ZNF227 cDNA ORF clone, Bos taurus(cattle)

The following ZNF227 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF227 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225049 XM_024978300.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978299.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978295.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978293.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978297.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978292.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978298.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978296.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225049 XM_024978294.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225050 XM_024978301.1
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa42450 NM_001077960.2
Latest version!
Bos taurus zinc finger protein 227 (ZNF227), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OBa42450 NM_001077960.1 Bos taurus zinc finger protein 227 (ZNF227), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834062.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978294.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978299.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834067.1
    CDS197..2707
    Translation

    Target ORF information:

    RefSeq Version XM_024978299.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978300.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834068.1
    CDS167..2677
    Translation

    Target ORF information:

    RefSeq Version XM_024978300.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834063.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978295.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834061.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978293.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834064.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978296.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834066.1
    CDS212..2722
    Translation

    Target ORF information:

    RefSeq Version XM_024978298.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834060.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978292.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225049
    Clone ID Related Accession (Same CDS sequence) XM_024978294.1 , XM_024978299.1 , XM_024978300.1 , XM_024978295.1 , XM_024978293.1 , XM_024978296.1 , XM_024978298.1 , XM_024978292.1 , XM_024978297.1
    Accession Version XM_024978297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834065.1
    CDS157..2667
    Translation

    Target ORF information:

    RefSeq Version XM_024978297.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTCCGCTG CAGTTTATAC AGGAAACGTG AGACGAACAC AGTCATTCTG TCCCACCGCA 
    TCGTTTTCAT CGCCCGTCTT GAATCCGGAA GGGAGGGAAA GGAAGGACTT CCTGCCTCCT
    CAGAGCATAG CCGCAGTTAT GCCTTCTCAG GACTCTGACT TTCCCCAGAA GGAACATGAG
    AAAATGACCG AGTTTCAGGA GGCCGTGACC TTCAAGGATG TGGCTGTGGT CTTCACTGAG
    GAGGAGCTGG GGTTACTGGA CTCGGCCCAG AGGAAGCTGT ACCAGGATGT GATGGTGGAG
    AACTTCCGGA ACCTGGTCTC AGTGGGACAT GTTCCATTCA AAGCAGATAT GGTATCCCAG
    TTGGAGACAG AAGAAAAACT GTGGACAATG GAGAGGGAAA CCCAAAGAAA TGGGCATTCC
    AGTGAGATCC ACTCAGCTGT TACTTCAGGC AGCAAGATCC CAAATGAGAT GGAGACTCTT
    GGGAAAGTCA TGTTCAAATA CCTCTCATGT GAAGAACTGT CCTGCTGGCA AATCTGGAAA
    CACACCACAA ATGATTTAAC TCTGCAAAGG AAGAGTTCCT GGTTCCTGCA TGGTGATTCT
    CTTCAAGTTT CTGAAGATGA GAACCATATA ATGAATCATA AAGGAGATCA CTTCGGTTGC
    CTTGAGAATC AGGAGTTTTT GATTCCGACA GCCCAGGCTT CCTGTGGGAG TAGGTGTCTG
    AGCGAGTCAG AGAATCCAAG CAGAGGTAAA CAGATGAGTG TGAAAAATCA TCTGCATGTA
    TGTGAAGGCT TCACAAAGAA TTCACCGCTG AGCGATCCTG GTAAAACTGA CGCAAAACAG
    ACACCCTGCA AAGGAGAGAG ACCGCATCCC TGTAGAGTGT GTGGGGAGGG CTTCAGTCAT
    GGTGCAGTGC TCCCAGTTCA TCAGGTTGAC CCTGGAGAAA AATGCTCCCA TCTGCAGACT
    CATCAGAGAA TTCACCCAGG AGGGACTGTC AATAAATGTC CTAAATCTGG CGATGGTTTC
    CATCAGAACT CCTTTCACCC CCATCACTCC AACCCCACAG GAGAAAAGTC CTACAGGTGT
    GACAGTTGTG GCAAGGCCTT TGGTAGCAGC ACAGGCCTCA TCATCCATTA CCGGACGCAC
    ACAGGGGAGA AACCTTACAG GTGCGAGGCG TGCGGCAAAT GCTTCAGCCA GAGTTCCAAT
    TTTCAGTGCC ATCAGAGAGT CCACACGGAA GAGAAGCCCT ACAAGTGTGA AGAGTGTGGC
    AAGGGCTTCG GCTGGAGCGT CAACCTCCGT GTTCACCAGC GGGTCCACAG GGGCGAGAAA
    CCCTACAAGT GTGAGGAGTG CGGGAAGGGC TTCACGCAGG CCGCCCATTA CCACATACAC
    CAGAGGGTCC ACACCGGGGA GAAGCCCTAT AAGTGCGACG TCTGCGGCAA GGGGTTCAGT
    CACAACTCGC CTCTGATTTG CCACCGGCGC GTCCACACTG GTGAGAAGCC CTACCGGTGC
    GAGGCCTGCG GCAAAGGCTT CACCCGGAAC ACGGACCTCC ACATCCACTT CCGCGTCCAT
    ACAGGGGAGA AGCCCTACAC CTGCAAGGAG TGTGGGAAGG GCTTCAGTCA GGCTTCAAAT
    CTTCAAGTCC ACCAGAACGT CCACACTGGG GAGAAGCGGT TCAAGTGCGA GACATGCGGG
    AAGGGCTTCA GCCAGTCGTC CAAGCTCCAG ACCCACCAGC GAGTCCACAC CGGGGAGAAG
    CCCTACAGGT GCGACGTGTG TGGCAAGGAC TTCAGTTACA GTTCCAACCT GAAGCTGCAC
    CAGGTCATTC ACACCGGAGA GAAACCATAC ACCTGCGAGG CATGCGGGAA GGGCTTCAGC
    TGGAGGTCCA ACCTTCATGC TCATCAGCGA GTCCACTCGG GAGAGAAGCC CTACAAATGT
    GAGGCGTGTG ACAAGAGCTT CAGTCAGGCC ATAGACTTCC GGGTCCATCA GCGGGTCCAC
    ACAGGCGAGA AACCCTACAA GTGTGGTGTC TGCGGGAAGG GCTTCAGCCA GTCCTCGGGT
    CTTCAGTCCC ATCAGAGGGT CCACACCGGG GAGAAGCCCT ACAAATGCGA TGTGTGTGGG
    AAGGGCTTCC GATACAGTTC CCAGTTTATT TACCACCAGA GGGGCCACAC TGGCGAAAAG
    CCTTACAAGT GTGAGGAGTG CGGGAAAGGC TTTGGGCGGA GCCTGAACCT TCGCCACCAC
    CAGAGGGTCC ACACAGGAGA GAAACCCCAC AAGTGTGAGG AGTGCGGGAA GGCCTTCAGT
    CTCCCCTCCA ATCTTCGAGT CCACCTGAGC GTCCACACCC GGGAGAAACT GTTTAAATGT
    GAGGATTGTG GGAAGGGCTT CAGTCAGAGT TCTCGGCTTC AAGCCCACCA GAGGGTCCAC
    ACAGGAGAAA AACCGTACAA GTGCAACATA TGCGTGCTAA TTGTTAATGG ATGTTGGAGG
    ATATCCAGAA AACATTCACC TGGAGAAACA AAGCATGGGA TTTTTGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225050
    Clone ID Related Accession (Same CDS sequence) XM_024978301.1
    Accession Version XM_024978301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product zinc finger protein 227 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGTGGAGA ACTTCCGGAA CCTGGTCTCA GTGGGACATG TTCCATTCAA AGCAGATATG 
    GTATCCCAGT TGGAGACAGA AGAAAAACTG TGGACAATGG AGAGGGAAAC CCAAAGAAAT
    GGGCATTCCA GTGAGATCCA CTCAGCTGTT ACTTCAGGCA GCAAGATCCC AAATGAGATG
    GAGACTCTTG GGAAAGTCAT GTTCAAATAC CTCTCATGTG AAGAACTGTC CTGCTGGCAA
    ATCTGGAAAC ACACCACAAA TGATTTAACT CTGCAAAGGA AGAGTTCCTG GTTCCTGCAT
    GGTGATTCTC TTCAAGTTTC TGAAGATGAG AACCATATAA TGAATCATAA AGGAGATCAC
    TTCGGTTGCC TTGAGAATCA GGAGTTTTTG ATTCCGACAG CCCAGGCTTC CTGTGGGAGT
    AGGTGTCTGA GCGAGTCAGA GAATCCAAGC AGAGGTAAAC AGATGAGTGT GAAAAATCAT
    CTGCATGTAT GTGAAGGCTT CACAAAGAAT TCACCGCTGA GCGATCCTGG TAAAACTGAC
    GCAAAACAGA CACCCTGCAA AGGAGAGAGA CCGCATCCCT GTAGAGTGTG TGGGGAGGGC
    TTCAGTCATG GTGCAGTGCT CCCAGTTCAT CAGGTTGACC CTGGAGAAAA ATGCTCCCAT
    CTGCAGACTC ATCAGAGAAT TCACCCAGGA GGGACTGTCA ATAAATGTCC TAAATCTGGC
    GATGGTTTCC ATCAGAACTC CTTTCACCCC CATCACTCCA ACCCCACAGG AGAAAAGTCC
    TACAGGTGTG ACAGTTGTGG CAAGGCCTTT GGTAGCAGCA CAGGCCTCAT CATCCATTAC
    CGGACGCACA CAGGGGAGAA ACCTTACAGG TGCGAGGCGT GCGGCAAATG CTTCAGCCAG
    AGTTCCAATT TTCAGTGCCA TCAGAGAGTC CACACGGAAG AGAAGCCCTA CAAGTGTGAA
    GAGTGTGGCA AGGGCTTCGG CTGGAGCGTC AACCTCCGTG TTCACCAGCG GGTCCACAGG
    GGCGAGAAAC CCTACAAGTG TGAGGAGTGC GGGAAGGGCT TCACGCAGGC CGCCCATTAC
    CACATACACC AGAGGGTCCA CACCGGGGAG AAGCCCTATA AGTGCGACGT CTGCGGCAAG
    GGGTTCAGTC ACAACTCGCC TCTGATTTGC CACCGGCGCG TCCACACTGG TGAGAAGCCC
    TACCGGTGCG AGGCCTGCGG CAAAGGCTTC ACCCGGAACA CGGACCTCCA CATCCACTTC
    CGCGTCCATA CAGGGGAGAA GCCCTACACC TGCAAGGAGT GTGGGAAGGG CTTCAGTCAG
    GCTTCAAATC TTCAAGTCCA CCAGAACGTC CACACTGGGG AGAAGCGGTT CAAGTGCGAG
    ACATGCGGGA AGGGCTTCAG CCAGTCGTCC AAGCTCCAGA CCCACCAGCG AGTCCACACC
    GGGGAGAAGC CCTACAGGTG CGACGTGTGT GGCAAGGACT TCAGTTACAG TTCCAACCTG
    AAGCTGCACC AGGTCATTCA CACCGGAGAG AAACCATACA CCTGCGAGGC ATGCGGGAAG
    GGCTTCAGCT GGAGGTCCAA CCTTCATGCT CATCAGCGAG TCCACTCGGG AGAGAAGCCC
    TACAAATGTG AGGCGTGTGA CAAGAGCTTC AGTCAGGCCA TAGACTTCCG GGTCCATCAG
    CGGGTCCACA CAGGCGAGAA ACCCTACAAG TGTGGTGTCT GCGGGAAGGG CTTCAGCCAG
    TCCTCGGGTC TTCAGTCCCA TCAGAGGGTC CACACCGGGG AGAAGCCCTA CAAATGCGAT
    GTGTGTGGGA AGGGCTTCCG ATACAGTTCC CAGTTTATTT ACCACCAGAG GGGCCACACT
    GGCGAAAAGC CTTACAAGTG TGAGGAGTGC GGGAAAGGCT TTGGGCGGAG CCTGAACCTT
    CGCCACCACC AGAGGGTCCA CACAGGAGAG AAACCCCACA AGTGTGAGGA GTGCGGGAAG
    GCCTTCAGTC TCCCCTCCAA TCTTCGAGTC CACCTGAGCG TCCACACCCG GGAGAAACTG
    TTTAAATGTG AGGATTGTGG GAAGGGCTTC AGTCAGAGTT CTCGGCTTCA AGCCCACCAG
    AGGGTCCACA CAGGAGAAAA ACCGTACAAG TGCAACATAT GCGTGCTAAT TGTTAATGGA
    TGTTGGAGGA TATCCAGAAA ACATTCACCT GGAGAAACAA AGCATGGGAT TTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834069.1
    CDS233..2452
    Translation

    Target ORF information:

    RefSeq Version XM_024978301.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGTGGAGA ACTTCCGGAA CCTGGTCTCA GTGGGACATG TTCCATTCAA AGCAGATATG 
    GTATCCCAGT TGGAGACAGA AGAAAAACTG TGGACAATGG AGAGGGAAAC CCAAAGAAAT
    GGGCATTCCA GTGAGATCCA CTCAGCTGTT ACTTCAGGCA GCAAGATCCC AAATGAGATG
    GAGACTCTTG GGAAAGTCAT GTTCAAATAC CTCTCATGTG AAGAACTGTC CTGCTGGCAA
    ATCTGGAAAC ACACCACAAA TGATTTAACT CTGCAAAGGA AGAGTTCCTG GTTCCTGCAT
    GGTGATTCTC TTCAAGTTTC TGAAGATGAG AACCATATAA TGAATCATAA AGGAGATCAC
    TTCGGTTGCC TTGAGAATCA GGAGTTTTTG ATTCCGACAG CCCAGGCTTC CTGTGGGAGT
    AGGTGTCTGA GCGAGTCAGA GAATCCAAGC AGAGGTAAAC AGATGAGTGT GAAAAATCAT
    CTGCATGTAT GTGAAGGCTT CACAAAGAAT TCACCGCTGA GCGATCCTGG TAAAACTGAC
    GCAAAACAGA CACCCTGCAA AGGAGAGAGA CCGCATCCCT GTAGAGTGTG TGGGGAGGGC
    TTCAGTCATG GTGCAGTGCT CCCAGTTCAT CAGGTTGACC CTGGAGAAAA ATGCTCCCAT
    CTGCAGACTC ATCAGAGAAT TCACCCAGGA GGGACTGTCA ATAAATGTCC TAAATCTGGC
    GATGGTTTCC ATCAGAACTC CTTTCACCCC CATCACTCCA ACCCCACAGG AGAAAAGTCC
    TACAGGTGTG ACAGTTGTGG CAAGGCCTTT GGTAGCAGCA CAGGCCTCAT CATCCATTAC
    CGGACGCACA CAGGGGAGAA ACCTTACAGG TGCGAGGCGT GCGGCAAATG CTTCAGCCAG
    AGTTCCAATT TTCAGTGCCA TCAGAGAGTC CACACGGAAG AGAAGCCCTA CAAGTGTGAA
    GAGTGTGGCA AGGGCTTCGG CTGGAGCGTC AACCTCCGTG TTCACCAGCG GGTCCACAGG
    GGCGAGAAAC CCTACAAGTG TGAGGAGTGC GGGAAGGGCT TCACGCAGGC CGCCCATTAC
    CACATACACC AGAGGGTCCA CACCGGGGAG AAGCCCTATA AGTGCGACGT CTGCGGCAAG
    GGGTTCAGTC ACAACTCGCC TCTGATTTGC CACCGGCGCG TCCACACTGG TGAGAAGCCC
    TACCGGTGCG AGGCCTGCGG CAAAGGCTTC ACCCGGAACA CGGACCTCCA CATCCACTTC
    CGCGTCCATA CAGGGGAGAA GCCCTACACC TGCAAGGAGT GTGGGAAGGG CTTCAGTCAG
    GCTTCAAATC TTCAAGTCCA CCAGAACGTC CACACTGGGG AGAAGCGGTT CAAGTGCGAG
    ACATGCGGGA AGGGCTTCAG CCAGTCGTCC AAGCTCCAGA CCCACCAGCG AGTCCACACC
    GGGGAGAAGC CCTACAGGTG CGACGTGTGT GGCAAGGACT TCAGTTACAG TTCCAACCTG
    AAGCTGCACC AGGTCATTCA CACCGGAGAG AAACCATACA CCTGCGAGGC ATGCGGGAAG
    GGCTTCAGCT GGAGGTCCAA CCTTCATGCT CATCAGCGAG TCCACTCGGG AGAGAAGCCC
    TACAAATGTG AGGCGTGTGA CAAGAGCTTC AGTCAGGCCA TAGACTTCCG GGTCCATCAG
    CGGGTCCACA CAGGCGAGAA ACCCTACAAG TGTGGTGTCT GCGGGAAGGG CTTCAGCCAG
    TCCTCGGGTC TTCAGTCCCA TCAGAGGGTC CACACCGGGG AGAAGCCCTA CAAATGCGAT
    GTGTGTGGGA AGGGCTTCCG ATACAGTTCC CAGTTTATTT ACCACCAGAG GGGCCACACT
    GGCGAAAAGC CTTACAAGTG TGAGGAGTGC GGGAAAGGCT TTGGGCGGAG CCTGAACCTT
    CGCCACCACC AGAGGGTCCA CACAGGAGAG AAACCCCACA AGTGTGAGGA GTGCGGGAAG
    GCCTTCAGTC TCCCCTCCAA TCTTCGAGTC CACCTGAGCG TCCACACCCG GGAGAAACTG
    TTTAAATGTG AGGATTGTGG GAAGGGCTTC AGTCAGAGTT CTCGGCTTCA AGCCCACCAG
    AGGGTCCACA CAGGAGAAAA ACCGTACAAG TGCAACATAT GCGTGCTAAT TGTTAATGGA
    TGTTGGAGGA TATCCAGAAA ACATTCACCT GGAGAAACAA AGCATGGGAT TTTTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa42450
    Clone ID Related Accession (Same CDS sequence) NM_001077960.1 , NM_001077960.2
    Accession Version NM_001077960.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-19
    Organism Bos taurus(cattle)
    Product zinc finger protein 227
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126592.1. On Nov 21, 2006 this sequence version replaced XM_602431.2. ##Evidence-Data-START## Transcript exon combination :: BC126592.1, EE352214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTTCTC AGGACTCTGA CTTTCCCCAG AAGGAACATG AGAAAATGAC CGAGTTTCAG 
    GAGGCCGTGA CCTTCAAGGA TGTGGCTGTG GTCTTCACTG AGGAGGAGCT GGGGTTACTG
    GACTCGGCCC AGAGGAAGCT GTACCAGGAT GTGATGGTGG AGAACTTCCG GAACCTGGTC
    TCAGTGGGAC ATGTTCCATT CAAAGCAGAT ATGGTATCCC AGTTGGAGAC AGAAGAAAAA
    CTGTGGACAA TGGAGAGGGA AACCCAAAGA AATGGGCATT CCAGTGAGAT CCACTCAGCT
    GTTACTTCAG GCAGCAAGAT CCCAAATGAG ATGGAGACTC TTGGGAAAGT CATGTTCAAA
    TACCTCTCGT GTGAAGAACT GTCCTGCTGG CAAATCTGGA AACACACCAC AAATGATTTA
    ACTCTGCAAA GGAAGAGTTC CTGGTTCCTG CATGGTGATT CTCTTCAAGT TTCTGAAGAT
    GAGAACCATA TAATGAATCA TAAAGGAGAT CACTTCGGTT GCCTTGAGAA TCAGGAGTTT
    TTGATTCCGA CAGCCCAGGC TTCCTGTGGG AGTAGGTGTC TGAGCGAGTC AGAGAATCCA
    AGCAGAGGTA AACAGATGAG TGTGAAAAAT CATCTGCATG TATGTGAAGG CTTCACAAAG
    AATTCACCGC TGAGCGATCC TGGTAAAACT GACGCAAAAC AGACACCCTG CAAAGGAGAG
    AGACCGCATC CCTGTAGAGT GTGTGGGGAG GGCTTCAGTC ATGGTGCAGT GCTCCCAGTT
    CATCAGGTTG ACCCTGGAGA AAAATGCTCC CATCTGCAGA CTCATCAGAG AATTCACCCA
    GGAGGGACTG TCAATAAATG TCCTAAATCT GGCGATGGTT TCCATCAGAA CTCCTTTCAC
    CCCCATCACT CCAACCCCAC AGGAGAAAAG TCCTACAGGT GTGACAGTTG TGGCAAGGCC
    TTTGGTAGCA GCACAGGCCT CATCATCCAT TACCGGACGC ACACAGGGGA GAAACCTTAC
    AGGTGCGAGG CGTGCGGCAA ATGCTTCAGC CAGAGTTCCA ATTTTCAGTG CCATCAGAGA
    GTCCACACGG AAGAGAAGCC CTACAAGTGT GAAGAGTGTG GCAAGGGCTT CGGCTGGAGC
    GTCAACCTCC GTGTTCACCA GCGGGTCCAC AGGGGCGAGA AACCCTACAA GTGTGAGGAG
    TGCGGGAAGG GCTTCACGCA GGCCGCCCAT TACCACATAC ACCAGAGGGT CCACACCGGG
    GAGAAGCCCT ATAAGTGCGA CGTCTGCGGC AAGGGGTTCA GTCACAACTC GCCTCTGATT
    TGCCACCGGC GCGTCCACAC TGGTGAGAAG CCCTACCGGT GCGAGGCCTG CGGCAAAGGC
    TTCACCCGGA ACACGGACCT CCACATCCAC TTCCGCGTCC ATACAGGGGA GAAGCCCTAC
    ACCTGCAAGG AGTGTGGGAA GGGCTTCAGT CAGGCTTCAA ATCTTCAAGT CCACCAGAAC
    GTCCACACTG GGGAGAAGCG GTTCAAGTGC GAGACATGCG GGAAGGGCTT CAGCCAGTCG
    TCCAAGCTCC AGACCCACCA GCGAGTCCAC ACCGGGGAGA AGCCCTACAG GTGCGACGTG
    TGTGGCAAGG ACTTCAGTTA CAGTTCCAAC CTGAAGCTGC ACCAGGTCAT TCACACCGGA
    GAGAAACCAT ACACCTGCGA GGCATGCGGG AAGGGCTTCA GCTGGAGGTC CAACCTTCAT
    GCTCATCAGC GAGTCCACTC GGGAGAGAAG CCCTACAAAT GTGAGGCGTG TGACAAGAGC
    TTCAGTCAGG CCATAGACTT CCGGGTCCAT CAGCGGGTCC ACACAGGCGA GAAACCCTAC
    AAGTGTGGTG TCTGCGGGAA GGGCTTCAGC CAGTCCTCGG GTCTTCAGTC CCATCAGAGG
    GTCCACACCG GGGAGAAGCC CTACAAATGC GATGTGTGTG GGAAGGGCTT CCGATACAGT
    TCCCAGTTTA TTTACCACCA GAGGGGCCAC ACTGGCGAAA AGCCTTACAA GTGTGAGGAG
    TGCGGGAAAG GCTTTGGGCG GAGCCTGAAC CTTCGCCACC ACCAGAGGGT CCACACAGGA
    GAGAAACCCC ACAAGTGTGA GGAGTGCGGG AAGGCCTTCA GTCTCCCCTC CAATCTTCGA
    GTCCACCTGA GCGTCCACAC CCGGGAGAAA CTGTTTAAAT GTGAGGATTG TGGGAAGGGC
    TTCAGTCAGA GTTCTCGGCT TCAAGCCCAC CAGAGGGTCC ACACAGGAGA AAAACCGTAC
    AAGTGCAACA TATGCGGTAA GGACTTCAGT CACCGTTCAC GGCTGACATA CCATCAGAAA
    GTCCACACTG GCAAGAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071428.1
    CDS230..2593
    Translation

    Target ORF information:

    RefSeq Version NM_001077960.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTTCTC AGGACTCTGA CTTTCCCCAG AAGGAACATG AGAAAATGAC CGAGTTTCAG 
    GAGGCCGTGA CCTTCAAGGA TGTGGCTGTG GTCTTCACTG AGGAGGAGCT GGGGTTACTG
    GACTCGGCCC AGAGGAAGCT GTACCAGGAT GTGATGGTGG AGAACTTCCG GAACCTGGTC
    TCAGTGGGAC ATGTTCCATT CAAAGCAGAT ATGGTATCCC AGTTGGAGAC AGAAGAAAAA
    CTGTGGACAA TGGAGAGGGA AACCCAAAGA AATGGGCATT CCAGTGAGAT CCACTCAGCT
    GTTACTTCAG GCAGCAAGAT CCCAAATGAG ATGGAGACTC TTGGGAAAGT CATGTTCAAA
    TACCTCTCGT GTGAAGAACT GTCCTGCTGG CAAATCTGGA AACACACCAC AAATGATTTA
    ACTCTGCAAA GGAAGAGTTC CTGGTTCCTG CATGGTGATT CTCTTCAAGT TTCTGAAGAT
    GAGAACCATA TAATGAATCA TAAAGGAGAT CACTTCGGTT GCCTTGAGAA TCAGGAGTTT
    TTGATTCCGA CAGCCCAGGC TTCCTGTGGG AGTAGGTGTC TGAGCGAGTC AGAGAATCCA
    AGCAGAGGTA AACAGATGAG TGTGAAAAAT CATCTGCATG TATGTGAAGG CTTCACAAAG
    AATTCACCGC TGAGCGATCC TGGTAAAACT GACGCAAAAC AGACACCCTG CAAAGGAGAG
    AGACCGCATC CCTGTAGAGT GTGTGGGGAG GGCTTCAGTC ATGGTGCAGT GCTCCCAGTT
    CATCAGGTTG ACCCTGGAGA AAAATGCTCC CATCTGCAGA CTCATCAGAG AATTCACCCA
    GGAGGGACTG TCAATAAATG TCCTAAATCT GGCGATGGTT TCCATCAGAA CTCCTTTCAC
    CCCCATCACT CCAACCCCAC AGGAGAAAAG TCCTACAGGT GTGACAGTTG TGGCAAGGCC
    TTTGGTAGCA GCACAGGCCT CATCATCCAT TACCGGACGC ACACAGGGGA GAAACCTTAC
    AGGTGCGAGG CGTGCGGCAA ATGCTTCAGC CAGAGTTCCA ATTTTCAGTG CCATCAGAGA
    GTCCACACGG AAGAGAAGCC CTACAAGTGT GAAGAGTGTG GCAAGGGCTT CGGCTGGAGC
    GTCAACCTCC GTGTTCACCA GCGGGTCCAC AGGGGCGAGA AACCCTACAA GTGTGAGGAG
    TGCGGGAAGG GCTTCACGCA GGCCGCCCAT TACCACATAC ACCAGAGGGT CCACACCGGG
    GAGAAGCCCT ATAAGTGCGA CGTCTGCGGC AAGGGGTTCA GTCACAACTC GCCTCTGATT
    TGCCACCGGC GCGTCCACAC TGGTGAGAAG CCCTACCGGT GCGAGGCCTG CGGCAAAGGC
    TTCACCCGGA ACACGGACCT CCACATCCAC TTCCGCGTCC ATACAGGGGA GAAGCCCTAC
    ACCTGCAAGG AGTGTGGGAA GGGCTTCAGT CAGGCTTCAA ATCTTCAAGT CCACCAGAAC
    GTCCACACTG GGGAGAAGCG GTTCAAGTGC GAGACATGCG GGAAGGGCTT CAGCCAGTCG
    TCCAAGCTCC AGACCCACCA GCGAGTCCAC ACCGGGGAGA AGCCCTACAG GTGCGACGTG
    TGTGGCAAGG ACTTCAGTTA CAGTTCCAAC CTGAAGCTGC ACCAGGTCAT TCACACCGGA
    GAGAAACCAT ACACCTGCGA GGCATGCGGG AAGGGCTTCA GCTGGAGGTC CAACCTTCAT
    GCTCATCAGC GAGTCCACTC GGGAGAGAAG CCCTACAAAT GTGAGGCGTG TGACAAGAGC
    TTCAGTCAGG CCATAGACTT CCGGGTCCAT CAGCGGGTCC ACACAGGCGA GAAACCCTAC
    AAGTGTGGTG TCTGCGGGAA GGGCTTCAGC CAGTCCTCGG GTCTTCAGTC CCATCAGAGG
    GTCCACACCG GGGAGAAGCC CTACAAATGC GATGTGTGTG GGAAGGGCTT CCGATACAGT
    TCCCAGTTTA TTTACCACCA GAGGGGCCAC ACTGGCGAAA AGCCTTACAA GTGTGAGGAG
    TGCGGGAAAG GCTTTGGGCG GAGCCTGAAC CTTCGCCACC ACCAGAGGGT CCACACAGGA
    GAGAAACCCC ACAAGTGTGA GGAGTGCGGG AAGGCCTTCA GTCTCCCCTC CAATCTTCGA
    GTCCACCTGA GCGTCCACAC CCGGGAGAAA CTGTTTAAAT GTGAGGATTG TGGGAAGGGC
    TTCAGTCAGA GTTCTCGGCT TCAAGCCCAC CAGAGGGTCC ACACAGGAGA AAAACCGTAC
    AAGTGCAACA TATGCGGTAA GGACTTCAGT CACCGTTCAC GGCTGACATA CCATCAGAAA
    GTCCACACTG GCAAGAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa42450
    Clone ID Related Accession (Same CDS sequence) NM_001077960.1 , NM_001077960.2
    Accession Version NM_001077960.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product zinc finger protein 227
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126592.1. On May 1, 2019 this sequence version replaced NM_001077960.1. ##Evidence-Data-START## Transcript exon combination :: BC126592.1, SRR5571257.48133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTTCTC AGGACTCTGA CTTTCCCCAG AAGGAACATG AGAAAATGAC CGAGTTTCAG 
    GAGGCCGTGA CCTTCAAGGA TGTGGCTGTG GTCTTCACTG AGGAGGAGCT GGGGTTACTG
    GACTCGGCCC AGAGGAAGCT GTACCAGGAT GTGATGGTGG AGAACTTCCG GAACCTGGTC
    TCAGTGGGAC ATGTTCCATT CAAAGCAGAT ATGGTATCCC AGTTGGAGAC AGAAGAAAAA
    CTGTGGACAA TGGAGAGGGA AACCCAAAGA AATGGGCATT CCAGTGAGAT CCACTCAGCT
    GTTACTTCAG GCAGCAAGAT CCCAAATGAG ATGGAGACTC TTGGGAAAGT CATGTTCAAA
    TACCTCTCGT GTGAAGAACT GTCCTGCTGG CAAATCTGGA AACACACCAC AAATGATTTA
    ACTCTGCAAA GGAAGAGTTC CTGGTTCCTG CATGGTGATT CTCTTCAAGT TTCTGAAGAT
    GAGAACCATA TAATGAATCA TAAAGGAGAT CACTTCGGTT GCCTTGAGAA TCAGGAGTTT
    TTGATTCCGA CAGCCCAGGC TTCCTGTGGG AGTAGGTGTC TGAGCGAGTC AGAGAATCCA
    AGCAGAGGTA AACAGATGAG TGTGAAAAAT CATCTGCATG TATGTGAAGG CTTCACAAAG
    AATTCACCGC TGAGCGATCC TGGTAAAACT GACGCAAAAC AGACACCCTG CAAAGGAGAG
    AGACCGCATC CCTGTAGAGT GTGTGGGGAG GGCTTCAGTC ATGGTGCAGT GCTCCCAGTT
    CATCAGGTTG ACCCTGGAGA AAAATGCTCC CATCTGCAGA CTCATCAGAG AATTCACCCA
    GGAGGGACTG TCAATAAATG TCCTAAATCT GGCGATGGTT TCCATCAGAA CTCCTTTCAC
    CCCCATCACT CCAACCCCAC AGGAGAAAAG TCCTACAGGT GTGACAGTTG TGGCAAGGCC
    TTTGGTAGCA GCACAGGCCT CATCATCCAT TACCGGACGC ACACAGGGGA GAAACCTTAC
    AGGTGCGAGG CGTGCGGCAA ATGCTTCAGC CAGAGTTCCA ATTTTCAGTG CCATCAGAGA
    GTCCACACGG AAGAGAAGCC CTACAAGTGT GAAGAGTGTG GCAAGGGCTT CGGCTGGAGC
    GTCAACCTCC GTGTTCACCA GCGGGTCCAC AGGGGCGAGA AACCCTACAA GTGTGAGGAG
    TGCGGGAAGG GCTTCACGCA GGCCGCCCAT TACCACATAC ACCAGAGGGT CCACACCGGG
    GAGAAGCCCT ATAAGTGCGA CGTCTGCGGC AAGGGGTTCA GTCACAACTC GCCTCTGATT
    TGCCACCGGC GCGTCCACAC TGGTGAGAAG CCCTACCGGT GCGAGGCCTG CGGCAAAGGC
    TTCACCCGGA ACACGGACCT CCACATCCAC TTCCGCGTCC ATACAGGGGA GAAGCCCTAC
    ACCTGCAAGG AGTGTGGGAA GGGCTTCAGT CAGGCTTCAA ATCTTCAAGT CCACCAGAAC
    GTCCACACTG GGGAGAAGCG GTTCAAGTGC GAGACATGCG GGAAGGGCTT CAGCCAGTCG
    TCCAAGCTCC AGACCCACCA GCGAGTCCAC ACCGGGGAGA AGCCCTACAG GTGCGACGTG
    TGTGGCAAGG ACTTCAGTTA CAGTTCCAAC CTGAAGCTGC ACCAGGTCAT TCACACCGGA
    GAGAAACCAT ACACCTGCGA GGCATGCGGG AAGGGCTTCA GCTGGAGGTC CAACCTTCAT
    GCTCATCAGC GAGTCCACTC GGGAGAGAAG CCCTACAAAT GTGAGGCGTG TGACAAGAGC
    TTCAGTCAGG CCATAGACTT CCGGGTCCAT CAGCGGGTCC ACACAGGCGA GAAACCCTAC
    AAGTGTGGTG TCTGCGGGAA GGGCTTCAGC CAGTCCTCGG GTCTTCAGTC CCATCAGAGG
    GTCCACACCG GGGAGAAGCC CTACAAATGC GATGTGTGTG GGAAGGGCTT CCGATACAGT
    TCCCAGTTTA TTTACCACCA GAGGGGCCAC ACTGGCGAAA AGCCTTACAA GTGTGAGGAG
    TGCGGGAAAG GCTTTGGGCG GAGCCTGAAC CTTCGCCACC ACCAGAGGGT CCACACAGGA
    GAGAAACCCC ACAAGTGTGA GGAGTGCGGG AAGGCCTTCA GTCTCCCCTC CAATCTTCGA
    GTCCACCTGA GCGTCCACAC CCGGGAGAAA CTGTTTAAAT GTGAGGATTG TGGGAAGGGC
    TTCAGTCAGA GTTCTCGGCT TCAAGCCCAC CAGAGGGTCC ACACAGGAGA AAAACCGTAC
    AAGTGCAACA TATGCGGTAA GGACTTCAGT CACCGTTCAC GGCTGACATA CCATCAGAAA
    GTCCACACTG GCAAGAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071428.1
    CDS229..2592
    Translation

    Target ORF information:

    RefSeq Version NM_001077960.2
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger protein 227 (ZNF227), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077960.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCTTCTC AGGACTCTGA CTTTCCCCAG AAGGAACATG AGAAAATGAC CGAGTTTCAG 
    GAGGCCGTGA CCTTCAAGGA TGTGGCTGTG GTCTTCACTG AGGAGGAGCT GGGGTTACTG
    GACTCGGCCC AGAGGAAGCT GTACCAGGAT GTGATGGTGG AGAACTTCCG GAACCTGGTC
    TCAGTGGGAC ATGTTCCATT CAAAGCAGAT ATGGTATCCC AGTTGGAGAC AGAAGAAAAA
    CTGTGGACAA TGGAGAGGGA AACCCAAAGA AATGGGCATT CCAGTGAGAT CCACTCAGCT
    GTTACTTCAG GCAGCAAGAT CCCAAATGAG ATGGAGACTC TTGGGAAAGT CATGTTCAAA
    TACCTCTCGT GTGAAGAACT GTCCTGCTGG CAAATCTGGA AACACACCAC AAATGATTTA
    ACTCTGCAAA GGAAGAGTTC CTGGTTCCTG CATGGTGATT CTCTTCAAGT TTCTGAAGAT
    GAGAACCATA TAATGAATCA TAAAGGAGAT CACTTCGGTT GCCTTGAGAA TCAGGAGTTT
    TTGATTCCGA CAGCCCAGGC TTCCTGTGGG AGTAGGTGTC TGAGCGAGTC AGAGAATCCA
    AGCAGAGGTA AACAGATGAG TGTGAAAAAT CATCTGCATG TATGTGAAGG CTTCACAAAG
    AATTCACCGC TGAGCGATCC TGGTAAAACT GACGCAAAAC AGACACCCTG CAAAGGAGAG
    AGACCGCATC CCTGTAGAGT GTGTGGGGAG GGCTTCAGTC ATGGTGCAGT GCTCCCAGTT
    CATCAGGTTG ACCCTGGAGA AAAATGCTCC CATCTGCAGA CTCATCAGAG AATTCACCCA
    GGAGGGACTG TCAATAAATG TCCTAAATCT GGCGATGGTT TCCATCAGAA CTCCTTTCAC
    CCCCATCACT CCAACCCCAC AGGAGAAAAG TCCTACAGGT GTGACAGTTG TGGCAAGGCC
    TTTGGTAGCA GCACAGGCCT CATCATCCAT TACCGGACGC ACACAGGGGA GAAACCTTAC
    AGGTGCGAGG CGTGCGGCAA ATGCTTCAGC CAGAGTTCCA ATTTTCAGTG CCATCAGAGA
    GTCCACACGG AAGAGAAGCC CTACAAGTGT GAAGAGTGTG GCAAGGGCTT CGGCTGGAGC
    GTCAACCTCC GTGTTCACCA GCGGGTCCAC AGGGGCGAGA AACCCTACAA GTGTGAGGAG
    TGCGGGAAGG GCTTCACGCA GGCCGCCCAT TACCACATAC ACCAGAGGGT CCACACCGGG
    GAGAAGCCCT ATAAGTGCGA CGTCTGCGGC AAGGGGTTCA GTCACAACTC GCCTCTGATT
    TGCCACCGGC GCGTCCACAC TGGTGAGAAG CCCTACCGGT GCGAGGCCTG CGGCAAAGGC
    TTCACCCGGA ACACGGACCT CCACATCCAC TTCCGCGTCC ATACAGGGGA GAAGCCCTAC
    ACCTGCAAGG AGTGTGGGAA GGGCTTCAGT CAGGCTTCAA ATCTTCAAGT CCACCAGAAC
    GTCCACACTG GGGAGAAGCG GTTCAAGTGC GAGACATGCG GGAAGGGCTT CAGCCAGTCG
    TCCAAGCTCC AGACCCACCA GCGAGTCCAC ACCGGGGAGA AGCCCTACAG GTGCGACGTG
    TGTGGCAAGG ACTTCAGTTA CAGTTCCAAC CTGAAGCTGC ACCAGGTCAT TCACACCGGA
    GAGAAACCAT ACACCTGCGA GGCATGCGGG AAGGGCTTCA GCTGGAGGTC CAACCTTCAT
    GCTCATCAGC GAGTCCACTC GGGAGAGAAG CCCTACAAAT GTGAGGCGTG TGACAAGAGC
    TTCAGTCAGG CCATAGACTT CCGGGTCCAT CAGCGGGTCC ACACAGGCGA GAAACCCTAC
    AAGTGTGGTG TCTGCGGGAA GGGCTTCAGC CAGTCCTCGG GTCTTCAGTC CCATCAGAGG
    GTCCACACCG GGGAGAAGCC CTACAAATGC GATGTGTGTG GGAAGGGCTT CCGATACAGT
    TCCCAGTTTA TTTACCACCA GAGGGGCCAC ACTGGCGAAA AGCCTTACAA GTGTGAGGAG
    TGCGGGAAAG GCTTTGGGCG GAGCCTGAAC CTTCGCCACC ACCAGAGGGT CCACACAGGA
    GAGAAACCCC ACAAGTGTGA GGAGTGCGGG AAGGCCTTCA GTCTCCCCTC CAATCTTCGA
    GTCCACCTGA GCGTCCACAC CCGGGAGAAA CTGTTTAAAT GTGAGGATTG TGGGAAGGGC
    TTCAGTCAGA GTTCTCGGCT TCAAGCCCAC CAGAGGGTCC ACACAGGAGA AAAACCGTAC
    AAGTGCAACA TATGCGGTAA GGACTTCAGT CACCGTTCAC GGCTGACATA CCATCAGAAA
    GTCCACACTG GCAAGAATCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.