACAGAGAAGA AAGCCACACC CAGACCCGCT CCAGATCCCA
    CATCTAAGCC TCCCACCGGT ACCTCCCCGC CTGGACCTAA TTCAGAAAGG CATAGTTCGG
    TCTCCCTGTG GCAGCCCAAC TGGTTCACCA AAGGTCTTTT CCAACGGTCA CCTGGGAAGT
    GAAGAATATG ATGTTCCTCC CCGGCTTTCT CCTCCCCCTC CAGCTGCCAC CCTTCTCCCC
    AGCATCAAAT CTTGTGATAA CGGTCACTGT ATATTGAATG GAACACATGG CACATCCTCA
    GAGATGAAGA AATCAAACAT CCCTGAGTTA GGCATATATT TAAAGGGAGA TGTTTTTGAT
    TCAGCCTCTG ATCCAGTGCC ATTGCCACCT GCCAGGCCTC CAACTCGGGA CAATCCAAAG
    CATGGTTCTT CACTCAACAG GACGCCCTCT GATTATGATC TTCTCATCCC TCCATTAGGC
    GAAGATGCTT TTGACGCTCT GCCTCCGTCC CTCCCACCTC CCCCACCTCC CGCAAGGCAC
    AGTCTCATCG AACATTCGAA ACCTCCTGGC TCTGGCAGCC GACCATCCTC AGGACAGGAC
    CTTTTCCTCC TTCCTTCAGA TCCCTTTTTT GATCCGGCAA GTGGCCAAGT TCCTTTGCCT
    CCTGCTAGGA GATTACCAGG TGAAAATGTC AAATCCAACA GAGCATCACA GGACTATGAT
    CCGCTTCCTT CATCTTCAGA TGGTTCACAA GCACCAGCCA GACCTCCTAA ACCACGACCC
    CGCAGGACTG CACCAGAAAT TCACCACAGA AAACCCCACG GGCCTGAGGC AGCATTGGAA
    AATGTCGATG CAAAAATTGC AAAACTCATG GGAGAGGGTT ATGCCTTTGA AGAAGTGAAA
    AGAGCCTTAG AGATAGCCCA GAATAACGTG GAAGTGGCCC GTAGCATCCT TCGGGAATTT
    GCCTTCCCCC CTCCAGTCTC CCCACGTCTA AATCTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.